CN105873501B - Fluorescent scattering optical imaging system and method - Google Patents
Fluorescent scattering optical imaging system and method Download PDFInfo
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- CN105873501B CN105873501B CN201680000101.6A CN201680000101A CN105873501B CN 105873501 B CN105873501 B CN 105873501B CN 201680000101 A CN201680000101 A CN 201680000101A CN 105873501 B CN105873501 B CN 105873501B
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Abstract
It includes: laser, micro-displacement platform, objective table, at least one plane mirror, optical filter, CCD camera and processor that the present invention, which provides a kind of fluorescent scattering optical imaging system and method, the system,；The optical fiber head of the laser is mounted on the micro-displacement platform；The reflecting surface of the plane mirror is towards the determinand on the objective table；The micro-displacement platform and the CCD camera are electrically connected with the processor；Control signal of the micro-displacement platform for managing device according to this is mobile in two-dimensional surface；The laser is used to scan the area to be measured of the determinand of built-in fluorescent material to excite fluorescence；The CCD camera obtains fluorescent image and laser image for direct and reflection based on the plane mirror；The processor generates the three-dimensional fluorescence image in area to be measured for obtaining the location information of CCD camera, the location information of optical fiber head, the location information of plane mirror, the CT image of determinand or MRI image, the fluorescent image and laser image.The present invention can be improved image quality and simplied system structure.
Description
Technical field
The present invention relates to medical imaging technology field more particularly to a kind of fluorescent scattering optical imaging system and methods.
Background technique
Fluorescent scattering optical sectioning imaging technology (Fluorescence Diffuse Optical Tomography,
FDOT working principle) is, in advance in toy et al. Ke tumour and corresponding targeting fluorescent reagent, using laser in toy
Scanning in a certain plane of region, fluorescent reagent stimulated light excitation, emits near infrared light, is swashed later by detector
Luminous picture determines tumour position in animal body and distribution situation finally by three-dimensional reconstruction.With Magnetic resonance imaging
(Magnetic Resonance Imaging, MRI), Computed tomography (Computed Tomography, CT),
Positron emission tomography (PET) (Positron Emission Tomography, PET) is compared, and FDOT imaging has low cost, easily behaviour
The advantages that making, is radiationless is usually used in small animal living body imaging.
It is existing research shows that FDOT combined with PET FDOT/PET bimodal imaging can provide the different life of test articles
Manage function process relevant information.This combination can help to improve the molecular level information provided by given point in time.Compare
Both modalities which imaging system characteristic, PET system rack can acquire signal from each possible angle recordings.But this FDOT/
Structure is complicated for PET bimodal imaging system, and cost is high.
In the FDOT system of existing list angle imaging, CCD (Charge-coupled Device, charge coupled cell) phase
Machine is the top for being fixed on determinand, and excitation light source executes flat scanning in the right opposite of CCD camera, thus existing common
FDOT system is in single angle imaging acquisition structure.Compared with PET, the acquisition mode of the FDOT system of single angle imaging is few, acquisition
Information content is few, and collection period is long, and it is larger to rebuild difficulty.In addition, single angle acquisition image makes FDOT imaging along light source-CCD
In poor quality is imaged in longitudinal tomography in camera line direction.In general, the geometry framework meeting of image capturing system and then shadow
Ring the reconstruction quality of output image.
Summary of the invention
The present invention provides a kind of fluorescent scattering optical imaging system and method, to improve fluorescent scattering optical sectioning imaging matter
Amount shortens imaging time.
The present invention provides a kind of fluorescent scattering optical imaging system, comprising: laser, micro-displacement platform, objective table, at least one
A plane mirror, optical filter, CCD camera and processor；The optical fiber head of the laser is mounted on the micro-displacement platform；Institute
The reflecting surface of plane mirror is stated towards the determinand on the objective table；The micro-displacement platform and the CCD camera respectively with
The processor electrical connection；The micro-displacement platform is used for control signal the setting below the objective table according to the processor
Determine to move in plane domain；The area to be measured that the laser is used to scan the determinand of built-in fluorescent material is glimmering to inspire
Light；The CCD camera is used for the acquisition fluorescent image and laser image from the objective table above, acquisition modes include: directly from
Determinand acquires and the reflection acquisition based on the plane mirror；The processor is used to obtain the position letter of CCD camera
Breath, the CT image of the location information of optical fiber head, the location information of plane mirror, determinand or MRI image, the fluorescent image
And laser image, and take this to generate the three-dimensional fluorescence image in the area to be measured.
In one embodiment, an edge of the plane mirror is fitted on the objective table.
In one embodiment, the system comprises two plane mirrors；Two plane mirrors respectively with
The side of the objective table fitting is parallel to each other, and two plane mirrors are identical as the corner dimension of the objective table.
In one embodiment, the optical filter includes fluorescent optical filter for filtering out fluorescence and for filtering out swashing for laser
Light optical filter；The fluorescent optical filter is 488nm narrow-band pass filter, and the Excitation Filter with High is the long pass filter of 600nm or more
Piece.
The present invention also provides a kind of fluorescent scattering optical imaging methods, comprising: micro-displacement platform is believed according to the control of processor
Number the optical fiber head for carrying laser thereon is driven to move in the setting plane domain below objective table；The laser is treated
The area to be measured for surveying object carries out two dimensional laser scanning to induce the fluorescent material in the area to be measured to issue fluorescence；CCD camera is from institute
Stating acquisition composite fluorescence image and recombination laser image, acquisition mode above objective table includes: directly to be acquired from determinand
And the reflection based on the plane mirror is acquired；Processor obtains the position of the location information of CCD camera, optical fiber head
Information, the location information of plane mirror, the CT image of determinand or MRI image, the composite fluorescence image and recombination laser
Image, and take this to generate the three-dimensional fluorescence image in the area to be measured.
In one embodiment, processor obtains the location information of CCD camera, the location information of optical fiber head, plane mirror
Location information, determinand CT image or MRI image, the composite fluorescence image and recombination laser image, and take this to generate
The three-dimensional fluorescence image in the area to be measured, comprising: be cut into the recombination laser image and composite fluorescence image respectively multiple
Single width laser image and multiple single width fluorescent images；Believed according to the position of the location information of the optical fiber head, the CCD camera
Breath, the location information of the plane mirror, the CT of the determinand or MRI image, the single width laser image and the list
Width fluorescent image passes through the three-dimensional fluorescence image in area to be measured described in three-dimensional reconstruction Software Create.
In one embodiment, this method further include: fluorescent optical filter is set before the CCD camera and filters out the fluorescence
The fluorescence that matter issues；CCD camera acquires recombination laser image above the objective table, comprising: the CCD camera directly acquires
It is emitted by the laser fiber head and is passed through the laser of the determinand, generates first laser image, while acquiring across institute
The laser stating determinand and reflecting through the plane mirror generates second laser image, the first laser image and second
Laser image constitutes the recombination laser image.
In one embodiment, this method further include: Excitation Filter with High is set before the CCD camera and filters out the laser
The laser of optical fiber head outgoing；CCD camera acquires composite fluorescence image above the objective table, comprising: the CCD camera is direct
The fluorescence that acquisition is issued by the fluorescent material in the area to be measured generates the first fluorescent image, while acquiring in the area to be measured
Fluorescent material issue and the reflection through the plane mirror fluorescence, generate the second fluorescent image, first fluorescence
Image and the second fluorescent image constitute the composite fluorescence image.
In one embodiment, according to the location information of the optical fiber head, the location information of the CCD camera, the plane
The location information of reflective mirror, the CT of the determinand or MRI image, the single width laser image and the single width fluorescent image,
Pass through the three-dimensional fluorescence image in area to be measured described in three-dimensional reconstruction Software Create, comprising: software is generated to the CT by volume mesh
Image or MRI image carry out grid dividing, generate the body surface mesh data in the area to be measured；According to the position of the CCD camera
The location information of confidence breath and the plane mirror, is calculated in the plane mirror using mirror-reflection principle
The location information of CCD camera picture；By the location information of the optical fiber head, the location information of the CCD camera, the CCD camera
The location information of picture, the single width laser image, the single width fluorescent image and the body surface mesh data are input to described
In three-dimensional reconstruction software, the three-dimensional fluorescence image is calculated.
In one embodiment, by the location information of the optical fiber head, the location information of the CCD camera, the CCD camera
The location information of picture, the single width laser image, the single width fluorescent image and the body surface mesh data are input to described
In three-dimensional reconstruction software, the three-dimensional fluorescence image is calculated, comprising: scale the laser image and the fluorescent image
It is matched to the actual size in the area to be measured；By the laser image after scaling matching, the fluorogram after scaling matching
The location information and the body of picture, the location information of the location information of the optical fiber head, the CCD camera, the CCD camera picture
Surface mesh data is input in the three-dimensional reconstruction software, and the three-dimensional fluorescence image is calculated.
In one embodiment, the plane mirror is fitted on one side on the objective table；By the recombination laser figure
Picture and composite fluorescence image are cut into multiple single width laser images and multiple single width fluorescent images respectively, comprising: along the plane
The intersection of plane is by the recombination laser image cutting-out at multiple single width where plane where reflective mirror and the objective table
Laser image；Intersection along plane where plane where the plane mirror and the objective table is by the composite fluorescence image
It is cut into multiple single width fluorescent images.
The fluorescent scattering optical imaging system and method for the embodiment of the present invention, by plane mirror reflection laser and glimmering
Light, the laser and fluorescence that can be come out from the angle acquisition for being different from real CCD camera from determinand, to obtain richer
Image reconstruction accuracy can be improved in the two-dimensional fluoroscopic image and two-dimensional laser image information of determinand accordingly, improves reconstruction signal
Intensity, available image quality three-dimensional fluorescence image more higher than existing single angle FDOT system imaging quality.The present invention
The imaging system of embodiment only needs a real CCD camera that can realize multi-angled shooting, the FDOT system with multi-angle imaging
System is compared, and has the advantages that equipment manufacturing cost is low, and real CCD camera and at least one CCD camera picture shoot determinand simultaneously
Laser image and fluorescent image, the image taking speed of the invention at system is faster.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.In the accompanying drawings:
Fig. 1 is the structural schematic diagram of the fluorescent scattering optical imaging system of one embodiment of the invention；
Fig. 2 is the schematic diagram that CCD camera is imaged in plane mirror in one embodiment of the invention；
Fig. 3 is the composite fluorescence image generated of fluorescent scattering optical imaging system shown in Fig. 1 and cuts out schematic diagram；
Fig. 4 is the position setting schematic diagram of plane mirror in one embodiment of the invention；
Fig. 5 is the flow diagram of the fluorescent scattering optical imaging method of one embodiment of the invention；
Fig. 6 is the method flow schematic diagram that three-dimensional fluorescence image is imaged in one embodiment of the invention；
Fig. 7 is the flow diagram that combination picture is cut into the method for single image in one embodiment of the invention；
Fig. 8 is the method flow schematic diagram that three-dimensional fluorescence image is imaged in one embodiment of the invention；
Fig. 9 is the method flow schematic diagram that the imaging of three-dimensional fluorescence image is carried out in one embodiment of the invention.
Specific embodiment
Understand in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, with reference to the accompanying drawing to this hair
Bright embodiment is described in further details.Here, the illustrative embodiments of the present invention and their descriptions are used to explain the present invention, but simultaneously
It is not as a limitation of the invention.
The FDOT system of existing list angle imaging has many advantages, such as low cost, radiationless, but exist acquire mode it is few,
Acquire the disadvantages of information content is few, it is big to rebuild difficulty and longitudinally deteriorates at quality.The advantages of in order to using current FDOT system, invention
People considers influence of the geometry framework of existing FDOT system to its output image quality, by creative labor to existing list
The geometry framework of the FDOT system of angle imaging improves, to improve image quality while guaranteeing image taking speed.
Fig. 1 is the structural schematic diagram of the fluorescent scattering optical imaging system of one embodiment of the invention.As shown in Figure 1, this hair
The fluorescent scattering optical imaging system of bright embodiment can include: laser 101, micro-displacement platform 102, objective table 103, at least one
Plane mirror 104, optical filter 105, CCD camera 106 and processor 107.The optical fiber head of laser 101 is mounted in micro-displacement platform
On 102.The reflecting surface of plane mirror 104 is towards the determinand on objective table 103.Micro-displacement platform 102 and CCD camera 103 are divided
It is not electrically connected with processor 107.
Laser 101 is used to scan the area to be measured of the determinand of built-in fluorescent material to inspire fluorescence.The determinand can
To be living small animal, which can be the tissue or organ of toy, such as tumor region.The laser 101 issues
Laser can be near-infrared laser.As shown in Figure 1, laser 101 emits laser from the bottom to top, the induced with laser objective table
Fluorescent material in determinand on 103 issues fluorescence, and the laser and the fluorescence can be received by CCD camera 106.In other realities
It applies in example, laser 101 can be from determinand be irradiated, correspondingly from top to bottom, and CCD camera 106 can be in the lower section of determinand
Receive laser and fluorescence.
Micro-displacement platform 102 is used for the control signal according to processor 107 in the setting plane domain of 103 lower section of objective table
It is mobile.It is clamped on micro-displacement platform under the drive of micro-displacement platform 102, such as by optical fiber head, the optical fiber head of laser 101 can be with
It is moved in x/y plane along setting path, to carry out two dimensional laser scanning.For example, the position of laser scanning can be it is every along x-axis
Every the mobile position of a set distance, n times are moved altogether, a position is moved every a set distance along y-axis, moves n times altogether,
Form (N+1) * (N+1) array of laser.For another example laser scanning can be using a certain set point as the center of circle, circumferentially
Direction is every the mobile position of a set angle, M times mobile, formation laser array.
Since above-mentioned two kinds of light of laser and fluorescence usually exist simultaneously, so individually acquiring laser image or individually adopting
When collecting fluorescent image, it is acquired again by CCD camera 106 after needing to filter out unwanted light using optical filter 105.Optical filter
105 may include the fluorescent optical filter for filtering out fluorescence and the Excitation Filter with High for filtering out laser.The fluorescent optical filter can be
488nm narrow-band pass filter can only allow CCD camera 106 to collect the light of 488nm, suitable for acquiring the laser of respective wavelength.
The Excitation Filter with High can be the long pass filter piece of 600nm or more, and CCD camera 106 can only be allowed to collect the light of 600nm or more, and
Since under the excitation of 488nm laser, the determinand usually fluorescence of capable of emitting 600~700nm leads to so the 600nm or more is long
Filter plate can preferably acquire fluorescence.
CCD camera 106 is used to obtain fluorescent image above objective table 103 and laser image, acquisition modes include: direct
It is acquired from determinand and the reflection based on plane mirror 104 acquires.Wherein, it is adopted in the way of directly being acquired from determinand
When collecting fluorescent image and laser image, laser and fluorescence are without plane mirror 104, specifically, across the laser of determinand
It is directly entered CCD camera 106, CCD camera 106 is directly entered by the fluorescence that fluorescent material in determinand issues.Two kinds of acquisition sides
The fundamental difference of formula is directly from the laser and fluorescence subsequent propagation come out when determinand acquisition from determinand to CCD camera
106 path is not changed, so the present invention does not limit determinand and CCD camera 106 when directly acquiring image from determinand
Between have other do not change the element of optical path.
In one embodiment, CCD camera 106 can be electron multiplication CCD (Electron-Multiplying CCD,
EMCCD) camera or liquid refrigerating CCD can have more Image Acquisition effect.
Processor 107 is for obtaining the location information of the location information of CCD camera 106, the optical fiber head of laser 101, putting down
The CT image or MRI image, fluorescent image and laser image of determinand on the location information of face reflective mirror 104, objective table 103,
And take this to generate the three-dimensional fluorescence image in above-mentioned area to be measured.Processor 107 can be it is various can be according to the information of above-mentioned input
The equipment that above-mentioned three-dimensional fluorescence image is calculated, such as computer.The location information of plane mirror 104 can be by artificial
It is manually entered into processor 107.It can be calculated using the location information of plane mirror 104 and the location information of CCD camera 106
The location information of CCD camera picture of the CCD camera 106 in plane mirror 104 is obtained, the location information of CCD camera picture is utilized
It can be used for being calculated the three-dimensional fluorescence image in the area to be measured of determinand.The CT image of determinand or MRI figure on objective table 103
3-D image as can be the area to be measured of determinand, can first pass through corresponding equipment in advance and collect.
Fig. 2 is the schematic diagram that CCD camera is imaged in plane mirror in one embodiment of the invention.As shown in Fig. 2, plane
Reflective mirror 104 is used for laser and fluorescent reflection to true CCD camera 106, to form CCD camera as 106a, 106b, and from
Area to be measured is shot as the shooting angle of 106a, 106b different from the CCD camera of the shooting angle of the true CCD camera 106
Two kinds of images of two-dimensional laser image and two-dimensional fluoroscopic image.It should be clear from by Fig. 2, CCD camera 106 is in each plane mirror
It can be at a CCD camera picture in 104.It, can in the fluorescent scattering optical imaging system for being provided with multiple plane mirrors 104
With correspondingly at multiple CCD camera pictures, each CCD camera picture acquires fluorescence from the area to be measured of determinand on objective table 103 and swashs
The angle of light can be different, thus by above-mentioned CCD camera 106 and each CCD camera as available at least from two different angles
Spend the two-dimensional fluoroscopic image and two-dimensional laser image in the area to be measured of shooting.
As depicted in figs. 1 and 2, there are two plane mirrors 104, such construction to be equivalent to three for setting in the system
CCD camera (the CCD camera 106b and the right side virtual on the left of true CCD camera 106 and object at the top of object of same optical properties
The virtual CCD camera 106a in side), while the fluorescence signal issued in object is recorded three positions.With existing multi-angle
FDOT system is compared, through the multi-angle imaging system of plane mirror formation while significantly simplifying system structure, due to
Former single angle system structure is remained, therefore still can be adapted for conventional FDOT image acquisition scheme, it is applied widely.
But the true CCD camera 106 of only one in the fluorescent scattering optical imaging system of the embodiment of the present invention, such one
Come, each two-dimensional fluoroscopic image can be on a width composite fluorescence image, and each two-dimensional laser image can be in a width recombination laser figure
As upper.
Fig. 3 is the composite fluorescence image generated of fluorescent scattering optical imaging system shown in Fig. 1 and cuts out schematic diagram.Such as figure
Shown in 1 and Fig. 3, there are two plane mirrors 104 for setting in the fluorescent scattering optical imaging system of the embodiment, are existed by setting
Two plane mirrors 106 of mouse two sides, an available width include the composite fluorescence image of three width two-dimensional fluoroscopic images
200.The composite fluorescence image 200 includes that the CCD camera 106 at the top of mouse (determinand) takes mouse on objective table 103
CCD camera picture of the fluorescent image 201 and CCD camera 106 at top in two plane mirrors 106 takes mouse respectively
The fluorescent image 202 and 203 of side.
Fig. 4 is the position setting schematic diagram of plane mirror in one embodiment of the invention.As shown in figure 4, plane mirror
104 can be arranged in many ways, can be located at various positions, the angle (such as angle α, β) with horizontal plane can be various
Angle, the distance between plane mirror 104 and determinand L can be various numerical value, as long as plane mirror 104 can be in the future
From the laser in the area to be measured of determinand and fluorescent reflection to CCD camera 106.
In one embodiment, plane mirror 104 be can be set on objective table 103, specifically, can make plane mirror
104 edge fits on objective table 103, in this way, which the setting of plane mirror 104 is convenient, and can collect to be measured
The image of object side.
In one embodiment, which may include two plane mirrors 104.The two planes
The side that reflective mirror 104 is respectively bonded with objective table 103 can be parallel to each other, i.e. two 104 place planes of plane mirror respectively with
The intersection of 103 place plane of objective table can be parallel to each other.The corner dimension of the two plane mirrors 104 and objective table 103 can
It is identical, i.e., in the case where plane mirror 104 is towards above-mentioned determinand, the two 104 place planes of plane mirror and carry
The corner dimension of 103 place plane of object platform can be identical.As shown in Figure 1, the angle α of two plane mirrors 104 and objective table 103
Size with β can be identical, and in other embodiments, the size of angle α and β can be different.Angle α, β size can take to be a variety of
Value, inventor pass through calculating CCD camera 106 and find after the shooting angle of CCD camera picture in plane mirror 104, preferably,
Angle α, β size in 30 °~40 ° ranges, in this way, the image information of available more determinand.
The fluorescent scattering optical imaging system of the embodiment of the present invention can be with by plane mirror reflection laser and fluorescence
The laser and fluorescence come out from the angle acquisition for being different from real CCD camera from determinand, to obtain richer determinand
Two-dimensional fluoroscopic image and two-dimensional laser image information, available image quality is than existing single angle FDOT system imaging accordingly
The higher three-dimensional fluorescence image of quality.It is more that the imaging system of the embodiment of the present invention only needs a real CCD camera that can realize
Angle shot has the advantages that equipment manufacturing cost is low compared with the FDOT system of multi-angle imaging, and real CCD camera and extremely
A few CCD camera picture shoots the laser image and fluorescent image of determinand simultaneously, and the image taking speed of the invention at system is more
Fastly.
Based on inventive concept identical with fluorescent scattering optical imaging system shown in FIG. 1, the embodiment of the present application is also provided
A kind of fluorescent scattering optical imaging method, as described in following example.Since fluorescent scattering optical imaging method solution is asked
The principle of topic is similar to fluorescent scattering optical imaging system, thus the implementation of the fluorescent scattering optical imaging method may refer to it is glimmering
The implementation of light scattering optical imaging system, overlaps will not be repeated.
Fig. 5 is the flow diagram of the fluorescent scattering optical imaging method of one embodiment of the invention.As shown in figure 5, this hair
The fluorescent scattering optical imaging method of bright embodiment, it may include step:
S310: micro-displacement platform drives the optical fiber head for carrying laser thereon in objective table according to the control signal of processor
It is moved in the setting plane domain of lower section；
S320: it is glimmering in above-mentioned area to be measured to induce that above-mentioned laser carries out two dimensional laser scanning to the area to be measured of determinand
Stimulative substance issues fluorescence；
S330:CCD camera acquires composite fluorescence image and recombination laser image, acquisition mode packet above above-mentioned objective table
It includes: being directly acquired from determinand and the reflection based on above-mentioned plane mirror is acquired；
S340: processor obtains the position letter of the location information of CCD camera, the location information of optical fiber head, plane mirror
Breath, the CT image of determinand or MRI image, above-mentioned composite fluorescence image and recombination laser image, and take this to generate above-mentioned to be measured
The three-dimensional fluorescence image in area.
In above-mentioned steps S310, it is clamped on micro-displacement platform under the drive of micro-displacement platform, such as by optical fiber head, laser
The optical fiber head of device can move in setting two-dimensional surface along setting path, to carry out two dimensional laser scanning.For example, institute as above
It states, the position of laser scanning, which can be, moves a position every a set distance along x-axis, n times is moved altogether, along y-axis every one
Set distance moves a position, moves n times altogether, forms (N+1) * (N+1) array of laser.For another example laser scanning can
To be using a certain set point as the center of circle, circumferentially every the mobile position of a set angle, M times mobile, formation swashs
Optical arrays.The laser that the laser issues can be near-infrared laser.
In above-mentioned steps S320, which can be living small animal, which can be the tissue of toy
Or organ, such as tumor region.It is the laser irradiation determinand of 488nm for example, by using wavelength, the fluorescent material in determinand can
To issue the fluorescence of 600~700nm.
In above-mentioned steps S330, composite fluorescence image and recombination laser are acquired in the way of directly acquiring from determinand
When image, laser and fluorescence are reflected without plane mirror, and specifically, the laser across determinand is directly entered CCD camera,
CCD camera is directly entered by the fluorescence that fluorescent material in determinand issues.The fundamental difference of two kinds of acquisition modes be directly from
The laser and fluorescence subsequent propagation to the path of CCD camera come out when determinand acquires from determinand is not changed.Wherein, compound
It may include several two-dimensional laser images in laser image, may include several two-dimensional fluoroscopic images in composite fluorescence image, such as Fig. 3 institute
Show, composite fluorescence image 200 includes three width two-dimensional fluoroscopic images 201,202 and 203.
In above-mentioned steps S340, the CT image or MRI image of the determinand can be the three-dimensional in the area to be measured of determinand
Image can first pass through corresponding equipment in advance and collect.
In the embodiment of the present invention, FDOT imaging system acquired image or can melt with MRI image with CT image co-registration
It closes, to allow function image provided by FDOT during being compared and treated with CT image or MRI image, borrows
It helps the space structure as provided by CT image or MRI image and more intuitive accurately is presented out.
The fluorescent scattering optical imaging system of the embodiment of the present invention passes through plane mirror reflection laser and fluorescence, Ke Yicong
Different from laser and fluorescence that the angle acquisition of real CCD camera comes out from determinand, to obtain richer determinand
Two-dimensional fluoroscopic image and two-dimensional laser image information, available image quality is than existing single angle FDOT system imaging matter accordingly
Measure higher three-dimensional fluorescence image.
Fig. 6 is the method flow schematic diagram that three-dimensional fluorescence image is imaged in one embodiment of the invention.As shown in fig. 6, upper
It states in step S340, processor obtains the position letter of the location information of CCD camera, the location information of optical fiber head, plane mirror
Breath, the CT image of determinand or MRI image, above-mentioned composite fluorescence image and recombination laser image, and take this to generate above-mentioned to be measured
The method of the three-dimensional fluorescence image in area, it may include step:
S341: above-mentioned recombination laser image and composite fluorescence image are cut into multiple single width laser images and multiple respectively
Single width fluorescent image；
S342: according to the location information of above-mentioned optical fiber head, the location information of above-mentioned CCD camera, above-mentioned plane mirror
Location information, the CT of above-mentioned determinand or MRI image, above-mentioned single width laser image and above-mentioned single width fluorescent image, pass through three-dimensional
Reconstruction software generates the three-dimensional fluorescence image in above-mentioned area to be measured.
In above-mentioned steps S341, under the drive of micro-displacement platform, laser is adopted when scanning determinand from different location points
Collect ccd image, and save as the laser image sequence and fluoroscopy image sequence of laser excitation, then by above-mentioned recombination laser image and
When composite fluorescence image is cut into multiple single width laser images and multiple single width fluorescent images respectively, i.e., to above-mentioned laser image
Sequence and fluoroscopy image sequence are cut out processing.Wherein, above-mentioned single width laser image and single width fluorescent image may each be
The image in above-mentioned area to be measured.
In the present embodiment, recombination laser image and composite fluorescence image are cut into multiple single width laser images and more respectively
A single width fluorescent image, can only retain the image in area to be measured, when generating three-dimensional fluorescence image, can only the image for area to be measured into
Row is rebuild, and without rebuilding to non-targeted imaging region, facilitates the reconstruction time for saving three-dimensional fluorescence image, and then improve
Image taking speed.
Fig. 7 is the flow diagram that combination picture is cut into the method for single image in one embodiment of the invention.It is above-mentioned
Plane mirror is fitted on one side on above-mentioned objective table, at this point, as shown in fig. 7, in above-mentioned steps S341, it will be above-mentioned compound
The method that laser image and composite fluorescence image are cut into multiple single width laser images and multiple single width fluorescent images respectively, can wrap
Include step:
S3411: the intersection along plane where plane where above-mentioned plane mirror and above-mentioned objective table will be above-mentioned compound sharp
Light image is cut into multiple above-mentioned single width laser images；
S3412: the intersection along plane where above-mentioned plane mirror and plane where above-mentioned objective table will be above-mentioned compound glimmering
Light image is cut into multiple above-mentioned single width fluorescent images.
In the present embodiment, the intersection along plane where plane mirror and plane where objective table is (compound by combination picture
Laser image, composite fluorescence image) it is cut into multiple single images (single width laser image, single width fluorescent image).One implementation
In example, as shown in figure 3, composite fluorescence image 200 is cut into three width single width fluorescent images 201,202 along intersection 2021,2031
And 203.Wherein, the cut out areas 2022 of single width fluorescent image 202 and the cut out areas 2032 of single width fluorescent image 203 can bases
It needs to select, for example, square region 2022,2032 as shown in Figure 3 can be cut out, then cuts out to obtain the institute, area to be measured of determinand
Corresponding image-region, alternatively, the two-dimensional fluorescent image and laser image in area to be measured are only just cut out by single treatment, tool
Body can be selected optionally.
In the present embodiment, by the way that combination picture is cut along the intersection of plane where plane mirror and plane where objective table
It is cut into single image, complete single image can be readily derived, is less prone to and cuts out fault.
In other embodiments, the recombination laser image and composite fluorescence image cut out using method shown in Fig. 7,
For before generating three-dimensional fluorescence image, which can also to be further cut into the laser in above-mentioned area to be measured
The single width fluorescent image is cut into the fluorescent image in the area to be measured by image, to reduce the data processing amount of processor, improves three
Tie up the imaging time of fluorescent image.
In one embodiment, fluorescent optical filter is set before above-mentioned CCD camera and filters out the fluorescence that above-mentioned fluorescent material issues,
For example, determinand is excited at 488nm, the fluorescence of 600-700nm is issued, the optical filter placed first is the narrowband 488nm
The optical filter of logical (passband 10nm), only allows CCD to collect the light of 488nm.At this point, in above-mentioned steps S330, CCD camera from
The method of acquisition recombination laser image above above-mentioned objective table, it may include step:
S331: above-mentioned CCD camera, which directly acquires, is emitted by above-mentioned laser fiber head and is passed through the laser of above-mentioned determinand,
First laser image is generated, while being acquired across above-mentioned determinand and the laser that is reflected through above-mentioned plane mirror, generates second
Laser image, above-mentioned first laser image and the above-mentioned recombination laser image of second laser image construction.
In one embodiment, Excitation Filter with High is set before above-mentioned CCD camera and filters out above-mentioned laser fiber head outgoing
Laser specifically for example, determinand is excited at 488nm, issues the fluorescence of 600-700nm, change optical filter is
The long logical optical filter of 600nm or more, makes CCD camera collect fluorescent image.At this point, CCD camera is adopted above above-mentioned objective table
Collect the method for composite fluorescence image, it may include step:
S332: above-mentioned CCD camera directly acquires the fluorescence issued by the fluorescent material in above-mentioned area to be measured, and it is glimmering to generate first
Light image, while the sending of the fluorescent material in above-mentioned area to be measured and the reflection through above-mentioned plane mirror fluorescence is acquired, it is raw
At the second fluorescent image, above-mentioned first fluorescent image and the second fluorescent image constitute above-mentioned composite fluorescence image.
Fig. 8 is the method flow schematic diagram that three-dimensional fluorescence image is imaged in one embodiment of the invention.As shown in figure 8, upper
It states in step S342, according to the location information of above-mentioned optical fiber head, the location information of above-mentioned CCD camera, above-mentioned plane mirror
Location information, the CT of above-mentioned determinand or MRI image, above-mentioned single width laser image and above-mentioned single width fluorescent image, pass through three-dimensional
The method that reconstruction software generates the three-dimensional fluorescence image in above-mentioned area to be measured, it may include step:
S3421: generating software by volume mesh and carry out grid dividing to above-mentioned CT image or MRI image, generate it is above-mentioned to
Survey the body surface mesh data in area；
S3422: anti-using mirror surface according to the location information of the location information of above-mentioned CCD camera and above-mentioned plane mirror
Penetrate the location information for the CCD camera picture that principle is calculated in above-mentioned plane mirror；
S3423: by the position of the location information of above-mentioned optical fiber head, the location information of above-mentioned CCD camera, above-mentioned CCD camera picture
Confidence breath, above-mentioned single width laser image, above-mentioned single width fluorescent image and above-mentioned body surface mesh data are input to above-mentioned Three-dimensional Gravity
It builds in software, above-mentioned three-dimensional fluorescence image is calculated.
In above-mentioned steps S3421, volume mesh, which generates software, can be a variety of different grid dividing softwares, such as
Iso2mesh software.In above-mentioned steps S3421, three-dimensional reconstruction software can be a variety of different reconstruction softwares, such as toast soft
Part.
When rebuilding generation three-dimensional fluorescence image using three-dimensional reconstruction software, firstly, utilizing finite element benchmark iterative algorithm
Generate fluorescent image, wherein describe to excite light propagation in area to be measured based on the benchmark iterative algorithm and scatter the coupling of light
Diffusion equation are as follows:
Wherein, r indicates location variable, φxIt is the photon density of exciting light x, φmIt is the photon density for scattering light m,It is the diffusion coefficient of exciting light x,It is diffusion coefficient,
It is the absorption coefficient of exciting light x,It is the absorption coefficient for scattering light m,It is the scattering coefficient of the decaying of exciting light x,It is
The scattering coefficient of the decaying of light m is scattered, a is the boundary related coefficient of internal reflection, Sx(r)=S0δ(r-r0) it is exciting light x point
The excitation source item in source, S0Indicate the intensity of point source, δ (r-r0) it is with position r0Point source centered on Dirac-delta function, η
It is edge-diffusion coefficient,For the absorption coefficient of exciting lightOr the absorption coefficient of scattering light
Shown in the equation matrix such as formula (1) obtained using finite element discretization relationship and (2), a series of use are further obtained
In the equation for solving inverse problem:
[Ax,m]{φx,m}={ bx,m, (3)
Wherein, matrix [Ax,m] parameter and column vector { bx,mIn item can indicate that glug is bright with one group of spatial variations
Day basic function；Jx,mIt is by φx,mDerivative each boundary observe node for χ Jacobian matrix；Δ χ be optics and
Fluorescent characteristic distributed update vector；I is unit matrix；λ can be a scale or diagonal matrix；It is matrix Jx,mTransposition
Matrix；χ is fluorescent characteristic distributing vector, expresses Dx、Orφx,mBe exciting light x or scatter light m photon it is close
Degree；It is the exciting light x being observed or the photon density for scattering light m；It is the exciting light x being calculated or scattering light m
Photon density.Laser image and fluorescent image pass through formula (3)~(5) and iteratively solve formation, and probably equal from these attributes
Even preresearch estimates updates optical fluorescence characteristic distribution.
Fig. 9 is the method flow schematic diagram that the imaging of three-dimensional fluorescence image is carried out in one embodiment of the invention.As shown in figure 9,
In above-mentioned steps S3423, by the location information of above-mentioned optical fiber head, the location information of above-mentioned CCD camera, above-mentioned CCD camera picture
Location information, above-mentioned single width laser image, above-mentioned single width fluorescent image and above-mentioned body surface mesh data be input to above-mentioned three
It ties up in reconstruction software, the method that above-mentioned three-dimensional fluorescence image is calculated, it may include step:
S34231: above-mentioned laser image and above-mentioned fluorescent image scaling are matched to the actual size in above-mentioned area to be measured；
S34232: by the above-mentioned laser image after scaling matching, the above-mentioned fluorescent image after scaling matching, above-mentioned optical fiber head
Location information, the location information of above-mentioned CCD camera, the location information of above-mentioned CCD camera picture and above-mentioned body surface mesh data
It is input in above-mentioned three-dimensional reconstruction software, above-mentioned three-dimensional fluorescence image is calculated.
In one embodiment, fluorescent scattering optical imaging method comprising steps of
1) object that need to be reconstructed is placed on objective table, object is interior glimmering containing that can inspire under corresponding excitation light source
The fluorescent material of light, the visual field for adjusting CCD camera can extremely cover whole object；
2) upper filter is transferred in CCD camera, filters the fluorescence of object sending.Such as object under 488nm laser by
Excitation issues the fluorescence that wavelength is 600-700nm.The optical filter placed first is the optical filtering of 488nm narrow bandpass (passband 10nm)
Piece only allows CCD to collect the light of 488nm.
3) two-dimensional micro-displacement platform is operated, is allowed to mobile by the program finished in processor, that is, moves the position of laser fiber head
It sets, is allowed to emit laser in different positions；
4) ccd image is acquired on laser different scanning position simultaneously, saves as the image sequence of laser excitation；
5) optical filter is changed, such as object is excited under 488nm laser, it, can be more when issuing the fluorescence of 600-700nm
Changing optical filter is the long logical optical filter of 600nm or more, and CCD is made to collect fluorescent image；
6) the recombination laser image and composite fluorescence image photographed to CCD camera is cut out, the object in front and two sides
Body image cutting-out is opened, and can be cut out using the intersection of plane mirror and horizontal plane as cut-off rule.
7) the CT image of object is scanned, and generates the surface body of object by the software of generation volume mesh (such as iso2mesh)
Grid data grid mesh；
8) laser image and fluorescent image and CCD camera, (true CCD camera and CCD camera are in plane mirror
In picture) with the location information of laser source, input information of the mesh information of object as reconstruction software is soft by three-dimensional reconstruction
Part (such as toast) generates the three-dimensional data comprising fluorescence distribution.
In step 8), which includes the positive fluorescent image for the object that segmentation obtains and the fluorogram of side
Picture.Specific Cut out step can include: some region of interest ROI region of selected object, such as mouse lung (such as having a size of
1.2cm*1.2cm), laser image, the fluorescent image after selected ROI region are all matched with the actual size of object, so
After be cut out ROI region, pass through three-dimensional reconstruction Software Create Jacobian matrix.Location information includes: the location information of CCD
(such as CCD camera location information is " 12 14 40 0 0-1 ", successively indicates the coordinate of x-axis, y-axis, z-axis, unit mm, 0
0-1 indicates that CCD is to acquire downwards), (such as laser source location information is such as " 12 14-5 00 for the location information of laser source
1 ", successively indicate that the coordinate of x-axis, y-axis, z-axis, unit mm, 001 expression laser sources excite upwards), mesh information (i.e. body
Surface mesh three-dimensional coordinate, for example, 25.595,60.6565,20.565, respectively indicate the coordinate of x-axis, y-axis, z-axis), pass through
Three-dimensional reconstruction software (such as call toastMapSolToMesh, toastSolutionMask, IWT2_P0, FDOTAdj0p or
TostQvec etc.) Lai Chongjian obtains distributed intelligence of the fluorescent material in object, i.e. three-dimensional fluorescence image.
The fluorescent scattering optical imaging method of the embodiment of the present invention can be with by plane mirror reflection laser and fluorescence
The laser and fluorescence come out from the angle acquisition for being different from real CCD camera from determinand, to obtain richer determinand
Two-dimensional fluoroscopic image and two-dimensional laser image information, image reconstruction accuracy can be improved accordingly, improve the intensity of reconstruction signal,
Available image quality three-dimensional fluorescence image more higher than existing list angle FDOT system imaging quality.The embodiment of the present invention
Imaging system only needs a real CCD camera that can realize multi-angled shooting, compared with the FDOT system of multi-angle imaging, tool
Have the advantages that equipment manufacturing cost is low, and real CCD camera and at least one CCD camera picture shoot the laser image of determinand simultaneously
And fluorescent image, the image taking speed of the invention at system is faster.
In the description of this specification, reference term " one embodiment ", " specific embodiment ", " some implementations
Example ", " such as ", the description of " example ", " specific example " or " some examples " etc. mean it is described in conjunction with this embodiment or example
Particular features, structures, materials, or characteristics are included at least one embodiment or example of the invention.In the present specification,
Schematic expression of the above terms may not refer to the same embodiment or example.Moreover, the specific features of description, knot
Structure, material or feature can be combined in any suitable manner in any one or more of the embodiments or examples.
It should be understood by those skilled in the art that, the embodiment of the present invention can provide as method, system or computer program
Product.Therefore, complete hardware embodiment, complete software embodiment or reality combining software and hardware aspects can be used in the present invention
Apply the form of example.Moreover, it wherein includes the computer of computer usable program code that the present invention, which can be used in one or more,
The computer program implemented in usable storage medium (including but not limited to magnetic disk storage, CD-ROM, optical memory etc.) produces
The form of product.
The present invention be referring to according to the method for the embodiment of the present invention, the process of equipment (system) and computer program product
Figure and/or block diagram describe.It should be understood that every one stream in flowchart and/or the block diagram can be realized by computer program instructions
The combination of process and/or box in journey and/or box and flowchart and/or the block diagram.It can provide these computer programs
Instruct the processor of general purpose computer, special purpose computer, Embedded Processor or other programmable data processing devices to produce
A raw machine, so that being generated by the instruction that computer or the processor of other programmable data processing devices execute for real
The device for the function of being specified in present one or more flows of the flowchart and/or one or more blocks of the block diagram.
These computer program instructions, which may also be stored in, is able to guide computer or other programmable data processing devices with spy
Determine in the computer-readable memory that mode works, so that it includes referring to that instruction stored in the computer readable memory, which generates,
Enable the manufacture of device, the command device realize in one box of one or more flows of the flowchart and/or block diagram or
The function of being specified in multiple boxes.
These computer program instructions also can be loaded onto a computer or other programmable data processing device, so that counting
Series of operation steps are executed on calculation machine or other programmable devices to generate computer implemented processing, thus in computer or
The instruction executed on other programmable devices is provided for realizing in one or more flows of the flowchart and/or block diagram one
The step of function of being specified in a box or multiple boxes.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical scheme and beneficial effects
Describe in detail it is bright, it should be understood that the above is only a specific embodiment of the present invention, the guarantor being not intended to limit the present invention
Range is protected, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this
Within the protection scope of invention.
Claims (9)
1. a kind of fluorescent scattering optical imaging system characterized by comprising laser, micro-displacement platform, objective table, at least one
A plane mirror, optical filter, CCD camera and processor；
The optical fiber head of the laser is mounted on the micro-displacement platform；The reflecting surface of the plane mirror is towards the loading
Determinand on platform；The micro-displacement platform and the CCD camera are electrically connected with the processor respectively；
The micro-displacement platform is used in the setting plane domain according to the control signal of the processor below the objective table
It is mobile；
The laser is used to scan the area to be measured of the determinand of built-in fluorescent material to inspire fluorescence；
The CCD camera is used for the acquisition fluorescent image and laser image from the objective table above, acquisition modes include: directly from
Determinand acquires and the reflection acquisition based on the plane mirror；
The processor is used to obtain the position letter of the location information of CCD camera, the location information of optical fiber head, plane mirror
Breath, the CT image of determinand or MRI image, the fluorescent image and laser image, and take this to generate the three-dimensional in the area to be measured
Fluorescent image；
Fluorescent scattering optical imaging method is carried out using the fluorescent scattering optical imaging system, comprising:
Micro-displacement platform drives optical fiber head the setting below objective table for carrying laser thereon according to the control signal of processor
Determine to move in plane domain；
The laser carries out two dimensional laser scanning to the area to be measured of determinand to induce the fluorescent material in the area to be measured to send out
Fluorescence out；
CCD camera acquires composite fluorescence image and recombination laser image above the objective table, acquisition mode include: directly from
Determinand is acquired and the reflection based on the plane mirror is acquired；
Processor obtains location information, the determinand of the location information of CCD camera, the location information of optical fiber head, plane mirror
CT image or MRI image, the composite fluorescence image and recombination laser image, and the three-dimensional for taking this to generate the area to be measured is glimmering
Light image；
Processor obtains location information, the determinand of the location information of CCD camera, the location information of optical fiber head, plane mirror
CT image or MRI image, the composite fluorescence image and recombination laser image, and the three-dimensional for taking this to generate the area to be measured is glimmering
Light image, comprising:
The recombination laser image and composite fluorescence image are cut into multiple single width laser images and multiple single width fluorescence respectively
Image；
According to the location information of the optical fiber head, the location information of the CCD camera, the plane mirror location information,
The CT or MRI image of the determinand, the single width laser image and the single width fluorescent image, it is raw by three-dimensional reconstruction software
At the three-dimensional fluorescence image in the area to be measured；
According to the location information of the optical fiber head, the location information of the CCD camera, the plane mirror location information,
The CT or MRI image of the determinand, the single width laser image and the single width fluorescent image, it is raw by three-dimensional reconstruction software
At the three-dimensional fluorescence image in the area to be measured, comprising:
Software is generated by volume mesh, grid dividing is carried out to the CT image or MRI image, generate the body surface in the area to be measured
Surface grids data；
According to the location information of the location information of the CCD camera and the plane mirror, calculated using mirror-reflection principle
Obtain the location information of the CCD camera picture in the plane mirror；
By the location information of the optical fiber head, the location information of the CCD camera, the CCD camera picture location information, described
Single width laser image, the single width fluorescent image and the body surface mesh data are input in the three-dimensional reconstruction software, meter
Calculation obtains the three-dimensional fluorescence image.
2. fluorescent scattering optical imaging system as described in claim 1, which is characterized in that an edge of the plane mirror
It fits on the objective table.
3. fluorescent scattering optical imaging system as claimed in claim 2, which is characterized in that described flat the system comprises two
Face reflective mirror；The side that two plane mirrors are respectively bonded with the objective table is parallel to each other, and two planes are anti-
Light microscopic is identical as the corner dimension of the objective table.
4. fluorescent scattering optical imaging system as described in claim 1, which is characterized in that the optical filter includes for filtering out
The fluorescent optical filter of fluorescence and Excitation Filter with High for filtering out laser；The fluorescent optical filter is 488nm narrow-band pass filter,
The Excitation Filter with High is the long pass filter piece of 600nm or more.
5. a kind of fluorescent scattering optical imaging method characterized by comprising
Micro-displacement platform drives optical fiber head the setting below objective table for carrying laser thereon according to the control signal of processor
Determine to move in plane domain；
The laser carries out two dimensional laser scanning to the area to be measured of determinand to induce the fluorescent material in the area to be measured to send out
Fluorescence out；
CCD camera acquires composite fluorescence image and recombination laser image above the objective table, acquisition mode include: directly from
Determinand is acquired and the reflection based on the plane mirror is acquired；
Processor obtains location information, the determinand of the location information of CCD camera, the location information of optical fiber head, plane mirror
CT image or MRI image, the composite fluorescence image and recombination laser image, and the three-dimensional for taking this to generate the area to be measured is glimmering
Light image；
Processor obtains location information, the determinand of the location information of CCD camera, the location information of optical fiber head, plane mirror
CT image or MRI image, the composite fluorescence image and recombination laser image, and the three-dimensional for taking this to generate the area to be measured is glimmering
Light image, comprising:
The recombination laser image and composite fluorescence image are cut into multiple single width laser images and multiple single width fluorescence respectively
Image；
According to the location information of the optical fiber head, the location information of the CCD camera, the plane mirror location information,
The CT or MRI image of the determinand, the single width laser image and the single width fluorescent image, it is raw by three-dimensional reconstruction software
At the three-dimensional fluorescence image in the area to be measured；
According to the location information of the optical fiber head, the location information of the CCD camera, the plane mirror location information,
The CT or MRI image of the determinand, the single width laser image and the single width fluorescent image, it is raw by three-dimensional reconstruction software
At the three-dimensional fluorescence image in the area to be measured, comprising:
Software is generated by volume mesh, grid dividing is carried out to the CT image or MRI image, generate the body surface in the area to be measured
Surface grids data；
According to the location information of the location information of the CCD camera and the plane mirror, calculated using mirror-reflection principle
Obtain the location information of the CCD camera picture in the plane mirror；
By the location information of the optical fiber head, the location information of the CCD camera, the CCD camera picture location information, described
Single width laser image, the single width fluorescent image and the body surface mesh data are input in the three-dimensional reconstruction software, meter
Calculation obtains the three-dimensional fluorescence image.
6. fluorescent scattering optical imaging method as claimed in claim 5, which is characterized in that further include: before the CCD camera
Fluorescent optical filter is set and filters out the fluorescence that the fluorescent material issues；CCD camera acquires recombination laser above the objective table
Image, comprising:
The CCD camera, which directly acquires, is emitted by the laser fiber head and is passed through the laser of the determinand, generates first
Laser image, while acquiring across the determinand and the laser that is reflected through the plane mirror, second laser image is generated,
Recombination laser image described in the first laser image and second laser image construction.
7. fluorescent scattering optical imaging method as claimed in claim 5, which is characterized in that further include: before the CCD camera
The laser that Excitation Filter with High filters out the laser fiber head outgoing is set；CCD camera acquires compound above the objective table
Fluorescent image, comprising:
The CCD camera directly acquires the fluorescence issued by the fluorescent material in the area to be measured, generates the first fluorescent image, together
When acquire fluorescence that fluorescent material in the area to be measured issues and the reflection through the plane mirror, generate the second fluorescence
Image, first fluorescent image and the second fluorescent image constitute the composite fluorescence image.
8. fluorescent scattering optical imaging method as claimed in claim 5, which is characterized in that believe the position of the optical fiber head
Breath, the location information of the CCD camera, the location information of the CCD camera picture, the single width laser image, the single width are glimmering
Light image and the body surface mesh data are input in the three-dimensional reconstruction software, and the three-dimensional fluorescence image is calculated,
Include:
The laser image and fluorescent image scaling are matched to the actual size in the area to be measured；
The location information of the fluorescent image, the optical fiber head after the laser image, scaling after scaling matching is matched,
The location information of the CCD camera, the location information of the CCD camera picture and the body surface mesh data are input to described three
It ties up in reconstruction software, the three-dimensional fluorescence image is calculated.
9. fluorescent scattering optical imaging method as claimed in claim 5, which is characterized in that the patch on one side of the plane mirror
Together on the objective table；
The recombination laser image and composite fluorescence image are cut into multiple single width laser images and multiple single width fluorescence respectively
Image, comprising:
Intersection along plane where plane where the plane mirror and the objective table is by the recombination laser image cutting-out
At multiple single width laser images；
Intersection along plane where plane where the plane mirror and the objective table is by the composite fluorescence image cutting-out
At multiple single width fluorescent images.
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| CN101810465A (en) * | 2009-02-19 | 2010-08-25 | 广州中科恺盛医疗科技有限公司 | Three-dimensional imaging data acquisition device |
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