CN101843298A - Plant composite extract for feed additive, and preparation method and application thereof - Google Patents
Plant composite extract for feed additive, and preparation method and application thereof Download PDFInfo
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- CN101843298A CN101843298A CN201010190998A CN201010190998A CN101843298A CN 101843298 A CN101843298 A CN 101843298A CN 201010190998 A CN201010190998 A CN 201010190998A CN 201010190998 A CN201010190998 A CN 201010190998A CN 101843298 A CN101843298 A CN 101843298A
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a plant composite extract for a feed additive, and a preparation method and application thereof. The plant composite extract is the mixture of a material extract A and a peel extract B of citrus fruits, or the mixture of the extract A, the extract B and rice bran, wherein the material extract A contains tea polysaccharide, tea polyphenol and tea saponin; and the peel extract B contains volatile oil and plant polysaccharide. The plant composite extract has the advantages of natural resource, no residue to the environment, no toxic or side effect on livestock and marine animals and good disease-prevention and growth-promotion effect completely identical with that of a feed antibiotic, and is an ideal substitute for the feed antibiotic.
Description
Technical field
The present invention relates to a kind of plant composite extract for feed additive and preparation method thereof and application, especially relate to plant composite extract for feed additive and preparation method thereof and application that a kind of alternative antibiotic promotes growth of animal.
Background technology
Since 20 middle of century, antibiotic is used in a large number in aquaculture, and its effect of preventing and curing diseases, promote absorption, increase appetite, the promotion of nutrition to grow is generally approved, thereby antibiotic becomes the most widely used feed additive for promoting growth.Yet antibiotic also becomes increasingly conspicuous as the drawback of feed addictive: cause that the pathogen drug resistance strengthens; The consumption of feeding antibiotic constantly increases, and aquaculture cost constantly increases, and medicament residue is more and more serious; Cause the livestock and poultry immunity of organisms to descend; Cause the animal gastrointestinal tract flora imbalance; Antibiotic is residual in a large number in animal products and environment, serious threat human health and Environmental security or the like.European Union has forbidden adding any antibiotic in feed in 2006, strict restriction has also been done to adding antibiotic in the feed by countries such as the Japan and the U.S., China has also constantly strengthened supervision and management to use antibiotic in edible animal, reduces the threat to food security and human health to greatest extent.Completely forbidding the interpolation antibiotic in feed is trend of the times.
Want the total ban feeding antibiotic, reliable measure or effective antibiotic substitute must be arranged, could guarantee animal health and good production performance, and can not produce new safety problem.
Summary of the invention
The objective of the invention is to overcome the defective that existing antibiotics feed addictive exists, provide a kind of and can not produce drug resistance, there is not medicament residue, environmentally safe, feeding antibiotic can be substituted, plant composite extract for feed additive of livestock and poultry and aquatic animal growth and preparation method thereof can be promoted.
Technical scheme of the present invention is:
The present invention's plant composite extract for feed additive is to contain the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin and contain volatile oil and the mixture of the citrus pericarp extract B of plant polyose.
Described contain the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin and contain volatile oil and the mixture of the citrus pericarp extract B of plant polyose in, the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin is the preferred 1:5-9 of 1:1-10(with the weight ratio that contains the citrus pericarp extract B of volatile oil and plant polyose)
The described material that contains tea polysaccharide, Tea Polyphenols and tea saponin is that tealeaves seed, leached tea oil slag, tea seed shell, tealeaves (for reducing cost, can be selected impotable coarse tea leaf for use; Perhaps green tea), two or more compound or in them.
The described citrus pericarp that contains volatile oil and plant polyose is orange skin, fragrant citrus pericarp or shaddock skin, or two or more compound in them.
The present invention's plant composite extract for feed additive, can also be the material extract A that contains tea polysaccharide, Tea Polyphenols and tea saponin, contain the citrus pericarp extract B of volatile oil and plant polyose and rice bran three's mixture, the powdery composite extract that low temperature spray drying obtains; Three's weight proportion is preferably: extract A and extract B add up to weight: rice bran weight=1:0.8-1.2.
The preparation method of the present invention's plant composite extract for feed additive may further comprise the steps:
(1) will contain the drying materials of tea polysaccharide, Tea Polyphenols and tea saponin to water content≤5wt%, be crushed to the 2-5mm fineness again, add the weight of material 3-6 times of 50-90%(volume that is equivalent to contain tea polysaccharide, Tea Polyphenols and tea saponin) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract A concentrate;
(2) the citrus pericarp that will contain volatile oil and plant polyose is dried to water content≤5wt%, be crushed to the 2-5mm fineness again, add and be equivalent to 3-5 times of 50-90%(volume of citrus pericarp weight) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract B concentrate;
(3) extract A concentrate and extract B concentrate are mixed, low temperature spray drying then; After perhaps extract A concentrate and extract B concentrate being mixed, mixture and the rice bran with extract A concentrate and extract B concentrate mixes again, then low temperature spray drying.
The present invention's plant composite extract directly adds in livestock and poultry or aquatic livestock mixed feed, concentrated feed, premix or the complete feed as feed addictive, mixes to get final product.Its addition is advisable to amount to complete feed interpolation 500g-1000g per ton.
The present invention's plant composite extract derives from nature, to the environment noresidue, livestock and poultry, aquatic livestock is had no side effect, but can play and the identical prophyiaxis and promoting growth effect of feeding antibiotic, and be desirable feeding antibiotic substitute.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1: preparation embodiment 1.
(1) takes by weighing coarse tea leaf 10kg, dry, be crushed to the 2-5mm fineness to water content≤5wt%, adding 65%(volume) ethanol water 40L is warming up to 60 ℃, lixiviate 6 hours, filter, filtrate decompression concentrates the concentration that is fit to low temperature spray drying, gets the extract A concentrate;
(2) take by weighing fragrant citrus pericarp 100kg, dry, be crushed to the 2-5mm fineness, use the 65%(volume to water content≤5wt%) ethanol water 300L, be warming up to 60 ℃, lixiviate 5 hours is filtered, remove filter residue, the filtrate normal pressure concentrates the concentration that is fit to low temperature spray drying, gets the extract B concentrate;
(3) extract A concentrate and extract B concentrate are merged together, after mixing, carry out low temperature spray drying, during spray-drying, air themperature is controlled between 110-115 ℃, obtains powdery composite extract product 35kg.
Embodiment 2-4: preparation embodiment 2.-4.
Preparation embodiment 2.-4. raw material variety and quantity and extraction temperature, lixiviate with ethanol water concentration, extract product weight etc., as shown in table 1, surplus with embodiment 1.
Table 1 preparation embodiment raw material variety and quantity and active matter extraction process parameter 2.-4.
Embodiment 5: preparation embodiment 5
(1) takes by weighing coarse tea leaf 9kg, dry, be crushed to the 2-5mm fineness to water content≤5wt%, adding 55%(volume) ethanol water 40L is warming up to 60 ℃, lixiviate 7 hours, filter, filtrate decompression concentrates the concentration that is fit to low temperature spray drying, gets the extract A concentrate;
(2) take by weighing fragrant citrus pericarp 100kg, dry, be crushed to the 2-5mm fineness, use the 75%(volume to water content≤5wt%) ethanol water 350L, be warming up to 60 ℃, lixiviate 5 hours is filtered, remove filter residue, the filtrate normal pressure concentrates the concentration that is fit to low temperature spray drying, gets the extract B concentrate;
(3) extract A concentrate and extract B concentrate are merged together, add rice bran 36kg after mixing again and mix, carry out low temperature spray drying, during spray-drying, air themperature is controlled between 110-115 ℃, obtains powdery composite extract product 72kg.
Embodiment 6: 1. feed weanling pig growth promotion test of Application Example
1 test material and method
1.1 test objective:, check plant composite extract of the present invention in the effect of improving aspect the piglet production performance by contrast test.
1.2 test period: on October 25th, 1 2009 on August 23rd, 2009, totally 63 days.
1.3 experimental animal and grouping: choose 490 of healthy weanling pigs, be divided into two groups at random, every group 12 hurdle, every hurdle piglet 20-21 head of feeding by the close principle of body weight.
1.4 test feed: pig farm autogamy weaning pigs and piggy material, test group feed per ton add plant composite extract 500 grams.
1.5 feeding and management: feeding and management is undertaken by the pig farm routine, and the test weaning pigs of the early stage feeding changes the piggy material into after 25 kilograms.
1.6 experimental observation and record entry: swinery health status, feed intake, weightening finish, feedstuff-meat ratio.
2 test results and analysis
2.1 plant composite extract is to the influence of swinery health status:
The test preliminary trial period,, control group and test group all have the grice diarrhoea situation to occur, and control group is controlled by adding medicine in the feed, and test group is not taked any measure, the pig rehabilitation of suffering from diarrhoea after 3 days of feeding experiment material.
Experimental period control group 34 of disease pigs appear, wherein the disease of digestive tract pig is 10, mainly shows as diarrhea; Other sick pigs mainly show as the respiratory tract infection symptom.12 by the death of failing to respond to any medical treatment.The test group experimental period, do not have pig that disease of digestive tract takes place, but 19 pigs performance respiratory tract infection symptoms, dead 4 of treatment back are arranged.Test group piglet fur is shinny, and mental status is significantly better than control group.
2.2 plant composite extract of the present invention is to the influence of swinery production performance
The table 2 production performance table of comparisons
3. conclusion
By this test, as can be seen, use the plant composite extract of the present invention weanling pig of feeding can draw as drawing a conclusion:
3.1 plant composite extract can obviously improve piglet swinery health status, the disease of digestive tract incidence of disease obviously descends.
3.2 death rate reduces.In this test, survival rate improves 3.3%.
3.3 growth promotion improves daily gain.Daily gain improves 14.2% in this test, and under the suitable substantially situation of initial weight, the average head weightening finish in test end has more 4.06Kg.
3.4 the raising price of deed, remarkable in economical benefits.
Embodiment 7: 2. Application Example substitutes the influence of antibiotic to growth pig production performance and blood parameters
1 materials and methods
1.1 experimental animal and grouping
The FIRE fully-automatic production Performance Measurement System that this test adopts U.S. Ao Siben (Osbern) industrial group to produce; Select 30 of the length that age in days is close, body weight is (31.03 ± 2.13) kg * big healthy galt, by body weight it is divided into 3 processing (each measures the station is a processing) at random, each handles 10 repetitions, and each repeats 1 pig, finishes test when the test pig body weight reaches the 60kg left and right sides.With corn-dregs of beans is basal diet, the kitasamycin (50%) that adds 120mg/kg in basal diet constitutes the antibiotic group, and the plant composite extract that adds 300 (group I), 600mg/kg (group II) on the basal diet basis respectively constitutes 2 plant extracts groups.
1.2 basal diet
With reference to NRC " pig nutritional requirements " (1998) preparation 30～60kg growth pig experimental basis daily ration.The composition and the trophic level of daily ration see Table 3, and feed is powder.
Table 3 basal diet is formed and trophic level (air bells dry basis)
Annotate: premix provides Fe100mg for every kilogram of daily ration; Cu20mg; Zn100mg; Mn4mg; Se0.15mg; I0.14mg; VA5512IU; VD32250IU; VE24mg; VK33mg; VB13mg; VB26mg; VB63mg; VB1224 μ g; D-pantothenic acid 15mg; Nicotinic acid 30.3mg; Folic acid 1.2mg; Biotin 150 μ g.
1.3 feeding and management
Test pig is raised in closed pig house, and comprehensively clean, sterilize measuring the station before the test in the cement flooring, instrument is debugged, calibrated.Each measures station environmental condition unanimity, free choice feeding and drinking-water.Carry out expelling parasite and immunity by the pig farm conventional program.
1.4 testing index
1.4.1 production performance
Production performance is measured with FIRE fully-automatic production Performance Measurement System, and each is measured work station and writes down each every and enter and measure the station data such as the electronic ear board of pig, the time started of searching for food, the concluding time of searching for food, feed consumption and mensuration pig body weight of searching for food.The FIRE system adds up every each feed intake of mensuration pig automatically becomes the feed intake of every day record, and get an effective median the body weight value of measuring from the same day as this mensurations pig body weight on the same day, with this data basis as calculating daily gain (ADG), daily ingestion amount (ADFI) and the price of deed (F/G).
1.4.2 blood parameters
When off-test, 6 pigs of every processing picked at random, with vacuum test tube vena cava anterior blood sampling 6ml, behind the vacuum blood collection blood sampling tube, room temperature has a down dip and places 30min, the centrifugal 10min of 3500r/min, separation of serum is in-20 ℃ of preservations, measure serum urea nitrogen (SUN), serum immunoglobulin IgG, IgM and IgA, total protein (TP), albumin (ALB), glutamic-pyruvic transaminase (GPT), glutamic-oxalacetic transaminease (GOT), alkaline phosphatase (AKP), blood sugar (GLU) with MINDRAYBS-200 automatic clinical chemistry analyzer and kit (available from Shenzhen Mai Rui company); Serum superoxide dismutases (SOD), MDA (MDA), glutathione peroxidase (GSH-Px), serum TAC (TAOC) measure and calculate (kit builds up bio-engineering research institute available from Nanjing) by method in the kit; Adopt the ELISA method to measure serum interleukin 2 (IL-2) and gamma interferon (IFN-γ) content.
1.5 statistical analysis
This test data adopts the SAS9.1 ANOVA of statistical software method to carry out variance analysis, and significant difference then carries out the Deng Kenshi multiple ratio.Data are represented with mean+SD.
2 results and analysis
2.1 plant composite extract is to the influence of growth pig production performance
As shown in Table 4, the ADG that the plant composite extract of 2 levels has all improved the growth pig compares with the antibiotic group, group I, group II have improved 4.9% respectively (P〉0.05), 8.0% (P〉0.05); The ADFI of group I, group II you can well imagine 5.2% (P〉0.05), 6.5% (P〉0.05) high than the antibiotic component; Compare with the antibiotic group, group IF/G has reduced by 4.2% (P〉0.05), and the group II has reduced by 6.1% (P〉0.05).The ADFI of group I, group II all slightly raises than the antibiotic group.
Table 4 growth pig performance test result
Annotate: the different alphabetical persons of colleague's shoulder mark represent significant difference (P＜O.05), do not have shoulder mark or shoulder mark same letter person and represent difference not significantly (P〉O.05).Following table 5-7 herewith.
2.2 serum biochemistry index
As shown in Table 5, group I, group II serum IgG level you can well imagine 8.3% (P〉0.05), 15.20% (P＜0.05) high than antibiotic component; Serum gamma interferon level, group I, group II you can well imagine 4.5% (P〉0.05), 17.3% (P＜0.05) high than the antibiotic component; Total protein, albumin, globulin, white/ball, IgM and interleukin 2 level do not have significant difference between each group; IgA content is lower, fails to detect in this test.
Table 5 growth pig immune indexes measurement result
As shown in Table 6, compare group I, group II serum activity of SOD have improved 8.9% respectively (P〉0.05), 9.9% (P〉0.05) with the antibiotic group; The TAOC vigor has improved 16.5% (P＜0.05), 13.1% (P＜0.05) respectively; MDA content has reduced by 6.3% respectively (P〉0.05), 4.8% (P〉0.05); Each group difference of GSH-Px vigor is not remarkable.
The anti-oxidant index determining result of table 6
As shown in Table 7, group I, group II blood sugar concentration are than antibiotic group (P〉0.05), 6.5% (P〉0.05) that reduced by 5.3% respectively; Blood serum urea nitrogen concentration has reduced by 22.1% (P＜0.05), 24.3% (P＜0.05) respectively; Serum alkaline phosphatase, glutamic-pyruvic transaminase, each group difference of glutamic-oxalacetic transaminease vigor are not remarkable.
Table 7 part serum biochemistry index determining result
3 conclusions
Under this experimental condition, the antibiotic with in the plant composite extract replacement swine rations can draw as drawing a conclusion from above data analysis:
3.1 feed intake, the speed of growth and feed efficiency to pig have the trend that promotes or improve.Under this experimental condition, compare with the antibiotic group, the average daily gain of test group II has improved 8.0%, and test group II feedstuff-meat ratio has reduced by 6.1%.
3.2 can improve the immunologic function of pig, strengthen the oxidation resistance of pig, obviously improve the physique of pig, strengthen resistance against diseases
3.3 the plant composite extract feed addictive has the good health care growth promoting function, can be used as the antibiotic in a kind of green feed additive replacement swine rations, obtains good comprehensive feeding effect.
Embodiment 8: Application Example is 3. to the influence of fattening pig production performance and carcass trait
1 materials and methods
1.1 experimental animal and grouping
The FIRE fully-automatic production Performance Measurement System that this test adopts U.S. Ao Siben (Osbern) industrial group to produce; Select 30 of the length that age in days is close, body weight is (61.80 ± 2.55) kg * big healthy galt, by body weight it is divided into 3 processing (each measures the station is a processing) at random, each handles 10 repetitions, and each repeats 1 pig, finishes test when the test pig body weight reaches the 60kg left and right sides.With corn-dregs of beans is basal diet, and basal diet is a control group, and the plant composite extract of the present invention that adds 200 (group I), 400mg/kg (group II) on the basal diet basis respectively constitutes 2 plant extracts groups.
1.2 basal diet
With reference to NRC " pig nutritional requirements " (1998) preparation 60～100kg growth pig experimental basis daily ration.The composition and the trophic level of daily ration see Table 8, and feed is powder.
Table 8 basal diet is formed and trophic level (air bells dry basis)
Annotate: premix provides Fe100mg for every kilogram of daily ration; Cu5mg; Zn100mg; Mn4mg; Se0.15mg; I0.14mg; VA5512IU; VD32250IU; VE24mg; VK33mg; VB13mg; VB26mg; VB63mg; VB1224 μ g; D-pantothenic acid 15mg; Nicotinic acid 30.3mg; Folic acid; Biotin 150 μ g.
1.3 feeding and management
Test pig is raised in closed pig house, and comprehensively clean, sterilize measuring the station before the test in the cement flooring, instrument is debugged, calibrated.Each measures station environmental condition unanimity, free choice feeding and drinking-water.Carry out expelling parasite and immunity by the pig farm conventional program.
1.4 testing index
1.4.1 production performance
Production performance is measured with FIRE fully-automatic production Performance Measurement System, and each is measured work station and writes down each every and enter and measure the station data such as the electronic ear board of pig, the time started of searching for food, the concluding time of searching for food, feed consumption and mensuration pig body weight of searching for food.The FIRE system adds up every each feed intake of mensuration pig automatically becomes the feed intake of every day record, and get an effective median the body weight value of measuring from the same day as this mensurations pig body weight on the same day, with this data basis as calculating daily gain (ADG), feed intake (ADFI) and the price of deed (F/G).
1.4.2 carcass trait
After the off-test, choose 3 of the heavy test pig of medium body from control group and test group II respectively, fasting is freely drunk water, butcher with reference to boar performance test rules NY/T822-2004 method behind the 24h, measure hot carcass weight, the thickness of backfat, eye muscle area, the tenth ribbed back fat thickness.
The mensuration of muscle pH is measured with reference to the agricultural industry criteria NY/T1333-2007 of the People's Republic of China (PRC) " livestock and poultry meat matter assay method ".
Yellowish pink is got left half trunk the 1st～2 lumbar vertebrae place longissimus dorsi muscle for measuring material, marks with the 5 fens system standard yellowish pink card contrast of pork yellowish pinks under 4 ℃ of conditions in killing back 2h; Marble grain is got chest, longissimus dorsi muscle cross section, lumbar vertebrae junction, and 4 ℃ of preservations contrast marble grain behind the 24h, by 5 fraction systems mark (measuring with reference to the method for Chen Runsheng (1990)).
1.5 statistical analysis
This test data adopts the SAS9.1 ANOVA of statistical software method to carry out variance analysis, and significant difference then carries out the Deng Kenshi multiple ratio.Data are represented with mean+SD.
?
2 results and analysis
2.1 plant composite extract of the present invention is to the influence of fattening pig production performance
As shown in Table 9, the ADG that the plant composite extract of 2 levels has all improved the growth pig compares with control group, and group I has improved 1.6%, and (P〉0.05) group II has improved 12.7% (P＜0.05).Compare with control group, group II F/G has reduced by 2.4% (P〉0.05).The ADFI of group I, group II all has rising than control group.
Table 9 fattening pig performance test result
Annotate: the different alphabetical persons of colleague's shoulder mark represent significant difference (P＜O.05), do not have shoulder mark or shoulder mark same letter person and represent difference not significantly (P〉O.05).Following table together.
2.2 plant composite extract is to the influence of fattening pig carcass trait
As can be seen from Table 10, dressing percentage, lean meat percentage, eye muscle area, the thickness of backfat, yellowish pink, marble grain and muscle PH control group and group II differences all not significantly (P〉0.05).But group II lean meat percentage slightly improves than control group.
Table 10 growing and fattening pigs carcass trait measurement result
3 conclusions
In the fattening pig daily ration, add the 400mg/kg plant composite extract, from above data analysis, can draw as drawing a conclusion:
3.1 can significantly improve the daily gain of pig.Under this experimental condition, the test group II than every pig of antibiotic group 3.44Kg that increases weight more in experimental period.
3.2 to the raising good action of having searched for food.Under this experimental condition, the test group II has improved 11.7% than the average daily ingestion amount of antibiotic group.
3.3 the trend that improves feed efficiency is arranged.Under this experimental condition, feedstuff-meat ratio reduces about 3%.
3.4 meat quality is not had harmful effect, and lean meat percentage slightly improves, and adopts plant composite extract formula feed of the present invention can realize that nonreactive is cultured and acquisition nonreactive safety meat product.
3.5 plant composite extract feed addictive of the present invention has tangible growth promoting function to fattening pig, is the good green feed additive of a kind of comprehensive benefit.
Claims (10)
1. a plant composite extract for feed additive is characterized in that, it is to contain the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin and contain volatile oil and the mixture of the citrus pericarp extract B of plant polyose.
2. plant composite extract for feed additive according to claim 1, it is characterized in that, described contain the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin and contain volatile oil and the mixture of the citrus pericarp extract B of plant polyose in, the material extract A of tea polysaccharide, Tea Polyphenols and tea saponin is 1:1-10 with the weight ratio that contains the citrus pericarp extract B of volatile oil and plant polyose.
3. plant composite extract for feed additive according to claim 1 and 2, it is characterized in that, the described material that contains tea polysaccharide, Tea Polyphenols and tea saponin is tealeaves seed, leached tea oil slag, tea seed shell, coarse tea leaf or green tea, or two or more compound in them.
4. plant composite extract for feed additive according to claim 1 and 2 is characterized in that, the described citrus pericarp that contains volatile oil and plant polyose is orange skin, fragrant citrus pericarp or shaddock skin, or two or more compound in them.
5. plant composite extract for feed additive according to claim 3 is characterized in that, the described citrus pericarp that contains volatile oil and plant polyose is orange skin, fragrant citrus pericarp or shaddock skin, or two or more compound in them.
6. plant composite extract for feed additive, it is characterized in that, it is after the material extract A that contains tea polysaccharide, Tea Polyphenols and tea saponin, the citrus pericarp extract B that contains volatile oil and plant polyose and rice bran mix, the powdery composite extract that low temperature spray drying obtains.
7. the preparation method of plant composite extract for feed additive as claimed in claim 1 or 2 is characterized in that, may further comprise the steps:
(1) will contain the drying materials of tea polysaccharide, Tea Polyphenols and tea saponin to water content≤5wt%, be crushed to the 2-5mm fineness again, add the weight of material 3-6 times of 50-90%(volume that is equivalent to contain tea polysaccharide, Tea Polyphenols and tea saponin) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract A concentrate;
(2) the citrus pericarp that will contain volatile oil and plant polyose is dried to water content≤5wt%, be crushed to the 2-5mm fineness again, add and be equivalent to 3-5 times of 50-90%(volume of citrus pericarp weight) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract B concentrate;
(3) extract A concentrate and extract B concentrate are mixed, low temperature spray drying then.
8. the preparation method as plant composite extract for feed additive as described in the claim 6 is characterized in that, may further comprise the steps:
(1) will contain the drying materials of tea polysaccharide, Tea Polyphenols and tea saponin to water content≤5wt%, be crushed to the 2-5mm fineness again, add the weight of material 3-6 times of 50-90%(volume that is equivalent to contain tea polysaccharide, Tea Polyphenols and tea saponin) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract A concentrate;
(2) the citrus pericarp that will contain volatile oil and plant polyose is dried to water content≤5wt%, be crushed to the 2-5mm fineness again, add and be equivalent to 3-5 times of 50-90%(volume of citrus pericarp weight) ethanol water, be warming up to 50-65 ℃, lixiviate 5-7 hour, then extract is evaporated to the concentration that is fit to low temperature spray drying, gets the extract B concentrate;
(3) extract A concentrate, extract B concentrate and rice bran are mixed, low temperature spray drying then.
9. claim 1 or the 2 described plant composite extract for feed additive application in preparation livestock and poultry, used for aquiculture feed.
10. the application of the described plant composite extract for feed additive of claim 6 in preparation livestock and poultry, used for aquiculture feed.
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| CN103783279A (en) * | 2014-02-21 | 2014-05-14 | 邵阳学院 | Food or feed additive produced by debitterizing orange peel, flavedo and shaddock peel, and preparation method of food or feed additive |
| CN104187194A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Black pony nutritive feed and preparation method thereof |
| CN104187191A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Earthworm feed additive |
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| CN104187191A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Earthworm feed additive |
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| CN104431501A (en) * | 2014-12-01 | 2015-03-25 | 苏州市相城区盛胡特种养殖专业合作社 | Compound feed with activity of inhibiting in-vivo pathogenic bacteria of procambarus clarkii |
| WO2018184522A1 (en) * | 2017-04-03 | 2018-10-11 | 大江生医股份有限公司 | Plant extract composition for use in reducing ultraviolet damage |
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| CN107712300A (en) * | 2017-11-27 | 2018-02-23 | 湖南农业大学 | A kind of health care feedstuff additive for animals and the feed containing the additive |
| CN110833119A (en) * | 2019-10-30 | 2020-02-25 | 李佳璘 | Application of citrus fruit extract in livestock breeding and preparation method thereof |
| CN112262919A (en) * | 2020-10-26 | 2021-01-26 | 南昌师范学院 | Green tea polyphenol chicken feed additive and feed containing same |
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