CN101798329A - Method for ultrasonic extraction of steviosides - Google Patents
Method for ultrasonic extraction of steviosides Download PDFInfo
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- CN101798329A CN101798329A CN 201010127458 CN201010127458A CN101798329A CN 101798329 A CN101798329 A CN 101798329A CN 201010127458 CN201010127458 CN 201010127458 CN 201010127458 A CN201010127458 A CN 201010127458A CN 101798329 A CN101798329 A CN 101798329A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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Abstract
The invention discloses a method for the ultrasonic extraction of steviosides. Smashed dry leaves of stevia rebaudiana serve as raw material; based on the characteristic that an ultrasonic cavitation can reinforce the extraction of active ingredients in plants, the solvent and the smashed raw materials flow in opposite directions, and the extract of the stevia rebaudiana is prepared by immersed extraction and ultrasonic extraction; white powder of the stevia rebaudiana is prepared by flocculating, filtering, absorbing, decolorizing, exchanging, concentrating, spray drying and the like; the stevioside content of the products is 85 to 98 percent. In the invention, the extraction time is only 10 to 60 minutes; the extraction rate can reach 95 to 99.9 percent; the temperature of the extraction solvent is only 10 to 40 DEG C; and the mass-to-volume ratio of the dry leaves to the solvent is only 1: 10-40 (kg: L). The method has the advantages of short extraction time, high extraction rate, low consumption of solvent, clean and sanitary production process and large scale and industrial continuous production; and the produced stevioside products have high purity and good quality.
Description
Technical field
The present invention relates to the method for a kind of extracting method of effective ingredients in plant, particularly a kind of ultrasonic extraction of steviosides.
Technical background
Steviosides, claim steviol glycoside again, be commonly called as stevioside, be the foodstuff additive of a kind of a kind of pure natural that from the blade of catananche's sweet Stevia, extracts, high sugariness, low heat value, edible safety, it is the third natural sugar source after sucrose, beet sugar, its sugariness is 200-300 a times of sucrose, but heat only is 1/300 of a sucrose.The Steviosides acid and alkali-resistance, to Heat stability is good, no fermentable, in the solution of pH3-9, can keep for a long time, also not decomposing, can be widely used among the sugared fields of nearly all usefulness such as food, beverage, wine brewing, medicine, essence and flavoring agent up to 200 ℃.Steviosides low heat value and edible safety, illnesss such as hypertension, hyperglycemia there is certain adjuvant treatment effect, it is edible to be suitable for specific crowds such as diabetes, hypertension, carious tooth, obesity, also is simultaneously pursuit health, advocates natural modern people's ideal sucrose substitute.
At present, operations such as Steviosides is drying, pulverizing, extraction, flocculation, filtration, absorption, decolouring, exchange mostly, concentrate, spraying drying make, in abstraction process, all adopt the mode of spacious cooking-pot type extraction tank (jar) to extract, promptly each sweet Stevia cured leaf with certain mass places groove (jar), the extraction solvent (as hot water) that adds 40-80 ℃ of temperature, start agitator and extract (also claiming extraction) glucoside, after waiting to stir the extraction certain hour, extracting solution is entered in the storage pool (jar).For glucoside is extracted fully, general operation is to add fresh extractor solvent again after having arranged extracting solution, repeats 4-8 time, and united extraction liquid enters next process and carries out purification, obtains the Steviosides finished product at last.In order to improve the utilization ratio of hot water, rare extracting solution that some producers also once extracted before the beginning several leading time (as 1-4 time) that feeds intake extracts as pure extraction solvent and obtains dense extracting solution and enter in the extracting solution storage pool (jar), and with back (as 5-8 time) rare extracting solution merging of obtaining with dew bright solvent extraction several times as the extracting solution of extraction next time.
The shortcoming of the production technique of the conventional extraction of steviosides of above-mentioned these is: extract the temperature height, reach 40-80 ℃, need consumes energy (as steam) many; Extraction time is many, as 4-8 time, can only operate in step mode; Extraction time is longer, needs 4-8 hour; In time do not extract because of the sweet Stevia melt cinder behind row's extracting solution, the glucoside loss is more, and the glucoside extraction yield is low, generally has only 80-90%; Solvent-oil ratio is big, and cured leaf and solvent ratio (quality) solvent ratio reaches 1: 40-50.Generally speaking, the extraction time of this conventional extraction of steviosides production technique is long, and extraction yield is low, and solvent-oil ratio is big, and energy consumption is big, and technical process is long, produces discontinuously, and occupation area of equipment is big, and level of automation is low.
In order to solve this present situation, Chinese patent CN101367852A discloses the method for high-efficiency and continuous extraction steviol glycoside in a kind of Folium Chrysanthemi.This method proposes the method that a kind of continuous countercurrent technique makes the steviol glycoside extracting solution, specifically be that Folium Chrysanthemi is by spiral way mode of motion extruding from top to down, solution (solvent) extracts steviol glycoside through Folium Chrysanthemi from bottom to top, solution and Folium Chrysanthemi remain the high density gradient, the residue of Folium Chrysanthemi is after the compressional movement on the screw, in the upper end steviol glycoside of Folium Chrysanthemi is extracted the back and become raw sugar liquid, be recovered in the lower end.This method makes sense theoretically, but in fact have the following disadvantages: (1) Folium Chrysanthemi is difficult to be stirred smoothly by spiral way in extracting solvent without pulverizing, so solution and Folium Chrysanthemi are difficult to remain the high density gradient; (2) though adopt reflux type, can keep a higher concentration gradient, because of not disposing other assisted extraction means such as ultrasonic wave, the dissolution rate of glucoside composition is slower, so extraction time is still longer relatively; (3), extract device length and can become very long, so floor space and facility investment all can be bigger in order to keep higher glucoside extraction yield; (4) this method does not dispose assisted extraction means such as ultrasonic wave in leaching process, extraction efficiency is lower, the extraction yield of steviol glycoside is about 90%, though more traditional common process increase, but 10% the glucoside of still having an appointment does not extract and enters in the extracting solution and cause waste.
Summary of the invention
In order to solve the above-mentioned deficiency that has extraction of steviosides method from the sweet Stevia cured leaf now, the present invention designed a kind of from the sweet Stevia cured leaf method of extraction of steviosides, this method is a raw material with the sweet Stevia cured leaf of pulverizing, utilize the characteristics of the extraction of effective constituent in the cavitation effect of ultrasonic waves energy fortification of plants, solvent and pulverizing raw material flow direction be adverse current each other, adopt continuous countercurrent supersound extraction unit to carry out the extraction of Steviosides composition, overcome the deficiencies in the prior art.
The theoretical foundation of this method is according to the ultrasonic cavitation effect.Ultrasonic wave is meant the elastic wave that the higher object of vibrational frequency is produced in medium, its range of frequency is 20kHz-10MHz.The mechanism of action of ultrasonic wave and medium mainly is a cavatition.In liquid, when the power of sound wave is quite big, liquid is subjected to negative pressure enough by force the time, the intermolecular mean distance of the medium distance that will increase and overstep the extreme limit, thereby liquid is broken the formation hole, in fierce contraction of the cavity of cavitation bubble or cavitation and the moment of collapsing, can produce partial high pressure in the bubble, and the high temperature of thousands of degree, produce microjet and TRANSIENT HIGH TEMPERATURE high pressure, thereby form ultrasonic cavitation.Cavitation phenomenon comprises formation, growth and the collapse process of bubble.Cavatition is the active force of sonochemistry, and the particle movement velocity is accelerated greatly, destroys the formation of the power of particle, thereby makes many physical chemistry and chemical process violent acceleration, and emulsification, dispersion, extraction and other various technological processs are had great role.The velocity of diffusion of effective constituent is quickened in formed frictional belt when the cavatition that ultrasonic wave produces can effectively be broken effective constituent stripping solid, thereby improves extraction rate.The leaching process of effective ingredients in plant is a liquid-solid extraction process in essence, and concentration difference size and boundary layer thickness that effective constituent is extracted the velocity of diffusion of solvent and solid surface and peripheral solvent from the plant cell wall stripping are closely related.Except that cavatition, hyperacoustic many second-order effects also all help the transfer and the extraction of active ingredients of plants, as mechanical vibration, disturbance effect, high acceleration, emulsification, spread, smash and multistage effects such as stirring action, can effectively increase molecular motion of material frequency and speed, increase the solvent penetration power, improve solid-liquid and liquid-liquid mass transfer rate of diffusion, enter solvent thereby quicken effective ingredient, shorten extraction time, effectively improve extraction yield.
Technical scheme of the present invention is:
Sweet Stevia cured leaf with pulverizing is a raw material; Raw material is imported from extraction tube one end, extracting solvent imports from the extraction tube the other end, to raw material and the continuous helical stir of extraction solvent, realize soaking and extract, again under ultrasonic environment to raw material with extract the continuous helical stir of solvent, realize supersound extraction, push the stevioside leaf slag then, the realization melt cinder separates, and makes the sweet Stevia extracting solution; The sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make the white powder product, the Steviosides content 85-98% of product; Carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag.
The sweet Stevia cured leaf of pulverizing is the 5-40 order, preferred 10-25 order.
The extraction temperature of extracting solvent is 10-40 ℃, preferred 20-40 ℃.
The extraction solvent is the aqueous solution of water, enzyme, one or more and combination thereof in the organic solvent (as sherwood oil, ethanol), the aqueous solution of preferably water, enzyme.
Extraction time is 10-60 minute, preferred 20-50 minute.
The mass volume ratio of sweet Stevia cured leaf and solvent is 1: and 10-40 (kg: L), preferred 1: 20-40 (kg: L).
Described is the ultrasonic generator generation of 20-200kw at ultrasonic environment by power, and the preferred power of ultrasonic generator is 50-120kw.
Organic solvent is a kind of or its combination in sherwood oil, the ethanol.
Extraction tube disposes the on-line cleaning device, can carry out on-line cleaning to extraction tube, meets the hygienic requirements of GMP authentication to equipment.
Adopt the beneficial effect of method of the present invention to be, the extraction time of Steviosides composition only needs 10-60 minute, extraction yield can reach 95-99.9%, extracting solvent temperature is 10-40 ℃, cured leaf and solvent quality volume ratio are 1: 10-40 (kg: L), article one, extraction production line day charging capacity is 1-100t, has realized the industrial high efficiency continuous production of Steviosides composition ultrasonic extraction.Adopt method of the present invention, Steviosides composition leaching process extraction time is short, the extraction yield height, and it is low to extract temperature, and solvent consumption is few, and the production process sanitation and hygiene can the large-scale industrial continuous production, and the Steviosides product purity height of production, quality are good.
Embodiment
Below in conjunction with embodiment the present invention is done and to describe in further detail.
Examples of implementation 1
A kind of method of ultrasonic extraction of steviosides, its steps in sequence is:
(a) be raw material to be ground into 10 purpose sweet Stevia cured leafs;
(b) adopt the ultrasonic Steviosides composition that carries out of continuous countercurrent to extract.To be ground into 10 purpose sweet Stevia cured leaf 200kg with feeding unit quantitatively imports from an end of extraction tube, quantitatively adding temperature from the other end of extraction tube with feeding unit is that 20 ℃ aqueous solution 4000L is as extracting solvent, start helical stir and ultrasonic generator, ultrasonic generator power is 60kw, the sweet Stevia cured leaf of helical stir extract through 25 minutes immersion and 25 minutes supersound extraction after, realize that through extruding melt cinder separates, carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag, make sweet Stevia extracting solution 4000L.
(c) the sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make white Steviosides powder 20kg, product Steviosides content 88%.
Examples of implementation 2
A kind of method of ultrasonic extraction of steviosides, its steps in sequence is:
(a) be raw material to be ground into 14 purpose sweet Stevia cured leafs;
(b) adopt the ultrasonic Steviosides composition that carries out of continuous countercurrent to extract.To be ground into 14 purpose sweet Stevia cured leaf 500kg with feeding unit quantitatively imports from an end of extraction tube, quantitatively adding temperature from the other end of extraction tube with feeding unit is that 25 ℃ aqueous solution 12500L is as extracting solvent, start helical stir and ultrasonic generator, ultrasonic generator power is 80kw, the sweet Stevia cured leaf of helical stir extract through 21 minutes immersion and 21 minutes supersound extraction after, realize that through extruding melt cinder separates, carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag, make sweet Stevia extracting solution 10000L.
(c) the sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make white Steviosides powder 50kg, product Steviosides content 92%.
Examples of implementation 3
A kind of method of ultrasonic extraction of steviosides, its steps in sequence is:
(a) be raw material to be ground into 20 purpose sweet Stevia cured leafs;
(b) adopt the ultrasonic Steviosides composition that carries out of continuous countercurrent to extract.To be ground into 20 purpose sweet Stevia cured leaf 1000kg with feeding unit quantitatively imports from an end of extraction tube, quantitatively adding temperature from the other end of extraction tube with feeding unit is that 30 ℃ aqueous solution 30000L is as extracting solvent, start helical stir and ultrasonic generator, ultrasonic generator power is 100kw, the sweet Stevia cured leaf of helical stir extract through 17 minutes immersion and 17 minutes supersound extraction after, realize that through extruding melt cinder separates, carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag, make sweet Stevia extracting solution 30000L.
(c) the sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make white Steviosides powder 100kg, product Steviosides content 97%.
Examples of implementation 4
A kind of method of ultrasonic extraction of steviosides, its steps in sequence is:
(a) be raw material to be ground into 25 purpose sweet Stevia cured leafs;
(b) adopt the ultrasonic Steviosides composition that carries out of continuous countercurrent to extract.To be ground into 25 purpose sweet Stevia cured leaf 1500kg with feeding unit quantitatively imports from an end of extraction tube, quantitatively adding temperature from the other end of extraction tube with feeding unit is that 35 ℃ aqueous solution 52500L is as extracting solvent, start helical stir and ultrasonic generator, ultrasonic generator power is 120kw, the sweet Stevia cured leaf of helical stir extract through 14 minutes immersion and 14 minutes supersound extraction after, realize that through extruding melt cinder separates, carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag, make sweet Stevia extracting solution 52500L.
(c) the sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make white Steviosides powder 150kg, product Steviosides content 95%.
Examples of implementation 5
A kind of method of ultrasonic extraction of steviosides, its steps in sequence is:
(a) be raw material to be ground into 40 purpose sweet Stevia cured leafs;
(b) adopt the ultrasonic Steviosides composition that carries out of continuous countercurrent to extract.To be ground into 25 purpose sweet Stevia cured leaf 3000kg with feeding unit quantitatively imports from an end of extraction tube, quantitatively adding temperature from the other end of extraction tube with feeding unit is that 40 ℃ aqueous solution 120000L is as extracting solvent, start helical stir and ultrasonic generator, ultrasonic generator power is 150kw, the sweet Stevia cured leaf of helical stir extract through 10 minutes immersion and 10 minutes supersound extraction after, realize that through extruding melt cinder separates, carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag, make sweet Stevia extracting solution 120000L.
(c) the sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make white Steviosides powder 300kg, product Steviosides content 91%.
Claims (8)
1. the method for a ultrasonic extraction of steviosides is characterized in that: with the sweet Stevia cured leaf pulverized is raw material; Raw material is imported from extraction tube one end, extracting solvent imports from the extraction tube the other end, to raw material and the continuous helical stir of extraction solvent, realize soaking and extract, again under ultrasonic environment to raw material with extract the continuous helical stir of solvent, realize supersound extraction, push the stevioside leaf slag then, the realization melt cinder separates, and makes the sweet Stevia extracting solution; The sweet Stevia extracting solution through flocculation, filter, absorption, decolouring, exchange, concentrate, after the operation such as spraying drying, make the white powder product, the Steviosides content 85-98% of product; Carry out secondary immersion extraction and supersound extraction through pushing isolated stevioside leaf slag.
2. the method for a kind of ultrasonic extraction of steviosides according to claim 1, it is characterized in that: the sweet Stevia cured leaf of pulverizing is the 5-40 order, preferred 10-25 order.
3. according to the method for the described a kind of ultrasonic extraction of steviosides of claim, it is characterized in that: the extraction temperature of extracting solvent is 10-40 ℃, preferred 20-40 ℃.
4. the method for a kind of ultrasonic extraction of steviosides according to claim 1 is characterized in that: the extraction solvent is the aqueous solution of water, enzyme, one or more and combination thereof in the organic solvent, the aqueous solution of preferably water, enzyme.
5. according to the method for the described a kind of ultrasonic extraction of steviosides of claim, it is characterized in that: the time of soaking extraction and supersound extraction is 10-60 minute, preferred 20-50 minute.
6. the method for a kind of ultrasonic extraction of steviosides according to claim 1, it is characterized in that: the mass volume ratio of sweet Stevia cured leaf and solvent is 1: 10-40 (kg: L), preferred 1: 20-40 (kg: L).
7. the method for a kind of ultrasonic extraction of steviosides according to claim 1 is characterized in that: described is that the ultrasonic generator of 20-200kw produces at ultrasonic environment by power, and the preferred power of ultrasonic generator is 50-120kw.
8. the method for a kind of ultrasonic extraction of steviosides according to claim 4, it is characterized in that: organic solvent is a kind of or its combination in sherwood oil, the ethanol.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010127458 CN101798329A (en) | 2010-03-19 | 2010-03-19 | Method for ultrasonic extraction of steviosides |
| PCT/CN2011/071965 WO2011113373A1 (en) | 2010-03-19 | 2011-03-18 | Method for ultrasonic extraction of stevioside |
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| CN 201010127458 CN101798329A (en) | 2010-03-19 | 2010-03-19 | Method for ultrasonic extraction of steviosides |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102001885A (en) * | 2010-10-28 | 2011-04-06 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN102127129A (en) * | 2010-12-13 | 2011-07-20 | 辽宁千千生物科技有限公司 | Method for extracting stevioside from fresh stevia rebaudiana leaves |
| WO2011113373A1 (en) * | 2010-03-19 | 2011-09-22 | 赣州菊隆高科技实业有限公司 | Method for ultrasonic extraction of stevioside |
| CN102199177A (en) * | 2011-03-23 | 2011-09-28 | 武汉普赛特膜技术循环利用有限公司 | Manufacturing method of pure natural high-purity stevioside |
| CN102250990A (en) * | 2011-05-31 | 2011-11-23 | 江南大学 | Method for preparing rubusoside by catalytically hydrolyzing stevioside with beta-galactosidase |
| CN102775452A (en) * | 2012-06-14 | 2012-11-14 | 甘肃普华甜菊糖开发有限公司 | Stevioside purification process |
| US20130164434A1 (en) * | 2011-12-16 | 2013-06-27 | Almendra Pte Ltd. | Process for the Purification of Rebaudioside A and Compositions Thereof |
| WO2014097319A1 (en) * | 2012-12-22 | 2014-06-26 | Sevak Niravkumar Prakashbhai | Extraction method for organic certifiable steviosides from organic stevia leaves |
| CN105294790A (en) * | 2015-12-07 | 2016-02-03 | 湖南华诚生物资源股份有限公司 | Method for extracting high-purity steviol glycosides from stevia rebaudiana |
| CN106146574A (en) * | 2016-06-17 | 2016-11-23 | 蚌埠市华东生物科技有限公司 | A kind of method of stevioside in microwave ultrasound compound collecting leaf of Folium Stevlae Rebaudianae |
| CN106831906A (en) * | 2017-01-13 | 2017-06-13 | 西安天丰生物科技有限公司 | A kind of ultrasonic wave added method extraction of steviosides from STEVIA REBAUDIANA |
| CN109265346A (en) * | 2018-09-30 | 2019-01-25 | 晨光生物科技集团股份有限公司 | The industrialized utilization method and its chlorogenic acid and steviol glycoside of a kind of STEVIA REBAUDIANA |
| CN109293712A (en) * | 2018-09-30 | 2019-02-01 | 晨光生物科技集团股份有限公司 | The industrialized utilization method and its steviol glycoside and chlorogenic acid of a kind of STEVIA REBAUDIANA |
| CN110192585A (en) * | 2019-06-19 | 2019-09-03 | 蚌埠学院 | A kind of Lu`an Guapian.Tea lily milk tea and preparation method thereof |
| CN114984613A (en) * | 2022-05-30 | 2022-09-02 | 青岛安美瑞生物科技产业园股份有限公司 | Plant polysaccharide extraction and drying process and device for implementing process |
| CN115946387A (en) * | 2022-08-31 | 2023-04-11 | 山东圣香远生物科技股份有限公司 | Extraction method and application of high-activity stevioside |
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| CN101367852A (en) * | 2008-09-09 | 2009-02-18 | 王德骥 | High-efficiency method for continuously extracting stevioside from stevia leaf |
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| CN101798329A (en) * | 2010-03-19 | 2010-08-11 | 赣州菊隆高科技实业有限公司 | Method for ultrasonic extraction of steviosides |
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| JPS51131462A (en) * | 1975-05-13 | 1976-11-15 | Toyo Ink Mfg Co Ltd | An extraction method |
| CN101367852A (en) * | 2008-09-09 | 2009-02-18 | 王德骥 | High-efficiency method for continuously extracting stevioside from stevia leaf |
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011113373A1 (en) * | 2010-03-19 | 2011-09-22 | 赣州菊隆高科技实业有限公司 | Method for ultrasonic extraction of stevioside |
| CN102001885B (en) * | 2010-10-28 | 2013-07-03 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN102001885A (en) * | 2010-10-28 | 2011-04-06 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN102127129A (en) * | 2010-12-13 | 2011-07-20 | 辽宁千千生物科技有限公司 | Method for extracting stevioside from fresh stevia rebaudiana leaves |
| CN102199177A (en) * | 2011-03-23 | 2011-09-28 | 武汉普赛特膜技术循环利用有限公司 | Manufacturing method of pure natural high-purity stevioside |
| CN102250990A (en) * | 2011-05-31 | 2011-11-23 | 江南大学 | Method for preparing rubusoside by catalytically hydrolyzing stevioside with beta-galactosidase |
| US9994606B2 (en) * | 2011-12-16 | 2018-06-12 | Almendra Pte Ltd. | Process for the purification of Rebaudioside A and compositions thereof |
| US20130164434A1 (en) * | 2011-12-16 | 2013-06-27 | Almendra Pte Ltd. | Process for the Purification of Rebaudioside A and Compositions Thereof |
| CN102775452A (en) * | 2012-06-14 | 2012-11-14 | 甘肃普华甜菊糖开发有限公司 | Stevioside purification process |
| WO2014097319A1 (en) * | 2012-12-22 | 2014-06-26 | Sevak Niravkumar Prakashbhai | Extraction method for organic certifiable steviosides from organic stevia leaves |
| CN105294790A (en) * | 2015-12-07 | 2016-02-03 | 湖南华诚生物资源股份有限公司 | Method for extracting high-purity steviol glycosides from stevia rebaudiana |
| CN105294790B (en) * | 2015-12-07 | 2018-11-16 | 湖南华诚生物资源股份有限公司 | A method of extracting high-purity stevioside from STEVIA REBAUDIANA |
| CN106146574A (en) * | 2016-06-17 | 2016-11-23 | 蚌埠市华东生物科技有限公司 | A kind of method of stevioside in microwave ultrasound compound collecting leaf of Folium Stevlae Rebaudianae |
| CN106831906A (en) * | 2017-01-13 | 2017-06-13 | 西安天丰生物科技有限公司 | A kind of ultrasonic wave added method extraction of steviosides from STEVIA REBAUDIANA |
| CN109293712A (en) * | 2018-09-30 | 2019-02-01 | 晨光生物科技集团股份有限公司 | The industrialized utilization method and its steviol glycoside and chlorogenic acid of a kind of STEVIA REBAUDIANA |
| CN109265346A (en) * | 2018-09-30 | 2019-01-25 | 晨光生物科技集团股份有限公司 | The industrialized utilization method and its chlorogenic acid and steviol glycoside of a kind of STEVIA REBAUDIANA |
| WO2020063894A1 (en) * | 2018-09-30 | 2020-04-02 | 晨光生物科技集团股份有限公司 | Industrial utilization method for stevia rebaudiana and stevioside and chlorogenic acid of stevia rebaudiana |
| KR20210076030A (en) * | 2018-09-30 | 2021-06-23 | 첸광 바이오테크 그룹 캄파니 리미티드 | Industrial use method of Stevia rebaudiana and its stevioside and chlorogenic acid |
| CN109265346B (en) * | 2018-09-30 | 2022-07-01 | 晨光生物科技集团股份有限公司 | Industrialized utilization method of stevia rebaudiana and chlorogenic acid and stevioside thereof |
| KR102600268B1 (en) | 2018-09-30 | 2023-11-10 | 첸광 바이오테크 그룹 캄파니 리미티드 | Industrial use method of Stevia rebaudiana and its stevioside and chlorogenic acid |
| US11891411B2 (en) | 2018-09-30 | 2024-02-06 | Chenguang Biotech Group Co., Ltd. | Industrial utilization method for Stevia rebaudiana and stevioside and chlorogenic acid of Stevia rebaudiana |
| CN110192585A (en) * | 2019-06-19 | 2019-09-03 | 蚌埠学院 | A kind of Lu`an Guapian.Tea lily milk tea and preparation method thereof |
| CN114984613A (en) * | 2022-05-30 | 2022-09-02 | 青岛安美瑞生物科技产业园股份有限公司 | Plant polysaccharide extraction and drying process and device for implementing process |
| CN115946387A (en) * | 2022-08-31 | 2023-04-11 | 山东圣香远生物科技股份有限公司 | Extraction method and application of high-activity stevioside |
| CN115946387B (en) * | 2022-08-31 | 2023-09-15 | 山东圣香远生物科技股份有限公司 | High-activity stevioside extraction method and application thereof |
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