CN101880704A - Method for preparing peptide albumen by utilizing fermentation of microalgae grease bacterial dregs and bacterial mud and application - Google Patents
Method for preparing peptide albumen by utilizing fermentation of microalgae grease bacterial dregs and bacterial mud and application Download PDFInfo
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- CN101880704A CN101880704A CN2009100619148A CN200910061914A CN101880704A CN 101880704 A CN101880704 A CN 101880704A CN 2009100619148 A CN2009100619148 A CN 2009100619148A CN 200910061914 A CN200910061914 A CN 200910061914A CN 101880704 A CN101880704 A CN 101880704A
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Abstract
The invention provides a method for preparing peptide albumen by utilizing fermentation of microalgae grease bacterial dregs and bacterial mud. The microalgae grease bacterial dregs and bacterial mud are biologically fermented and modified through biological engineering technology and the fermentation of functional strains, the unsaturated fatty acid ingredient peculiar to the microalgae grease bacterial dregs and bacterial mud is remained, and the favorable effect of other active ingredients of the microalgae grease bacterial dregs and bacterial mud is exerted, so that the fermented products have unique activity. The microalgae grease bacterial dregs and bacterial mud which are used as the main raw materials of a substrate are added with vegetable protein raw materials which are used as auxiliary materials to perform fermentation, so the unsaturated fatty acid ingredient in the microalgae grease bacterial dregs is utilized and the side effect of residual organic solvent in the process of extracting the microalgae grease on animals is reduced to a greatest extent; an amino acid structure in the products tends to be balanced and reasonable; the protein in the products exists in the form of the peptide albumen through the enzymolysis of protease, so that the products have highest animal absorptivity and bioavailability; a plurality of anti-nutritional factors in the fermentative substrate are reduced or eliminated; and unique functions of the products are exerted in the culture of high-grade economic furry animals and the feeding of pets such as cats, dogs and the like, and in the improvement on aquatic seedling feed. At the same time, the scientific treatment and utilization of the microalgae grease bacterial dregs and bacterial mud by biotechnology are the optimal mode of an original treatment mode of the microalgae grease bacterial dregs and bacterial mud which are used for feed culture and are discharged into water, dumped and buried together with rubbish.
Description
Affiliated technical field
The present invention relates to and utilize biological fermentation that microalgae grease bacterial dregs, bacterium mud are carried out biological fermentation modification processing and utilization, belong to the fermentation engineering field.
Background technology
Microbial oil, claim Unicell Oils and Fats again, a lot of microorganisms such as bacterium, mould, yeast and algae etc. are under certain conditions, can in thalline, produce a large amount of greases, general microbial oil economic worth also can't contend with vegetables oil, research to microbial oil mainly concentrates on the grease of the high special nutrition function of production economic worth, such as unsaturated fatty acids that from microorganism, extracts such as linolic acid, arachidonic acid, linolenic acid, EPA and DHA, perhaps their ester derivative etc. is mainly used in nutritional additive in baby milk powder and the pregnant and lying-in women's milk powder, protective foods, medicine.Simultaneously, unsaturated fatty acids also is particularly particular stage, the necessary nutrient substance of extraordinary kind animal of animal, unsaturated fatty acids is as fodder additives, it can strengthen the particularly neural growth of cat and dog of fleece animal, make its alertness, active, improve its amphiblestroid growth, the enhancing immunity resistibility, especially keep coat health, beautiful and gloss.In fishes and shrimps growth and growth course, bringing into play important function, an amount of input has become an important means of increasing both production and income in aquaculture, can improve growth performance and the human body immune function of fish and shrimp, then can significantly improve survival rate and distortion ratio, promote product quality cubhood.Just contain a certain amount of unsaturated fatty acids and high-quality tropina in bacterium slag behind the microalgae grease extraction unsaturated fatty acids, the bacterium mud; In the practical application, what obtain is the mixture of microbial cells and culture medium residue, rationally science, healthy and safe to utilize limited resources are requirements of national industrial policies, human sciences's progressive important symbol.
Along with the breakthrough and the high speed development of biotechnology and correlation technique, microalgae grease (unsaturated fatty acids) industrialization is sharply expanded, and the science of microalgae grease bacterial dregs, bacterium mud, processing and utilizing does not cause enough attention all the time safely.At present, the processing mode of microalgae grease bacterial dregs, bacterium mud mainly contain enter in the water, with rubbish outwell, landfill or be used for feed without any processing and culture.Like this environment is polluted destruction, to the waste of scarce resource of height utility value is arranged, the mankind's of animal and food cycle food safety is had potential hidden danger.Utilizing biotechnology that microalgae grease bacterial dregs, bacterium mud are carried out the science processing and utilizing is the present and even following best mode.
Summary of the invention
Present technique is passed through biotechnology, adopt the fermentation of function bacterial classification, microalgae grease bacterial dregs, bacterium mud are carried out the biological fermentation modification to be handled, keep distinctive unsaturated fatty acids composition in microalgae grease bacterial dregs, the bacterium mud, bring into play the advantageous effect of other effective constituents of microalgae grease bacterial dregs, bacterium mud, thereby give the activity of fermentation back product uniqueness.With microalgae grease bacterial dregs, bacterium mud is that substrate is that to add plant protein material again be that auxiliary material ferments to main raw material, the unsaturated fatty acids composition in utilizing microalgae grease bacterial dregs, bacterium mud, at utmost reduce to extract in the microalgae grease process residual organic solvent to the negative effect of animal; Make in the product amino acid structure balance that more becomes reasonable, the enzymolysis of the protein in the product by proteolytic enzyme exists with the state of peptide protein and has splendid animal specific absorption and bioavailability; Multiple antinutritional factor in the fermentation substrate is reduced and or eliminate, make the nursing of product, and in improving the aquatic fry growing feed, bring into play its unique function pets such as high-grade fur economic animal breeds, cat and dog.
For above-mentioned purpose is achieved, the technical scheme that provides is as follows:
The present invention adopts function bacterial classification solid-fermented technique, with microalgae grease bacterial dregs, bacterium mud be substrate be main raw material to add plant protein material again be that auxiliary material ferments, inoculate, add technical matters such as water mixing, fermentation, drying, pulverizing and produce finished product.In the above-mentioned technical matters, be that to add plant protein material again be auxiliary material to the substrate main raw material with microalgae grease bacterial dregs, bacterium mud; Plant protein material is best with the dregs of beans.
In the above-mentioned technical matters, add above auxiliary material (raw material and auxiliary material are all with dry basis) by the 10%-30% of main raw material weight, and thorough mixing (artificial or machinery all can).
In the above-mentioned technical matters, described function bacterial classification is genus bacillus and yeast, and the 2-4% that presses the raw material dry weight adds bacterial classification, and wherein genus bacillus and yeast ratio are mass ratio 7-6: 4-3.
In the above-mentioned technical matters, raw material, bacterial classification mixed add entry in the process gradually, make well-mixed material moisture be controlled at 45-60%, the best is 50%.
In the above-mentioned technical matters, material ferments in the open container of cleaning or carry out in the place, and fermenting container includes but not limited to solid fermentation groove, solid-state fermentation tank and solid fermentation plate rail etc., and can or there be the ground of enclosing the ridge in the place for smooth ground.
In the above-mentioned technical matters, the thickness of mixed material should not be higher than 100cm, and preferred heights is at 60-80cm.Leavening temperature is at 30-55 ℃; The highlyest be no more than 55 ℃, when temperature is made stirring or/and the suitable attemperation of ventilation treatment measures and improve the oxygen supply situation in time more than 55 ℃.
In the above-mentioned technical matters, fermentation time is 36-60 hour (can adjust according to variation of ambient temperature), and preferred fermentation time is 48 hours.
In the above-mentioned technical matters, after fermentation was finished, drying plant adopted the non-direet-heating type drying plant of normal temperature to carry out, and temperature of charge is lower than 100 ℃.
In the above-mentioned technical matters, dry back material is pulverized, and pulverizes 80 mesh sieves, finished product water content≤10% after the pulverizing.
In the above-mentioned technical matters, finished product the growing seedlings of aquatic products, culture in 5～20% ratio of complete diet pellet and add in the feed; Finished product adds in the feed in 5～15% ratio of complete diet pellet pets such as extraordinary cultivation of fur animals and cat, dogs.
It is as follows that the present invention and existing treatment process are compared advantage:
1, the present invention utilizes biotechnology that the science of microalgae grease bacterial dregs, bacterium mud is handled and utilization; it is protection to environment; to the abundant exploitation of scarce resource of height utility value is arranged, eliminated the hidden danger that the mankind's of animal and food cycle food safety is had potential threat.2, present technique keeps distinctive unsaturated fatty acids composition in microalgae grease bacterial dregs, the bacterium mud, brings into play the advantageous effect of other effective constituents of microalgae grease bacterial dregs, bacterium mud, thereby gives the activity of fermentation back product uniqueness.3, at utmost reduce to extract in the microalgae grease process residual organic solvent to the negative effect of animal.4, make in the product amino acid structure balance that more becomes reasonable, the enzymolysis of the protein in the product by proteolytic enzyme exists with the state of peptide protein and has splendid animal specific absorption and bioavailability; Multiple antinutritional factor in the fermentation substrate is reduced and or elimination.5, the product after the fermentation is in the nursings of pets such as high-grade fur economic animal breeds, cat and dog, and brings into play its unique function in improving aquatic fry growing.
The present invention is by following specific embodiments, and described embodiment is not limited to the described content of concrete case.
Scheme 1: microalgae grease bacterial dregs 500kg, bacterium mud 1200kg (moisture 70%), dregs of beans 100kg, bacterial classification 3kg; Raw material, bacterial classification thorough mixing is even, make that material moisture is 50%, the high 70cm of heap in the solid fermentation groove.Leavening temperature is at 30-55 ℃; The highlyest be no more than 55 ℃, when temperature was made the stirring attemperation and improved the oxygen supply situation in time more than 55 ℃, fermentation time is 48 hours, after finishing, fermentation adopt the non-direet-heating type drying plant of normal temperature to carry out drying, keep temperature of charge to be lower than 100 ℃, pulverize subsequently, pulverized 80 mesh sieves, finished product water content≤10% after the pulverizing.Finished product adds in the high-grade fur economic animal feed ingredient according to 15% of complete diet pellet.
Scheme 2: microalgae grease bacterial dregs 700kg, bacterium mud 500kg (moisture 70%), dregs of beans 150kg, bacterial classification 3kg; Raw material, bacterial classification mixed add entry in the process gradually, make well-mixed material moisture 48%, the high 80cm of heap in the solid fermentation groove.Leavening temperature is at 30-55 ℃; The highlyest be no more than 55 ℃, when temperature was made stirring or ventilation attemperation and improved the oxygen supply situation in time more than 55 ℃, fermentation time is 48 hours, after finishing, fermentation adopt the non-direet-heating type drying plant of normal temperature to carry out drying, keep temperature of charge to be lower than 100 ℃, pulverize subsequently, pulverized 80 mesh sieves, finished product water content≤10% after the pulverizing.Finished product adds in the cat and dog feed for pet according to 5% of complete diet pellet.
Scheme 3: microalgae grease bacterial dregs 1000kg, bacterium mud 1000kg (moisture 70%), dregs of beans 250kg, bacterial classification 4kg; Raw material, bacterial classification mixed add entry in the process gradually, make well-mixed material moisture 55%, the high 80cm of heap in the solid fermentation groove.Leavening temperature is at 30-55 ℃; The highlyest be no more than 55 ℃, when temperature was made stirring or ventilation attemperation and improved the oxygen supply situation in time more than 55 ℃, fermentation time is 48 hours, after finishing, fermentation adopt the non-direet-heating type drying plant of normal temperature to carry out drying, keep temperature of charge to be lower than 100 ℃, pulverize subsequently, pulverized 80 mesh sieves, finished product water content≤10% after the pulverizing.Finished product ratio of 10% in complete diet pellet in the growing seedlings of aquatic products is added in the feed.
Claims (10)
1. method of utilizing microalgae grease bacterial dregs, bacterium mud fermentative preparation peptide protein, adopt function bacterial classification solid-fermented technique, with microalgae grease bacterial dregs, bacterium mud be substrate be main raw material to add plant protein material again be that auxiliary material ferments, inoculate, add technical matters such as water mixing, fermentation, drying, pulverizing and produce finished product.
2. the method that requires according to right 1, it is characterized in that with microalgae grease bacterial dregs, bacterium mud be the substrate main raw material to add plant protein material again be auxiliary material, plant protein material is best with the dregs of beans.
3. the method that requires according to right 1 is characterized in that adding above auxiliary material (raw material and auxiliary material are all with dry basis) by the 10%-30% of main raw material weight, and thorough mixing (artificial or machinery all can).
4. the method that requires according to right 1 is characterized in that described function bacterial classification is genus bacillus and yeast, and the 2-4% that presses the raw material dry weight adds bacterial classification, and wherein genus bacillus and yeast ratio are mass ratio 7-6: 4-3.
5. the method that requires according to right 1 is characterized in that raw material, bacterial classification mixed and adds entry in the process gradually, makes well-mixed material moisture be controlled at 45-60%, and the best is 50%.
6. the method that requires according to right 1, it is characterized in that material ferments in the open container of cleaning or carry out in the place, fermenting container includes but not limited to solid fermentation groove, solid-state fermentation tank and solid fermentation plate rail etc., and can or there be the ground of enclosing the ridge in the place for smooth ground.
7. the method that requires according to right 1, the thickness that it is characterized in that mixed material should not be higher than 100cm, preferred heights is at 60-80cm, leavening temperature is at 30-55 ℃, the highlyest be no more than 55 ℃, when temperature is made stirring or/and the suitable attemperation of ventilation treatment measures and improve the oxygen supply situation in time more than 55 ℃.
8. the method that requires according to right 1, it is characterized in that fermentation time is 36-60 hour (can adjust according to variation of ambient temperature), preferred fermentation time is 48 hours, after fermentation is finished, drying plant adopts the non-direet-heating type drying plant of normal temperature to carry out, and keeps temperature of charge to be lower than 100 ℃.
9. the method that requires according to right 1 is characterized in that dry back material pulverizes, and pulverizes 80 mesh sieves, finished product water content≤10% after the pulverizing.
10. the method that requires according to right 1, it is characterized in that finished product the growing seedlings of aquatic products, culture in 5～20% ratio of complete diet pellet and add in the feed, finished product adds in the feed in 5～15% ratio of complete diet pellet pets such as extraordinary cultivation of fur animals and cat, dogs.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194494A (en) * | 2013-03-22 | 2013-07-10 | 哈尔滨工业大学 | Method for producing volatile fatty acids through co-fermentation of agaricus bisporus fungus chaff and surplus sludge |
| CN103652327A (en) * | 2013-12-10 | 2014-03-26 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN103843980A (en) * | 2012-11-28 | 2014-06-11 | 武汉常辉天添生物科技有限公司 | Preparation method of mixed fermentation feed |
| CN114258981A (en) * | 2021-12-30 | 2022-04-01 | 南阳师范学院 | Heterotrophic chlorella fermented feed and preparation method thereof |
-
2009
- 2009-05-05 CN CN2009100619148A patent/CN101880704A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843980A (en) * | 2012-11-28 | 2014-06-11 | 武汉常辉天添生物科技有限公司 | Preparation method of mixed fermentation feed |
| CN103194494A (en) * | 2013-03-22 | 2013-07-10 | 哈尔滨工业大学 | Method for producing volatile fatty acids through co-fermentation of agaricus bisporus fungus chaff and surplus sludge |
| CN103194494B (en) * | 2013-03-22 | 2014-07-30 | 哈尔滨工业大学 | Method for producing volatile fatty acids through co-fermentation of agaricus bisporus fungus chaff and surplus sludge |
| CN103652327A (en) * | 2013-12-10 | 2014-03-26 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN103652327B (en) * | 2013-12-10 | 2015-04-01 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN114258981A (en) * | 2021-12-30 | 2022-04-01 | 南阳师范学院 | Heterotrophic chlorella fermented feed and preparation method thereof |
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Application publication date: 20101110 |