CN101898829A - Method for starting salt tolerant anammox reactor - Google Patents

Abstract

The invention discloses a method for starting salt tolerant anammox reactor applied to biological nitrogen removal of high salinity waste water (such as mariculture waste water and aquatic product processing waste water). Inoculated sludge is placed into an anammox reactor, the inoculated sludge comprises 1.65-69.12g/L of MLSS and 0.687-21.33g/L of MLVSS, operational conditions include that anaerobic environment is provided, temperature is 32.5-33.5 DEG C, HRT is two days, the concentrations of NH4+-N and NO2--N in inlet water are all 60-70mg/L and continuous culture is carried out until the concentrates of NH4+-N and NO2--N in effluent are all lower than 10mg/L; the concentrates of NH4+-N and NO2--N in inlet water are improved to be 70-160mg/L, HRT is 1-1.5 days, continuous culture is carried out for 20-50 days, the salinity of inlet water is regulated to be 5g/kg, 10g/kg, 15g/kg, 20g/kg, 25g/kg and 30g/kg respectively by virtue of sea water (the salinity of full sea water is 32g/kg), and continuous culture is respectively carried out on inlet water at each salinity until the concentrations of the NH4+-N and NO2--N in effluent are all lower than 20mg/L.

Description

The startup method of salt tolerant anammox reactor

Technical field:

The present invention relates to a kind of startup method of anaerobic ammonia oxidation reactor, especially a kind of startup method that can be applicable to the salt tolerant anammox reactor of high salinity waste water (as marine culture wastewater, aquatic products processing wastewater etc.) biological denitrificaion.

Background technology:

Anammox bio-denitrifying sewage technology, economy efficient because of it, characteristics that secondary pollution is few are subjected to the extensive concern of domestic and international sewage treatment area in recent years.At present, applied anaerobic ammonia oxidation reactor is generally cylindrical, built-in nonwoven strip filler, and the side is provided with thief hole, and effective volume is 5L～6L.Reactor is by the insulation of water bath with thermostatic control chuck, and water inlet is continuously pumped into by BT-200 type constant flow pump.The inflated with nitrogen deoxygenation makes whole device be in anaerobic environment in the reaction process, need use gobo encapsulation reaction device simultaneously, to prevent the restraining effect of light to anaerobic ammonia oxidizing bacteria.In practical engineering application, the startup of reactor (biomembranous cultivation) is the committed step of reactor operation, promptly need bacterial classification is put in the anaerobic ammonia oxidation reactor, inject nutrient solution continuously from the anaerobic ammonia oxidation reactor bottom, organic and the inorganic particle mud that is had with reactor itself is as the microorganism growth carrier, microbiological anaerobic is cultured to could handle waste water after forming microbial film under specific temperature conditions.

Function bacterial classification-the anaerobic ammonia oxidizing bacteria that is applied at present in the anaerobic ammonia oxidation process derives from fresh water environment mostly, comprises sludge sewage, anaerobically fermenting granule sludge, bed mud in river etc., is difficult to adapt to the higher sewage disposal of salinity.Although, be mostly to investigate with the simulated wastewater that adds NaCl or KCl to the existing report of the salt tolerance of this bacterium.Yet seawater is a kind of natural haline water, and its salinity is generally 30～35 ‰, not only contains K ⁺, Na ⁺, Cl ^-Ion also contains Ca ²⁺, Mg ²⁺, Sr ²⁺, Br ^-, CO ₃ ^2-(HCO ₃ ^-), F ^-, SO ₄ ^2-Etc. multiple ion and HBO greater than 0.05mmol/kg ₃Molecule, the component complexity, the existing for this reason investigation about salt tolerance is difficult to drop into actual production.

Summary of the invention:

The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of startup method that can be applicable to the salt tolerant anammox reactor of high salinity waste water (as marine culture wastewater, aquatic products processing wastewater etc.) biological denitrificaion to be provided.

Technical solution of the present invention is: a kind of startup method of salt tolerant anammox reactor is characterized in that carrying out as follows:

A. seed sludge is inserted in the anaerobic ammonia oxidation reactor, MLSS is that 1.65～69.12g/L, MLVSS are 0.687～21.33g/L in the seed sludge, operational conditions: anaerobism, and 33 ± 0.5 ℃ of temperature, HRT is 2 days, NH in the water inlet ₄ ⁺-N and NO ₂ ^--N concentration is 60～70mg/L, and cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 10mg/L;

B. NH in will intaking ₄ ⁺-N and NO ₂ ^--N concentration all rises to 70～160mg/L, and HRT is 1～1.5 day, cultured continuously 20～50 days, regulate the water inlet salinity with seawater again and be followed successively by 5g/kg, 10g/kg, 15g/kg, 20g/kg, 25g/kg, 30g/kg, full seawater and respectively to each salinity cultured continuously to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L.

The present invention progressively improves the purpose that the water inlet salinity reaches the seawater acclimation anaerobic ammonia oxidizing bacteria with seawater, even anaerobic ammonia oxidizing bacteria progressively adapts to the seawater that also contains other salinity except that NaCl, solved the deficiency that prior art only exists as quantitative salinity with NaCl, can be efficient with having, economical, the Anammox bio-denitrifying sewage process application of characteristics such as secondary pollution is few is handled in high salinity waste water, as the marine culture wastewater biological denitrificaion is handled, aquatic products processing wastewater biological denitrificaion processing etc. have enlarged the range of application of Anammox wastewater biological denitrificaion technology.

Embodiment:

Embodiment 1:

A. adopt existing anaerobic ammonia oxidation reactor, effective volume is 5L, and seed sludge is taken from development area, Dalian sewage work anoxic pond, and the character of seed sludge: MLSS is 69.12g/L, and MLVSS is 21.33g/L.The reactor start-up condition: anaerobism, 33 ± 0.5 ℃ of temperature, HRT is 2 days, to change NH ₄Cl and NaNO ₃Dosage is regulated water inlet NH ₄ ⁺-N and NO ₂ ^--N concentration is 60～70mg/L.Monitor water outlet NH simultaneously ₄ ⁺-N and NO ₂ ^--N concentration was cultivated through 174 days, adhered to mycoderm on the reactor carrier and became sorrel by black, water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 10mg/L;

B. improve water inlet NH ₄ ⁺-N and NO ₂ ^--N concentration is 70～100mg/L, and regulating HRT is 1.5 days, and cultured continuously 20 days with the earlier quantitative sea water salinity of salometer, is added seawater again in water inlet, and recently regulating the water inlet salinity with the seawater and freshwater volume is 5g/kg, and cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L; Improve again the seawater and freshwater volume recently regulate the water inlet salinity be 10g/kg, cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L; Improve again the seawater and freshwater volume recently regulate the water inlet salinity be 15g/kg, cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L and improves the seawater and freshwater volume again and recently regulate the water inlet salinity and be followed successively by 20g/kg, 25g/kg, 30g/kg, full seawater and respectively to each salinity cultured continuously to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L.From beginning to cultivate to the 389th day, reactor water inlet salinity reaches 15g/kg, water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 10mg/L, between domestication to NH ₄ ⁺-N and NO ₂ ^-The maximum material removal rate of-N all can reach 100%, and average removal rate is respectively 85.8% and 97.0%.When intaking is that full seawater and cultured continuously are to water outlet NH ₄ ⁺-N and NO ₂ ^-After-N concentration all was lower than 20mg/L, the anaerobic ammonia oxidation microbiological microbial film had been tamed to seawater and has been lived, and the startup of salt tolerant anammox reactor finishes.

Embodiment 2:

A. adopt existing anaerobic ammonia oxidation reactor, effective volume is 6L, and seed sludge is taken from the seed sludge of Dalian Ling Shui sewage work biochemistry pool, and the character of seed sludge: MLSS is 1.65g/L, and MLVSS is 0.687g/L.The reactor start-up condition: anaerobism, 33 ± 0.5 ℃ of temperature, HRT is 2 days, to change NH ₄Cl and NaNO ₃Dosage is regulated water inlet NH ₄ ⁺-N and NO ₂ ^--N concentration is 60～70mg/L.Monitor water outlet NH simultaneously ₄ ⁺-N and NO ₂ ^--N concentration was cultivated through 156 days, adhered to mycoderm on the reactor carrier and became sorrel by black, water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 10mg/L;

B. improve water inlet NH ₄ ⁺-N and NO ₂ ^--N concentration is 100～160mg/L, and regulating HRT is 1 day, and cultured continuously 50 days with the earlier quantitative sea water salinity of salometer, is added seawater again in water inlet, and recently regulating the water inlet salinity with the seawater and freshwater volume is 5g/kg, and cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L; Improve again the seawater and freshwater volume recently regulate the water inlet salinity be 10g/kg, cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L; Improve again the seawater and freshwater volume recently regulate the water inlet salinity be 15g/kg, cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L, improve the seawater and freshwater volume again and recently regulate the water inlet salinity and be followed successively by 20g/kg, 25g/kg, 30g/kg, full seawater and respectively to each salinity cultured continuously to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L.From beginning to be cultured to the 356th day, reactor water inlet salinity has reached 25g/kg, water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L.When intaking is that full seawater and cultured continuously are to water outlet NH ₄ ⁺-N and NO ₂ ^-After-N concentration all was lower than 20mg/L, the anaerobic ammonia oxidation microbiological microbial film had been tamed to seawater and has been lived, and the startup of salt tolerant anammox reactor finishes.

Claims (1)

1. the startup method of a salt tolerant anammox reactor is characterized in that carrying out as follows:

A. seed sludge is inserted in the anaerobic ammonia oxidation reactor, MLSS is that 1.65～69.12g/L, MLVSS are 0.687～21.33g/L in the seed sludge, operational conditions: anaerobism, and 33 ± 0.5 ℃ of temperature, HRT is 2 days, NH in the water inlet ₄ ⁺-N and NO ₂ ^--N concentration is 60～70mg/L, and cultured continuously is to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 10mg/L;

B. NH in will intaking ₄ ⁺-N and NO ₂ ^--N concentration all rises to 70～160mg/L, and HRT is 1～1.5 day, cultured continuously 20～50 days, regulate the water inlet salinity with seawater again and be followed successively by 5g/kg, 10g/kg, 15g/kg, 20g/kg, 25g/kg, 30g/kg, full seawater and respectively to each salinity cultured continuously to water outlet NH ₄ ⁺-N and NO ₂ ^--N concentration all is lower than 20mg/L.