CN101743979A - Bactericidal composite with active ester - Google Patents

Abstract

The invention relates to a bactericidal composite with active ester. The active ingredients of the bactericidal composite are active ester (A) and one substance (B) of zoxamide, penthiopyrad and mandipropamid in a weight ratio of 100:1-1:100, wherein the content of the active ingredients of the composite is 5-80wt%. The composite can be used to prepare suspending agent, wettable powder, water dispersing granule and other dosage forms. Farmlands, orchards and other places are suitable for using the bactericidal composite of the invention, and the bactericidal composite is used to prevent and cure various plant diseases of grains, vegetables, fruit trees, flowers and other crops, in particular to prevent and cure downy mildew and blight.

Description

A kind of bactericidal composition that contains Acibenzolar

Technical field

The present invention relates to a kind of bactericidal composition, especially contain the bactericidal composition of Acibenzolar and zoxamide or pyrrole metsulfovax or mandipropamid amine, composition has protection, prevention and therapeutic action, belongs to the disinfectant use in agriculture field.

Background technology

Downy mildew is a kind of most important disease on gourd, fruit and vegetable is produced, and especially takes place general with cucumber, sponge gourd, balsam pear, muskmelon in melon and is injured heavier.Downy mildew is the very strong disease of a kind of popularity, in the vegetable-growing area of not planting disease-resistant variety, and light underproduction 30-40% then when disease is popular, serious reaches more than the 70-80%, so that ruin kind.

Downy mildew all can take place from seedling to each stage of results, is injured heavier to become strain.The blade of mainly causing harm is developed to upper leaf by base portion.Their early stage forms light yellow subcircular to the polygonal scab on the blade face, blade back produced the mould layer of white shape when there was dampness in the air, can spread to the blade face sometimes.The later stage scab is in flakes withered, is yellowish-brown, the withered and yellow death of whole siphonal lobes when serious.Morbidities easily such as field planting is overstocked, it is too early, excessive to water after the field planting, soil moisture big, impeded drainage.Spring is early summer or the easiest generation of continuous rainy weather in autumn not.

Eqpidemic disease mainly is meant by the fungus-caused class disease of Phytophthora (Phytophthora), mainly cause plant flowers, really, the quick necrosis of leaf portion tissue and rotting.High humidity is to influence the principal element that disease takes place and propagates.Wherein melon eqpidemic disease and potato late blight are important diseases.

In the control of downy mildew and eqpidemic disease, mainly rely on metalaxyl, dimethomorph and some protectiveness medicaments.These medicaments are because long-term use has produced pesticide resistance in various degree, and control efficiency reduces.A large amount of uses of medicament have simultaneously increased residue of pesticide, and the harm food security has increased the weight of environmental pollution.

Acibenzolar is a diazosulfide carboxylic acid esters plant disease-resistant activator.Acibenzolar English name: acibenzolar chemical name: benzo (1,2,3) thiadiazoles-7-carbothioic acid-S-methyl esters test code number CGA245704, be called for short BTH molecular formula: C ₈H ₆N ₂OS ₂

Acibenzolar obtains resistance by the defense response that activates crop self, thereby resists diseases such as powdery mildew, downy mildew, rust.Suitable crop has paddy rice, wheat, vegetables, banana, tobacco etc.

Pesticide active ingredient applied in any combination with Acibenzolar and other structure type, there is the composition of synergistic effect not only can obviously improve actual preventive effect, reduce dosage and cost, also help to avoid the generation and the suspension of pesticide resistance generation speed of germ resistance, thereby solve problems such as resistance in the present agricultural chemicals list agent application process and cost.

Summary of the invention

The object of the present invention is to provide that a kind of new resistance risk is little, cost is low, good drug efficacy, long bactericidal composition of lasting period.

The inventor is by a large amount of indoor biometrics and field control effectiveness tests, after in unexpected discovery Acibenzolar and acid amide fungicides zoxamide, pyrrole metsulfovax, the mandipropamid amine any one is mixed in certain proportion, diseases such as downy mildew, eqpidemic disease there is significant synergistic effect.

Described zoxamide: english common name zoxamide, chemical name (RS)-3,5-two chloro-N-(3-chloro-1-ethyl-1-methyl-2-oxopropyl) be to methyl benzamide, molecular formula C ₁₄H ₁₆Cl ₃NO ₂

Zoxamide (Zoxamide) is a kind of acid amide fungicides of protectiveness efficiently, and the lasting period is long, has good resistance of rainwater washing against performance, is mainly used in and prevents and treats Oomycete disease such as eqpidemic disease, downy mildew etc., and is also effective to gray mold, powdery mildew.Its mechanism of action uniqueness is by suppressing the sclerotium division in conjunction with tubulin β-subunit and destruction microtubule cytoskeleton.

Described pyrrole metsulfovax: English name Penthiopyrad, chemical name (RS)-N-[2-(1, the 3-dimethylbutyl)-3-thiophene phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazole-4-carboxamide, molecular formula: C ₁₆H ₂₀F ₃N ₃OS.

Pyrrole metsulfovax controlling object rust, stalk break, gray mold, downy mildew, apple scab and powdery mildew etc.The pyrrole metsulfovax is at 100～200g (a.i.)/hm ²Under the dosage, cauline leaf is handled can prevent and treat apple scab, powdery mildew etc. effectively.Under 100mg/L concentration, grape grey mould there is fine activity, under the 25mg/L concentration cucumber downy mildew is had good control efficiency.

Described mandipropamid amine: English name mandipropamid, chemical name 2-(4-chloro-phenyl)-N-[2-(3-methoxyl group-4-(2-third alkynyloxy group)-phenyl-ethyl group]-2-(2-third alkynyloxy group)-acetamide, molecular formula C ₂₃H ₂₂CLNO ₄

Mandipropamid amine is acid amide fungicides.Its mechanism of action is for suppressing the biosynthesis of phosphatide, and leaf portion and fruit disease that exhausted big number is caused by oomycetes all have good preventive effect.The spore that is in the sprouting stage is had higher activity, and can suppress mycelia and grow up and sporulation.Can be absorbed rapidly by blade, and be rested in the leaf table wax coat, blade is shielded.

The inventor is on the basis of above-mentioned discovery, through composition is carried out symphyogenetic quantitative analysis, formed technical scheme of the present invention, that is: a kind of bactericidal composition, the mass ratio of Acibenzolar in the composition (A) and zoxamide or pyrrole metsulfovax or mandipropamid amine (B) is 100: 1～1: 100, and A is 40: 1～1: 40 with B quality optimization ratio.Active component gross mass percentage composition is 5～80% in the composition.

The present composition can be prepared into known method and be fit to any one formulation that agricultural is used, and formulation has suspending agent, wetting powder, water dispersible granules etc. preferably.These preparations can be prepared by method in common, for example, active substance is mixed with liquid flux and/or solid carrier, add surfactant such as emulsifier, dispersant, stabilizing agent, wetting agent simultaneously, can also add adhesive, defoamer, oxidant, dyestuff etc.

For suspending agent, one or more in spendable dispersant such as polycarboxylate, lignosulfonates such as lignin sulfonic acid sodium salt, the alkylnaphthalene sulfonate; Among emulsifier such as Nongru-700 # (common name alkyl phenol formaldehyde resin polyoxyethylene ether), nekal formaldehyde condensation products, agricultural newborn 2201#, this dish-60# (common name sorbitol anhydride monostearate), agricultural newborn 1601# (common name triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), the TERSPERSE4894 (2500) one or more; In wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products phosphate, the withered powder of tea, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, spaonin powder, silkworm excrement, the soapberry powder one or more; In thickener such as xanthans, polyvinyl alcohol, bentonite, CMC, methylcellulose, the Magnesiumaluminumsilicate one or more; In preservative such as formaldehyde, benzoic acid, the Sodium Benzoate one or more; Defoamer such as organic silicon defoamer; In antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as the sodium chloride one or more.

For water dispersible granules, one or more in spendable dispersant such as polycarboxylate, lignosulfonates, NNO4, the alkylnaphthalene sulfonate; In wetting agent such as polyoxyethylene nonylphenol ether, polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In disintegrant such as ammonium sulfate, urea, sucrose, the glucose one or more; In binding agent such as diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose one or more; In filler such as diatomite, calcium bicarbonate, kaolin, white carbon, talcum powder, the potter's clay one or more.

For wetting powder, in spendable dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, the potter's clay one or more.

The present composition can be the finished product preparation form to be provided, and promptly each material mixes in the composition; The composition of composition also can provide with single agent formulation, directly mixes in bucket (jar) before using.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.

Composition of the present invention can be used for preventing and treating the important disease that is caused by sac fungi, oomycetes etc., is particularly suitable for preventing and treating downy mildew, eqpidemic disease.It is farmland, orchard etc. that composition of the present invention is used the place.

Composition of the present invention also can with other compound with weeding, desinsection or bactericidal property particularly protective fungicide mix use, also can mix use with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner.

The present invention comprises that also a kind of scene kills the method for fungi, before or after crop and/or fruit are susceptible, uses composition of the present invention to the place of crop and/or fruit and growth or storage.Can use by common method, as cast, injection, spraying, dusting, distribution.Amount of application of the present invention is with weather condition or crop state variation.The duration of protective effect is usually relevant with the content of individualized compound in the composition, and is relevant with extraneous factor, weather for example, but by using suitable formulation can slow down climatic influences.

Composition of the present invention possesses following advantage: the one, and the composition synergistic effect is obvious, and drug effect significantly improves; The 2nd, the composition drug effect improves, and the field consumption descends, and has reduced production and use cost, reduces residue of pesticide and environmental pollution; The 3rd, composition is made up of the active ingredient of different mechanism of action, and action site increases, and helps overcoming and delays the drug-fast generation of germ.The 4th, enlarged fungicidal spectrum, can prevent and treat downy mildew disease such as powdery mildew in addition.

Embodiment

Different pesticide active ingredients shows three kinds of type of action: summation action, synergistic effect and antagonism after mixing usually.But be specially which kind of effect, unpredictable, have only by a large amount of tests and just can know.Compound synergic is well filled a prescription, owing to obviously improved actual control efficiency, reduced the usage amount of agricultural chemicals, thereby delayed the drug-fast generation speed of germ widely, and be the important means of scientific prevention and cure disease.

The inventor has carried out a large amount of indoor bioassay test and field control effectiveness test, find that Acibenzolar and zoxamide or pyrrole metsulfovax or the combination of mandipropamid amine afterwards have remarkable role in synergy to Pseudoperonospora cubensis etc., and be not only the simple addition (seeing biologicall test example 1 for details) of two kinds of medicaments.

Biologicall test embodiment 1: the composite Toxicity Determination of Acibenzolar and zoxamide or pyrrole metsulfovax or mandipropamid amine to bacterium of downy mildew of cucumber

Subjects: bacterium of downy mildew of cucumber (Pseudoperonospora cubensis)

Pot-culture method is adopted in this test.Choose two leaf period cucumber seedlings of growing way unanimity, each processing selects for use 5 basins for examination melon seedling, numbers standby.Under 50PSI pressure, spray every basin 5mL with the Potter spray tower.Each medicament is provided with 5 concentration gradients, is blank with what spray the equivalent clear water.Spray inoculation bacterium of downy mildew of cucumber sporangia suspension behind the chemicals treatment 24h, the layoutprocedure of sporangia suspension is as follows: get the cucumber leaves that has Pseudoperonospora cubensis that picks up from the field, dip in the sporangium that the distilled water of getting about 10 ℃ washes the back side with writing brush, being made into concentration is 3 * 10 ⁵The sporangia suspension of individual/mL.Place climatic cabinate (relative moisture 100%, temperature 15-20 ℃) to cultivate cucumber seedling after the inoculation, keep 15-24 ℃ of temperature, relative moisture about 90% to preserve moisture and bring out behind the 24h, 10d " Invest, Then Investigate " record incidence calculates disease index and control efficiency.

The cucumber downy mildew grade scale:

0 grade: blade does not have scab;

1 grade: lesion area accounts for below 5% of whole blade area;

3 grades: lesion area accounts for the 6-10% of whole blade area;

5 grades: lesion area accounts for the 11-25% of whole blade area;

7 grades: lesion area accounts for the 26-50% of whole blade area;

9 grades: lesion area accounts for more than 50% of whole blade area.

Calculate concentration EC in the inhibition with method of least squares ₅₀, calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.

Actual measurement toxicity index (ATI)=(standard medicament EC ₅₀/ reagent agent EC ₅₀) * 100

The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (the ATI)/theoretical toxicity index (TTI) of mixture] * 100.

CTC≤80 are antagonism, and 80＜CTC＜120 are summation action, and CTC 〉=120 are synergistic effect.

As can be known from Table 1, Acibenzolar and pyrrole metsulfovax are composite, are between 100: 1～1: 100 the time in proportioning, and co-toxicity coefficient all more than 120, has synergistic effect to bacterium of downy mildew of cucumber, and in the time of especially between 40: 1～1: 40, co-toxicity coefficient is more than 190.

The composite results from pot experiment test of table 1 Acibenzolar and pyrrole metsulfovax to bacterium of downy mildew of cucumber

Handle	??EC 50??(μg/ml)	??ATI	??TTI	Co-toxicity coefficient (CTC)
					Acibenzolar (A)	??5.17	??100	??/	??/
Pyrrole metsulfovax (B)	??9.38	??55.12	??/	??/
					??A∶B(100∶1)	??3.69	??140.11	??99.56	??140.73
??A∶B(70∶1)	??4.07	??127.03	??99.37	??127.84
					??A∶B(40∶1)	??2.65	??195.09	??98.91	??197.25
??A∶B(15∶1)	??2.76	??187.32	??97.19	??192.73
					??A∶B(1∶1)	??3.28	??157.62	??77.56	??203.23

Handle	??EC 50??(μg/ml)	??ATI	??TTI	Co-toxicity coefficient (CTC)
					??A∶B(1∶15)	??4.27	??121.08	??57.92	??209.03
??A∶B(1∶40)	??4.69	??110.23	??56.21	??196.11
					??A∶B(1∶70)	??6.37	??81.16	??55.75	??145.58
??A∶B(1∶100)	??6.78	??76.25	??55.56	??137.24

As can be known from Table 2, Acibenzolar and mandipropamid amine are composite, are between 100: 1～1: 100 the time in proportioning, and co-toxicity coefficient all more than 120, has synergistic effect to bacterium of downy mildew of cucumber, and in the time of especially between 40: 1～1: 40, co-toxicity coefficient is more than 210.

The composite results from pot experiment test of table 2 Acibenzolar and mandipropamid amine to bacterium of downy mildew of cucumber

Handle	??EC 50??(μg/ml??)	??ATI	??TTI	Co-toxicity coefficient (CTC)
					Acibenzolar (A)	??5.21	??100	??/	??/
Mandipropamid amine (B)	??7.15	??72.87	??/	??/
					??A∶B(100∶1)	??3.57	??145.94	??99.73	??146.33
??A∶B(70∶1)	??3.95	??131.90	??99.62	??132.40
					??A∶B(40∶1)	??2.43	??214.40	??99.34	??215.83
??A∶B(15∶1)	??2.51	??207.57	??98.30	??211.15
					??A∶B(1∶1)	??2.78	??187.41	??86.43	??216.83
??A∶B(1∶15)	??3.16	??164.87	??74.56	??221.12
					??A∶B(1∶40)	??3.28	??158.84	??73.53	??216.02
??A∶B(1∶70)	??5.09	??102.36	??73.25	??139.74
					??A∶B(1∶100)	??5.37	??97.02	??73.14	??132.66

As can be known from Table 3, Acibenzolar and zoxamide are composite, are between 100: 1～1: 100 the time in proportioning, and co-toxicity coefficient all more than 120, has synergistic effect to bacterium of downy mildew of cucumber, and in the time of especially between 40: 1～1: 40, co-toxicity coefficient is more than 190.

The composite results from pot experiment test of table 3 Acibenzolar and zoxamide to bacterium of downy mildew of cucumber

Handle	??EC 50??(μg/ml)	??ATI	??TTI	Co-toxicity coefficient (CTC)
					Acibenzolar (A)	??6.38	??100	??/	??/
Zoxamide (B)	??8.75	??72.91	??/	??/
					??A∶B(100∶1)	??4.63	??137.80	??99.73	??138.17
??A∶B(75∶1)	??5.09	??125.34	??99.64	??125.79
					??A∶B(40∶1)	??3.17	??201.26	??99.34	??202.60
??A∶B(15∶1)	??3.35	??190.45	??98.31	??193.73
					??A∶B(1∶1)	??3.57	??178.71	??86.46	??206.71
??A∶B(1∶15)	??4.36	??146.33	??74.61	??196.13
					??A∶B(1∶40)	??4.49	??142.09	??73.57	??193.13
??A∶B(1∶75)	??5.86	??108.87	??73.27	??148.59
					??A∶B(1∶100)	??6.12	??104.25	??73.18	??142.45

Below further specify the present invention with specific embodiment, but the present invention is limited to these examples absolutely not.The following stated only is the present invention embodiment preferably, only in order to explain the present invention, can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that all any modifications of being made within the spirit and principles in the present invention, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Percentage composition is mass percent in all preparation proportionings.

The suspending agent example

Press formula rate, the ratio of each component such as active component, dispersant, wetting agent, thickener, antifreeze and water in prescription mixed, after grinding or high speed shear, make suspending agent.

Embodiment 1:20.2% Acibenzolar pyrrole metsulfovax suspending agent

Acibenzolar 20%, pyrrole metsulfovax 0.2%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 10%, lauryl sodium sulfate (wetting agent) 3%, xanthans (thickener) 2%, bentonite (thickener) 1%, glycerine (antifreeze) 5%, water complements to 100%.

Embodiment 2:10.1% Acibenzolar pyrrole metsulfovax suspending agent

Acibenzolar 0.1%, pyrrole metsulfovax 10%, polycarboxylate (dispersant) 10%, the withered powder of tea (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, ethylene glycol (antifreeze) 5%, water complements to 100%.

Embodiment 3:30.3% Acibenzolar mandipropamid amine suspending agent

Acibenzolar 30%, mandipropamid amine 0.3%, lignin sulfonic acid sodium salt (dispersant) 10%, lauryl sodium sulfate (wetting agent) 3%, xanthans (thickener) 2%, polyvinyl alcohol (thickener) 1%, propane diols, (antifreeze) 5%, water complements to 100%.

Embodiment 4:10.1% Acibenzolar mandipropamid amine suspending agent

Acibenzolar 0.1%, mandipropamid amine 10%, alkylnaphthalene sulfonate (dispersant) 10%, the withered powder of tea (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, urea (antifreeze) 5%, water complements to 100%.

Embodiment 5:20.2% Acibenzolar zoxamide suspending agent

Acibenzolar 20%, zoxamide 0.2%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 10%, alkylsulfonate (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, xanthans (thickener) 1%, glycerine (antifreeze) 5%, water complements to 100%.

Embodiment 6:30.3% Acibenzolar zoxamide suspending agent

Acibenzolar 0.3%, zoxamide 30%, polycarboxylate (dispersant) 10%, alkyl sulfate (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, xanthans (thickener) 1%, ethylene glycol (antifreeze) 5%, water complements to 100%.

Embodiment 7:5% Acibenzolar pyrrole metsulfovax suspending agent

Acibenzolar 3%, pyrrole metsulfovax 2%, polycarboxylate (dispersant) 10%, lauryl sodium sulfate (wetting agent) 3%, xanthans (thickener) 2%, bentonite (thickener) 1%, urea (antifreeze) 5%, water complements to 100%.

Embodiment 8:5% Acibenzolar mandipropamid amine suspending agent

Acibenzolar 1%, mandipropamid amine 4%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 10%, lauryl sodium sulfate (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, xanthans (thickener) 1%, sodium chloride (antifreeze) 5%, water complements to 100%.

Embodiment 9:5% Acibenzolar zoxamide suspending agent

Acibenzolar 2.5%, zoxamide 2.5%, polycarboxylate (dispersant) 10%, spaonin powder (wetting agent) 3%, xanthans (thickener) 2%, bentonite (thickener) 1%, urea (antifreeze) 5%, water complements to 100%.

Embodiment 10:41% Acibenzolar pyrrole metsulfovax suspending agent

Acibenzolar 40%, pyrrole metsulfovax 1%, polycarboxylate (dispersant) 10%,, silkworm excrement (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, propane diols (antifreeze) 5%, water complements to 100%.

Embodiment 11:20.5% Acibenzolar mandipropamid amine suspending agent

Acibenzolar 0.5%, mandipropamid amine 20%, polycarboxylate (dispersant) 10%, soapberry powder (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, glycerine (antifreeze) 5%, water complements to 100%.

Embodiment 12:41% Acibenzolar zoxamide suspending agent

Acibenzolar 40%, zoxamide 1%, polycarboxylate (dispersant) 10%,, silkworm excrement (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, xanthans (thickener) 1%, urea (antifreeze) 5%, water complements to 100%.

The wetting powder example

Pesticide activity component, auxiliary agent, filler are mixed the mixed again wetting powder that gets behind comminution by gas stream in proportion.Capital equipment: mixer, airslide disintegrating mill.

Embodiment 13:80% Acibenzolar pyrrole metsulfovax wetting powder

Acibenzolar 60%, pyrrole metsulfovax 20%, lauryl sodium sulfate (wetting agent) 2%, sodium lignin sulfonate (dispersant) 3%, white carbon black 10% (filler), kaolin complements to 100%.

Embodiment 14:80% Acibenzolar mandipropamid amine wetting powder

Acibenzolar 30%, mandipropamid amine 50%, lauryl sodium sulfate (wetting agent) 2%, sodium lignin sulfonate (dispersant) 3%, diatomite 10% (filler), potter's clay complements to 100%.

Embodiment 15:80% Acibenzolar zoxamide wetting powder

Acibenzolar 40%, zoxamide 40%, lauryl sodium sulfate (wetting agent) 2%, sodium lignin sulfonate (dispersant) 3%, talcum powder 10% (filler), attapulgite complements to 100%.

Embodiment 16:20.5% Acibenzolar pyrrole metsulfovax wetting powder

Acibenzolar 0.5%, pyrrole metsulfovax 20%, alkyl sulfate (wetting agent) 3%, polycarboxylate (dispersant) 3%, white carbon black (filler) 11%, bentonite complements to 100%.

Embodiment 17:41% Acibenzolar mandipropamid amine wetting powder

Acibenzolar 40%, mandipropamid amine 1%, alkyl sulfate (wetting agent) 3%, polycarboxylate (dispersant) 3%, kaolin (filler) 11%, attapulgite complements to 100%.

Embodiment 18:20.5% Acibenzolar zoxamide wetting powder

Acibenzolar 0.5%, zoxamide 20%, alkyl sulfate (wetting agent) 3%, polycarboxylate (dispersant) 3%, talcum powder (filler) 11%, bentonite complements to 100%.

Embodiment 19:70% Acibenzolar pyrrole metsulfovax wetting powder

Acibenzolar 40%, pyrrole metsulfovax 30%, alkylsulfonate (wetting agent) 3%, sodium lignin sulfonate (dispersant) 3%, white carbon black (filler) 11%, potter's clay complements to 100%.

The water dispersible granules example

Press formula rate, former medicine and powder carrier, moistening spreader-sticker and binding agent etc. are mixed pulverizing, after adding water again and mediating, add in the comminutor that the certain specification screen cloth is housed and carry out granulation.And then drying, screening (pressing the screen cloth scope) promptly get granular product.

Embodiment 20:30% Acibenzolar pyrrole metsulfovax water dispersible granules

Acibenzolar 10%, pyrrole metsulfovax 20%, polycarboxylate (dispersant) 5%, sodium lignin sulfonate (dispersant) 7%, lauryl sodium sulfate (wetting agent) 2%, ammonium sulfate (disintegrant) 5%, precipitated calcium carbonate complements to 100%.

Embodiment 21:40% Acibenzolar mandipropamid amine water dispersible granules

Acibenzolar 5%, mandipropamid amine 35%, Negel (dispersant) 5%, sodium lignin sulfonate (dispersant) 7%, lauryl sodium sulfate (wetting agent) 2%, urea (disintegrant) 5%, precipitated calcium carbonate complements to 100%.

Embodiment 22:35% Acibenzolar zoxamide water dispersible granules

Acibenzolar 20%, zoxamide 15%, Negel (dispersant) 5%, sodium lignin sulfonate (dispersant) 7%, lauryl sodium sulfate (wetting agent) 2%, glucose (disintegrant) 5%, precipitated calcium carbonate complements to 100%.

Embodiment 23:20% Acibenzolar mandipropamid amine water dispersible granules

Acibenzolar 18%, mandipropamid amine 2%, polycarboxylate (dispersant) 5%, sodium lignin sulfonate (dispersant) 7%, lauryl sodium sulfate (wetting agent) 2%, ammonium sulfate (disintegrant) 5%, precipitated calcium carbonate complements to 100%.

Field application examples 1: the composite field control effectiveness test of Acibenzolar and pyrrole metsulfovax to cucumber downy mildew

Choose the cucumber downy mildew heavier plot of morbidity throughout the year.Test medicine and consumption see Table 4, and 20 square metres of each sub-district areas are repeated in 4 sub-districts of every processing.Carry out the dispenser first time at the disease initial stage of origination, the complete stool even spraying till being sprayed onto blade and dripping, is treated to blank with clear water.Execute medicine for the second time after 7 days.The incidence of 7 days investigation cucumber downy mildews behind the medicine before first time medicine and for the second time.5 samplings are adopted in every sub-district, every some investigation 2 strains, and all blades are investigated in every strain, and the percentage that accounts for whole leaf area with lesion area comes the classification record.Calculate and respectively handle disease index and control efficiency.

Grade scale:

0 grade: no scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for the 6%-15% of whole leaf area;

5 grades: lesion area accounts for the 16%-25% of whole leaf area;

7 grades: lesion area accounts for the 26%-50% of whole leaf area;

9 grades: lesion area accounts for more than 50% of whole leaf area.

The drug effect computational methods:

As can be seen from Table 4: Acibenzolar, each the single agent of pyrrole metsulfovax to the preventive effect of cucumber downy mildew be respectively 74.47% and 77.68%,, and embodiment of the invention bactericidal composition all is significantly increased on preventive effect, minimum preventive effect is 85.69%, has been up to 90.30%.The result of field trial shows that fully the composite back of Acibenzolar and pyrrole metsulfovax has remarkable role in synergy to cucumber downy mildew, and under the situation that the mu effective ingredient consumption reduces, control efficiency significantly improves.Therefore, the present composition have reduce cost, the beneficial effect of delaying drug resistance, minimizing spraying times and residue of pesticide.

The composite field test results of table 4 Acibenzolar and pyrrole metsulfovax to cucumber downy mildew

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					50% Acibenzolar wetting powder	??50	??4.5	??12.3	??74.47
20% pyrrole metsulfovax suspending agent	??150	??4.1	??9.8	??77.68

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					Example of formulations 1 (20.2% Acibenzolar pyrrole metsulfovax suspending agent)	??45	??3.9	??5.7	??86.35
Example of formulations 2 (10.1% Acibenzolar pyrrole metsulfovax suspending agent)	??65	??4.7	??7.2	??85.69
					Example of formulations 7 (5% Acibenzolar pyrrole metsulfovax suspending agent)	??42	??3.5	??4.7	??87.46
Example of formulations 10 (41% Acibenzolar pyrrole	??40	??5.2	??5.4	??90.30
					The metsulfovax suspending agent)
Example of formulations 13 (80% Acibenzolar pyrrole metsulfovax wetting powder)	??45	??4.3	??5.5	??88.06
					Example of formulations 16 (20.5% Acibenzolar pyrrole metsulfovax wetting powder)	??48	??3.7	??5.4	??86.37
Example of formulations 20 (30% Acibenzolar pyrrole metsulfovax water dispersible granules)	??48	??5.3	??6.9	??87.84
					??CK	??-	??4.8	??51.4	??-

Field application examples 2: the composite field control effectiveness test of Acibenzolar and mandipropamid amine to downy mildew of garpe

Choose the downy mildew of garpe heavier plot of morbidity throughout the year.Test medicine and consumption see Table 5, and 20 square metres of each sub-district areas are repeated in 4 sub-districts of every processing.Carry out the dispenser first time at the disease initial stage of origination, the complete stool even spraying till being sprayed onto blade and dripping, is treated to blank with clear water.Execute medicine for the second time after 7 days.The incidence of 7 days investigation downys mildew of garpe behind the medicine before first time medicine and for the second time.5 samplings are adopted in every sub-district, every some investigation 3 strains, and all blades are investigated in every strain, and the percentage that accounts for whole leaf area with lesion area comes the classification record.Calculate and respectively handle disease index and control efficiency.

Grade scale:

0 grade: no scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for the 6%-15% of whole leaf area;

5 grades: lesion area accounts for the 16%-25% of whole leaf area;

7 grades: lesion area accounts for the 26%-50% of whole leaf area;

9 grades: lesion area accounts for more than 50% of whole leaf area.

The drug effect computational methods:

As can be seen from Table 5: Acibenzolar, each the single agent of mandipropamid amine to the preventive effect of downy mildew of garpe be respectively 75.10% and 79.49%,, and embodiment of the invention bactericidal composition all is significantly increased on preventive effect, minimum preventive effect is 86.30%, has been up to 91.35%.The result of field trial shows that fully the composite back of Acibenzolar and mandipropamid amine has remarkable role in synergy to downy mildew of garpe, and under the situation that the mu effective ingredient consumption reduces, control efficiency significantly improves.Therefore, the present composition have reduce cost, the beneficial effect of delaying drug resistance, minimizing spraying times and residue of pesticide.

The composite field test results of table 5 Acibenzolar and mandipropamid amine to downy mildew of garpe

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					50% Acibenzolar wetting powder	??50	??5.1	??13.8	??75.10
250 grams per liter mandipropamid amine suspending agents	??150	??4.8	??10.7	??79.49
					Example of formulations 3 (30.3% Acibenzolar mandipropamid amine suspending agent)	??45	??4.5	??6.7	??86.30
Example of formulations 4 (10.1% Acibenzolar mandipropamid amine suspending agent)	??60	??4.7	??6.5	??87.27

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					Example of formulations 8 (5% Acibenzolar mandipropamid amine suspending agent)	??45	??5.4	??6.6	??88.75
Example of formulations 11 (20.5% Acibenzolar mandipropamid amine suspending agent)	??45	??4.4	??6.4	??86.62
					Example of formulations 14 (80% Acibenzolar mandipropamid amine wetting powder)	??48	??3.9	??5.3	??87.49
Example of formulations 17 (41% Acibenzolar mandipropamid amine wetting powder)	??40	??5.0	??4.7	??91.35
					Example of formulations 21 (40% Acibenzolar mandipropamid amine water dispersible granules)	??45	??3.6	??4.9	??87.48
??CK	??-	??5.3	??57.6	??-

Field application examples 3: the composite field control effectiveness test of Acibenzolar and zoxamide to tobacco downy mildew

Choose the tobacco downy mildew heavier plot of morbidity throughout the year.Test medicine and consumption see Table 6, and 20 square metres of each sub-district areas are repeated in 4 sub-districts of every processing.Carry out the dispenser first time at the disease initial stage of origination, the complete stool even spraying till being sprayed onto blade and dripping, is treated to blank with clear water.Execute medicine for the second time after 7 days.The incidence of 7 days investigation tobacco downy mildews behind the medicine before first time medicine and for the second time.5 samplings are adopted in every sub-district, every some investigation 2 strains, and all blades are investigated in every strain, and the percentage that accounts for whole leaf area with lesion area comes the classification record.Calculate and respectively handle disease index and control efficiency.

Grade scale:

0 grade: no scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for the 6%-15% of whole leaf area;

5 grades: lesion area accounts for the 16%-25% of whole leaf area;

7 grades: lesion area accounts for the 26%-50% of whole leaf area;

9 grades: lesion area accounts for more than 50% of whole leaf area.

The drug effect computational methods:

As can be seen from Table 6: Acibenzolar, each single agent of zoxamide to the preventive effect of tobacco downy mildew be respectively 74.36% and 75.14%,, and embodiment of the invention bactericidal composition all is significantly increased on preventive effect, minimum preventive effect is 85.22%, has been up to 89.11%.The result of field trial shows that fully the composite back of Acibenzolar and zoxamide has remarkable role in synergy to tobacco downy mildew, and under the situation that the mu effective ingredient consumption reduces, control efficiency significantly improves.Therefore, the present composition have reduce cost, the beneficial effect of delaying drug resistance, minimizing spraying times and residue of pesticide.

The composite field test results of table 6 Acibenzolar and zoxamide to tobacco downy mildew

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					50% Acibenzolar wetting powder	??50	??4.1	??12.8	??74.36
24% zoxamide suspending agent	??250	??3.6	??10.9	??75.14
					Example of formulations 5 (20.2% Acibenzolar zoxamide suspending agent)	??45	??4.5	??7.6	??86.13
Example of formulations 6 (30.3% Acibenzolar zoxamide suspending agent)	??48	??4.9	??7.4	??87.60
					Example of formulations 9 (5% Acibenzolar zoxamide suspending agent)	??45	??3.5	??6.3	??85.22
Example of formulations 12 (41% Acibenzolar zoxamide suspending agent)	??40	??4.6	??6.1	??89.11

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					Example of formulations 15 (80% Acibenzolar zoxamide wetting powder)	??42	??5.1	??7.5	??87.93
Example of formulations 18 (20.5% Acibenzolar zoxamide wetting powder)	??48	??3.6	??6.2	??85.86
					Example of formulations 22 (35% Acibenzolar zoxamide water dispersible granules)	??45	??4.7	??7.8	??86.37
??CK	??-	??3.9	??47.5	??-

Claims (6)

1. bactericidal composition is characterized in that: active ingredient be Acibenzolar (A) with zoxamide, pyrrole metsulfovax, mandipropamid amine in a kind of (B).

2. bactericidal composition according to claim 1 is characterized in that: preferred 100: 1～1: 100 of the mass ratio of effective constituents A and B.

3. bactericidal composition according to claim 2 is characterized in that: preferred 40: 1～1: 40 of the mass ratio of effective constituents A and B.

4. according to each described bactericidal composition of claim 1-3, it is characterized in that: active component quality percentage composition is 5～80% in the composition.

5. bactericidal composition according to claim 1 is characterized in that: said composition can be mixed with suspending agent, wetting powder, water dispersible granules.

6. the described bactericidal composition of claim 1 is used to prevent and treat the disease on cereal, vegetables, fruit tree, the flowers.