CN101949840B - Quick and nondestructive detection method for myohemoglobin of raw meat and system thereof - Google Patents

Abstract

The invention discloses a quick and nondestructive detection method for myohemoglobin of raw meat and a system thereof. The method comprises the following steps: S1, radiating a standard sample by a light source to ensure that the light is incident from the surface to the inside of the sample, and is diffused from the surface of the standard sample; S2, receiving and detecting the diffused diffuse light intensity; S3, inverting a system constant alpha by using the solution of the diffusion equation; S4, radiating the raw meat sample to be detected by light to ensure that the light is incident from the surface to the inside of the raw meat sample to be detected, and is diffused from the surface of the raw meat sample to be detected; S5, receiving and detecting the diffused diffuse light intensity; S6, calculating a reduced scattering coefficient mu s' and an absorption coefficient mu a of the raw meat sample to be detected; S7, repeating the step S4 to S6 for incident lights with different wavelengths to acquire reduced scattering coefficients mu s' and an absorption coefficients mu a of the raw meat sample to be detected in a plurality of wavelengths; and S8, calculating the concentration of the myohemoglobin of the raw meat according to the absorption coefficient equation in a plurality of wavelength positions. The method can realize direct, non-destructive and quick testing on the myohemoglobin inside the raw meat, and has high accuracy.

Description

Raw meat myoglobins fast non-destructive detection method and system

Technical field

The embodiment of the invention relates to agriculture sensing detection technical field, especially a kind of raw meat myoglobins fast non-destructive detection method and system.

Background technology

Along with the raising of people's living standard, the amount of livestock and poultry fresh meat increases rapidly, and then the quality of meat is had higher requirement.Yellowish pink be in several big key elements of meat sold on the market color, matter the guide of intuition experience impression, therefore the color of cooling off meat is one of most important organoleptic indicator of consumer evaluation's freshness, the color of meat mainly is to be distributed by three kinds of myoglobins in the meat different chemical existence forms (being the metmyoglobin of bolarious reduced form myoglobins, cherry oxymyoglobin and brown) and a spot of haemoglobin to determine that these materials have absorption in the near-infrared spectra district to light.Because meat self-dissolving, corruption, also sex change of myoglobins, decomposition cause the meat change color.

Muscle shade and tone (colourity) depend on the muscle pigment content.The muscle pigment mainly is made up of myoglobins and haemoglobin and micro-coloured metabolin.If when adopting the sensory testing, often contain subjective factor, this has just influenced the correct evaluation to meat quality undoubtedly.Color board method and physics method can only be measured meat sample surface color, belong to two dimensional surface tolerance.Chemical assay is that the meat sample is carried out 3 D stereo tolerance, surveys the total pigment content of meat sample.Meat sample pigment quantivative approach has Hornsey method etc., and its principle all is to extract earlier the back colorimetric, be sampling diminish measuring method and Measuring Time is about 2 hours.

Although the existing trial that obtains the myoglobins of pork with the optics loseless method in the prior art, but there is following problem in this method: suppose that 1. reduced scattering coefficient is known or constant, but the reduced scattering coefficient of actual each sample is all different, and simple hypothesis can not be measured real myoglobin concentration; 2. suppose that myoglobins and haemoglobin have identical molar absorption coefficient ε, measurement be two kinds of material concentration sums of myoglobins and haemoglobin, can't obtain the concentration of myoglobins.

Summary of the invention

(1) technical matters that will solve

The technical problem to be solved in the present invention first: how to realize directly, can't harm, detect apace the myoglobins of raw meat inside, and improve accuracy rate.

(2) technical scheme

For solving the problems of the technologies described above, the invention provides a kind of raw meat myoglobins fast non-destructive detection method, may further comprise the steps:

S1, the constant light source irradiation standard model of usefulness light intensity make light incide inside from its surface, go through the optical path of the complications of absorption and scattering process, and come out from this standard model surface diffusion; The reduced scattering coefficient μ of wherein said standard model _s' and absorption coefficient μ _aKnown;

S2, reception also detect the diffusion light intensity that diffusion is come out;

S3, with the reduced scattering coefficient μ of detected diffusion light intensity, described standard model _s' and absorption coefficient μ _aThe substitution diffusion equation separate the system constants α that is finally inversed by in the described diffusion equation;

S4, usefulness irradiate light raw meat testing sample make light incide its inside from the surface, go through the tortuous optical path of absorption and scattering process, come out from this raw meat testing sample surface diffusion;

S5, reception also detect the diffusion light intensity that diffusion is come out;

S6, utilize system constants α, reduced scattering coefficient μ _s' and absorption coefficient μ _aExpression formula and two positions on receive and detect the reduced scattering coefficient μ that the diffusion light intensity that obtains calculates the raw meat testing sample _s' and absorption coefficient μ _a

S7, to the incident light of different wave length, repeating step S4～S6 obtains the reduced scattering coefficient μ of raw meat testing sample at a plurality of wavelength place _s' and absorption coefficient μ _a

S8, the absorption coefficient μ that utilizes step S7 to calculate _a, according to the myoglobin concentration of the absorption coefficient Equation for Calculating raw meat at a plurality of wavelength place.

Preferably, described light source is polychromatic light a kind of in the light that produces of the light that produces of the light that shoots out of color chips, LED and semiconductor laser after filtration.

Preferably, receive described diffusion light intensity respectively, at least at distance light source 20mm place, be spaced apart 1mm-10mm between the optical fiber apart from the nearest optical fiber of light source with the multifiber at diverse location place; The check point of the multifiber at light source and diverse location place point-blank.。

Wherein, the diffusion light intensity that receives is detected comprise: light intensity signal is converted into analog electrical signal, and this model electric signal is changed into digital quantity.

Wherein, described diffusion equation is the stable state diffusion equation, described stable state diffusion equation separate for:

$I\left(\mathrm{\ρ}\right)=\frac{\mathrm{\α}}{4\mathrm{\π}{\mathrm{\μ}}_t{\mathrm{\ρ}}^2}({\mathrm{\μ}}_{\mathrm{eff}}+\frac{1}{\mathrm{\ρ}})e^{-{\mathrm{\ρ\μ}}_{\mathrm{eff}}},{\mathrm{\μ}}_{\mathrm{eff}}=\sqrt{3{\mathrm{\μ}}_a({\mathrm{\μ}}_a+{\mathrm{\μ}}_s^{\′})},{\mathrm{\μ}}_t={\mathrm{\μ}}_a+{\mathrm{\μ}}_s^{\′}$

Wherein, ρ is the distance between described light source and the optical fiber, and I (ρ) is the diffusion light intensity at ρ place, μ _EffEffective attenuation factor for described raw meat.

Wherein, step S8 is specially:

Find the solution following 4 yuan of 1 sublinear equation, calculate reduction myoglobin concentration C1, oxymyoglobin concentration C 2, reduced hemoglobin concentration C 3 and oxyhemoglobin concentration C 4,

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_1}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_1}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_1}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_1}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_1}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_2}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_2}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_2}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_2}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_2}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_3}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_3}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_3}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_3}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_3}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_4}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_4}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_4}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_4}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_4}C4$

Wherein, λ _i, i=1,4 wavelength of 2,3,4 expressions, Be the molar absorption coefficient of the various albumen under the different wave length,

Absorption coefficient for described raw meat under the different wave length;

The myoglobin concentration of described raw meat is C1 and C2 sum.

Preferably, described standard model is the silica gel solid.

In addition, the present invention also provides a kind of raw meat myoglobins quick nondestructive detection system that is used to realize said method, comprising:

Light source is used to shine standard model and raw meat testing sample, makes light incide its inside from the surface, goes through the tortuous optical path of absorption and scattering process, comes out from standard model and raw meat testing sample surface diffusion;

Multifiber at least at distance light source 20mm place, is used to receive the diffusion light intensity that diffusion goes out apart from the nearest optical fiber of light source;

A plurality of photoelectric detectors are connected one to one with described optical fiber, be used for the light intensity signal that optical fiber receives is converted into analog electrical signal, and this model electric signal is changed into digital quantity;

Calculating treatmenting equipment connects described a plurality of photoelectric detector, is used for the myoglobin concentration according to the detected calculated signals raw meat of described photoelectric detector.

(3) beneficial effect

Advantage of the present invention is: adopt the diffuse reflection mode based on the organism optical spectral technique, obtain the diffusion light intensity of light source and different detection distances, utilize separate (this separates the relation that detects distance, absorption coefficient, reduced scattering coefficient that reflected) of diffusion equation directly to calculate absorption coefficient, and utilize relation between absorption coefficient and the myoglobin concentration, can be directly, harmless, detect the myoglobins of raw meat inside rather than two kinds of concentration that material mixes apace; Employing can be carried out the total pigment content detection of 3 D stereo tolerance to the meat sample based on the stable state spatial spectral technology and the fibre-optical probe of organism optical, detects the degree of depth and can reach 1cm-1.5cm; Need not to suppose that reduced scattering coefficient is known or constant, need not to suppose that myoglobins and haemoglobin have identical molar absorption coefficient, energy measurement goes out real myoglobin concentration, the accuracy rate height; Adopt the analytical approach of organism optical to realize the non-destructive fast detecting, experiment showed, detection time less than 5 seconds, safety easy to use, reliable and stable, selectivity is strong, is suitable for on-the-spot the detection.

Description of drawings

Fig. 1 is the schematic flow sheet of the method for the embodiment of the invention;

Fig. 2 is the structural representation Fig. 1 and the use signal thereof of the system of the embodiment of the invention;

Fig. 3 is the components of system as directed parts structural representation of the embodiment of the invention;

Fig. 4 is structural representation Fig. 2 of embodiment of the invention system and uses signal.

Embodiment

For making purpose of the present invention, content and advantage clearer, embodiment of the present invention is described further in detail below in conjunction with accompanying drawing.

The method of the embodiment of the invention adopts based on the stable state spatial spectral technology of organism optical and fibre-optical probe can carry out the total pigment content detection of 3 D stereo tolerance to the meat sample, wherein, the embodiment of stable state spatial spectral technology is: use the constant light source of light intensity, and the check point of light source and optical fiber leaves certain distance and point-blank.In, light is from the incident of meat tissue surface, because the meat tissue is to the scattering and the absorption of light, the diffused light that detects on the surface of meat tissue carries the inner structure and the content of material information of abundant meat tissue, therefore can obtain and absorption, the corresponding absorption coefficient μ of scattering by the space distribution (being the diffusion light intensity) of measuring the tissue surface diffused light _a, reduced scattering coefficient μ ' _sDeng the tissue optical property parameter.

Fig. 1 is the schematic flow sheet of raw meat myoglobin content fast non-destructive detection method embodiment of the present invention, and as shown in Figure 1, method embodiment of the present invention comprises the steps:

The light of step 101, light source incides the optics road warp that the complications of absorption and scattering process are gone through in its inside by standard (thing) sample surfaces, comes out from the sample surfaces diffusion; Wherein, standard model can be the silica gel solid with similar reduced scattering coefficient to raw meat, absorption coefficient.

Step 102, on the standard model surface, respectively the diffused light of two detection position outgoing is received with two optical fiber at a distance of ρ 1, ρ 2 places with light source, two optical fiber are connected to different detecting devices respectively;

Step 103, the signal process calculating treatmenting equipment that is detected are finally inversed by constant alpha with the known absorbing coefficient of standard model and the solution formula of reduced scattering coefficient substitution diffusion equation;

Step 104, light source light incide the optics road warp that the complications of absorption and scattering process are gone through in its inside by raw meat testing sample surface, go out from the sample surfaces diffusion;

Step 105, on raw meat testing sample surface, respectively the diffused light of two detection position outgoing is received with two optical fiber at a distance of ρ 1, ρ 2 places with light source, two optical fiber are connected to different detecting devices respectively;

Step 106, the signal process calculating treatmenting equipment that is detected can utilize diffusion light intensity and absorption coefficient and reduced scattering coefficient expression formula on two positions, separate the absorption coefficient μ that Simultaneous Equations can obtain the raw meat testing sample _a, reduced scattering coefficient μ ' _s

Step 107, to the incident light of different wave length, repeating step 104-106 obtains the absorption coefficient μ at 4 wavelength places _a, reduced scattering coefficient μ ' _s

Step 108, according to μ _aWith the relation of myoglobins, list 4 yuan of once linear equations, find the solution the concentration that obtains myoglobins.

Can adopt wavelength in the embodiment of the invention is 660nm, 760nm, and 850nm, four LED of 970nm are as light source.

The raw meat myoglobin content fast non-destructive detection method that the embodiment of the invention provides, obtain to have the diffusion light intensity data of depth information by optical fiber and testing circuit, utilize diffusion light intensity and reduced scattering coefficient and absorption coefficient to realize the deep layer information of detection meat sample interior; Resolve the raw meat myoglobin content according to absorption coefficient.

Fig. 2 is structural representation Fig. 1 of system embodiment of the present invention and uses signal (a top width of cloth is to detect the raw meat testing sample, below a width of cloth be to detect the raw meat standard model), shown in Fig. 1～2, in the above-mentioned steps:

1. in order to obtain constant alpha:

Adopt aluminium block to be used for fixing light source 204 and optical fiber 2071,2072 as support 201;

204 timesharing of light source driving units 202 driving light sources produce 4 tunnel drive signals, and each road signal is the 10mA electric current; Drive through 203 pairs of light sources 204 of lead;

Light enters that standard model 210, light absorb therein, scattering, can form light path track 2111,2112, and in distance light source 204 ρ 1, ρ 2 places outgoing, in the present embodiment, ρ 1=22mm, ρ 2=25mm, receive the diffused light at ρ 1 place with optical fiber 2071, detect light intensity by photoelectric detector 2081, detecting device is integrated photo detector OPT211, receive the diffused light at ρ 2 places with optical fiber 2072, detect light intensity by photoelectric detector 2082, detecting device is integrated photo detector OPT211, calculates α through calculating treatmenting equipment 209;

2. in order to obtain four absorption coefficients under the wavelength:

Adopt aluminium block to be used for fixing light source 204 and optical fiber 2071,2072 as support 201;

204 timesharing of light source driving units 202 driving light sources produce 4 tunnel drive signals, and each road signal is the 10mA electric current; Drive through 203 pairs of light sources 204 of lead;

Light enters that raw meat testing sample 206, light absorb, scattering in sample, can form light path 2051,2052, and in distance light source 204 ρ 1, ρ 2 places outgoing, receive the diffused light at ρ 1 place with optical fiber 2071, detect light intensity by photoelectric detector 2081, detecting device is integrated photo detector OPT211, receive the diffused light at ρ 2 places with optical fiber 2072, detect light intensity by photoelectric detector 2082, detecting device is integrated photo detector OPT211, and process calculating treatmenting equipment 209 calculates the absorption coefficient μ under each wavelength _a

As shown in Figure 3, system embodiment of the present invention comprises: input-output device 31 and calculating treatmenting equipment 32.

Wherein, the diffusion light intensity analog signal conversion that D/A converting circuit 311 in the input-output device 31 (can be included in the photoelectric detector) will receive testing circuit input in the photoelectric detector is a digital signal, 12 ADC that it can adopt C8501F020 to carry; Calculating treatmenting equipment 32 obtains the absorption coefficient of constant alpha and raw meat testing sample according to separating of diffusion light intensity data and diffusion equation; Calculating treatmenting equipment 32 goes out myoglobin content according to four kinds of wavelength absorption coefficient calculations.Input-output device 31 also comprises display 312, is used to show result of calculation.

Calculating treatmenting equipment 32 comprises: first computing unit 321, second computing unit 322 and storer 323.

Wherein, first computing unit 321 is used for calculating according to following formula the absorption coefficient μ of raw meat testing sample _aWith reduced scattering coefficient μ ' _s:

$I\left(\mathrm{\ρ}\right)=\frac{\mathrm{\α}}{4\mathrm{\π}{\mathrm{\μ}}_t{\mathrm{\ρ}}^2}({\mathrm{\μ}}_{\mathrm{eff}}+\frac{1}{\mathrm{\ρ}})e^{-{\mathrm{\ρ\μ}}_{\mathrm{eff}}}---\left(1\right)$

${\mathrm{\μ}}_{\mathrm{eff}}=\sqrt{3{\mathrm{\μ}}_a({\mathrm{\μ}}_a+{\mathrm{\μ}}_s^{\′})}---\left(2\right)$

μ _t＝μ _a+μ′ _s (3)

For the raw meat testing sample, and μ ' _s＞＞μ _aThe time, then obtain Behind the I (ρ 1), the I (ρ 2) that obtain two detection distances, can calculate absorption coefficient μ _a, reduced scattering coefficient μ ' _s

Second computing unit 322 is used to utilize the absorption coefficient μ of four wavelength _aConcentration relationship (shown in following three formula) with myoglobins, haemoglobin, find the solution following quaternary once linear system of equations, calculate reduction myoglobin concentration C1, oxymyoglobin concentration C 2, reduced hemoglobin concentration C 3, oxyhemoglobin concentration C 4.

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_1}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_1}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_1}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_1}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_1}C4---\left(4\right)$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_2}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_2}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_2}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_2}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_2}C4---\left(5\right)$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_3}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_3}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_3}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_3}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_3}C4---\left(6\right)$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_4}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_4}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_4}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_4}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_4}C4---\left(7\right)$

In the following formula, λ _i, i=1,2,3,4 expressions 4 wavelength 660nm, 760nm, 850nm, 970nm,

${\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_{660}}=0.171m{M}^{-1}m{m}^{-1},{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_{660}}=0.0165m{M}^{-1}{\mathrm{mm}}^{-1},{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_{660}}=0.344m{M}^{-1}{\mathrm{mm}}^{-1},$

${\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_{660}}=0.334m{M}^{-1}m{m}^{-1},{\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_{760}}=0.083m{M}^{-1}{\mathrm{mm}}^{-1},{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_{760}}=0.0342m{M}^{-1}{\mathrm{mm}}^{-1},$

${\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_{760}}=0.167m{M}^{-1}m{m}^{-1},{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_{760}}=0.06906m{M}^{-1}{\mathrm{mm}}^{-1},{\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_{850}}=0.039m{M}^{-1}{\mathrm{mm}}^{-1},$

${\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_{850}}=0.057m{M}^{-1}m{m}^{-1},{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_{850}}=0.07861m{M}^{-1}{\mathrm{mm}}^{-1},{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_{850}}=0.116m{M}^{-1}{\mathrm{mm}}^{-1},$

${\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_{970}}=0.0255m{M}^{-1}m{m}^{-1},{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_{970}}=0.064m{M}^{-1}{\mathrm{mm}}^{-1},{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_{970}}=0.05166m{M}^{-1}{\mathrm{mm}}^{-1},$

It is respectively the molar absorption coefficient of various albumen under the different wave length; Myoglobin concentration is C1 and C2 sum.Experiment shows that it is 24 μ M that pig longissimus dorsi muscle sample is detected myoglobin concentration.

Calculating treatmenting equipment 32 also comprises: storer 323; The myoglobin concentration value that storer 323 is preserved results of intermediate calculations and calculated.

Fig. 4 is the structural representation Fig. 2 and the use signal thereof of the system of the embodiment of the invention, as shown in Figure 4, the embodiment of the invention comprises: light source and driver element 41, meat sample 42, optical fiber and testing circuit 43, central control unit 44, signal processing unit 45, data storage cell 46, processor 47; Wherein, unit 43, central control unit 44 that optical fiber and testing circuit form, signal processing unit 45, data storage cell 46 can be realized the function that Fig. 2 and middle diffused light embodiment illustrated in fig. 3 detect; Processor 47 can be realized the function of Fig. 2 and middle calculating treatmenting equipment embodiment illustrated in fig. 3.

Wherein, light source and driver element 41 impinge perpendicularly on the surface of meat sample 42 by optical fiber, at meat sample 42 surface distance light source center different distance ρ 1, ρ 2 places 2 optical fiber are arranged, light obtains analog electrical signal by the OPT211 integrated photo detector of testing circuit 43, analog electrical signal is sent to signal processing unit 45, signal processing unit 45 carries out the signal modulation with analog electrical signal, transformation result is read by central control unit 44, and provides the driving of driving timing sequence generating to light source by central control unit 44.

Among the invention described above embodiment, testing circuit 43 obtains having the diffused light data of depth information according to light, has realized the detection to meat sample interior deep layer information; Central control unit 44 can calculate the raw meat myoglobin content, has realized that the meat sample is carried out quick nondestructive to be detected.

One of ordinary skill in the art will appreciate that: all or part of step that realizes the foregoing description can be finished by the relevant hardware of programmed instruction, aforesaid program can be stored in the computer read/write memory medium, this program is carried out the step that comprises said method embodiment when carrying out; And aforesaid storage medium comprises: various media that can be program code stored such as ROM, RAM, magnetic disc or CD.

Above embodiment only is used to illustrate the present invention; and be not limitation of the present invention; the those of ordinary skill in relevant technologies field; under the situation that does not break away from the spirit and scope of the present invention; can also make various variations and modification; therefore all technical schemes that are equal to also belong to category of the present invention, and scope of patent protection of the present invention should be defined by the claims.

Claims (6)

1. a raw meat myoglobins fast non-destructive detection method is characterized in that, may further comprise the steps:

S1, the constant light source irradiation standard model of usefulness light intensity make light incide inside from its surface, go through the optical path of the complications of absorption and scattering process, and come out from this standard model surface diffusion; The reduced scattering coefficient μ of wherein said standard model _s' and absorption coefficient μ _aKnown;

S2, reception also detect the diffusion light intensity that diffusion is come out;

S3, with the reduced scattering coefficient μ of detected diffusion light intensity, described standard model _s' and absorption coefficient μ _aThe substitution diffusion equation separate the system constants α that is finally inversed by in the described diffusion equation;

S4, usefulness irradiate light raw meat testing sample make light incide its inside from the surface, go through the tortuous optical path of absorption and scattering process, come out from this raw meat testing sample surface diffusion;

S5, reception also detect the diffusion light intensity that diffusion is come out, and wherein, receive described diffusion light intensity respectively with the multifiber at diverse location place;

S6, utilize system constants α, reduced scattering coefficient μ _s' and absorption coefficient μ _aExpression formula and two positions on receive and detect the reduced scattering coefficient μ that the diffusion light intensity that obtains calculates the raw meat testing sample _s' and absorption coefficient μ _a

S7, to the incident light of different wave length, repeating step S4～S6 obtains the reduced scattering coefficient μ of raw meat testing sample at a plurality of wavelength place _s' and absorption coefficient μ _a

S8, the absorption coefficient μ that utilizes step S7 to calculate _a, according to the myoglobin concentration of the absorption coefficient Equation for Calculating raw meat at a plurality of wavelength place;

Described diffusion equation is the stable state diffusion equation, described stable state diffusion equation separate for:

$I\left(\mathrm{\ρ}\right)=\frac{\mathrm{\α}}{{4\mathrm{\π\μ}}_t{\mathrm{\ρ}}^2}({\mathrm{\μ}}_{\mathrm{eff}}+\frac{1}{\mathrm{\ρ}})e^{-{\mathrm{\ρ\μ}}_{\mathrm{eff}}},$ ${\mathrm{\μ}}_{\mathrm{eff}}=\sqrt{{3\mathrm{\μ}}_a({\mathrm{\μ}}_a+{\mathrm{\μ}}_s^{\′})},$ μ _t＝μ _a+μ′ _s

Wherein, ρ is the distance between described light source and the optical fiber, and I (ρ) is the diffusion light intensity at ρ place, μ _EffEffective attenuation factor for described raw meat;

Step S8 is specially:

Find the solution following 4 yuan of 1 sublinear equation, calculate reduction myoglobin concentration C1, oxymyoglobin concentration C 2, reduced hemoglobin concentration C 3 and oxyhemoglobin concentration C 4,

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_1}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_1}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_1}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_1}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_1}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_2}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_2}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_2}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_2}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_2}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_3}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_3}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_3}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_3}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_3}C4$

${\mathrm{\μ}}_a^{{\mathrm{\λ}}_4}={\mathrm{\ϵ}}_1^{{\mathrm{\λ}}_4}C1+{\mathrm{\ϵ}}_2^{{\mathrm{\λ}}_4}C2+{\mathrm{\ϵ}}_3^{{\mathrm{\λ}}_4}C3+{\mathrm{\ϵ}}_4^{{\mathrm{\λ}}_4}C4$

Wherein, λ _i, i=1,4 wavelength of 2,3,4 expressions,

Be the molar absorption coefficient of the various albumen under the different wave length,

Absorption coefficient for described raw meat under the different wave length;

The myoglobin concentration of described raw meat is C1 and C2 sum.

2. raw meat myoglobins fast non-destructive detection method as claimed in claim 1 is characterized in that, described light source is polychromatic light a kind of in the light that produces of the light that produces of the light that shoots out of color chips, LED and semiconductor laser after filtration.

3. raw meat myoglobins fast non-destructive detection method as claimed in claim 1 is characterized in that, at least at distance light source 20mm place, is spaced apart 1mm-10mm between the optical fiber apart from the nearest optical fiber of light source; The check point of the multifiber at light source and diverse location place point-blank.

4. raw meat myoglobins fast non-destructive detection method as claimed in claim 3 is characterized in that, the diffusion light intensity that receives is detected comprise: light intensity signal is converted into analog electrical signal, and this model electric signal is changed into digital quantity.

5. as each described raw meat myoglobins fast non-destructive detection method of claim 1～4, it is characterized in that described standard model is the silica gel solid.

6. a raw meat myoglobins quick nondestructive detection system that is used for realizing each described method of claim 1～4 is characterized in that, comprising:

Light source is used to shine standard model and raw meat testing sample, makes light incide its inside from the surface, goes through the tortuous optical path of absorption and scattering process, comes out from standard model and raw meat testing sample surface diffusion;

Multifiber at least at distance light source 20mm place, is used to receive the diffusion light intensity that diffusion goes out apart from the nearest optical fiber of light source;

A plurality of photoelectric detectors are connected one to one with described optical fiber, be used for the light intensity signal that optical fiber receives is converted into analog electrical signal, and this model electric signal is changed into digital quantity;

Calculating treatmenting equipment connects described a plurality of photoelectric detector, is used for the myoglobin concentration according to the detected calculated signals raw meat of described photoelectric detector.

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