CN101732105A - Fast propagation expansion and large-scale health culture method of fine breed cow - Google Patents
Fast propagation expansion and large-scale health culture method of fine breed cow Download PDFInfo
- Publication number
- CN101732105A CN101732105A CN200810194629A CN200810194629A CN101732105A CN 101732105 A CN101732105 A CN 101732105A CN 200810194629 A CN200810194629 A CN 200810194629A CN 200810194629 A CN200810194629 A CN 200810194629A CN 101732105 A CN101732105 A CN 101732105A
- Authority
- CN
- China
- Prior art keywords
- cow
- embryo
- rate
- technology
- fine breed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a fast propagation expansion and large-scale health culture method of fine breed cow, which is characterized by using superovulation technology to carry out in vivo embryo production and using living body egg taking technology and vitro fertilization technology for industrialized production of in vitro embryos. In addition, the living body egg taking technology and vitro fertilization technology are combined, and sex-controlling semen is used to produce female embryos, so that breeding potential of high quality and high yield cows can be fully made use of, and a large number of fine breed cow female embryos featuring clear-cut pedigree, low price and high conception rate can be obtained; as far as fast propagation expansion of fine breed cows is concerned, a fine breed cow core group is established by selective breeding, and a large-scale health culture technological system for fine breed cows is established, therefore, per unit yield of each cow and milk quality are improved.
Description
Technical field
The present invention relates to a kind of animal varieties improving technology, say exactly to the invention discloses quick expanding propagation of a kind of fine breed cow and large-scale health culture method.
Background technology
The embryo engineering technology is that (embryo transfer is the basis ET), the general name of a series of biotechnology by manual operation embryo, directed change animal character with embryo transfer.It mainly comprises superovulation, embryo transfer, embryo's batch production production, sex identification, freezing preservation, embryo cloning and gene transfer etc.And embryo's batch production production comprises ovum pick-up (ovum pick-up, OPU), oocyte in vitro maturation is cultivated (in vitro maturation, IVM), external fertilization (in vitro fertilization, IVF) and in-vitro oosperm grow to cultivate (invitro culture, link such as IVC).Since the seventies in 20th century, embryo's biotechnology development both at home and abroad in some developed countries, has formed commercialization, industrialization as the application of state embryo biotechnologies such as the U.S., Canada, Japan rapidly.In the also existing 20 years of researches history of China's animal embryo biotechnology, the major technique level near or reach world-class levels, embryo and produced in vitro embryo come into the market in the domestic existing milch cow body now.According to world's embryo transfer association (IETA) incomplete statistics in 2000, the whole world has 80,000 pieces of embryos to be used for outlet every year approximately, and 1999 are only about 40,000 pieces of Canada outlet cattle embryo.
At present, domestic very big to high yield cow embryo demand, only depend on the interior embryo of body not satisfy the demands, reason has two: (1) import cow embryo costs an arm and a leg, and quarantine measures is loaded down with trivial details, wastes time and energy; (2) homemade cow embryo is by conventional superovulation method, and the cycle is long, and adult cow every year only can be towards embryo 4～5 times, and production capacity is lower, can not satisfy demand domestic and that export.And former some problems that pre-exist of external fertilization embryo, as: external fertilization embryo genetic background (pedigree) is unclear, the transplanting conception rate is low etc., continuous research raising along with ovum pick-up, zooblast In vitro culture and embryo gender authenticate technology, to progressively be overcome, not only can obtain the embryo that a large amount of pedigrees are clear, price is lower by ovum pick-up-external fertilization approach, and, the cost of embryo transfer will be reduced greatly along with the raising and the sex controll The Application of Technology of external fertilization embryo transfer conception rate.Therefore, fast, the research and development prospect of efficient cow embryo batch production production is boundless.
Summary of the invention
The invention discloses quick expanding propagation of a kind of fine breed cow and large-scale health culture method; be primarily aimed at the problem of to meet the need of market by embryo in the body; and solve the germ cell embryo transfer and germ cell is carried out sex identification by ovum pick-up-external fertilization approach; improve conception rate and the sex controll technology of transplanting, to reduce the embryo transfer cost.Mainly comprise: utilize the superovulation technology to carry out Embryo Production in the body, utilize ovum pick-up technology and the batch production of in vitro fertilization technology to produce external embryo; It is characterized in that: the ovum pick-up technology is combined with the external fertilization technology, and application control seminal fluid is produced female embryo, the breeding potential of good quality and high output milch cow is given full play to, can be obtained the fine breed cow female embryo that a large amount of pedigrees are clear, cheap, conception rate is higher.The superovulation technology is mainly grasped according to following technical parameter:
(1) embryo's production and implantation technique parameter in the milch cow body
Towards embryo 3 months at interval
On average obtain 7.5 pieces of available embryo numbers every time
Embryo gender control rate (female) 95%
Bright embryonic implantation pregnancy rate 65%
Bright embryonic implantation survival rate 55%
Freeze embryonic implantation pregnancy rate 55%
Freeze embryonic implantation survival rate 45%
(2) the external embryo's of milch cow production and implantation technique parameter
Ovum pick-up 1-2 time weekly at interval
On average adopt ovum and count 6-8 piece for every time
Oocyte in Vitro fertilization blastocyst rate reaches 35-40%;
Embryo gender control rate (female) 95%
Bright embryonic implantation pregnancy rate 40%
Bright embryonic implantation survival rate 35%
Freeze embryonic implantation pregnancy rate 35%
Freeze embryonic implantation survival rate 30%
(3) milch cow production target
Cow begins to utilize 15 months ages
Cow economic utilization time limit 6-8
The cow breeding rate 90% that is used for ovum pick-up
Average 285 days trimester of pregnancy
Several 1 year 1 tire of breeding tire
Annual milk yield ≮ 8000 kilogram/head;
Fresh milk butterfat percnetage ≮ 3.2%;
Cow in milk lactation period is 305 days;
Become milk cattle year to eliminate turnover rate 10%-15%;
The calf 2.5-3 monthly age weans, 1-6 monthly age survival rate 95%, growing cattle, young cattle survival rate 98%.
Because multinomial advanced technology assembling match is adopted in the research, its achievement is with high content of technology, and is practical, can obtain good economy, society and environmental benefit.
Description of drawings
Referring to accompanying drawing, be technical matters flow chart of the present invention.
Embodiment
Embodiment one, Embryo Production in the body, select the breeding donor by from the fine breed cow core group, having ready conditions, use embryo's biotechnology (superovulation technology, artificial fertilization technology, non-operation are towards ovum technology, embryo's cutting techniques, embryo gender control technology) and obtain the fine breed cow female embryo, pass through embryo transfer technology, be implanted into through the recipient cattle uterus of selecting, estrus synchronization is handled, obtain a large amount of breeding offsprings by receptor cow gestation.Perhaps will pass through embryo's quick freezing technology through non-operation towards the breeding embryo (bright embryo) that embryo obtains in a large number, freezing preservation, according to receptor cow quantity situation, at any time carry out one and go on foot the transplanting of thawing, overcome time, regional condition restriction, can carry out the commercial operations that cow embryo is transplanted to greatest extent.
Embodiment two, external Embryo Production, use the ovum pick-up instrument fine breed cow is implemented ovum pick-up (OPU), the oocyte of collection is cultivated (IVM), external fertilization (IVF), germ cell In vitro culture (IVC) productivity control embryo through maturation in vitro, and female embryo is transplanted (ET).Feature of the present invention combines the ovum pick-up technology exactly with the external fertilization technology, and application control seminal fluid is produced female embryo, the breeding potential of good quality and high output milch cow is given full play to, can be obtained the fine breed cow female embryo that a large amount of pedigrees are clear, cheap, conception rate is higher.
Claims (2)
1. quick expanding propagation of fine breed cow and large-scale health culture method mainly comprise: the ovum pick-up technology is combined with the external fertilization technology, and application control seminal fluid is produced female embryo; It is characterized in that: solve the germ cell embryo transfer and germ cell is carried out sex identification by ovum pick-up-external fertilization approach, improve conception rate and the sex controll technology of transplanting, to reduce the embryo transfer cost.
2. quick expanding propagation of a kind of fine breed cow according to claim 1 and large-scale health culture method, its feature are that also described ovum pick-up-external fertilization mainly realizes according to following technical parameter:
(1) embryo's production and implantation technique parameter in the milch cow body
Towards embryo 3 months at interval
On average obtain 7.5 pieces of available embryo numbers every time
Embryo gender control rate (female) 95%
Bright embryonic implantation pregnancy rate 65%
Bright embryonic implantation survival rate 55%
Freeze embryonic implantation pregnancy rate 55%
Freeze embryonic implantation survival rate 45%
(2) the external embryo's of milch cow production and implantation technique parameter
Ovum pick-up 1-2 time weekly at interval
On average adopt ovum and count 6-8 piece for every time
Oocyte in Vitro fertilization blastocyst rate reaches 35-40%;
Embryo gender control rate (female) 95%
Bright embryonic implantation pregnancy rate 40%
Bright embryonic implantation survival rate 35%
Freeze embryonic implantation pregnancy rate 35%
Freeze embryonic implantation survival rate 30%
(3) milch cow production target
Cow begins to utilize 15 months ages
Cow economic utilization time limit 6-8
The cow breeding rate 90% that is used for ovum pick-up
Average 285 days trimester of pregnancy
Several 1 year 1 tire of breeding tire
Annual milk yield ≮ 8000 kilogram/head;
Fresh milk butterfat percnetage ≮ 3.2%;
Cow in milk lactation period is 305 days;
Become milk cattle year to eliminate turnover rate 10%-15%;
The calf 2.5-3 monthly age weans, 1-6 monthly age survival rate 95%, growing cattle, young cattle survival rate 98%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810194629A CN101732105A (en) | 2008-11-14 | 2008-11-14 | Fast propagation expansion and large-scale health culture method of fine breed cow |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810194629A CN101732105A (en) | 2008-11-14 | 2008-11-14 | Fast propagation expansion and large-scale health culture method of fine breed cow |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101732105A true CN101732105A (en) | 2010-06-16 |
Family
ID=42456486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810194629A Pending CN101732105A (en) | 2008-11-14 | 2008-11-14 | Fast propagation expansion and large-scale health culture method of fine breed cow |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101732105A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039404A (en) * | 2012-12-06 | 2013-04-17 | 云南省草地动物科学研究院 | Breeding method for heat-resisting beef cattle |
| CN103598146A (en) * | 2013-10-15 | 2014-02-26 | 天津市奶牛发展中心 | Method for cultivating excellent breeding oxen through genomic selection and sex control embryo technology |
-
2008
- 2008-11-14 CN CN200810194629A patent/CN101732105A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039404A (en) * | 2012-12-06 | 2013-04-17 | 云南省草地动物科学研究院 | Breeding method for heat-resisting beef cattle |
| CN103039404B (en) * | 2012-12-06 | 2015-09-23 | 云南省草地动物科学研究院 | The selection of heat-resisting beef cattle |
| CN103598146A (en) * | 2013-10-15 | 2014-02-26 | 天津市奶牛发展中心 | Method for cultivating excellent breeding oxen through genomic selection and sex control embryo technology |
| CN103598146B (en) * | 2013-10-15 | 2016-09-28 | 天津市奶牛发展中心 | A kind of method utilizing full-length genome to select to cultivate good species bull with sex control embryo technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Perera | Reproduction in domestic buffalo | |
| CN102972319B (en) | Method for establishment of hybrid strain between bluntsnout bream and topmouth culter and breeding method of topmouth bream | |
| CN102140435A (en) | Method for improving in-vitro production efficiency of buffalo embryos | |
| CN100334205C (en) | Method for producing sex controllable in vitro embryo of buffalo | |
| CN103875574A (en) | Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes | |
| CN107549059B (en) | Hybrid breeding method for female sturgeon and male paddlefish | |
| CN103184189A (en) | Cultivation method of cross-bred wagy | |
| CN102106279A (en) | Hybrid seedlings-cultivating method for improving growth traits of siniperca scherzeri | |
| CN101627737B (en) | Method for establishing blue crab family and selecting improved breed | |
| CN101732105A (en) | Fast propagation expansion and large-scale health culture method of fine breed cow | |
| CN105557586A (en) | Hybrid breeding method for female Siberian sturgeon and male Amur sturgeon | |
| CN106305522B (en) | A kind of artificial raise seedling method of more precious fishes | |
| CN103215220A (en) | Method for producing dzo gender controllable in vitro embryos | |
| CN101843530A (en) | Method for fertilizing oocytes in vitro of young animals twice | |
| CN113767896B (en) | Bovine in vitro fertilization embryo vitrification freezing method | |
| CN101911919A (en) | Hybrid breeding method for Chinese stichopus japonicus and red stichopus japonicus | |
| CN110684720B (en) | Method for obtaining male pronucleus by taking oocyte among heterogeneous animals as medium | |
| CN1226378A (en) | Technology for crossbreeding sheep in 'tubes' | |
| Patel et al. | Implication of embryo transfer technology in livestock productivity | |
| CN110283780B (en) | Method for collecting superovulation ovum of living cattle and preparation used by method | |
| CN101962627A (en) | Method for producing in-vitro calf embryo | |
| CN105900920A (en) | Cultivating method for improving farrowing capacity of sows | |
| CN1226377A (en) | Process for industrializing technology of 'tube cattle' | |
| CN102321670A (en) | Method for cloning somatic cells of Bama miniature pig | |
| CN116218764A (en) | Cow embryo production and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100616 |