CN101810465A - Three-dimensional imaging data acquisition device - Google Patents
Three-dimensional imaging data acquisition device Download PDFInfo
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- CN101810465A CN101810465A CN200910077770A CN200910077770A CN101810465A CN 101810465 A CN101810465 A CN 101810465A CN 200910077770 A CN200910077770 A CN 200910077770A CN 200910077770 A CN200910077770 A CN 200910077770A CN 101810465 A CN101810465 A CN 101810465A
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Abstract
The invention belongs to a three-dimensional imaging data acquisition device, which is composed of a camera and a flat reflecting mirror group, wherein the flat reflecting mirror group comprises four flat reflecting mirrors. By utilizing the flat mirror imaging principle, the surface of the detected object is reflected by the flat reflecting mirrors once or twice to form an image, and the four profiles of the detected object can be acquired after the camera takes two photos of the detected object. Thus, the invention saves the time for taking photos, the three different images are arranged in parallel when taking one photo, the CCD is fully utilized, and the object distance or camera focal distance does not need to change. The design has the advantages of simple structure, convenient operation, low cost and convenient carrying, and can be widely used in the fields of biomedicine, molecular images and the like.
Description
Technical field
The present invention relates to a kind of three-dimensional imaging data acquisition device, exactly, relate to a kind of device that utilizes plane mirror to the object dimensional imaging, this device can be applied to obtain three-dimensional object surface information.
Background technology
Along with developing rapidly of genomics, protein science and disease gene group, the diagnosis of disease is characterizing from traditional disease and is observing, conventional biochemical test detects, develop into the microscopic feature understanding of several genes and molecular level, wherein utilize the molecular image technology can be from generation, the evolution of gene, protein level heightened awareness disease, can realize integral body, continuous, noninvasive special detection method that existing micro analysis institute can't replace, biology will provide brand-new prevention, diagnosis and treatment means in body molecular image theory and technology thereof.With traditional medical image technology comparatively speaking, the basis that molecular imaging is conceived to constitute disease or pathological changes changes and gene molecule level unusual, rather than the final result that is made of the gene molecule change is carried out imaging.Under the help of special molecular probe, the molecular image technology can realize the noinvasive Real-time and Dynamic imaging in vivo of organism internal physiological or pathological process on cell, gene and molecular level, thus for researchs such as the mechanism of action of disease related gene functional localization, cell growth promoter and mutation process, new drug development provide detailed qualitative, location, quantitative data and effectively information obtain means with analyzing and processing.
The autofluorescence tomography technology is the emerging in recent years interior optical molecular image technology of living animal body.Archebiosis fluorescence is with luciferase gene labeled cell or DNA, and the Fluc gene integration is gone up with expressing luciferase to cell chromosome DNA.Under the situation that ATP and oxygen exist, if inject the substrate fluorescein to living animal, the oxidation reaction that luciferase will the catalysis fluorescein also produces photon.External at living animal, utilize highly sensitive optical detecting instrument, can directly capture the outer photon of effusion animal body, utilize effective fluorescence light source algorithm for reconstructing then, just can obtain the position and the intensity of fluorescence light source in the body, and then can observe intravital cell behavior of living animal and gene behavior.
In order as often as possible to obtain overflowing the outer photon information of animal body, need carry out imaging to the three-dimensional surface of animal body with photoelectric detector.At present, the method of utilizing plane mirror to obtain the three-dimensional surface information of animal body has a lot, a kind of method is for using and object plane mirror at 45, successively to the object side surface imaging, this quadrat method has many drawbacks, as: every shooting is once, all to adjust the relative position of primary event mirror and object, so not only the operation trouble is lost time again, the relative position of object changes again easily, to realizing that image Reconstruction provides adverse factors, and the optical information on animal body surface is along with the time decay, and this reconstruction error is just bigger.A kind of method is for using four plane mirrors, be placed on around the object, and become miter angle with object, obtain four profiles of object simultaneously, the shortcoming of this method is that the picture that becomes on CCD of object is little, and reconstruction error is big, and the image of CCD last 3/4 and body surface information are irrelevant, caused insufficient utilization of CCD, the waste resource.A kind of method is taken pictures to around the object simultaneously for using four cameras, increased the expense of equipment like this, and the performance of four cameras is incomplete same, has increased the error of rebuilding.
The four sides information that three-dimensional imaging data acquisition device involved in the present invention just can obtain object by using a camera to take pictures for twice, and three faces of object that obtain of taking pictures for the first time similarly is side by side, the centre does not have unnecessary information, increased the utilization rate of CCD, and take pictures for twice, do not need to change object distance or camera focus, saved and regulated the loaded down with trivial details of camera.
Summary of the invention
The objective of the invention is to overcome the drawback of existing mirror imaging system, provide a kind of and both saved time, can make full use of CCD again, do not need to change the device that object distance or camera focus just can obtain object dimensional body surface information again.
For achieving the above object, the present invention adopts following technical scheme: a kind of three-dimensional imaging data acquisition device includes: plane mirror group and camera, as shown in Figure 1, camera is positioned at the top of plane mirror group.The plane mirror group comprises four plane mirrors, it is characterized in that, the petty action object that body is contained fluorescence light source is put in the position of animal body 5 among Fig. 2, the petty action object is placed perpendicular to paper, twice catoptric imaging of reflecting mirror in top plane mirror and left side passed through in the left side of object in its back, abdominal part passes through twice catoptric imaging of reflecting mirror in following reflecting mirror and left side in the left side of object, with the back picture at grade, its left surface also images in the object left side by the direct reflection of left side reflecting mirror, camera is taken pictures from the top down, once obtain the picture on three surfaces, its right flank picture images in the right side of object by the plane mirror on right side, and camera is taken pictures from the top down and obtained the right flank picture of object.
The invention has the beneficial effects as follows: just can obtain the fluorescence information of four faces of testee by twice shooting, save time, make that the fluorescence decay amplitude is little when taking pictures for twice; The image that once obtains three faces of measured object is form side by side, has made full use of the area of CCD; Do not need to change the focal length of object distance or camera in the process of taking pictures, simplified program, it is simple and convenient to take pictures.Information according to four surfaces of measured object that obtain, utilize the fluorescence light source algorithm for reconstructing, can rebuild accurately the position and the intensity of biological tissue's inside fluorescence light source, be the early diagnosis and the early treatment of disease, that the evaluation of curative effect of medication provides is qualitative accurately, location, quantitative data.
Description of drawings
Fig. 1 is the sketch map of three-dimensional imaging data acquisition device.
Fig. 2 is the schematic diagram of three-dimensional imaging data acquisition device.
Fig. 3 is the sketch map of three surperficial pictures obtaining of once photo taking.
The specific embodiment
By shown in Figure 1, the present invention is made up of camera and plane mirror group, and Fig. 2 is the schematic diagram of three-dimensional imaging data acquisition device.
The present invention is further described below in conjunction with drawings and Examples.The step of utilizing three-dimensional imaging data acquisition device involved in the present invention to experimentize is as follows:
1. at autofluorescence tomography molecule imaging experiment a few days ago, give toy injected fluorescein enzyme; When experiment, to toy injection substrate fluorescein; Biochemical reaction takes place and produces fluorescent photon under the cooperation of ATP and oxygen in luciferase and fluorescein.
2. the toy with anesthesia is placed among Fig. 25 position on all fours, toy back picture at first images in 6 places by plane mirror 3,6 through plane mirrors 1 image in 6 ', the abdominal part picture images in 7 places by plane mirror 4,7 again by plane mirror 1 image in 7 ', toy left surface picture directly by plane mirror 1 image in 5 ', like this back as 6 ', left surface as 5 ' and abdominal part as 7 ' placed side by side, once can photograph three profiles by the liquid nitrogen refrigerating camera from top.The dotted line camera is represented same camera, takes pictures for the second time.Fig. 3 is the sketch map of three profiles obtaining of once photo taking.1 is ccd detector, and 2 is toy back picture, and 3 is toy left surface picture, and 4 is toy abdominal part picture.
3. the movable plane mirror group makes plane mirror position, alignment lens toy right side, and toy right flank picture images in 8 by plane mirror 2, and camera is taken pictures and can be obtained toy right flank picture.
4. utilize the fluorescence light source algorithm for reconstructing, reconstruct the position and the intensity of the intravital fluorescence light source of animal.
Claims (10)
1. three-dimensional imaging data acquisition device includes: camera and plane mirror group, and the plane mirror group is made up of four plane mirrors; It is characterized in that: described testee is placed perpendicular to paper, and four plane mirrors become different angles to place with testee, and taking pictures by twice to obtain four profiles of testee.
2. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that camera is a kind of photodetector.
3. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that two plane mirrors are positioned over the testee left and right sides in four plane mirrors, becomes miter angle with horizontal plane respectively.
4. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that in four plane mirrors that in addition two plane mirrors are positioned over the both sides up and down of testee, become miter angle with vertical plane respectively.
5. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that end face and twice catoptric imaging of underrun of testee, and left side and right side are by the primary event imaging.
6. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that once photo taking wherein can obtain three lateral pictures of difference of testee simultaneously, and three lateral pictures of difference are form side by side.
7. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that imaging is parallel with the ccd detector face.
8. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that in three Polaroid faces, left surface picture and end face, bottom surface picture need the width of the camera depth of field more than or equal to object not on same plane.
9. three-dimensional imaging data acquisition device as claimed in claim 1 is characterized in that camera only needs the position of translated plane mirror group in twice is taken pictures, and does not need to change object distance or camera focus.
10. three-dimensional imaging data acquisition device as claimed in claim 1, when it is characterized in that taking pictures for twice, the optical axis of camera lens will be aimed at the center of left surface or right flank.
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| CN200910077770A CN101810465A (en) | 2009-02-19 | 2009-02-19 | Three-dimensional imaging data acquisition device |
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| CN200910077770A CN101810465A (en) | 2009-02-19 | 2009-02-19 | Three-dimensional imaging data acquisition device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105157601A (en) * | 2015-06-10 | 2015-12-16 | 北京领邦仪器技术有限公司 | Single-camera three-dimensional image measuring instrument |
| CN105873501A (en) * | 2016-03-24 | 2016-08-17 | 中国科学院深圳先进技术研究院 | A fluorescent scattering optical imaging system and method |
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2009
- 2009-02-19 CN CN200910077770A patent/CN101810465A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105157601A (en) * | 2015-06-10 | 2015-12-16 | 北京领邦仪器技术有限公司 | Single-camera three-dimensional image measuring instrument |
| CN105873501A (en) * | 2016-03-24 | 2016-08-17 | 中国科学院深圳先进技术研究院 | A fluorescent scattering optical imaging system and method |
| WO2017161535A1 (en) * | 2016-03-24 | 2017-09-28 | 中国科学院深圳先进技术研究院 | Fluorescent scattering optical imaging system and method |
| CN105873501B (en) * | 2016-03-24 | 2019-03-08 | 中国科学院深圳先进技术研究院 | Fluorescent scattering optical imaging system and method |
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Application publication date: 20100825 |