CN101914164A - Application of polysaccharide from mulberry leaves in preventing and treating diabetes - Google Patents
Application of polysaccharide from mulberry leaves in preventing and treating diabetes Download PDFInfo
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Abstract
The invention relates to application of polysaccharide extracted from mulberry leaves in preventing and treating diabetes, belonging to the natural medicine application field. The application method comprises the following steps: crushing 10kg of the dry mulberry leaves, and adding 180L of distilled water to the crushed mulberry leaves; heating and then carrying out reflux extraction on the obtained mixture at the temperature of 70 DEG C for 90min; filtering the obtained extracting solution twice with three-layer gauze and then filtering with a ceramic membrane; decompressing and concentrating the obtained filtrate into 10L, and then deproteinizing the obtained concentrated solution with 10% of trichloroacetic acid; and placing the concentrated solution on an SEPHADEX G-10 column for separating and collecting components containing the polysaccharide from the mulberry leaves, concentrating the solution into 1L, and then placing the concentrated solution on a silicagel column for separating and collecting the components containing the polysaccharide from the mulberry leaves again to obtain the polysaccharide from the mulberry leaves, wherein, content of the polysaccharide from the mulberry leaves is 95.5%.
Description
Affiliated technical field:
The present invention relates to a kind of application of polysaccharides of Folium Mori in prevention and treatment diabetes of from mulberry leaf, extracting, belong to the technical field that natural drug is used.
Background technology
So far, global diabetic subject has reached 1.3 hundred million, and number is also increasing severely.Diabetes have become No. three killer after cardiovascular, tumour, are one of the world's three big bus health difficult problems.According to Ministry of Health's publish data, 2009 are only the city diabetic subject surpasses 4,100 ten thousand, and newly-increased every year on average patient about 1,500,000.Diabetes are the common diseases that caused by the h and E factor interaction, and clinical is item key with the hyperglycemia, and common sympton has many drinks, diuresis, eats more and becomes thin etc.Diabetes can cause the infringement of health multisystem, cause that absolute or relative hyposecretion of Regular Insulin and target tissue cell reduce insulin sensitivity, cause a series of metabolism disorder syndromes such as protein, fat, power and water Xie Zhi, wherein a hyperglycemia is an item key.Also there is not the specific medicine can give effective radical cure at present.From existing screening bioactive compounds, study the herbal medicine efficacy composition of known curative effect, have very great help to promoting the internationalization of tcm tool.
In the past, treatment of diabetes was mainly contained four kinds of methods: dietetic treatment, pharmacological agent, exercise therapy.Abroad, some famous physicians advocate that the diabetic subject should do suitable physical exertion, and physical exertion, dietary control, insulin injection are classified as the three magic weapons of treatment diabetes.In recent years, utilize the Chinese medicine diabetes to become the hot subject of research.
Polysaccharides of Folium Mori is a kind of acid heteroglycan, mainly is made up of uronic acid and various neutral sugar.Mulberry leaf different name Herba adianti myriosori is the Moraceae fallen leaves property leaf of grass mulberry tree for many years.Modern pharmacology and clinical study show, that the polysaccharide of one of mulberry leaf activeconstituents has is hypoglycemic, hypotensive, immunomodulatory, multiple physiologically active such as antibiotic and antiviral.Especially Japan is more to the effective constituent research in the mulberry leaf both at home and abroad at present, finds that polysaccharide has hypoglycemic effect, and polysaccharide structures is analyzed.Therefore be subjected to the attention on biomedical boundary.
Polysaccharide be a class by the biological response modifier of active research, the active polysaccharide that contains in the mulberry leaf has raise immunity and anti-infectious effect, treats the effect of hypercholesterolemia in addition, and can strengthen blood vessel and prevention of arterial sclerosis, thrombosis, scheming infarction etc.The polysaccharides of Folium Mori nontoxicity has tangible health-care effect, especially has the immunocompetence function and comes into one's own more, and the polysaccharides of Folium Mori protective foods will become important a member of novel healthy food.Therefore the mulberry leaf resource of developing us just has crucial effect.
China's mulberry tree kind, kind and mulberry fruit output all occupy first place, the world, and except that the Qinghai-Tibet Platean, all there is cultivation all parts of the country.Most of at present area is with the feeding cultivation of Sang Zuowei, and is annual except raising silkworm, and a lot of positions of mulberry tree all do not obtain utilizing.Although many researchs have reported that mulberry has multiple pharmacological effect, the research of anti-allergic effects also rarely has report.Document up to now focuses on that all the 1-S-GI that extracts in the mulberry leaf studies in the treatment diabetes, its mechanism of action in the treatment diabetes is fully aware of.We find the better effects if of the high 1-S-GI of the low 1-S-GI ratio content of content to the treatment diabetes in research process, consult document simultaneously and find polysaccharides of Folium Mori in not research aspect prevention and the treatment diabetes.And polysaccharides of Folium Mori content in mulberry leaf is higher, draws materials conveniently, and is widely distributed, low price.Thereby, if polysaccharides of Folium Mori has certain effect at tool aspect prevention and the treatment diabetes, will mean a great to the Sang Jinhang deep processing.
Summary of the invention
The polysaccharides of Folium Mori that the present invention mentioned is to remove albumen from drying mulberry leaf later through using the extraction of distillation hydro-thermal, filtration, concentrating under reduced pressure, trichoroacetic acid(TCA), adsorbs, elutriant is concentrated with post then, and concentrating under reduced pressure makes the pure product of polysaccharides of Folium Mori.Its polysaccharide content is more than 95%.
Technique effect:
1, used raw material, equipment is common common raw material, equipment in the inventive method, has avoided in the commercial process having reduced production cost widely for the dependence of expensive raw materials, instrument.
2, mulberry tree plantation easily, cuttage, seed broadcast all can, output height, price are low, convenient sources, follow-up resource is secure.Compare with traditional extraction technology, this technology can shorten extraction time greatly, cut down the consumption of energy, reduces the stripping of impurity component.
3, the inventive method is simple to operate, does not need precision instrument or automatic equipment, can produce in the resourceful township and village enterprises of mulberry leaf, and this greatly reduces the production cost of polysaccharides of Folium Mori, has simplified production process.
4, polysaccharides of Folium Mori of the present invention has many-sided opsonization, and side effect is little, compares with chemical synthetic drug, does not have tangible dependency.
Embodiment
Below, the present invention will be further detailed with embodiment, but it is not limited to any or the similar example of these embodiment.
Embodiment 1: the preparation method of polysaccharides of Folium Mori
Pulverize dry mulberry leaf 10kg, add 180L distilled water, 70 ℃ of heating and refluxing extraction 90min, three layers of filtered through gauze twice are used ceramic membrane filter again, are evaporated to 10L, concentrated solution removes albumen with 10% trichoroacetic acid(TCA) then, last SEPHADEX G-10 post separated and collected contains the polysaccharides of Folium Mori component, is concentrated into 1L and goes up silicagel column separated and collected polysaccharides of Folium Mori composition again and be concentrated into driedly, obtains polysaccharides of Folium Mori.Measure through anthrone-vitriol oil method, wherein polysaccharides of Folium Mori content is 95.5%.
Embodiment 2: the mouse of polysaccharides of Folium Mori is fed experiment
1, diabetic mice model
(1) adult kunming mice (20-25g) is cultured under (25 ± 1 ℃) ventilation condition, illumination 12h, and dark 12h, free choice feeding, standard are fed.
(2) diabetic mice is induced: according to the requirement of 45mg/kg, by abdominal injection U-9889 (being dissolved in 0.1M citrate buffer solution PH4.5).Continuous 7 days, mouse tail blood sampling on an empty stomach, the mouse of selecting fasting plasma glucose 200mg/dl is as experimental subjects.2, experimental design:
2, experimental mouse is divided into 6 groups, 6 every group: test the line space abdomen blood sugar detection that advances
The 1st group: normal non-diabetic mouse, drink distilled water;
The 2nd group: diabetic mice, distilled water;
The 3-5 group: diabetic mice is given Folium Mori extract 0.25,0.5,1.0g/kg/day respectively;
The 6th group: diabetic mice injection 4U/kg/day Regular Insulin continued for 6 weeks.
All experimental mouse use the blood sugar detection instrument to measure blood sugar before experiment and the 6th all laggard line space abdomen blood sugar detections.During the fasting, stop to all foods of mouse except water.After initial period (before inducing diabetes) and 6 all treatments phases, caudal vein is got hematometry mda, lipid peroxide and glycolated hemoglobin.
3, use the production of thiobarbituric acid reactive method to detect MDA: blood plasma and thiobarbituric acid reaction generate colored production of thiobarbituric acid reactive (describing according to Draper), the 532nm wavelength is measured down, and contents of mda is used the tetraethoxypropane typical curve.
4, lipid peroxide is measured and is adopted the chemical examination of ferrous ion xylenol orange: blood plasma lipide peroxide oxidation Fe
2+To Fe
3+Bonding xylenol orange (describing) according to Nourooz-Zadeh.Reactant is spectrophotometric determination under the 560nm wavelength.Lipid peroxide contents is used the hydrogen peroxide standard curve determination.Measure in the body Folium Mori extract to the effect of saccharification level by the method for chemical examination glycolated hemoglobin.The total antithrombotics of blood is used for measuring protoheme and glycolated hemoglobin concentration.The protoheme that colorimetric method for determining is total uses the turbid rate method of immune transmittance (COBAS INTEGRA 700 test kits) to measure glycolated hemoglobin.The glycolated hemoglobin amount is represented with the % content of protoheme.
5, experimentation on animals result
(1) the mulberry leaf extracting solution is to the influence of tissue of experimental diabetic mice blood sugar
With diabetic model group relatively, mulberry leaf extracting solution group whole blood blood sugar obviously descends (P＜0.05), more experimental mouse is drunk, is eaten more, the diuresis symptom is obviously improved.But blood sugar and control group are more higher, do not drop to normal level, and play a part hypoglycemic obvious more along with the increase of Folium Mori extract concentration within the specific limits; But its effect does not have the obvious of Regular Insulin.Blood sugar no significant difference (P＞0.05) before and after the vacation of diabetic model group experimental mouse is raised.The results are shown in Table 1:
Table 1 mulberry leaf extracting solution group is to the influence of artificial diabetes experimental mouse blood sugar
Table 1 The effects of extract of mulberry leaves on blood sugar level in diabetic rats (Jian x ± s, mmol/L)
(2) the mulberry leaf extracting solution is to the influence of tissue of experimental diabetic mice activity of plasma SOD
Diabetic groups experimental mouse SOD activity after the modeling obviously reduces than the SOD of control group experimental mouse is active, gives active obviously raise (P＜0.05) of whole blood SOD behind the mulberry leaf extracting solution.The amount difference of giving of its Folium Mori extract also has this different effect.The results are shown in Table 2:
Table 2 mulberry leaf extracting solution group is to the influence of artificial diabetes experimental mouse activity of plasma SOD
Table2 The effects of extract of mulberry leaves on SOD in plasma of diabetic rats (Jian x ± s, U/mL)
Diabetic mice model SOD level is lower than normal experimental mouse SOD level as can be seen from Table 2, give the mulberry leaf extracting solution after, the activity of plasma SOD level of experimental mouse obviously raise (P＜0.05).Its active size that raises within the specific limits along with Folium Mori extract to measuring into the forward dependency, the SOD activity of Folium Mori extract group 3 has reached normal level.The injection of insulin group then makes its SOD activation recovering to normal level.
(3) the mulberry leaf extracting solution is to the influence of tissue of experimental diabetic mice whole blood LPO content
Diabetic groups experimental mouse LPO content after the modeling obviously raises than the LPO content of control group experimental mouse, gives behind the mulberry leaf extracting solution whole blood LPO content obviously descend (P＜0.05).The amount difference of giving of its Folium Mori extract also has this different effect.The results are shown in Table 3:
Table 3 mulberry leaf extracting solution group is to the influence of artificial diabetes experimental mouse whole blood LPO content
Table 3 The effects of extract of mulberry leaves on LPO in diabetic rats (Jian x ± s, mmol/L)
As shown in Table 3, Folium Mori extract has certain effect to the reduction of LPO in the blood glucose in diabetic mice, and along with the rising of concentration, its effect is also obvious more, and the LPO content of Folium Mori extract group 3 also approaches normal level.Glycosuria pathology group is no change then, and the LPO content of injection of insulin group mouse is then near normal level.
Claims (3)
1. the novel preparation method of a polysaccharides of Folium Mori, and it is used for preventing and treating the application of diabetes, it comprises the following steps:
Pulverize dry mulberry leaf 10kg, add 180L distilled water, 70 ℃ of heating and refluxing extraction 90min, three layers of filtered through gauze twice are used ceramic membrane filter again, are evaporated to 10L, concentrated solution removes albumen with 10% trichoroacetic acid(TCA) then, last SEPHADEX G-10 post separated and collected contains the polysaccharides of Folium Mori component, is concentrated into 1L and goes up silicagel column separated and collected polysaccharides of Folium Mori composition again and be concentrated into driedly, obtains polysaccharides of Folium Mori.
2. the novel preparation method of a kind of polysaccharides of Folium Mori according to claim 1 is characterized in that: the trichoroacetic acid(TCA) isolating protein of available different concns.
3. the novel preparation method of a kind of polysaccharides of Folium Mori according to claim 1 is characterized in that: directly go up silicagel column and separate the polysaccharides of Folium Mori component.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103127227A (en) * | 2011-11-28 | 2013-06-05 | 山东农业大学 | Preparation method for mulberry leaf polysaccharide hypoglycemic active component |
| CN112220825A (en) * | 2019-07-14 | 2021-01-15 | 北京恒润泰生医药科技有限公司 | Mulberry leaf aqueous extract powder, preparation, immune enhancement effect and application thereof |
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| CN101712726A (en) * | 2009-10-30 | 2010-05-26 | 浙江工业大学 | Method for preparing high-purity mulberry leaf polysaccharide |
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| CN101914164B (en) | 2013-03-27 |
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