CN101386845A - Complex enzyme preparation for turmeric industry and method of use thereof - Google Patents
Complex enzyme preparation for turmeric industry and method of use thereof Download PDFInfo
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- CN101386845A CN101386845A CNA2008101743754A CN200810174375A CN101386845A CN 101386845 A CN101386845 A CN 101386845A CN A2008101743754 A CNA2008101743754 A CN A2008101743754A CN 200810174375 A CN200810174375 A CN 200810174375A CN 101386845 A CN101386845 A CN 101386845A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
The invention relates to a turmeric complex enzyme preparation for industrial use and an application method thereof. The complex enzyme preparation is characterized by being prepared from complex cellulase, pectinase, glucanase, amylase, glycosidase and phytase by compounding. Based on the compositions of turmeric, the enzymes are selected directionally for compounding. In the invention, the complex enzyme preparation is adopted to produce diosgenin, which can promote the release of active ingredients of the turmeric, improve the yield, and solve acid for the hydrolysis of dioscin essentially, thereby lowering the production cost, simultaneously solving the water pollution problem in the saponin production effectively, and reducing 40 percent of the wastewater discharge from turmeric production enterprises.
Description
Technical field
The present invention relates to the biological enzyme formulation technical field, particularly relate to a kind of complex enzyme preparation for turmeric industry and application method thereof.
Background technology
Yellow ginger has another name called Dioscorea nipponica Mak. Ningpo Yam Rhizome, the formal name used at school Rhizome of Peltate Yam, be the herbaceous perennial vine plant, its stem tuber has higher medicinal and chemical industry to be worth, can extract saponin, two medicine such as rare, chemical industry articles for use through processing, especially the diosgenin that extracts from yellow ginger is the basic material medicine of synthesizing steroid class medicine, utilize it can synthesizing hydrogenated cortisone, hundreds of steroid hormone class medicine such as prednisone, dexamethasone.Therefore yellow ginger is called " mother of hormone ", is the requisite composition of hormone medicine, and the class medicines such as analgesia, anesthesia that are widely used in making up, keep healthy, practise contraception, and is the comparatively stable medicinal material of a kind of market requirement.Traditional technology of extracting diosgenin from yellow ginger is Chinese yam to be pulverized the back complete feed drop into acidolysis, but the utilization rate of active components that this method exists in the Chinese yam is low, the cost height, defective such as big for environment pollution, the dried medicinal material of common more than 50 tons of Chinese yams only can be produced 1 ton of saponin, and its consumption hydrochloric acid reaches about 19 tons, washes the acidolysis thing with water to neutral, produce (more than sour 0.13 equivalent, containing organism 2.8%) high concentrated organic wastewater up to 1500 tons about.
Summary of the invention
Purpose of the present invention just is the defective that overcomes above-mentioned prior art is provided, and a kind of release that impels yellow ginger effective constituent is provided, and improves yield, reduces the saponin production cost, realizes that saponin cleans the complex enzyme preparation for turmeric industry of production.
Another object of the present invention is the concrete application method of above-mentioned complex enzyme preparation for turmeric industry.
The present invention is achieved through the following technical solutions:
A kind of complex enzyme preparation for turmeric industry is characterized in that being made up of by weight percentage following compositions:
Complex cellulase 20%-30%,
Polygalacturonase 12%-25%,
Dextranase 10%-15%,
Amylase 25%-35%,
Glycosylase 5%-10%,
Phytase 3%-5%;
Described complex cellulase is made up of 50% cellulase and 50% zytase, by weight percentage;
Described polygalacturonase is made up of 60% Rohapect MPE and 40% pectin lyase, by weight percentage;
Described amylase is made up of 70% middle temperature α-Dian Fenmei, 20% fungal alpha-amylase and 10% Pullulanase, by weight percentage;
Described Glycosylase is 60% to be made up of beta-glucosidase and 40% rhamnosidase, by weight percentage;
Described dextranase is the beta-glucan enzyme.
The application method of above-mentioned complex enzyme preparation for turmeric industry, its processing step is:
A. raw material is through the combined-enzyme method pre-treatment: the impurity elimination of cadmium yellow ginger, add the broken pulping of triplication water with beater disintegrating machine, and add 5/10000ths to 5/1000ths complex enzyme preparation for turmeric industry, enzymolysis is 1-6 hours under room temperature, natural pH value condition,
B. organic solvent extraction: add the ethanol of 30-80% in the raw material behind enzymolysis,, extracted 1-6 hours in volume percent, solid-liquid separation, the material slag repeats extraction once,
C. reclaim solvent, the extract drying: reclaim ethanol, extract is dried at 30-80 ℃,
D. the complex enzyme hydrolysis saponin(e is an aglycon: extract dilutes with 3 times of water gagings, adds 5/10000ths to 5/1000ths complex enzyme preparation for turmeric industry, at 42 ℃-60 ℃, and pH value 4.5-6.5 enzymolysis 2-40 hour,
E. dry zymolyte: after the zymolyte oven dry, extract by saponin that common process extracts, concentrates, crystallization obtains the saponin finished product.
Detection shows: contain vegetable fibre 33%-40% in the Rhizoma Dioscoreae Zingiberensis, starch 45%-55%, water-soluble material 9%-13%, saponin content 2%-3%, therefore, the selection of the property of the present invention is directed to cellulase, polygalacturonase, dextranase, amylase, phytase and Glycosylase, their action principle is as follows:
1. enough complex cellulases: the Mierocrystalline cellulose in can hydrolysis material, destroy the skein cell wall construction that its chain-like structure and hemicellulose thereof constitute, discharge the effective constituent saponin(e that wherein is wrapped.
2. enough polygalacturonases and beta-glucanase: by the synergy of polygalacturonase and beta-glucanase, degraded causes the physical channel obstruction to cause moisture to ooze out from the molecular channel because of the existence of pectin in the plant materials and dextran, cause extract viscosity big, open intermolecular passage by the turmeric industry prozyme, the degraded sticky contaminant reduces and filters viscosity.
3. enough amylase: starch content is 45%-55% in the yellow ginger, and yellow ginger is pulverized the back dioscin by a large amount of starch parcels, and amylase can help the extraction of organic solvent to saponin(e with starch liquefacation.
4. an amount of phytase: collaborative complex cellulase and polygalacturonase acting in conjunction can change most of colloidalmaterials such as pectin substance, the power that attracts each other of positive charge in the fiber of possess hydrophilic property matter and half fiber surface electric charge and the water in plant cell wall.
5. an amount of Glycosylase: the glycosidic link in can the hydrolysis dioscin is used for the bio-transformation of saponin(e.
Processing parameter by the complex enzyme preparation for turmeric industry that aforesaid combination obtained is:
Operative temperature: 15-60 ℃ of (or room temperature) pH value: 4.0-7.0 (or natural pH)
Action time: 2-48h addition: 500g-5000g/ ton material
Stir: 40% backflow mortise liquid stirring action finishes pH value: 3.0-3.5
The present invention is used for above-mentioned compound enzymic preparation the production of diosgenin, it is different from traditional technology the Chinese yam full dose is dropped into acidolysis, cause yield low, the production cost height, the drawbacks such as environmental pollution that large amount of sewage brings, also be different from several novel procesies Mechanical Crushing Chinese yam cell walls, percentage of damage is low, the high defective that consumes energy, and this processing method adopts continuous combined-enzyme method, can promote the release of yellow ginger effective constituent, improve yield, from solving the used acid of hydrolysis dioscin in essence, thereby reduce production costs, efficiently solve the water pollution problems in the saponin production simultaneously, reduce the wastewater discharge of yellow ginger manufacturing enterprise 40%.
Using the present invention can reduce enterprise's production cycle greatly, and the waste water after the yellow ginger hydrolysis can be used for fermentation alcohol, improves comprehensive utilization ratio.
Use the waste reside comprehensive utilization after the present invention can extract yellow ginger saponin, be used for perfect compound feed, reduce enterprise's production cost, increase yield.
Embodiment
Embodiment 1
The concrete prescription of complex enzyme preparation for turmeric industry of the present invention is as follows, by weight percentage: complex cellulase 30% (wherein cellulase 50%, zytase 50%), (wherein Rohapect MPE 60% for polygalacturonase 15%, pectin lyase 40%), amylase 25% (warm α-Dian Fenmei 70% wherein, fungal alpha-amylase 20%, Pullulanase 10%), beta-glucan enzyme 15%, Glycosylase 10% (wherein beta-glucosidase 60%, rhamnosidase 40%), phytase 5%.
The preparation method is: respectively through micro-filtrate membrane filtration, it is composite to enter automatic mixing machine more in proportion with dhdps enzyme, by smart membrane filtration, quantitative filling, becomes product then.
Embodiment 2
The concrete prescription of complex enzyme preparation for turmeric industry of the present invention is as follows, by weight percentage: complex cellulase 25% (wherein cellulase 50%, zytase 50%), (wherein Rohapect MPE 60% for polygalacturonase 25%, pectin lyase 40%), amylase 35% (warm α-Dian Fenmei 70% wherein, fungal alpha-amylase 20%, Pullulanase 10%), beta-glucan enzyme 10%, Glycosylase 5% (wherein beta-glucosidase 60%, rhamnosidase 40%), phytase 5%.
The preparation method is identical with example 1.
Embodiment 3
The concrete prescription of complex enzyme preparation for turmeric industry of the present invention is as follows, by weight percentage: complex cellulase 20% (wherein cellulase 50%, zytase 50%), (wherein Rohapect MPE 60% for polygalacturonase 20%, pectin lyase 40%), amylase 35% (warm α-Dian Fenmei 70% wherein, fungal alpha-amylase 20%, Pullulanase 10%), beta-glucan enzyme 14%, Glycosylase 8% (wherein beta-glucosidase 60%, rhamnosidase 40%), phytase 3%.
The preparation method is identical with example 1.
Embodiment 4
The concrete prescription of complex enzyme preparation for turmeric industry of the present invention is as follows, by weight percentage: complex cellulase 28% (wherein cellulase 50%, zytase 50%), (wherein Rohapect MPE 60% for polygalacturonase 12%, pectin lyase 40%), amylase 30% (warm α-Dian Fenmei 70% wherein, fungal alpha-amylase 20%, Pullulanase 10%), beta-glucan enzyme 15%, Glycosylase 10% (wherein beta-glucosidase 60%, rhamnosidase 40%), phytase 5%.
The preparation method is identical with example 1.
Embodiment 1
The application method of above-mentioned complex enzyme preparation for turmeric industry, its processing step is:
A. raw material combined-enzyme method pre-treatment: bright Chinese yam impurity elimination, add the broken pulping of triplication water with beater disintegrating machine, the complex enzyme preparation for turmeric industry of adding 5/10000ths to 5/1000ths, enzymolysis is 1-6 hours under room temperature, natural pH value condition, the purpose of this process is to destroy plant cell wall by biological means, discharge dioscin, the degraded macromolecular substance
B. organic solvent extraction: add the ethanol of 30-80% in the raw material behind enzymolysis,, extracted 1-6 hours in volume percent, solid-liquid separation, the material slag repeats extraction once.
C. reclaim solvent, the extract drying: reclaim ethanol, extract is dried at 30-80 ℃,
D. the complex enzyme hydrolysis saponin(e is an aglycon: extract dilutes with 3 times of water gagings, adds 5/10000ths to 5/1000ths compound enzymic preparation, at 42 ℃-60 ℃, pH value 4.5-6.5 enzymolysis 2-40 hour, this process can make saponin(e be converted into sapogenin, and the transformation efficiency of glycosides and aglycon reaches more than 90%
E. dry zymolyte: after the zymolyte oven dry, extract by saponin that common process extracts, concentrates, crystallization obtains the saponin finished product.
Claims (7)
1. complex enzyme preparation for turmeric industry is characterized in that being made up of by weight percentage following compositions:
Complex cellulase 20%-30%,
Polygalacturonase 12%-25%,
Dextranase 10%-15%,
Amylase 25%-35%,
Glycosylase 5%-10%,
Phytase 3%-5%.
2,, it is characterized in that described complex cellulase is made up of 50% cellulase and 50% zytase, by weight percentage according to the described complex enzyme preparation for turmeric industry of claim 1.
3,, it is characterized in that described polygalacturonase is made up of 60% Rohapect MPE and 40% pectin lyase, by weight percentage according to the described complex enzyme preparation for turmeric industry of claim 1.
4,, it is characterized in that described amylase is made up of 70% middle temperature α-Dian Fenmei, 20% fungal alpha-amylase and 10% Pullulanase, by weight percentage according to the described complex enzyme preparation for turmeric industry of claim 1.
5,, it is characterized in that described Glycosylase is 60% to be made up of beta-glucosidase and 40% rhamnosidase, by weight percentage according to the described complex enzyme preparation for turmeric industry of claim 1.
6,, it is characterized in that described dextranase is the beta-glucan enzyme according to the described complex enzyme preparation for turmeric industry of claim 1.
7, a kind of application method of complex enzyme preparation for turmeric industry as claimed in claim 1, its processing step is:
A. raw material is through the combined-enzyme method pre-treatment: the impurity elimination of cadmium yellow ginger, add the broken pulping of triplication water with beater disintegrating machine, and add 5/10000ths to 5/1000ths complex enzyme preparation for turmeric industry, enzymolysis is 1-6 hours under room temperature, natural pH value condition,
B. organic solvent extraction: add the ethanol of 30-80% in the raw material behind enzymolysis,, extracted 1-6 hours in volume percent, solid-liquid separation, the material slag repeats extraction once,
C. reclaim solvent, the extract drying: reclaim ethanol, extract is dried at 30-80 ℃,
D. the complex enzyme hydrolysis saponin(e is an aglycon: extract dilutes with 3 times of water gagings, adds 5/10000ths to 5/1000ths complex enzyme preparation for turmeric industry, at 42 ℃-60 ℃, and pH value 4.5-6.5 enzymolysis 2-40 hour,
E. dry zymolyte: after the zymolyte oven dry, extract by saponin that common process extracts, concentrates, crystallization obtains the saponin finished product.
Priority Applications (1)
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| CNA2008101743754A CN101386845A (en) | 2008-10-31 | 2008-10-31 | Complex enzyme preparation for turmeric industry and method of use thereof |
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| CNA2008101743754A CN101386845A (en) | 2008-10-31 | 2008-10-31 | Complex enzyme preparation for turmeric industry and method of use thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478411A (en) * | 2013-09-16 | 2014-01-01 | 陕西科技大学 | Turmeric starch probiotics activity feed and preparing method thereof |
| CN105039296A (en) * | 2015-06-10 | 2015-11-11 | 嘉兴卓盛生物科技有限公司 | Turmeric saponin compound enzyme reagent, preparation method thereof and method of utilizing same to prepare turmeric saponin |
| CN105062990A (en) * | 2015-07-27 | 2015-11-18 | 天津科建科技发展有限公司 | Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation |
| CN105801665A (en) * | 2016-04-22 | 2016-07-27 | 湖南农业大学 | Method for extracting protodioscin from dioscorea opposita plant |
| CN105925559A (en) * | 2016-06-23 | 2016-09-07 | 宁夏乙征生物工程有限公司 | Method for preparing immobilized enzyme and hydrolyzing diosgenin |
| CN106086150A (en) * | 2016-07-25 | 2016-11-09 | 陕西科技大学 | A kind of method utilizing enzyme to combine microorganism conversion production turmeric saponin |
| CN106191010A (en) * | 2016-09-27 | 2016-12-07 | 郑州轻工业学院 | A kind of rhamnosidase and hydrolysis Dioscorea zingiberensis dioscin prepare the application in diosgenin |
| CN110938612A (en) * | 2018-09-25 | 2020-03-31 | 中国医学科学院医药生物技术研究所 | Method for preparing diosgenin by using biological enzyme and biological material used by same |
| CN110938613A (en) * | 2018-09-25 | 2020-03-31 | 中国医学科学院医药生物技术研究所 | Glycosyl hydrolase and related biological material and application thereof |
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2008
- 2008-10-31 CN CNA2008101743754A patent/CN101386845A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478411A (en) * | 2013-09-16 | 2014-01-01 | 陕西科技大学 | Turmeric starch probiotics activity feed and preparing method thereof |
| CN105039296A (en) * | 2015-06-10 | 2015-11-11 | 嘉兴卓盛生物科技有限公司 | Turmeric saponin compound enzyme reagent, preparation method thereof and method of utilizing same to prepare turmeric saponin |
| CN105062990A (en) * | 2015-07-27 | 2015-11-18 | 天津科建科技发展有限公司 | Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation |
| CN105801665A (en) * | 2016-04-22 | 2016-07-27 | 湖南农业大学 | Method for extracting protodioscin from dioscorea opposita plant |
| CN105801665B (en) * | 2016-04-22 | 2017-11-17 | 湖南农业大学 | A kind of method that protodioscin is extracted in the plant from Chinese yam |
| CN105925559A (en) * | 2016-06-23 | 2016-09-07 | 宁夏乙征生物工程有限公司 | Method for preparing immobilized enzyme and hydrolyzing diosgenin |
| CN105925559B (en) * | 2016-06-23 | 2020-09-01 | 宁夏乙征生物工程有限公司 | Preparation of immobilized enzyme and method for hydrolyzing diosgenin |
| CN106086150A (en) * | 2016-07-25 | 2016-11-09 | 陕西科技大学 | A kind of method utilizing enzyme to combine microorganism conversion production turmeric saponin |
| CN106191010A (en) * | 2016-09-27 | 2016-12-07 | 郑州轻工业学院 | A kind of rhamnosidase and hydrolysis Dioscorea zingiberensis dioscin prepare the application in diosgenin |
| CN110938612A (en) * | 2018-09-25 | 2020-03-31 | 中国医学科学院医药生物技术研究所 | Method for preparing diosgenin by using biological enzyme and biological material used by same |
| CN110938613A (en) * | 2018-09-25 | 2020-03-31 | 中国医学科学院医药生物技术研究所 | Glycosyl hydrolase and related biological material and application thereof |
| CN110938612B (en) * | 2018-09-25 | 2021-06-15 | 中国医学科学院医药生物技术研究所 | Method for preparing diosgenin by using biological enzyme and biological material used by same |
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