CN101946770B - Application of 6-O-caffeoyl erigeroside in preparing tobacco mosaic virus resistant medicament - Google Patents
Application of 6-O-caffeoyl erigeroside in preparing tobacco mosaic virus resistant medicament Download PDFInfo
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- CN101946770B CN101946770B CN2010102910126A CN201010291012A CN101946770B CN 101946770 B CN101946770 B CN 101946770B CN 2010102910126 A CN2010102910126 A CN 2010102910126A CN 201010291012 A CN201010291012 A CN 201010291012A CN 101946770 B CN101946770 B CN 101946770B
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- tobacco mosaic
- mosaic virus
- erigeroside
- caffeoyl
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Abstract
The invention provides the application of 6-O-caffeoyl erigeroside in preparing a tobacco mosaic virus resistant medicament. According to an experiment result, the compound 6-O-caffeoyl erigeroside can be used for obviously inhibiting the primary infection of TMV (Tobacco Mosaic Virus) to a host, thereby reducing the chances for the virus to enter a host cell and to be further replicated and incremented, and playing a role of protecting the host. The inhibition ratio of the invention on the TMV is superior to that of a commercial antiviral pesticide of Bilken viruside.
Description
Technical field
The invention belongs to technical field of pharmaceuticals, particularly, relate to of the application of 6-O-coffee acyl bitter fleabane glycosides as the preparation of prevention and control tobacco mosaic.
Background technology
Plant virus is the one type of plant disease that is only second to fungi and bacterium, and harm is big and be difficult to control, and the title of " plant cancer " is arranged.Tobacco mosaic virus disease is one of main disease of cured tobacco production, and annual tobacco mosaic virus can make a large amount of underproduction in a lot of cigarettes district even total crop failure, brings massive losses for numerous tobacco growers.Most of Antiphytoviral preparations of selling in the market can only prevent the development of infecting and delaying of virus or mitigation symptoms; Plant produces the loss that causes in case morbidity is difficult to fundamentally reduce give; And composition is generally to be mixed by the crude extract that extracts in the plant and forms, and concrete antiviral mechanism is also indeterminate.Though commercially available chemosynthesis medicament specific chemical components and have the effect of reasonable inhibition virus replication; But when spraying concentration is high slightly, can produce toxic action to plant itself equally, so still can not give the problem of bringing of producing to solving tobacco mosaic virus.
Summary of the invention
The purpose of this invention is to provide the application of 6-O-coffee acyl bitter fleabane glycosides in preparation resisting tobacco mosaic virus medicine.And the preparation of preventing and treating the tobacco mosaic that tobacco mosaic virus causes that contains 6-O-coffee acyl bitter fleabane glycosides is provided, and the compound method of said preparation.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
The application of 6-O-coffee acyl bitter fleabane glycosides shown in the following structural formula in preparation resisting tobacco mosaic virus medicine
When The compounds of this invention is used as medicine, can directly use, perhaps use with the form of pharmaceutical composition.This pharmaceutical composition contains 0.1-99%, is preferably the The compounds of this invention of 0.5-90%, and all the other are acceptable on the pharmacology, the pharmaceutically suitable carrier and/or the excipient of and inertia nontoxic to humans and animals.Described pharmaceutical carrier or excipient are one or more solids, semisolid and liquid diluent, filler and pharmaceutical preparation assistant agent.Pharmaceutical composition of the present invention is used with the form of per weight dose.Adopt pharmacy and the generally acknowledged method of field of food be prepared into various formulations, like liquid preparation (injection, supensoid agent, emulsion, solution, syrup etc.), solid pharmaceutical preparation (tablet, capsule, granule, electuary etc.), spray, aerosol etc.
Description of drawings:
The inhibition effect that Fig. 1 infects TMV on Nicotiana glutinosa for The compounds of this invention 6-O-coffee acyl bitter fleabane glycosides (wherein left half leaf is for only inoculating TMV, and right half leaf is a compound treatment).
Embodiment:
Below in conjunction with accompanying drawing, further specify essentiality content of the present invention with concrete instance of the present invention, but content of the present invention is not limited thereto.
Embodiment 1:
The withered spot method of Nicotiana glutinosa half leaf: select healthy suitable Nicotiana glutinosa, a night is placed in the darkroom.Every cigarette is selected 3 identical blades of horizontal level.With concentration be the TMV of 0.032 μ g/ μ l and the compound 6-O-coffee acyl bitter fleabane glycosides solution of 50 μ g/ml (experiment with compound in 2006 available from Nat'l Pharmaceutical & Biological Products Control Institute, be the drug inspection reference substance.The mother liquor that compound is dissolved into 10mg/ml with the inferior maple of dimethyl (DMSO) is in 4 ℃ of preservations, faces the time spent to be diluted to desired concn with sterile water.The final concentration 25 μ l/ml of DMSO do not have influence to experimental result.) mix 15min after frictional inoculation in Nicotiana glutinosa blade right half part 100 μ l, the TMV100 μ l of blade left-half frictional inoculation equal concentrations is positive control, and is clean with aseptic water washing behind the 10min.To test the cigarette seedling and put into no worm net cage and cultivate, the withered spot number order of half leaf about 72h statistics blade, and computerized compound inhibiting rate=[(positive control withered spot number-compound treatment withered spot number)/positive control withered spot number] * 100% that TMV is infected by formula.
Because Nicotiana glutinosa belongs to the withered spot host of TMV, can judge whether compound has suppressed first the infecting of virus through the blade of a comparison virus inoculation with through the withered spot number amount and the withered spot size of compound 6-O-coffee acyl bitter fleabane glycosides processing blade.The inhibition effect that Fig. 1 infects TMV on the Nicotiana glutinosa when 50 μ g/ml for compound 6-O-coffee acyl bitter fleabane glycosides, wherein left half leaf is for only inoculating TMV, and right half leaf is inoculated processing for virus after compound treatment.Can know that by part withered spot number order contrast about blade compound 6-O-coffee acyl bitter fleabane glycosides can significantly suppress TMV the first of host infected, also further duplicate and value-added chance, play effect host's protection thereby reduce virus entering host cell.Table 1 for compound 6-O-coffee acyl bitter fleabane glycosides when 50 μ g/ml and control drug " virus must the restrain " comparison of on Nicotiana glutinosa, TMV being infected inhibiting rate; Can know that by the result inhibiting rate that compound 6-O-coffee acyl bitter fleabane glycosides infects TMV is superior to commercial anti viral pesticide " virus must restrain ".
Table 1 The compounds of this invention 6-O-coffee acyl bitter fleabane glycosides must restrain on Nicotiana glutinosa the comparison of TMV being infected inhibiting rate with virus
Claims (1)
1. the application of the 6-O-coffee acyl bitter fleabane glycosides shown in the following structural formula in preparation resisting tobacco mosaic virus medicine,
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102910126A CN101946770B (en) | 2010-09-26 | 2010-09-26 | Application of 6-O-caffeoyl erigeroside in preparing tobacco mosaic virus resistant medicament |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102910126A CN101946770B (en) | 2010-09-26 | 2010-09-26 | Application of 6-O-caffeoyl erigeroside in preparing tobacco mosaic virus resistant medicament |
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| Publication Number | Publication Date |
|---|---|
| CN101946770A CN101946770A (en) | 2011-01-19 |
| CN101946770B true CN101946770B (en) | 2012-11-07 |
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| CN2010102910126A Active CN101946770B (en) | 2010-09-26 | 2010-09-26 | Application of 6-O-caffeoyl erigeroside in preparing tobacco mosaic virus resistant medicament |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115843807B (en) * | 2023-03-02 | 2023-04-28 | 云南省农业科学院生物技术与种质资源研究所 | Application of swertia herb flavin compound |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252276A (en) * | 1998-10-27 | 2000-05-10 | 国家医药管理局上海医药工业研究院 | Medicament containing 1-(2'-gamma-pyrone)-6-caffacyl-B-D-pyranoglucoside and its preparation |
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- 2010-09-26 CN CN2010102910126A patent/CN101946770B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252276A (en) * | 1998-10-27 | 2000-05-10 | 国家医药管理局上海医药工业研究院 | Medicament containing 1-(2'-gamma-pyrone)-6-caffacyl-B-D-pyranoglucoside and its preparation |
Non-Patent Citations (4)
| Title |
|---|
| 多舌飞蓬甙的分离和结构;张印俊等,1;《药学学报》;19981128(第11期);全文 * |
| 张印俊等,1.多舌飞蓬甙的分离和结构.《药学学报》.1998,(第11期),全文. |
| 植物源抗病毒活性物质的初步筛选;樊兵等,1;《西北农林科技大学学报(自然科学版)》;19220809;第33卷;全文 * |
| 樊兵等,1.植物源抗病毒活性物质的初步筛选.《西北农林科技大学学报(自然科学版)》.1922,第33卷全文. |
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| CN101946770A (en) | 2011-01-19 |
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