CN101849019A - 基于其nad依赖性dna连接酶活性来检测微生物 - Google Patents

基于其nad依赖性dna连接酶活性来检测微生物 Download PDF

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Publication number
CN101849019A
CN101849019A CN200880104327A CN200880104327A CN101849019A CN 101849019 A CN101849019 A CN 101849019A CN 200880104327 A CN200880104327 A CN 200880104327A CN 200880104327 A CN200880104327 A CN 200880104327A CN 101849019 A CN101849019 A CN 101849019A
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CN
China
Prior art keywords
nad
nucleic acid
sample
dependent ligase
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200880104327A
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English (en)
Chinese (zh)
Inventor
克里斯托弗·约翰·斯坦利
斯图尔特·威尔逊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microsens Medtech Ltd
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Microsens Medtech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0713255A external-priority patent/GB0713255D0/en
Priority claimed from GB0719785A external-priority patent/GB0719785D0/en
Priority claimed from GB0802857A external-priority patent/GB0802857D0/en
Priority claimed from GB0807054A external-priority patent/GB0807054D0/en
Application filed by Microsens Medtech Ltd filed Critical Microsens Medtech Ltd
Publication of CN101849019A publication Critical patent/CN101849019A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880104327A 2007-07-09 2008-07-09 基于其nad依赖性dna连接酶活性来检测微生物 Pending CN101849019A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
GB0713255A GB0713255D0 (en) 2007-07-09 2007-07-09 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0713255.8 2007-07-09
GB0713972A GB0713972D0 (en) 2007-07-09 2007-07-18 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0713972.8 2007-07-18
GB0719785.8 2007-10-10
GB0719785A GB0719785D0 (en) 2007-07-09 2007-10-10 Improved detection of micro-organisms
GB0802857.3 2008-02-15
GB0802857A GB0802857D0 (en) 2008-02-15 2008-02-15 Improved bacterial detection methods and kits
GB0807054A GB0807054D0 (en) 2008-04-17 2008-04-17 Improved detection of micro-organisms
GB0807054.2 2008-04-17
PCT/GB2008/002354 WO2009007719A2 (en) 2007-07-09 2008-07-09 Detection of micro-organisms based on their nad-dependent dna ligase activity

Publications (1)

Publication Number Publication Date
CN101849019A true CN101849019A (zh) 2010-09-29

Family

ID=39790901

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880104327A Pending CN101849019A (zh) 2007-07-09 2008-07-09 基于其nad依赖性dna连接酶活性来检测微生物

Country Status (8)

Country Link
US (1) US20100291564A1 (enExample)
EP (1) EP2179052B1 (enExample)
JP (1) JP2010532986A (enExample)
CN (1) CN101849019A (enExample)
AU (1) AU2008273946A1 (enExample)
CA (1) CA2692800A1 (enExample)
MX (1) MX2010000031A (enExample)
WO (1) WO2009007719A2 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0906643D0 (en) * 2009-04-17 2009-06-03 Wilson Stuart M Detection of bacteria and fungi
EP3284831B1 (en) * 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
EP2800822B1 (en) * 2012-01-05 2017-03-29 Zeus Scientific, Inc. Improved dna polymerase activity assays and methods enabling detection of viable microbes
KR20150009539A (ko) * 2012-04-12 2015-01-26 제우스 사이언티픽, 아이엔씨. 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
MX2017013980A (es) * 2015-05-01 2018-06-15 United Animal Health Inc Metodo y composiciones de deteccion y cuantificacion de acido nucleico.
US12157690B2 (en) 2015-12-09 2024-12-03 Microbial Discovery Group Llc Cold temperature-resistant microbials and methods of their use
CN109561712A (zh) 2016-02-29 2019-04-02 微生物发现集团有限责任公司 直接喂饲的微生物
WO2017182775A1 (en) * 2016-04-18 2017-10-26 Momentum Bioscience Limited Microorganism detection involving filtration
US20200354767A1 (en) * 2017-11-16 2020-11-12 The Regents Of The University Of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
US20210115494A1 (en) * 2018-02-07 2021-04-22 Mycometer A/S Improved method for measurement of microbial biomass
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
US12115198B2 (en) 2018-10-02 2024-10-15 ·Microbial Discovery Group, Llc Microbials for animals
CN111118181B (zh) * 2018-10-31 2023-05-12 华东师范大学 一种细菌的检测方法
WO2021035137A1 (en) 2019-08-22 2021-02-25 Microbial Discovery Group, Llc Methods of inhibition with microbial strains and antibiotics
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4931390A (en) * 1986-04-16 1990-06-05 Public Health Research Institute Of The City Of New York, Inc. Expression of the cloned lysostaphin gene
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
KR0148265B1 (ko) 1988-12-16 1998-10-15 에프.지이.엠 헤르만스 자가-지속 서열 복제 시스템
DE4033752A1 (de) * 1990-10-24 1992-04-30 Reissbrodt Rolf Dr Sc Nat Verfahren zur herstellung von lysostaphin
US5976806A (en) * 1997-06-25 1999-11-02 Pioneer Hi-Bred International, Inc. DNA ligase assay
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
EP1224271B1 (en) * 1999-10-19 2009-05-06 Bharat Biotech International, Ltd. Expression of recombinant mature lysostaphin
US6558907B2 (en) * 2001-05-16 2003-05-06 Corning Incorporated Methods and compositions for arraying nucleic acids onto a solid support
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
WO2004106547A2 (en) * 2003-06-02 2004-12-09 Check-Points Bv Fast method for detecting micro-organisms in food samples
GB0324650D0 (en) * 2003-10-22 2003-11-26 Acolyte Biomedica Ltd Using nucleic acids for clinical microbiology testing
WO2005093089A1 (en) * 2004-03-22 2005-10-06 Goldschmidt Gesellschaft Mit Beschränkter Haftung Method and test-kit for the detection and quantification of organisms
US20070009944A1 (en) * 2005-07-01 2007-01-11 University Of East Anglia Assay for nucleic acid ligase and nucleic acid nuclease

Also Published As

Publication number Publication date
WO2009007719A3 (en) 2009-04-09
EP2179052B1 (en) 2012-11-14
EP2179052A2 (en) 2010-04-28
CA2692800A1 (en) 2009-01-15
MX2010000031A (es) 2010-04-21
JP2010532986A (ja) 2010-10-21
US20100291564A1 (en) 2010-11-18
AU2008273946A1 (en) 2009-01-15
WO2009007719A2 (en) 2009-01-15

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Application publication date: 20100929