CN101948766B - Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin - Google Patents
Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin Download PDFInfo
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Abstract
The invention relates to a process method for for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin. The method comprises the following steps of: taking waste residues generated by deeply fermenting Wuyiencin, bean flour of which the weight is 8-12 relative to the weight of the waste residues, corn meal of which the weight is 18-32 relative to the weight of the waste residues, starch of which the weight is 3-8 percent relative to the weight of the waste residues and fish meal of which the weight is 3-5 percent relative to the weight of the waste residues; sterilizing and disinfecting with steam to obtain a solid fermentation medium; stirring and mixing the sterilized solid fermentation medium and the waste residues to obtain a solid culture material; and spreading the solid culture material on a culture bed by controlling the water content of the culture material to be 30-40 percent and the temperature to be 30-45 DEG C to obtain a solid culture material containing Wuyi biocontrol bacterial spores, wherein the spore content is 40-50 hundred millions/gram. The invention has low production cost, reduces the discharge of waste in the production process of the Wuyiencin, has no waste pollutants in the production process and is environmentally friendly. Proved by tests, the Wuyi biocontrol bacteria have obvious effects of disease resistance, stress resistance and production increase.
Description
Technical field
The present invention relates to the process method of a kind of production Wuyi biocontrol microorganisms, specifically, relate to a kind of process method of utilizing the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, belong to biological technical field.
Background technology
Research shows that plant itself has the degeneration-resistant and immunologic function and the defense mechanism to disease and pest of heredity.When utilizing the activity of mikrobe to be used for plant protection, the direct bactericidal of the microbiotic of existing generation.Polysaccharide, polypeptide that also useful useful microbe produces, what in degradation process, produce conducts and participation, but is called as plant inducible system resistance crop inducing anti-disease, degeneration-resistant signal.It is the plant protection pattern of unique target that this kind research has changed with sterilization, has become the important means of Sustainable development agricultural, also is the inevitable choice of Organic farming technology.
It is the bacterial strain that cultivation is found in China native country that Wuyiencin produces bacterium, has the uniqueness and the novelty of its species, meta-bolites and genetic resources, and the application potential that is contained is very considerable, usually Wuyiencin is used as microbiotic.Utilize Wuyiencin to produce bacterium, abbreviate the Wuyi biocontrol microorganisms usually as, can prevent and treat soil-borne disease of crop as biological control agents.To the control of eggplant verticillium wilt, cotton wilt, irritate root, good preventive effect all arranged after spreading manuer in holes.Its principle is not only and has been utilized the Wuyi biocontrol microorganisms to seize the niche of pathogenic bacteria and the sterilizing ability of biocontrol microorganisms secretory substance; The mycelia of raised growth after the spore germination of while biocontrol microorganisms; Its cell can form a large amount of polysaccharide materials behind the root of the crop superficial degradation, pass through a series of vital movement and cross-conduction again; Impel the root of crop, the self-defense response that stem forms degeneration-resistant disease; Reached the effect of disease prevention, promptly the Wuyi biocontrol microorganisms produced antibacterial with induce the ability of crop disease-resistant, reached the effect of biological control.
The Wuyi bacterium has very strong field planting ability in plant materialss such as root of the crop cauline leaf; Can stop the pathogen infection plant through the site competition; The Wuyi biocontrol microorganisms itself is a kind of streptomycete that China cultivates; The Wuyi biocontrol microorganisms is to control bacterium with bacterium, through the broad-spectrum antimicrobial material that the Wuyi biocontrol microorganisms produces, and ecological site competition and induce mechanism such as crop resistance to reach the purpose of preventing and treating disease.The Wuyi biocontrol microorganisms has very strong field planting ability in plant materialss such as root, stem and leaf, can stop pathogenic bacteria infringement plant through the site competition; Enclose the broad-spectrum antimicrobial material that continuous secretion is come out with the intravital Wuyi of plant biocontrol microorganisms at the roots of plants primitive period simultaneously and can suppress or kill pathogenic bacteria, can directly destroy the cell walls of pathogenic bacteria, its body fluid is leaked; Wuyi biocontrol microorganisms excretory exocellular polysaccharide ability inducing plant produces the ability of resistance and some germ of passivation and virus infraction in addition.
Adopt the Wuyiencin submerged fermentation to produce in the antibiotic filtration operation; After utilizing the plate-and-frame filter press extruding to obtain containing the antibiotic clear liquid in Wuyi; Remaining waste residue is normally transported in the field by peasant household, and the back of rotting is as farmland fertilizer; But waste residue can produce peculiar smell, contaminate environment in process is rotted in fermentation; In addition, waste residue contain the nutrition source, Wuyi bacterium mycelium, the sophisticated spore of Wuyi bacterium of not complete digestion, a small amount of Wuyi bacterium exopolysaccharide etc. all corruption cause waste, handle and the transportation waste residue also will increase production cost.
Summary of the invention
The problem that the present invention will solve provides a kind of process method of utilizing the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, adopts this method production Wuyi biocontrol microorganisms, has made full use of the waste residue that produces in the Wuyiencin submerged fermentation, low, the environmental protection of production cost.
For overcoming the above problems; Technical scheme of the present invention is: utilize the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, it is characterized in that: said process method comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: get the waste residue that the Wuyiencin submerged fermentation produces;
(2) solid-state fermentation culture medium procedure for preparation: the bean powder that to get relative waste residue weight be 8-12%, relatively waste residue weight be 18-32% Semen Maydis powder, waste residue weight is the starch of 3-8% and the fish meal that relative waste residue weight is 3-5% relatively; Carry out the steam sterilizing sterilization; Vapor pressure 0.3MPa; Time 40-60 minute, obtain solid-state fermentation culture medium;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium and waste residue after will sterilizing mix, and obtain solid-state cultivation material;
(4) solid-state cultivation operation: with solid-state cultivation material be laid in culture bed on, the water cut of cultivating material is controlled at 30-40%, temperature is controlled at 30-45 ℃, obtains containing the solid-state cultivation material of Wuyi biocontrol microorganisms spore, spore content is 40-50 hundred million/gram.
Further improvement as technique scheme:
Said process method also comprises:
(5) drying and crushing operation: solid-state cultivation material is taken off from culture bed, carry out drying, pulverization process, obtain the solid-state cultivation material of granular Wuyi biocontrol microorganisms.
In the said maintenance bacterial classification spore operation, waste residue was sent into aseptic proportioning room in 2 hours.
In the said solid-state fermentation culture medium procedure for preparation, vapor pressure is 0.3MPa, and the steam sterilizing disinfecting time is 40-60 minute.
The present invention adopts above technical scheme; Have the following advantages: made full use of the waste residue that produces in the Wuyiencin submerged fermentation, production cost is low, has reduced discharging wastes in the Wuyiencin production process simultaneously; Do not produce waste pollutant, environmental protection in the production process.Through test, Wuyi of the present invention biocontrol microorganisms has significantly disease-resistant, degeneration-resistant and production-increasing function.
Below in conjunction with embodiment the present invention is further specified.
Embodiment
Embodiment 1, utilizes the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, and the Wuyiencin submerged fermentation is used for preparing in the antibiotic process of Wuyi bacterium; In fermentor tank, drop into earlier 10% substratum, steam goes out behind the assorted bacterium, introduces the Wuyiencin bacterial classification; Carry out deep fermentation; Temperature is controlled at 35 ℃, ferments to make 65 hours the time to contain the antibiotic fermented feed liquid of Wuyi bacterium, utilizes plate-and-frame filter press to squeeze out again and contains the antibiotic clear liquid of Wuyi bacterium in order to extracting the product of Wuyiencin; Plate-and-frame filter press is the XX type pressure filter that Jinan machine factory produces; The waste residue that squeezes out in the board and frame machine is pie more, and nutrition source, Wuyi bacterium mycelium, the sophisticated spore of Wuyi bacterium and a small amount of Wuyi bacterium exopolysaccharide that includes few not complete digestion is the bacterial classification source of production Wuyi biocontrol microorganisms.
The process method of Wuyi biocontrol microorganisms comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: send into aseptic proportioning room after the waste residue of above-mentioned plate-and-frame filter press extrusion was cleared up in 2 hours;
(2) solid-state fermentation culture medium procedure for preparation: get the Semen Maydis powder of the bean powder of relative waste residue weight 12%, relative waste residue weight 32%, the starch of relative waste residue weight 5% and the fish meal of relative waste residue weight 4%; Utilize high-pressure steam sterilizing pan to carry out the layering sterilization; Vapor pressure 0.3MPa, disinfecting time 50 minutes obtains solid-state fermentation culture medium; The high-pressure steam sterilizing pan model is a GT7C2000 type series horizontal sterilizer, and manufacturer is a Wuxi City food mechanical equipment factory;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium after will sterilizing and the waste residue cake that contains Wuyi biocontrol microorganisms bacterial classification; The mechanical stirring thorough mixing; Obtain solid-state cultivation material, use JMF-140 type stirrer, manufacturer is a Wuxi City food mechanical equipment factory;
(4) solid-state cultivation operation: the solid-state cultivation material that will stir evenly be laid in culture bed on, culture bed is the multilayer shelf, the gauge control of solid-state cultivation material is at 6.8 centimetres; Watering makes the water cut of solid-state cultivation material be controlled at 30-40%, and culture temperature is controlled at 30-45 ℃; Cultivate the material surface when being covered with a large amount of mycelia; To cultivate material and all turn over one time, all accept air and moisture up and down so that cultivate material, so that mycelia enlarges increment on the surface; Incubation time 65 hours; Obtain containing the mycelial solid substances of a large amount of Wuyi biocontrol microorganisms spores, this Wuyi biocontrol microorganisms is measured its spore amount with blood platelet counting plate and is reached 40-50 hundred million/gram, promptly reaches training objective;
(5) drying and crushing operation: the solid fermentation late stage of culture, moisture content can drop to 20-30%, is than rarefaction, carries out drying and pulverization process easily.To reach the whole solid-state culture of cultivating the spore amount takes off from culture bed; Concentrate and send into the drying plant, utilize the fast rotational flash dryer, the solid fermentation thing is blowed floating; Rotation, shearing, collision, friction and micronize; Dry, pulverize, screening once accomplishes, and guaranteed that Wuyi biocontrol microorganisms and spore being no more than drying treatment under 40-50 ℃ the operating mode, obtain loose granulous Wuyi biocontrol microorganisms solid substances; The quick XZG Rotatingandflashstreamingdrier that uses is produced by Changzhou development drying plant ltd.
Embodiment 2, utilize the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, and the Wuyiencin submerged fermentation is used for preparing in the antibiotic process of Wuyi bacterium; In fermentor tank, drop into earlier 10% substratum, steam goes out behind the assorted bacterium, introduces the Wuyiencin bacterial classification; Carry out deep fermentation; Temperature is controlled at 32 ℃, ferments to make 60 hours the time to contain the antibiotic fermented feed liquid of Wuyi bacterium, utilizes plate-and-frame filter press to squeeze out again and contains the antibiotic clear liquid of Wuyi bacterium in order to extracting the product of Wuyiencin; Plate-and-frame filter press is the XX type pressure filter that Jinan machine factory produces; The waste residue that squeezes out in the board and frame machine is pie more, and nutrition source, Wuyi bacterium mycelium, the sophisticated spore of Wuyi bacterium and a small amount of Wuyi bacterium exopolysaccharide that includes few not complete digestion is the bacterial classification source of production Wuyi biocontrol microorganisms.
The process method of Wuyi biocontrol microorganisms comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: send into aseptic proportioning room after the waste residue of above-mentioned plate-and-frame filter press extrusion was cleared up in 2 hours;
(2) solid-state fermentation culture medium procedure for preparation: get the Semen Maydis powder of the bean powder of relative waste residue weight 10%, relative waste residue weight 22%, the starch of relative waste residue weight 6% and the fish meal of relative waste residue weight 3%; Utilize high-pressure steam sterilizing pan to carry out the layering sterilization; Vapor pressure 0.3MPa, disinfecting time 50 minutes obtains solid-state fermentation culture medium; The high-pressure steam sterilizing pan model is a GT7C2000 type series horizontal sterilizer, and manufacturer is a Wuxi City food mechanical equipment factory;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium after will sterilizing and the waste residue cake that contains Wuyi biocontrol microorganisms bacterial classification; The mechanical stirring thorough mixing; Obtain solid-state cultivation material, use JMF-140 type stirrer, manufacturer is a Wuxi City food mechanical equipment factory;
(4) solid-state cultivation operation: the solid-state cultivation material that will stir evenly be laid in culture bed on, culture bed is the multilayer shelf, the gauge control of solid-state cultivation material is at 8 centimetres; Watering makes the water cut of solid-state cultivation material be controlled at 30-40%, and culture temperature is controlled at 30-45 ℃; Cultivate the material surface when being covered with a large amount of mycelia, will cultivate material and all turn over one time, so that the cultivation material is all accepted air and moisture up and down; So that mycelia enlarges increment on the surface, incubation time 65 hours obtains containing a large amount of Wuyis biocontrol microorganisms and mycelial solid substances; This Wuyi biocontrol microorganisms is measured its spore amount with blood platelet counting plate and is reached 40-50 hundred million/gram, promptly reaches training objective;
(5) drying and crushing operation: the solid fermentation late stage of culture, moisture content can drop to 20-30%, is than rarefaction, carries out drying and pulverization process easily.To reach the whole solid-state culture of cultivating the spore amount takes off from culture bed; Concentrate and send into the drying plant; Utilize the fast rotational flash dryer, with the solid fermentation thing blow floating, rotate, shearing, collision, friction and micronize, dry, pulverize, screening once accomplishes; Guaranteed that Wuyi biocontrol microorganisms and spore are being no more than drying treatment under 40-50 ℃ the operating mode; Obtain loose granulous Wuyi biocontrol microorganisms solid substances, the quick XZG Rotatingandflashstreamingdrier of use is produced by Changzhou development drying plant ltd.
Embodiment 3, utilize the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, and the Wuyiencin submerged fermentation is used for preparing in the antibiotic process of Wuyi bacterium; In fermentor tank, drop into earlier 10% substratum, steam goes out behind the assorted bacterium, introduces the Wuyiencin bacterial classification; Carry out deep fermentation; Temperature is controlled at 35 ℃, ferments to make 65 hours the time to contain the antibiotic fermented feed liquid of Wuyi bacterium, utilizes plate-and-frame filter press to squeeze out again and contains the antibiotic clear liquid of Wuyi bacterium in order to extracting the product of Wuyiencin; Plate-and-frame filter press is the XX type pressure filter that Jinan machine factory produces; The waste residue that squeezes out in the board and frame machine is pie more, and nutrition source, Wuyi bacterium mycelium, the sophisticated spore of Wuyi bacterium and a small amount of Wuyi bacterium exopolysaccharide that includes few not complete digestion is the bacterial classification source of production Wuyi biocontrol microorganisms.
The process method of Wuyi biocontrol microorganisms comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: send into aseptic proportioning room after the waste residue of above-mentioned plate-and-frame filter press extrusion was cleared up in 1 hour;
(2) solid-state fermentation culture medium procedure for preparation: get the Semen Maydis powder of the bean powder of relative waste residue weight 12%, relative waste residue weight 20%, the starch of relative waste residue weight 8% and the fish meal of relative waste residue weight 5%; Utilize high-pressure steam sterilizing pan to carry out the layering sterilization; Vapor pressure 0.3MPa, disinfecting time 45 minutes obtains solid-state fermentation culture medium; The high-pressure steam sterilizing pan model is a GT7C2000 type series horizontal sterilizer, and manufacturer is a Wuxi City food mechanical equipment factory;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium after will sterilizing and the waste residue cake that contains Wuyi biocontrol microorganisms bacterial classification; The mechanical stirring thorough mixing; Obtain solid-state cultivation material, use JMF-140 type stirrer, manufacturer is a Wuxi City food mechanical equipment factory;
(4) solid-state cultivation operation: the solid-state cultivation material that will stir evenly be laid in culture bed on, culture bed is the multilayer shelf, the gauge control of solid-state cultivation material is at 8 centimetres; Watering makes the water cut of solid-state cultivation material be controlled at 30-40%, and culture temperature is controlled at 30-45 ℃; Cultivate the material surface when being covered with a large amount of mycelia; To cultivate material and all turn over one time, all accept air and moisture up and down so that cultivate material, so that mycelia enlarges increment on the surface; Incubation time 60 hours; Obtain containing a large amount of Wuyis biocontrol microorganisms spore and mycelial solid substances, this Wuyi biocontrol microorganisms is measured its spore amount with blood platelet counting plate and is reached 40-50 hundred million/gram, promptly reaches training objective;
(5) drying and crushing operation: the solid fermentation late stage of culture, moisture content can drop to 20-30%, is than rarefaction, carries out drying and pulverization process easily.To reach the whole solid-state culture of cultivating the spore amount takes off from culture bed; Concentrate and send into the drying plant; Utilize the fast rotational flash dryer, with the solid fermentation thing blow floating, rotate, shearing, collision, friction and micronize, dry, pulverize, screening once accomplishes; Guaranteed that Wuyi biocontrol microorganisms and spore are being no more than drying treatment under 40-50 ℃ the operating mode; Obtain loose granulous Wuyi biocontrol microorganisms solid substances, the quick XZG Rotatingandflashstreamingdrier of use is produced by Changzhou development drying plant ltd.
Embodiment 4, utilize the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, and the Wuyiencin submerged fermentation is used for preparing in the antibiotic process of Wuyi bacterium; In fermentor tank, drop into earlier 10% substratum, steam goes out behind the assorted bacterium, introduces the Wuyiencin bacterial classification; Carry out deep fermentation; Temperature is controlled at 35 ℃, ferments to make 60 hours the time to contain the antibiotic fermented feed liquid of Wuyi bacterium, utilizes plate-and-frame filter press to squeeze out again and contains the antibiotic clear liquid of Wuyi bacterium in order to extracting the product of Wuyiencin; Plate-and-frame filter press is the XX type pressure filter that Jinan machine factory produces; The waste residue that squeezes out in the board and frame machine is pie more, and nutrition source, Wuyi bacterium mycelium, the sophisticated spore of Wuyi bacterium and a small amount of Wuyi bacterium exopolysaccharide that includes few not complete digestion is the bacterial classification source of production Wuyi biocontrol microorganisms.
The process method of Wuyi biocontrol microorganisms comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: send into aseptic proportioning room after the waste residue of above-mentioned plate-and-frame filter press extrusion was cleared up in 2 hours;
(2) solid-state fermentation culture medium procedure for preparation: get the Semen Maydis powder of the bean powder of relative waste residue weight 8%, relative waste residue weight 18%, the starch of relative waste residue weight 3% and the fish meal of relative waste residue weight 4%; Utilize high-pressure steam sterilizing pan to carry out the layering sterilization; Vapor pressure 0.3MPa, disinfecting time 48 minutes obtains solid-state fermentation culture medium; The high-pressure steam sterilizing pan model is a GT7C2000 type series horizontal sterilizer, and manufacturer is a Wuxi City food mechanical equipment factory;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium after will sterilizing and the waste residue cake that contains Wuyi biocontrol microorganisms bacterial classification; The mechanical stirring thorough mixing; Obtain solid-state cultivation material, use JMF-140 type stirrer, manufacturer is a Wuxi City food mechanical equipment factory;
(4) solid-state cultivation operation: the solid-state cultivation material that will stir evenly be laid in culture bed on, culture bed is the multilayer shelf, the gauge control of solid-state cultivation material is at 8 centimetres; Watering makes the water cut of solid-state cultivation material be controlled at 30-40%, and culture temperature is controlled at 30-45 ℃; Cultivate the material surface when being covered with a large amount of mycelia; To cultivate material and all turn over one time, all accept air and moisture up and down so that cultivate material, so that mycelia enlarges increment on the surface; Incubation time 60 hours; Obtain containing a large amount of Wuyis biocontrol microorganisms spore and mycelial solid substances, this Wuyi biocontrol microorganisms is measured its spore amount with blood platelet counting plate and is reached 40-50 hundred million/gram, promptly reaches training objective;
(5) drying and crushing operation: the solid fermentation late stage of culture, moisture content can drop to 20-30%, is than rarefaction, carries out drying and pulverization process easily.To reach the whole solid-state culture of cultivating the spore amount takes off from culture bed; Concentrate and send into the drying plant; Utilize the fast rotational flash dryer, with the solid fermentation thing blow floating, rotate, shearing, collision, friction and micronize, dry, pulverize, screening once accomplishes; Guaranteed that Wuyi biocontrol microorganisms and spore are being no more than drying treatment under 40-50 ℃ the operating mode; Obtain loose granulous Wuyi biocontrol microorganisms solid substances, the quick XZG Rotatingandflashstreamingdrier of use is produced by Changzhou development drying plant ltd.
In the drying and crushing operation (5) of above embodiment, can also adopt following method: the solid fermentation late stage of culture, moisture content can drop to 20-30%, is than rarefaction, carries out drying and pulverization process easily.To reach the whole solid-state culture of cultivating the spore amount and take off, and concentrate and send into the drying plant, carry out drying treatment, carry out pulverization process with kibbler then, obtain Wuyi biocontrol microorganisms solid substances with Vacuumdrier from culture bed.
The plate-and-frame filter press that uses among the above embodiment, high-pressure steam sterilizing pan, stirrer, Rotatingandflashstreamingdrier, Vacuumdrier and kibbler are common equipment.
One, the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to cucumber and makes an experiment, this test is carried out in green cucumber district, dyke strengthening village, Weifang City, Shandong Province, and the plantation level of management are consistent.
Test group and control group are established in test, and test group adopts the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, and control group adopts the amino acid water-soluble fertilizer.Test being provided with 5 plantation sub-districts, and wherein the Wuyi biocontrol microorganisms that embodiment 1 makes is adopted in 1 sub-district, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 2 to make; 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 3 to make, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 4 to make, the amino acid water-soluble fertilizer is adopted in other 1 sub-district; The sub-district random alignment; Put down in writing the output of each sub-district, obtain per mu yield and raising the output per-cent, the result of test group and control group is compared.The Wuyi biocontrol microorganisms adopts 50 times of liquid ditches to apply and spreads manuer in holes, 5 kilograms of every mu of consumptions, and 10 days are once, use 3 times.
Adopt Wuyi biocontrol microorganisms and the amino acid water-soluble fertilizer of 4 embodiment of the present invention, the contrast of cucumber yield such as following table:
Can find out from above data; The Wuyi biocontrol microorganisms of 4 embodiment of the present invention and present general amino acid water-soluble fertilizer are applied to the production of cucumber; The units increased in production of the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is higher; In the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, the effect of increasing production of embodiment 3 is best.
Two, the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to the induction of resistance experiment of plant; This test Que Mianhuazhongzhiqu before the Hanting District of Weifang, Shandong Province carries out; This growing area cotton wilt is more serious; Choosing disease index is 6.2, and symptom is that cotyledon or true leaf appearance cotton yellow or faint yellow patch prevents and treats test.
Test group and control group are established in test, and test group adopts the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, and control group adopts derosal to spray.Choose the growing area of 5 state of an illness basically identicals, wherein the Wuyi biocontrol microorganisms that embodiment 1 makes is adopted in 1 sub-district, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 2 to make; 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 3 to make; 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 4 to make, derosal, sub-district random alignment are adopted in other 1 sub-district; The scab of adding up each sub-district suppresses situation; Put down in writing the output of each sub-district, obtain increasing production per-cent, the result of test group and control group is compared.The Wuyi biocontrol microorganisms adopts 50 times of liquid ditches to apply and spreads manuer in holes, 5 kilograms of every mu of consumptions, and 10 days are once, use 3 times.
Adopt the Wuyi biocontrol microorganisms of 4 embodiment of the present invention to prevent and treat comparing result such as following table with adopting derosal:
Can find out from above data; The Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to prevent and treat cotton wilt with present general derosal; The control of the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is better; In the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, the control effect of embodiment 3 is best, and the effect of increasing production of embodiment 3 is best.
Three, the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to the induction of resistance experiment of plant; This test is carried out in pepper planting district, rice field, Shouguang, Weifang, Shandong Province; This growing area capsicum damping-off is more serious; Choosing disease index is 5.5, and symptom is prevented and treated test for the capsicum that the seedling basal part of stem produces oval dun scab.
Test group and control group are established in test, and test group adopts the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, and control group adopts the first holder to spray.Choose the growing area of 5 state of an illness basically identicals, wherein the Wuyi biocontrol microorganisms that embodiment 1 makes is adopted in 1 sub-district, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 2 to make; 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 3 to make, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 4 to make, the first holder is adopted in other 1 sub-district; The sub-district random alignment, the scab of adding up each sub-district suppresses situation, puts down in writing the output of each sub-district; Obtain increasing production per-cent, the result of test group and control group is compared.The Wuyi biocontrol microorganisms adopts 50 times of liquid ditches to apply and spreads manuer in holes, 5 kilograms of every mu of consumptions, and 10 days are once, use 3 times.
Adopt the Wuyi biocontrol microorganisms of 4 embodiment of the present invention to prevent and treat comparing result such as following table with adopting the first holder:
Can find out from above data; The Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to prevent and treat the capsicum damping-off with general first holder at present; The control of the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is better; In the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, the control effect of embodiment 2 is best, and the effect of increasing production of embodiment 2 is best.
Four, the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to the induction of resistance experiment of plant; Fangzi District chaste tree mountain depression planting asparagus district carries out in Weifang, Shandong Province in this test; This growing area asparagus stem wilt is more serious; Choosing disease index is 6.8, and symptom is that the asparagus that occurs long fusiform or oval dun color spot on the stem prevents and treats test.
Test group and control group are established in test, and test group adopts the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, and control group adopts the first holder to spray.Choose the growing area of 5 state of an illness basically identicals, wherein the Wuyi biocontrol microorganisms that embodiment 1 makes is adopted in 1 sub-district, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 2 to make; 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 3 to make, 1 Wuyi biocontrol microorganisms that the sub-district adopts embodiment 4 to make, the first holder is adopted in other 1 sub-district; The sub-district random alignment, the scab of adding up each sub-district suppresses situation, puts down in writing the output of each sub-district; Obtain increasing production per-cent, the result of test group and control group is compared.The Wuyi biocontrol microorganisms adopts 50 times of liquid ditches to apply and spreads manuer in holes, 5 kilograms of every mu of consumptions, and 10 days are once, use 3 times.
Adopt the Wuyi biocontrol microorganisms of 4 embodiment of the present invention to prevent and treat comparing result such as following table with adopting the first holder:
Can find out from above data; The Wuyi biocontrol microorganisms of 4 embodiment of the present invention is applied to prevent and treat the asparagus stem wilt with general first holder at present; The control of the Wuyi biocontrol microorganisms of 4 embodiment of the present invention is better; In the Wuyi biocontrol microorganisms of 4 embodiment of the present invention, the control effect of embodiment 1 is best, and the effect of increasing production of embodiment 1 is best.
Claims (4)
1. utilize the process method of the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation, it is characterized in that: said process method comprises maintenance bacterial classification spore operation, solid-state fermentation culture medium procedure for preparation, introduces a fine variety proportion mixed process and solid-state cultivation operation;
(1) keeps bacterial classification spore operation: get the waste residue that the Wuyiencin submerged fermentation produces;
(2) solid-state fermentation culture medium procedure for preparation: the bean powder that to get relative waste residue weight be 8-12%, relatively waste residue weight be 18-32% Semen Maydis powder, waste residue weight is the starch of 3-8% and the fish meal that relative waste residue weight is 3-5% relatively; Carry out the steam sterilizing sterilization, obtain solid-state fermentation culture medium;
(3) introduce a fine variety proportion mixed process: solid-state fermentation culture medium and waste residue after will sterilizing mix, and obtain solid-state cultivation material;
(4) solid-state cultivation operation: with solid-state cultivation material be laid in culture bed on, the water cut of cultivating material is controlled at 30-40%, temperature is controlled at 30-45 ℃, obtains containing the solid-state cultivation material of Wuyi biocontrol microorganisms spore, spore content is 40-50 hundred million/gram.
2. the process method of utilizing the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation as claimed in claim 1, it is characterized in that: said process method also comprises:
(5) drying and crushing operation: solid-state cultivation material is taken off from culture bed, carry out drying, pulverization process, obtain the solid-state cultivation material of granular Wuyi biocontrol microorganisms.
3. the process method of utilizing the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation as claimed in claim 1 is characterized in that: in the said maintenance bacterial classification spore operation, waste residue was sent into aseptic proportioning room in 2 hours.
4. the process method of utilizing the waste residue production Wuyi biocontrol microorganisms of Wuyiencin submerged fermentation as claimed in claim 1, it is characterized in that: in the said solid-state fermentation culture medium procedure for preparation, vapor pressure is 0.3MPa, and the steam sterilizing disinfecting time is 40-60 minute.
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| CN102533630B (en) * | 2011-11-09 | 2013-06-05 | 潍坊万胜生物农药有限公司 | Wuyiencin spore powder and production technology thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1620871A (en) * | 2004-10-22 | 2005-06-01 | 潍坊万胜生物农药有限公司 | Wuyiencin bactericide extraction and concentration method |
| CN101050441A (en) * | 2007-03-30 | 2007-10-10 | 中国农业科学院植物保护研究所 | Heavy producing bacterial strain F64 for Wuyi bacterin, and fermentation process |
| CN101066059A (en) * | 2007-06-04 | 2007-11-07 | 中国农业科学院植物保护研究所 | Soluble Wuyiencin powder and its prepn process |
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| CN1620871A (en) * | 2004-10-22 | 2005-06-01 | 潍坊万胜生物农药有限公司 | Wuyiencin bactericide extraction and concentration method |
| CN101050441A (en) * | 2007-03-30 | 2007-10-10 | 中国农业科学院植物保护研究所 | Heavy producing bacterial strain F64 for Wuyi bacterin, and fermentation process |
| CN101066059A (en) * | 2007-06-04 | 2007-11-07 | 中国农业科学院植物保护研究所 | Soluble Wuyiencin powder and its prepn process |
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