CN106404736A - Special detection method and equipment for estradiol in duck meat - Google Patents
Special detection method and equipment for estradiol in duck meat Download PDFInfo
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- CN106404736A CN106404736A CN201611078775.6A CN201611078775A CN106404736A CN 106404736 A CN106404736 A CN 106404736A CN 201611078775 A CN201611078775 A CN 201611078775A CN 106404736 A CN106404736 A CN 106404736A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention discloses a special detection method and equipment for estradiol in duck meat. The special detection method comprises the following steps: step 1, sampling a duck meat sample: washing fresh duck meat with clean water, and cutting two pieces of samples with the size of 5cm*5cm along a meridian and collateral direction of the duck meat through a stainless steel cutter; taking one piece for carrying out experiment detection and taking the other piece for later use; putting the two pieces of duck meat samples into a food-grade stirrer respectively, and mashing the two pieces of duck meat samples at the speed of 10 circles per second; adding a small amount of clean water in a stirring process. According to the special detection method, after the duck meat is mashed, the duck meat is at a supported liquid state and is mixed with a nano-gold solution; an aptamer covering nano-gold is specifically combined with the estradiol, so that free nano-gold is released; the free nano-gold is gathered; rhodamine is added and has no quenching effect on gathered nano-gold fluorescence light; the nano-gold has a very strong fluorescence light quenching effect on the nano-gold at a free state; the detection method has the advantages of simplicity in operation, short detection time and relatively low cost.
Description
Technical field
The present invention relates to technical field of food detection, the dedicated test method of estradiol and its set in specially a kind of duck
Standby.
Background technology
Estradiol is a kind of natural estrogen, and is widely present in natural environment, and it can enter from external environment condition
Entering human body, thus upsetting human endocrine systemic-function, causing human body in systems such as nervous system, immune system and reproductive developments
On pathology, women absorbs excessive estradiol and is easily caused and develops in advance, and the male sex absorbs excessive estradiol and easily occurs the
Two sex character, some animals or poultry, such as family duck, illegal raiser is a duck of accelerating the ripening in advance, shortens the cultivation phase, is entered using estradiol
Row accelerates the ripening, therefore develop a kind of simple, efficiently, the detection method that is sensitively directed to estradiol is significant.
The detection method of traditional estradiol has high performance liquid chromatography, gas chromatography-mass spectrography, Chemiluminescence immunoassay
Etc., although these methods can reach higher test limit, it is all based on the instrument of complex and expensive in laboratory, and
High cost, time-consuming it is difficult to meet the needs of live real-time and precise detection, and the present invention provides one kind to utilize cation surface activating
The method that nm of gold colorimetric detection estradiol is assembled in agent.
Content of the invention
（One）The technical problem solving
For the deficiencies in the prior art, the invention provides the dedicated test method of estradiol and its equipment in a kind of duck, solution
Existing estradiol detection method of having determined and the problem that equipment cost is high and time-consuming.
（Two）Technical scheme
For achieving the above object, the present invention provides following technical scheme：A kind of dedicated test method of estradiol in duck, including
Following steps：
First, take duck sample：Meat for fresh duck clear water is cleaned, extracts two pieces with stainless steel knife along duck channels and collaterals direction
5*5 centimetre of sample, one piece is used for experiment detection, and one piece, for standby, two pieces of duck samples is individually placed to food-grade stirring
Respectively two pieces of duck samples are blended with the speed of per second 10 circles in machine, and add a small amount of clear water during stirring, prevent
Only duck is pasted onto inside mixer, after duck blends, is taken out two pieces of duck samples respectively with clean spoon, is placed on
In clean glassware, it is temporarily placed in refrigeration 5-8 minute in refrigerator or refrigerator-freezer, temperature is set to 5-8 degree Celsius；
2nd, control sample is set：Take a clean glassware, and with marker, A is labeled as on glassware, to glass
Add the pure water of 100-120 milliliter in vessel, then two parts of duck samples being obtained in step one are taken from refrigerator or refrigerator-freezer
Go out portion, another is wrapped on duck sample using preservative film, continue refrigeration, get up as redundant detection sample deposit, take
The duck sample going out is held with clean glassware, and is labeled as B on glassware with marker, adds in B vessel
The pure water of 20-30 milliliter, with clean stirring spoon, the duck blending sample is stirred so as to fully be melted with pure water
Close；
3rd, start to test；S1, add the adaptation liquid solution of equivalent respectively in A, B vessel, and mixed with stirring spoon respectively
With incubation 5 minutes, obtain mixed liquor A 1, B1；
S2, add two sweet fat reagent of the quality such as appropriate respectively in A1 and B1 respectively, and mixed with stirring spoon respectively,
Standing 1 minute, more respectively in A1, B1 add etc. quality methyl propane sulfonic acid reagent, and respectively carried out with stirring spoon mix and
Incubation 5 minutes, obtains mixed liquor A 2, B2；
The nano-Au solution of the quality such as S3, the 30-40 milliliter adding respectively in A2, B2, and mixed with stirring spoon respectively,
Obtain mixed liquor A 3, B3；
S4, the sodium chloride adding the quality such as 15-20 milliliter in A3, B3 respectively and potassium chloride mixed liquor, and respectively with stirring spoon
Mixed, obtained mixed liquor A 4 and B4；
S5, take the quartzy fluorescence cuvette two that internal diameter is that 60*60 height is 60 millimeters, and be respectively labeled as with marker
A5 and B5, cleans and allows it naturally to dry, after drying, A4 and B4 mixed liquor is drained in A5 and B5 with mozzle respectively, and
Two quartzy fluorescence cuvette of A5 and B5 are placed in sepectrophotofluorometer, switch on power, into sepectrophotofluorometer
A5 and B5 finally respectively add 50-60 milliliter etc. quality rhodamine liquor, open sepectrophotofluorometer be scanned survey
Determine signal.
Preferably, the condition of scanning of described sepectrophotofluorometer is：Optical slits is excited to be 9 nanometers, transmitting optical slits is 2
Nanometer, a length of 480 nanometers of excitation light wave, it is scanned with this understanding, obtains its fluorescence spectrum signal, the fluorescence of A5 and B5 is strong
Degree signal is respectively C1 and C2, calculates the difference that enhanced fluorescence intensity level is C2 and C1.
Preferably, the preparation method of described nano-Au solution is as follows：In 100 milliliters of the mass percent concentrations boiling it is
In 0.015% chlorauric acid solution, 4 milliliters of mass percent concentrations of addition are 1% citric acid three sodium solution, stir 30 minutes,
Continue stirring mixture 15 minutes after removing heater, finally, make solution reach 25 degrees Celsius and using 0.15 micron
Ultrafiltration membrance filter, is then stored at standby in the black glass bottle that constant temperature is 4 degrees Celsius, thus nano-Au solution is obtained.
Preferably, the concentration of described sodium chloride is 0.14mol/L, and the concentration of rhodamine is 8umol/L, and methyl propane sulfonic acid tries
The concentration of agent is 20umol/L, and methyl propane sulfonic acid reagent is the effect as buffer solution, can also be replaced using ethyl propane sulfonic acid
Methyl propane sulfonic acid reagent.
Preferably, in described duck, the preparation sample detection system involved by estradiol detection method is：Take 10 milliliters of B5
Testing sample, is added in centrifuge tube, is placed in centrifuge tube after being incubated 25 minutes under 25 degrees celsius after fully mixing again,
Add the sodium chloride solution that 30 milliliters of concentration are 0.14mol/L in mixed liquor and rhodamine that 25 milliliters of concentration is 8umol/L is molten
Liquid, mixes, then according to the calculation that C2 subtracts C1 obtains enhanced fluorescence intensity level.
Preferably, in described duck estradiol special inspecting equipment, including body, in body, be installed with slide bar,
On described slide bar, slip cap is connected to slide, and the bottom of described slide is fixedly connected with laser scanner, is provided with described body
Monitor station, the right side at described monitor station top offers and is fixedly connected with beam in groove and groove, the top of described beam
Portion is provided with two vessel, and is separated by dividing plate between two vessel, and the top of described body inwall is installed with shading
Plate, the both sides of described body are all installed with vent window, and the bottom of described body is installed with base.
（Three）Beneficial effect
The invention provides in a kind of duck estradiol dedicated test method.Possesses following beneficial effect：
（1）, the present invention is by blending rear support liquid state by duck and mixing with nano-Au solution so that estradiol aptamers bag
It is rolled in the surface of nm of gold, when there is estradiol in duck, the aptamers being wrapped in nm of gold can be with estradiol specificity
In conjunction with, thus discharging free nm of gold, after adding sodium chloride and Klorvess Liquid, free nm of gold produces assembles,
Luo Jindan is added not have quenching effect to the nm of gold fluorescence assembled, when not adding rhodamine in detectable substance, at nm of gold
In free state, and nm of gold has very strong fluorescence quenching for the nm of gold of free state, and test is right by arranging
The mode of ratio, plays very intuitively contrast effect, has and clearly distinguishes in duck the whether effect containing estradiol, and
By fluorescence to specific strength, also can determine whether the concentration level of contained estradiol in duck, and this detection method has operation
Simply, detect and take short, lower-cost advantage.
Brief description
Fig. 1 is present configuration schematic diagram.
In figure：1 body, 2 slide bars, 3 slides, 4 laser scanners, 5 monitor stations, 6 beams, 7 vessel, 8 dividing plates, 9 shadings
Plate, 10 vent windows, 11 bases.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of not making creative work
Embodiment, broadly falls into the scope of protection of the invention.
As shown in figure 1, the present invention provides a kind of technical scheme：A kind of dedicated test method of estradiol in duck, including
Following steps：
First, take duck sample：Meat for fresh duck clear water is cleaned, extracts two pieces with stainless steel knife along duck channels and collaterals direction
5*5 centimetre of sample, one piece is used for experiment detection, and one piece, for standby, two pieces of duck samples is individually placed to food-grade stirring
Respectively two pieces of duck samples are blended with the speed of per second 10 circles in machine, and add a small amount of clear water during stirring, prevent
Only duck is pasted onto inside mixer, after duck blends, is taken out two pieces of duck samples respectively with clean spoon, is placed on
In clean glassware, it is temporarily placed in refrigeration 5-8 minute in refrigerator or refrigerator-freezer, temperature is set to 5-8 degree Celsius；
2nd, control sample is set：Take a clean glassware, and with marker, A is labeled as on glassware, to glass
Add the pure water of 100-120 milliliter in vessel, then two parts of duck samples being obtained in step one are taken from refrigerator or refrigerator-freezer
Go out portion, another is wrapped on duck sample using preservative film, continue refrigeration, get up as redundant detection sample deposit, take
The duck sample going out is held with clean glassware, and is labeled as B on glassware with marker, adds in B vessel
The pure water of 20-30 milliliter, with clean stirring spoon, the duck blending sample is stirred so as to fully be melted with pure water
Close；
3rd, start to test；S1, add the adaptation liquid solution of equivalent respectively in A, B vessel, and mixed with stirring spoon respectively
With incubation 5 minutes, obtain mixed liquor A 1, B1；
S2, add two sweet fat reagent of the quality such as appropriate respectively in A1 and B1 respectively, and mixed with stirring spoon respectively,
Standing 1 minute, more respectively in A1, B1 add etc. quality methyl propane sulfonic acid reagent, and respectively carried out with stirring spoon mix and
Incubation 5 minutes, obtains mixed liquor A 2, B2；
The nano-Au solution of the quality such as S3, the 30-40 milliliter adding respectively in A2, B2, the preparation method of nano-Au solution is such as
Under：It is in 0.015% chlorauric acid solution in 100 milliliters of the mass percent concentrations boiling, add 4 milliliters of mass percents
Concentration is 1% citric acid three sodium solution, stirs 30 minutes, continues stirring mixture 15 minutes after removing heater, finally,
Make solution reach 25 degrees Celsius and the ultrafiltration membrance filter using 0.15 micron, be then stored at the black that constant temperature is 4 degrees Celsius
Standby in vial, thus nano-Au solution is obtained, and mixed with stirring spoon respectively, obtained mixed liquor A 3, B3；
S4, the sodium chloride adding the quality such as 15-20 milliliter in A3, B3 respectively and potassium chloride mixed liquor, the concentration of sodium chloride is
0.14mol/L, the concentration of rhodamine is 8umol/L, and the concentration of methyl propane sulfonic acid reagent is 20umol/L, methyl propane sulfonic acid reagent
It is the effect as buffer solution, methyl propane sulfonic acid reagent can also be replaced using ethyl propane sulfonic acid, and be carried out with stirring spoon respectively
Mix, obtain mixed liquor A 4 and B4；
S5, take the quartzy fluorescence cuvette two that internal diameter is that 60*60 height is 60 millimeters, and be respectively labeled as with marker
A5 and B5, cleans and allows it naturally to dry, after drying, A4 and B4 mixed liquor is drained in A5 and B5 with mozzle respectively, and
Two quartzy fluorescence cuvette of A5 and B5 are placed in sepectrophotofluorometer, switch on power, into sepectrophotofluorometer
A5 and B5 finally add respectively 50-60 milliliter etc. quality rhodamine liquor, involved by estradiol detection method in duck
Preparing sample detection system is：Take 10 milliliters of B5 testing samples, be added in centrifuge tube, again centrifuge tube is placed in after fully mixing
After incubation 25 minutes under 25 degrees celsius, add in mixed liquor the sodium chloride solution that 30 milliliters of concentration are 0.14mol/L and
25 milliliters of concentration are the rhodamine liquor of 8umol/L, mix, then according to C2 subtracts the calculation of C1, and to obtain enhanced fluorescence strong
Angle value, opens sepectrophotofluorometer and is scanned measuring signal, the condition of scanning of sepectrophotofluorometer is：Excite optical slits
For 9 nanometers, transmitting optical slits is 2 nanometers, and a length of 480 nanometers of excitation light wave is scanned with this understanding, obtains its fluorescence light
Spectrum signal, the fluorescence intensity signals of A5 and B5 are respectively C1 and C2, calculate the difference that enhanced fluorescence intensity level is C2 and C1, duck
The special inspecting equipment of estradiol in meat, including body 1, is installed with slide bar 2, on slide bar 2, slip cap is connected to cunning in body 1
Seat 3, slide bar 2 can freely slide on slide 3, and the bottom of slide 3 is fixedly connected with laser scanner 4, is provided with body 1
Monitor station 5, the right side at monitor station 5 top offers and is fixedly connected with beam 6 in groove and groove, and beam 6 plays damping
Effect, the top of beam 6 is provided with two vessel 7, is loaded with duck sample and blank respectively in two vessel 7
Separated by dividing plate 8 between sample, and two vessel 7, the top of body 1 inwall is installed with shadow shield 9, arranges shadow shield 9
Play and strengthen the effect that fluorescent brightness shows, the both sides of body 1 are all installed with vent window 10, the fixing peace in bottom of body 1
Equipped with base 11.
During use, the present invention is by blending rear support liquid state by duck and mixing with nano-Au solution so that estradiol
Aptamers are wrapped in the surface of nm of gold, and when there is estradiol in duck, the aptamers being wrapped in nm of gold can be with female two
Alcohol specifically binds, thus discharging free nm of gold, after adding sodium chloride and Klorvess Liquid, free nm of gold is produced
Raw gathering, adds Luo Jindan not have quenching effect to the nm of gold fluorescence assembled, when not adding rhodamine in detectable substance, receives
Meter Jin is in free state, and nm of gold has very strong fluorescence quenching for the nm of gold of free state, and test is passed through
The mode of setting comparative example, plays very intuitively contrast effect, has and clearly distinguishes in duck the whether effect containing estradiol
Really, and by fluorescence to specific strength, also can determine whether the concentration level of contained estradiol in duck, and this detection method tool
There is simple to operate, time-consuming short, the lower-cost advantage of detection.
To sum up can obtain, the special inspecting equipment of estradiol in this duck, by arranging slide bar 2, slide 3, laser scanner
4th, dividing plate 8 and shadow shield 9, solves the problems, such as existing estradiol detection method and equipment cost is high and time-consuming.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation are made a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " inclusion ", "comprising" or its any other variant are intended to
Comprising of nonexcludability, wants so that including a series of process of key elements, method, article or equipment and not only including those
Element, but also include other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element that limited by sentence "including a ..." it is not excluded that
Also there is other identical element including in the process of described key element, method, article or equipment.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understand and can carry out multiple changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (6)
1. in a kind of duck the dedicated test method of estradiol it is characterised in that comprising the following steps：
Take duck sample：Meat for fresh duck clear water is cleaned, extracts two pieces of 5*5 with stainless steel knife along duck channels and collaterals direction
Centimetre sample, one piece be used for experiment detection, one piece be used for standby, two pieces of duck samples are individually placed in food-grade mixer
Respectively two pieces of duck samples are blended with the speed of per second 10 circles, and add a small amount of clear water during stirring, prevent duck
Meat is pasted onto inside mixer, after duck blends, is taken out two pieces of duck samples respectively with clean spoon, is placed on clean
Glassware in, be temporarily placed in refrigerator or refrigerator-freezer refrigeration 5-8 minute, temperature is set to 5-8 degree Celsius；
Setting control sample：Take a clean glassware, and with marker, A is labeled as on glassware, to vierics
Add the pure water of 100-120 milliliter in ware, then two parts of duck samples being obtained in step one are taken out from refrigerator or refrigerator-freezer
Portion, another is wrapped on duck sample using preservative film, continues refrigeration, gets up as redundant detection sample deposit, takes out
Duck sample held with clean glassware, and with marker, B is labeled as on glassware, adds 20- in B vessel
30 milliliters of pure water, with clean stirring spoon, the duck blending sample is stirred so as to fully be merged with pure water；
Start to test；S1, respectively in A, B vessel add equivalent adaptation liquid solution, and respectively carried out with stirring spoon mix and
Incubation 5 minutes, obtains mixed liquor A 1, B1；
S2, add two sweet fat reagent of the quality such as appropriate respectively in A1 and B1 respectively, and mixed with stirring spoon respectively,
Standing 1 minute, more respectively in A1, B1 add etc. quality methyl propane sulfonic acid reagent, and respectively carried out with stirring spoon mix and
Incubation 5 minutes, obtains mixed liquor A 2, B2；
The nano-Au solution of the quality such as S3, the 30-40 milliliter adding respectively in A2, B2, and mixed with stirring spoon respectively,
Obtain mixed liquor A 3, B3；
S4, the sodium chloride adding the quality such as 15-20 milliliter in A3, B3 respectively and potassium chloride mixed liquor, and respectively with stirring spoon
Mixed, obtained mixed liquor A 4 and B4；
S5, take the quartzy fluorescence cuvette two that internal diameter is that 60*60 height is 60 millimeters, and be respectively labeled as with marker
A5 and B5, cleans and allows it naturally to dry, after drying, A4 and B4 mixed liquor is drained in A5 and B5 with mozzle respectively, and
Two quartzy fluorescence cuvette of A5 and B5 are placed in sepectrophotofluorometer, switch on power, into sepectrophotofluorometer
A5 and B5 finally respectively add 50-60 milliliter etc. quality rhodamine liquor, open sepectrophotofluorometer be scanned survey
Determine signal.
2. in a kind of duck according to claim 1 the dedicated test method of estradiol it is characterised in that described fluorescence divides
The photometric condition of scanning of light is：Optical slits is excited to be 9 nanometers, transmitting optical slits is 2 nanometers, a length of 480 nanometers of excitation light wave,
It is scanned with this understanding, obtains its fluorescence spectrum signal, the fluorescence intensity signals of A5 and B5 are respectively C1 and C2, calculate and increase
Strong fluorescence intensity level is the difference of C2 and C1.
3. in a kind of duck according to claim 1 the dedicated test method of estradiol it is characterised in that described nm of gold
The preparation method of solution is as follows：It is in 0.015% chlorauric acid solution in 100 milliliters of the mass percent concentrations boiling, add
4 milliliters of mass percent concentrations are 1% citric acid three sodium solution, stir 30 minutes, continue stirring mixing after removing heater
Thing 15 minutes, finally, makes solution reach 25 degrees Celsius and the ultrafiltration membrance filter using 0.15 micron, being then stored at constant temperature is
Standby in 4 degrees Celsius of black glass bottle, thus nano-Au solution is obtained.
4. in a kind of duck according to claim 1 the dedicated test method of estradiol it is characterised in that described sodium chloride
Concentration be 0.14mol/L, the concentration of rhodamine is 8umol/L, and the concentration of methyl propane sulfonic acid reagent is 20umol/L, methyl-prop
Sulfonic acid reagent is the effect as buffer solution, can also replace methyl propane sulfonic acid reagent using ethyl propane sulfonic acid.
5. in a kind of duck according to claim 1 and 2 the dedicated test method of estradiol it is characterised in that described duck
In meat, the preparation sample detection system involved by estradiol detection method is：Take 10 milliliters of B5 testing samples, be added to centrifuge tube
In, again centrifuge tube is placed in after fully mixing after being incubated 25 minutes under 25 degrees celsius, in mixed liquor, 30 milliliters of addition is dense
Spending the sodium chloride solution for 0.14mol/L and 25 milliliters of concentration is the rhodamine liquor of 8umol/L, mixes, then according to C2 subtracts
The calculation of C1 obtains enhanced fluorescence intensity level.
6. in a kind of duck according to claim 1 estradiol dedicated test method, its special equipment includes body
（1）It is characterised in that body（1）Inside it is installed with slide bar（2）, described slide bar（2）Upper slip cap is connected to slide（3）, described
Slide（3）Bottom be fixedly connected with laser scanner（4）, described body（1）Inside it is provided with monitor station（5）, described monitor station
（5）The right side at top offers in groove and groove and is fixedly connected with beam（6）, described beam（6）Top be provided with
Two vessel（7）, and two vessel（7）Between pass through dividing plate（8）Separate, described body（1）The top of inwall is installed with
Shadow shield（9）, described body（1）Both sides be all installed with vent window（10）, described body（1）Bottom be installed with
Base（11）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078775.6A CN106404736A (en) | 2016-11-30 | 2016-11-30 | Special detection method and equipment for estradiol in duck meat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078775.6A CN106404736A (en) | 2016-11-30 | 2016-11-30 | Special detection method and equipment for estradiol in duck meat |
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| Publication Number | Publication Date |
|---|---|
| CN106404736A true CN106404736A (en) | 2017-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611078775.6A Pending CN106404736A (en) | 2016-11-30 | 2016-11-30 | Special detection method and equipment for estradiol in duck meat |
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| CN104597258A (en) * | 2015-01-09 | 2015-05-06 | 上海交通大学 | Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer |
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| CN105021580A (en) * | 2015-06-16 | 2015-11-04 | 上海应用技术学院 | 17beta-estradiol detection method |
| CN105044321A (en) * | 2015-06-23 | 2015-11-11 | 上海交通大学 | 17 beta-estradiol colorimetric detecting method based on nanogold collected by surfactant |
| CN105424606A (en) * | 2015-12-28 | 2016-03-23 | 江南大学 | Multifunctional opto-acoustic, fluorescence microscopic and fluorescence spectrum imaging analytical device and method |
| CN105873501A (en) * | 2016-03-24 | 2016-08-17 | 中国科学院深圳先进技术研究院 | A fluorescent scattering optical imaging system and method |
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2016
- 2016-11-30 CN CN201611078775.6A patent/CN106404736A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597258A (en) * | 2015-01-09 | 2015-05-06 | 上海交通大学 | Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer |
| CN104677869A (en) * | 2015-02-05 | 2015-06-03 | 上海应用技术学院 | Method for detecting 17bata-estradiol by using resonance scattering spectrometry based on functional nucleic acids |
| CN105021580A (en) * | 2015-06-16 | 2015-11-04 | 上海应用技术学院 | 17beta-estradiol detection method |
| CN105044321A (en) * | 2015-06-23 | 2015-11-11 | 上海交通大学 | 17 beta-estradiol colorimetric detecting method based on nanogold collected by surfactant |
| CN105424606A (en) * | 2015-12-28 | 2016-03-23 | 江南大学 | Multifunctional opto-acoustic, fluorescence microscopic and fluorescence spectrum imaging analytical device and method |
| CN105873501A (en) * | 2016-03-24 | 2016-08-17 | 中国科学院深圳先进技术研究院 | A fluorescent scattering optical imaging system and method |
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