CN101712966A - Method for producing monascus-pigment in liquid submerged fermentation - Google Patents

Method for producing monascus-pigment in liquid submerged fermentation Download PDF

Info

Publication number
CN101712966A
CN101712966A CN200910033624A CN200910033624A CN101712966A CN 101712966 A CN101712966 A CN 101712966A CN 200910033624 A CN200910033624 A CN 200910033624A CN 200910033624 A CN200910033624 A CN 200910033624A CN 101712966 A CN101712966 A CN 101712966A
Authority
CN
China
Prior art keywords
pigment
corn
monascus
submerged fermentation
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200910033624A
Other languages
Chinese (zh)
Inventor
彭常安
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN200910033624A priority Critical patent/CN101712966A/en
Publication of CN101712966A publication Critical patent/CN101712966A/en
Pending legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a method for producing monascus-pigment in liquid submerged fermentation, which takes corns as the main raw material, adopts monascus purpureus to produce the optimum culture medium composition of the monascus purpureus by liquid submerged fermentation and relative technological conditions selection. The shaking flask result shows that in terms of the optimum culture medium formulation which contains 8-10% of inoculum size and is cultured for 96h under the temperature of 32 DEG C, the haematochrome color value of the fermentation liquor is stabilized at 120mu/ml basically, and the maximum value is 150-160mu/ml, wet thallus yield accounts for 12% of the fermentation liquor, and the color value of the wet thallus is 800-1000mu/g. By using the method, the product has the advantages of high color value, short production period, high utilization rate of raw materials and less environment pollution.

Description

A kind of method of producing monascus-pigment in liquid submerged fermentation
Technical field:
The invention belongs to the foodstuff additive industrial circle, relate to a kind of production method of monascorubin.
Technical background:
Monascorubin is the major ingredient in the red colouring agent for food, also used as a Chinese medicine, and it is had many uses, and can be used as the natural colorant of foodstuff additive, also can be used as the health care medical material.At present, the method of producing monascorubin mainly contains two kinds, a kind of is the solid state fermentation extraction method, Monascus anka Nakazawa et sato is seeded in the rice after the boiling, forms through solid-state cultivation and fermentation, and grind after the drying, form with organic solvent extraction such as alcohol pigment drying wherein again, it is less investment that solid state fermentation is produced the monascorubin biggest advantage, and maximum shortcoming is that labour intensity is big, and the cycle is long.Another kind method is the liquid state fermentation extraction method, and the rice fragmentation is equipped with other auxiliary material and adds water, and inoculation red colouring agent for food, also used as a Chinese medicine bacterial classification is after liquid state fermentation, and concentrate drying forms after filtration, and the phase is with short production cycle, and weak point is for producing look valency instability, and the cost utilization ratio is not high.
Summary of the invention:
The objective of the invention is to overcome the deficiency that prior art exists, the employing corn is a carbon source, and a kind of product look valency height is provided, and is with short production cycle, raw material availability height, the monascorubin preparation method that environmental pollution is little.
The present invention is by following skill
The art scheme realizes:
(1) wort by 5-6Be ' adds 2% agar, PH nature, sterilization preparation slant medium under the condition of 0.1Mpa/30min;
(2) adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium;
(3) by in the 1000ml triangular flask, adding the 25g millet, 30ml water, sterilization preparation millet substratum under the condition of 0.1Mpa/60min;
(4) the inclined-plane seed is cultivated on the millet substratum, and culture condition is 32 ℃, 5-7 days, obtains spore suspension;
(5) press Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O 0.05%, PH nature, sterilising conditions is 0.1Mpa/30min, preparation shake-flask seed substratum;
(6) configuration fermention medium: corn 10%, NH4NO3 0.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O 0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
(7) liquid amount is that 250ml triangular flask loading amount is 50ml, and inoculum size is 8%-10%, and training method adopts rotary vibrator shaking culture, rotating speed 150rpm, and culture temperature is 32 ℃, incubation time 3-4 days
(8) high luminance relay valency monascorubin powder is produced: 80% monascorubin is present in the mycelium, and 20% is present in the fermented liquid, and most of pigment is present in the mycelium, directly adopts 70% ethanol lixiviate 1 hour, filters, concentrates, lyophilize makes.
Embodiment:
Adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium; Wort at 5-6Be ' adds 2% agar, PH nature, the culture medium culturing of sterilizing under the condition of 0.1Mpa/30min; The inclined-plane seed was obtained the millet spore in 5-7 days in the cultivation under 32 ℃ of environment of cultivation on the millet substratum, pressing Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O 0.05%, the PH nature, sterilising conditions is 0.1Mpa/30min, cultivate 7-10 days (32 ℃) on the shake-flask seed substratum of preparation, again liquid seeds is cultivated 30-36hrs (32 ℃) on fermention medium, liquid amount is that 250ml triangular flask loading amount is 50ml, inoculum size is 9%, training method adopts rotary vibrator shaking culture, rotating speed 150rpm, culture temperature is 32 ℃, incubation time 3-4 days, and then through filtering, concentrate, lyophilize makes product.
It mainly is with reference to the influence of different carbon sources to monascorubin as carbon source that corn is adopted in these research and development
Carbon source kind Look valency (OD) Tone Raw material availability (%)
Corn ??112.5 Cherry-red ??80.9
Rice ??97.8 Bright red ??64.7
Sweet potato ??140.. Garnet ??75.0
The fermention medium that adopts is to obtain best of breed by orthogonal test, obtains red colouring agent for food, also used as a Chinese medicine look valency height, stable and culture cycle weak point, has improved raw material availability greatly.The best of breed prescription of present embodiment is: corn 10%, NH4NO3 0.30%, GA 0.40%, KH2PO43H2O 0.10%, MgSO47H2O 0.05%, corn steep liquor 1.0%, PH5.0-5.5.

Claims (4)

1. the method for a producing monascus-pigment in liquid submerged fermentation is characterized in that comprising following rapid step by step:
(1) wort by 5-6Be ' adds 2% agar, PH nature, sterilization preparation slant medium under the condition of 0.1Mpa/30min;
(2) adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium;
(3) by in the 1000ml triangular flask, adding the 25g millet, 30ml water, sterilization preparation millet substratum under the condition of 0.1Mpa/60min;
(4) the inclined-plane seed is cultivated on the millet substratum, and culture condition is 32 ℃, 5-7 days, cultivates into spore suspension;
(5) press Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O0.05%, PH nature, sterilising conditions is 0.1Mpa/30min, preparation shake-flask seed substratum;
(6) spore is carried out enlarged culturing on seed culture medium;
(7) configuration fermention medium: corn 10%, NH4NO30.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
(8) liquid amount is that 250ml triangular flask loading amount is 50ml, and inoculum size is 8%-10%, and training method adopts rotary vibrator shaking culture, rotating speed 150rpm, and culture temperature is 32 ℃, incubation time 3-4 days
(9) high luminance relay valency monascorubin powder is produced: 80% monascorubin is present in the mycelium, and 20% is present in the fermented liquid, and most of pigment is present in the mycelium, directly adopts 70% ethanol lixiviate 1 hour, filters, concentrates, lyophilize makes.
2. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1 is characterized in the corn that is chosen as of fermention medium carbon source.
3. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1, prescription is adopted in the preparation that is characterized in fermention medium: corn 10%, NH4NO30.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
4. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1, be characterized in fermenting liquid amount and inoculum size are 250ml triangular flask liquid amount 50ml and 8%-10%.
CN200910033624A 2009-06-25 2009-06-25 Method for producing monascus-pigment in liquid submerged fermentation Pending CN101712966A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN200910033624A CN101712966A (en) 2009-06-25 2009-06-25 Method for producing monascus-pigment in liquid submerged fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN200910033624A CN101712966A (en) 2009-06-25 2009-06-25 Method for producing monascus-pigment in liquid submerged fermentation

Publications (1)

Publication Number Publication Date
CN101712966A true CN101712966A (en) 2010-05-26

Family

ID=42416965

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200910033624A Pending CN101712966A (en) 2009-06-25 2009-06-25 Method for producing monascus-pigment in liquid submerged fermentation

Country Status (1)

Country Link
CN (1) CN101712966A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102653721A (en) * 2011-08-15 2012-09-05 东莞市天益生物发酵技术有限公司 Method for preparing monascus mycelia with function of reducing blood fat by liquid state fermentation of grains
CN104987972A (en) * 2015-03-31 2015-10-21 福建师范大学 Preparation method of liquid fermentum rubrum for brewing
CN107723320A (en) * 2017-09-08 2018-02-23 广东科隆生物科技有限公司 A kind of method for directly preparing crystal red yeast rice citraurin

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102653721A (en) * 2011-08-15 2012-09-05 东莞市天益生物发酵技术有限公司 Method for preparing monascus mycelia with function of reducing blood fat by liquid state fermentation of grains
CN104987972A (en) * 2015-03-31 2015-10-21 福建师范大学 Preparation method of liquid fermentum rubrum for brewing
CN107723320A (en) * 2017-09-08 2018-02-23 广东科隆生物科技有限公司 A kind of method for directly preparing crystal red yeast rice citraurin
CN107723320B (en) * 2017-09-08 2020-09-18 广东科隆生物科技有限公司 Method for directly preparing crystal monascus orange pigment

Similar Documents

Publication Publication Date Title
CN102703358B (en) Preparation method of bacillus high-temperature yeast
CN103734482B (en) The production method of a kind of feed addictive " mortierella Diding culture "
CN102154407B (en) Corayceps militaris polysaccharide two-stage fermentation synthesis process
CN104694590B (en) The fermentation preparation of gamma-polyglutamic acid-and the bacterial strain of product gamma-polyglutamic acid-
CN104893983B (en) Liquid state fermentation low citrinin, the preparation method of High color values monascorubin and product
CN103858670A (en) Method for cultivating pleurotus geesteranus
CN103483040B (en) Chinese caterpillar fungus pilot scale liquid submerged fermentation substratum and fermentation method for producing thereof
CN103289937B (en) Method for producing bacillus laterosporus live bacteria by high density solid fermentation
CN103194410B (en) Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same
CN101830757B (en) Vetiver substrate for culturing edible mushrooms
CN103232946B (en) High-tolerance ester-producing yeast strain and application thereof
CN109486881A (en) A kind of fermentation medium and zymotechnique of kasugarnycin
CN104206169A (en) Method for preparing nutrient cereal by cordyceps militaris culture medium
CN102173893A (en) Method for preparing edible fungi substrate by utilizing waste bamboo shells
CN105349436A (en) Preparation method of wild tricholoma matsutake domesticated mother strains
CN103087893A (en) Preparation method of composite coarse cereals monascus
CN103992977A (en) Method for culturing bacillus subtilis through high-density fermentation
CN101712966A (en) Method for producing monascus-pigment in liquid submerged fermentation
CN103005437B (en) Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation
CN107177515A (en) A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation
CN106755179A (en) A kind of culture medium for being suitable to bacteria cellulose fermentation
CN106431658A (en) Oyster mushroom culture medium and oyster mushroom cultivation method
CN115152527B (en) Method for cultivating high-quality cordyceps militaris fruiting bodies in short period
CN110669678A (en) Griseofulvin strain solid culture medium
CN102533604B (en) Brevibacterium flavum and application thereof as well as method for preparing lysine through fermentation

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20100526