CN101709151A - Method for extracting dioscorea zingiberensis pigment by enzymatic method - Google Patents
Method for extracting dioscorea zingiberensis pigment by enzymatic method Download PDFInfo
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- CN101709151A CN101709151A CN200910219186A CN200910219186A CN101709151A CN 101709151 A CN101709151 A CN 101709151A CN 200910219186 A CN200910219186 A CN 200910219186A CN 200910219186 A CN200910219186 A CN 200910219186A CN 101709151 A CN101709151 A CN 101709151A
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Abstract
The invention disclose a method for extracting dioscorea zingiberensis pigment by an enzymatic method, which comprises the following steps of: dissolving pectinase in an acetic acid-sodium acetate solution to prepare a pectinase enzymatic solution; adding dioscorea zingiberensis powder into the pectinase enzymatic solution, then adding the acetic acid-sodium acetate solution for reacting, and adding anhydrous ethyl alcohol; extracting in thermostatic waterbath with the temperature of 65-70 DEG C; repeatedly extracting; merging filtrate; and then concentrating the filtrate to that the concentration of ethyl alcohol is less than 10 percent to obtain the dioscorea zingiberensis pigment. By utilizing the special property of the pectinase, the invention effectively improves pigment extraction amount without environmental pollution during production and lays a foundation for the scale production of the dioscorea zingiberensis pigment. By adopting enzyme to pretreat the dioscorea zingiberensis for damaging cell structures and degrading impurities, the invention has higher pigment obtaining efficiency and pigment quality, and not only alleviates environmental pollution and improves the utilization rate of the dioscorea zingiberensis, but also explores a novel edible natural pigment.
Description
Technical field
The present invention relates to a kind of extracting method of dioscorea zingiberensis pigment, be specifically related to a kind of method of method for extracting dioscorea zingiberensis pigment by enzymatic.
Background technology
Yellow ginger has another name called Rhizome of Peltate Yam (Dioscorea zingiberensis), the herbaceous species plant, and it is yellow or orange that its rhizome is, and contains starch, tannin, alkaloid, pigment, saponin etc., is a classical medicinal material.Yellow ginger is as medicinal and edible long history arranged all at home and abroad, its pigment belongs to the row of natural pigment, but people give great concern to extract the medicine intermediate saponin from yellow ginger always for a long time, and it is few to the report of the development and use of dioscorea zingiberensis pigment, most of saponin manufacturing enterprise with dioscorea zingiberensis pigment with discharge of wastewater, chroma in waste water high pollution environment not only, and caused the wasting of resources, therefore dioscorea zingiberensis pigment is worth research and development.This can be industry such as food and new natural pigment is provided and increases the comprehensive utilization ratio of saponin manufacturing enterprise to yellow ginger, thereby improves the income of saponin manufacturing enterprise and reduce environmental pollution.
The prior art of dioscorea zingiberensis pigment extraction at present has: Tang Xuan (Study on extraction of yellow pigment in the yellow ginger) etc. adopt yellow pigment in the organic solvent extraction yellow ginger; Liu Jianben (extraction of yellow pigment and Study on Stability in the yellow ginger) adopts the organic solvent extraction yellow pigment and pigment stability is studied; Existing dioscorea zingiberensis pigment extracting method is the organic solvent extraction method, and extraction efficiency is not high.
Summary of the invention
The object of the present invention is to provide a kind of easy, method of method for extracting dioscorea zingiberensis pigment by enzymatic efficiently.
For achieving the above object, first kind of technical scheme that the present invention adopts is:
At first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
Secondly, the polygalacturonase of getting 60mg is that acetate-sodium acetate buffer solution of 3.6～4.4 is settled to 10mL with the pH value, is mixed with the polygalacturonase enzyme liquid of 6mg/mL;
Polygalacturonase assisted extraction dioscorea zingiberensis pigment: get 1.000g yellow ginger powder, add the above-mentioned polygalacturonase enzyme of 1.5mL liquid, and be that acetate-sodium acetate buffer solution of 3.6～4.4 complements to 8mL with the pH value, react 3～5h down at 40～50 ℃, add the 12mL dehydrated alcohol then, 2～2.5h are extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Second kind of technical scheme that the present invention adopts is:
At first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
Secondly, getting 50～70mg cellulase and hemicellulase respectively, is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to 10mL with the pH value, is mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5～7mg/mL respectively;
The compound extraction dioscorea zingiberensis pigment of Mierocrystalline cellulose and hemicellulase: get 1.000g yellow ginger powder, the cellulose enzyme liquid and the hemicellulase enzyme liquid that add 1.0mL respectively, and be that acetate-sodium acetate buffer solution of 4.6～5.4 complements to 8mL with the pH value, react 2～3h down at 40～50 ℃, add the 12mL dehydrated alcohol then, 2～2.5h is extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
The third technical scheme that the present invention adopts is:
At first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
Secondly, get 50～70mg cellulase and hemicellulase respectively, with the pH value is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to 10mL, be mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5～7mg/mL respectively, getting 40～50mg polygalacturonase again, is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to the polygalacturonase enzyme liquid that 10mL is mixed with 4～5mg/mL with the pH value again;
The compound extraction dioscorea zingiberensis pigment of cellulase, hemicellulase and polygalacturonase: get 1.000g yellow ginger powder, add 1.0mL cellulose enzyme liquid, hemicellulase enzyme liquid and 2.0mL polygalacturonase enzyme liquid respectively, and be that acetate-sodium acetate buffer solution of 4.6～5.4 complements to 8mL with the pH value, react 3.5～4.5h down at 50～60 ℃, add the 12mL dehydrated alcohol then, 2～2.5h is extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
The present invention utilizes the ins and outs of cellulase, hemicellulase and polygalacturonase, effectively improves the extracted amount of pigment, and the production process environmentally safe, for the large-scale production dioscorea zingiberensis pigment lays the foundation.Adopt enzyme to the yellow ginger pre-treatment, destroy cellularstructure and degradation impurity, make gained pigment efficient and quality higher, not only alleviate problem of environmental pollution, improve the yellow ginger comprehensive utilization ratio, can also develop a kind of novel edible natural pigment.Because cellulase belongs to multi-component enzyme, except that cellulase, still contain hemicellulase, β-1,4-dextranase, zytase, compositions such as cellobiase are because of the effective constituent of plant is wrapped in the cell walls mostly, to the extraction of these effective constituents, be subjected to the cellulosic obstruction of cell walls main component, often extraction efficiency is lower.Cellulase can decompose plant cell wall, generates cell-oligosaccharide, cellobiose and glucose, thereby changes the permeability of cell walls, the release and the utilization that improve active ingredients of plants greatly.Zytase can decompose plant material cell walls and beta-glucan, reduces the viscosity of material, promotes the release of active substance.Polygalacturonase is the polygalacturonic acid lytic enzyme in essence, and hydrolysis of pectin mainly generates beta galactose aldehydic acid.Polygalacturonase is mainly used in squeezing the juice and clarifying of juice drinks and fruit wine, and decompose pectin is had good effect.
Description of drawings
Fig. 1 is that polygalacturonase extracts the dioscorea zingiberensis pigment uv-spectrogram.
Embodiment
Embodiment 1: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, the polygalacturonase of getting 60mg is that acetate-sodium acetate buffer solution of 3.6 is settled to 10mL with the pH value, is mixed with the polygalacturonase enzyme liquid of 6mg/mL; Get 1.000g yellow ginger powder, add the above-mentioned polygalacturonase enzyme of 1.5mL liquid, and be that acetate-sodium acetate buffer solution of 3.6 complements to 8mL with the pH value, react 5h down at 40 ℃, add the 12mL dehydrated alcohol then, 2.5h are extracted in 65 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.Fig. 1 be the dioscorea zingiberensis pigment of present embodiment gained through dilution gained uv-spectrogram, the absorption peak of pigment is at 275nm.
Embodiment 2: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, the polygalacturonase of getting 60mg is that acetate-sodium acetate buffer solution of 4.0 is settled to 10mL with the pH value, is mixed with the polygalacturonase enzyme liquid of 6mg/mL; Get 1.000g yellow ginger powder, add the above-mentioned polygalacturonase enzyme of 1.5mL liquid, and be that acetate-sodium acetate buffer solution of 4.0 complements to 8mL with the pH value, react 4h down at 45 ℃, add the 12mL dehydrated alcohol then, 2.3h are extracted in 68 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 3: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, the polygalacturonase of getting 60mg is that acetate-sodium acetate buffer solution of 4.4 is settled to 10mL with the pH value, is mixed with the polygalacturonase enzyme liquid of 6mg/mL; Get 1.000g yellow ginger powder, add the above-mentioned polygalacturonase enzyme of 1.5mL liquid, and be that acetate-sodium acetate buffer solution of 4.4 complements to 8mL with the pH value, react 3h down at 50 ℃, add the 12mL dehydrated alcohol then, 2h are extracted in 70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 4: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, getting 50mg cellulase and hemicellulase respectively, is that acetate-sodium acetate buffer solution of 4.6 is settled to 10mL with the pH value respectively, is mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5mg/mL; Get 1.000g yellow ginger powder, the cellulose enzyme liquid and the hemicellulase enzyme liquid that add 1.0mL respectively, and be that acetate-sodium acetate buffer solution of 4.6 complements to 8mL with the pH value, react 3h down at 40 ℃, add the 12mL dehydrated alcohol then, 2.5h are extracted in 65 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 5: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, getting 60mg cellulase and hemicellulase respectively, is that acetate-sodium acetate buffer solution of 5.0 is settled to 10mL with the pH value respectively, is mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 6mg/mL; Get 1.000g yellow ginger powder, the cellulose enzyme liquid and the hemicellulase enzyme liquid that add 1.0mL respectively, and be that acetate-sodium acetate buffer solution of 5.0 complements to 8mL with the pH value, react 2.5h down at 45 ℃, add the 12mL dehydrated alcohol then, 2.3h are extracted in 68 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 6: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, getting 70mg cellulase and hemicellulase respectively, is that acetate-sodium acetate buffer solution of 5.4 is settled to 10mL with the pH value respectively, is mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 7mg/mL; Get 1.000g yellow ginger powder, the cellulose enzyme liquid and the hemicellulase enzyme liquid that add 1.0mL respectively, and be that acetate-sodium acetate buffer solution of 5.4 complements to 8mL with the pH value, react 2h down at 50 ℃, add the 12mL dehydrated alcohol then, 2h are extracted in 70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 7: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, get 50mg cellulase and hemicellulase respectively, be that acetate-sodium acetate buffer solution of 4.6 is settled to 10mL with the pH value respectively, be mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5mg/mL, getting the 40mg polygalacturonase again, is that acetate-sodium acetate buffer solution of 4.6 is settled to the polygalacturonase enzyme liquid that 10mL is mixed with 4mg/mL with the pH value again; Get 1.000g yellow ginger powder, add 1.0mL cellulose enzyme liquid, hemicellulase enzyme liquid and 2.0mL polygalacturonase enzyme liquid respectively, and be that acetate-sodium acetate buffer solution of 4.6 complements to 8mL with the pH value, react 4.5h down at 50 ℃, add the 12mL dehydrated alcohol then, 2.5h are extracted in 65 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 8: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, get 60mg cellulase and hemicellulase respectively, be that acetate-sodium acetate buffer solution of 5.0 is settled to 10mL with the pH value respectively, be mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 6mg/mL, getting the 45mg polygalacturonase again, is that acetate-sodium acetate buffer solution of 5.0 is settled to the polygalacturonase enzyme liquid that 10mL is mixed with 4.5mg/mL with the pH value again; Get 1.000g yellow ginger powder, add 1.0mL cellulose enzyme liquid, hemicellulase enzyme liquid and 2.0mL polygalacturonase enzyme liquid respectively, and be that acetate-sodium acetate buffer solution of 5.0 complements to 8mL with the pH value, react 4.0h down at 55 ℃, add the 12mL dehydrated alcohol then, 2.3h are extracted in 68 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Embodiment 9: at first, get the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves; Secondly, get 70mg cellulase and hemicellulase respectively, be that acetate-sodium acetate buffer solution of 5.4 is settled to 10mL with the pH value respectively, be mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 7mg/mL, getting the 50mg polygalacturonase again, is that acetate-sodium acetate buffer solution of 5.4 is settled to the polygalacturonase enzyme liquid that 10mL is mixed with 5mg/mL with the pH value again; Get 1.000g yellow ginger powder, add 1.0mL cellulose enzyme liquid, hemicellulase enzyme liquid and 2.0mL polygalacturonase enzyme liquid respectively, and be that acetate-sodium acetate buffer solution of 5.4 complements to 8mL with the pH value, react 3.5h down at 60 ℃, add the 12mL dehydrated alcohol then, 2h are extracted in 70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
Claims (3)
1. the method for method for extracting dioscorea zingiberensis pigment by enzymatic is characterized in that:
1) at first, gets the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
2) secondly, the polygalacturonase of getting 60mg is that acetate-sodium acetate buffer solution of 3.6～4.4 is settled to 10mL with the pH value, is mixed with the polygalacturonase enzyme liquid of 6mg/mL;
3) polygalacturonase assisted extraction dioscorea zingiberensis pigment: get 1.000g yellow ginger powder, add the above-mentioned polygalacturonase enzyme of 1.5mL liquid, and be that acetate-sodium acetate buffer solution of 3.6～4.4 complements to 8mL with the pH value, react 3～5h down at 40～50 ℃, add the 12mL dehydrated alcohol then, 2～2.5h are extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
2. the method for method for extracting dioscorea zingiberensis pigment by enzymatic is characterized in that:
1) at first, gets the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
2) secondly, getting 50～70mg cellulase and hemicellulase respectively, is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to 10mL with the pH value, is mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5～7mg/mL respectively;
3) the compound extraction dioscorea zingiberensis pigment of Mierocrystalline cellulose and hemicellulase: get 1.000g yellow ginger powder, the cellulose enzyme liquid and the hemicellulase enzyme liquid that add 1.0mL respectively, and be that acetate-sodium acetate buffer solution of 4.6～5.4 complements to 8mL with the pH value, react 2～3h down at 40～50 ℃, add the 12mL dehydrated alcohol then, 2～2.5h is extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
3. the method for method for extracting dioscorea zingiberensis pigment by enzymatic is characterized in that:
1) at first, gets the dry back of also pulverizing of cadmium yellow ginger and cross 60 mesh sieves;
2) secondly, get 50～70mg cellulase and hemicellulase respectively, with the pH value is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to 10mL, be mixed with cellulose enzyme liquid and the hemicellulase enzyme liquid of 5～7mg/mL respectively, getting 40～50mg polygalacturonase again, is that acetate-sodium acetate buffer solution of 4.6～5.4 is settled to the polygalacturonase enzyme liquid that 10mL is mixed with 4～5mg/mL with the pH value again;
4) the compound extraction dioscorea zingiberensis pigment of cellulase, hemicellulase and polygalacturonase: get 1.000g yellow ginger powder, add 1.0mL cellulose enzyme liquid, hemicellulase enzyme liquid and 2.0mL polygalacturonase enzyme liquid respectively, and be that acetate-sodium acetate buffer solution of 4.6～5.4 complements to 8mL with the pH value, react 3.5～4.5h down at 50～60 ℃, add the 12mL dehydrated alcohol then, 2～2.5h is extracted in 65～70 ℃ of waters bath with thermostatic control, wherein solid-liquid ratio is 1g: 20mL, extract repeatedly 2～3 times, merging filtrate is concentrated into filtrate alcohol concn again and obtains dioscorea zingiberensis pigment less than 10%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104312198A (en) * | 2014-09-30 | 2015-01-28 | 于芳 | Method for extracting yellow pigment from jackfruit peel |
| CN106084879A (en) * | 2016-07-15 | 2016-11-09 | 广西顺帆投资有限公司 | A kind of based on the method freezing green extraction dyestuff |
| CN106084878A (en) * | 2016-07-15 | 2016-11-09 | 广西顺帆投资有限公司 | A kind of method extracting dyestuff based on Semen Momordicae fruit |
| CN106280540A (en) * | 2016-07-25 | 2017-01-04 | 台江县南宫乡大田村蓝靛种植专业合作社 | A kind of production method of vegetable colour |
-
2009
- 2009-11-27 CN CN200910219186A patent/CN101709151A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104312198A (en) * | 2014-09-30 | 2015-01-28 | 于芳 | Method for extracting yellow pigment from jackfruit peel |
| CN106084879A (en) * | 2016-07-15 | 2016-11-09 | 广西顺帆投资有限公司 | A kind of based on the method freezing green extraction dyestuff |
| CN106084878A (en) * | 2016-07-15 | 2016-11-09 | 广西顺帆投资有限公司 | A kind of method extracting dyestuff based on Semen Momordicae fruit |
| CN106280540A (en) * | 2016-07-25 | 2017-01-04 | 台江县南宫乡大田村蓝靛种植专业合作社 | A kind of production method of vegetable colour |
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Application publication date: 20100519 |