CN101892180A - Corynebacterium humireducens and application thereof - Google Patents
Corynebacterium humireducens and application thereof Download PDFInfo
- Publication number
- CN101892180A CN101892180A CN2010101692143A CN201010169214A CN101892180A CN 101892180 A CN101892180 A CN 101892180A CN 2010101692143 A CN2010101692143 A CN 2010101692143A CN 201010169214 A CN201010169214 A CN 201010169214A CN 101892180 A CN101892180 A CN 101892180A
- Authority
- CN
- China
- Prior art keywords
- corynebacterium humireducens
- fuel cell
- humireducens
- microbe
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention discloses corynebacterium humireducens and an application thereof. The corynebacterium humireducens is obtained by separating and purifying anolyte of a sludge microbe fuel cell, and is preserved in the ordinary microbe centre of the China Microbe Culture Preservation Management Committee on April 14, 2008 with the preservation number of CGMCC No.2452. The corynebacterium humireducens has wide utilization spectrum of electron donor under the anaerobic condition, has efficient humus reduction capability and electrogenesis capability under the alkaline condition, can be widely applied to in-situ microbe restoration of organic pollutants, microbe fuel cell generating technology or recycling processing of high-concentration organic wastes.
Description
Technical field
The invention belongs to environment and technical field of new energies, be specifically related to a strain tool electrogenesis and active Corynebacterium humireducens of humus respiration and application thereof.
Technical background
Humus respiration is the novel microorganism energy metabolism mode that receives much attention in recent years.Humus respiration claims quinone to breathe again, is meant that microorganism is terminal electron acceptor with the soil ulmin, by the reduction of oxidation electron donor coupling soil ulmin, and stores the energy of vital movement from this process.The microorganism that can carry out humus respiration is called soil ulmin reduction bacterium.At present, the environment importance of humus respiration has been widely accepted and has admitted: humus respiration not only with the natural circulation process coupling mutually of elements such as carbon, nitrogen, phosphorus, but also remarkably influenced heavy metal and organic pollutant reduction transform.Soil ulmin reduction bacterium has good application prospects in the pollutent original position field of repairing, and the new soil ulmin reduction bacterium of separation and purification becomes studies focus in recent years.
(Microbial fuel cell is that to be catalyzer with the microorganism directly change into the device of electric energy with the chemical energy in the organism MFC) to microbiological fuel cell, has the double effects that electrogenesis and organic waste are disposed.The factor that influences electrogenesis efficient comprises microbial metabolic activity, sun/cathode material etc.Wherein, microorganism electricity generation ability is the greatest factor that influences the MFC output power density.
In recent years discover that part iron-reducing bacterium and soil ulmin reduction bacterium all has efficient electrogenesis activity, study maximum be Shiva Bordetella (Shewanella) and Bacillaceae (Geobacter).These bacterium mostly grow under neutrallty condition, and mostly are Gram-negative bacteria.Alkaline condition helps to improve electrogenesis efficient, but alkaline environment is unfavorable for most of microorganism growth, so this patent is intended to develop alkaline soil ulmin reduction and electrogenesis microorganism.Through document and patent retrieval, do not find under alkaline condition, to have the report of soil ulmin reduction and electrogenesis active bacterial strain as yet.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art, the soil ulmin reduction and the efficient bacterial strain of electrogenesis---the Corynebacterium humireducens (Corynebacterium humireducens) of growth under a kind of alkaline condition is provided.
Another object of the present invention provides the separation purification method of described Corynebacterium humireducens.
The application that also has a purpose to provide described Corynebacterium humireducens of the present invention.
The present invention screens the Corynebacterium humireducens (Corynebacteriumhumireducens) of gained, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica) on April 14th, 2008, preserving number is CGMCC 2452.
Described Corynebacterium humireducens has following form and physio-biochemical characteristics:
1) thalli morphology characteristic
Adopt conventional bacterium electron microscope observation, this bacterial strain is a gram-positive microorganism; Scanning electronic microscope result shows that bacterial strain is bar-shaped, and the thalline size is 0.5～0.7 μ m * 1.5～2.5 μ m, does not move.
2) colonial morphology characteristic
Aerobic cultivation is after 24 hours on beef extract-peptone agar solid medium, the bacterium colony yellow, and smooth surface, opaque, neat in edge, colony diameter is 2～3mm.
3) physiological and biochemical property
Salt tolerance is strong, can grow in the nutrient solution of 0～12% sodium-chlor (NaCl); Optimum growth temperature is 37 ℃, and the pH value of suitable growth is 8～9.Tool amphimicrobian energy for growth.The catalase positive, chemoheterotrophic bacteria, fermentating metabolism does not produce acid, and aerogenesis not can reduce nitrate.Under anaerobic, its electron donor utilization spectrum is wider, can be electron donor reduction AQDS with formic acid, acetate, propionic acid, lactic acid, ethanol or sucrose, can not utilize citric acid or glycerine.
4) molecular biological characteristics
Adopt the method for SDS-Proteinase K, chloroform-primary isoamyl alcohol (volume ratio 24: 1) extracting, 0.6 volume isopropanol precipitating to extract bacteria total DNA.Adopt the 16SrDNA of bacterial 16 S rDNA universal primer F8 and R1542 amplification bacterium, tangible band appears near 1500bp, to carry out sequencing after the pcr amplification product recovery, with the dna sequence dna input GenBank that obtains, with the Blastn program all sequences in the database is compared analysis, found that 6 nearest strain bacterium of bacterial strain homology of the present invention all belong to excellent bacillus, but similarity is defined as novel species all less than 97%.
The present invention provides the separation purification method of described Corynebacterium humireducens simultaneously: undertaken by the alkaline sludge anolyte to this laboratory microbiological fuel cell that separation and purification obtains.
Concrete separation purification method may further comprise the steps:
1) the mud anolyte of getting microbiological fuel cell is inoculated in the liquid separation culture medium;
The composition of liquid separation culture medium is: contain 1.0g glucose (electron donor), 16gAQDS (electron acceptor(EA)), 2.5g NaHCO in every liter of liquid separation culture medium
3, 0.25g NH
4Cl, 0.678g NaH
2PO
42H
2O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution, pH value are 10.Wherein, vitamin solution is to contain 2.0mg vitamin H, 2.0mg folic acid, 10.0mg vitamins B in every liter of deionized water
6, 5.0mg VitB1,5.0mg riboflavin, 5.0mg nicotinic acid, 5.0mg calcium pantothenate, 0.1mg vitamins B
12, 5.0mg para-amino benzoic acid, 5.0mg Thioctic Acid; Trace element solution is to contain 1.5g nitrilotriacetic acid(NTA), 3.0g MgSO in every liter of deionized water
47H
2O, 0.5g MnSO
4H
2O, 1.0g NaCl, 0.1g FeSO
47H
2O, 0.1g CoCl
26H
2O, 0.1g CaCl
2, 0.1g ZnSO
47H
20,0.01g CuSO
45H
2O, 0.01g AlK (SO
4)
212H
2O, 0.01g H
3BO
3, 0.01g Na
2MoO
42H
2O.
2) drum fills high pure nitrogen and is no less than 30 minutes in the liquid separation culture medium of the anolyte of seed sludge microbiological fuel cell, and air-blowing finishes and cover serum cap and add aluminium lid and seal, and places anaerobism workstation (N
2And CO
2Mixing according to 80: 20 volume ratios), 30 ℃ leave standstill cultivation, observe the colour-change situation of nutrient solution.
3) when the nutrient solution color from colourless when becoming deep yellow, the inoculum size with 10% is forwarded to another fresh liquid separation culture medium with nutrient solution, anaerobic operation and anaerobism culture condition are with (2).So enrichment culture is three times.
4) reacted nutrient solution employing of the 4th generation dilution-plate method is diluted to 10
5～10
6Individual/mL, the nutrient solution 0.1mL after the dilution is evenly coated on the LB solid medium, place anaerobism workstation (N
2/ CO
2=80/20) cultivate 48h for 30 ℃, picking list bacterium colony carries out isolation and purification.The composition of described LB solid medium is: peptone 10g/L yeast extract 5g/L, and NaCl 10g/L, agar powder 20g/L, the pH value is 10.
Corynebacterium humireducens of the present invention electron donor utilization spectrum under anaerobic is wider, can be electron donor reduction AQDS with formic acid, acetate, propionic acid, lactic acid, ethanol or sucrose, can be widely used in the biology in situ reparation of organic pollutant.
Have soil ulmin reducing power and electrogenesis activity efficiently under the Corynebacterium humireducens alkaline condition of the present invention, its electrogenesis under alkaline condition is stable, fuel utilization spectrum is wider, can utilize multiple organism such as lactic acid, glucose, sucrose, starch, Succinic Acid, maltose or wood sugar, can be used for the disposal of resources of microbiological fuel cell generation technology and high density organic waste.
The invention has the beneficial effects as follows:
The invention provides a kind of new soil ulmin that under alkaline condition, has and reduce and the active coryneform bacterial strains of electrogenesis,, have a extensive future for soil ulmin reduction and microbiological fuel cell generation technology under the alkaline condition provide a kind of new high-effective microorganism bacterial strain.
Description of drawings
Fig. 1 is the stereoscan photograph of Corynebacterium humireducens CGMCC 2452 of the present invention under 20000 times
Fig. 2 is the phylogenetic tree of Corynebacterium humireducens CGMCC 2452
Fig. 3 for Corynebacterium humireducens CGMCC 2452 pH be 9 o'clock the reduction AQDS electronics utilize situation
Fig. 4 is for utilizing the electrogenesis activity of different substrate correspondences under Corynebacterium humireducens CGMCC 2452 alkaline conditions
Embodiment
Describe the present invention in detail below in conjunction with the drawings and specific embodiments.Testing used lactic acid, acetate, formic acid, propionic acid, ethanol, sucrose or glucose etc. does not give unnecessary details one by one in an embodiment.But therefore do not limit the scope of the invention.
Embodiment 1: the separation and purification of Corynebacterium humireducens of the present invention
1) getting the 5mL anolyte from pH is 10 sludge microbe fuel cell is inoculated in the 100mL liquid separation culture medium.
Done detailed description in the patent application that is configured in number of patent application 200910041235.4 of described sludge microbe fuel cell.
The composition of described liquid separation culture medium is: contain 1.0g glucose (electron donor), 16g AQDS (electron acceptor(EA)), 2.5g NaHCO in every liter of liquid separation culture medium
3, 0.25gNH
4Cl, 0.678g NaH
2PO
42H
2O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution, pH value are 10.Wherein, vitamin solution is to contain 2.0mg vitamin H, 2.0mg folic acid, 10.0mg vitamins B in every liter of deionized water
6, 5.0mg VitB1,5.0mg riboflavin, 5.0mg nicotinic acid, 5.0mg calcium pantothenate, 0.1mg vitamins B
12, 5.0mg para-amino benzoic acid, 5.0mg Thioctic Acid; Trace element solution is to contain 1.5g nitrilotriacetic acid(NTA), 3.0g MgSO in every liter of deionized water
47H
2O, 0.5g MnSO
4H
2O, 1.0g NaCl, 0.1gFeSO
47H
2O, 0.1g CoCl
26H
2O, 0.1g CaCl
2, 0.1g ZnSO
47H
2O, 0.01gCuSO
45H
2O, 0.01g AlK (SO
4)
212H
2O, 0.01g H
3BO
3, 0.01gNa
2MoO
42H
2O.
2) drum fills high-purity mixed gas and is no less than 30 minutes in the liquid separation culture medium of the anolyte of seed sludge microbiological fuel cell, and described high-purity mixed gas can adopt N
2And CO
2According to the mixed gas of 80: 20 volume ratios, air-blowing finishes with the aluminium lid sealing, places anaerobism workstation (N
2/ CO
2=80/20), 30 ℃ leave standstill cultivation, observe the colour-change situation of nutrient solution.
3) when the nutrient solution color from colourless when becoming deep yellow, inoculum size with 10% is forwarded to another fresh liquid separation culture medium with nutrient solution, drum fills high-purity mixed gas and is no less than 30 minutes, and air-blowing finishes with the aluminium lid sealing, places the anaerobism workstation to leave standstill cultivation for 30 ℃.So enrichment culture is three times.
4) reacted nutrient solution employing of the 4th generation dilution-plate method is diluted to 10
-5～10
-6, the nutrient solution 0.1mL after the dilution is evenly coated on the LB solid medium, place anaerobism workstation (N
2/ CO
2=80/20) cultivates 48h for 30 ℃, picking list bacterium colony purifying, promptly get described bacterial strain (Corynebacterium humireducens), be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica) on April 14th, 2008, preserving number is CGMCC 2452.
The composition of described LB solid medium is: peptone 10g/L, and yeast extract 5g/L, NaCl 10g/L, agar powder 20g/L, the pH value is 10.
Embodiment 2: the physio-biochemical characteristics of bacterial strain (Corynebacterium humireducens)
Bacterial strain is non-fermented type facultative anaerobe, salt tolerant alkali.Growth temperature range is 25～40 ℃, reaches more than 45 ℃ and can not grow below 10 ℃; Growth pH scope is 7.0～11.0 (the suitableeest: pH9.0); The required NaCl% that grows is 0～12% (＞13% can not grow).According to physio-biochemical characteristics, be excellent bacillus (Corynebacteria) with this dientification of bacteria.
The physiological and biochemical property of table 1 invention bacterial strain
+ expression is positive;-expression the reaction that is negative
Embodiment 3: the molecular classification status of bacterial strain (Corynebacterium humireducens)
As shown in Figure 2, the 6 strain bacterium nearest with invention bacterial strain homology all belong to excellent bacillus, be respectively: C.marinum (similarity 96.9%, 1393bp), C.testudinoris (96.8%, 1395bp), C.halotolerans (96.4%, 1392bp), C.felinum (96.0%, 1383bp), C.singulare (96.0%, 1297bp) and C.freiburgense (95.9%, 1395bp).According to the phylogeny theory, similarity just can be thought identical bacterial strain only greater than 97% between bacterial strain, and the similarity of the most close bacterial strain C.marinum with it of aimed strain also has only 96.9%, so this Mycota is decided to be novel species.
In conjunction with above-mentioned physio-biochemical characteristics, 16SrRNA sequence alignment result, Corynebacterium humireducens of the present invention belongs to Corynebacterium, for this belongs to the novel species of bacterium, called after Corynebacterium humireducens (Corynebacterium humireducens).
Embodiment 4: Corynebacterium humireducens of the present invention is to utilize lactic acid reduction AQDS test at 9 o'clock at pH
Liquid culture based formulas: contain ironic citrate 2.25g in every liter of deionized water, NaHCO
32.5g, NH
4Cl 0.25g, NaH
2PO
42H
2O 0.678g, KCl 0.1g, each 10.0mL of vitamin solution and trace element solution (vitamin solution and trace element solution composition are with embodiment 1), regulate pH with 0.5mol/L NaOH and be respectively 9, in 121 ℃ of sterilizations 20 minutes, the lactic acid solution of sterilization is added in the sterilization back, mixes, and the lactic acid final concentration is 1.0g/L.Be seeded to the LB liquid nutrient medium with inoculating needle picking lawn 2 rings from the inclined-plane of preserving Corynebacterium humireducens (Corynebacterium humireducens), in 30 ℃, 180 rev/mins of shaking table activation thalline 9h make bacterial number reach 10
8(CFU/mL) order of magnitude.Inoculum size inoculation with 10% activates bacterium liquid in liquid nutrient medium, anaerobism workstation (N
2/ CO
2=80/20) following 30 ℃ leave standstill cultivation; The contrast that does not add bacterium is set simultaneously, the colour-change situation of observing nutrient solution.Got the 4mL nutrient solution every 1 day, adopt ultraviolet-visible spectrophotometer to measure and go back ortho states AQDS (AH at the 450nm place
2DS) content is with AH
2DS concentration is index, and the checking bacterial strain is electron donor reduction AQDS ability with lactic acid.The results are shown in shown in the accompanying drawing 3, ordinate zou is AH in the accompanying drawing 3
2DS concentration (unit, mmol/L), X-coordinate is time (unit, sky), curve from top to bottom is that acetate, lactic acid are the experimental result curve of electron donor and contrast successively.From accompanying drawing 3 results as can be known, the AH that generates during for electron donor with glucose
2DS concentration constantly rises, and illustrates that C.humireducens CGMCC 2452 is to utilize lactic acid reduction AQDS at 9 o'clock at system pH.
Embodiment 5: Corynebacterium humireducens of the present invention is to utilize acetate reduction AQDS test at 9 o'clock at pH
Experimental technique is with embodiment 4, and different is that electron donor is an acetate, measures AH at set intervals
2DS concentration the results are shown in accompanying drawing 3.From accompanying drawing 3 as can be known, the AH that generates during for electron donor with acetate
2DS concentration constantly rises, and illustrates that C.humireducens CGMCC2452 is to utilize acetate reduction AQDS at 9 o'clock at system pH.
Embodiment 6: Corynebacterium humireducens of the present invention is that to utilize glucose at 9 o'clock be the active checking of electrogenesis of fuel at pH
The same patent of invention of experimental technique and step " application and the electricity-generating method thereof of enteroaerogen aspect electricity generation by microorganism " (Zhou Shungui, etc. number of patent application 200810029222.0) embodiment 1.Different is that anolyte pH value is 9, and inoculum is a Corynebacterium humireducens bacteria suspension of the present invention, and glucose concn is 1.0g/L in the anolyte, and anode and cathode connects by 1000 ohm of extrernal resistances.According to the output voltage data, the volume calculated power density the results are shown in shown in the accompanying drawing 4, and ordinate zou is power density (unit, mW/m in the accompanying drawing 4
3), X-coordinate is time (unit, hour), curve from top to bottom is that lactic acid, glucose are the experimental result curve of fuel and contrast successively.From accompanying drawing 4 as can be known anolyte pH value be 9, when glucose concn is 1.0g/L, its maximum volume power density is density 112.49mW/m
3, its summation watt rating of MFC that does not connect bacterium then is zero, illustrates that Corynebacterium humireducens of the present invention can utilize the glucose electrogenesis under alkaline condition.
Embodiment 7: Corynebacterium humireducens pH of the present invention is that to utilize lactic acid at 9 o'clock be the active checking of electrogenesis of fuel
Experimental technique and step are with embodiment 6, and different is that fuel is the lactic acid of 1g/L in the anolyte, is contrast with non-refueling MFC in the anolyte, and according to the output voltage data, the volume calculated power density the results are shown in shown in the accompanying drawing 4.From accompanying drawing 4 as can be known, Corynebacterium humireducens utilizes lactic acid to be 319.65mW/m for the maximum volume power density of fuel electrogenesis
3, illustrate that Corynebacterium humireducens can utilize the lactic acid electrogenesis under alkaline condition.
Claims (6)
1. a Corynebacterium humireducens (Corynebacterium humireducens) is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 14th, 2008, and preserving number is CGMCC 2452.
2. the separation method of the described Corynebacterium humireducens of claim 1 is characterized in that may further comprise the steps:
(1) to be inoculated in the pH value be in 10 the liquid separation culture medium to the anolyte of getting the sludge microbe fuel cell;
(2) seal after drum fills high-purity mixed gas in the liquid medium of the anolyte of seed sludge microbiological fuel cell, anaerobism leaves standstill cultivation;
(3) when the nutrient solution color from colourless when becoming yellow with 10% inoculum size enrichment culture;
(4) the nutrient solution anaerobism after the enrichment culture is cultivated, picking list bacterium colony carries out isolation and purification.
3. the application of the described Corynebacterium humireducens of claim 1 is characterized in that being applied to the biology in situ reparation of organic pollutant.
4. application according to claim 3 is characterized in that using described Corynebacterium humireducens and utilize lactic acid, acetate, formic acid, propionic acid, ethanol or sucrose reduction organic pollutant under alkaline environment.
5. the application of the described Corynebacterium humireducens of claim 1 is characterized in that being applied to the disposal of resources of microbiological fuel cell generating or high density organic waste.
6. application according to claim 5 is characterized in that using described Corynebacterium humireducens utilizes microbiological fuel cell under alkaline condition lactic acid, glucose, sucrose, starch, Succinic Acid, maltose or the generating of wood sugar fuel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101692143A CN101892180B (en) | 2010-04-30 | 2010-04-30 | Corynebacterium humireducens and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101692143A CN101892180B (en) | 2010-04-30 | 2010-04-30 | Corynebacterium humireducens and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101892180A true CN101892180A (en) | 2010-11-24 |
| CN101892180B CN101892180B (en) | 2012-07-04 |
Family
ID=43101528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101692143A Expired - Fee Related CN101892180B (en) | 2010-04-30 | 2010-04-30 | Corynebacterium humireducens and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101892180B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337236A (en) * | 2011-09-01 | 2012-02-01 | 广东省生态环境与土壤研究所 | Alkaline Bacilluspseudofirmus MC02 and application thereof |
| CN102368559A (en) * | 2011-11-01 | 2012-03-07 | 浙江大学 | Alkaline microbial fuel cell |
| CN102443400A (en) * | 2011-10-22 | 2012-05-09 | 广东省生态环境与土壤研究所 | Ternary composite organic pollution repairing agent containing iron oxide, humus and reducing bacteria thereof, and preparation method of repairing agent |
| CN102618463A (en) * | 2012-03-22 | 2012-08-01 | 中国热带农业科学院环境与植物保护研究所 | Kocuria rosea with humus reduction activity |
| CN102634467A (en) * | 2012-03-09 | 2012-08-15 | 广东省生态环境与土壤研究所 | Iron reducing bacteria and application thereof |
| EP2940143B1 (en) * | 2014-04-30 | 2020-02-05 | Evonik Operations GmbH | Method for the production of l-amino acids using an alkaliphilic bacterium |
| CN111733112A (en) * | 2020-07-22 | 2020-10-02 | 广东省微生物研究所（广东省微生物分析检测中心） | Jianjun and application thereof in biological power generation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5275940A (en) * | 1990-08-30 | 1994-01-04 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing L-tryptophan by culturing a Corynebacterium glutamicum mutant |
| CN101307297A (en) * | 2007-12-21 | 2008-11-19 | 浙江大学 | Separating, purifying and identifying method with anaerobic humus respiration capability reducing bacterium |
-
2010
- 2010-04-30 CN CN2010101692143A patent/CN101892180B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5275940A (en) * | 1990-08-30 | 1994-01-04 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing L-tryptophan by culturing a Corynebacterium glutamicum mutant |
| CN101307297A (en) * | 2007-12-21 | 2008-11-19 | 浙江大学 | Separating, purifying and identifying method with anaerobic humus respiration capability reducing bacterium |
Non-Patent Citations (1)
| Title |
|---|
| 《微生物学报》 20060804 许志诚，等 厌氧条件下希瓦氏菌腐殖质还原对偶氮还原的影响 591－597 1－6 第46卷, 第4期 2 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337236A (en) * | 2011-09-01 | 2012-02-01 | 广东省生态环境与土壤研究所 | Alkaline Bacilluspseudofirmus MC02 and application thereof |
| CN102443400A (en) * | 2011-10-22 | 2012-05-09 | 广东省生态环境与土壤研究所 | Ternary composite organic pollution repairing agent containing iron oxide, humus and reducing bacteria thereof, and preparation method of repairing agent |
| CN102443400B (en) * | 2011-10-22 | 2014-01-08 | 广东省生态环境与土壤研究所 | Ternary composite organic pollution repairing agent containing iron oxide, humus and reducing bacteria thereof, and preparation method of repairing agent |
| CN102368559A (en) * | 2011-11-01 | 2012-03-07 | 浙江大学 | Alkaline microbial fuel cell |
| CN102368559B (en) * | 2011-11-01 | 2013-10-30 | 浙江大学 | Alkaline microbial fuel cell |
| CN102634467A (en) * | 2012-03-09 | 2012-08-15 | 广东省生态环境与土壤研究所 | Iron reducing bacteria and application thereof |
| CN102634467B (en) * | 2012-03-09 | 2013-03-06 | 广东省生态环境与土壤研究所 | Iron reducing bacteria and application thereof |
| CN102618463A (en) * | 2012-03-22 | 2012-08-01 | 中国热带农业科学院环境与植物保护研究所 | Kocuria rosea with humus reduction activity |
| CN102618463B (en) * | 2012-03-22 | 2013-06-05 | 中国热带农业科学院环境与植物保护研究所 | Kocuria rosea with humus reduction activity |
| EP2940143B1 (en) * | 2014-04-30 | 2020-02-05 | Evonik Operations GmbH | Method for the production of l-amino acids using an alkaliphilic bacterium |
| CN111733112A (en) * | 2020-07-22 | 2020-10-02 | 广东省微生物研究所（广东省微生物分析检测中心） | Jianjun and application thereof in biological power generation |
| CN111733112B (en) * | 2020-07-22 | 2020-11-10 | 广东省微生物研究所（广东省微生物分析检测中心） | Jianjun and application thereof in biological power generation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101892180B (en) | 2012-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101892180B (en) | Corynebacterium humireducens and application thereof | |
| CN105462874B (en) | A kind of clostridium butyricum and its application in hydrogen production through anaerobic fermentation | |
| CN102337236B (en) | Alkaline Bacilluspseudofirmus MC02 and application thereof | |
| CN110511882A (en) | A kind of salt tolerant and the bacterial strain with electricity production characteristic and its application in microbiological fuel cell | |
| CN102399722B (en) | Bacillus cereus with electrogenesis characteristic and application thereof in microbiological fuel cell | |
| CN103898005B (en) | Electro-chemical activity antibacterial and screening technique thereof | |
| CN107217023B (en) | Multifunctional clostridium bifermentans and application thereof | |
| US8003344B2 (en) | Microbial hydrogen-producing process and system thereof | |
| Borah et al. | Construction of double chambered microbial fuel cell (MFC) using household materials and Bacillus megaterium isolate from tea garden soil | |
| CN103509735A (en) | Tolumonas osonensis bacterial strain with electrogenesis characteristic, and applications thereof in microbial fuel cells | |
| CN101880638A (en) | Shewanella and application thereof in microbiological fuel cell | |
| CN101096645A (en) | Gas production enterobacteria and uses thereof | |
| CN108285881B (en) | Mycobacterium with synchronous electricity generation and denitrification activity and application thereof | |
| CN102978274A (en) | Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent | |
| CN111733112B (en) | Jianjun and application thereof in biological power generation | |
| CN103952334B (en) | A kind of strain HD 385 and method of microorganism fermenting and producing L-erythrulose | |
| CN101712939B (en) | Lactobacillus mucosae capable of resisting high-concentration butanol | |
| CN108285880B (en) | Bacillus with electrogenesis characteristic and denitrification activity and application thereof | |
| CN103337651B (en) | A kind of Biological-agent fuel cell | |
| CN114686404B (en) | Strain with iron reduction capability and power generation capability and application thereof | |
| Indriyani et al. | Bioelectrochemical assessment of a novel electrogenic Bacillus altitudinis AC11. 2 for electricity generation in microbial fuel cell (MFC) system | |
| CN111100822B (en) | Klebsiella pneumoniae-like strain and application thereof in preparation of microbial fuel cell | |
| CN103337652B (en) | A kind of fuel cell | |
| CN101586093A (en) | Enterobacter aerogenes and application thereof | |
| CN110760469B (en) | Desulfurization vibrio and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Tianhe District Tianyuan road Guangzhou City, Guangdong province 510650 No. 808 Patentee after: GUANGDONG INSTITUTE OF ECO-ENVIRONMENTAL SCIENCE & TECHNOLOGY Address before: Guangzhou City, Guangdong province 510650 Tianyuan Road No. 808 Patentee before: GUANGDONG INSTITUTE OF ECO-ENVIRONMENT AND SOIL SCIENCES |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120704 |