CN101804081A - Biological disinfectant and application thereof - Google Patents
Biological disinfectant and application thereof Download PDFInfo
- Publication number
- CN101804081A CN101804081A CN200910248908A CN200910248908A CN101804081A CN 101804081 A CN101804081 A CN 101804081A CN 200910248908 A CN200910248908 A CN 200910248908A CN 200910248908 A CN200910248908 A CN 200910248908A CN 101804081 A CN101804081 A CN 101804081A
- Authority
- CN
- China
- Prior art keywords
- biological disinfectant
- disinfectant
- culture medium
- biological
- enterococcus faecalis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a biological disinfectant, in particular to a biological disinfectant and application thereof. The biological disinfectant consists of candida utilis, lactobacillus acidophilus, enterococcus faecalis and bacillus subtilis. The raw materials are put into a culture medium until the pH value is between 2.5 and 3.5 and a supernate is centrifuged to obtain the biological disinfectant. The quantity ratio of the candida utilis to the lactobacillus acidophilus to the enterococcus faecalis to the bacillus subtilis is 1-3:1-3:1-3:1-3. The biological disinfectant can be used for livestocks and poultrys or be used for disinfecting the livestock and poultry housing. The biological disinfectant of the invention is safe and reliable and does not generate drug resistance and has green environment protection. The biological disinfectant not only is the disinfectant, but also is a nutrient substance of the animals, provides peptid nutriment and improves the animal immunity.
Description
Technical field
What the present invention relates to is microbial disinfectant, a kind of specifically biological disinfectant and application.
Background technology
Existing disinfectant is most to be the chemosynthesis disinfectant, life-time service causes the Drug resistance of microorganism, damage to the environment, use can cause in the house has in various degree infringement to the animal in raising, the part chemosterilant that uses in the animal body can cause dysbacteriosis in the animal body, immunity degradation, easily disease takes place, cause economic benefit to descend, to environment is produced considerable damage.
Summary of the invention
The object of the present invention is to provide a kind of safe, biological disinfectant and application of reliably, not producing drug resistance, environmental protection.
For achieving the above object, the technical solution used in the present invention is:
A kind of biological disinfectant: form by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis, then in culture medium, be cultured to pH value and reach 2.5-3.5, centrifuged supernatant, be biological disinfectant, the ratio of its Candida utilis, bacillus acidophilus, enterococcus faecalis, bacillus subtilis number of viable is for being 1-3: 1-3: 1-3: 1-3.
Described culture medium is agar 0.5～1.5g, Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g, defoamer 1.5～3.0g, peptone 0.5～1.5g, distilled water 1000mL.pH3.0～5.0。Described yeast extract is that yeast soaks powder.Described Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis are mixed after by mass percentage the inoculum concentration of 4-6% be inoculated in the culture medium in 35-37 ℃, fermentation culture 50-72 hour, make pH value reach 2.5-3.5, then with 15000 rev/mins of centrifugal 10-15 minutes, supernatant was biological disinfectant.
The application of biological disinfectant: described biological disinfectant can be used for being used for poultry, fowl or poultry, pouity dwelling place sterilization.
The advantage that the present invention had: biological disinfectant safety of the present invention, reliably, do not produce drug resistance, environmental protection, it is not only disinfectant simultaneously, and still the nutrient substance of animal provides peptide class nutrient, raising animal body immunity.
The specific embodiment
Embodiment 1
Biological disinfectant: will be 1: 1: 1 by the ratio of number of viable by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis: 1, then 4% inoculum concentration is inoculated in the culture medium in 37 ℃, fermentation culture 72 hours by mass percentage, then with 15000 rev/mins centrifugal 10 minutes, supernatant is biological disinfectant.
Described culture medium be agar 1g Carnis Bovis seu Bubali cream 1g, yeast extract 2.5g, sucrose 35g, defoamer (commercial product, present embodiment available from U.S. occasion limited company) 1.5g, peptone 1g, distilled water adds to 1000ml.Described yeast extract is that yeast soaks powder (commercial product, present embodiment yeast soak powder available from the extensive and profound in meaning star in Beijing Bioisystech Co., Ltd).
Described Candida utilis, enterococcus faecalis, bacillus acidophilus and bacillus subtilis carry out enrichment culture according to conventional method in following culture medium separately.
Described Candida utilis enrichment culture soaks powder (commercial) 3g in Rhizoma Solani tuber osi, and glucose 20g, agar powder 15g, distilled water add in the 1000ml culture medium, and 121 ℃ of autoclavings 20 minutes are standby.
Described enterococcus faecalis enrichment culture is in glucose 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.
Described bacillus acidophilus's enrichment culture is in glucose 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.Described bacillus subtilis enrichment culture is in peptone 10g, agar 20g, and sodium chloride 5g, meat soak powder 10g, and water adds to 1000ml, in the pH7.2 culture medium, 121 ℃ of autoclavings 20 minutes, standby.
Application examples:
With above-mentioned gained biological disinfectant to the escherichia coli in poultry, pouity dwelling place or the Aquatic product, the bactericidal effect of staphylococcus aureus is measured, get 100 times of above-mentioned biological disinfectant 100ml dilutions, act on escherichia coli, to staphylococcus aureus 10min, 15min, measure effect referring to table 1.This disinfectant is respectively 99.15% and 99.67% to the escherichia coli killing rate, is respectively 99.28% and 99.66% to the killing rate of staphylococcus aureus.
Table 1 disinfectant is to the killing effect of different microorganisms
Measure the stability of above-mentioned biological disinfectant simultaneously:
With biological disinfectant in the foregoing description, every bottle of 500ml places down 14d at 54 ℃ for totally 15 bottles, to the killing effect of escherichia coli with deposit preceding bactericidal effect similar (table 2).
Table 2 disinfectant stability test result
Embodiment 2
Biological disinfectant: will be 1: 2: 2 by the ratio of number of viable by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis: 1, then 6% inoculum concentration is inoculated in the culture medium in 35 ℃, fermentation culture 50 hours by mass percentage, then with 15000 rev/mins centrifugal 15 minutes, supernatant is biological disinfectant.Described culture medium is agar 0.5g, Carnis Bovis seu Bubali cream 0.5g, yeast extract 2g, sucrose 30g, defoamer 3.0g, peptone 0.5g, distilled water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.
Embodiment 3
Biological disinfectant: will be 3: 1: 1 by the ratio of number of viable by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis: 1, then 5% inoculum concentration is inoculated in the culture medium in 36 ℃, fermentation culture 60 hours by mass percentage, then with 15000 rev/mins centrifugal 12 minutes, supernatant is biological disinfectant.Described culture medium is agar 1.5g, Carnis Bovis seu Bubali cream 1.5g, yeast extract 2g, sucrose 45g, defoamer 2g, peptone 1.5g, distilled water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.
Embodiment 4
Biological disinfectant: will be 1: 2: 2 by the ratio of number of viable by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis: 1, then 4% inoculum concentration is inoculated in the culture medium in 37 ℃, fermentation culture 70 hours by mass percentage, then with 15000 rev/mins centrifugal 14 minutes, supernatant is biological disinfectant.Described culture medium is agar 1.2g, Carnis Bovis seu Bubali cream 1.3g, yeast extract 2.8g, sucrose 40g, defoamer 2g, peptone 0.8g, distilled water 1000mL.PH3.0～5.0,110～130 ℃ autoclaving.
Claims (4)
1. biological disinfectant, it is characterized in that: form by Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis, then in culture medium, be cultured to pH value and reach 2.5-3.5, centrifuged supernatant, be biological disinfectant, the ratio of its Candida utilis, bacillus acidophilus, enterococcus faecalis, bacillus subtilis number of viable is for being 1-3: 1-3: 1-3: 1-3.
2. by the described biological disinfectant of claim 1, it is characterized in that: described culture medium is agar 0.5～1.5g, Carnis Bovis seu Bubali cream 0.5～1.5g, yeast extract 2～3g, sucrose 30～45g, defoamer 1.5～3.0g, peptone 0.5～1.5g, distilled water 1000mL.pH3.0～5.0。
3. by the described biological disinfectant of claim 1, it is characterized in that: described Candida utilis, bacillus acidophilus, enterococcus faecalis and bacillus subtilis are mixed after by mass percentage the inoculum concentration of 4-6% be inoculated in the culture medium in 35-37 ℃, fermentation culture 50-72 hour, then with 15000 rev/mins of centrifugal 10-15 minutes, supernatant was biological disinfectant.
4. application by the described biological disinfectant of claim 1 is characterized in that: described biological disinfectant can be used for being used for poultry, fowl or poultry, pouity dwelling place sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910248908A CN101804081A (en) | 2009-12-30 | 2009-12-30 | Biological disinfectant and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910248908A CN101804081A (en) | 2009-12-30 | 2009-12-30 | Biological disinfectant and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101804081A true CN101804081A (en) | 2010-08-18 |
Family
ID=42606141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910248908A Pending CN101804081A (en) | 2009-12-30 | 2009-12-30 | Biological disinfectant and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101804081A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150689A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Complex microorganism disinfectant for vegetables |
| CN103053626A (en) * | 2013-01-07 | 2013-04-24 | 天津锦绣实业有限公司 | Natural lemon disinfectant and preparation method thereof |
| CN115948285A (en) * | 2022-11-23 | 2023-04-11 | 鲁东大学 | Biological disinfectant for lactobacillus salivarius BMC-06 and livestock breeding and application |
-
2009
- 2009-12-30 CN CN200910248908A patent/CN101804081A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150689A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Complex microorganism disinfectant for vegetables |
| CN102150689B (en) * | 2010-12-15 | 2013-07-24 | 沈阳科丰牧业科技有限公司 | Complex microorganism disinfectant for vegetables |
| CN103053626A (en) * | 2013-01-07 | 2013-04-24 | 天津锦绣实业有限公司 | Natural lemon disinfectant and preparation method thereof |
| CN115948285A (en) * | 2022-11-23 | 2023-04-11 | 鲁东大学 | Biological disinfectant for lactobacillus salivarius BMC-06 and livestock breeding and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103160455B (en) | Preparation method of spore preparation of bacillus coagulans | |
| CN106906154B (en) | Lactobacillus plantarum strain, feed additive and feed thereof | |
| CN103039699B (en) | Mixed fermentation feed and preparation method and application method of mixed fermentation feed | |
| CN103798540A (en) | Multi-strain microorganism pig feed additive and production method thereof | |
| CN101810248A (en) | Feed additive with complex microorganisms for piglets | |
| CN101199558A (en) | Compound probiotics for poultry and preparing method thereof | |
| CN105994941B (en) | A kind of nonreactive feed of microbial fermentation preparation | |
| CN104397323A (en) | Microbial feed additive | |
| CN101974463B (en) | Lactobacillus reuteri and composite viable bacteria preparation thereof | |
| CN101810247A (en) | Complex microbial chicken feed additive | |
| CN102524518A (en) | Method for producing antibacterial peptide by using brevibacillus laterosporu | |
| CN102660477A (en) | Lactobacillus brevis, freeze-dried powder of Lactobacillus brevis and application of freeze-dried powder | |
| CN106071261A (en) | A kind of probiotic additive promoting Poodle to digest and assimilate and preparation method thereof | |
| CN102517227B (en) | Enterococcus faecalis and applications and feed additive and leavening agent thereof | |
| CN101884394B (en) | Composite microbial feed additive for chicken and preparation method thereof | |
| CN104706678A (en) | Coated lactic acid bacterial microcapsule preparation method | |
| CN103289935A (en) | Compound strain microecological preparation and preparation method thereof | |
| CN103783321A (en) | Probiotic feed additive for Chinese softshell turtles and preparation method thereof | |
| KR101182754B1 (en) | Feed Additives Containing Microorganisms Cultured in Water Hyacinth Extract as Main Ingredient | |
| CN104351472A (en) | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth | |
| CN101804081A (en) | Biological disinfectant and application thereof | |
| CN104286419A (en) | Novel feed additive | |
| CN110241053A (en) | A kind of method of mixing fermentation culture clostridium butyricum | |
| CN103652329A (en) | Compound probiotic biological agent | |
| CN107981031A (en) | A kind of livestock and poultry environment-protecting feed and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100818 |