CN102976489A - Method for starting anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater - Google Patents
Method for starting anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater Download PDFInfo
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Abstract
The invention discloses a method for starting an anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater. Anaerobic sludge and aerobic sludge are inoculated to a reactor, starting strategies are as follows: at first, anaerobic ammonium oxidation bacteria are enriched in the reactor through maintaining the conditions of anaerobism, organic carbon source-free feed water and no saltness, and a microflora system which takes the anaerobic ammonium oxidation bacteria as dominant bacteria is built; then, salt-tolerant anaerobic ammonium oxidation bacteria are preferentially selected under the conditions of low nitrogen concentration and low saltness, non-dominant bacteria are elutriated, and a microflora system which takes the salt-tolerant anaerobic ammonium oxidation bacteria as dominant bacteria is built; and finally, the nitrogen concentration and saltness are gradually increased, so that the ammonium oxidation bacteria can be suitable for high nitrogen concentration and high saltness environments. Through a mode of gradually changing a feed water base material, the problem of slow anaerobic ammonium oxidation bacteria enrichment under saltness inhibition is solved, the starting mode is simple and practicable, and the difficulty in starting a high-salt wastewater anaerobic ammonium oxidation treatment system is lowered.
Description
Technical field
The present invention relates to a kind of starting method of anaerobic ammonia oxidation reactor, to process the high salt high-nitrogen waste waters of industry such as chemical industry, food-processing, belong to technical field of waste water processing.
Background technology
Present high-salt wastewater total amount is huge and the trend that increases is year by year arranged, if unprocessed just direct discharging meeting causes severe contamination to environment.
Anammox is a kind of with NH
4 +-N is electron donor, with NO
2 --N is the microbial reaction that electron acceptor(EA) generates nitrogen, have good development prospect in the wastewater biological denitrificaion field, but anaerobic ammonia oxidizing bacteria is long generation time, poor growth.
The key that high-salt wastewater is processed is the domestication salt-durable microbe.High salinity can suppress microorganisms, so the enrichment of anaerobic ammonia oxidizing bacteria is slower under the hypersaline environment, causes utilizing the start time of Anammox reaction treatment high-salt wastewater system longer.In order to change this situation, there is application for a patent for invention 2010102266302 to disclose a kind of starting method of salt tolerant anammox reactor in the prior art, the method is first by improving nitrogen concentration, cultivate and the enrichment anaerobic ammonia oxidizing bacteria, after the question response device is realized the Anammox function, improve gradually again salinity, the domestication anaerobic ammonia oxidizing bacteria is to the adaptability of salinity, so start-up course can be divided into two processes of enrichment and domestication, the reactor start-up time is relatively long, and reactor is more weak to impact resistance.Yet in the actual production, envrionment conditions changes, and the unexpected variation of salinity and nitrogen concentration all can impact treatment system, so the method changing capability that conforms is inadequate.
Summary of the invention
For the prior art above shortcomings, the present invention proposes the starting method that a kind of anaerobic ammonia oxidation reactor is processed high salt nitrogenous effluent, and present method can improve the enrichment speed of anaerobic ammonia oxidizing bacteria under the hypersaline environment, shortens start time.
The present invention realizes that the technical solution of above-mentioned purpose is as follows:
Anaerobic ammonia oxidation reactor is processed the starting method of high salt nitrogenous effluent, it is characterized in that: carry out as follows:
1) at first anaerobic sludge and the aerobic sludge volume ratio with 3:1~1:1 is joined in the anaerobic ammonia oxidation reactor, inoculum size is 50%~70% of reactor useful volume; Reactor is incubated with lucifuge and processes;
2) ratio of control water inlet nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, and initial influent ammonium concentration is 28~56mg/L.Do not contain under the condition of organic carbon source and salinity in reactor anaerobism, water inlet, the operation reactor is until aerobic microbiological and heterotrophic microorganism death in the reactor forms the microflora take anaerobic ammonia oxidizing bacteria as dominant bacteria this moment;
3) the 2nd) step the basis on, beginning increases the water inlet salinity and is controlled in the 3g/L, be not suitable for the anaerobic ammonia oxidizing bacteria of surviving to eliminate under salinity, the operation reactor is with further optimization microflora, until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
4) improve water inlet nitrite nitrogen and ammonia nitrogen concentration on the basis of back, the ammonia nitrogen concentration lifting capacity is 25mg/L~40mg/L, nitrite nitrogen concentration lifting capacity is 25~60mg/L, and the ratio of nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, the operation reactor is until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
5) improve the water inlet salinity on the basis of back, water inlet salinity lifting capacity is 1.0g/L~2.5g/L, and the operation reactor is until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
6) constantly repeat the 4th) step and the 5th) step, alternately to improve water inlet nitrogen concentration and salinity, make the anaerobic ammonia oxidizing bacteria after the enrichment adapt to gradually high nitrogen concentration and high salinity environment, until water inlet nitrogen concentration and salinity reach nitrogen concentration and the salinity of pending waste water, namely finish the startup of anaerobic ammonia oxidation reactor.
The reactor operational conditions is controlled to be all the time: pH value 7.5~8.2,30~35 ℃ of temperature, hydraulic detention time 24~36h.
The present invention has solved under salinity suppresses slowly problem of anaerobic ammonoxidation bacterium concentrating by the mode that changes gradually water inlet matrix, improved the enrichment speed of anaerobic ammonia oxidizing bacteria under the hypersaline environment, Starting mode is simple, has reduced the difficulty that high-salt wastewater Anammox treatment system starts.
After present method successfully started anaerobic ammonia oxidation reactor, reactor was stable, and the denitrogenation of waste water rate is high.
Seed sludge of the present invention is the mixture of anaerobic sludge and aerobic sludge, microbe species is abundant, and the mode of taking salinity and nitrogen concentration alternately to improve, enrichment anaerobic ammonia oxidizing bacteria and domestication salt tolerant anaerobic ammonia oxidizing bacteria carry out synchronously, and the ability that anti-salinity and nitrogen concentration impact is more intense.Starting mode of the present invention more has superiority more near actual environment.
Embodiment
The present invention is inoculated in the reactor take anaerobic sludge and aerobic sludge as kind of a mud, utilizes the diversity of two kinds of sludge microbes, realizes the startup of Anammox in processing the high-salt wastewater reactor by the mode that changes gradually water inlet matrix.It is as follows to start strategy: at first keep anaerobism, water inlet not to contain enrichment anaerobic ammonia oxidizing bacteria under the condition of organic carbon source, non-saltness at reactor, set up the microflora take anaerobic ammonia oxidizing bacteria as dominant bacteria; Then preferred salt tolerant anaerobic ammonia oxidizing bacteria under the condition of low nitrogen concentration, Low-salinity is eluriated out non-dominant bacteria, sets up the microflora take the salt tolerant anaerobic ammonia oxidizing bacteria as dominant bacteria; Progressively improving at last nitrogen concentration and salinity makes anaerobic ammonia oxidizing bacteria adapt to high nitrogen concentration and hypersaline environment.Concrete steps are as follows:
1) at first anaerobic sludge and the aerobic sludge volume ratio with 3:1~1:1 is joined in the anaerobic ammonia oxidation reactor, inoculum size is 50%~70% of reactor useful volume; The reactor outside utilizes muff to be incubated and lucifuge.
2) ratio of control water inlet nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, and initial influent ammonium concentration is 28~56mg/L.Do not contain under the condition of organic carbon source and salinity in reactor anaerobism, water inlet, the operation reactor after the endogenous respiration and death of reactor experience aerobic microbiological, heterotrophic microorganism, forms the microflora take anaerobic ammonia oxidizing bacteria as dominant bacteria this moment.
3) the 2nd) step the basis on, beginning increases the water inlet salinity and is controlled in the 3g/L, be not suitable for the anaerobic ammonia oxidizing bacteria of surviving to eliminate under salinity, the operation reactor is with further optimization microflora, until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously.
4) improve water inlet nitrite nitrogen and ammonia nitrogen concentration on the basis of back, the ammonia nitrogen concentration lifting capacity is 25mg/L~40mg/L, nitrite nitrogen concentration lifting capacity is 25~60mg/L, and the ratio of nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, the operation reactor, until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously, show the successful enrichment salt tolerant of reactor anaerobic ammonia oxidizing bacteria this moment.
5) improve the water inlet salinity on the basis of back, water inlet salinity lifting capacity is 1.0g/L~2.5g/L, and the operation reactor is until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously.
6) constantly repeat the 4th) step and the 5th) step, alternately to improve water inlet nitrogen concentration and salinity, make the anaerobic ammonia oxidizing bacteria after the enrichment adapt to gradually high nitrogen concentration and high salinity environment, until water inlet nitrogen concentration and salinity reach nitrogen concentration and the salinity of pending waste water, namely finish the startup of anaerobic ammonia oxidation reactor.
The reactor operational conditions is controlled to be all the time: pH value 7.5~8.2,30~35 ℃ of temperature, hydraulic detention time 24~36h.
Below with a specific embodiment present method is described further.
Present embodiment is to carry out in the UASB reactor that a useful volume is 12L.Twine with muff the reactor outside, in order to insulation and lucifuge.Temperature maintains 32 ± 1 ℃.
1, at first anaerobically digested sludge and the aerobic sludge volume ratio with 2:1 is joined in the reactor, the seed sludge volume is 7.0L, is about 60% of reactor useful volume.Anaerobic sludge MLSS and MLVSS are respectively 6.05 g/L and 3.20 g/L, and aerobic sludge MLSS and MLVSS are respectively 0.798 g/L and 0.502 g/L.
2, regulating water inlet pH value is 8.0 ± 0.1, salinity is zero, nitrite nitrogen concentration and ammonia nitrogen concentration are respectively 36.40mg/L and 28.00mg/L, its ratio is about 1.30, reactor waterpower residence time 24h, through the continuous operation of 50d, total nitrogen, ammonia nitrogen, nitrite nitrogen clearance reach 80%~90%, the mud major part presents sorrel in the reactor, shows enrichment anaerobic ammonia oxidizing bacteria of reactor.
3, further preferred salt tolerant anaerobic ammonia oxidizing bacteria, the pH value remains unchanged, and nitrite nitrogen concentration and ammonia nitrogen concentration are brought up to 72.80mg/L and 56.00mg/L, and reactor is with 1.5 g/L and two salinity gradient operations of 3.0g/L.Behind the 60d, reactor total nitrogen, ammonia nitrogen, nitrite nitrogen clearance reach 80%~90%, show that preliminary screening goes out the salt tolerant anaerobic ammonia oxidizing bacteria, and the system microorganism has begun to adapt to the salinity environment.This stage, reactor performance obviously worsened after water inlet has just begun to add salinity, then slowly recovered also progressively to adapt to low-salt environment.
4, further divide gradient alternately to improve water inlet nitrogen load and salinity, nitrite nitrogen and ammonia nitrogen concentration improve gradient and are respectively 36.40mg/L and 28.00mg/L, and it is 1.5g/L that salinity improves gradient.After the 80d operation, water inlet nitrite nitrogen and ammonia nitrogen concentration rise to 145.6mg/L and 112mg/L, salinity reaches 15g/L, reach nitrogen concentration and the salinity of pending waste water, total nitrogen, ammonia nitrogen and nitrite nitrogen clearance all reach more than 80%, halophilic anaerobic ammonia oxidizing bacteria occupies main body and activity is higher in the microorganism species, and system starts successfully.This stage is alternately improved nitrogen load and salinity, and the reactor performance performance is stable, and the salinity lifting does not produce obviously impact to the activity of anaerobic ammonia oxidizing bacteria.
The present invention is take Sewage Plant anaerobic sludge and aerobic sludge as seed sludge, intake as reactor take the ratio of nitrite nitrogen concentration and ammonia nitrogen concentration as the artificial distribution of 1.2:1~1.4:1, in the reactor anaerobism, the water inlet low nitrogen concentration, do not contain enrichment anaerobic ammonia oxidizing bacteria under organic carbon source and the salinity environment.Then utilize the metabolism diversity of microorganism and environment to the selective power of microorganism, by changing gradually water inlet matrix, minute gradient improves the mode of nitrogen concentration and salinity, optimizes the salt tolerant anaerobic ammonia oxidizing bacteria.In the anaerobic ammonia oxidation reactor start-up course, the condition that finishes as each optimizing phase with the clearance of ammonia nitrogen and nitrite nitrogen, namely how long pipe reactor does not move, when nitrite nitrogen and ammonia nitrogen removal frank reach 80% when above simultaneously, some concentration promotes optimizing process and namely finishes, and enters next concentration again and promotes optimizing process.In anaerobic ammonia oxidation reactor Start-up and operating performance process, with the clearance of total nitrogen, ammonia nitrogen, nitrite nitrogen, the ratio growth activity of the ratio of nitrite nitrogen, ammonia nitrogen removal amount and anaerobic ammonia oxidizing bacteria is as the evaluation index of reactor operating performance.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (2)
1. anaerobic ammonia oxidation reactor is processed the starting method of high salt nitrogenous effluent, it is characterized in that: carry out as follows:
1) at first anaerobic sludge and the aerobic sludge volume ratio with 3:1~1:1 is joined in the anaerobic ammonia oxidation reactor, inoculum size is 50%~70% of reactor useful volume; Reactor is incubated with lucifuge and processes;
2) ratio of control water inlet nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, and initial influent ammonium concentration is 28~56mg/L;
Do not contain under the condition of organic carbon source and salinity in reactor anaerobism, water inlet, the operation reactor is until aerobic microbiological and heterotrophic microorganism death in the reactor forms the microflora take anaerobic ammonia oxidizing bacteria as dominant bacteria this moment;
3) the 2nd) step the basis on, beginning increases the water inlet salinity and is controlled in the 3g/L, be not suitable for the anaerobic ammonia oxidizing bacteria of surviving to eliminate under salinity, the operation reactor is with further optimization microflora, until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
4) improve water inlet nitrite nitrogen and ammonia nitrogen concentration on the basis of back, the ammonia nitrogen concentration lifting capacity is 25mg/L~40mg/L, nitrite nitrogen concentration lifting capacity is 25~60mg/L, and the ratio of nitrite nitrogen and ammonia nitrogen is 1.2:1~1.4:1, the operation reactor is until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
5) improve the water inlet salinity on the basis of back, water inlet salinity lifting capacity is 1.0g/L~2.5g/L, and the operation reactor is until nitrite nitrogen and ammonia nitrogen removal frank reach more than 80% simultaneously;
6) constantly repeat the 4th) step and the 5th) step, alternately to improve water inlet nitrogen concentration and salinity, make the anaerobic ammonia oxidizing bacteria after the enrichment adapt to gradually high nitrogen concentration and high salinity environment, until water inlet nitrogen concentration and salinity reach nitrogen concentration and the salinity of pending waste water, namely finish the startup of anaerobic ammonia oxidation reactor.
2. anaerobic ammonia oxidation reactor according to claim 1 is processed the starting method of high salt nitrogenous effluent, it is characterized in that: the reactor operational conditions is controlled to be all the time: pH value 7.5~8.2,30~35 ℃ of temperature, hydraulic detention time 24~36h.
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CN103693807A (en) * | 2013-12-13 | 2014-04-02 | 重庆大学 | Combination method for treating pickled vegetable processing wastewater based on anaerobic ammonia oxidation |
CN103739178A (en) * | 2013-12-26 | 2014-04-23 | 中南大学 | Rapid starting method of anaerobic ammonia oxidation reactor for enhancing sludge hydrolysis |
CN103979678A (en) * | 2014-03-14 | 2014-08-13 | 杭州师范大学 | Operation method of anaerobic ammonia oxidation reactor for processing high-salt wastewater |
CN107236682A (en) * | 2017-05-03 | 2017-10-10 | 南阳师范学院 | The Enrichment culture method of anaerobic ammonium oxidation sludge strain |
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