CN101747421B

CN101747421B - Method for extracting soybean protein through taking fresh stem leaf of soybean as raw material

Info

Publication number: CN101747421B
Application number: CN2010101006238A
Authority: CN
Inventors: 于海
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-01-22
Filing date: 2010-01-22
Publication date: 2012-07-04
Anticipated expiration: 2030-01-22
Also published as: CN101747421A

Abstract

The invention discloses a method for extracting soybean protein through taking the fresh stem leaf of soybean as raw material, which comprises the following steps: collecting the fresh stem leaves of soybean plant in the early flowering, cleaning the leaves with water, beating and squeezing the fresh stem leaves, filtering the impurities, heating, filtering or fermenting, protein is concreted after being heated, and filtering out the precipitated protein; or adopting the fermentation method, utilizing the lactic acid produced by lactic acid bacillus to make the protein in the juice concrete and precipitate, and then filtering the precipitated protein; heating to deodorize, and separating the precipitated protein into wet cake with water content of 60 percent; and then drying, sterilizing and packaging the protein. The method is that protein is extracted from fresh stem leaf and the stem leaf is collected in the early flowering, in the North of China, the places on which only first crop soybeans are planted originally, but secondary or third crop soybeans can be planted, so higher biological yield per unit area can be realized, thereby obtaining higher yield of plant crude protein per unit area. The crude protein powder product extracted by the method also contains multiple minerals and microelements, and can be the food providing plant protein and other nutrients.

Description

With the bright cauline leaf of soybean is that raw material extracts soy proteinaceous method

It is the raw material method for extracting proteins that technical field the present invention relates to a kind of cauline leaf with bean, be specifically a kind of be the raw material method for extracting proteins with the bright cauline leaf of soybean, black soya bean.

Background technology for a long time, soybean meal is to produce proteinic main raw material always.Because domestic dregs of beans resource is few, and major part is used for the feed industry, causes that soy-protein quantity is few on the market, price is high.

It is higher that the soybean cauline leaf contains protein; Research according to academy of agricultural sciences, the Shanxi Province variety of crops The Study on Resources Li Ying researcher of institute is measured; The soybean blade nitrogenous 4%-6% in early stage that bears pods, the nitrogenous 2.5%-3.5% of stem (" black soya bean plantation and processing and utilization "--Golden Shield press in March, 2002).The nitrogen content of soybean cauline leaf multiply by 6.25 coefficients, and (6.25 coefficients are the coefficient of general measuring and calculating leguminous plants of world feeler mechanism and seed protein contnt.Promptly measure the nitrogen content of analyte earlier, multiply by 6.25 coefficients again and promptly draw protein contnt) contain 15%-25% respectively, the protein of 25%-37.5% in the soybean stem, leaf.If contained proteins extraction in its cauline leaf is come out, will develop the soy proteinaceous new resources of a kind of production.

It is that raw material extracts soy proteinaceous method with the bright cauline leaf of soybean that summary of the invention the object of the present invention is to provide a kind of, and present method technology is simple, and extraction yield is high, develops a kind of new way for producing soy-protein.

Technical scheme of the present invention is following:

This process for extracting is made up of following steps:

1, gather: when soybean plant strain gets into initial bloom stage, the above bright cauline leaf in its face of land of gathering;

2, clean: plant is cleaned up with impacting current and spray water;

3, pull an oar: the cauline leaf after will cleaning with hollander breaks into pulpous state liquid in small, broken bits;

4, squeezing: pulpous state liquid is added in the squeezing machine pressure 24-25MPa, the juice in the fiber in small, broken bits of squeezing out;

5, filter: expressed juice through pressure filter 100 order nets, is leached impurity;

6, heat and filter or fermentation: heat or use microwave hyperthermia with steam, the temperature of juice is risen to 70 ℃-80 ℃ rapidly, and kept temperature 3-5 minute, protein is met the heat back and is produced and solidifies, and precipitates; Then temperature is reduced to 38--42 ℃ rapidly, leach sedimentary protein; Or employing fermentation method: the lactobacillus suspension that promptly adds its weight 1% in the juice after filter; Anaerobic fermentation 24-48 hour at normal temperatures; With sodium hydroxide liquid is transferred to PH6.8-7; In with 20 minutes, the lactic acid that utilizes lactobacillus spp to produce makes protein coagulating, deposition in the juice, then sedimentary protein is leached;

7, take off raw meat: the crude protein that leaches is packed in the container, inject crude protein volume 2-3 water doubly, then liquid is warming up to 95 ℃-98 ℃ rapidly; And kept temperature 46 minutes, through heating, trypsinase and lipoxidase are lost activity; Both can improve proteinic absorption rate; Can remove beany flavor again, simultaneously, also play sterilization functions;

8, separate: the crude protein that will take off after the raw meat is put into whizzer, and rotating speed is 5000-10000 rev/min, separates into the wet cake of water cut 60%;

9, drying: the wet cake of isolated crude protein is sent in the spray-dryer, and the spout place temperature of kiln is 120 ℃, and bottom temp is 80 ℃, and the micropowder of atomizing is dried when falling;

10, sterilization: the crude protein powder that will reduce to normal temperature is used ultraviolet disinfection;

11, packing: store in compound bag that the crude protein powder after will sterilizing is packed into moistureproof.

The invention has the advantages that: 1, present method is from bright cauline leaf, to extract protein; Cauline leaf is just gathered at initial bloom stage; Bright cauline leaf of 2-3 season can be planted in the place of the north former 1 year one season of kind soybean; Can obtain the higher biological yield of unit surface, and then obtain the very high plant crude protein output of unit surface, for the exploitation vegetable-protein provides a kind of feasible approach.2, use this process for extracting, technology is simple, and equipment used also is a conventional equipment, and extraction yield is higher.3, the crude protein powder finished product that extracts with bright cauline leaf contains several mineral materials and trace element, and the sampling observation result of part mineral substance, trace element shows that its content is not less than the soybean of equivalent.Both making it to become to provide plant protein, can replenish the food of other nutrition again.Face assay as follows.

Through the soy-protein product that aforesaid method obtained; Detect applied code: GB/T 5009.5-2003, GB/T 5009.6-2003, GB/T 5009.4-2003, GB/T 5009.10-2003, GB/T 5009.13-2003, GB/T5009.90-2003, GB/T 5009.90-2003, GB/T 5009.14-2003, GB/T 5009.92-2003, GB/T 5009.90-2003, GB/T 18246-2000 through agricultural product quality and safety supervision and inspection center of the Ministry of Agriculture (Huhehaote).

Detected result: its gross protein value reaches 80.1%, crude fat 4.39%, robust fibre 0.22%, coarse ash 4.66%, calcium 1.85%, copper 21.8mg/kg, iron 464mg/kg, manganese 22mg/kg, zinc 62mg/kg, magnesium 575mg/kg.

Embodiment

This process for extracting is made up of following steps:

1, gather: when soybean plant strain gets into initial bloom stage, its bright cauline leaf of gathering;

2, clean: plant is cleaned up with impacting current and spray water;

4, squeezing: pulpous state liquid is added in the squeezing machine pressure 24.5MPa, the juice in the fiber in small, broken bits of squeezing out;

5, filter: expressed juice is leached impurity through pressure filter 100 order nets;

6, adopt steam to heat, steam is directly imported in the juice, when heating, rise to 70 ℃-80 ℃ to the temperature of juice rapidly, and kept temperature 3-5 minute, protein chance heat back produces solidifies, and deposition; Then, its temperature is reduced to 40 ℃ rapidly, leach sedimentary protein with cold water;

7, take off raw meat: the crude protein that leaches is packed in the container of heating, inject crude protein volume 2-3 water doubly, then liquid is warming up to 95 ℃-98 ℃ rapidly; And kept temperature 4-6 minute, through heating, trypsinase and lipoxidase are lost activity; Both can improve proteinic absorption rate; Can remove beany flavor again, simultaneously, also play sterilization functions;

9, drying: the wet cake of isolated crude protein is sent in the spray-dryer, and the spout place temperature of kiln is 120 ℃, and bottom temp is 80 ℃, and the trifling of atomizing is dried when falling;

10, sterilization: will reduce to the normal temperature external appearance characteristic and use ultraviolet disinfection for green crude protein powder;

Claims

1. one kind is that raw material extracts soy proteinaceous method with the bright cauline leaf of soybean, and it is characterized in that: it is made up of following steps:

A, gather: when soybean plant strain gets into initial bloom stage, the above bright cauline leaf in its face of land of gathering;

B, cleaning: bright cauline leaf is cleaned up with impacting current and spray water;

C, making beating: the cauline leaf after will cleaning with hollander breaks into pulpous state liquid in small, broken bits;

D, squeezing: pulpous state liquid is added in the squeezing machine pressure 24-25MPa, the juice in the fiber in small, broken bits of squeezing out;

E, filtration: expressed juice through pressure filter 100 order nets, is leached impurity;

F, heat and filter or fermentation: heat or use microwave hyperthermia with steam, the temperature of juice is risen to 70 ℃-80 ℃ rapidly, and kept temperature 3-5 minute, protein is met the heat back and is produced and solidifies, and precipitates; Then temperature is reduced to 38--42 ℃ rapidly, leach sedimentary protein; Or employing fermentation method: the lactobacillus suspension that promptly adds its weight 1% in the juice after filter; Anaerobic fermentation 24-48 hour at normal temperatures; With sodium hydroxide liquid is transferred to PH6.8-7; In with 20 minutes, the lactic acid that utilizes milk-acid bacteria to produce makes protein coagulating, deposition in the juice, then sedimentary protein is leached;

G, take off raw meat: the crude protein that leaches is packed in the container, inject crude protein volume 2-3 water doubly, then liquid is warming up to 95 ℃-98 ℃ rapidly; And kept temperature 4-6 minute, through heating, trypsinase and lipoxidase are lost activity; Both can improve proteinic absorption rate; Can remove beany flavor again, simultaneously, also play sterilization functions;

H, separation: the crude protein that will take off after the raw meat is put into whizzer, and rotating speed is 5000-10000 rev/min, separates into the wet cake of water cut 60%;

I, drying: the wet cake of isolated crude protein is sent in the spray-dryer, and the spout place temperature of kiln is 120 ℃, and bottom temp is 80 ℃, and the micropowder of atomizing is dried when falling;

J, sterilization: the crude protein powder that will reduce to normal temperature is used ultraviolet disinfection; With

K, packing: store in compound bag that the crude protein powder after will sterilizing is packed into moistureproof.