CN101948729B - Deer turtle ginseng wine and preparation method thereof - Google Patents
Deer turtle ginseng wine and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a deer turtle ginseng wine and a preparation method thereof. The deer turtle ginseng wine is characterized in that the wine uses the sinew of red deer, the bone of red deer, turtle shell glue, ginseng, astragalus root, Rhizoma polygonati, cinnamon, Chinese wolfberry, yam, white wine and purified water as raw materials, is a health food prepared by main working procedures of leaching, mixing, blending, standing, filtering, filling and the like and has the health functions of increasing immunity and relieving fatigue. The wine and the preparation method thereof have the following advantages: the proportions are reasonable, the raw materials are simple and not complicated, tonification and purgation are combined, the synergy is obvious; and the flavor is unique, the wine is convenient to drink and carry, the production control is easy and the wide extremely has market development potential.
Description
Technical field
The present invention relates to a kind of deer tortoise ginseng wine and preparation method thereof, a kind of deer tortoise ginseng wine with obvious strengthening immunity and alleviating physical fatigue function of more specifically saying so belongs to nutritious tonifying wine and health food technology field.
Background technology
Through the ages, health is the human eternal theme of pursuing always.The middle-aged people of today; Be the pillar of family, the backbone of unit; And the quickening of rhythm of life, WP add these middle-aged people's mental and physical efforts of ambassador and muscle power tired out; Exist the subhealth state phenomenon more or less, confirmed fatigue occurs and exempt from the low symptom of tired power, mainly show as be prone to catch a cold, symptoms such as insomnia and dreamful sleep, soreness of the waist and knees, sensation of oppression over the chest with shortness of breath, forgetful, alopecia.The swift and violent increase of sub-health population makes antifatigue and the protective foods increase in demand that improves immunizing power.
Utilize Chinese herbal medicine soaking to blend out health-care tonic wine, this is China working people and the greatly invention of successive dynasties medical worker in the long-term process of struggling with disease, is the rarity in the Chinese traditional culture.No matter pass by with now, health-care tonic wine all plays an important role in China people and the people of the world's culture life and health care.But health-care tonic wine mostly is to add the direct infusion of liquor with raw medicinal herbs to form in the prior art; This preparing method's limitation is very big; The one, the compatibility of medicinal material and consumption be difficult for to be grasped, and function singleness (being main with establishing-Yang mostly), health-care effect are not obvious, even some spinoffs are arranged; The 2nd, infusion is more bothersome, and many effective constituents are not fully utilized, and preparation cost is higher; The 3rd, mouthfeel also is difficult to accepted by people, and both inconvenience was preserved, and was also not portable; The 4th, sanitary condition is not ideal, and quality also is not easy control.
Summary of the invention
To the problem of above-mentioned prior art existence, the long-pending experience of being engaged in this area work for many years of the inventor, research and demonstration testing get generation of the present invention eventually repeatedly.
The object of the invention just is to provide a kind of deer tortoise ginseng wine and preparation method thereof; Remarkable strengthening immunity after particularly a kind of the drinking regains one's strength, with Ginseng Extract fast; Make people's energy more vigorous, comprehensive deer tortoise ginseng wine that keeps fit and preparation method thereof.
Main technical schemes of the present invention; Be through careful investigation and scrupulous comprehensively research; With reference to spreading the prescription composition of century-old strong health promoting wine among the people in grassland, Erdos, the Inner Mongol; And carry out scientific composition according to theory of traditional Chinese medical science, and confirm the prescription and the production technique of product through test of many times, each raw materials in part by weight all has synergistic function preferably in following ranges.
Technical solution of the present invention is: by red deer muscle, Cervus elaphus Linnaeas bone, Glue of Tortoise Plastron, genseng, the Radix Astragali, sealwort, Chinese cassia tree, wolfberry fruit, Chinese yam, liquor, purified water is that main raw material is processed, and wherein calculates with parts by weight: red deer muscle 0.4-0.8 part; Cervus elaphus Linnaeas bone 1-1.5 part; Glue of Tortoise Plastron 0.1-0.5 part; Genseng 0.5-0.6 part; Radix Astragali 0.5-0.6 part; Sealwort 0.5-0.6 part; Chinese cassia tree 0.3-0.6 part; Wolfberry fruit 0.5-0.7 part; Chinese yam 0.5-0.7 part; Liquor 60-70 part, all the other are the parts by weight of purified water, the gross weight umber is 100 parts.
In the technical scheme of the present invention; The quality standard of red deer muscle, Cervus elaphus Linnaeas bone meets the regulation of " first of the Sanitation Ministry medicine standard Chinese medicinal materials " version in 1992; The quality standard of Glue of Tortoise Plastron, genseng, the Radix Astragali, sealwort, Chinese cassia tree, wolfberry fruit, Chinese yam meets the regulation of " Beijing's prepared slices of Chinese crude drugs standard " version in 2000; The quality standard of liquor meets the hygienic standard of GB2757-1981 liquor and assembled alcoholic drinks, and the purifying quality standards for water meets the regulation of the Pharmacopoeia of the People's Republic of China 2000 version (two ones).
In addition, liquor according to the invention is the liquor of 60 degree.
Product forms of the present invention is a vina, because the concentration of liquor is different, the soluble end of effective constituent in the medicinal material is also had difference, and several kinds of raw material extraction process highway route design are for down in the present invention's prescription:
1., genseng, the Radix Astragali, sealwort, Chinese cassia tree group:
The main functional component of genseng is ginsenoside, polysaccharide etc. in this group.The main functional component of the Radix Astragali is triterpenoid saponin, flavones and polysaccharide etc.Sealwort: staple is saponin(e, polysaccharide, nicotinic acid, quinones, amino acid, trace element etc.Chinese cassia tree, staple are volatile oil, cinnamic aldehyde, small amount of acetic acid cassia bark ester, phenylpropyl acetate etc.We find that in orthogonal test research the consumption of liquor has the significance influence to the content of total saponin, from convenient for production simple, be easy to control and energy-saving and cost-reducing angle and see, preferred liquor consumption is 8 times, and soak time 40 days, liquor concentration are 60 degree,
2., wolfberry fruit, Chinese yam group:
The wolfberry fruit staple is a polysaccharide in this group, compositions such as β-Hu Luobusu, vegeto-alkali (trimethyl-glycine), plant pigments, VITAMINs, trace element, amino acid.The Chinese yam staple is saponin(e, phlegmatic temperament, choline, VC, glycan, starch etc.More than the design of two kinds of Chinese medicines both can remove macromole impurity with lower concentration 40 degree soaking in Chinese liquor extractions, and the unlikely loss of polysaccharide is too much, can meet the requirement of vina solvent again.We find that in orthogonal test research the liquor consumption is 8 times of amounts, and liquor concentration is 40 degree, and soak time is 40, and the content of Crude polysaccharides is for the highest in the liquid concentrator.
3., red deer muscle, Cervus elaphus Linnaeas bone group:
The two all contains collagen protein and partial hydrolysate amino acid thereof etc., still contains compositions such as calcium, phosphorus in addition, traditional usage for steeping in wine bubble, poach, fry in shallow oil soup and form such as take; It is slow to have a stripping with steeping in wine bubble; Shortcomings such as the stripping utilization ratio is low, and the time is longer, and large-scale production efficient is low.The color and luster of health promoting wine, mouthfeel are generally extremely important concerning being suitable for the crowd, and mouthfeel is mellow, frankness, and medicine is fragrant, aroma is harmonious, is easy to the human consumer and accepts.Different production technology tends to exist fishy smell, different assorted flavor etc., is unfavorable for the formation of fragrance, is difficult near taste used in everyday.In addition, the stability of health promoting wine also is an important problem, and different extraction process parameters tends to cause that finished product is muddy and produces deposition.The present invention selects decocting cooking method extraction process for use, and we find that in orthogonal test research amount of water is 8 times of amounts, and decocting number of times is 3 times, and each decocting time is 3h, and total nitrogen content is the highest in the sensory evaluation score of liquid concentrator and the liquid concentrator.
4., Glue of Tortoise Plastron: mainly contain compositions such as collagen protein and polypeptide, amino acid, the present invention selects for use with the water logging bubble and makes its water-swelling according to its characteristic, after the heating for dissolving, directly adds technology.
The preparation method of deer tortoise ginseng wine of the present invention is:
1, the examination of supplementary material
Select for use fine grain liquor to make the wine base, Chinese medicinal materials is selected medicine materical crude slice for use, and red deer muscle, Cervus elaphus Linnaeas bone are selected dry product for use, and all raw materials conform to quality requirements and can go into operation through check.
2, lixiviate
2.1, with genseng, the Radix Astragali, sealwort, Chinese cassia tree with the airtight immersion of 8 times of amounts, 60 degree liquor 40 days, stir every day 1 time, each 30 minutes, soaking wine liquid is crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor, with wine-filtering fluid merge wine dipper liquid 1, subsequent use.
2.2, with the liquor of part 60 degree as required degree of falling to 40 degree, subsequent use.
2.3, with Chinese yam, wolfberry fruit with the airtight immersion of 8 times of amounts, 40 degree liquor 40 days, stir every day 1 time, each 30 minutes, wine liquid was crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 2, subsequent use.
3, water is carried
3.1 red deer muscle, Cervus elaphus Linnaeas bone are added pure water to be carried 3 times; Add 8 times of amount pure water heated and boiled 3 hours at every turn, cross merging filtrate behind 40 eye mesh screens, adopt the degreasing of filtrating of disc-type oil-water separator; Make the filtrating fat content below 0.1%; Need not add processing aid, avoid auxiliary agent residual, guarantee that raw material is natural pure.Concentrate (0.08M, pa80 ℃) to specific density 1.10 (surveying for 60 ℃) and get Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, subsequent use.
Soaked 12 hours 3.2 Glue of Tortoise Plastron is added 3 times of amount purified water, treat fully to soak bloated post-heating (80 ℃-100 ℃) to whole macerates, 40 orders are crossed and filtered Glue of Tortoise Plastron filtrating, and are subsequent use.
4, mix
With wine dipper liquid 1, wine dipper liquid 2, Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, Glue of Tortoise Plastron filtrating merges stirring and evenly mixing.
5, allotment
With liquor and purified water constant volume, blend specified amount and required alcoholic strength (36 °～38 °).
6, check
Interventions Requested: Crude polysaccharides, alcoholic strength, total saponin.
7, leave standstill
Sealing and standing 6 months.
8, filter, check
Get supernatant, filter with diatomite filter, warp after the assay was approved can.
Interventions Requested: alcoholic strength, total solids.
9, can
10, check
Interventions Requested: one-hundred-percent inspection projects such as total saponin, Crude polysaccharides, alcoholic strength, methyl alcohol, lead, arsenic, manganese, phenyl-hexachloride, DDT, net content, total solids.
11, finished product is put in storage after the assay was approved.
Crucial production process of the present invention is carried out at the clean area that meets ten thousand grades of GMP30.
Deer tortoise ginseng wine of the present invention should satisfy alcoholic strength 36-38 degree, and every 100ml contains total saponin (in ginsenoside Re) more than or equal to 10mg, and contains Crude polysaccharides (in VISOSE) more than or equal to 5mg.
Performance below in conjunction with each raw material and effective ingredient is further described the present invention:
Organic conception is the characteristics of traditional Chinese medical science immunity, and Total tune is stable to be that human body is resisted disease, ensured healthy basic of vital movement, and each integral part of body, qi-blood-body fluid, internal organs channels and collaterals etc. are to form the effect that system of defense, performance are escorted jointly.Therefore, keep the whole machine balancing state of body, the immunologic derangement state does not take place in body, and health could be healthy.It is that change through function of five internal organs is coordinated situation each other produces that muscle power and mental fatigue produce, the traditional Chinese medical science think kidney controlling essence storage, give birth to marrow, fill brain, relevant with all cerebrations of people.The balance of kidney yin kidney yang be each internal organs root of YIN and YANG this, so during imbalance of YIN and YANG, can cause the imbalance of YIN and YANG of other internal organs, fatigue phenomenon will appear.Deficiency of kidney-essence can cause lassitude, dizzy tinnitus, soreness of the waist and knees, insomnia forgetfulness, absent minded, the inferior a series of problems of inefficiency, and this is the main reveal any symptoms of current sub-health population.The help class Chinese medicine that adopts in the present invention's prescription according to the traditional Chinese medical science " equilibrium between yin and yang, qi-blood tonifying " theory, can play the health-care effect of strengthening immunity and antifatigue fully.
Cervus elaphus Linnaeas bone, red deer muscle: deer is precious medicinal animal, and its whole body nearly all can be used as medicine, and has nourishing, strong effect.Use prevention of deer product and treatment disease, in the existing long history of China.The red deer muscle is the tendon of animal in deer family red deer four limbs.It is salty, warm in nature to distinguish the flavor of.Return liver, kidney channel.Function: invigorating the liver and kidney, strengthening the bones and tendons, dispelling cold and dampness.Cervus elaphus Linnaeas bone is the bone of animal in deer family red deer.It is sweet, warm in nature to distinguish the flavor of.Return liver, kidney channel.Function: strengthen muscles and bones, dispel the wind and remove temperature, promoting tissue regeneration and ulcer healing.Cure mainly a little less than the filter labor bone, rheumatic arthralgia rushes down dysentery, scrofula, sore.
Glue of Tortoise Plastron: " Chinese pharmacopoeia is gone through version and is recorded, and is rare Chinese medicine.Glue of Tortoise Plastron, nature and flavor are salty, sweet, cold; Return liver, kidney, the heart channel of Hang-Shaoyin; The effect of have enriching yin, nourish blood, stopping blooding.Be used for deficiency of Yin hectic fever, hectic fever due to yin night sweat, soreness of the waist and knees, the deficiency of blood is sallow, under the uterine bleeding band.Modern study shows that Glue of Tortoise Plastron can be regulated body function, and the mechanism that excitating organism self is regulated strengthens the homeostasis state.
Genseng: sweet, the little hardship of nature and flavor, flat; Return spleen, lung, the heart channel of Hang-Shaoyin; Efficacy effect reinforces vital energy, and multiple arteries and veins takes off admittedly, reinforces the spleen to benefit the lung, and promotes the production of body fluid, and calms the nerves.Be used for body void and desire to take off, the cold weak pulse of limb, insufficiency of the spleen food is few, and the deficiency syndrome of the lung is breathed with cough, and Tianjin wound is thirsty, and interior heat is quenched one's thirst, and prolonged illness void is thin, and eye is lost in palpitation with fear, and the impotence palace is cold; Heart failure, heart shock.Genseng can improve thinking and body mobility; Not only can strengthen neural excitation, also can strengthen its process of inhibition, make and suppress to be tending towards concentrated; Thereby differentiation makes excited fully and suppresses two kinds of nerve processes and is able to balance, improves people's the intelligence and the efficiency of muscle power.Utilize Radix Ginseng extract that mouse endurance is studied, experimental result is found the ability significant prolongation mouse swimming with a load attached to the body time, continues swimming time and on average prolongs 31.84% than control group, continues the swimming maximum duration and on average improves 7.93min; Power exhausts swimming time and on average prolongs 30.64% than control group, and power exhausts the swimming maximum duration and on average improves 7.27% than control group.Explain that genseng can improve endurance and motor capacity, reduce the nervus centralis fatigue reaction.
The Radix Astragali: nature and flavor are sweet, temperature; Return lung, the spleen channel; The efficacy effect invigorating QI to consolidate the body surface resistance, diuresis holder poison, apocenosis, expelling pus and promoting granulation.It is weak to be used for the deficiency of vital energy, anorexia and loose stool, and sinking of QI of middle-JIAO is rushed down prolapse of the anus for a long time, the uterine bleeding of having blood in stool, the exterior deficiency spontaneous perspiration, deficiency of vital energy oedema, ulcer is difficult bursts, and bursts for a long time and does not hold back, and flaccidity of limbs due to deficiency of blood is yellow, and interior heat is quenched one's thirst; Chronic nephritis proteinuria, mellitus.The Radix Astragali has obvious promoter action to the antibody systematic function of normal body.Can improve the serum IgG content of mouse; The clinical application Radix Astragali and the Radix Astragali are that main compound treatment observation shows; Insufficiency of the spleen patient's IgG level is raise; High level drops to normal range before making chronic hepatitis patient IgG by treatment, and secretory immunoglobulin (SIgA) content in the flu susceptible person nasal secretions is raise.Antifatigue experiment to mouse shows that ginseng stilbene mixture has the obvious anti-fatigue effect.Clinical experiment shows, ginseng stilbene mixture has recoverys that accelerate fatigue, and the enhancing myocardial contraction improves the effect of heart function, can make the urine protein behind the hard training on a declining curve.
Sealwort: nature and flavor are sweet, and are flat; Return spleen, lung, kidney channel; The efficacy effect boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney-nourishing.Be used for weakness of the spleen and the stomach, fatigue and asthenia, dry food is few, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, interior heat is quenched one's thirst.Sealwort is important medicinal plant, contains sibiricoside, nicotinic acid, carbohydrate, quinones, amino acid and trace element, has immune stimulating, enhancing immunity, delays senility, effect such as antiviral.
Chinese cassia tree: nature and flavor are hot, sweet, big heat; Return kidney, spleen, the heart, Liver Channel; It is supporing yang that efficacy effect is mended fire, let the fire back to its origin, dispersing cold for relieving pain, promoting blood circulation to restore menstrual flow.Be used for waist knee crymodynia, suffer from a deficiency of the kidney and do to breathe heavily, the deficiency of yang is dazzle the eyes, the hot eyes pharyngalgia, and trusted subordinate's crymodynia, the cold of insufficiency type is vomited and diarrhoea, cold hernia, the globefish that runs quickly is through closing dysmenorrhoea.Chinese cassia tree is the most frequently used Chinese medicine, also is one of best condiment in food-processing industry, catering and the home cooking simultaneously.Current research confirms; Chinese cassia tree has obvious enhancement to immune function of human body; But its mechanism of action is the propagation and the differentiation of enhances human body T lymphocyte and B cell, and strengthens its function, also can strengthen the killing ability of killer cell and the phagocytic function of MNP simultaneously.
Wolfberry fruit: nature and flavor are sweet, and are flat; Return liver, kidney channel; Efficacy effect is nourishing liver and kidney, replenishing vital essence to improve eyesight.It is thanks to smart to be used for consumptive disease, soreness of waist and knee joint, and dizzy tinnitus, interior heat is quenched one's thirst, and the deficiency of blood is sallow, and blurred vision is not clear.LBP in the matrimony vine can increase mouse muscle glycogen, liver starch margin significantly; Reach swimming back 90min, 150min serum lactic dehydrogenase (LDH) total activity before improving motion; Reduce blood urea nitrogen increment after the mouse strenuous exercise, accelerate the speed of knowing of motion back blood urea nitrogen.LBP can strengthen the mouse red blood cell immunologic function, and the inhibition of the hematid immunity function due to the CY is had tangible antagonistic action.
Chinese yam: nature and flavor are sweet, and are flat; Return spleen, lung, kidney channel; Efficacy effect tonifying spleen nourishing the stomach, the beneficial lung that promotes the production of body fluid, the puckery essence of kidney tonifying.It is few to be used for insufficiency of the spleen food, incessant chronic diarrhea, and the deficiency syndrome of the lung is breathed with cough, emission due to deficiency of the kidney, under the band, frequent micturition, abnormal heat is quenched one's thirst.Chinese yam polysaccharide is a kind of effective ingredient in the Chinese yam, and research shows, Chinese yam has functions such as immunostimulant, anti-ageing, reducing blood-fat and mutation.
Advantage of the present invention and beneficial effect are:
1. the invention belongs to health promoting wine; With wine is solvent; Lend the Xin Wenhang of wine the property with tying-in of loosing; Infuse goes out the vegeto-alkali in the herbal medicine effectively, glucoside, tannin, resin, volatile oil, organic acid, amaroid, carbohydrate, partial pigment etc., and with strong points, dosage concentrates, it is simple to take, be convenient to storage, easy to carry, stored for a long time not bad.
2. the present invention starts with from invigorating the spleen and replenishing QI, kidney-replenishing blood-nourishing, with the overall balance of mediator's body negative and positive of qi and blood, thereby reaches the effect of strengthening immunity, alleviating physical fatigue.
3. the aspects such as color and luster, mouthfeel and solubleness of deer tortoise ginseng wine of the present invention all do not have any detrimentally affect, and normal drinking can not brought harm and toxic side effect to health.Its production and promoting the use of not only helps people's health, plays a positive role to strengthening national health quality, has the market development potential, and is practical.
Below in conjunction with specific embodiment the present invention being further described, is limitation of the present invention but should not understand.
Specific embodiment
Embodiment 1:
1, the examination of supplementary material
Select for use fine grain liquor to make the wine base, Chinese medicinal materials is selected medicine materical crude slice for use, and red deer muscle, Cervus elaphus Linnaeas bone are selected dry product for use, and all raw materials conform to quality requirements and can go into operation through check.
2, lixiviate
2.1, with genseng 1.25kg, Radix Astragali 1.5kg, sealwort 1.5kg, Chinese cassia tree 1.5kg, with 8 times of amounts, 60 degree liquor (46kg) airtight immersions 40 days, stir every day 1 time; Each 30 minutes; Soaking wine liquid is crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 1, subsequent use.
2.2, with the liquor of part 60 degree as required degree of falling to 40 degree, subsequent use.
2.3, with Chinese yam 1.75kg, wolfberry fruit 1.75kg, with 8 times of amounts, 40 degree liquor (28kg) airtight immersions 40 days, stir every day 1 time, each 30 minutes, wine liquid was crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 2, subsequent use.
3, water is carried
3.1 with red deer muscle 1kg, Cervus elaphus Linnaeas bone 3.75kg, add pure water and carry 3 times, add 8 times of amount pure water (38kg) heated and boiled 3 hours at every turn; Cross merging filtrate behind 40 eye mesh screens; Adopt the degreasing of filtrating of disc-type oil-water separator, make the filtrating fat content, need not add processing aid below 0.1%.Concentrate (0.08Mpa, 80 ℃) to specific density 1.10 (surveying for 60 ℃) and get Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, subsequent use.
3.2, add 3 times of amount purified water (3.75kg) and soaked 12 hours with Glue of Tortoise Plastron 1.25kg, treat fully to soak bloated post-heating (80 ℃-100 ℃) to whole macerates, 40 orders are crossed and filtered Glue of Tortoise Plastron filtrating, and are subsequent use.
4, mix
With wine dipper liquid 1, wine dipper liquid 2, Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, Glue of Tortoise Plastron filtrating merges stirring and evenly mixing.
5, allotment
With 60 the degree liquor 80kg and purified water 70kg constant volumes, blend specified amount and required alcoholic strength (36 °～38 °).
6, check
Interventions Requested: Crude polysaccharides, alcoholic strength, total saponin.
7, leave standstill
Sealing and standing 6 months.
8, filter, check
Get supernatant, filter with diatomite filter, warp after the assay was approved can.
Interventions Requested: alcoholic strength, total solids.
9, can
10, check
Interventions Requested: one-hundred-percent inspection projects such as total saponin, Crude polysaccharides, alcoholic strength, methyl alcohol, lead, arsenic, manganese, phenyl-hexachloride, DDT, net content, total solids.
11, finished product is put in storage after the assay was approved.
The main test of present embodiment product and functional evaluation experimental study result are following:
The assay of the deer tortoise ginseng wine product that embodiment 1 makes sees the following form 1:
Table 1: assay
| Interventions Requested | Assay |
| Color and luster | Brown yellow transparent liquid |
| Flavour, smell | Has these article inherent smell, free from extraneous odour |
| Impurity | Do not have |
| Total saponins (in the ginsenoside Re), mg/100mL | 14.2 |
| Crude polysaccharides (in VISOSE), mg/100mL | 6.88 |
| Alcoholic strength (v/v), % | 37.3 |
| Methyl alcohol, g/100ml | 0.004 |
| Potato spirit, g/100ml | 0.023 |
| Total solids, g/100ml | 1.10 |
| Plumbous (in Pb) mg/L | ＜0.12 |
| Arsenic (in As) mg/L | ＜0.3 |
| Manganese (in Mn) mg/L | ＜0.2 |
| Phenyl-hexachloride, mg/L | ＜0.04 |
| DDT, mg/L | ＜0.04 |
We are according to Ministry of Health's " protective foods check and evaluation technique standard " 2003 editions; Protective foods function assessment assessment process and method of inspection standard, second section function assessment evaluation test method are carried out the strengthening immunity and the antifatigue effect evaluation of above-mentioned deer tortoise ginseng wine.
The deer tortoise ginseng wine that present embodiment 1 makes is recommended adult (body weight is in 60kg), 1 time/day; 50mL/ time; To recommend 1,10 and 30 times of adult's intake mouse stomach dosage is set day, make negative control group with 15% wine base, the continuous irrigation stomach is surveyed each item index after 30 days.Experimental result is following:
One, function of enhancing immunity experimental result
1. deer tortoise ginseng wine of the present invention is to the influence of mouse internal organs/body weight ratio
Table 2. deer tortoise ginseng of the present invention wine is to the influence of mouse internal organs/body weight ratio
Spleen/body weight ratio P
1Value: each experimental group and negative control group are relatively
Thymus gland/body weight ratio P
2Value: each experimental group and negative control group are relatively
Visible by table 2; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Through statistical procedures; Its spleen/body weight ratio and thymus gland/body weight ratio does not have between basic, normal, high dose groups and negative control group than significant difference (p＞0.05), and deer tortoise ginseng wine promptly of the present invention does not have influence to internal organs/body weight ratio of mouse.
2 deer tortoise ginseng wine of the present invention are to the mouse cell Immune Effects
2.1 deer tortoise ginseng wine of the present invention is to the influence of mouse body weight and delayed allergy (DTH)
Table 3. deer tortoise ginseng of the present invention wine is to the influence of mouse body weight and delayed allergy (DTH)
P
1Value: each experimental group and negative control group are relatively
P
2Value: each experimental group and negative control group be * P＜0.05 * * P＜0.01 relatively
Visible by table 3, through statistical procedures, per os gives deer tortoise ginseng wine of the present invention 30 days, and there was no significant difference (p＞0.05) is compared in each dose groups mouse weightening finish between basic, normal, high dose groups and negative control group; Its swelling degree of the paw compares there was no significant difference (p＞0.05) between low dose group and negative control group; Significant difference (p＜0.05) is relatively arranged between middle dose groups and negative control group; Highly significant property difference (p＜0.01) is relatively arranged between high dose group and negative control group, and promptly the deer tortoise ginseng wine of the present invention of middle and high dosage can strengthen the delayed allergy of mouse.
2.2 deer tortoise ginseng wine of the present invention is to the influence of ConA inductive mouse lymphocyte transformation experiment
Table 4. deer tortoise ginseng of the present invention wine is to the influence of ConA inductive mouse lymphocyte transformation experiment
The P value: each experimental group and negative control group are relatively
Visible by table 4; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Through statistical procedures; Its lymphocytic multiplication capacity compares there was no significant difference (p＞0.05) between basic, normal, high dose groups and negative control group, deer tortoise ginseng wine promptly of the present invention does not have influence to ConA inductive mouse lymphocyte conversion capability.
3 deer tortoise ginseng wine of the present invention are to the influence of humoral immunization
3.1 the influence of deer tortoise ginseng wine antagonist founder cell number of the present invention
Table 5. deer tortoise ginseng of the present invention wine is to the influence of mouse antibodies founder cell number
The P value: each experimental group and negative control group be * P＜0.05 relatively
Visible by table 5, per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days, and through statistical procedures, its antibody-producting cell number compares there was no significant difference (p＞0.05) between low, middle dose groups and negative control group; Significant difference (p＜0.05) is relatively arranged between high dose group and negative control group, and promptly the deer tortoise ginseng wine of the present invention of high dosage has tangible influence to the antibody-producting cell number of mouse.
3.2 deer tortoise ginseng wine of the present invention is to mouse half hemolysis value (HC
50) influence.
Table 6. deer tortoise ginseng of the present invention wine is to mouse half hemolysis value HC
50Influence
The p value: each experimental group and negative control group be * p＜0.05 relatively
Visible by table 6; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Through statistical procedures; Its half hemolysis value compares there was no significant difference (p＞0.05) between low, middle dose groups and negative control group, significant difference (p＜0.05) is relatively arranged between high dose group and negative control group, and promptly the deer tortoise ginseng wine of the present invention of high dosage can improve the mouse half hemolysis value.
4 deer tortoise ginseng wine of the present invention are to the influence of mouse monokaryon-macrophage phagocytic function
4.1 deer tortoise ginseng wine of the present invention is cleaned up the influence of function to mouse monokaryon-scavenger cell carbon
Table 7 deer tortoise ginseng of the present invention wine is cleaned up the influence of function to mouse monokaryon-scavenger cell carbon
The P value: each experimental group and negative control group be * p＜0.05 relatively
Visible by table 7; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Through statistical procedures; Its carbon is cleaned up function there was no significant difference (p＞0.05) relatively between low, middle dose groups and negative control group, and significant difference (p＜0.05) is relatively arranged between high dose group and negative control group, and promptly the deer tortoise ginseng wine of the present invention of the high dosage carbon that can improve mouse monokaryon-scavenger cell is cleaned up ability.
4.2 deer tortoise ginseng wine of the present invention is to the influence of mouse body weight and the erythrocytic ability of macrophage phagocytic flesh
Table 8. deer tortoise ginseng of the present invention wine is to the influence of mouse body weight and the erythrocytic ability of macrophage phagocytic flesh
p
1Value: each experimental group and negative control group weightening finish are relatively
p
2Value: each experimental group and negative control group phagocytic rate (%) are relatively
p
3Value: each experimental group and negative control group phagocytic index are relatively
Visible by table 8, through statistical procedures, per os gives deer tortoise ginseng wine of the present invention 30 days, and there was no significant difference (p＞0.05) is compared in each dose groups mouse weightening finish between basic, normal, high dose groups and negative control group; Its phagocytic rate relatively there are no significant between basic, normal, high dose groups and negative control group difference (p＞0.05); Its phagocytic index relatively there are no significant between basic, normal, high dose groups and negative control group difference (p＞0.05), deer tortoise ginseng wine promptly of the present invention is engulfed the erythrocytic ability of flesh to mouse macrophage is not had influence.
5 deer tortoise ginseng wine of the present invention are to the active influence of NK cells in mice
Table 9. deer tortoise ginseng of the present invention wine is to the active influence of NK cells in mice
The P value: each experimental group and negative control group are relatively
Visible by table 9; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Through statistical procedures, its NK cytoactive compares there was no significant difference (p＞0.05) between basic, normal, high dose groups and negative control group, and deer tortoise ginseng wine promptly of the present invention does not have influence to the NK cytoactive of mouse.
6. function of enhancing immunity experiment conclusion
The per os group is given the deer tortoise of the present invention ginseng wine 30 days of mouse various dose, can improve half hemolysis value, the mouse antibodies founder cell number of mouse, the delayed allergy that can strengthen mouse, the carbon that can improve mouse monokaryon-scavenger cell and clean up ability; Body weight gain, internal organs/body weight ratio, ConA inductive mouse spleen lymphocyte conversion capability, Turnover of Mouse Peritoneal Macrophages to mouse engulfed the erythrocytic ability of flesh, the NK cytoactive does not have influence.Judge that thus deer tortoise ginseng drinking utensils of the present invention has the function of enhancing immunity effect.
Two, alleviating physical fatigue function test result
1 deer tortoise ginseng wine of the present invention is to the influence of mouse swimming with a load attached to the body time and mouse weightening finish
Table 10. deer tortoise ginseng of the present invention wine is to the influence of mouse swimming with a load attached to the body time and mouse weightening finish
P: each experimental group and negative control group be * relatively: compare P＜0.05 with negative control group
Visible by table 10; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Each experimental mice weightening finish is compared there was no significant difference (P＞0.05) with negative control group; The high dose group swimming time is longer than negative control group, and difference has significance (P＜0.05), explains that deer tortoise ginseng wine of the present invention can prolong the mouse swimming with a load attached to the body time and the mouse weightening finish is not had influence.
2 deer tortoise ginseng wine of the present invention are to the influence of mice serum urea and mouse weightening finish
Table 11. deer tortoise ginseng of the present invention wine is to the influence of mouse swimming back serum urea and mouse weightening finish
P: each experimental group and negative control group be * relatively: compare P＜0.05 with negative control group
Visible by table 11; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Each experimental mice weightening finish is compared there was no significant difference (P＞0.05) with negative control group; Serum urea is lower than negative control group after the swimming of high dose group mouse, and with negative control group significant difference (P＜0.05) is arranged relatively, explains that deer tortoise ginseng drinking utensils of the present invention has the effect that suppresses the serum urea rising of mouse swimming back and the mouse weightening finish is not had influence.
3 deer tortoise ginseng wine of the present invention are to the influence of Mouse Liver glycogen content and mouse weightening finish
Table 12. deer tortoise ginseng of the present invention wine is to the influence of Mouse Liver glycogen content and mouse weightening finish
P: each experimental group and negative control group are relatively
Visible by table 12; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Each experimental mice weightening finish is compared there was no significant difference (P＞0.05) with negative control group; Each dose groups hepatic glycogen content is compared unknown significance difference (P＞0.05) with control group, explain that deer tortoise of the present invention ginseng wine do not see influence to liver starch deposit and mouse weightening finish.
4 deer tortoise ginseng wine of the present invention are to the influence of mouse blood lactic acid value and mouse weightening finish
Table 13. gives deer tortoise ginseng wine mouse swimming of the present invention forward and backward and three time point blood of rest 20min lactic acid value
Table 14. deer tortoise ginseng of the present invention wine is to the influence of three time point blood of mouse lactic acid TG-AUC and mouse weightening finish
Three time point blood lactic acid TG-AUC=5 * (blood lactic acid value before the swimming+3 * back 0 minute blood lactic acid value+2 * 20 fens blood lactic acid value of swimming back rest of swimming)
P: each experimental group and negative control group be * relatively: compare P＜0.05 with negative control group
Visible by table 14; Per os gives the deer tortoise of the present invention of mouse various dose and joined wine 30 days; Each experimental mice weightening finish is compared there was no significant difference (P＞0.05) with negative control group; High dose group mouse back three the time point blood lactic acid TG-AUCs of swimming decrease, and with negative control group significant difference (P＜0.05) are arranged relatively, the generation of blood lactic acid when deer tortoise ginseng wine of the present invention can reduce swimming is described but mouse weightening finish nothing is influenced.
5. alleviating physical fatigue function test conclusion
Per os gives mouse deer tortoise ginseng of the present invention wine, and continuous irrigation stomach 30d can prolong the mouse swimming with a load attached to the body time, and the generation of serum urea reduce the generation of blood lactic acid, but each dose groups is not seen influence to liver starch deposit and mouse body weight when reducing mouse swimming.Judge that thus deer tortoise ginseng drinking utensils of the present invention has the alleviating physical fatigue function.
Embodiment 2
The weight of each raw material is respectively red deer muscle 10kg; Cervus elaphus Linnaeas bone 25kg; Glue of Tortoise Plastron 2.5kg; Genseng 12.5kg; Radix Astragali 12.5kg; Sealwort 12.5kg; Chinese cassia tree 7.5kg; Wolfberry fruit 12.5kg; Chinese yam 12.5kg; 60 degree liquor 1500kg, purified water 750kg.Preparation technology is with embodiment 1.
Embodiment 3
The weight of each raw material is respectively red deer muscle 4kg; Cervus elaphus Linnaeas bone 7.5kg; Glue of Tortoise Plastron 2.5kg; Genseng 3kg; Radix Astragali 3kg; Sealwort 3kg; Chinese cassia tree 3kg; Wolfberry fruit 3.5kg; Chinese yam 3.5kg; 60 degree liquor 350kg, purified water 200kg.Preparation technology is with embodiment 1.
Claims (5)
1. a deer tortoise ginseng wine is characterized in that by red deer muscle, Cervus elaphus Linnaeas bone, Glue of Tortoise Plastron, genseng, the Radix Astragali, sealwort, Chinese cassia tree, wolfberry fruit, Chinese yam, liquor, purified water being that raw material is processed, and wherein calculates with parts by weight: red deer muscle 0.4-0.8 part; Cervus elaphus Linnaeas bone 1-1.5 part; Glue of Tortoise Plastron 0.1-0.5 part; Genseng 0.5-0.6 part; Radix Astragali 0.5-0.6 part; Sealwort 0.5-0.6 part; Chinese cassia tree 0.3-0.6 part; Wolfberry fruit 0.5-0.7 part; Chinese yam 0.5-0.7 part; Liquor 60-70 part, all the other are the parts by weight of purified water, the gross weight umber is 100 parts; Its preparation method is:
(1), the examination of supplementary material
Select for use fine grain liquor to make the wine base, Chinese medicinal materials is selected medicine materical crude slice for use, and red deer muscle, Cervus elaphus Linnaeas bone are selected dry product for use, and all raw materials conform to quality requirements and can go into operation through check;
(2), lixiviate
(2.1), with genseng, the Radix Astragali, sealwort, Chinese cassia tree with the airtight immersion of 8 times of amounts, 60 degree liquor 40 days, stir every day 1 time, each 30 minutes, soaking wine liquid is crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor, with wine-filtering fluid merge wine dipper liquid 1, subsequent use;
(2.2), with the liquor of part 60 degree as required degree of falling to 40 degree, subsequent use;
(2.3), with Chinese yam, wolfberry fruit with the airtight immersion of 8 times of amounts, 40 degree liquor 40 days, stir every day 1 time, each 30 minutes, wine liquid was crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 2, subsequent use;
(3), water is carried
(3.1) red deer muscle, Cervus elaphus Linnaeas bone are added pure water and carry 3 times, add 8 times of amount pure water heated and boiled 3 hours, excessively merging filtrate behind 40 eye mesh screens at every turn; Adopt the degreasing of filtrating of disc-type oil-water separator, make the filtrating fat content, need not add processing aid below 0.1%; Avoid auxiliary agent residual, guarantee that raw material is natural pure, at 0.08Mpa; Be concentrated into specific density 1.10 under 80 ℃ of conditions and get Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, wherein the condition determination of specific density is 60 ℃, and is subsequent use;
(3.2) Glue of Tortoise Plastron is added 3 times of amount purified water and soaked 12 hours, treat fully to soak post-heating to 80 ℃-100 ℃ of extremely whole macerates that expand, 40 orders are crossed and filtered Glue of Tortoise Plastron filtrating, and are subsequent use;
(4), mix
With wine dipper liquid 1, wine dipper liquid 2, Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, Glue of Tortoise Plastron filtrating merges stirring and evenly mixing;
(5), allotment
With liquor and purified water constant volume, blend specified amount and required alcoholic strength is 36 °～38 °;
(6), check
Interventions Requested: Crude polysaccharides, alcoholic strength, total saponin;
(7), leave standstill
Sealing and standing 6 months;
(8), filter, check
Get supernatant, filter, through after the assay was approved can with diatomite filter;
Interventions Requested: alcoholic strength, total solids;
(9), can
(10), check
Interventions Requested: total saponin, Crude polysaccharides, alcoholic strength, methyl alcohol, lead, arsenic, manganese, phenyl-hexachloride, DDT, net content, total solids;
(11), finished product is put in storage after the assay was approved.
2. a kind of deer tortoise ginseng wine as claimed in claim 1, it is characterized in that: said preparation method is:
(1), the examination of supplementary material
Select for use fine grain liquor to make the wine base, Chinese medicinal materials is selected medicine materical crude slice for use, and red deer muscle, Cervus elaphus Linnaeas bone are selected dry product for use, and all raw materials conform to quality requirements and can go into operation through check;
(2), lixiviate
(2.1), with genseng 1.25kg, Radix Astragali 1.5kg, sealwort 1.5kg, Chinese cassia tree 1.5kg, with 8 times of amounts, 60 degree liquor 46kg airtight immersions 40 days, stir every day 1 time; Each 30 minutes; Soaking wine liquid is crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 1, subsequent use;
(2.2), with the liquor of part 60 degree as required degree of falling to 40 degree, subsequent use;
(2.3), with Chinese yam 1.75kg, wolfberry fruit 1.75kg, with 8 times of amounts, 40 degree liquor 28kg airtight immersions 40 days, stir every day 1 time, each 30 minutes, wine liquid was crossed 40 eye mesh screens, the dregs of a decoction through squeeze pressed liquor and wine-filtering fluid merge wine dipper liquid 2, subsequent use;
(3), water is carried
(3.1), with red deer muscle 1kg, Cervus elaphus Linnaeas bone 3.75kg; Adding pure water carries 3 times; Add 8 times of amount pure water 38kg heated and boiled 3 hours at every turn, cross merging filtrate behind 40 eye mesh screens, adopt the degreasing of filtrating of disc-type oil-water separator; Make the filtrating fat content below 0.1%, need not add processing aid; At 0.08Mpa, be concentrated into specific density 1.10 under 80 ℃ of conditions and get Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, wherein the condition determination of specific density is 60 ℃, and is subsequent use;
(3.2), with Glue of Tortoise Plastron 1.25kg, add 3 times of amount purified water 3.75kg and soaked 12 hours, treat fully to soak post-heating to 80 ℃-100 ℃ to the whole macerates that expand, 40 orders are crossed and filtered Glue of Tortoise Plastron filtrating, and are subsequent use;
(4), mix
With wine dipper liquid 1, wine dipper liquid 2, Cervus elaphus Linnaeas bone, red deer muscle liquid concentrator, Glue of Tortoise Plastron filtrating merges stirring and evenly mixing;
(5), allotment
With 60 degree liquor 80kg and purified water 70kg constant volumes, blend specified amount and required alcoholic strength is 36 °～38 °;
(6), check
Interventions Requested: Crude polysaccharides, alcoholic strength, total saponin;
(7), leave standstill
Sealing and standing 6 months;
(8), filter, check
Get supernatant, filter, through after the assay was approved can with diatomite filter;
Interventions Requested: alcoholic strength, total solids;
(9), can
(10), check
Interventions Requested: total saponin, Crude polysaccharides, alcoholic strength, methyl alcohol, lead, arsenic, manganese, phenyl-hexachloride, DDT, net content, total solids;
(11), finished product is put in storage after the assay was approved.
3. a kind of deer tortoise ginseng wine as claimed in claim 1, it is characterized in that: the weight of each raw material is respectively red deer muscle 10kg; Cervus elaphus Linnaeas bone 25kg; Glue of Tortoise Plastron 2.5kg; Genseng 12.5kg; Radix Astragali 12.5kg; Sealwort 12.5kg; Chinese cassia tree 7.5kg; Wolfberry fruit 12.5kg; Chinese yam 12.5kg; Liquor 1500kg, purified water 750kg.
4. a kind of deer tortoise ginseng wine as claimed in claim 1, it is characterized in that: the weight of each raw material is respectively red deer muscle 4kg; Cervus elaphus Linnaeas bone 7.5kg; Glue of Tortoise Plastron 2.5kg; Genseng 3kg; Radix Astragali 3kg; Sealwort 3kg; Chinese cassia tree 3kg; Wolfberry fruit 3.5kg; Chinese yam 3.5kg; Liquor 350kg, purified water 200kg.
5. like each described a kind of deer tortoise ginseng wine of claim 1-4, it is characterized in that: alcoholic strength is the 36-38 degree, every 100ml in ginsenoside Re, contain total saponin more than or equal to 10mg, and in VISOSE, contain Crude polysaccharides more than or equal to 5mg.
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| CN102363744A (en) * | 2011-10-25 | 2012-02-29 | 张朝峰 | Deer bone-tendon wine and its manufacturing method |
| CN108641870A (en) * | 2018-05-30 | 2018-10-12 | 金花企业(集团)股份有限公司西安金花制药厂 | A kind of health liquor and preparation method thereof with strengthen immunity and anti-fatigue effect |
| CN110144273A (en) * | 2019-06-11 | 2019-08-20 | 山西强尔健科技有限公司 | Auxiliary material fermented pretreatment method used in alcoholic drink mixed with fruit juice |
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| CN1065275C (en) * | 1998-05-26 | 2001-05-02 | 倪颖 | Lubao wine and its preparation |
| CN1331499C (en) * | 2005-07-22 | 2007-08-15 | 高忠元 | Health-care medicated wines with reinforcing-kidney and nourishing yin and strong then yang function, and its prepn. method |
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Address after: 013750 Inner Mongolia Wulanchabu city Liangcheng County Daihai Street Town building wine source road Patentee after: Inner Mongolia Hongmao Chinese medicine Limited by Share Ltd Address before: 013750 Inner Mongolia Wulanchabu city Liangcheng County Daihai Street Town building wine source road Patentee before: Inner Mongolia Hongmao Pharmaceutical Co., Ltd. |