CN101805321A - Method for extracting dihydromyricetin from vine tea - Google Patents
Method for extracting dihydromyricetin from vine tea Download PDFInfo
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- CN101805321A CN101805321A CN 201010149014 CN201010149014A CN101805321A CN 101805321 A CN101805321 A CN 101805321A CN 201010149014 CN201010149014 CN 201010149014 CN 201010149014 A CN201010149014 A CN 201010149014A CN 101805321 A CN101805321 A CN 101805321A
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Abstract
The invention discloses a method for extracting dihydromyricetin from vine tea, including the following steps: (1) vine tea dry leaves are weighed, subject to microwave radiation treatment by a microwave oven and then smashed; (2) the particles are added into a microwave reverse flow extractor, water is added, microwave extraction and filtering are carried out, thus obtaining filtration A and residue A; (3) the residue A is processed according to the method in the step (2), thus obtaining filtration B and residue B; (4) residue A and residue B are merged and added into the microwave reverse flow extractor, ethanol is added, microwave extraction is carried out for 30-60min and then filtering is carried out, thus obtaining filtration C; (5) the filtrations A, B and C are merged, and then concentration, crystallization and drying are carried out. The invention has simple technology and convenient operation, extraction ratio of dihydromyricetin can reach 47.3%, purity can reach 85%, the required organic solvent is less, operating safety is good, no high temperature furnace is required for heating, energy consumption is reduced, and production cost is saved.
Description
Technical field
The present invention relates to extraction of effective components in a kind of vine tea, be specifically related to a kind of method of from vine tea, extracting dibydro myricetrin.
Background technology
Vine tea is a kind of very ancient Chinese herbal medicine resource, class tea plant resources and medicine food dual purpose plant resource.Its former plant is the Ampelopsis grossedentata of Vitaceae Ampelopsis, is commonly called as sweet tree tea, sweet tea rattan, field mother-in-law's tea (Guangxi), rattan mother-in-law tea (Lianzhou City, Guangdong), wintersweet tea, parmelia saxatilis tea (Guangdong Germany and Britain), the mould tea of Mao Yan (Zhangjiajie, Hunan), white tea (Guangdong), white tea cake, leprosy dysentery tea, manna tea etc.It is cool in nature, and tool is clearing heat and detoxicating.Diuresis.Anti-inflammatory is dispelled rheumatism, and effects such as the broken knot of the stasis of blood of loosing can be cured diseases such as lung carbuncle, intestines, scrofula disease, rheumatism, carbuncle disease swell and ache, fall dozen, scald, chronic nephritis, hepatitis, difficulty and pain in micturition, and can control diseases such as gastropyretic vomiting, flu, swelling and pain in the throat.In the last few years, Chinese scholar had been done than systematic research work to vine tea.And obtained certain achievement in research, and especially in separation, the evaluation of its composition, the research aspect of extraction process and pharmacological function.But development and use and clinical application research to vine tea still rest on general lower level.
In vine tea, crude protein content 13.94%, water-soluble protein content 0.55%, amino acid total content 2_3%. contains 17 seed amino acids. wherein comprise 8 seed amino acids and the special propylhomoserins such as ^y one aminobutyric acid, methionine(Met) of needed by human, total percent of ash 6.0%, total polyphenols 18.5%, inorganic nutritive element such as Fe, Cu, Zn, Mg, Mn, Se, Na, F and worker's content is than green tea height commonly used; The water extraction content height (nearly 50%) of its spring, summer children's young stem and leaf.Wherein water-soluble sugar content is about 10%; Aminoacids content is about 5%: polyphenolic compound content is about 20%; Flavonoid content is about 1.2%, and wherein dihydromyricetin content is about 30%.
Dibydro myricetrin is a kind of wild bejuco of Vitaceae Ampelopsis, and wherein main active ingredient is a flavonoid compound, and this type of material has multiple peculiar effects such as removing free radical, anti-oxidant, antithrombotic, antitumor, anti-inflammatory; And dibydro myricetrin is comparatively special a kind of flavonoid compound, except that the general characteristic with flavonoid compound, also has the effects such as sickness rate of removing alcoholism, prevention alcoholic liver, fatty liver, the deterioration of inhibition liver cell, reducing liver cancer.Be liver protecting, the non-defective unit of Dealcoholic sobering-up.
Summary of the invention
The object of the present invention is to provide a kind of easy and simple to handle, extract yield is high extracts the method for dibydro myricetrin from vine tea.
The method of extracting dibydro myricetrin from vine tea of the present invention comprises the steps:
(1) take by weighing the vine tea cured leaf, carry out microwave radiation processing with microwave oven after, be crushed to 80-100 purpose particle;
(2) above-mentioned particle is joined in the microwave reflux extracting device, add entry again, microwave extraction 30-90 minute after-filtration gets filtrate A, filter residue A;
(3) filter residue A is handled with the method for step (2), get liquor B, filter residue B;
(4) merge filter residue A and filter residue B, join in the microwave reflux extracting device, add ethanol again, microwave extraction 30-60 minute after-filtration gets liquor C;
(5) merging filtrate A, liquor B and liquor C concentrate crystallization, drying.
Wherein, the mass ratio of the water that is added in vine tea cured leaf described in the step (1) and the step (2) is preferably 1: 5-15; The content of dibydro myricetrin in raw material has very big-difference with the different of raw material sources, quality and period of storage, thus the mass ratio of the water that is added in vine tea cured leaf described in the step of choosing (1) and the step (2) be 1: 10 for best;
Step (3) can repeat 2-3 time, but multiplicity is too much, then also can extract the impurity of more amount with the proposition two of required effective constituent, so carry 2 times for good with water;
The ethanol massfraction is preferably 70% described in the step (4);
Ethanol quality described in the step (4) be filter residue A and filter residue B total mass 2-5 doubly;
The method of from vine tea, extracting dibydro myricetrin of the present invention, employed microwave power is 800-1500w, adopts the microwave can the penetration heating, and extraction time is saved greatly, efficient height, output are big; Micro-wave energy has the extractability of overlength, and same raw material needs twice or thrice with the method for routine could defecation, can a defecation under microwave field, simplify technical process greatly; Microwave extraction does not have thermal inertia, is easy to control.
The method of from vine tea, extracting dibydro myricetrin of the present invention, technology is simple, easy to operate, the extraction yield of dibydro myricetrin can reach 47.3%, and purity reaches 85%, and it is few to extract required organic solvent usage quantity in this method, good operation safety, and do not need the High Temperature Furnaces Heating Apparatus heating, reduced energy consumption, saved production cost.
Embodiment
Following examples are to the further specifying of flesh and blood of the present invention, and are not limitation of the present invention.
Embodiment 1
(1) takes by weighing vine tea cured leaf 10kg, after carrying out microwave radiation processing under the microwave power 900w, be crushed to 80-100 purpose particle with microwave oven;
(2) above-mentioned particle is joined in the WNT-B type microwave reflux extracting device, add 100kg water again, extract 60 minutes after-filtration down, get filtrate A, filter residue A in microwave power 1000w;
(3) filter residue A is joined in the WNT-B type microwave reflux extracting device again, add 100kg water, extract 60 minutes after-filtration down, get liquor B, filter residue B in microwave power 1000w;
(4) merge filter residue A and filter residue B 5.3kg altogether, join in the microwave reflux extracting device, add the ethanol of 15kg massfraction 70% again, microwave power 1000w extracts 50 minutes after-filtration down, gets liquor C;
(5) merging filtrate A, liquor B and liquor C concentrate, crystallization, and drying obtains pure dibydro myricetrin, extraction yield 43.6%, purity 83.6%.
Embodiment 2
(1) takes by weighing vine tea cured leaf 10kg, after carrying out microwave radiation processing under the microwave power 800w, be crushed to 80-100 purpose particle with microwave oven;
(2) above-mentioned particle is joined in the WNT-B type microwave reflux extracting device, add 60kg water again, extract 30 minutes after-filtration down, get filtrate A, filter residue A in microwave power 1500w;
(3) filter residue A is joined in the WNT-B type microwave reflux extracting device again, add 60kg water, extract 30 minutes after-filtration down, get liquor B, filter residue B in microwave power 1500w;
(4) merge filter residue A and filter residue B 5.1kg altogether, join in the microwave reflux extracting device, add the ethanol of 12kg massfraction 70% again, microwave power 1500w extracts 30 minutes after-filtration down, gets liquor C;
(5) merging filtrate A, liquor B and liquor C concentrate, crystallization, and drying obtains pure dibydro myricetrin, extraction yield 47.3%, purity 85.0%.
Embodiment 3
(1) takes by weighing vine tea cured leaf 10kg, after carrying out microwave radiation processing under the microwave power 800w, be crushed to 80-100 purpose particle with microwave oven;
(2) above-mentioned particle is joined in the WNT-B type microwave reflux extracting device, add 120kg water again, extract 90 minutes after-filtration down, get filtrate A, filter residue A in microwave power 1200w;
(3) filter residue A is joined in the WNT-B type microwave reflux extracting device again, add 120kg water, extract 90 minutes after-filtration down, get liquor B, filter residue B in microwave power 1200w;
(4) merge filter residue A and filter residue B 5.5kg altogether, join in the microwave reflux extracting device, add the ethanol of 18kg massfraction 50% again, microwave power 1200w extracts 30 minutes after-filtration down, gets liquor C;
(5) merging filtrate A, liquor B and liquor C concentrate, crystallization, and drying obtains pure dibydro myricetrin, extraction yield 45.7%, purity 81.3%.
Claims (5)
1. method of extracting dibydro myricetrin from vine tea is characterized in that adopting following step:
(1) take by weighing the vine tea cured leaf, carry out microwave radiation processing with microwave oven after, be crushed to 80-100 purpose particle;
(2) above-mentioned particle is joined in the microwave reflux extracting device, add entry again, microwave extraction 30-90 minute after-filtration gets filtrate A, filter residue A;
(3) filter residue A is handled with the method for step (2), get liquor B, filter residue B;
(4) merge filter residue A and filter residue B, join in the microwave reflux extracting device, add ethanol again, microwave extraction 30-60 minute after-filtration gets liquor C;
(5) merging filtrate A, liquor B and liquor C concentrate crystallization, drying.
2. the method for extracting dibydro myricetrin from vine tea according to claim 1 is characterized in that the mass ratio of the water that added in vine tea cured leaf described in the step (1) and the step (2) is 1: 5-15.
3. the method for extracting dibydro myricetrin from vine tea according to claim 1 is characterized in that the ethanol massfraction is 70% described in the step (4).
4. the method for from vine tea, extracting dibydro myricetrin according to claim 3, it is characterized in that ethanol quality described in the step (4) be filter residue A and filter residue B total mass 2-5 doubly.
5. according to each described method of from vine tea, extracting dibydro myricetrin among the claim 1-4, it is characterized in that described microwave power is 800-1500w.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010101490141A CN101805321B (en) | 2010-04-16 | 2010-04-16 | Method for extracting dihydromyricetin from vine tea |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101490141A CN101805321B (en) | 2010-04-16 | 2010-04-16 | Method for extracting dihydromyricetin from vine tea |
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| CN101805321A true CN101805321A (en) | 2010-08-18 |
| CN101805321B CN101805321B (en) | 2012-08-22 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106931A (en) * | 2010-12-30 | 2011-06-29 | 张家界茅岩莓有限公司 | Method for producing diverse extracts of berry tea |
| CN102584766A (en) * | 2011-12-31 | 2012-07-18 | 郁建平 | Method for simultaneously separating dihydromyricetin and myricetin from ampelopsis plant |
| CN102775455A (en) * | 2011-05-13 | 2012-11-14 | 贵州省生物研究所 | Dihydromyricetin medicine treating acute and chronic bronchitis and its preparation method |
| CN102796069A (en) * | 2012-07-04 | 2012-11-28 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN104876903A (en) * | 2014-02-27 | 2015-09-02 | 天津药物研究院有限公司 | Crystallization form of dihydromyricetin, preparation method thereof and pharmaceutical composition containing the same |
| CN105010634A (en) * | 2015-07-06 | 2015-11-04 | 河南科技大学 | Moyeam tea cream and preparation method thereof |
| CN109053666A (en) * | 2018-09-05 | 2018-12-21 | 湖南中茂生物科技有限公司 | A kind of extracting method of dihydromyricetin |
| CN109349391A (en) * | 2018-12-20 | 2019-02-19 | 株洲千金药业股份有限公司 | A kind of preparation method and application of the long-lived vine tea of activity |
| CN113480508A (en) * | 2021-08-25 | 2021-10-08 | 武汉食味深山农业科技有限公司 | Extraction process of dihydromyricetin in vine tea and preparation process of vine tea functional beverage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712402A (en) * | 2004-06-25 | 2005-12-28 | 广东省农业科学院蚕业与农产品加工研究所 | Extraction of dihydromyricetin from microwave and composition thereof |
| CN1785992A (en) * | 2005-12-13 | 2006-06-14 | 广州大学 | Method of extracting dibydro myricetrin from vine tea |
| CN1810798A (en) * | 2005-01-27 | 2006-08-02 | 广州大学 | Process of extracting dihydromyricitrin from vine tea |
-
2010
- 2010-04-16 CN CN2010101490141A patent/CN101805321B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712402A (en) * | 2004-06-25 | 2005-12-28 | 广东省农业科学院蚕业与农产品加工研究所 | Extraction of dihydromyricetin from microwave and composition thereof |
| CN1810798A (en) * | 2005-01-27 | 2006-08-02 | 广州大学 | Process of extracting dihydromyricitrin from vine tea |
| CN1785992A (en) * | 2005-12-13 | 2006-06-14 | 广州大学 | Method of extracting dibydro myricetrin from vine tea |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106931A (en) * | 2010-12-30 | 2011-06-29 | 张家界茅岩莓有限公司 | Method for producing diverse extracts of berry tea |
| CN102106931B (en) * | 2010-12-30 | 2013-03-20 | 张家界茅岩莓有限公司 | Method for producing diverse extracts of berry tea |
| CN102775455A (en) * | 2011-05-13 | 2012-11-14 | 贵州省生物研究所 | Dihydromyricetin medicine treating acute and chronic bronchitis and its preparation method |
| CN102584766A (en) * | 2011-12-31 | 2012-07-18 | 郁建平 | Method for simultaneously separating dihydromyricetin and myricetin from ampelopsis plant |
| CN102796069A (en) * | 2012-07-04 | 2012-11-28 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN102796069B (en) * | 2012-07-04 | 2015-01-21 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN104876903A (en) * | 2014-02-27 | 2015-09-02 | 天津药物研究院有限公司 | Crystallization form of dihydromyricetin, preparation method thereof and pharmaceutical composition containing the same |
| CN105010634A (en) * | 2015-07-06 | 2015-11-04 | 河南科技大学 | Moyeam tea cream and preparation method thereof |
| CN109053666A (en) * | 2018-09-05 | 2018-12-21 | 湖南中茂生物科技有限公司 | A kind of extracting method of dihydromyricetin |
| CN109349391A (en) * | 2018-12-20 | 2019-02-19 | 株洲千金药业股份有限公司 | A kind of preparation method and application of the long-lived vine tea of activity |
| CN113480508A (en) * | 2021-08-25 | 2021-10-08 | 武汉食味深山农业科技有限公司 | Extraction process of dihydromyricetin in vine tea and preparation process of vine tea functional beverage |
| CN113480508B (en) * | 2021-08-25 | 2022-04-22 | 武汉食味深山农业科技有限公司 | Extraction process of dihydromyricetin in vine tea and preparation process of vine tea functional beverage |
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| CN101805321B (en) | 2012-08-22 |
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Inventor after: Wei Yang Inventor after: Xiang Zhongju Inventor after: Chen Pengju Inventor after: Cheng Kejiong Inventor before: Wei Yang Inventor before: Xiang Zhongju Inventor before: Cai Ju Inventor before: Cheng Kejiong |
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Free format text: CORRECT: INVENTOR; FROM: WEI YANG XIANG ZHONGJU CAI JU CHENG KEJIONG TO: WEI YANG XIANG ZHONGJU CHEN PENGJU CHENG KEJIONG |
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