CN101810697A - Application of apple polyphenols for preparing liver-protecting medicines, health-care foods or foods - Google Patents
Application of apple polyphenols for preparing liver-protecting medicines, health-care foods or foods Download PDFInfo
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Abstract
The invention discloses an application of apple polyphenols for preparing liver-protecting medicines, health-care foods or foods. The experimental result proves that the apple polyphenols have liver protecting effect, have obvious protecting effect on liver injury models caused by various chemical substances such as CCl4, ethanol, D-GalN, cis-platinum and the like, and have colagogue action; and the AP also has protecting effect on immunological liver injury.
Description
Technical field
The present invention relates to the new purposes of apple polyphenol, particularly relate to apple polyphenol in preparation liver-protecting medicine, health food or Application in Food.
Background technology
Apple polyphenol (AP) is a naturally occurring class important biomolecule active substance in the Fructus Mali pumilae.AP comprises multiple aldehydes matter, can be divided into phenolic acid (as chlorogenic acid) and hydroxy acid esters, carbohydrate derivative and flavone compound (as catechin, epicatechin, procyanidin, dihydroxy chalcone derivative, flavonol glycocide etc.).In general, the kind difference of Fructus Mali pumilae, the content of each main component is also variant.In addition, immature Fructus Mali pumilae is compared with transparent apple, and one-tenth is grouped into similar, but has the content of very big difference, particularly polyphenols to exceed more than 10 times of transparent apple content on the component content.Research finds that also the polyphenol content in the peel is higher than in the sarcocarp far away, and this has also explained and remove the phenomenon that peel can alleviate brown stain in the Fructus Mali pumilae course of processing.
The source of apple polyphenol: for example, Jianfeng Natural Product R﹠D Development Co., Ltd., Tianjin produces.
Main physical and chemical index:: apple polyphenol (UV) 20.0～90.0%, phlorhizin (HPLC) 1.0～20.0%, chlorogenic acid (HPLC) 1.0～30.0%, procyanidin B 2 (HPLC) 1.0～30.0%.
The preparation of AP mainly concentrates on the organic solvent extraction at present, also relevant for adsorbing separation and with the research of supercritical carbon dioxide extraction.
AP has stronger antioxidation, removes the ability of free radical.Its anti-oxidation characteristics embodies by number of ways.AP as hydrogen donor, has scavenging action to various active oxygen with a large amount of phenolic hydroxyl groups, reduces the probability that oxygen-derived free radicals produces, and also is the effective scavengers of various free radicals simultaneously; Secondly AP reduces the catalysis of metal ion to oxidation reaction with ortho position two phenolic hydroxyl groups and metal ion-chelant; Moreover for the system that has oxidase to exist, as the source that oxygen-derived free radicals main in the body generates, AP has significant inhibition ability to it; AP can also and antioxidants such as VC and VE between produce cooperative effect, have the effect of synergist.Result of study proves that many physiologically actives of AP are higher more than 100 times than tea polyphenols, and its oxidation resistance is 50 times of VE, 20 times of VC.
AP based on condensed tannin has resistance of wide spectrum (comprising the inhibition to filamentous fungi, yeast, antibacterial, virus) to microorganism.Its mechanism of action may be the coefficient result of multiple factor, as to proteinic height binding ability, by combining the metabolism that changes microorganism with cell membrane and to the complexing of metal etc.
AP makes it have multiple biological activity to the binding characteristic of biomacromolecule such as protein, polysaccharide and with the chemical property such as complexation characteristic of metal ion, and the most essential aspect of these biological activitys is embodied in the influence of polyphenol to enzymatic reaction.
In addition, AP can also suppress angiotensin transferase (ACE), prevents vasoconstriction, hypertension; AP has inhibitory action to the carcinogenic carcinogenecitys such as benzopyrene in food or environment source; Also has antimutagenic effect; Because Polyphenols has higher ultra-violet absorption feature, particularly energy height, ultra-violet (UV) band that damage capability is big there are stronger absorption, can be used as the effective ingredient of defying age and sunscreen.
Because AP has multiple pharmacology, health care, can develop slow down aging, prophylaxis of tumours, effectively suppress the healthcare products and the plurality kinds of health care food of hypertension, hyperlipidemia and cardiovascular and cerebrovascular disease; AP can effectively suppress the free of histamine, thereby is used as the different drug use of resistance, is used for the prevention and the treatment of diseases such as atopic dermatitis; AP can also be used for toothpaste as additive, existing anti-caries effect has tooth-cleaning action again.Japan's current research is found, makes medicament---the medicinal knit woods PB that promotes natural on-off cycles of hair growth from the extract of Fructus Mali pumilae, and the treatment alopecia is had special-effect, and this discovery has improved the high added value of AP.
Because AP has antioxidation, it is smelly, fresh-keeping to disappear, protect perfume, color fixative, prevent effect such as vitamin loss, at present it can be applicable to aquatic product, poultry meat products, dry sausage goods, bread product, oils and fats or contains the processing and manufacturing of fatty foods, refreshment drink etc. in food industry, can significantly improve its product quality and shelf-life.Also be used for chewing gum and suppress halitosis.
In addition, the effect that AP loses weight in addition and whitens plays multiple action (as defying age, radioprotective, brighten, preserve moisture, hinder ultra-violet absorption etc.) in skin protection, and the skin aging that multiple factor is caused all has unique effect.The radiation-resistant green cosmetics of multiple defying age have been developed at present in the world.
AP is as the native compound of a class biologically active, to people at building body, prevent and cure diseases, each side such as life lengthening all has remarkable effect.
At present, apple polyphenol is not seen bibliographical information in preparation liver-protecting medicine, health food or Application in Food.
This resourceful fruit development of Fructus Mali pumilae awaits further to study, and in the hope of medicine and the health product that exploitation makes new advances, makes it at aspects such as medical treatment, health cares more application be arranged.
Summary of the invention
The object of the present invention is to provide apple polyphenol in preparation liver-protecting medicine, health food or Application in Food.
Technical scheme of the present invention is summarized as follows:
Apple polyphenol is in preparation liver-protecting medicine, health food or Application in Food.
The dosage form of described medicine, health food or food is hard capsule, soft capsule, powder, granule, tablet, pill, sweet unguentum, oral liquid, medicated wine, injection or medicinal tea.
Described food is beverage, confection, rice food, flour product, meat products, fish product or edible oil.
Experimental result proves: apple polyphenol (AP) has liver protection, to the number of chemical material, as CCl
4, liver injury model has significant protective effect due to ethanol, D-GalN and cisplatin (CP) etc., and has choleretic effect, AP also has protective effect to immunologic liver injury.
Description of drawings
Fig. 1 is CCl
4Pathological observation under the acute liver damage model mice hepatic tissue light microscopic.(the pathology picture (* 400) that hepatic tissue HE dyeing light microscopic is observed down: (A) blank group, (B) 0.1%CCl
4Group, (C) BP (bifendate drop pill) 200mg/kg+0.1%CCl
4Group, (D) AP200mg/kg+0.1%CCl
4Group, (E) AP 400mg/kg+0.1%CCl
4Group, (F) 800mg/kg+0.1%CCl
4Group)
Fig. 2 is a pathological observation under the D-GalN acute liver damage model mice hepatic tissue light microscopic.(the pathology picture (* 400) that hepatic tissue HE dyeing light microscopic is observed down: (A) blank group, (B) D-GalN (600mg/kg) group, (C) BP (bifendate drop pill) 200mg/kg+D-GalN (600mg/kg) group, (D) AP 200mg/kg+D-GalN (600mg/kg) group, (E) AP 400mg/kg+D-GalN (600mg/kg) group, (F) AP800mg/kg+D-GalN (600mg/kg) group)
Fig. 3 is a pathological observation under the acute ethanol liver injury model murine liver tissue light microscopic.(the pathology picture (* 400) that hepatic tissue HE dyeing light microscopic is observed down: (A) blank group, (B) ethanol (4.8g/kg) group, (C) BP (bifendate drop pill) 200mg/kg+ ethanol (4.8g/kg) group, (D) AP 200mg/kg+ ethanol (4.8g/kg) group, (E) AP 400mg/kg+ ethanol (4.8g/kg) group, (F) AP 800mg/kg+ ethanol (4.8g/kg) group)
Fig. 4 is a pathological observation under the CP acute liver damage model mice hepatic tissue light microscopic.(the pathology picture (* 400) that hepatic tissue HE dyeing light microscopic is observed down: (A) blank group, (B) CP (3.5mg/kg) group, (C) BP (bifendate drop pill) 200mg/kg+CP (3.5mg/kg) group, (D) AP 200mg/kg+CP (3.5mg/kg) group, (E) AP 400mg/kg+CP (3.5mg/kg) group, (F) AP 800mg/kg+CP (3.5mg/kg) group)
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
The source of apple polyphenol:
Main physical and chemical index:: apple polyphenol (UV, g/100g) 20.0～90.0, phlorhizin (HPLC, g/100g) 1.0～20.0, chlorogenic acid (HPLC, g/100g) 1.0～30.0, procyanidin B 2 (HPLC, g/100g) 1.0～30.0.
Experiment shows that the physical and chemical index of apple polyphenol all has the better protect effect to the hepatic injury cell in following ranges.
| Apple polyphenol (UV, g/100g) | Phlorhizin (HPLC, g/100g) | Chlorogenic acid (HPLC, g/100g) | Procyanidin B 2 (HPLC, g/100g) | |
| Embodiment 1 | ??30 | ??20 | ??30 | ??20 |
| Embodiment 2 | ??20 | ??20 | ??30 | ??30 |
| Embodiment 3 | ??90 | ??5 | ??2 | ??3 |
| Embodiment 4 | ??69 | ??1 | ??15 | ??15 |
| Embodiment 5 | ??88 | ??10 | ??1 | ??1 |
The spontaneous lipid peroxidation of the anti-hepatomicrosome of embodiment 6.AP
The free diet drinking-water of rat adapts to a week, and after water 12h was can't help in fasting, sacrificed by decapitation was got liver rapidly and placed ice-cold normal saline, wash 3 times, and the residual blood in flush away surface, filter paper blots, and weighs.Add normal saline, low-temperature homogenate, (the 0.1g hepatic tissue: behind the homogenate 0.9ml normal saline), the centrifugal 10min of 3000r/min gets supernatant, and it is standby to be stored in refrigerator-80 ℃ refrigerating chamber to make 10%.Measure protein content with the Coomassie brilliant blue method.Face the time spent,, be diluted to the liver homogenate test liquid that protein content is 4mg/ml with normal saline according to protein content.
Experiment is divided into the blank group, and AA organizes (ascorbic acid, positive controls) and AP experimental group.The blank group adds the 1ml distilled water, and all the other each administration groups all add the drug solution 1ml of variable concentrations.Each group adds 1ml liver homogenate, 50 μ l FeSO respectively
4With 20 μ l L-Cys, 30min is hatched in 37 ℃ of water-baths after the dosing, and each group adds 20% acetic acid 2ml, cessation reaction.The centrifugal 15min of 3000r/min gets supernatant 3ml, adds 0.67%TBA 1ml, flowing water cooling behind the boiling water bath 10min.The point plate is used microplate reader and detect its absorbance under 532nm, and calculates clearance rate and IC
50Value.
Clearance rate=(A
The blank group-A
Medicine group to be measured)/A
The blank group* 100%
The result shows, under this experiment condition, when the concentration of AP when 0.014-10mg/ml (AP concentration is all in apple polyphenol, by that analogy herein), to FeSO
4The spontaneous lipid peroxidation of the inductive rat liver microsomes of-L-Cys generates MDA to be had and suppresses effect preferably, and presents certain dose dependent (table 1).Be that AP has the lipid peroxidation that suppresses under the spontaneous oxidation condition, IC
50=0.1063mg/ml.
Table 1 AP is to FeSO
4-L-Cys is inductive
The spontaneous lipid peroxidation of rat liver microsomes generates the inhibitory action of MDA
*P＜0.05,
*P＜0.01,
* *P＜0.001 is compared with the blank group.
Embodiment 7.AP is to 2, bitter diazanyl (2,2-Diphenyl-1-picrylhydrazyl is hereinafter to be referred as the DPPH) measured by esr technique of 2-diphenyl-1-
Get the test sample solution 100 μ l and 1 * 10 of variable concentrations
-4Behind the quick mixing of DPPH alcoholic solution 900 μ l of mol/L respectively at 0,5,10,15,20,25,30,40min...... with microplate reader at 517nm place its absorbance of mensuration, relatively stable until absorbance.Get the absorbance of sample when stablizing, be calculated as follows clearance rate and IC
50Value:
Clearance rate (%)=[Ac-(Ai-Aj)]/Ac * 100%
Annotate: the absorbance of Ac-DPPH solution and sample solvent (distilled water);
The absorbance of Ai-DPPH solution and sample solution;
The absorbance of Aj-DPPH solvent (dehydrated alcohol) and sample solution;
The result shows that after 120min was carried out in reaction, the absorbance of system changed little, thinks that then this system reaches balance.
Table 2 reflection be system reaction in the time of 120 minutes, the AP solution of each concentration is to the clearance rate of DPPH free radical.The concentration that found that AP is 10
-4Show the ability of tangible removing DPPH free radical during-10mg/ml, and be certain dose-dependence.Calculate: AP removes the IC of DPPH free radical
50=2.46 * 10
-3Mg/ml, and the IC of positive drug AA
50=2.26mg/ml.
Table 2 AP is to the influence of DPPH free radical scavenging activity (time=120 minute)
Each numerical value is the meansigma methods of three experiments,
*P＜0.05 is compared with the blank group that only contains DPPH and solvent
Embodiment 8.AP is to CCl
4The protective effect of induced mice acute liver damage cell
60 of mices are divided into 6 groups at random, 10 every group: blank group, CCl
4Group, positive drug group (bifendate drop pill, BP, 200mg/kg), AP administration group (200,400,800mg/kg).BP group and AP administration group gastric infusion every day, the administration volume is 0.2ml/10g, blank and CCl
4The distilled water of group i.g. equal volume every day.Be administered once every day, successive administration 7 days.Behind the last administration 1h, except that the blank group, every group of difference i.p.0.1%CCl
4Soybean oil solution, administration volume 0.1ml/10g, the edible soybean oil of blank group i.p. equal volume.Animal was weighed after water 16h was can't help in fasting, plucked eyeball and got blood, put to death animal, got liver and weighed.The index of surveying has: liver body ratio, and glutamate pyruvate transaminase in the serum (ALT), glutamic oxaloacetic transaminase, GOT (AST) content, and getting the right lobule of liver, to put into 10% formaldehyde fixing, frozen section is cooked conventional H E dyeing, carries out histopathological examination under the light microscopic.
The result shows, compares i.p.0.1%CCl with the blank group
4The liver proportion of mice is obviously increased, and the content of serum alt and AST obviously raises, and the model success is described.AP 200,400 and 800mg/kg all can reduce CCl significantly
4The liver proportion that causes increases, and AP 800mg/kg can also obviously suppress CCl
4The serum alt that causes, the active rising of AST, AP 400mg/kg can significantly suppress the rising of AST, and the influence of ALT is not had significance.The results are shown in Table 3.
Table 3AP is to CCl
4Induced mice acute liver damage liver proportion, Serum ALT, the influence of AST content (mean+/-standard error, n=10)
##P＜0.01, ###P＜0.001 is compared with the blank group;
*P＜0.05,
*P＜0.01,
* *P＜0.001 and CCl
4Group is compared.
Histopathology is observed
1. the perusal of hepatic tissue
The blank group: the liver appearance color is ruddy, smooth surface, the crisp softness of matter.
CCl
4Group: liver volume obviously increases, and color is dark red or be khaki, rough surface, and the liver edge is blunt, and quality is more tough.
AP administration group: liver in color and luster, quality, size and surface flatness than CCl
4Group has clear improvement.
2. the histopathology of hepatic tissue section is observed
The pathology picture is seen Fig. 1.Check result is as follows under the light microscopic:
The blank group: the lobules of liver structural integrity is clear, and hepatic cords is that the center is radial arrangement with the central vein, and hepatic sinusoid is clear, and the hepatocyte form is normal, and endochylema is abundant.
CCl
4Group: the lobules of liver structural fuzzy, show with the central vein to be the hemorrhage and degeneration necrosis at center, how based on spotty necrosis, visible minority strip necrosis.Most of swelling of liver cell in the lobule, Cytoplasm is loose, and the balloon sample that is that has becomes, as seen cavity necrosis widely, spotty necrosis and focal necrosis.The necrotic area is held by the steatosis cell, has in the necrotic area than multi-lymphocytes and inflammatory cell infiltration, and nucleus obviously differs in size, and is pyknosis in various degree.
The AP group: the lobules of liver structure is clear substantially, and the hepatocyte form is more normal, and swelling of liver cell, hyperemia obviously alleviate, and is rare downright bad, a small amount of hepatic cell fattydegeneration, less inflammatory cell infiltration.Along with the increase of AP dosage, hepatocellular protective effect is also strengthened thereupon.
Embodiment 9.AP is to the protective effect of D-GalN induced mice acute liver damage cell
Behind the last administration 1h, except that the blank group, every group of difference i.p.D-GalN 600mg/kg, administration volume 0.2ml/10g, blank group i.p. gives the normal saline of equal volume.Other experimental technique is the same.
The result shows, compares with the blank group, and the active obviously rising of ALT and AST in the D-GalN group mice serum, and the significance variation does not take place in liver proportion, and the modeling success is described.With D-GalN group relatively, AP 800mg/kg can suppress the serum alt that caused by D-GalN significantly and the activity of AST raises; AP 400mg/kg has significance reduction effect to the ALT activity, and the activity of AST in the serum is not had the significance influence; AP200mg/kg does not produce influence significantly to serum alt and AST.The results are shown in Table 4.
Show 4AP to D-GalN induced mice acute liver damage liver proportion, Serum ALT, the influence of AST content (mean+/-standard error, n=10)
###P＜0.001 is compared with the blank group;
*P＜0.05,
*P＜0.01 is compared with the D-GalN group.
Histopathology is observed
1. the perusal of hepatic tissue
The blank group: the liver appearance color is ruddy, smooth surface, the crisp softness of matter.
The D-GalN group: the liver color and luster is darker, and part has obvious white necrosis region.
The AP group: the liver color and luster is darker, still the downright bad point of adularescent.
2. the histopathology of hepatic tissue section is observed
The pathology picture is seen Fig. 2.Check result is as follows under the light microscopic:
The blank group: the lobules of liver structural integrity is clear, and the hepatocyte bundle is that the center is radial arrangement with the central vein, and the portal area does not have inflammatory cell infiltration.
The D-GalN group: pathological changes all appears in hepatic tissue, hepatocyte arrangement disorder in the lobules of liver structural deterioration, lobule, most of swelling of liver cell, the Cytoplasm puffing, sinus hepaticus Kupffer hypertrophy and hepatocellular degeneration, the balloon sample that is that has becomes, as seen the point of cavity necrosis widely, spotty necrosis and the necrosis of kitchen range shape, and inflammatory cell infiltration appears in the portal area, fibrous connective tissue hypertrophy, the tendency that the oriented lobules of liver of outgrowth fibrous tissue extends.
The AP group: the lobules of liver structure exists; the hepatocellular degeneration degree of necrosis is alleviating in various degree; the hepatocyte bundle is radial arrangement; slight granular degeneration; hepatic sinusoid is clear; but still can see single downright bad hepatocyte and acidophilia's degeneration hepatocyte, and portal area local inflammation cellular infiltration, prompting AP has the certain protection effect to D-GalN induced mice acute liver damage cell.
Embodiment 10.AP is to the protective effect of ethanol induced mice acute liver damage cell
Behind the last administration 1h, except that the blank group, every group of difference i.g.50% (v/v) ethanol, administration volume 0.12ml/10g (being equivalent to ethanol 4.8g/kg), blank group i.g. gives the distilled water with equal volume.Other experimental technique is the same.
The result shows, compares with the blank group, and the liver proportion of ethanol model group mice, serum alt and AST content all significantly raise, and illustrate that mice acute ethanol liver injury model duplicates successfully.Compare with the ethanol group, three dosage groups of AP 200,400 and 800mg/kg can rely on the content that ground reduces ALT in the acute ethanol hepatic injury mice serum by dosage, AP 400,800mg/kg can significantly reduce the content of AST in the serum, and have only AP 800mg/kg that the liver proportion increase that ethanol causes is had significance reduction effect.The results are shown in Table 5.
Show 5AP to ethanol induced mice acute liver damage liver proportion, Serum ALT, the influence of AST content (mean+/-standard error, n=10)
##P＜0.01, ###P＜0.001 is compared with the blank group;
*P＜0.05,
*P＜0.01,
* *P＜0.001 is compared with the ethanol group.
Histopathology is observed
1. the perusal of hepatic tissue
After animal gave ethanol, tangible drunk phenomenon appearred, and drowsiness as bradykinesia, excited etc.
The blank group: the liver color is scarlet, smooth surface, the crisp softness of matter.
The ethanol group: volume increases, and the surface is dark red or be khaki, rough surface, and quality is more tough.
The AP group: liver has clear improvement than the ethanol model group in color and luster, quality, size and surface flatness.
2. the histopathology of hepatic tissue section is observed
The pathology picture is seen Fig. 3.Check result is as follows under the light microscopic:
The blank group: the lobules of liver structure is normal, and hepatocyte bar rope queueing discipline is orderly, accidental fat drop, but do not see inflammation and downright bad phenomenon.
The ethanol group: the lobules of liver structure disturbance, hepatocyte moderate steatosis is point-like or coagulation necrosis kitchen range on a small quantity, in the matter inflammatory cell infiltration is arranged.
The AP group: the lobules of liver structure is normal substantially, compares with the ethanol model group, and still visible hepatic cell fattydegeneration of hepatic tissue or ballooning degeneration have slight cloudy swelling phenomenon, but do not see inflammatory cell infiltration and hepatic necrosis.
Embodiment 11.AP is to the protective effect of CP induced mice hepatic injury cell
60 of mices are divided into 6 groups at random, 10 every group: blank group, CP group, BP group (200mg/kg), AP administration group (200,400,800mg/kg).After the administration the 3rd day, when continuing administration, except that the blank group, i.p.CP 3.5mg/kg, continuous 5 days.Medicine and CP dosing interval 6h can fully be absorbed before the CP injection to guarantee medicine.After the CP drug withdrawal, continued the i.g. medicine 2 days (medicine is total to administration 9 days, and CP is total to administration 5 days).The blank group is the distilled water of i.g. equal volume during this period, the normal saline of i.p. equal volume.24h after the last administration gets blood and gets liver, and testing index has: body weight change, liver is heavy, serum alt, AST, histopathologic examination.
The result shows, compares with the blank group, and the amount of losing weight of CP model group mice, serum alt and AST content all significantly raise, and obvious variation does not take place liver proportion, and the success of CP mouse liver injury model copy is described.With the CP model group relatively, AP 800mg/kg can significantly reduce the content of ALT in the CP hepatic injury mice serum, and the content of AST does not have the significance influence in weight loss that CP is caused and the serum; Influence that the content of body weight reduction that AP 200,400mg/kg cause CP and serum alt, AST raises that there are no significant.The results are shown in Table 6.
Show 6AP to cisplatin induced mice acute liver damage liver proportion, Serum ALT, the influence of AST content (mean+/-standard error, n=10)
###P＜0.001 is compared with the blank group;
*P＜0.05,
*P＜0.01,
* *P＜0.001 is compared with the cisplatin group.
Histopathology is observed
1. the perusal of hepatic tissue
The blank group: the liver color is scarlet, smooth surface, the crisp softness of matter.
The CP group: tangible atrophy phenomenon appears in liver, and the surface is dark red.
The AP group: liver is organized the improvement that has to a certain degree in color and luster, quality, size and surface flatness than CP.
2. the histopathology of hepatic tissue section is observed
The pathology picture is seen Fig. 4.Check result is as follows under the light microscopic:
The blank group: the lobules of liver structural integrity is clear, and hepatic cords is that the center is radial arrangement with the central vein, and hepatic sinusoid is clear, and the hepatocyte form is normal, and endochylema is abundant.
The CP group: the lobules of liver structural fuzzy, show with the central vein to be the hemorrhage and degeneration necrosis at center, how based on spotty necrosis, also visible strip necrosis.Most of swelling of liver cell in the lobule, Cytoplasm is loose, and the balloon sample that is that has becomes, as seen cavity necrosis widely, spotty necrosis and focal necrosis.There is more inflammatory cell infiltration the necrotic area, and nucleus obviously differs in size, and is pyknosis in various degree.
AP group: the lobules of liver structure is clear substantially, and swelling of liver cell, hyperemia have and alleviates still visible downright bad, inflammatory cell infiltration phenomenon.
Claims (3)
1. apple polyphenol is in preparation liver-protecting medicine, health food or Application in Food.
2. apple polyphenol according to claim 1 is in preparation liver-protecting medicine, health food or Application in Food, and the dosage form that it is characterized in that described medicine, health food or food is hard capsule, soft capsule, powder, granule, tablet, pill, sweet unguentum, oral liquid, medicated wine, injection or medicinal tea.
3. apple polyphenol according to claim 1 is characterized in that in preparation liver-protecting medicine, health food or Application in Food described food is beverage, confection, rice food, flour product, meat products, fish product or edible oil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103550332A (en) * | 2013-11-15 | 2014-02-05 | 天津市尖峰天然产物研究开发有限公司 | Capsule and preparation containing rich hydroxytyrosol, lycium barbarum polysaccharide and apple polyphenol and used for protecting liver |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060099192A (en) * | 2005-03-10 | 2006-09-19 | (주)바이오뉴트리젠 | A composition for feed additive comprising plant powder or extracts containing antioxidant materials |
| CN1861071A (en) * | 2005-05-12 | 2006-11-15 | 牛蓉 | Medicine and/or health-care products prepd. by using malic polyphenols to regulate immunity |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060099192A (en) * | 2005-03-10 | 2006-09-19 | (주)바이오뉴트리젠 | A composition for feed additive comprising plant powder or extracts containing antioxidant materials |
| CN1861071A (en) * | 2005-05-12 | 2006-11-15 | 牛蓉 | Medicine and/or health-care products prepd. by using malic polyphenols to regulate immunity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103550332A (en) * | 2013-11-15 | 2014-02-05 | 天津市尖峰天然产物研究开发有限公司 | Capsule and preparation containing rich hydroxytyrosol, lycium barbarum polysaccharide and apple polyphenol and used for protecting liver |
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| CN101810697B (en) | 2012-05-23 |
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