CN103337651B - A kind of Biological-agent fuel cell - Google Patents
A kind of Biological-agent fuel cell Download PDFInfo
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- CN103337651B CN103337651B CN201310241350.2A CN201310241350A CN103337651B CN 103337651 B CN103337651 B CN 103337651B CN 201310241350 A CN201310241350 A CN 201310241350A CN 103337651 B CN103337651 B CN 103337651B
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- bacillus
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- pseudomonas aeruginosa
- rhodopseudomonas palustris
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- 239000000446 fuel Substances 0.000 title claims abstract description 43
- 239000003124 biologic agent Substances 0.000 title claims abstract description 12
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 16
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 15
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 15
- 230000000813 microbial effect Effects 0.000 claims abstract description 15
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 11
- 241000205101 Sulfolobus Species 0.000 claims abstract description 10
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000005864 Sulphur Substances 0.000 claims abstract description 10
- 235000019260 propionic acid Nutrition 0.000 claims abstract description 10
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims abstract description 10
- 230000009467 reduction Effects 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 5
- 241000605800 Desulfobulbus propionicus Species 0.000 claims description 4
- 238000005341 cation exchange Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 241001494297 Geobacter sulfurreducens Species 0.000 claims description 3
- 244000005700 microbiome Species 0.000 abstract description 7
- 230000007547 defect Effects 0.000 abstract description 4
- 239000000758 substrate Substances 0.000 abstract description 3
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 8
- 230000002906 microbiologic effect Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000010842 industrial wastewater Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000000306 component Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- -1 potassium ferricyanide Chemical compound 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241001103617 Pseudomonas aeruginosa ATCC 15442 Species 0.000 description 1
- 241000863430 Shewanella Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000010405 anode material Substances 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000010406 cathode material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000010248 power generation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention discloses a kind of Biological-agent fuel cell, comprise Microbial fuel, this Microbial fuel is by bacillus pumilus, and bacillus licheniformis, Rhodopseudomonas palustris, pseudomonas aeruginosa, sulphur reduction ground bacillus, propionic acid sulfolobus mixes.Microbial fuel of the present invention is by selecting suitable electrogenesis microbe, greatly improve the efficiency of fuel cell generation of microbe, and between multiple-microorganism, good symbiosis is worked in coordination with, and overcomes prior art single electrogenesis microorganism efficiency low, the defects such as substrate is single, possess good application prospect.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of Biological-agent fuel cell.
Background technology
Biological fuel cell (biofuel cell) is that one utilizes enzyme or microorganism cultures as catalyst, is the Blast Furnace Top Gas Recovery Turbine Unit (TRT) of electric energy by the chemical energy of fuel.The many of current research is microbiological fuel cell, its basic functional principle is: under anode chamber's anaerobic environment, organic substance decomposes and discharges electronics and proton under microbial action, electronics relies on suitable electron transfer mediator effectively to transmit between biological components and anode, and be delivered to negative electrode formation electric current by external circuit, and proton is delivered to negative electrode by proton exchange membrane, oxidant (being generally oxygen) obtains electronics at negative electrode and is reduced and is combined into water with proton.
Compared with the technology of existing other utilization organic substance production capacity, microbiological fuel cell has operation advantage above and functionally: first, substrate is converted into electric energy by it, ensure that to have high energy conversion efficiency; Secondly, be different from existing all biological energy source process, microbiological fuel cell can effective operation under normal temperature environment condition; 3rd, microbiological fuel cell does not need to carry out exhaust-gas treatment, because the key component of waste gas that it produces is carbon dioxide, avoids environmental pollution, is conducive to energy-saving and emission-reduction; 4th, microbiological fuel cell does not need to input comparatively macro-energy, if because single-chamber microbial fuel cell only need ventilate just can be passive supplementary cathode gas; 5th, lacking some areas of power infrastructures, microbiological fuel cell has the potentiality of extensive use, have also been enlarged for meeting our diversity to the fuel of energy demand simultaneously.
Along with the development of process of industrialization, environmental pollution and energy scarcity are two challenging greatly of facing of the world today.On the one hand, fossil fuel exhaustion is increasingly serious with global warming problem, forces countries in the world to pay much attention to the research and development of regenerative resource.Build and stablize various energy resource system, become the important component part of national strategy safety and sustainable development.On the other hand, the disposal of the organic waste that quantity is day by day huge, has become serious social concern.Microbiological fuel cell, has broad application prospects in waste water treatment and new energy development field.Although found a lot of electrogenesis microbe at present, as Shewanella, bacillus, klebsiella etc., these bacterial classifications all can only electrogenesis in neutral conditions.In theory, alkali condition can suppress the generation of methane thus be conducive to electric energy to export, and alkaline waste water is the important component part of industrial wastewater.How the electron transmission that organic metabolism produces is an important directions of MFC research by electrogenesis microbe to electrode all the time, therefore, the microorganism electricity generation mechanism studied under alkali condition the electric energy of MFC is exported and the biological treatment of alkaline waste water all significant.
At present, be the technical problem that researcher makes earnest efforts solving for the generating efficiency how improving microbiological fuel cell, wherein, the electrogenesis microbe as MFC anode catalyst is core.The defects such as at present, the electrogenesis microbe found has tens kinds, and they are anaerobic bacteria or facultative anaerobe mostly, all there is efficiency of fuel cell generation low, and the ability of refuse of disposing of sewage is more weak; The performance how improving electrogenesis microbe is prior art technical problem urgently to be resolved hurrily.
Summary of the invention
In order to overcome the defect of prior art, the object of this invention is to provide a kind of Biological-agent fuel cell utilizing microbe production capacity; Present invention also offers a kind of complex microorganism fuel.
The electricity generation ability of Biological-agent fuel cell of the present invention is strong, and make use of trade effluent, turns waste into wealth, kills two birds with one stone.
The present invention adopts following technical scheme to realize:
A kind of Biological-agent fuel cell, comprises negative electrode, catholyte, anode, anolyte, Microbial fuel, cation-exchange membrane, wire and resistance, gets up to form a loop by negative electrode, anode and resistant series by wire; Catholyte and anolyte separate by cation-exchange membrane, and anolyte passes into high pure nitrogen and ensures to be in anaerobic state.
Described cathode material and anode material are the material of this area routine, can be graphite or stainless steel; Described wire is conductive metal wire, as copper cash etc.; Described resistance is adjustable resistor.
Anolyte is: NH
4cl 1.3%, NaHCO
30.3%, KH
2pO
40.2%, glucose or sucrose 2%, all the other are water (weight fraction); Or chemical fertilizer factory's industrial wastewater.
Catholyte is: the potassium ferricyanide solution (weight fraction) of concentration 8%.
The invention also discloses a kind of Biological-agent fuel cell, it is prepared from by the raw material bacterium of following weight percents:
Bacillus pumilus 20%, bacillus licheniformis 20%, Rhodopseudomonas palustris 20%, pseudomonas aeruginosa 15%, sulphur reduction ground bacillus 15%, propionic acid sulfolobus 10%.
Described bacillus pumilus (Bacillus pumilus) specifically can be CGMCC No.5056 (for example, see CN201110347526.3);
Described bacillus licheniformis (Bacillus licheniformis) can be preferably CCTCC NO.M206082 (for example, see CN101037659A);
Described Rhodopseudomonas palustris (Rhodopseudomonas palustris) is preferably ATCC 17001 (for example, see document K Alef, D Kleiner-Archives of Microbiology, 1982)
Described pseudomonas aeruginosa (Pseudomonas aeruginosa) is preferably pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC 15442 (for example, see document Adaptation of Pseudomonasaeruginosa ATCC 15442 to didecyldimethylammonium bromide induces changes in membranefatty acid composition and in resistance of cells, Journal of Applied Microbiology, 2001);
Described sulphur reduction ground bacillus (Geobacter sulfurreducens) preferably ATCC 51573 (for example, see RCord-Ruwisch, et al, Applied and environmental, 1998);
Described propionic acid sulfolobus (Desulfobulbus propionicus) is ATCC 33891 (for example, see EA Greene, etal, Environmental, 2003) preferably.
Bacterial classification of the present invention all can from China Committee for Culture Collection of Microorganisms's common micro-organisms center, China typical culture collection center and American Type Culture collection warehousing (ATCC) buy and obtain.
Wherein each strain fermentating liquid preparation process is:
The independent expansion of various bacterial classification is cultivated:
First bacillus pumilus or bacillus licheniformis test tube kind are seeded on beef-protein medium, 28-30 DEG C, make primary inclined plane to cultivate, then be inoculated in triangular flask and do the cultivation of vibration secondary liquid, then proceed to liquid fermentation tank and do three grades of Liquid Culture, reach 1.0 × 10 to the viable count in zymotic fluid
8individual/ml;
By Rhodopseudomonas palustris or pseudomonas aeruginosa first on culture medium, 28-30 DEG C, makes primary inclined plane and cultivates, and then secondary seed is cultivated, Liquid mixed fermentation is cultured to viable count and reaches 1.0 × 10
8individual/ml, described medium component is: NH
4cl1.0g, CH
3cOONa 3.5g, MgCl
20.1g, CaCl
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract 0.1g, water 1000ml, pH7.2;
Sulphur reduction ground bacillus or propionic acid sulfolobus are inoculated into 9K culture medium ((NH respectively
4) SO
43g/L, KCl 0.1g/L, K
2hPO
40.5g/L, Ca (NO
3)
20.01g/L, FeSO
47H
2o 44.43g/L) 28-30 DEG C is cultured to viable count and reaches 1.0 × 10
8individual/nl.
The strain fermentating liquid of above-mentioned cultivation is mixed to get mixed bacteria liquid according to above-mentioned weight fraction namely can be used as fuel or make other forms such as freeze-dried powder.
The acquisition pattern of above-mentioned strain fermentating liquid is only optimal way, and in above-mentioned steps, bacterial classification expands the method for cultivating is not unique, and those skilled in the art can select suitable culture medium according to general knowledge and expand cultural method, make viable count reach 10
8individual/gram.
Compared with prior art, tool of the present invention has the following advantages:
(1) the present invention is through theory analysis for many years and actual tests, select suitable electrogenesis microbe, cultivated by multiple-microorganism symbiosis, greatly can improve the efficiency of fuel cell generation of microbe, and good symbiosis is worked in coordination with between multiple-microorganism, overcome the single electrogenesis microorganism efficiency of prior art low, the defects such as substrate is single;
(2) with the addition of bacillus licheniformis and bacillus pumilus, above-mentioned two kinds of bacterial strains all have takes oxygen function by force, effectively can remove the remnant oxygen in anolyte, and can promote the propagation of other facultative anaerobes;
(3) not only can utilize the organic substances such as glucose, sucrose, soluble starch, the various materials such as industrial wastewater can also be utilized to carry out electrogenesis, oxidation operation is thorough, and COD clearance is high;
(4) Biological-agent fuel cell structure of the present invention is simple, does not need too many maintenance, construction and operating cost low; Electric energy can be produced while removal industrial pollution, kill two birds with one stone.
Embodiment
Below employing specific embodiment is further explained the present invention, but should not regards the restriction to initiative spirit of the present invention as.
Embodiment 1
A kind of Biological-agent fuel cell, comprise negative electrode (20cm × 20em carbon felt), catholyte, anode (20cm × 20cm carbon felt), anolyte, Microbial fuel, cation-exchange membrane, copper wire wire and resistance (1000 ohm), negative electrode, anode and resistant series to be got up formation loop by wire; Anolyte is: NH
4cl 1.3%, NaHCO
30.3%, KH
2pO
40.2%, glucose 2%, all the other are water (weight fraction); Anolyte passes into high pure nitrogen and ensures to be in anaerobic state.Catholyte is: the potassium ferricyanide solution (weight fraction) of concentration 8%;
Mentioned microorganism fuel, it is prepared from by the raw material bacterium of following weight percents: bacillus pumilus 20%, bacillus licheniformis 20%, Rhodopseudomonas palustris 20%, pseudomonas aeruginosa 15%, sulphur reduction ground bacillus 15%, propionic acid sulfolobus 10%.Microbial fuel is mixed bacteria liquid, and inoculum concentration is 5% of anolyte, generates electricity after 24 hours, and the voltage at resistance two ends is 906mv, then Real-Time Monitoring voltage, by supplementary anolyte water ME for maintenance.
Embodiment 2
Anolyte is Linshu chemical fertilizer factory industrial wastewater, Microbial fuel selects specific microbial strains, bacillus pumilus (Bacillus pumilus) CGMCC No.5056, bacillus licheniformis (Bacillus licheniformis) CCTCCNO.M206082, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ATCC 17001, pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC 15442, sulphur reduction ground bacillus (Geobactor sulfurreducens) ATCC51573, propionic acid sulfolobus (Desulfobulbus propionicus) ATCC33891, all the other are the same.The composition transfer of anolyte and the anolyte of generating after 36 hours is in table 1:
Table 1
| Anolyte (mg/L) before generating | Anolyte (mg/L) after 36 hours | Clearance | |
| COD | 3698 | 443 | 88.0％ |
| Ammonia nitrogen | 767 | 81 | 89.4％ |
| Volatile phenol | 391 | 32 | 91.8％ |
Meanwhile, the voltage detecting resistance two ends is 763mv; Visible, Biological-agent fuel cell of the present invention not only power generation performance is good, but also can process the industrial wastewater of high density pollution, also can as the effective sewage-treating agent of one.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (3)
1. a Biological-agent fuel cell, comprise negative electrode, catholyte, anode, anolyte, Microbial fuel, cation-exchange membrane, wire and resistance, it is characterized in that, described Microbial fuel is prepared from by the raw material bacterium of following weight percents: bacillus pumilus 20%, bacillus licheniformis 20%, Rhodopseudomonas palustris 20%, pseudomonas aeruginosa 15%, sulphur reduction ground bacillus 15%, propionic acid sulfolobus 10%, described bacillus pumilus (Bacillus pumilus) is CGMCC No 5056, described bacillus licheniformis (Bacillus licheniformis) is CCTCC NO M206082, described Rhodopseudomonas palustris (Rhodopseudomonas palustris) is ATCC 17001, described pseudomonas aeruginosa (Pseudomonas aeruginosa) is ATCC 15442, described sulphur reduction ground bacillus (Geobacter sulfurreducens) is ATCC 51573, described propionic acid sulfolobus (Desulfobulbus propionicus) is ATCC 33891.
2., for a Microbial fuel for fuel cell, be prepared from by the raw material bacterium of following weight percents: bacillus pumilus 20%, bacillus licheniformis 20%, Rhodopseudomonas palustris 20%, pseudomonas aeruginosa 15%, sulphur reduction ground bacillus 15%, propionic acid sulfolobus 10%, described bacillus pumilus (Bacillus pumilus) is CGMCC No 5056, described bacillus licheniformis (Bacillus licheniformis) is CCTCC NO M206082, described Rhodopseudomonas palustris (Rhodopseudomonas palustris) is ATCC 17001, described pseudomonas aeruginosa (Pseudomonas aeruginosa) is ATCC 15442, described sulphur reduction ground bacillus (Geobacter sulfurreducens) is ATCC 51573, described propionic acid sulfolobus (Desulfobulbus propionicus) is ATCC 33891.
3. Microbial fuel according to claim 2 application in a fuel cell.
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| CN102219299A (en) * | 2011-04-01 | 2011-10-19 | 中国科学院成都生物研究所 | Wastewater anaerobic oxidation and negative oxygen ion coupled generation device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102906246A (en) * | 2010-02-23 | 2013-01-30 | 贝克蒂瑞罗博蒂克斯有限责任公司 | Improved microbial fuel cell |
| CN102219299A (en) * | 2011-04-01 | 2011-10-19 | 中国科学院成都生物研究所 | Wastewater anaerobic oxidation and negative oxygen ion coupled generation device and method |
| CN102381822A (en) * | 2011-07-18 | 2012-03-21 | 刘建伦 | Multi-strain compound microbial high-temperature rapid-digestion sludge harmless treatment method |
| CN102399723A (en) * | 2011-11-07 | 2012-04-04 | 南京大学 | Bacillus with electrogenesis characteristic and application thereof to microbiological fuel cell |
Non-Patent Citations (1)
| Title |
|---|
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