EA202090534A1 - METHOD FOR PRODUCING MONOCLONAL ANTIBODIES OF THERAPEUTIC PURPOSE USING CONTINUOUS CULTIVATION OF CHO CELLS - Google Patents

METHOD FOR PRODUCING MONOCLONAL ANTIBODIES OF THERAPEUTIC PURPOSE USING CONTINUOUS CULTIVATION OF CHO CELLS

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Publication number
EA202090534A1
EA202090534A1 EA202090534A EA202090534A EA202090534A1 EA 202090534 A1 EA202090534 A1 EA 202090534A1 EA 202090534 A EA202090534 A EA 202090534A EA 202090534 A EA202090534 A EA 202090534A EA 202090534 A1 EA202090534 A1 EA 202090534A1
Authority
EA
Eurasian Patent Office
Prior art keywords
monoclonal antibodies
cho cells
producing monoclonal
therapeutic purpose
antibodies
Prior art date
Application number
EA202090534A
Other languages
Russian (ru)
Other versions
EA037528B1 (en
Inventor
Антон Николаевич МОРОЗОВ
Захар Викторович ЗАХАРОВ
Дмитрий Валерьевич ТЮПА
Роман Александрович КОЧЕЛАБОВ
Original Assignee
Общество С Ограниченной Ответственностью "Международный Биотехнологический Центр "Генериум" (Ооо "Мбц Генериум")
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Общество С Ограниченной Ответственностью "Международный Биотехнологический Центр "Генериум" (Ооо "Мбц Генериум") filed Critical Общество С Ограниченной Ответственностью "Международный Биотехнологический Центр "Генериум" (Ооо "Мбц Генериум")
Publication of EA202090534A1 publication Critical patent/EA202090534A1/en
Publication of EA037528B1 publication Critical patent/EA037528B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к фармацевтической биотехнологии, более конкретно к способам получения терапевтических антител. Предложен способ перфузионного культивирования клеток, позволяющий в промышленных объемах продуцировать, в частности, терапевтические антитела, аффинные к С5-компоненту комплемента, и анти-IgE антитела. К особенностям осуществления способа относятся регулирование скорости протока питательной среды в реакторе, которое позволяет замедлить "старение" клеточной культуры, и регулирование отвода суспензии клеток из биореактора, что позволяет снизить уровень варьирования критических гликанов в полученных антителах.The invention relates to pharmaceutical biotechnology, more specifically to methods for producing therapeutic antibodies. A method of perfusion cell culture is proposed, which makes it possible to produce in industrial volumes, in particular, therapeutic antibodies affinity for the C5 component of complement, and anti-IgE antibodies. The features of the method include regulation of the flow rate of the nutrient medium in the reactor, which allows to slow down the "aging" of the cell culture, and regulation of the withdrawal of the cell suspension from the bioreactor, which makes it possible to reduce the level of variation of critical glycans in the obtained antibodies.

EA202090534A 2017-09-19 2018-09-14 Method of producing monoclonal therapeutic antibodies using continuous culture of cho cells EA037528B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2017132524A RU2672318C1 (en) 2017-09-19 2017-09-19 Method for producing monoclonal antibodies of therapeutic purpose by continuous cultivation of cho cells
PCT/RU2018/000606 WO2019059811A1 (en) 2017-09-19 2018-09-14 Method of producing monoclonal antibodies using continuous culture of cho cells

Publications (2)

Publication Number Publication Date
EA202090534A1 true EA202090534A1 (en) 2020-05-28
EA037528B1 EA037528B1 (en) 2021-04-08

Family

ID=64328023

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202090534A EA037528B1 (en) 2017-09-19 2018-09-14 Method of producing monoclonal therapeutic antibodies using continuous culture of cho cells

Country Status (3)

Country Link
EA (1) EA037528B1 (en)
RU (1) RU2672318C1 (en)
WO (1) WO2019059811A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112592948B (en) * 2020-12-16 2023-05-09 广州汉腾生物科技有限公司 Perfusion culture method of animal cells
AU2023228892A1 (en) * 2022-03-02 2024-08-22 Amgen Inc. Compositions of anti-c5 monoclonal antibody

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA008670B1 (en) * 2003-05-09 2007-06-29 Круселл Холланд Б.В. Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom
US8524496B2 (en) * 2008-09-02 2013-09-03 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
MX369521B (en) * 2013-03-15 2019-11-11 Genzyme Corp High-density cell banking methods.
CN106029896B (en) * 2014-01-13 2020-10-27 美国安进公司 Modulation of ornithine metabolism to manipulate high mannose glycoform content of recombinant proteins

Also Published As

Publication number Publication date
WO2019059811A1 (en) 2019-03-28
EA037528B1 (en) 2021-04-08
RU2672318C1 (en) 2018-11-13

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