CN101921821A - Method for extracting high-quality collagen at low temperature - Google Patents
Method for extracting high-quality collagen at low temperature Download PDFInfo
- Publication number
- CN101921821A CN101921821A CN 201010248083 CN201010248083A CN101921821A CN 101921821 A CN101921821 A CN 101921821A CN 201010248083 CN201010248083 CN 201010248083 CN 201010248083 A CN201010248083 A CN 201010248083A CN 101921821 A CN101921821 A CN 101921821A
- Authority
- CN
- China
- Prior art keywords
- collagen
- low temperature
- extracting high
- enzymolysis
- quality
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a method for extracting high-quality collagen at the low temperature, comprising the following steps of: taking fresh or dry fish scales and the like, and carrying out enzymolysis treatment with an enzymolysis liquid, wherein the temperature of the enzymolysis reaction in between 4 DEG C and 49 DEG C; and filtering the enzymolysis liquid after the enzymolysis treatment to obtain a collagen liquid, and drying to obtain collagen powder. The invention has the advantages of security, convenience and environment protection, and enables the activity of the collagen to be reserved to the utmost extent.
Description
Technical field:
The present invention relates to a kind of method of extracting high-quality collagen.
Background technology:
Collagen protein is one of most important protein in the human body, now has been widely used in fields such as medicine, biomaterial, food, daily-use chemical industry.The main means of the collagen protein of countries in the world extraction at present are to take strong acid and strong base, pyroprocessing and enzymolysis etc., and adopt mad cow disease, foot and mouth disease cause of disease hidden danger raw materials such as pig, ox bone and tendon in a large number.
Because acid-alkali treatment contaminate environment, enzymolysis are carried out under 60 degree left and right sides hot conditionss usually, present extracting method is the serious environment pollution and a large amount of energy of consumption not only, and the extraction under conditions such as soda acid high temperature, have destroyed the natural bioactive of collagen protein.
Summary of the invention:
The object of the present invention is to provide a kind of extract at low temperature method of extracting the high-quality collagen of convenience, environmental protection, reservation collagen protein natural radioactivity.
Technical solution of the present invention is:
A kind of method of extracting high-quality collagen at low temperature is characterized in that: comprise the following steps:
(1) get fish scale or other animal materials that contains collagen protein of cleaning, carry out enzymolysis processing with enzymolysis solution, the temperature of enzyme digestion reaction is 4～49 ℃;
(2) after the enzymolysis processing enzymolysis solution is filtered, obtain collagen protein liquid, drying obtains the collagen protein powder.
Described fish scale is fresh or the exsiccant fish scale.
The time of enzyme digestion reaction is 1～19 hour, and the pH value of enzymolysis solution is 3～9.
Enzyme is a kind of or several mixtures in metalloprotease, thiol proteinase, zinc metalloprotein enzyme, serine carboxypeptidase, kethepsin, serine protease, collagenase, endopeptidase, ficin, the pepsin.
The amount of enzymolysis solution is 1～19 times (being that solid-liquid ratio is 1: 1～19) of fish scale weight.
The weight of enzyme is 0.01～10: 100 with the ratio of the weight of fish scale.
The invention has the advantages that:
1. under low temperature (normal temperature) condition, utilization selectivity multidigit point enzyme incision technology extracts the collagen protein of biologically active.Promptly, regulate and control final product molecular weight and functional fragment by selective enzyme restriction macromolecular collagen protein different loci.Owing under cold condition, extract, make the collagen protein biological activity maximize the retention.
2. abandon high temperature and strong acid and strong base and handle saving equipment, the energy, cost and labor force, and environmental protection.
3. do not add any chemical substance, do not carry out the soda acid desalting treatment, keep natural trace element and biologically active substance.
4. China is maximum in the world fishery products country of origin, and the present invention adopts raw material to be mainly derived from the processing of aquatic products residuum, belongs to recycling economy and green economy, and abandons mad cow disease, foot and mouth disease cause of disease hidden danger raw materials such as pig, ox bone and tendon fully.
Embodiment:
A kind of method of extracting high-quality collagen at low temperature comprises the following steps:
(1) choose fresh or the exsiccant fish scale, carry out enzymolysis processing with enzymolysis solution, the temperature of enzyme digestion reaction is 4～49 ℃ (4 ℃, 20 ℃, 40 ℃, 49 ℃ of examples); The time of enzyme digestion reaction is 1～19 hour (example 1 hour, 9 hours, 19 hours), and the pH value of enzymolysis solution is 3～9 (examples 3,6,9), if not in this scope, can use HCl or NaOH to regulate.The amount of enzymolysis solution is 1～19 times (1 times, 5 times, 9 times of example) of fish scale weight; The weight of enzyme is 0.01～10: 100 (examples 0.01: 100,5: 100,10: 100) with the ratio of the weight of fish scale; Enzyme is a kind of or several mixtures in the enzymes such as metalloprotease, thiol proteinase, zinc metalloprotein enzyme, serine carboxypeptidase, kethepsin, serine protease, collagenase, endopeptidase, ficin, pepsin.
(2) after the enzymolysis processing enzymolysis solution filtration is obtained collagen protein liquid, filtering accuracy scope: 0.15 μ m-150 μ m, the filtrate drying obtains the collagen protein powder.
Raw material involved in the present invention can also be the no mad cow disease beyond pig, ox bone and the tendon, the animal material of foot and mouth disease hidden danger, as fish-skin and donkey hide etc.
Claims (6)
1. the method for an extracting high-quality collagen at low temperature is characterized in that: comprise the following steps:
(1) get fish scale or other animal materials that contains collagen protein of cleaning, carry out enzymolysis processing with enzymolysis solution, the temperature of enzyme digestion reaction is 4～49 ℃;
(2) after the enzymolysis processing enzymolysis solution is filtered, obtain collagen protein liquid, drying obtains the collagen protein powder.
2. the method for extracting high-quality collagen at low temperature according to claim 1, it is characterized in that: described fish scale is fresh or the exsiccant fish scale.
3. the method for extracting high-quality collagen at low temperature according to claim 1, it is characterized in that: the time of enzyme digestion reaction is 1～19 hour, the pH value of enzymolysis solution is 3～9.
4. the method for extracting high-quality collagen at low temperature according to claim 1, it is characterized in that: enzyme is a kind of or several mixtures in metalloprotease, thiol proteinase, zinc metalloprotein enzyme, serine carboxypeptidase, kethepsin, serine protease, collagenase, endopeptidase, ficin, the pepsin.
5. according to the method for claim 1,2,3 or 4 described extracting high-quality collagen at low temperature, it is characterized in that: the amount of enzymolysis solution is 1～19 times of raw material weight.
6. according to the method for claim 1,2,3 or 4 described extracting high-quality collagen at low temperature, it is characterized in that: the weight of enzyme is 0.01～10: 100 with the ratio of the weight of raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010248083 CN101921821A (en) | 2010-08-06 | 2010-08-06 | Method for extracting high-quality collagen at low temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010248083 CN101921821A (en) | 2010-08-06 | 2010-08-06 | Method for extracting high-quality collagen at low temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101921821A true CN101921821A (en) | 2010-12-22 |
Family
ID=43337036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010248083 Pending CN101921821A (en) | 2010-08-06 | 2010-08-06 | Method for extracting high-quality collagen at low temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101921821A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559820A (en) * | 2011-12-22 | 2012-07-11 | 上海东锦食品集团有限公司 | Preparation method and applications of nano collagen with oxidation resistance capacity |
| CN102552987A (en) * | 2012-01-12 | 2012-07-11 | 福原理加 | Biological material in liquid at 4 DEG C and solid at 37 DEG C and preparation method and application |
| CN102586373A (en) * | 2012-01-16 | 2012-07-18 | 郁小兵 | Method for extracting high-quality collagen at low temperature |
| CN103146789A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Preparation method of collagen |
| CN103315126A (en) * | 2013-06-09 | 2013-09-25 | 江南大学 | Preparation method for low-molecular-weight fish hydrolyzed protein powder |
| WO2020207370A1 (en) * | 2019-04-08 | 2020-10-15 | Novozymes A/S | Method for extracting gelatin |
| CN112056550A (en) * | 2020-07-27 | 2020-12-11 | 光亚生物科技(广州)有限公司 | A tonic small molecular deer gelatin paste and its preparation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814782A (en) * | 2005-11-28 | 2006-08-09 | 海南莱顿生物技术有限公司 | Method for extracting small molecule collagen protein from fish scale utilizing enzyme engineering technique |
-
2010
- 2010-08-06 CN CN 201010248083 patent/CN101921821A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814782A (en) * | 2005-11-28 | 2006-08-09 | 海南莱顿生物技术有限公司 | Method for extracting small molecule collagen protein from fish scale utilizing enzyme engineering technique |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559820A (en) * | 2011-12-22 | 2012-07-11 | 上海东锦食品集团有限公司 | Preparation method and applications of nano collagen with oxidation resistance capacity |
| CN102552987A (en) * | 2012-01-12 | 2012-07-11 | 福原理加 | Biological material in liquid at 4 DEG C and solid at 37 DEG C and preparation method and application |
| CN102552987B (en) * | 2012-01-12 | 2016-01-06 | 郁小兵 | 4 DEG C are in a liquid state 37 DEG C in solid-state biomaterial and preparation method with purposes |
| CN102586373A (en) * | 2012-01-16 | 2012-07-18 | 郁小兵 | Method for extracting high-quality collagen at low temperature |
| CN103146789A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Preparation method of collagen |
| CN103146789B (en) * | 2013-02-28 | 2014-11-26 | 珠海博康药业有限公司 | Preparation method of collagen |
| CN103315126A (en) * | 2013-06-09 | 2013-09-25 | 江南大学 | Preparation method for low-molecular-weight fish hydrolyzed protein powder |
| CN103315126B (en) * | 2013-06-09 | 2014-08-13 | 江南大学 | Preparation method for low-molecular-weight fish hydrolyzed protein powder |
| WO2020207370A1 (en) * | 2019-04-08 | 2020-10-15 | Novozymes A/S | Method for extracting gelatin |
| CN113544226A (en) * | 2019-04-08 | 2021-10-22 | 诺维信公司 | Method for extracting gelatin |
| CN112056550A (en) * | 2020-07-27 | 2020-12-11 | 光亚生物科技(广州)有限公司 | A tonic small molecular deer gelatin paste and its preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101921821A (en) | Method for extracting high-quality collagen at low temperature | |
| Je et al. | Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis | |
| CN104003766B (en) | The waste water of livestock and poultry harmless treatment and meat meal tankage is utilized to produce the method for amino acid fertilizer | |
| CN102669663B (en) | Method for preparing active calcium powder by using teleostean chine | |
| Adetunji et al. | Efficacy of crude and immobilizedenzymes from Bacillus licheniformis for production of biodegraded feather meal and their assessment on chickens | |
| CN101407834A (en) | Method for preparing collagen peptide by using cod skin | |
| CN102146117B (en) | Method for preparing lysosomal collagen | |
| CN100369929C (en) | Jellyfish collagen and method for preparing the same | |
| CN101538601A (en) | Method for producing bovine-bone peptone by enzyme technology | |
| CN103755396B (en) | A kind of preparation method of the Lumbricus complex liquid promoting wheat increase yield | |
| CN109354624A (en) | The method that cattle hide gelatin is prepared using enzymatic treatment | |
| CN104212862A (en) | Wood frog protein and bone calcium extraction method | |
| CN109247011B (en) | Method for producing amnion-derived material and method for producing health food | |
| CN102154425A (en) | Production method of non-denatured II-type collagen | |
| EP2210678A1 (en) | Biotechnological method for the processing of meat by-products and resulting product | |
| TWI362393B (en) | A new process for the preparation of collagen peptide from fish scale | |
| Raju et al. | Enzymatic hydrolysis of tannery fleshings using chicken intestine proteases | |
| CN102586373A (en) | Method for extracting high-quality collagen at low temperature | |
| CN106495517B (en) | A kind of utilization method of slaughtering waste | |
| WO2011108810A2 (en) | Amino acid liquid fertilizer using marine waste, and preparation method thereof | |
| CN103919808B (en) | A kind of preparation method of liver extract | |
| CN103667225A (en) | Method for preparing elastase | |
| Kim et al. | Marine‐derived peptides: Development and health prospects | |
| CN107325158A (en) | Antibacterial peptide CNAP I, antibacterial peptide CNAP II and its preparation method and application | |
| US20190263891A1 (en) | Process for isolating bioactive biomolecules from animal by-products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| ASS | Succession or assignment of patent right |
Owner name: JIANGSU ALPHAY BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: YU XIAOBING Effective date: 20101227 |
|
| C10 | Entry into substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yu Xiaobing Inventor after: Zheng Huihua Inventor after: Xu Chunhua Inventor after: Zhang Chenju Inventor after: Pan Rong Inventor after: Chen Hui Inventor before: Yu Xiaobing |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: YU XIAOBING TO: YU XIAOBING ZHENG HUIHUA XU CHUNHUA ZHANG CHENJU PAN RONG CHEN HUI Free format text: CORRECT: ADDRESS; FROM: 100000 14-2-403, BEITOUTIAO, TUANJIEHU, CHAOYANG DISTRICT, BEIJING TO: 226009 NO.68-A, ZHONGYANG ROAD, ECONOMIC DEVELOPMENT ZONE, NANTONG CITY, JIANGSU PROVINCE |
|
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20101227 Address after: 226009 68-A, Central Road, Nantong Economic Development Zone, Jiangsu, China Applicant after: Jiangsu Alphay Biotechnology Co., Ltd. Address before: 100000 Beijing Chaoyang District solidarity Hubei headlines 14-2-403 Applicant before: Yu Xiaobing |
|
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20101222 |