CA2692800A1 - Detection of micro-organisms based on their nad-dependent dna ligase activity - Google Patents

Detection of micro-organisms based on their nad-dependent dna ligase activity Download PDF

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Publication number
CA2692800A1
CA2692800A1 CA 2692800 CA2692800A CA2692800A1 CA 2692800 A1 CA2692800 A1 CA 2692800A1 CA 2692800 CA2692800 CA 2692800 CA 2692800 A CA2692800 A CA 2692800A CA 2692800 A1 CA2692800 A1 CA 2692800A1
Authority
CA
Canada
Prior art keywords
nad
nucleic acid
sample
dependent ligase
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2692800
Other languages
English (en)
French (fr)
Inventor
Christopher John Stanley
Stuart Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microsens Medtech Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0713255A external-priority patent/GB0713255D0/en
Priority claimed from GB0719785A external-priority patent/GB0719785D0/en
Priority claimed from GB0802857A external-priority patent/GB0802857D0/en
Priority claimed from GB0807054A external-priority patent/GB0807054D0/en
Application filed by Individual filed Critical Individual
Publication of CA2692800A1 publication Critical patent/CA2692800A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2692800 2007-07-09 2008-07-09 Detection of micro-organisms based on their nad-dependent dna ligase activity Abandoned CA2692800A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
GB0713255.8 2007-07-09
GB0713255A GB0713255D0 (en) 2007-07-09 2007-07-09 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0713972.8 2007-07-18
GB0713972A GB0713972D0 (en) 2007-07-09 2007-07-18 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0719785A GB0719785D0 (en) 2007-07-09 2007-10-10 Improved detection of micro-organisms
GB0719785.8 2007-10-10
GB0802857A GB0802857D0 (en) 2008-02-15 2008-02-15 Improved bacterial detection methods and kits
GB0802857.3 2008-02-15
GB0807054.2 2008-04-17
GB0807054A GB0807054D0 (en) 2008-04-17 2008-04-17 Improved detection of micro-organisms
PCT/GB2008/002354 WO2009007719A2 (en) 2007-07-09 2008-07-09 Detection of micro-organisms based on their nad-dependent dna ligase activity

Publications (1)

Publication Number Publication Date
CA2692800A1 true CA2692800A1 (en) 2009-01-15

Family

ID=39790901

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2692800 Abandoned CA2692800A1 (en) 2007-07-09 2008-07-09 Detection of micro-organisms based on their nad-dependent dna ligase activity

Country Status (8)

Country Link
US (1) US20100291564A1 (enExample)
EP (1) EP2179052B1 (enExample)
JP (1) JP2010532986A (enExample)
CN (1) CN101849019A (enExample)
AU (1) AU2008273946A1 (enExample)
CA (1) CA2692800A1 (enExample)
MX (1) MX2010000031A (enExample)
WO (1) WO2009007719A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0906643D0 (en) 2009-04-17 2009-06-03 Wilson Stuart M Detection of bacteria and fungi
NZ701866A (en) 2010-04-16 2016-07-29 Zeus Scientific Inc Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
AU2013206888A1 (en) * 2012-01-05 2014-08-21 Zeus Scientific, Inc. Improved DNA polymerase activity assays and methods enabling detection of viable microbes
KR20150009539A (ko) * 2012-04-12 2015-01-26 제우스 사이언티픽, 아이엔씨. 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US20180298428A1 (en) * 2015-05-01 2018-10-18 Microbial Discovery Group Llc Nucleic acid detection and quantification method and compositions
US12157690B2 (en) 2015-12-09 2024-12-03 Microbial Discovery Group Llc Cold temperature-resistant microbials and methods of their use
WO2017151608A1 (en) 2016-02-29 2017-09-08 Microbial Discovery Group Llc Direct-fed microbials
WO2017182775A1 (en) * 2016-04-18 2017-10-26 Momentum Bioscience Limited Microorganism detection involving filtration
EP3710594B1 (en) * 2017-11-16 2023-07-19 The Regents of The University of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
WO2019154898A1 (en) * 2018-02-07 2019-08-15 Mycometer A/S Improved method for measurement of microbial biomass
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
WO2020072578A1 (en) 2018-10-02 2020-04-09 Microbial Discovery Group, Llc Microbials for animals
CN111118181B (zh) * 2018-10-31 2023-05-12 华东师范大学 一种细菌的检测方法
MX2022002101A (es) 2019-08-22 2022-03-17 Microbial Discovery Group Llc Metodos de inhibicion con cepas microbianas y antibioticos.
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US12514885B2 (en) 2020-08-21 2026-01-06 Microbial Discovery Group, Llc Microbials and antibiotics
BR112023002974A2 (pt) 2020-08-21 2023-04-04 Microbial Discovery Group Llc Cepas microbianas e antibióticos
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4931390A (en) * 1986-04-16 1990-06-05 Public Health Research Institute Of The City Of New York, Inc. Expression of the cloned lysostaphin gene
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
ZA899593B (en) 1988-12-16 1990-09-26 Siska Diagnostics Inc Self-sustained,sequence replication system
DE4033752A1 (de) * 1990-10-24 1992-04-30 Reissbrodt Rolf Dr Sc Nat Verfahren zur herstellung von lysostaphin
US5976806A (en) * 1997-06-25 1999-11-02 Pioneer Hi-Bred International, Inc. DNA ligase assay
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
ATE430799T1 (de) * 1999-10-19 2009-05-15 Bharat Biotech International L Expression von rekombinantem, reifem lysostaphin
US6558907B2 (en) * 2001-05-16 2003-05-06 Corning Incorporated Methods and compositions for arraying nucleic acids onto a solid support
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
WO2004106547A2 (en) * 2003-06-02 2004-12-09 Check-Points Bv Fast method for detecting micro-organisms in food samples
GB0324650D0 (en) * 2003-10-22 2003-11-26 Acolyte Biomedica Ltd Using nucleic acids for clinical microbiology testing
WO2005093089A1 (en) 2004-03-22 2005-10-06 Goldschmidt Gesellschaft Mit Beschränkter Haftung Method and test-kit for the detection and quantification of organisms
US20070009944A1 (en) * 2005-07-01 2007-01-11 University Of East Anglia Assay for nucleic acid ligase and nucleic acid nuclease

Also Published As

Publication number Publication date
US20100291564A1 (en) 2010-11-18
EP2179052A2 (en) 2010-04-28
EP2179052B1 (en) 2012-11-14
WO2009007719A2 (en) 2009-01-15
WO2009007719A3 (en) 2009-04-09
JP2010532986A (ja) 2010-10-21
CN101849019A (zh) 2010-09-29
AU2008273946A1 (en) 2009-01-15
MX2010000031A (es) 2010-04-21

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US20110212438A1 (en) Kits and Devices for Performing Methods of Detecting Viability-Associated Molecules
US9796997B2 (en) Detection of bacteria and fungi
Class et al. Patent application title: Methods for Detection of Micro-Organisms Inventors: Christopher John Stanley (Cambridge, GB) Stuart Wilson (London, GB) Assignees: MICROSEN MEDTECH LIMITED
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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20130628

FZDE Dead

Effective date: 20150709