CN101857855B - Complex enzyme preparation and application in producing diosgenin - Google Patents
Complex enzyme preparation and application in producing diosgenin Download PDFInfo
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- CN101857855B CN101857855B CN2010102174512A CN201010217451A CN101857855B CN 101857855 B CN101857855 B CN 101857855B CN 2010102174512 A CN2010102174512 A CN 2010102174512A CN 201010217451 A CN201010217451 A CN 201010217451A CN 101857855 B CN101857855 B CN 101857855B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a complex enzyme preparation and application thereof in producing diosgenin. The complex enzyme preparation comprises an I-type complex enzyme preparation and an II-type complex enzyme preparation, wherein the I-type complex enzyme preparation comprises pectinase, cellulose, xylanase, hemicellulase and tannase; the II-type complex enzyme preparation comprises glucose and beta-glucuroide. The application of the complex enzyme preparation in producing diosgenin comprises the following steps of pretreating materials; carrying out primary hydrolysis with the I-type complex enzyme preparation; carrying out secondary hydrolysis with the II-type complex enzyme preparation; filtering the material liquid to enable solid-liquid separation; carrying out acidolysis, water-washable acid and filter-press on a solid product, drying to obtain a saponin hydrolysate; and then extracting and crystallizing according to the traditional process of saponin extraction to obtain a saponin finished product. The diosgenin produced by using the complex enzyme preparation can promote the release of active ingredients of turmeric, improve the yield, reduce the total use level of acid by more than 70% compared with the traditional process, recycle pollutants-waste sugar liquid at the same time, effectively solve the problem of water pollution in producing the diosgenin, decrease the total amount of the pollutants by more than 85% and reduce the waste discharge of diosgenin production enterprises by 40%.
Description
Technical field
The present invention relates to the extraction of plant sapogenin glycoside, specifically a kind of compound enzymic preparation and the application in diosgenin is produced.
Background technology
Rhizome of Peltate Yam claims yellow ginger again, is the herbaceous perennial vine plant, contains vegetable fibre 33%-40 ﹪ in the dried Rhizome of Peltate Yam, starch 45%-55 ﹪, water-soluble material 9%-13 ﹪, saponin content 2%-5 ﹪.Its stem tuber has higher medicinal and chemical industry to be worth, and can extract saponin, two medicine, chemical industry articles for use such as rare through processing, at present can synthesizing hydrogenated cortisone, hundreds of steroid hormone class medicines such as prednisone, dexamethasone.Be the requisite composition of hormone medicine, be called " mother of hormone ", and the class medicines such as analgesia, anesthesia that are widely used in making up, keep healthy, practise contraception, be the comparatively stable medicinal material of a kind of market requirement.The saponin that China produces accounts for global 60 ﹪, ranks first in the world.Production technique mainly is an aging process at present, is about to fresh Chinese yam or dried Chinese yam through washing → pulverizing → spontaneous fermentation → acidolysis → washing → drying → saponin hydrolysate → extraction → crystallization → packing of product.In this traditional technology, need to use the hydrochloric acid soln of 2mol/L-2.5 mol/L, under 130 ℃ of conditions, solid more than 80% is carried out acid hydrolysis, produce 1 ton of saponin and need consume 50 tons of dried medicinal materials of Chinese yam, 19 tons of consumption hydrochloric acid, produce 12 tons in spent acid (more than the sour 0.13N, contain organism 2.8%), high concentrated organic wastewater is more than 1500 tons, and its COD is up to 130000mg/L, BOD is up to 8000 mg/L, pH1.0-2.0.Utilization rate of active components in the Chinese yam is low, and the cost height is big for environment pollution.People have proposed multiple saponin cleaning extraction method for many years, the emphasis of capturing be produce dirt maximum obtain workshop section before the saponin hydrolysate.But because the immature contaminated solution problem or lack various reasons such as economic feasibility and fail all the time to put in the suitability for industrialized production of failing fundamentally of technology.
Summary of the invention
The purpose of this invention is to provide a kind of compound enzymic preparation and the application in diosgenin is produced.
The present invention adopts following technical scheme to realize its goal of the invention, a kind of compound enzymic preparation, it comprises two cover compound enzymic preparations, I type compound enzymic preparation comprises alkaline pectase, cellulase, zytase, hemicellulase, tannase, in every gram I type compound enzymic preparation, the enzyme activity of alkaline pectase is 3000IU/g, the enzyme activity of cellulase is 180IU/g, the enzyme activity of zytase is 6000IU/g, the enzyme activity of hemicellulase is 1500IU/g, and the enzyme activity of tannase is 80IU/g; II type compound enzymic preparation comprises glucase, beta-glucosidase, and in every gram II type compound enzymic preparation, the enzyme activity of glucase is 150IU/g, and the enzyme activity of beta-glucosidase is 120IU/g.
The application of a kind of above-mentioned compound enzymic preparation in diosgenin is produced, it comprises the following steps:
⑴ the pre-treatment of raw material; The silt on flush away Chinese yam surface and other impurity are broken into slurry with bright tapioca flour, maybe dried tapioca flour are broken to 40 orders;
⑵ compound enzymic preparation preparation: I type compound enzymic preparation comprises alkaline pectase, cellulase, zytase, hemicellulase, tannase, in every gram I type compound enzymic preparation, the enzyme activity of alkaline pectase is 3000IU/g, the enzyme activity of cellulase is 180IU/g, the enzyme activity of zytase is 6000IU/g, the enzyme activity of hemicellulase is 1500IU/g, and the enzyme activity of tannase is 80IU/g; II type compound enzymic preparation comprises glucase, beta-glucosidase, and in every gram II type compound enzymic preparation, the enzyme activity of glucase is 150IU/g, and the enzyme activity of beta-glucosidase is 120IU/g;
⑶ a hydrolysis: the adjustment solid-to-liquid ratio is 1:3-6, and the pH value is 6.0-8.5, with the Chinese yam dry weight basis, adds I type compound enzymic preparation 0.6 ﹪-1 ﹪, hydrolysis 10h-14h under 40 ℃ of-50 ℃ of temperature;
⑷ secondary hydrolysis: will transfer pH through the feed liquid that step ⑶ obtains is 5.5-6.0, with the Chinese yam dry weight basis, adds II type compound enzymic preparation 0.1 ﹪-1 ﹪, hydrolysis 1.5h-2h under 60 ℃ of-70 ℃ of temperature;
⑸ the feed liquid that will obtain through step ⑷ after filtration, filter residue washes with water again, filters to make its solid-liquid separation, solid substance is through acidolysis, washing acid, press filtration, after the oven dry saponin hydrolysate; Obtain the saponin finished product by the extraction of saponin extraction common process, crystallization then.
The present invention is with pollutent---waste saccharide liquid is recycled, and in step ⑸, with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, packs behind the high-temperature sterilization.
The present invention is with pollutent---waste saccharide liquid is recycled, and in step ⑸, with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, spraying drying pack the raw sugar powder.
Owing to adopt technique scheme, the present invention has realized goal of the invention preferably, adopt compound enzymic preparation of the present invention to produce diosgenin, can promote the release of yellow ginger effective constituent, improve yield, from solving the used acid of hydrolysis dioscin in essence, total acid molar ratio traditional technology of using reduces more than 70%, thereby reduce production costs, simultaneously can be with pollutent---waste saccharide liquid is recycled, efficiently solve the water pollution problems in the saponin production, the total amount of pollutant reduces more than 85%, reduces the wastewater discharge of saponin manufacturing enterprise 40%.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
A kind of compound enzymic preparation, it comprises two cover compound enzymic preparations, I type compound enzymic preparation comprises alkaline pectase, cellulase, zytase, hemicellulase, tannase, in every gram I type compound enzymic preparation, the enzyme activity of alkaline pectase is 3000IU/g, and the enzyme activity of cellulase is 180IU/g, and the enzyme activity of zytase is 6000IU/g, the enzyme activity of hemicellulase is 1500IU/g, and the enzyme activity of tannase is 80IU/g; II type compound enzymic preparation comprises glucase, beta-glucosidase, and in every gram II type compound enzymic preparation, the enzyme activity of glucase is 150IU/g, and the enzyme activity of beta-glucosidase is 120IU/g.
The present invention has selected two cover compound enzymic preparations targetedly according to the moiety of yellow ginger, and I type compound enzymic preparation is used for loosening and disperses the Chinese yam weave construction, makes the starch in the Chinese yam tissue, pectin, and all kinds of oligose fully dissociate out, and saponin is fully exposed; With II type compound enzymic preparation starch, cellobiose, hemicellulose, pectin etc. are hydrolyzed into water-soluble polysaccharide, pectic acid and glucose again, form the higher and slurries that more easily carry out solid-liquid separation of water-soluble solid content, thereby the slurries that will not contain saponin are collected and are utilized, the solid that will contain saponin is separated for acidolysis, reduce acidolysis thing total amount, make saponin further break away from the Chinese yam weave construction simultaneously, be convenient to acid and directly act on and obtain sapogenin on the saponin, reduce the acidolysis acid concentration.
The application of a kind of compound enzymic preparation as mentioned above in diosgenin is produced, it comprises the following steps:
⑴ the pre-treatment of raw material; The silt on flush away Chinese yam surface and other impurity are broken into slurry with bright tapioca flour, maybe dried tapioca flour are broken to 40 orders;
⑵ compound enzymic preparation preparation: I type compound enzymic preparation comprises alkaline pectase, cellulase, zytase, hemicellulase, tannase, in every gram I type compound enzymic preparation, the enzyme activity of alkaline pectase is 3000IU/g, the enzyme activity of cellulase is 180IU/g, the enzyme activity of zytase is 6000IU/g, the enzyme activity of hemicellulase is 1500IU/g, and the enzyme activity of tannase is 80IU/g; II type compound enzymic preparation comprises glucase, beta-glucosidase, and in every gram II type compound enzymic preparation, the enzyme activity of glucase is 150IU/g, and the enzyme activity of beta-glucosidase is 120IU/g;
⑶ a hydrolysis: adjusting solid-to-liquid ratio is 1:3 for the 1:3-6(present embodiment), the pH value is 6 for the 6.0-8.5(present embodiment), with the Chinese yam dry weight basis, add I type compound enzymic preparation 0.6 ﹪-1 ﹪ (present embodiment is 0.6 ﹪), hydrolysis 10h-14h(present embodiment is 10h under 40 ℃-50 ℃ (present embodiment is 45 ℃) temperature), intermittently starting whipping appts therebetween;
Because contain higher tanning matter in the Chinese yam, this material has stronger restraining effect to enzyme, this material existence can make the functioning efficiency of enzyme descend significantly.Therefore, the present invention adds tannase with the tannin hydrolysis in once-combined enzymic hydrolysis, makes the inhibition ability of its forfeiture to enzyme, helps other each enzyme and plays one's part to the full.
Main component in the Chinese yam is Mierocrystalline cellulose, hemicellulose, starch and xylan etc., and they are to paste or to inlay or covalent linkage chemical bond form bonded mutually.The effect of alkaline pectase is to decompose stickup material-pectin, it is loose that Chinese yam is organized, cell is sowed discord in the tissue, dredges for cellulase, hemicellulase, the zytase of other collapse cell walls and enters the passage and the cells contacting face of Chinese yam tissue, and be beneficial to the dioscin exposure.Cellulase, hemicellulase, zytase enter the Chinese yam tissue fast with the passage of alkaline pectase mediation, and decompose respective substance on the cell walls fast at cell surface, make the cell walls collapse, thereby discharge the starch that is wrapped up by cell walls, saponin, materials such as sugar.For follow-up enzyme continues hydrolyzed starch, sugar creates conditions, also, the acid hydrolysis saponin creates conditions for generating sapogenin.
⑷ secondary hydrolysis: will transfer pH through the feed liquid that step ⑶ obtains is 6 for the 5.5-6.0(present embodiment), with the Chinese yam dry weight basis, add II type compound enzymic preparation 0.1 ﹪-1 ﹪ (present embodiment is 0.1 ﹪), hydrolysis 1.5 h-2h(present embodiment is 1.5h under 60 ℃-70 ℃ (present embodiment is 60 ℃) temperature);
In the secondary combinative enzyme hydrolysis, the present invention is with relay mode continuous enzymolysis Chinese yam tissue and organize releaser.It can continue to be hydrolyzed to glucose with livings starch, the cellobiose that is decomposited by once-combined enzymic hydrolysis to add glucase, β glucuroide, the Chinese yam enzymolysis solution is divided into do not contain the assorted sugared heteroacid solution of saponin and contains loose solid substance two portions of saponin.
⑸ will send filter to filter through the feed liquid that step ⑷ obtains, and filter residue washes twice again with water, filter to make its solid-liquid separation, and solid substance gets saponin hydrolysate through acidolysis, washing acid, press filtration after the oven dry; Obtain the saponin finished product by the extraction of saponin extraction common process, crystallization then.
In traditional technology, waste saccharide liquid and spent acid solution mix, and waste saccharide liquid contains water-insoluble sugar, and the washing amount is big, are difficult to recycle.The present invention is with pollutent---waste saccharide liquid is recycled, in step ⑸, with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, pack behind the high-temperature sterilization, can do raw material for fermentation industry, fodder industry, biological organic fertilizer industry and use.
The present invention adopts enzymolysis to replace the traditional zymotic method, makes the controlled of loose Chinese yam configuration process change, and enzymolysis replaces most of acidolysis, reduces more than hydrochloric acid consumption 70 ﹪ than traditional technology, improves more than the saponin yield 14 ﹪ % in the saponin hydrolysate; And with parent pollutant---waste saccharide liquid is recycled the assorted sugar of the 40-45% that accounts for the Chinese yam dry weight etc., realizes turning waste into wealth cleaner production.The inventive method practicality, effect is remarkable, and the total amount of pollutant is reduced more than 85%, reduces the wastewater discharge of saponin manufacturing enterprise 40%, and saponin quality and output is improved, and production cost descends.
Embodiment 2:
In a hydrolysis of step ⑶, the adjustment solid-to-liquid ratio is 1:6, and the pH value is 6.0, with the Chinese yam dry weight basis, adds I type compound enzymic preparation 1 ﹪, hydrolysis 10h under 45 ℃ of temperature.
In the hydrolysis of step ⑷ secondary, transferring pH is 6.0, with the Chinese yam dry weight basis, adds II type compound enzymic preparation 0.5 ﹪, hydrolysis 1.5 h under 65 ℃ of temperature.
The present invention is with pollutent---waste saccharide liquid is recycled, in step ⑸, with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, spraying drying pack the raw sugar powder, can do raw material for fermentation industry, fodder industry, biological organic fertilizer industry and use.
Surplus with embodiment 1:
Claims (3)
1. the application of compound enzymic preparation in diosgenin is produced is characterized in that it comprises the following steps:
⑴ the pre-treatment of raw material; The silt on flush away Chinese yam surface and other impurity are broken into slurry with bright tapioca flour, maybe dried tapioca flour are broken to 40 orders;
⑵ compound enzymic preparation preparation: I type compound enzymic preparation comprises alkaline pectase, cellulase, zytase, hemicellulase, tannase, in every gram I type compound enzymic preparation, the enzyme activity of alkaline pectase is 3000IU/g, the enzyme activity of cellulase is 180IU/g, the enzyme activity of zytase is 6000IU/g, the enzyme activity of hemicellulase is 1500IU/g, and the enzyme activity of tannase is 80IU/g; II type compound enzymic preparation comprises glucase, beta-glucosidase, and in every gram II type compound enzymic preparation, the enzyme activity of glucase is 150IU/g, and the enzyme activity of beta-glucosidase is 120IU/g;
⑶ a hydrolysis: the adjustment solid-to-liquid ratio is 1:3-6, and the pH value is 6.0-8.5, with the Chinese yam dry weight basis, adds I type compound enzymic preparation 0.6 ﹪-1 ﹪, hydrolysis 10h-14h under 40 ℃ of-50 ℃ of temperature;
⑷ secondary hydrolysis: will transfer pH through the feed liquid that step ⑶ obtains is 5.5-6.0, with the Chinese yam dry weight basis, adds II type compound enzymic preparation 0.1 ﹪-1 ﹪, hydrolysis 1.5h-2h under 60 ℃ of-70 ℃ of temperature;
⑸ the feed liquid that will obtain through step ⑷ after filtration, filter residue washes with water again, filters to make its solid-liquid separation, solid substance is through acidolysis, washing acid, press filtration, after the oven dry saponin hydrolysate; Obtain the saponin finished product by the extraction of saponin extraction common process, crystallization then.
2. the application of compound enzymic preparation according to claim 1 in diosgenin is produced is characterized in that in step ⑸, and with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, packs behind the high-temperature sterilization.
3. the application of compound enzymic preparation according to claim 1 in diosgenin is produced is characterized in that in step ⑸, and with the filtrate filter-press water-washing, filtrate concentrates through step-down, when solid in the concentrated solution reaches 40 ﹪～45 ﹪, spraying drying pack the raw sugar powder.
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| CN101857855B true CN101857855B (en) | 2011-12-07 |
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| CN105039296A (en) * | 2015-06-10 | 2015-11-11 | 嘉兴卓盛生物科技有限公司 | Turmeric saponin compound enzyme reagent, preparation method thereof and method of utilizing same to prepare turmeric saponin |
| CN105112487A (en) * | 2015-09-16 | 2015-12-02 | 张家界久瑞生物科技有限公司 | Method for extracting dioscorea zingiberensis saponin by utilizing biological enzyme fermentation technology |
| CN109913434A (en) * | 2019-04-08 | 2019-06-21 | 宁夏夏盛实业集团有限公司 | A kind of complex enzyme and its preparation method and application |
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| CN101397582A (en) * | 2007-09-30 | 2009-04-01 | 咸阳宏华生化科技有限公司 | Method for producing diosgenin by environment friendly pollution-free holoenzyme method |
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