CN101712966A - Method for producing monascus-pigment in liquid submerged fermentation - Google Patents
Method for producing monascus-pigment in liquid submerged fermentation Download PDFInfo
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- CN101712966A CN101712966A CN200910033624A CN200910033624A CN101712966A CN 101712966 A CN101712966 A CN 101712966A CN 200910033624 A CN200910033624 A CN 200910033624A CN 200910033624 A CN200910033624 A CN 200910033624A CN 101712966 A CN101712966 A CN 101712966A
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Abstract
The invention discloses a method for producing monascus-pigment in liquid submerged fermentation, which takes corns as the main raw material, adopts monascus purpureus to produce the optimum culture medium composition of the monascus purpureus by liquid submerged fermentation and relative technological conditions selection. The shaking flask result shows that in terms of the optimum culture medium formulation which contains 8-10% of inoculum size and is cultured for 96h under the temperature of 32 DEG C, the haematochrome color value of the fermentation liquor is stabilized at 120mu/ml basically, and the maximum value is 150-160mu/ml, wet thallus yield accounts for 12% of the fermentation liquor, and the color value of the wet thallus is 800-1000mu/g. By using the method, the product has the advantages of high color value, short production period, high utilization rate of raw materials and less environment pollution.
Description
Technical field:
The invention belongs to the foodstuff additive industrial circle, relate to a kind of production method of monascorubin.
Technical background:
Monascorubin is the major ingredient in the red colouring agent for food, also used as a Chinese medicine, and it is had many uses, and can be used as the natural colorant of foodstuff additive, also can be used as the health care medical material.At present, the method of producing monascorubin mainly contains two kinds, a kind of is the solid state fermentation extraction method, Monascus anka Nakazawa et sato is seeded in the rice after the boiling, forms through solid-state cultivation and fermentation, and grind after the drying, form with organic solvent extraction such as alcohol pigment drying wherein again, it is less investment that solid state fermentation is produced the monascorubin biggest advantage, and maximum shortcoming is that labour intensity is big, and the cycle is long.Another kind method is the liquid state fermentation extraction method, and the rice fragmentation is equipped with other auxiliary material and adds water, and inoculation red colouring agent for food, also used as a Chinese medicine bacterial classification is after liquid state fermentation, and concentrate drying forms after filtration, and the phase is with short production cycle, and weak point is for producing look valency instability, and the cost utilization ratio is not high.
Summary of the invention:
The objective of the invention is to overcome the deficiency that prior art exists, the employing corn is a carbon source, and a kind of product look valency height is provided, and is with short production cycle, raw material availability height, the monascorubin preparation method that environmental pollution is little.
The present invention is by following skill
The art scheme realizes:
(1) wort by 5-6Be ' adds 2% agar, PH nature, sterilization preparation slant medium under the condition of 0.1Mpa/30min;
(2) adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium;
(3) by in the 1000ml triangular flask, adding the 25g millet, 30ml water, sterilization preparation millet substratum under the condition of 0.1Mpa/60min;
(4) the inclined-plane seed is cultivated on the millet substratum, and culture condition is 32 ℃, 5-7 days, obtains spore suspension;
(5) press Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O 0.05%, PH nature, sterilising conditions is 0.1Mpa/30min, preparation shake-flask seed substratum;
(6) configuration fermention medium: corn 10%, NH4NO3 0.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O 0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
(7) liquid amount is that 250ml triangular flask loading amount is 50ml, and inoculum size is 8%-10%, and training method adopts rotary vibrator shaking culture, rotating speed 150rpm, and culture temperature is 32 ℃, incubation time 3-4 days
(8) high luminance relay valency monascorubin powder is produced: 80% monascorubin is present in the mycelium, and 20% is present in the fermented liquid, and most of pigment is present in the mycelium, directly adopts 70% ethanol lixiviate 1 hour, filters, concentrates, lyophilize makes.
Embodiment:
Adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium; Wort at 5-6Be ' adds 2% agar, PH nature, the culture medium culturing of sterilizing under the condition of 0.1Mpa/30min; The inclined-plane seed was obtained the millet spore in 5-7 days in the cultivation under 32 ℃ of environment of cultivation on the millet substratum, pressing Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O 0.05%, the PH nature, sterilising conditions is 0.1Mpa/30min, cultivate 7-10 days (32 ℃) on the shake-flask seed substratum of preparation, again liquid seeds is cultivated 30-36hrs (32 ℃) on fermention medium, liquid amount is that 250ml triangular flask loading amount is 50ml, inoculum size is 9%, training method adopts rotary vibrator shaking culture, rotating speed 150rpm, culture temperature is 32 ℃, incubation time 3-4 days, and then through filtering, concentrate, lyophilize makes product.
It mainly is with reference to the influence of different carbon sources to monascorubin as carbon source that corn is adopted in these research and development
| Carbon source kind | Look valency (OD) | Tone | Raw material availability (%) |
| Corn | ??112.5 | Cherry-red | ??80.9 |
| Rice | ??97.8 | Bright red | ??64.7 |
| Sweet potato | ??140.. | Garnet | ??75.0 |
The fermention medium that adopts is to obtain best of breed by orthogonal test, obtains red colouring agent for food, also used as a Chinese medicine look valency height, stable and culture cycle weak point, has improved raw material availability greatly.The best of breed prescription of present embodiment is: corn 10%, NH4NO3 0.30%, GA 0.40%, KH2PO43H2O 0.10%, MgSO47H2O 0.05%, corn steep liquor 1.0%, PH5.0-5.5.
Claims (4)
1. the method for a producing monascus-pigment in liquid submerged fermentation is characterized in that comprising following rapid step by step:
(1) wort by 5-6Be ' adds 2% agar, PH nature, sterilization preparation slant medium under the condition of 0.1Mpa/30min;
(2) adopt the method for line that monascus ruber is inoculated into preparation inclined-plane seed on the slant medium;
(3) by in the 1000ml triangular flask, adding the 25g millet, 30ml water, sterilization preparation millet substratum under the condition of 0.1Mpa/60min;
(4) the inclined-plane seed is cultivated on the millet substratum, and culture condition is 32 ℃, 5-7 days, cultivates into spore suspension;
(5) press Zulkovsky starch 3%, SODIUMNITRATE 0.30%, yeast extract paste 0.50%, KH2PO4 0.10%, MgSO47H2O0.05%, PH nature, sterilising conditions is 0.1Mpa/30min, preparation shake-flask seed substratum;
(6) spore is carried out enlarged culturing on seed culture medium;
(7) configuration fermention medium: corn 10%, NH4NO30.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
(8) liquid amount is that 250ml triangular flask loading amount is 50ml, and inoculum size is 8%-10%, and training method adopts rotary vibrator shaking culture, rotating speed 150rpm, and culture temperature is 32 ℃, incubation time 3-4 days
(9) high luminance relay valency monascorubin powder is produced: 80% monascorubin is present in the mycelium, and 20% is present in the fermented liquid, and most of pigment is present in the mycelium, directly adopts 70% ethanol lixiviate 1 hour, filters, concentrates, lyophilize makes.
2. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1 is characterized in the corn that is chosen as of fermention medium carbon source.
3. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1, prescription is adopted in the preparation that is characterized in fermention medium: corn 10%, NH4NO30.30%, monosodium glutamate (GA crystal) 0.40%, KH2PO43H2O 0.10%, MgSO47H2O0.05%, corn steep liquor 1.0%, PH5.0-5.5, corn fiber crops powder is sieved through 80 orders, add water and size mixing the back at 0.12Mpa eyeball gelatinization 1hr, add saccharifying enzyme saccharification 10min after being cooled to 60 ℃, sterilize when adding other raw material and regulating PH to 5.0-5.5, sterilising conditions is 0.1Mpa/30min.
4. the method for a kind of producing monascus-pigment in liquid submerged fermentation according to claim 1, be characterized in fermenting liquid amount and inoculum size are 250ml triangular flask liquid amount 50ml and 8%-10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910033624A CN101712966A (en) | 2009-06-25 | 2009-06-25 | Method for producing monascus-pigment in liquid submerged fermentation |
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| CN200910033624A CN101712966A (en) | 2009-06-25 | 2009-06-25 | Method for producing monascus-pigment in liquid submerged fermentation |
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| CN200910033624A Pending CN101712966A (en) | 2009-06-25 | 2009-06-25 | Method for producing monascus-pigment in liquid submerged fermentation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653721A (en) * | 2011-08-15 | 2012-09-05 | 东莞市天益生物发酵技术有限公司 | Method for preparing monascus mycelia with function of reducing blood fat by liquid state fermentation of grains |
| CN104987972A (en) * | 2015-03-31 | 2015-10-21 | 福建师范大学 | Preparation method of liquid fermentum rubrum for brewing |
| CN107723320A (en) * | 2017-09-08 | 2018-02-23 | 广东科隆生物科技有限公司 | A kind of method for directly preparing crystal red yeast rice citraurin |
-
2009
- 2009-06-25 CN CN200910033624A patent/CN101712966A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653721A (en) * | 2011-08-15 | 2012-09-05 | 东莞市天益生物发酵技术有限公司 | Method for preparing monascus mycelia with function of reducing blood fat by liquid state fermentation of grains |
| CN104987972A (en) * | 2015-03-31 | 2015-10-21 | 福建师范大学 | Preparation method of liquid fermentum rubrum for brewing |
| CN107723320A (en) * | 2017-09-08 | 2018-02-23 | 广东科隆生物科技有限公司 | A kind of method for directly preparing crystal red yeast rice citraurin |
| CN107723320B (en) * | 2017-09-08 | 2020-09-18 | 广东科隆生物科技有限公司 | Method for directly preparing crystal monascus orange pigment |
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Open date: 20100526 |