CN108946942A - A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity - Google Patents

A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity Download PDF

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CN108946942A
CN108946942A CN201810791699.6A CN201810791699A CN108946942A CN 108946942 A CN108946942 A CN 108946942A CN 201810791699 A CN201810791699 A CN 201810791699A CN 108946942 A CN108946942 A CN 108946942A
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salinity
water
bacterium
sludge
reactor
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倪寿清
葛成浩
卜翠娜
王屿
崔兆杰
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Shandong University
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • C02F3/302Nitrification and denitrification treatment
    • C02F3/307Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/004Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/05Conductivity or salinity
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/14NH3-N
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/16Total nitrogen (tkN-N)

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of methods for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, comprising: adds sodium chloride into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water reaches 2-3%, obtains domestication water;Active SNAP process sludge is added after mixing to batch-type biofiltration reactor (SBBGR);And it is passed through domestication water, the inhibition to NOB bacterium activity and abundance is successfully realized under high-intensitive aeration and 2-3% salinity, the activity reinforcing and enrichment to AOB bacterium.Method of the invention can effectively change the activity and abundance of the microbial function bacterium in SNAP technique: the activity and abundance of NOB bacterium reduce, the activity of AOB bacterium is improved and its abundance greatly improves, the activity and abundance of NOB bacterium, which are effectively suppressed, under 3% salinity can make SNAP technique nitrogen removal performance more stable, thus the risk for effectively avoiding SNAP Process for Effluent nitrogen removal rate not high.

Description

A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity
Technical field
The present invention relates to a kind of methods for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, belong to micro- Biological culture field.
Background technique
In recent years, relative to traditional biological denitrification process, Novel sewage denitrification process: Anammox (Anammox) work Skill, which relies on without additional carbon, without exposing the advantages such as large quantity of air, capital construction is at low cost, becomes grinding for various countries' water environment protection field Study carefully hot spot.However, simple anaerobic ammonia oxidation process, which exists, needs the shortcomings that adding additional nitrite nitrogen.Short distance nitration and anaerobism ammonia Short distance nitration-anaerobic ammoxidation (SNAP) technique of oxidation coupling is on the basis of being not required to add additional nitrite nitrogen, Ji Keyou Ammonium nitrogen in the removal sewage of effect, theoretical reaction equation are as follows:
Although short distance nitration-anaerobic ammoxidation (SNAP) technique can effectively remove the ammonium nitrogen in sewage, by long-term Application experiment discovery, in SNAP technique, NOB and Anammox bacterium compete nitrite nitrogen, oxygen are competed with AOB, so as to cause SNAP Technique nitrogen removal rate is not high, and runing time is long, and it is fiercer that NOB and Anammox bacterium competes, and causes technique that cannot stablize fortune Row.Therefore, activity and enrichment pair that suitable condition promotes AOB on the basis of effectively inhibiting the activity and abundance of NOB are found The practical application of SNAP technique is of great significance.
Summary of the invention
In view of the deficiencies of the prior art, the present invention relates to one kind inhibits nitrite oxidizing bacteria enrichment culture ammonia based on salinity The method of oxidation bacteria.
Term explanation:
Salinity: salinity contributors are sodium chloride, in terms of sodium chloride, concentration 2-3%.
Technical scheme is as follows:
A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity, is comprised the following steps that
(1) sodium chloride is added into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water reaches 2-3%, obtains domestication water;
(2) active SNAP process sludge is added after mixing to batch-type biofiltration reactor (SBBGR);
(3) it is passed through the domestication water of step (1) from the bottom batch-type biofiltration reactor (SBBGR), controls water intake velocity Water speed out makes hydraulic detention time control in 40-55h;Temperature is adjusted, temperature is made to maintain 30-32 DEG C;Height is carried out simultaneously Intensity aeration, aeration time 230-250min, aeration intensity 40-50mL/min;
(4) service condition for keeping step (3), after stable operation 170-190 days, the successfully realization pair under 2-3% salinity The inhibition of NOB bacterium activity and abundance is strengthened and is enriched with to the activity of AOB bacterium.
Preferred according to the present invention, in step (1), taming ammonia nitrogen concentration in water is 185 ± 5mg/L.
Preferred according to the present invention, in step (1), the salinity for taming water is 2.8-3.0%.
Preferred according to the present invention, in step (2), active SNAP process sludge schedules to last the nitration-anaerobic ammoxidation run The sludge generated is reacted, operation nitrogen load is 0.1-0.3kg N/m3/d;Sludge is in brownish black, granularity 0.5- 2mm, moisture percentage in sewage sludge are more than or equal to 98wt%.
Active SNAP process sludge of the invention is derived from a kind of quick start of Chinese patent literature CN201510771588.5 Reactor successfully starts up after running 50~110 days in the reactor and quick start method of Anammox coupling nitrosation reaction Sludge anaerobic ammoxidation couple nitrosation reaction generate SNAP process particulates sludge, then filtered, rinse after obtain.
It is preferred according to the present invention, in step (2), the dosage of active SNAP process sludge: so that reactor be passed through it is tame and docile Changing sludge concentration when water operates normally is 6000 ± 50mg/L.
Preferred according to the present invention, in step (3), aeration time 240min, aeration intensity 40mL/min are aerated gas Body is air.
Preferred according to the present invention, the batch-type biofiltration reactor includes double layer sleeve barrel cylindrical reactor, The bottom of reactor is provided with water inlet, side wall upper part is provided with water outlet, and inner cylinder is reaction zone, is between inner cylinder and outer cylinder Hot-water cavity is enclosed with outside outer cylinder for heating and is protected from light paper, provide good growing environment for anammox bacterium and AOB bacterium.
It is further preferred that the reaction zone includes upper layer aeration zone and filler sludge area of lower layer, upper layer aeration zone with Filler sludge area of lower layer is snapped together by bolt seal;Filler sludge area of lower layer bottom is equipped with the gravel with a thickness of 3~5cm Rock layers, gravel layer cobble-stone average diameter are 2-8mm, the water inlet pipe being connected with water inlet are equipped in gravel layer, in gravel Layer top is provided with water distributing plate with holes, and packing layer is provided on water distributing plate, there is gap, the hole of packing layer inside packing layer Rate be 0.6~0.8, packing layer with a thickness of the 2/5~1/2 of height for reactor.
It is further preferred that the packing layer is formed by several inside polyethylene carrier particle packing with holes, gather The specific surface area of ethylene filler particles: 680-710m2/m3
In order to be uniformly mixed sewage in reactor, sewage internal circulation apparatus is arranged in reactor, and circulation revolution speed control exists 60rpm。
It is preferred according to the present invention, it is provided with gas-guide tube at the top of the batch-type biofiltration reactor, gas-guide tube is set There is water seal, water outlet connects outlet pipe, and outlet pipe can be maintained by time switch and solenoid valve control in anaerobism section starvation Anaerobic environment.
Inventor has been surprisingly found that, by the way that sodium chloride is added into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water is 2-3%, by the way that aeration is tamed by force in batch-type biofiltration reactor, so that SNAP technique Bacterial community greatly changes Become, NOB activity inhibited and the reduction of its abundance in SNAP technique, AOB bacterium activity improves and its abundance increases;Improve SNAP work The stability of skill, greatly improves nitrogen removal rate.
Currently preferred salinity source is sodium chloride, is a kind of non-toxic and tasteless, and often come across in industrial wastewater Substance.
The present invention is using the relevant flora of nitrogen cycle in salinity regulation SNAP technique, so that the SNAP activated sludge after domestication The activity and abundance of middle NOB bacterium substantially reduce, and strengthen the activity of AOB bacterium and improve its abundance.
The present invention has the advantage that
1, method of the invention can change SNAP technique Bacterial community so that in SNAP technique NOB activity inhibited and Its abundance reduces, and AOB bacterium activity improves and its abundance increases: when water inlet total nitrogen volumetric loading is in 0.1Kg N/m3When/d or so, The SOUR value for being not added with NOB bacterium in the reactor of salinity is 0.016mg O2/ (g MLSS min), and the known NOB found The abundance of Pseudomonas Nitrospira is 2.82%, and the SOUR value of the AOB bacterium in reactor is 0.049mg O2/(g MLSS Min), the abundance of AOB Cordycepps Nitrosomonadaceae be 0.35%, AOB Pseudomonas Nitrosomonas and Nitrosovibrio abundance is respectively 0%;When adding salinity, the SOUR value of NOB bacterium is in reactor under enhanced aeration 0.004mg O2/ (g MLSS min), and the abundance of NOB Pseudomonas Nitrospira is for the SOUR value of 1.59%, AOB bacterium 0.059mg O2The abundance of/(g MLSS min), AOB Cordycepps Nitrosomonadaceae are 1.88%, AOB Pseudomonas Nitrosomonas and Nitrosovibrio abundance is respectively 0.03% and 0.15%;As shown in table 1.
Table 1 adds the reactor of salinity and is not added with the activity change situation of AOB bacterium and NOB bacterium in the reactor of salinity
2, the present invention is right by having successfully started up the inhibition to nitrite oxidizing bacteria under high salinity and strong aeration condition The enrichment culture of ammonia oxidation bacteria.
3, method service condition of the invention is mild, can be carried out at normal temperatures and pressures, is not necessarily to specified conditions;And nothing has Malicious harmful substance generates, environmentally safe harmless;
Detailed description of the invention
Fig. 1 be embodiment 1 method and comparative example 1 method to biological community structure section's level situation of change Figure;
Fig. 2 is that the method for embodiment 1 and the method for comparative example 1 are belonging to horizontal situation of change to biological community structure Figure.
Specific embodiment
Below by embodiment and referring to attached drawing, the present invention is described in detail.It is important to point out that embodiment is served only for Invention is further explained, should not be understood as limiting the scope of the invention, the person skilled in the art in the field Some nonessential modifications and adaptations can be made to the present invention according to aforementioned present invention content.
It is bought in Sinopharm Chemical Reagent Co., Ltd. in embodiment using chemical reagent, is that analysis is pure.
SBBGR reactor is made of polymethyl methacrylate materials.
Aeration ingredient is air.
Batch-type biofiltration reactor in embodiment includes double layer sleeve barrel cylindrical reactor, in the bottom of reactor It is provided with water inlet, side wall upper part is provided with water outlet, and inner cylinder is reaction zone, and reaction zone includes upper layer aeration zone and lower layer's filler Sludge area, upper layer aeration zone are snapped together with filler sludge area of lower layer by bolt seal;Lower layer filler sludge area bottom paving Equipped with the gravel layer with a thickness of 3cm, gravel layer cobble-stone average diameter is 6mm, is equipped in gravel layer and is connected with water inlet Water inlet pipe, water distributing plate with holes is provided with above gravel layer, is provided with packing layer on water distributing plate, it is free inside packing layer Gap, the porosity of packing layer are 0.6~0.8, packing layer with a thickness of the 1/2 of height for reactor.Packing layer is by several inside Polyethylene carrier particle packing with holes is formed, the specific surface area of polyethylene carrier particle: 710m2/m3
It is that hot-water cavity is enclosed with outside outer cylinder for heating and is protected from light paper between inner cylinder and outer cylinder, is anammox bacterium and AOB Bacterium provides good growing environment.
In order to be uniformly mixed sewage in reactor, sewage internal circulation apparatus is arranged in reactor, and circulation revolution speed control exists 60rpm。
It is preferred according to the present invention, it is provided with gas-guide tube at the top of the batch-type biofiltration reactor, gas-guide tube is set There is water seal, water outlet connects outlet pipe, and outlet pipe can be maintained by time switch and solenoid valve control in anaerobism section starvation Anaerobic environment.
Embodiment 1
A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity, is comprised the following steps that
(1) sodium chloride is added into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water reaches 3%, is obtained domestication water, is tamed water Middle ammonia nitrogen concentration is 185 ± 5mg/L;
(2) active SNAP process sludge is added after mixing to batch-type biofiltration reactor (SBBGR), it is living Property SNAP process sludge schedule to last run nitration-anaerobic ammoxidation reaction generate sludge, operation nitrogen load be 0.1kgN/m3/ d;Sludge is in brownish black, and granularity 1mm, moisture percentage in sewage sludge is more than or equal to 99wt%.
(3) it is passed through the domestication water of step (1) from the bottom batch-type biofiltration reactor (SBBGR), controls water intake velocity Water speed out makes hydraulic detention time control in 48h;Temperature is adjusted, temperature is made to maintain 31 DEG C;High-intensitive exposure is carried out simultaneously Gas, aerating gas are air, aeration time 240min, aeration intensity 40mL/min;
(4) service condition for keeping step (3) after stable operation 189 days, is successfully realized living to NOB bacterium under 3% salinity Property and abundance inhibition, to AOB bacterium activity strengthen and enrichment.
Comparative example 1
A kind of method of autotrophy culture AOB and NOB, comprises the following steps that
(1) active SNAP process sludge is uniformly mixed and is added to batch-type biofiltration reactor, mixing sludge contains Water rate >=99wt%;The batch-type biofiltration sidewall of reactor be equipped with different height sample tap, bottom have water distributing plate and Effluent cycle system guarantees that aeration zone and reaction zone sewage uniformly mix in reactor;Active SNAP process sludge schedules to last operation The sludge that nitration-anaerobic ammoxidation reaction generates, operation nitrogen load are 0.1kg N/m3/d;Sludge is in brownish black, granule sludge Partial size is 1mm, and moisture percentage in sewage sludge is more than or equal to 99wt%.
(2) bottom of batch-type biofiltration reactor is passed through the waste water to be processed of not sodium chloride-containing using peristaltic pump, leads to Time switch and wriggling revolution speed control water inlet total amount are crossed, hydraulic detention time 48h in reactor is made.Heating rod is used simultaneously Inside reactor temperature is set to maintain 31 degree with water-bath circulator;Aeration phase is 240min, aeration intensity 20mL/min.
(3) keep batch-type biofiltration reactor according to successfully realizing autotrophic condition after above-mentioned condition stable operation 19 days The enrichment of lower AOB bacterium and NOB bacterium.
Application experiment example:
To being passed through respectively required artificial synthesized waste water in the method for embodiment 1 and comparative example 1, time switch and compacted is utilized Dynamic pump control water inlet total amount, is discharged using time switch and solenoid valve control, makes hydraulic detention time 48 hours;It is described artificial The specific ingredient of synthetic wastewater are as follows: 1044mg NH4HCO3/ L, 30g NaCl/L (comparative example 0), 1250mg KHCO3/ L, 25mg KH2PO4/ L, 200mg MgSO4·7H2O/L, 300mg CaCl2·2H2O/L, 1mL microelement/L.The microelement is specific Ingredient is 20000mg EDTA/L, 430mg ZnSO4·7H2O/L, 240mg CoCl2·6H2O/L, 990mg MnCl2·4H2O/ L, 250mg CuSO4·5H2O/L, 220mg NaMoO4·2H2O/L, 190mg NiCl2·6H2O/L, 210mg NaSeO4· 6H2O/L, 14mg H3BO4/L。
It runs and sludge is taken within the 189th day to carry out sludge specific oxygen utilization rate (SOUR) measurement;It runs and sludge sample is taken within the 189th day to carry out High-flux sequence, accordingly in assessing reactor biological community structure variation, the variation of biological community structure is as shown in Figure 1, Figure 2 It is shown.
After reactor in comparative example 1 is stable, the SOUR value of NOB bacterium is 0.016mg O2/ (g MLSS min), and It was found that the abundance of known NOB Pseudomonas Nitrospira be 2.82%, the SOUR value of the AOB bacterium in reactor is 0.049mg O2The abundance of/(g MLSS min), AOB Cordycepps Nitrosomonadaceae be 0.35%, AOB Pseudomonas Nitrosomonas and Nitrosovibrio abundance is respectively 0%;And the embodiment of the present invention 1 be added 3% salinity reactor it is stable after, NOB bacterium SOUR value be 0.004mg O2/ (g MLSS min), and the abundance of NOB Pseudomonas Nitrospira is 1.59%, AOB bacterium SOUR value is 0.059mg O2The abundance of/(g MLSS min), AOB Cordycepps Nitrosomonadaceae are 1.88%, AOB bacterium Belonging to Nitrosomonas and Nitrosovibrio abundance is respectively 0.03% and 0.15%.
Embodiment 2
A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity, is comprised the following steps that
(1) sodium chloride is added into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water reaches 2.8%, obtains domestication water, domestication Ammonia nitrogen concentration is 185 ± 5mg/L in water;
(2) active SNAP process sludge is added after mixing to batch-type biofiltration reactor (SBBGR), it is living Property SNAP process sludge schedule to last run nitration-anaerobic ammoxidation reaction generate sludge, operation nitrogen load be 0.3kgN/m3/ d;Sludge is in brownish black, and granularity 1.5mm, moisture percentage in sewage sludge is more than or equal to 99wt%.
(3) it is passed through the domestication water of step (1) from the bottom batch-type biofiltration reactor (SBBGR), controls water intake velocity Water speed out makes hydraulic detention time control in 48h;Temperature is adjusted, temperature is made to maintain 31 DEG C;High-intensitive exposure is carried out simultaneously Gas, aerating gas are air, aeration time 240min, aeration intensity 45mL/min;
(4) service condition of step (3) is kept successfully to realize under 2.8% salinity to NOB bacterium after stable operation 180 days The inhibition of activity and abundance is strengthened and is enriched with to the activity of AOB bacterium.

Claims (9)

1. a kind of method for being inhibited nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, is comprised the following steps that
(1) sodium chloride is added into ammonia nitrogen waste water, so that the salinity of ammonia nitrogen waste water reaches 2-3%, obtains domestication water;
(2) active SNAP process sludge is added after mixing to batch-type biofiltration reactor (SBBGR);
(3) it is passed through the domestication water of step (1) from the bottom batch-type biofiltration reactor (SBBGR), control water intake velocity and goes out Water speed makes hydraulic detention time control in 40-55h;Temperature is adjusted, temperature is made to maintain 30-32 DEG C;High intensity is carried out simultaneously Aeration, aeration time 230-250min, aeration intensity 40-50mL/min;
(4) service condition for keeping step (3), after stable operation 170-190 days, is successfully realized under 2-3% salinity to NOB bacterium The inhibition of activity and abundance is strengthened and is enriched with to the activity of AOB bacterium.
2. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, in step (1), taming ammonia nitrogen concentration in water is 185 ± 5mg/L.
3. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, in step (1), the salinity for taming water is 2.8-3.0%.
4. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, in step (2), active SNAP process sludge schedules to last the sludge that the nitration-anaerobic ammoxidation reaction run generates, fortune Row nitrogen load is 0.1-0.3kg N/m3/d;Sludge is greater than in brownish black, granularity 0.5-2mm, moisture percentage in sewage sludge Equal to 98wt%.
5. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, in step (2), the dosage of active SNAP process sludge: so that reactor is passed through dirt when domestication water operates normally Mud concentration is 6000 ± 50mg/L.
6. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, in step (3), aeration time 240min, aeration intensity 40mL/min, aerating gas is air.
7. the method according to claim 1 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, the batch-type biofiltration reactor includes double layer sleeve barrel cylindrical reactor, is set in the bottom of reactor It is equipped with water inlet, it is hot-water cavity between inner cylinder and outer cylinder that side wall upper part, which is provided with water outlet, and inner cylinder is reaction zone, for heating, It is enclosed with outside outer cylinder and is protected from light paper, provide good growing environment for anammox bacterium and AOB bacterium.
8. the method according to claim 7 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, the reaction zone includes upper layer aeration zone and filler sludge area of lower layer, upper layer aeration zone and lower layer's filler sludge Area is snapped together by bolt seal;Filler sludge area of lower layer bottom is equipped with the gravel layer with a thickness of 3~5cm, gravel layer Cobble-stone average diameter is 2-8mm, and the water inlet pipe being connected with water inlet is equipped in gravel layer, is provided with above gravel layer Water distributing plate with holes is provided with packing layer on water distributing plate, there is gap inside packing layer, and the porosity of packing layer is 0.6~ 0.8, packing layer with a thickness of the 2/5~1/2 of height for reactor.
9. the method according to claim 7 for inhibiting nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria based on salinity, It is characterized in that, the packing layer is formed by several inside polyethylene carrier particle packing with holes, polyethylene carrier particle Specific surface area: 680-710m2/m3
CN201810791699.6A 2018-07-18 2018-07-18 A method of nitrite oxidizing bacteria enrichment culture ammonia oxidation bacteria is inhibited based on salinity Pending CN108946942A (en)

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CN110127955A (en) * 2019-06-12 2019-08-16 成都工业学院 A kind of double pond manual controlled infusions and method for low C/N than wastewater efficient denitrogenation

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KR101861099B1 (en) * 2017-10-25 2018-05-28 주식회사 부강테크 Apparatus and method for culture of candidatus brocadia sinica oba1 using partial nitritation sequencing batch reactor supplied with ammonium oxidation bacteria(aob) granule from the connected aob granule reactor and using anaerobic ammonium oxidation reactor

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Publication number Priority date Publication date Assignee Title
CN102381803A (en) * 2010-09-06 2012-03-21 中国科学院城市环境研究所 Method for starting full-flow autotrophic nitrogen removal process by taking general activated sludge as seed sludge
CN102976489A (en) * 2012-12-21 2013-03-20 重庆大学 Method for starting anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater
KR101861099B1 (en) * 2017-10-25 2018-05-28 주식회사 부강테크 Apparatus and method for culture of candidatus brocadia sinica oba1 using partial nitritation sequencing batch reactor supplied with ammonium oxidation bacteria(aob) granule from the connected aob granule reactor and using anaerobic ammonium oxidation reactor

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110127955A (en) * 2019-06-12 2019-08-16 成都工业学院 A kind of double pond manual controlled infusions and method for low C/N than wastewater efficient denitrogenation
CN110127955B (en) * 2019-06-12 2024-04-30 成都工业学院 Double-pond artificial rapid infiltration system and method for high-efficiency denitrification of low-C/N ratio sewage

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Application publication date: 20181207