CN101840652B - Parallel flat flow chamber with asymmetric outlet/inlet - Google Patents
Parallel flat flow chamber with asymmetric outlet/inlet Download PDFInfo
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- CN101840652B CN101840652B CN2010101650386A CN201010165038A CN101840652B CN 101840652 B CN101840652 B CN 101840652B CN 2010101650386 A CN2010101650386 A CN 2010101650386A CN 201010165038 A CN201010165038 A CN 201010165038A CN 101840652 B CN101840652 B CN 101840652B
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Abstract
The invention relates to a parallel flat flow chamber with an asymmetric outlet/inlet in an experimental device for the in-vitro simulation of the flow of blood. The parallel flat flow chamber with the asymmetric outlet/inlet is connected with a peristaltic pump and a liquid storage tank by pipelines to form an integral experimental system to simulate the flow of the blood. Because the constraint conditions of the three-dimensional size of a center rectangular groove of a rectangular base of the parallel flat flow chamber with the asymmetric outlet/inlet are set as follows: the length-to-height ratio is smaller than 360 and greater than 300, and the width-to-height ratio is smaller than 200 and greater than 100, adopting an asymmetric structure of a T type inflow port and a horizontal outflow port and arranging a buffer tank, the height of which is greater than the diameter of the outflow port or the inflow port at the positions of the inflow port and the outflow port respectively, the bottom surface of the flow chamber obtains an effective area with uniform distribution of large-range laminar-flow shearing stress, and the accuracy is obviously enhanced. Furthermore, the invention has simple structure, very simple assembly and maintenance and simple and convenient processing, operation, control and use, can effectively reduce the interference of human factors to a relevant experiment, enhance the accuracy of an experimental result and can be used for researching the regulation and control mechanism of the shearing stress of blood flow to the structure and the function of blood vessels.
Description
One, technical field
The invention belongs to blood vessel biology, biomechanics field, relate to a kind of asymmetric gateway of experimental provision parallel flat flow chamber of in-vitro simulated blood flow.But the present invention's in-vitro simulated blood vessel inner blood flows, and the flow cavity bottom surface can obtain laminar flow shearing stress distribution homogeneous area on a large scale, can be used for studying the regulatory mechanism of blood flow shear stress to blood vessel structure, function.The present invention is easy in processing, operation, control and use than existing similar experimental provision, and marked improvement is more arranged on precision.
Two, background technology
Under the biomechanics environment, tissue growth is grown (growth development) and rebuild (remodeling) is the adaptive change that biological tissue is made environmental change under physiology or the pathological conditions.Human vas spreads all over whole body each tissue and iuntercellular, has the place of blood flow will produce shear stress (shearstress), and shear stress is along blood flow direction vasoactive wall.Blood vessel endothelium has very hypersensitivity to shear stress.Studies show that physiological range shear stress participates in regulating the acute change of blood vessel diameter and these two processes that rely on endothelium of chronic reconstruction of vascular wall.And in diseases such as high fat of blood, hypertension, diabetes and diseases associated with inflammation, the endothelial function damage all can take place.Shear stress yardstick spanning space-time acts on a certain zone, and this zone endothelial cell gene is expressed heterogeneity occurs, be the major reason that causes vascular lesion.Shear stress is regulated blood vessel endothelium physiology and pathological change Study on Mechanism, relate to multidisciplinary fields such as fluid dynamics, biomechanics and cell and molecular biology, the main device that is used for correlative study is parallel flat flow chamber (parallel-plateflow chamber).
At present, many scholars design or improve parallel flat flow chamber as required voluntarily, lack unified standard, various dissimilar parallel flat flow chambers, its basal shear stress distribution situation has nothing in common with each other, with theoretical value of shearing big relative error is arranged, the various instruments that are used for life science all require to have easy to operate, the artificial interference factor is few, characteristics such as precision height, if can not guarantee precision and reduce interference from human factor, this will have a strong impact on the accuracy and the later stage Related product exploitation of experimental result.
Three, summary of the invention
The present invention seeks to by redesigning the asymmetric gateway of a kind of in-vitro simulated blood flow experimental provision parallel flat flow chamber, make the flow cavity bottom surface obtain laminar flow shearing stress distribution homogeneous area on a large scale, thereby it is not high effectively to solve the existing apparatus precision, basal shear stress drawback pockety is for research blood flow shear stress provides more practicable solution to the regulatory mechanism of blood vessel structure, function.
Specifically, asymmetric gateway parallel flat flow chamber has rectangular susceptor, rectangular susceptor central authorities have rectangle groove, the three-dimensional dimension of this rectangle groove satisfies constraint condition: 360＞L/H (length to height ratio)＞300 and 200＞W/H (the ratio of width to height)＞100, respectively there is a Buffer Pool on the rectangle groove both sides, go out to flow the Buffer Pool water receiving head piece of clearing, cover plate has the T type and goes into head piece and rectangular seal groove, place rectangular seal in the rectangular seal groove, cover plate and rectangular susceptor are harmonious the back by the fixing formation of screw hole is whole all around, center rectangle groove sealing formation territory, main flow place; Asymmetric gateway parallel flat flow chamber, peristaltic pump and liquid storage tank connect and compose integral experiment system simulation blood flow by rubber tubing, wherein, following liquid storage tank connects through peristaltic pump goes up liquid storage tank, last liquid storage tank goes out head piece and connects asymmetric gateway parallel flat flow chamber T type and go into head piece, and asymmetric gateway parallel flat flow chamber level goes out head piece and connects down liquid storage tank; Establish run-down pipe in the last liquid storage tank and be connected with following liquid storage tank, unnecessary liquid flows directly into down liquid storage tank through the overflow mouth of pipe, and the liquid storage tank liquid level is constant in the maintenance; Following liquid storage tank is established the air hole of band filter membrane, keeps system's constant pressure; Last liquid storage tank places the metal frame upper mounting plate, and asymmetric gateway parallel flat flow chamber is arranged in the metal frame platform, and following liquid storage tank is positioned at the metal frame lower platform.
The rectangular susceptor center rectangle groove three-dimensional dimension of asymmetric gateway parallel flat flow chamber was satisfying constraint condition 360＞L/H (length to height ratio)＞300 and 200＞W/H (the ratio of width to height)＞100 o'clock, can guarantee that the parallel flat flow chamber basal shear stress of not considering the gateway structure is evenly distributed (with relative error＜2% of middle part, bottom surface value of shearing) effective coverage greater than 85%.
Asymmetric gateway parallel flat flow chamber has that the T type is gone into head piece and level goes out head piece, the T type is gone into head piece and level and is gone out the head piece place and respectively be provided with a height and become a mandarin, go out to flow Buffer Pool greater than what go into (going out) head piece diameter, be used for developing inlet stream, make territory, main flow place produce full-blown Poiseuille flow, guarantee that flow field state is the sheet laminar flow, be used for the simulated blood vessel inner blood and flow; In conjunction with the constraint condition of center rectangle groove three-dimensional dimension, guarantee that basal shear stress is evenly distributed (with relative error＜5% of middle part, bottom surface value of shearing) effective coverage greater than 85%.
Following liquid storage tank is provided with the air hole of band filter membrane, communicates with air, can close at any time by valve, and gas is discharged in convenient experiment beginning back, keeps system's constant pressure, improves system stability effectively, prevents to pollute.
Asymmetric gateway parallel flat flow chamber and liquid storage tank useable glass and plastic production.
The present invention has following marked improvement and advantage:
(1) given the three-dimensional dimension constraint condition of asymmetric gateway parallel flat flow chamber: 360＞L/H (length to height ratio)＞300 and 200＞W/H (the ratio of width to height)＞100, and adopt asymmetrical T type to go into head piece and level goes out the head piece structure, going into head piece and going out the head piece place respectively to be provided with the Buffer Pool of a height, can guarantee that basal shear stress is evenly distributed (with relative error＜5% of middle part, bottom surface value of shearing) effective coverage greater than 85% greater than the head piece diameter of coming in and going out.Efficiently solve the drawback that the parallel flat flow chamber precision is not high, the basal shear stress distribution situation is not good, can be used for studying the regulatory mechanism of blood flow shear stress blood vessel structure, function.
(2) air hole of liquid storage tank tape splicing filter membrane communicates with air under, can close at any time by valve, and convenient experiment beginning back discharge gas keeps system's constant pressure, improves system stability effectively, prevents to pollute.
(3) patent structure of the present invention is simple, and I﹠ M is very simple, has processing, operation, control and advantage easy to use, can reduce the interference of human factor to related experiment effectively, improves the accuracy of experimental result.
Four, description of drawings
Fig. 1 is the asymmetric gateway of the present invention each modular construction synoptic diagram of parallel flat flow chamber.
1 rectangular susceptor, 2 rectangle grooves, 3 Buffer Pools that become a mandarin, 4 go out to flow Buffer Pool, and 5 levels go out head piece, 6 cover plates, the 7T type is gone into head piece, 8 rectangular seal grooves, 9 rectangular seals, 10 screw holes.
Fig. 2 is parallel flat flow chamber cover plate longitudinal profile, the asymmetric gateway of a present invention structural map.
Fig. 3 is parallel flat flow chamber rectangular susceptor longitudinal profile, the asymmetric gateway of a present invention structural map.
Fig. 4 is the experimental system synoptic diagram of the in-vitro simulated blood flow of the present invention.
11 asymmetric gateway parallel flat flow chambers, 12 times liquid storage tanks, 13 peristaltic pumps, liquid storage tank on 14,15 run-down pipes, 16 air holes, 17 valves, 18 metal frame.
Five, embodiment
Below by example the present invention is specifically described, but can not be interpreted as limiting the scope of the invention.
The experimental system that the in-vitro simulated blood vessel inner blood flows contains asymmetric gateway parallel flat flow chamber, peristaltic pump and liquid storage tank shown in Fig. 1～4.Fig. 1～3 are asymmetric gateway parallel flat flow chamber structural representation.Asymmetric gateway parallel flat flow chamber has rectangular susceptor 1, rectangular susceptor central authorities have rectangle groove 2, the three-dimensional dimension of this rectangle groove satisfies constraint condition: 360＞L/H (length to height ratio)＞300 and 200＞W/H (the ratio of width to height)＞100, respectively there is a Buffer Pool 3 on the rectangle groove both sides, 4, go out to flow the Buffer Pool 4 water receivings head piece 5 of clearing, cover plate 6 has the T type and goes into head piece 7 and rectangular seal groove 8, place rectangular seal 9 in the rectangular seal groove, cover plate and rectangular susceptor are harmonious the back by screw hole 10 fixing formations are whole all around, center rectangle groove sealing formation territory, main flow place; Asymmetric gateway parallel flat flow chamber 11, peristaltic pump 13 and liquid storage tank 12,14 connect and compose integral experiment system simulation blood flow by rubber tubing, wherein, following liquid storage tank 12 connects through peristaltic pump 13 goes up liquid storage tank 14, last liquid storage tank goes out head piece and connects asymmetric gateway parallel flat flow chamber 11T type and go into head piece 7, and asymmetric gateway parallel flat flow chamber level goes out head piece 5 and connects down liquid storage tank 12; Establish run-down pipe 15 in the last liquid storage tank and be connected with following liquid storage tank, unnecessary liquid flows directly into down liquid storage tank through the overflow mouth of pipe, and the liquid storage tank liquid level is constant in the maintenance; Following liquid storage tank is established the air hole 16 of band filter membrane, keeps system's constant pressure; Last liquid storage tank places metal frame 18 upper mounting plates, and asymmetric gateway parallel flat flow chamber is arranged in the metal frame platform, and following liquid storage tank is positioned at the metal frame lower platform.
The three-dimensional dimension of the rectangular susceptor center rectangle groove 2 of asymmetric gateway parallel flat flow chamber satisfies constraint condition 360＞L/H (length to height ratio)＞300 and 200＞W/H (the ratio of width to height)＞100, as highly being 0.3mm, length and width are respectively the interior value of scope of 90～108cm and 30～60cm, at this moment, can guarantee that the parallel flat flow chamber basal shear stress of not considering the gateway structure is evenly distributed (with relative error＜2% of middle part, bottom surface value of shearing) effective coverage greater than 85%.
Asymmetric gateway parallel flat flow chamber has the T type and goes into head piece 7 and go out head piece 5 with level, the T type is gone into head piece and level and is gone out the head piece place and respectively be provided with a height and become a mandarin, go out to flow Buffer Pool 3 greater than what go into (going out) head piece diameter, 4, be used for developing inlet stream, make territory, main flow place produce full-blown Poiseuille flow, guarantee that flow field state is the sheet laminar flow, be used for the simulated blood vessel inner blood and flow; In conjunction with the constraint condition of center rectangle groove three-dimensional dimension, guarantee that basal shear stress is evenly distributed (with relative error＜5% of middle part, bottom surface value of shearing) effective coverage greater than 85%.
Following liquid storage tank 12 is provided with the air hole 16 of band filter membrane, communicates with air, can close at any time by valve 17, and gas is discharged in convenient experiment beginning back, keeps system's constant pressure, improves system stability effectively, prevents to pollute.
Asymmetric gateway parallel flat flow chamber and liquid storage tank useable glass and plastic production.
Claims (1)
1. asymmetric gateway parallel flat flow chamber, it is characterized in that: asymmetric gateway parallel flat flow chamber has rectangular susceptor (1), rectangular susceptor central authorities have rectangle groove (2), the three-dimensional dimension of this rectangle groove satisfies constraint condition: 360＞L/H＞300 and 200＞W/H＞100, wherein, L is the length of this rectangle groove, W is the width of this rectangle groove, H is the height of this rectangle groove, the rectangle groove two ends have one to go out to flow Buffer Pool (4) and the Buffer Pool that becomes a mandarin (3) respectively, go out to flow Buffer Pool (4) the water receiving head piece (5) of clearing, cover plate (6) has the T type and goes into head piece (7) and rectangular seal groove (8), place rectangular seal (9) in the rectangular seal groove, the T type is gone into the head piece place and is provided with a height greater than the described Buffer Pool that becomes a mandarin (3) of going into the head piece diameter, level goes out the head piece place and is provided with a height and describedly goes out to flow Buffer Pool (4) greater than what go out the head piece diameter, cover plate and rectangular susceptor are harmonious the back by the fixing formation of screw hole (10) is whole all around, the rectangle groove of rectangular susceptor central authorities (2) sealing formation territory, main flow place; Asymmetric gateway parallel flat flow chamber (11), peristaltic pump (13), following liquid storage tank (12) and last liquid storage tank (14) connect and compose integral experiment system simulation blood flow by rubber tubing, wherein, following liquid storage tank (12) is liquid storage tank (14) in peristaltic pump (13) connection, last liquid storage tank goes out head piece and connects asymmetric gateway parallel flat flow chamber (11) T type and go into head piece (7), and asymmetric gateway parallel flat flow chamber level goes out head piece (5) and connects down liquid storage tank (12); Establish run-down pipe (15) in the last liquid storage tank and be connected with following liquid storage tank, unnecessary liquid flows directly into down liquid storage tank through the overflow mouth of pipe, and the liquid storage tank liquid level is constant in the maintenance; Following liquid storage tank is established the air hole (16) of band filter membrane, keeps system's constant pressure; Last liquid storage tank places metal frame (18) upper mounting plate, and asymmetric gateway parallel flat flow chamber is arranged in the metal frame platform, and following liquid storage tank is positioned at the metal frame lower platform.
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| CN103409312B (en) * | 2013-07-18 | 2014-12-10 | 新疆医科大学 | Cellar mechanical simulation system for researching tumor cell epithelium-mesenchyma conversion |
| CN105176813B (en) * | 2015-09-30 | 2018-04-20 | 重庆大学 | Flow cavity application method is used in a kind of biofluid experiment |
| CN115931700A (en) * | 2020-07-16 | 2023-04-07 | 江苏百赛飞生物科技有限公司 | Flat plate flow cavity experiment system |
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| CN101201348A (en) * | 2007-11-22 | 2008-06-18 | 四川大学 | Mechanics biology experimental installation capable of providing mechanics and chemistry signals synchronously |
| CN101520960A (en) * | 2009-03-31 | 2009-09-02 | 四川大学 | Experimental device for in-vitro simulated blood vessel microenvironment |
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| CN101201348A (en) * | 2007-11-22 | 2008-06-18 | 四川大学 | Mechanics biology experimental installation capable of providing mechanics and chemistry signals synchronously |
| CN101520960A (en) * | 2009-03-31 | 2009-09-02 | 四川大学 | Experimental device for in-vitro simulated blood vessel microenvironment |
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