CN101768546B - Peristaltic solid-state fermentation technology and device - Google Patents
Peristaltic solid-state fermentation technology and device Download PDFInfo
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- CN101768546B CN101768546B CN 201010113731 CN201010113731A CN101768546B CN 101768546 B CN101768546 B CN 101768546B CN 201010113731 CN201010113731 CN 201010113731 CN 201010113731 A CN201010113731 A CN 201010113731A CN 101768546 B CN101768546 B CN 101768546B
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- air bag
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
Abstract
The invention discloses a peristaltic solid-state fermentation technology and device for traditional Chinese medicine. The technical scheme is to lay 2-8 silicone rubber gasbags at the bottom of a fermenter, wherein, the gasbags are presented Tai Chi shape, U-shape, M-shape, semicircle shape or bar shape; on the gasbags, a bubble prominence is arranged; the gasbags are connected with a compressor to periodically open or close a solenoid valve through a controller; the gasbags go up or down during the solid-state fermentation of the traditional Chinese medicine, thereby realizing a periodic peristalsis stimulation, playing a role in mixing, loosening and resisting agglomeration of the traditional Chinese medicine agglomeration during solid-state fermentation, strengthening the heat and mass transfer during solid-state fermentation, shortening the fermentation period, and improving the fermentation efficiency and the yield.
Description
Technical field
The invention belongs to Chinese medicine solid state fermentation research field, specially refer to a kind of peristaltic solid-state fermentation technology and device.
Background technology
The Chinese medicine solid state fermentation is derived from ancient times " yeast-making technology ", and modern age, production technique had certain development.In solid state fermentation, zymophyte is to grow under the situation near natural condition, might produce the meta-bolites that some do not produce in liquid culture, can make the Chinese medicine Divine Comedy such as only having by solid state fermentation.But, the Chinese medicine solid state fermentation is normally static, material easily lumps in static solid ferment process, mycelia is only in surface growth, be difficult to go deep into fermented substrate inside, solid state fermentation mass-and heat-transfer efficient is low simultaneously, and fermentation period is long, equipment fails to realize mechanize and automatization, and these problems have limited Chinese medicine solid state fermentation industry development process.Although mechanical stirring is the effective means of enhancing mass and heat transfer normally, but in the Chinese medicine solid-state fermentation process, the fermented bacterium overwhelming majority is medicinal fungi, the shearing force that mechanical stirring causes often causes the mycelia fracture, greatly affect the growth of medicinal fungi, so the Chinese medicine solid state fermentation is so far still take static fermentation as main.
Chinese invention patent ZL 200610017078.X provides a kind of creeping motion type fermentor tank that can be used for solid state substrate, but there is certain limitation in it: the supporting walls inner chamber can not hold fermentation materials, and the whole reality that has reduced fermentor tank can be utilized volume; As motivating force, if the fluid pipeline leakage easily causes the tank body conduction, there is potential safety hazard with piezoelectric patches and spring; Structure is too complicated, and manufacturing cost is higher, is unfavorable for the engineering amplification; The content of herb fermenting stroma starch, grease and protein is higher, only relies on the wriggling of flexible wall can't effectively avoid the caking of Chinese medicine matrix in solid ferment process.
The present invention is inspired from gastrointestinal peristalsis, by bionic theory, the Chinese medicine solid state fermentation is introduced in the creeping motion type fermentation, realize dynamic solid ferment process with normal force, both strengthened mass-and heat-transfer, fundamentally avoided again shearing force that traditional mechanical stirring brings to the disadvantageous effect of thalline, overturned for a long time Chinese medicine solid state fermentation mode static and mechanical agitation type, a kind of Chinese medicine solid-state fermentation technology and device of novel creeping motion type is provided.
Summary of the invention
Problem to be solved by this invention provides a kind of fermentation technique and device, is particularly useful for a kind of creeping motion type fermentation technique and the device of Chinese medicine solid state fermentation.
The present invention adopts following technical scheme that a kind of peristaltic solid-state fermentation technology and device are provided, its substantive distinguishing features is to lay 2-8 air bag in the fermentor tank bottom, be pressed into air by compressor cycle, air bag is along with harmomegathus, in the Chinese medicine solid ferment process, realize creeping motion type period stimulating, the mass-and heat-transfer in the forced fermentation process by controller.
According to a kind of peristaltic solid-state fermentation technology and the device that technical scheme provides, its substantive distinguishing features is that the material of described air bag is silicon rubber, and one side is bonded at fermentor tank bottom, another side distribution blister projection.
A kind of peristaltic solid-state fermentation technology and the device that provide according to technical scheme, the ratio of height was 1: 5～1: 20 when its substantive distinguishing features was described blister rising height and air bag bulging, and the ratio of the area of blister projection and air bag area is 1: 10～1: 100.
According to a kind of peristaltic solid-state fermentation technology and the device that technical scheme provides, its substantive distinguishing features is that described fermentor tank bottom laying air bag is Diagram of the Supreme Ultimate shape, U-shaped, M shape, semicircle or bar shaped.
According to a kind of peristaltic solid-state fermentation technology and the device that technical scheme provides, its substantive distinguishing features is that described air bag is pressed into air by compressor or water reaches expansion.
According to a kind of peristaltic solid-state fermentation technology and the device that technical scheme provides, its substantive distinguishing features is that described controller is regulated compressor and electromagnetic valve switch, thus control air bag periodicity harmomegathus.
A kind of peristaltic solid-state fermentation technology provided by the invention and device, its advantage is:
1. air bag is laid on the fermentor tank bottom, and the tank body volume is utilized effectively;
2. by the periodic peristaltic of fermentor tank bottom air bag, can play mixing, loosen, prevent the effect that material lumps for the Chinese medicine in the solid ferment process, strengthen the Chinese medicine solid state fermentation by period stimulating;
3. can change air bag and lay number and angle by adjusting the balloon surface structure, to the different periodic peristaltic hormesis of solid state fermentation performance of different Chinese medicine materials;
4. simple in structure, cost is lower, is conducive to equipment and amplifies.
Description of drawings
Fig. 1 is the front view of a kind of peristaltic solid-state fermentation technology and apparatus structure principle sketch;
Fig. 2 is the vertical view of a kind of peristaltic solid-state fermentation technology and apparatus structure principle sketch;
Fig. 3 is the side-view of a kind of peristaltic solid-state fermentation technology and apparatus structure principle sketch;
Fig. 4 is the vertical view of a kind of four gasbags peristaltic solid-state fermentation technology and apparatus structure principle sketch;
Among the figure: 1. air bag, 2. air bag, 3. magnetic valve, 4. magnetic valve, 5. fermentor tank, 6. opening for feed.
Embodiment
The present invention will be further described below in conjunction with the drawings and specific embodiments.
450g Radix Astragali meal is mixed with the 50g wheat bran, add 500ml distilled water, 121 ℃ of steam sterilizing 30min; the Phellinus bacterial classification of access shake-flask culture places creeping motion type fermentor tank 5 after the sterilization by opening for feed 6, opens air compressor; by controller and magnetic valve 3,4, be filled with air to air bag 1, keep 1 venting of 30s rear gasbag; simultaneously to air bag 2 inflations; keep 30s after being full of, then venting is again to air bag 1 inflation; so move in circles, until fermentation ends.
Fermentation materials is dry, extract and obtain Crude polysaccharides 25.54g, to compare static fermentation period and shorten 10 days, polysaccharide yield improves 8.42%.
The present embodiment adopts a kind of four gasbags Chinese medicine creeping motion type solid-state fermentation installation shown in Figure 4.
Take by weighing 500g Radix Astragali meal, add 500ml distilled water, 121 ℃ of steam sterilizing 30min, the Ganderma lucidum of access shake-flask culture places creeping motion type fermentor tank after the sterilization by opening for feed, opens water pump, by controller and magnetic valve, water-filling in one of them air bag, keep swelling state 3min after, water is injected next air bag, keep 3min, water is injected next air bag, dilation so successively is until fermentation ends again.
Fermentation materials is dry, extract and obtain Crude polysaccharides 27.63g.
Claims (4)
1. Chinese medicine creeping motion type solid-state fermentation installation, by fermentor tank (5), air bag (1,2), compressor or water pump and controller form, it is characterized in that, air bag (1,2) one side is bonded at the fermentor tank bottom, another side distribution blister projection is laid on fermentor tank (5) bottom, the ratio of its height of blister projection time height with the air bag bulging is 1: 5～1: 20, the ratio of the area of blister projection and air bag area is 1: 10～1: 100, the air bag number is 2-8, compressor or water pump and air bag (1,2) pass through pipeline communication, magnetic valve (3 is established in the pipeline stage casing, 4), controller connected electromagnetic valve (3,4) control air or water turnover air bag (1,2), in the Chinese medicine solid ferment process, open compressor or water pump, by controller electromagnetic valve for adjusting (3,4) switch, air bag (1,2) cycle replaces bulging and contraction.
2. Chinese medicine creeping motion type solid-state fermentation installation according to claim 1 is characterized in that, the material of air bag is silicon rubber.
3. Chinese medicine creeping motion type solid-state fermentation installation according to claim 1, the described air bag that is laid on the fermentor tank bottom, its laying be shaped as Diagram of the Supreme Ultimate shape, U-shaped, M shape, semicircle or bar shaped.
4. adopt Chinese medicine creeping motion type solid-state fermentation installation claimed in claim 1 to carry out the method for Chinese medicine creeping motion type solid state fermentation, it is characterized in that, the Chinese medicine disinfection inoculation is placed in the Chinese medicine creeping motion type solid-state fermentation installation, open air compressor or water pump, agitate the air bag of fermentor tank bottom, behind the maintenance 10-300s, venting or draining, after air bag shrinks, refill air or water, so move in circles.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010113731 CN101768546B (en) | 2010-02-24 | 2010-02-24 | Peristaltic solid-state fermentation technology and device |
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| CN 201010113731 CN101768546B (en) | 2010-02-24 | 2010-02-24 | Peristaltic solid-state fermentation technology and device |
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| CN101768546A CN101768546A (en) | 2010-07-07 |
| CN101768546B true CN101768546B (en) | 2013-01-23 |
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| CN 201010113731 Expired - Fee Related CN101768546B (en) | 2010-02-24 | 2010-02-24 | Peristaltic solid-state fermentation technology and device |
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| CN106718971B (en) * | 2016-11-24 | 2020-10-16 | 新昌县兴欧智能科技有限公司 | Ecological cattle raising fermentation bed |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1526812A (en) * | 2003-09-23 | 2004-09-08 | 西北农林科技大学 | Large-scale movable laterally ventilating thick-layer fermentation apparatus and process |
| WO2005012478A1 (en) * | 2003-07-31 | 2005-02-10 | Prophyta Biologischer Pflanzenschutz Gmbh | Solid-state fermenter |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
| CN201206141Y (en) * | 2008-04-02 | 2009-03-11 | 朱金华 | Solid material fermentation tank |
-
2010
- 2010-02-24 CN CN 201010113731 patent/CN101768546B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005012478A1 (en) * | 2003-07-31 | 2005-02-10 | Prophyta Biologischer Pflanzenschutz Gmbh | Solid-state fermenter |
| CN1526812A (en) * | 2003-09-23 | 2004-09-08 | 西北农林科技大学 | Large-scale movable laterally ventilating thick-layer fermentation apparatus and process |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
| CN201206141Y (en) * | 2008-04-02 | 2009-03-11 | 朱金华 | Solid material fermentation tank |
Non-Patent Citations (2)
| Title |
|---|
| 廖春燕，郑裕国.固态发酵生物反应器.《微生物学通报》.2005,第32卷(第1期),99-103. * |
| 李宏强, 陈洪章.固态发酵的参数周期变化及对微生物发酵的影响.《生物工程学报》.2005,第21卷(第3期),440-445. * |
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