CN101940113A - Method for improving lead resistance of endive by spraying microorganism on blade surface - Google Patents
Method for improving lead resistance of endive by spraying microorganism on blade surface Download PDFInfo
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- CN101940113A CN101940113A CN2010102171139A CN201010217113A CN101940113A CN 101940113 A CN101940113 A CN 101940113A CN 2010102171139 A CN2010102171139 A CN 2010102171139A CN 201010217113 A CN201010217113 A CN 201010217113A CN 101940113 A CN101940113 A CN 101940113A
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Abstract
The invention provides a method for improving lead resistance of endive by spraying microorganism on blade surface, relating to a method for improving soil. In the method, the microorganism is spherical Rhodopseudomonas spheroids SHII-7. The method comprises the following steps: anaerobic illumination culturing is carried out for 4-5 days to obtain bactericide having the concentration of 1-2*108 live bacteria/ml; before the endive is seeded, the bactericide is taken out to be diluted by 10 times for soaking seeds; and then, the bactericide is diluted by 20-500 times and sprayed on the blade surface during the period of endive growth. The invention can increase the contents of chlorophyll and carotenoid; the dry weight and the fresh weight of the endive as well as photosynthesis are all greatly improved; vanes are large and deep green; the contents of malonaldehyde, proline, POD enzyme and other resistance indexes are improved so that the endive can tolerate lead pollution in 1200mg Pb/kg soil, thereby effectively reducing phenomena of less root quantities, slow growth, weakness over ground and the like; and the increase production effect is obvious.
Description
Technical field
The present invention relates to a kind of method of improving soil, particularly relate to a kind of method the lead pollution of the short living microorganism opposing of corn sow thistle utilization soil.
Background technology
Plumbous solvability in soil is little, and the holdup time is long, is a kind of nondegradable environmental contaminants.China's soil lead pollution amount is big and wide, fast development along with expressway construction, a large amount of uses of doped fuel, urban sewage and garbage disposal are improper, the unreasonable discharging of " three wastes " that industrial production produced, the use of agricultural chemicals, weed killer herbicide and chemical fertilizer, and the reasons such as abandoned land that cause because of mining are on the rise the soil lead pollution.
Lead is not the indispensable element that animal and plant grow.The plumbous passive plant roots that enters, skin or blade and accumulation make hazard of plant.Pb is significant to the influence of plant root growth, reduces the mitotic rate of root cells, and the root amount reduces, and root cap expands, blackening, rotten, and plant shoot decomposing biological amount descends subsequently, and leaf chlorosis is obvious, and is withered, dead gradually when serious; Plumbous accumulation also directly influences the metabolism of cell, the destruction that causes the active oxygen metabolism enzyme system.High Pb concentration also makes seed germination rate and radicle length, epicotyl length reduce, even the radicle necrosis occur.What more paid close attention to is that lead can enter food chain, jeopardizes human beings'health.Lead is multisystem, many organs property and irreversibility to body injury, and it is even more serious with the harm of intelligence to children's health as the central nervous system poisonous substance.
The improvement of lead-contaminated soil and development and use are mainly comprised two aspects, the improvement of first lead-contaminated soil, it two is anti-plumbous hereditary capacities of fully excavating and utilizing plant, promptly screens the anti-plumbous related gene of anti-plumbous kind and clone.The present physico-chemical process remediating lead-contaminated soil that adopts, the expense costliness, destroy environment, cause secondary pollution easily, and often can not reach the real plumbous purpose of removing, the phytoremediation technology that occurred in nearly ten years makes soil lead be removed under the condition of not destroying environment, is a kind of green environmental protection technique.The plant that is used for the rehabilitating soil lead pollution comprises roundleaf penny cress, cattail, sunflower, goldenrod, fescue grass, corn, corn sow thistle, Sedum alfredii Hance etc., and wherein economic crops form with the creation economic worth because of biomass is relatively large and are first-selection.But, no matter in the higher contaminated soil of lead concentration, carry out phytoremediation, still the plumbous economic crops of plantation tolerance are to obtain output in the lower soil of lead concentration, and plant strain growth is slow, growing way is weak, biomass is little all is one of the main factor of restriction effect.If can explore effective processing method, make the growth that crop still can be quick, a large amount of in lead-contaminated soil, then increase income all of great advantage for pollution control and crop.
Corn sow thistle is the wild leaf vegetables that is distributed widely in China northern territory, in recent years, having because of it to have in health-care efficacy such as refreshing and detoxicating and the hardship has fragrant extremely people's favor of unique smell in sweet, sweet, becomes indispensable delicacies on the spring and summer dining table, the large tracts of land artificial cultivation.Corn sow thistle is plumbous enriching plant, can remove lead in the soil by the mode of plant extraction, yet plumbous toxic action makes corn sow thistle root amount few, poor growth, and biomass is little.If can improve the anti-plumbous ability of corn sow thistle, when making it to keep higher concentration coefficient, increase biomass, then increased the extraction total amount of soil lead, accelerate the improvement process, and can gather in the crops higher output, create economy and social value.
Summary of the invention
The object of the present invention is to provide a kind of method to the lead pollution of the short living microorganism opposing of corn sow thistle utilization soil.This method has efficient promotion to corn sow thistle growth in the lead-contaminated soil, increases chlorophyll, carotenoid content, and it is few effectively to alleviate the root amount that causes because of saturnism, poor growth, phenomenon such as acrial part is small and weak, obvious effect of increasing production.
The objective of the invention is to be achieved through the following technical solutions:
The foliage-spray microorganism is improved the method for the anti-lead of corn sow thistle, and this method microorganism is spherical Rhodopseudomonas (Rhodopseudomonas spheroides) SH II-7, anaerobism illumination cultivation 4-5 days, and acquisition concentration is 1-2 * 10
8The microbial inoculum of individual viable bacteria/ml before the corn sow thistle sowing, is got 10 times of seed soaking of microbial inoculum dilution, and 200-500 times of microbial inoculum dilution after this is at corn sow thistle growth period foliage-spray.
Described foliage-spray microorganism is improved the method for the anti-lead of corn sow thistle, and the medium of its fungicide preparation is: NH
4Cl 1.0g, CaCl
20.02g, MgSO
47H
2O 0.2g, NaHCO
33.0g, K
2HPO
40.4g, NaCl 1.0g, peptone 1.0g, water 1000ml, PH neutrality was sterilized 30 minutes for 121 ℃, anaerobism illumination cultivation 4-5 days, acquisition concentration is 1-2 * 10
8The microbial inoculum of individual viable bacteria/ml.
Described foliage-spray microorganism is improved the method for the anti-lead of corn sow thistle, and its microbial inoculum application process is: before the corn sow thistle sowing, get 10 times of seed soaking of microbial inoculum dilution, after this microbial inoculum dilution 200-500 doubly, from two leaf phases, every 2-3 week foliage-spray 1 time is until the end of gathering; Or plant rises when just having shown poor growth, and foliage-spray is 1 time weekly, sprays 2 times continuously and stops.
Advantage of the present invention and effect are:
This microbial inoculum using method is easy, and production cost is low, and production process safety is used the back environmental friendliness, and corn sow thistle growth in the lead-contaminated soil is had efficient promotion.Extremely significantly increase chlorophyll, carotenoid content, dry weight, fresh weight, photosynthesis all increase substantially, blade hypertrophy, dark green, resistance index content such as malonaldehyde, proline, POD enzyme improve, make corn sow thistle can tolerate the lead-contaminated soil of 1200mgPb/kg soil, it is few effectively to alleviate the root amount that causes because of saturnism, poor growth, phenomenons such as acrial part is small and weak, obvious effect of increasing production.
Embodiment
Spherical Rhodopseudomonas can carry out non-oxygen-production photosynthesis in the illumination anoxic environment is arranged, compare with higher plant, and it also contains and similarly catches photopigment, but has only a photosystem, i.e. PSI, and original hydrogen donor is not a water, but H
2S (or some organic matters).Spherical Rhodopseudomonas has been finished again and has been produced very important chemical process in hydrogen, fixed nitrogen, three natural world material circulations of decomposing organic matter in the assimilation process of self.These features of spherical Rhodopseudomonas have remedied corn sow thistle and absorbed the phenomenon that overground part photosynthesis that oligotrophy causes weakens because of root system in soil lead pollution adverse circumstances, improve leaf chlorophyll and carotenoid content greatly, strengthen photosynthetic rate, and promote the growth of corn sow thistle root system conversely, thereby reach the raising resistance, the purpose that increases production and improves productivity.Therefore spherical Rhodopseudomonas anaerobism illumination cultivation does not need general microbial inoculum to produce required large-scale instrument and equipments such as fermentation tank, and production cost is very low, production process safety is used the back environmental friendliness, and is efficient again, simple to operate, be the first-selected way that solves lead-contaminated soil plantation corn sow thistle problem.
The screening of high-effective microorganism and fungicide preparation: at first screening is suitable for the micropopulation of corn sow thistle foliage-spray, increase yield significantly, screening one by one from this micropopulation again obtains a strain promotes that significantly corn sow thistle is grown in lead-contaminated soil bacterial strain---spherical Rhodopseudomonas (Rhodopseudomonasspheroides) SH II-7.The medium of fungicide preparation is: NH
4Cl 1.0g, CaCl
20.02g, MgSO
47H
2O 0.2g, NaHCO
33.0g, K
2HPO
40.4g, NaCl 1.0g, peptone 1.0g, water 1000mL, PH neutrality was sterilized 30 minutes for 121 ℃.Anaerobism illumination cultivation 4-5 days, acquisition concentration is 1-2 * 10
8The microbial inoculum of individual viable bacteria/ml.Introduce specific embodiments of the invention below in detail.
Embodiment 1
Before the corn sow thistle sowing, get 10 times of seed soaking of microbial inoculum dilution, 500 times of microbial inoculum dilutions after this, from two leaf phases, per 2 all foliage-sprays 1 time spray 4 times continuously, and after this, per 3 all foliage-sprays 1 time are until the end of gathering.
Embodiment 2
In higher concentration lead-contaminated soil (as 1200mgPb/kg soil), shown just that with plant poor growth, blade hour rise, get 200 times of microbial inoculum dilutions, foliage-spray is 1 time weekly, spray 2 times continuously and stop, when extremely once more poor growth taking place, get 500 times of microbial inoculum dilutions, foliage-spray is 1 time weekly, sprays 2 times continuously and stops.
Management such as other liquid manure is normal.
Claims (3)
1. the foliage-spray microorganism is improved the method for the anti-lead of corn sow thistle, it is characterized in that this method microorganism is spherical Rhodopseudomonas (Rhodopseudomonas spheroides) SH II-7, anaerobism illumination cultivation 4-5 days, and acquisition concentration is 1-2 * 10
8The microbial inoculum of individual viable bacteria/ml before the corn sow thistle sowing, is got 10 times of seed soaking of microbial inoculum dilution, and 200-500 times of microbial inoculum dilution after this is at corn sow thistle growth period foliage-spray.
2. foliage-spray microorganism according to claim 1 is improved the method for the anti-lead of corn sow thistle, it is characterized in that the medium of fungicide preparation is: NH
4Cl 1.0g, CaCl
20.02g, MgSO
47H
2O0.2g, NaHCO
33.0g, K
2HPO
40.4g, NaCl 1.0g, peptone 1.0g, water 1000ml, PH neutrality was sterilized 30 minutes for 121 ℃, anaerobism illumination cultivation 4-5 days, acquisition concentration is 1-2 * 10
8The microbial inoculum of individual viable bacteria/ml.
3. foliage-spray microorganism according to claim 1 is improved the method for the anti-lead of corn sow thistle, it is characterized in that the microbial inoculum application process is: before the corn sow thistle sowing, get 10 times of seed soaking of microbial inoculum dilution, after this microbial inoculum dilution 200-500 doubly, begin from two leaf phases, every 2-3 week foliage-spray 1 time is until the end of gathering; Or plant rises when just having shown poor growth, and foliage-spray is 1 time weekly, sprays 2 times continuously and stops.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102171139A CN101940113A (en) | 2010-07-05 | 2010-07-05 | Method for improving lead resistance of endive by spraying microorganism on blade surface |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102171139A CN101940113A (en) | 2010-07-05 | 2010-07-05 | Method for improving lead resistance of endive by spraying microorganism on blade surface |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101940113A true CN101940113A (en) | 2011-01-12 |
Family
ID=43432477
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010102171139A Pending CN101940113A (en) | 2010-07-05 | 2010-07-05 | Method for improving lead resistance of endive by spraying microorganism on blade surface |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742439A (en) * | 2012-06-29 | 2012-10-24 | 沈阳化工大学 | Method for improving lead resistance of endive by applying photosynthetic bacteria |
| CN103004420A (en) * | 2012-12-14 | 2013-04-03 | 沈阳化工大学 | Method for improving tolerance of soybean to petroleum and lead contaminated soil by applying photosynthetic bacteria |
-
2010
- 2010-07-05 CN CN2010102171139A patent/CN101940113A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742439A (en) * | 2012-06-29 | 2012-10-24 | 沈阳化工大学 | Method for improving lead resistance of endive by applying photosynthetic bacteria |
| CN102742439B (en) * | 2012-06-29 | 2014-07-02 | 沈阳化工大学 | Method for improving lead resistance of endive by applying photosynthetic bacteria |
| CN103004420A (en) * | 2012-12-14 | 2013-04-03 | 沈阳化工大学 | Method for improving tolerance of soybean to petroleum and lead contaminated soil by applying photosynthetic bacteria |
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Application publication date: 20110112 |