CN101946771B - Application of Saikosaponin A to preparing pesticide for resisting tobacco mosaic virus (TMV) - Google Patents
Application of Saikosaponin A to preparing pesticide for resisting tobacco mosaic virus (TMV) Download PDFInfo
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- CN101946771B CN101946771B CN 201010291073 CN201010291073A CN101946771B CN 101946771 B CN101946771 B CN 101946771B CN 201010291073 CN201010291073 CN 201010291073 CN 201010291073 A CN201010291073 A CN 201010291073A CN 101946771 B CN101946771 B CN 101946771B
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- tmv
- saikosaponin
- virus
- tobacco mosaic
- mosaic virus
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Abstract
The invention provides application of Saikosaponin A to preparing a pesticide for resisting tobacco mosaic virus (TMV). Test results prove that the Saikosaponin A can remarkably inhibit the initial infestation of TMV to a host cell, thereby reducing the chance of the virus entering the host cell for copying and proliferating to play a role of protecting the host cell. The Saikosaponin A has inhibition ratio to the infestation by the TMV, which is superior to 'virus killer' as an anti-virus pesticide released to the market.
Description
Technical field
The invention belongs to technical field of pharmaceuticals, particularly, relate to saikoside A as the application of the preparation of prevention and control tobacco mosaic.
Background technology
The mosaic disease that is caused by tobacco mosaic virus is the important disease of cured tobacco production, causes the underproduction even total crop failure.The current anti-virus formulation that adopts in the control of tobacco mosaic can not fundamentally reduce disease to producing the loss that causes.Recklessly the attending effectiveness of bavin for and induce sweat in, soothing the liver, rise sun.Its composition Hu bavin saponin(e is used for the treatment of chronic viral hepatitis.
Summary of the invention
The purpose of this invention is to provide the application of saikoside A in preparation resisting tobacco mosaic virus medicine, and the preparation of the resisting tobacco mosaic disease that contains saikoside A is provided, and compound method.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
The application of saikoside A shown in the following structural formula in preparation resisting tobacco mosaic virus medicine,
When the compounds of this invention is used as medicine, can directly use, perhaps use with the form of pharmaceutical composition.This pharmaceutical composition contains 0.1-99%, is preferably the compounds of this invention of 0.5-90%, and all the other are acceptable on the pharmacology, pharmaceutically suitable carrier and/or the excipient of and inertia nontoxic to humans and animals.Described pharmaceutical carrier or excipient are one or more solids, semisolid and liquid diluent, filler and pharmaceutical preparation assistant agent.Pharmaceutical composition of the present invention is used with the form of per weight dose.The method that adopts pharmacy and field of food to generally acknowledge is prepared into various formulations, such as liquid preparation (injection, supensoid agent, emulsion, solution, syrup etc.), solid pharmaceutical preparation (tablet, capsule, granule, electuary etc.), spray, aerosol etc.
Description of drawings:
Fig. 1 is the inhibition (its left half leaf is for only inoculating TMV, and right half leaf is compound treatment) that the compounds of this invention saikoside A infects TMV on Nicotiana glutinosa.
Embodiment:
Further specify essentiality content of the present invention below in conjunction with concrete instance, but content of the present invention is not limited to this.
Embodiment 1:
The withered spot method of Nicotiana glutinosa half leaf: select healthy suitable Nicotiana glutinosa, a night is placed in the darkroom.Every cigarette is selected 3 identical blades of horizontal level.With concentration be the TMV of 0.032 μ g/ μ l and the compound saikoside A solution of 50 μ g/ml (experiment with compound in 2006 available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute, be the drug inspection reference substance.Compound is dissolved into the mother liquor of 10mg/ml in 4 ℃ of preservations with DMSO (DMSO), face the time spent to be diluted to desired concn with sterile water.The final concentration 25 μ l/ml of DMSO, on experimental result without impact.) mix 15min after frictional inoculation in Nicotiana glutinosa blade right half part 100 μ l, the TMV100 μ l of blade left-half frictional inoculation equal concentrations is positive control, and is clean with aseptic water washing behind the 10min.To test the cigarette seedling puts into without the worm net cage and cultivates, the withered spot number order of half leaf about 72h statistics blade, and by formula calculate the inhibiting rate that compound infects TMV=[(positive control withered spot number-compound treatment withered spot number)/positive control withered spot number] * 100%.
Because Nicotiana glutinosa belongs to the withered spot host of TMV, can judge whether compound has suppressed first the infecting of virus by the blade of a virus inoculation relatively with through withered spot number amount and the withered spot size of compound treatment blade.Fig. 1 is the inhibition that compound saikoside A infects TMV on the Nicotiana glutinosa when 50 μ g/ml, and its left half leaf is for only inoculating TMV, and right half leaf is inoculated processing for virus after compound treatment.By part withered spot number order contrast about blade as can be known, compound saikoside A can significantly suppress TMV the first of host infected, and also further copies and value-added chance thereby reduce the cell entry host cell, plays the effect to host's protection.Table 1 for compound saikoside A when 50 μ g/ml and control drug " virus must the restrain " comparison of on Nicotiana glutinosa, TMV being infected inhibiting rate, by the result as can be known, the inhibiting rate that compound infects TMV is better than commercially available antiviral agricultural chemicals " virus must restrain ".
Table 1 the compounds of this invention saikoside A and virus must restrain
On Nicotiana glutinosa, TMV is infected the comparison of inhibiting rate
Claims (1)
1. saikoside A shown in the following structural formula is as the application of unique active component in preparation resisting tobacco mosaic virus medicine,
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010291073 CN101946771B (en) | 2010-09-26 | 2010-09-26 | Application of Saikosaponin A to preparing pesticide for resisting tobacco mosaic virus (TMV) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010291073 CN101946771B (en) | 2010-09-26 | 2010-09-26 | Application of Saikosaponin A to preparing pesticide for resisting tobacco mosaic virus (TMV) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101946771A CN101946771A (en) | 2011-01-19 |
| CN101946771B true CN101946771B (en) | 2013-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010291073 Active CN101946771B (en) | 2010-09-26 | 2010-09-26 | Application of Saikosaponin A to preparing pesticide for resisting tobacco mosaic virus (TMV) |
Country Status (1)
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102363767A (en) * | 2011-10-12 | 2012-02-29 | 北京弘润天源生物技术有限公司 | Neuron-like cells obtained by processing neural stem cells by use of saikoside alpha, development of kit thereof and composition for treating nervous system diseases |
| CN102550377A (en) * | 2011-11-21 | 2012-07-11 | 云南省烟草公司昆明市公司 | Method for preventing and controlling mosaic viruses in tobacco float seedlings |
| CN109042658A (en) * | 2018-07-18 | 2018-12-21 | 合肥百绿盛农业科技有限公司 | The application method and its application of plant growth regulator composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899333A (en) * | 2005-07-25 | 2007-01-24 | 郭爱华 | Bupleurum root extract, its preparing method and its use |
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2010
- 2010-09-26 CN CN 201010291073 patent/CN101946771B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899333A (en) * | 2005-07-25 | 2007-01-24 | 郭爱华 | Bupleurum root extract, its preparing method and its use |
Non-Patent Citations (3)
| Title |
|---|
| 樊兵等.植物源抗病毒活性物质的初步筛选.《西北农林科技大学学报》.2005,第33卷167-170. * |
| 沈建国等.药用植物提取物抗烟草花叶病毒活性的研究.《中草药》.2006,第37卷(第2期),259-261. |
| 药用植物提取物抗烟草花叶病毒活性的研究;沈建国等;《中草药》;20060228;第37卷(第2期);259-261 * |
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| CN101946771A (en) | 2011-01-19 |
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