CN102634467A - Iron reducing bacteria and application thereof - Google Patents
Iron reducing bacteria and application thereof Download PDFInfo
- Publication number
- CN102634467A CN102634467A CN2012100607299A CN201210060729A CN102634467A CN 102634467 A CN102634467 A CN 102634467A CN 2012100607299 A CN2012100607299 A CN 2012100607299A CN 201210060729 A CN201210060729 A CN 201210060729A CN 102634467 A CN102634467 A CN 102634467A
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- sgz
- soil
- iron
- nov
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 12
- 241000894006 Bacteria Species 0.000 title claims description 11
- 239000002689 soil Substances 0.000 claims description 28
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 241000998651 Fontibacter ferrireducens Species 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 39
- 239000000243 solution Substances 0.000 description 14
- 241000703287 Fontibacter Species 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 235000013343 vitamin Nutrition 0.000 description 6
- 229940088594 vitamin Drugs 0.000 description 6
- 239000011782 vitamin Substances 0.000 description 6
- 229930003231 vitamin Natural products 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 150000003722 vitamin derivatives Chemical class 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- MSSUFHMGCXOVBZ-UHFFFAOYSA-N anthraquinone-2,6-disulfonic acid Chemical compound OS(=O)(=O)C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 MSSUFHMGCXOVBZ-UHFFFAOYSA-N 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- WHBMMWSBFZVSSR-UHFFFAOYSA-N 3-hydroxybutyric acid Chemical compound CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000852468 Cyclobacteriaceae Species 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 2
- CUBCNYWQJHBXIY-UHFFFAOYSA-N benzoic acid;2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=CC=C1.OC(=O)C1=CC=CC=C1O CUBCNYWQJHBXIY-UHFFFAOYSA-N 0.000 description 2
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 229940050410 gluconate Drugs 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- LVHBHZANLOWSRM-UHFFFAOYSA-N itaconic acid Chemical compound OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000011392 neighbor-joining method Methods 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- -1 rhamnosyl Chemical compound 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- TYFQFVWCELRYAO-UHFFFAOYSA-N suberic acid Chemical compound OC(=O)CCCCCCC(O)=O TYFQFVWCELRYAO-UHFFFAOYSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- XUCIJNAGGSZNQT-JHSLDZJXSA-N (R)-amygdalin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H](C#N)C=2C=CC=CC=2)O1 XUCIJNAGGSZNQT-JHSLDZJXSA-N 0.000 description 1
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- ATWHGWYKSFRYBN-UHFFFAOYSA-N Amygdaloside Natural products O1C(=O)C2(C)CCCC(C3CC4)(C)C2C1OCC3(C1)CC4C1(O)COC1OC(CO)C(O)C(O)C1O ATWHGWYKSFRYBN-UHFFFAOYSA-N 0.000 description 1
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 238000007900 DNA-DNA hybridization Methods 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 239000006154 MacConkey agar Substances 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical group C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229930008380 camphor Natural products 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000007792 gaseous phase Substances 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 235000013980 iron oxide Nutrition 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 235000019136 lipoic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 125000004151 quinonyl group Chemical group 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- CQRYARSYNCAZFO-UHFFFAOYSA-N salicyl alcohol Chemical compound OCC1=CC=CC=C1O CQRYARSYNCAZFO-UHFFFAOYSA-N 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229940005605 valeric acid Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Abstract
The invention discloses a Fontibacterferrireducenss p. nov. CCTCCM 2011498 and the application of the Fontibacterferrireducenss p. nov. CCTCCM 2011498 in iron reduction. The SgZ-2 has stronger anaerobic iron reduction capacity and potential application value in the aspect of environmental modification.
Description
Technical field
The present invention relates to a strain novel bacterial and the application in environmental engineering thereof, be specifically related to strain iron reduction
FontibacterferrireducensSp. nov. bacterium novel species, and the application in the iron reduction.
Background technology
A large amount of uses of oil, agricultural chemicals and the objectionable impurities in the municipal wastes are infiltrated soil in a large number, and the organic contamination of soil has become huge environmental problem.China has a large population, and land resources is limited, and along with the progress of urbanization, the soil organic pollution problems is particularly outstanding.
The reparation of soil pollution has two kinds, and a kind of is the dystopy reparation, is about to contaminated soil and is transferred to elsewhere landfill or processing, avoids its harm humans safety.This method can be destroyed the structure of soil inevitably, and is difficult to the reparation of deep layer contaminated soil, and running cost is high, is abandoned gradually.Another kind is the original position reparation, is a kind of biologic treating technique at source of pollution on-the-spot disposal pollutent.The nineties in 20th century; The U.S. spends the more than one hundred billion dollar to encourage emerging original position soil recovery technique in the period of 10; Wherein gaseous phase of soil extracts (soil vapor extraction), biological ventilate (bio-venting) and air spray (air sparging) because of its efficient is high, cost is low; Flexible design and characteristics such as simple to operate and developed rapidly are to use the widest original position soil restorative procedure.But this venting method still can't effectively be repaired the contaminated soil that is difficult to ventilate, like deep soil and water logging soil.
Iron average content in the earth's crust is about 5.6%, and mainly the form with ferric oxide exists.In anaerobism soil, in water logging soil and deep soil, ferric iron is the highest electron acceptor(EA) of abundance.Soil ulmin is to be rich in natural organic mixture quinonyl, heterogeneous, is the main body of the soil organism, accounts for 60～80% of soil organic carbon.According to statistics, in some flooded soils and freshwater sediment, the Fe of mikrobe (III) breathes (reduction)/humus respiration and has directly caused the organic carbon degraded more than 80%, far above the summation of other anaerobic respirations.Therefore, under the anaerobic condition, can utilize soil ulmin and ferric iron as electron acceptor(EA) simultaneously, can more effectively realize the original position soil reparation better with the organic pollutant mineralising like mikrobe.
FontibacterBelong to the sphingolipid Bacteriaceae (
Cyclobacteriaceae), set up in 2010 by people such as Kampfer at first.Its paper (
Fontibacter flavusGen. nov., sp. nov., a member of the family '
Cyclobacteriaceae', isolated from a hot spring, Peter K mpfer
Et al. IJSEM September 2010 vol. 60 no. 9 2066-2070
) when delivering, this subordinate only has a kind
Fontibacter flavusSo far, find to have the iron reducing power
FontibacterBacterial strain.
Summary of the invention
The object of the present invention is to provide a strain to have the novel bacterial of iron reducing power:
FontibacterferrireducensSp. nov. SgZ-2.
Another object of the present invention is to provide the application of above-mentioned bacterial strains in the soil original position is repaired.
The applicant submitted bacterial strain to be positioned at Hubei Wuhan University Chinese typical culture collection center preservation on October 28th, 2011, and the bacterial strain that the applicant provides is received at Chinese typical culture collection center on December 31st, 2011
FontibacterferrireducensSp. nov. SgZ-2, the preserving number that the preservation center gives this culture does
CCTCC M2011498, the classification called after of suggestion
FontibacterferrireducensSgZ-2 is survived in the bacterial strain of evaluation preservation on January 10th, 2012.
Bacterial strain of the present invention
FontibacterferrireducensSp. nov. SgZ-2; Soil ulmin under anaerobic capable of using and ferric iron are electron acceptor(EA), quicken organic degraded, and therefore original position is removed the organic pollutant in the soil effectively; Realize the original position reparation of soil cheaply; Can not destroy simultaneously the structure of soil, help the utilization of land resources, aspect environment remediation, be with a wide range of applications.
Description of drawings:
Fig. 1: 5000 * transmission electron microscope photo of bacterial strain of the present invention;
Fig. 2: 3000 * transmission electron microscope photo of bacterial strain of the present invention;
Fig. 3: the 16S rRNA Phylogenetic Tree of bacterial strain of the present invention;
Fig. 4-7: bacterial strain of the present invention uses different electron donors to reduce the experimental result picture of different Fe (III).
Embodiment
The enrichment of bacterial strain SgZ-2 with separate
From 3 months sludge microbe fuel cell of continuous service, tear and get the anode microbial film and be soaked in the mineral salts medium of 20 mL sterilization, the composition of mineral salts medium (MSM) is: 0.6 g NaH
2PO
4, 0.25 g NH
4Cl, 0.1 g KCl, 0.2 g yeast extract, 10.0ml vitamin solution, 10.0ml trace element solution; And the glucose of AQDS and 5 mM that is added with 0.5 mM is respectively as electron acceptor(EA) and electron donor; PH regulator to 7.2; Wherein, vitamin solution is to contain 2.0 mg vitamin Hs, 2.0 mg folic acid, VB in every liter of deionized water
610.0 mg VITAMINs, 5.0 mg VitB1s, 5.0 mg vitamin G, 5.0 mg nicotinic acid, 5.0 mg VAs, 0.1 mg vitamins B
12, 5.0 mg para-amino benzoic acid, 5.0 mg Thioctic Acids; Trace element solution is to contain 1.5 g nitrilotriacetic acid(NTA)s, 3.0 g MgSO in every liter of deionized water
47H
2O, 0.5 g MnSO
4H
2O, 1.0 g NaCl, 0.1 g FeSO
47H
2O, 0.1 g CoCl
26H
2O, 0.1 g CaCl
2, 0.1 g ZnSO
47H
2O, 0.01 g CuSO
45H
2O, 0.01 g AlK (SO
4)
212H
2O, 0.01 g H
3BO
3, 0.01 g Na
2MoO
42H
2O;
In vaccinated enrichment medium, fill gas mixture (N
2/ CO
2=80/20) 15 minutes, air-blowing finished and covers serum cap and add aluminium lid sealing, places the anaerobism workstation, and 30 ℃ leave standstill cultivation, observe the colour-change situation of nutrient solution;
Behind the 5d, the nutrient solution color becomes when orange-yellow from colourless, nutrient solution is applied on the MSM agar (20g agar, 1 mM AQDS and 5 mM glucose) 30 ℃ of anaerobism workstation anaerobism (N
2/ CO
2=80/20) cultivates;
Screening take on a red color on the flat board for can carry out AQDS reductive bacterium colony, use TSA or TSB substratum separate, and obtain bacterial strain SgZ-2.
The morphological specificity of bacterial strain SgZ-2
1) thalli morphology characteristic
Bacterial strain SgZ-2 is a Gram-negative bacteria, does not produce gemma; Transmission electron microscope result (Fig. 1 and 2) shows the bacterial strain atrichia, and bar-shaped, wide about 0.2-0.4 μ m is about 1.5-3.0 μ m.
) the colonial morphology characteristic
Bacterium colony is orange, convexity, and the edge is rounded, the about 2-4 mm of diameter.
The physiological and biochemical property of bacterial strain SgZ-2
According to the physiological and biochemical property of " uncle outstanding Bacteria Identification identification handbook " the 9th edition described standard program test strain, and utilize API ID32GN, 20E, ZYM experiment bar detect other physico-chemical property and with
Fontibacter flavusCCM 7650
T Compare, bacterial strain SgZ-2 is non-fermented type facultative anaerobe, and the result sees the following form:
The optimum N aCl content of bacterial strain SgZ-2 growth is 1%, and growth temperature is 15～37 ℃ (optimum growth temp is 25～30 ℃), optimum growh pH 7.0.Well-grown on nutrient agar medium is not grown on MacConkey agar.
Bacterial strain SgZ-2 glucose capable of using, melibiose, sucrose, trehalose fermentation and acid, but N.F,USP MANNITOL, inositol, rhamnosyl, amygdaloside and pectinose fermentation and acid can not be utilized; Can utilize glucose, saligenin, melibiose, trehalose, sorbyl alcohol, sucrose and SANMALT-S as single carbon source, cannot utilize N.F,USP MANNITOL, pectinose, propionic acid, capric acid, valeric acid, Hydrocerol A (salt), Histidine, 2-ketone gluconate, 3-hydroxybutyric acid, Whitfield's ointment, proline(Pro), rhamnosyl, N-acetylglucosamine, ribose, inositol, methylene-succinic acid, suberic acid, acetate, lactic acid salt, L-Ala, 5-ketone group-gluconate, Whitfield's ointment, glycogen or Serine as single carbon source.
The chemotaxonomy characteristic of bacterial strain SgZ-2
For confirming the classification position of invention bacterial strain, measured the fatty acid content of bacterial strain SgZ-2 cell, and with
Fontibacter flavusCCM 7650
T Compare, the result is as shown in table 2.
*Summed feature 3 comprises C
16:1 ω6
cAnd/or C
16:1 ω7
c; Summed feature 8 comprises one or more of 17:1 iso I/anteiso B, three kinds of components of 17:1 anteiso B/iso I; Summed feature 9 comprises 17:1 iso
ω9c and/or 16:0 10-methyl.
The molecular classification status of bacterial strain SgZ-2
Extract total DNA of invention bacterial strain; DNA to extract is a template, and the 16S rRNA gene of amplification bacterium obtains the 16S rRNA sequence of almost complete (1429bp), checks order after pcr amplification product is reclaimed purifying, and the gene accession number of institute's calling sequence in GenBank is JQ348962.
Online comparison instrument through EzTaxon server 2.1 websites is compared, and 16S rRNA gene sequencing result shows, bacterial strain SgZ-2 with
FontibacterUnique member in the genus
Fontibacter flavusThe 16S rRNA gene order similarity of CCM7650 is the highest, is 99.4%, and in belonging to other similaritys of bacterial strain all less than 94%.
The phylogenetic tree that uses the Neighbor-joining method to make up is as shown in Figure 3.In the phylogenetic tree that the Neighbor-joining method makes up, bacterial strain SgZ-2 with
Fontibacter flavusCCM 7650
TBe in different inferior branches.
For further confirm SgZ-2 with
Fontibacter flavusCCM 7650
TBetween taxonomic relation, press Ezaki
Et al. the method that (1989) are described is carried out DNA-DNA hybridization to it.The result show bacterial strain SgZ-2 with
Fontibacter flavusCCM 7650
TDNA-DNA dependency very low (quadrature 35.7%, reciprocal cross 40%), far below standard of perfection of the same race 70%, show that bacterial strain SgZ-2 is a new bacterial classification.
Comprehensive above-mentioned morphological feature, physio-biochemical characteristics and Phylogenetic Analysis, bacterial strain of the present invention due to
FontibacterBelong to, and be defined as novel species, with its called after
Fontibacter ferrireducensSp. nov..
Bacterial strain SgZ-2 under different electron donors to the reductive action of different Fe (III)
1) adopts the liquid culture based formulas: 0.25g NH in every liter of deionized water
4Cl, 0.678g NaH
2PO
42H
2O, 0.1g KCl, 2.94g NaHCO
3, 1.59g Na
2CO
3, each 10.0 mL (vitamin solution and the same isolation medium of trace element solution composition) of vitamin solution and trace element solution; After the sterilization, the glucose that adds 10 mM in the system is as electron donor, and the four kinds of ferriferous oxides (ferrihydrite, pyrrhosiderite, lepidocrokile and rhombohedral iron ore) that add 25 mM respectively are as electron acceptor(EA);
2) be seeded to the TSB substratum from the inclined-plane picking lawn of preserving bacterial strain SgZ-1, in 30 ℃, 180 rpm shaking table activation thalline 18 hours make bacterial number reach exponential phase of growth;
3) 4 ℃, centrifugal 10 min of 6000 rpm remove supernatant, and carbonic acid buffer suspends again, repeat aforesaid operations 2 times, and the concentration of final suspension-s reaches OD
600About 1.2;
4) add 1 ml thallus suspension liquid as processing, use substratum that does not add bacterial strain SgZ-2 and the substratum that adds bacterial strain SgZ-2 but do not add glucose are set simultaneously as contrast respectively, the same terms is cultivated down;
5) every got at a distance from 5 days the 4 ml nutrient solutions that mix in 16ml 0.5mol/l HCl solution 180 rev/mins vibrated 1.5 hours; Refilter; Adopt adjacent phenanthroline method to give the Fe (II) in the filtrating colour developing; Adopt ultraviolet spectrophotometer to measure the content of Fe (II) at 510 nm places, and be contrast, verify the iron reducing power of bacterial strain with the nutrient solution that does not add bacterium.
Test-results is shown in Fig. 4～7, and bacterial strain SgZ-2 can utilize glucose to be the electron donor reducing iron oxides, and is wherein the highest to the reduction efficiency of ferrihydrite, is lepidocrokile, pyrrhosiderite and rhombohedral iron ore successively secondly.
Claims (2)
1. iron-reducing bacterium
FontibacterferrireducensSp. nov. SgZ-2 is deposited in Chinese typical culture collection center, and its preserving number does
CCTCC M2011498
2. the said iron reduction of claim 1
FontibacterferrireducensSp. the application of nov. bacterium SgZ-2 in the soil original position is repaired.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210060729 CN102634467B (en) | 2012-03-09 | 2012-03-09 | Iron reducing bacteria and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210060729 CN102634467B (en) | 2012-03-09 | 2012-03-09 | Iron reducing bacteria and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102634467A true CN102634467A (en) | 2012-08-15 |
| CN102634467B CN102634467B (en) | 2013-03-06 |
Family
ID=46619046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210060729 Expired - Fee Related CN102634467B (en) | 2012-03-09 | 2012-03-09 | Iron reducing bacteria and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102634467B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103752604A (en) * | 2014-01-21 | 2014-04-30 | 广东省生态环境与土壤研究所 | Method for repairing heavy metal contaminated soil by combination of biochar and iron-reducing bacteria agent |
| CN104458734A (en) * | 2014-12-19 | 2015-03-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN105670965A (en) * | 2016-02-25 | 2016-06-15 | 广东省生态环境与土壤研究所 | Novel strain with iron reduction capability and application thereof |
| CN108821444A (en) * | 2018-05-28 | 2018-11-16 | 天津大学 | Anaerobism phosphorus recovery method based on microorganism alienation metal reduction |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892180A (en) * | 2010-04-30 | 2010-11-24 | 广东省生态环境与土壤研究所 | Corynebacterium humireducens and application thereof |
-
2012
- 2012-03-09 CN CN 201210060729 patent/CN102634467B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892180A (en) * | 2010-04-30 | 2010-11-24 | 广东省生态环境与土壤研究所 | Corynebacterium humireducens and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 武春媛等: "腐殖质呼吸作用及其生态学意义", 《生态学报》 * |
| 王弋博等: "一株腐殖质和铁还原菌的分离和鉴定", 《兰州大学学报(自然科学版)》 * |
| 王弋博等: "韩国丛毛单胞菌CY01的铁还原和腐殖质还原", 《兰州大学学报(自然科学版)》 * |
| 许伟等: "微生物铁呼吸机制研究进展", 《生态学杂志》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103752604A (en) * | 2014-01-21 | 2014-04-30 | 广东省生态环境与土壤研究所 | Method for repairing heavy metal contaminated soil by combination of biochar and iron-reducing bacteria agent |
| CN103752604B (en) * | 2014-01-21 | 2015-12-02 | 广东省生态环境与土壤研究所 | A kind of method of charcoal and Fe3+ reduction microbial inoculum renovation of heavy metal polluted soil with combined |
| CN104458734A (en) * | 2014-12-19 | 2015-03-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN104458734B (en) * | 2014-12-19 | 2017-01-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN105670965A (en) * | 2016-02-25 | 2016-06-15 | 广东省生态环境与土壤研究所 | Novel strain with iron reduction capability and application thereof |
| CN105670965B (en) * | 2016-02-25 | 2020-02-21 | 广东省生态环境技术研究所 | Strain with iron reduction capacity and application thereof |
| CN108821444A (en) * | 2018-05-28 | 2018-11-16 | 天津大学 | Anaerobism phosphorus recovery method based on microorganism alienation metal reduction |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102634467B (en) | 2013-03-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Antony et al. | Diversity and physiology of culturable bacteria associated with a coastal Antarctic ice core | |
| CN106939293B (en) | Achromobacter xylosoxidans GYP4 and application thereof in degradation of brominated flame retardant | |
| CN102277312A (en) | Strain for degrading polycyclic aromatic hydrocarbons at low temperature and application in bioremediation of groundwater in petroleum hydrocarbon polluted places thereof | |
| CN102242071B (en) | Application of Pichia guilliermondii 510-6jm in treatment of petroleum hydrocarbon contaminations | |
| CN102634467B (en) | Iron reducing bacteria and application thereof | |
| CN102296037A (en) | Pseudomonas aeruginosa and separation method and application thereof | |
| CN108300674A (en) | A kind of oil degradation bacteria and its preparation method and the application in degrading crude oil | |
| JP2023544961A (en) | Kosakonia oryzae HN05 and its use | |
| CN101935631B (en) | Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil | |
| CN102634466B (en) | Thauera humireducens and application thereof and microbiological preparation | |
| CN105820963B (en) | Phanerochaete chrysosporium and its application | |
| CN105219674A (en) | A kind of hydrocarbon degradation bacteria and application thereof | |
| Gofar | Characterization of petroleum hydrocarbon decomposing fungi isolated from mangrove rhizosphere | |
| CN107841477A (en) | Application of one plant of arsenic oxidizing bacteria in rice trivalent arsenic pollution is reduced | |
| CN104877934A (en) | Salt-tolerant polycyclic aromatic hydrocarbon degradation bacterium strain Tzymx and application thereof in marine pollution environment | |
| CN106947719A (en) | A kind of burkholderia GYP1 and its application in degraded brominated flame-retardant | |
| CN102260641B (en) | High-efficiency organic pollutant carbendazim degrading bacteria and use thereof | |
| CN103952342A (en) | Pseudomonas geniculata and application thereof | |
| CN104745515B (en) | A kind of acinetobacter calcoaceticus of degrading polycyclic aromatic hydrocarbons and its application | |
| CN103382446A (en) | Rhizobium capable of degrading polychlorinated biphenyl and application thereof | |
| CN102864086B (en) | Strain capable of degrading polycyclic aromatic hydrocarbons (PAHs) and application thereof in soil remediation | |
| CN113583899B (en) | Sphingosine strain JT-M9-H as polycyclic aromatic hydrocarbon degrading strain | |
| CN105820962B (en) | Branch acremonium bacterium and its application | |
| CN105400718B (en) | One plant of nitrate relies on ferrous oxidation bacterial strain and its application | |
| CN114107101A (en) | Cadmium and chromium-fixed cupronickel bacterium and application thereof in co-remediation of cadmium and chromium composite polluted soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 510650 No. 808 Tianyuan Road, Tianhe District, Guangzhou, Guangdong Patentee after: GUANGDONG INSTITUTE OF ECO-ENVIRONMENTAL SCIENCE & TECHNOLOGY Address before: 510650 808 Tianyuan Road, Guangzhou, Guangdong Patentee before: GUANGDONG INSTITUTE OF ECO-ENVIRONMENT AND SOIL SCIENCES |
|
| CP03 | Change of name, title or address | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Tianhe District Tianyuan road Guangzhou City, Guangdong province 510650 No. 808 Patentee after: Institute of ecological environment and soil, Guangdong Academy of Sciences Address before: Tianhe District Tianyuan road Guangzhou City, Guangdong province 510650 No. 808 Patentee before: GUANGDONG INSTITUTE OF ECO-ENVIRONMENTAL SCIENCE & TECHNOLOGY |
|
| CP01 | Change in the name or title of a patent holder | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130306 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |