CN101709265B - Cell culture reactor - Google Patents
Cell culture reactor Download PDFInfo
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- CN101709265B CN101709265B CN 200910210478 CN200910210478A CN101709265B CN 101709265 B CN101709265 B CN 101709265B CN 200910210478 CN200910210478 CN 200910210478 CN 200910210478 A CN200910210478 A CN 200910210478A CN 101709265 B CN101709265 B CN 101709265B
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- cell culture
- gas
- tank body
- gas distributor
- whisking appliance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- Bioinformatics & Cheminformatics (AREA)
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- Chemical & Material Sciences (AREA)
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- Biomedical Technology (AREA)
- Sustainable Development (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a cell culture reactor, comprising a tank body and a deep layer ventilation module arranged in the tank body; the deep layer ventilation module comprises an air circulating pipe and an air diffuser, the air diffuser is arranged at one end of the air circulating pipe, close to the bottom part of the tank body, and the other end of the air circulating pipe is connected withan air supplying device, and a stirrer is arranged below the air diffuser; the cell culture reactor can reduce the air bubble generation probability after the air flows in a reactor, reduce the effect of air bubble on the cells and improve the cell density in the reactor.
Description
Technical field
The present invention relates to a kind of cell culture reactor, specifically a kind of gas feeding inside reactor can reduce later the bubble generation and gas can be diffused into the cell culture reactor in the tank body equably.
Background technology
The mode of in cell culture reactor, ventilating in the prior art, generally has two kinds: a kind of for directly ventilating in reactor drum through pipeline; Another kind is in reactor drum, to be provided with closed pipeline, on closed pipeline, bores some aperture ventilations then, and this pipeline also is called as sparger.
More than these two kinds of modes of in reactor drum, ventilating all in reactor drum, produce bubble easily, and bubble breaks in cell cultures liquid and can cause cell injury even death, thereby influences the cell density in the reactor drum.
Summary of the invention
The object of the present invention is to provide a kind of cell culture reactor, reduce gas and feed the probability that reactor drum produces bubble later on, reduce the break influence of pair cell of bubble, improve the cell density in the reactor drum.
The present invention realizes through following technical scheme: cell culture reactor; Comprise tank body; Also comprise and be arranged on said jar of intravital deep ventilation module; Said deep ventilation module comprises the gentle body decollator of gas stream siphunculus, and said gas distributor is installed on the end that said gas stream siphunculus closes on said tank base, and the other end of said gas stream siphunculus is connected with air feeder; The below of said gas distributor is provided with whisking appliance.
Above-mentioned cell culture reactor, the center of said gas distributor is on the medullary ray of said whisking appliance.
Cell culture reactor, the axis of said tank body and the medullary ray of the said whisking appliance angle C between the two is 10～20 degree.
Above-mentioned cell culture reactor, the axis of said tank body and the medullary ray of the said whisking appliance angle C between the two is 15 degree.
Above-mentioned cell culture reactor, the length B of said gas distributor are 1/2nd to 2/3rds of said agitator paddle diameter D.
Above-mentioned cell culture reactor, the distance A of said gas distributor and said agitator paddle are 100 millimeters～200 millimeters.
Above-mentioned cell culture reactor, the distance A of said gas distributor and said agitator paddle are 150 millimeters.
Above-mentioned cell culture reactor, said gas distributor are the stainless steel sintering net filter core that is welded on the said gas stream siphunculus.
Above-mentioned cell culture reactor, the filtering accuracy of said stainless steel sintering net filter core are 5 μ m～100 μ m.
Above-mentioned cell culture reactor, the length B of said gas distributor are 1/2nd to 2/3rds of said agitator paddle diameter D, and the distance A of said gas distributor and said agitator paddle is 100 millimeters～200 millimeters.
Cell culture reactor of the present invention has following beneficial technical effects: (1) is equipped with gas distributor because cell culture reactor of the present invention closes at said gas stream siphunculus on the end of said tank base; Make gas in the gas stream siphunculus when gas distributor gets into cell cultures liquid; Reduced the probability that produces bubble; Thereby reduce because the influence that the collapse of bubbles pair cell is caused improves the cell density in the reactor drum.(2) in the present invention; The center of said gas distributor is on the medullary ray of said whisking appliance; The axis of said tank body and the medullary ray of the said whisking appliance angle C between the two is 10～20 degree; The length B of said gas distributor is 1/2nd to 2/3rds of said agitator paddle diameter D, and the distance A of said gas distributor and said agitator paddle is 100 millimeters～200 millimeters; Thereby guarantee to match from gas and said whisking appliance waveform that said gas distributor comes out; Gas is distributed to pot liquid with liquid agitation waveform in the said tank body makes gas be fully used everywhere; And can be diffused into equably the said tank body from the gas that said gas distributor comes out, help the growth of tank body inner cell; The axis of preferred said tank body and the medullary ray of the said whisking appliance angle C between the two is 15 degree; The length B of said gas distributor is 1/2nd of said agitator paddle diameter D, and the distance A of said gas distributor and said agitator paddle is 150 millimeters; Gas that said gas distributor comes out and said whisking appliance waveform can coincide better; Gas can be distributed to pot liquid everywhere better with liquid agitation waveform in the said tank body; Gas can access more fully and utilize; Can be diffused into the gas that comes out from said gas distributor equably the said tank body better, help the growth of cell more.(3) said gas distributor is a stainless steel sintering net filter core, can be according to the stainless steel sintering net filter core of the different filtering accuracies of the different choice of want culturing cell.
Description of drawings
Fig. 1 is the structural representation of cell culture reactor of the present invention, wherein:
1-gas communication interface tube, 2-gas distributor, 3-whisking appliance, 4-bottom valve, 5-tank body, 6-gas stream siphunculus, 7-axis, 8-medullary ray.
Embodiment
The cell culture reactor of present embodiment comprises tank body 5, also comprises the deep ventilation module that is arranged in the said tank body 5, and is arranged on whisking appliance 3 and bottom valve 4 in said tank body 5 bottoms.Said deep ventilation module comprises gas stream siphunculus 6 and gas distributor 2, and said gas distributor 2 is installed on the end that said gas stream siphunculus 6 closes on said tank body 5 bottoms, and the other end of said gas stream siphunculus 6 is connected with air feeder.In the present embodiment, said gas distributor 2 is for to be welded on the stainless steel sintering net filter core on the said gas stream siphunculus 6, and the filtering accuracy of said stainless steel sintering net filter core is 20 μ m.Gas communication interface tube 1 designs according to reactor drum, can select modes such as flange, clip connect soon.The center of said gas distributor 2 is on the medullary ray 8 of said whisking appliance 3.The axis 7 of said tank body 5 and the medullary ray of the said whisking appliance 38 angle C between the two is 15 degree.The length B of said gas distributor 2 is 1/2nd of said whisking appliance 3 impeller diameter D.Said gas distributor 2 is 150 millimeters with the distance A of said whisking appliance 3 impellers.
In the present embodiment; Owing on the end that closes on said tank body 5 bottoms at gas stream siphunculus 6 stainless steel sintering net filter core being installed; Make gas be divided into various tiny air-flows and incorporate in the liquid, when the gas in gas stream siphunculus 6 gets into cell cultures liquid through stainless steel sintering net filter core, greatly reduced the probability that produces bubble through silk screen; Thereby reduce because the influence that the collapse of bubbles pair cell is caused improves the cell density in the reactor drum.In addition; The axis of the said tank body 5 of present embodiment cell culture reactor and the medullary ray of the said whisking appliance angle C between the two is 15 degree; The length B of said gas distributor 2 is 1/2nd of said whisking appliance 3 impeller diameter D, and the distance A of said gas distributor 2 and said whisking appliance 3 impellers is 150 millimeters; The gas that said gas distributor 2 comes out and the stirring waveform of said whisking appliance 3 can coincide better; Gas can be distributed to pot liquid everywhere better with liquid agitation waveform in the said tank body 5; Gas can access more fully and utilize; Make it possible to be diffused into the gas that comes out from said gas distributor 2 equably the said tank body 5 better, help the growth of cell more, can improve the cell density in the reactor drum better.
In other embodiment; The axis 7 of said tank body 5 and the medullary ray of the said whisking appliance 38 angle C between the two can be for being any number between 10～20 degree; The length B of said gas distributor 2 can for said whisking appliance 3 impeller diameter D 1/2nd to 2/3rds between any number, the distance A of said gas distributor 2 and said whisking appliance 3 impellers can be any number between 100 millimeters～200 millimeters; All can guarantee to match from gas and said whisking appliance waveform that said gas distributor comes out; Gas is distributed to pot liquid with liquid agitation waveform in the said tank body makes gas be fully used everywhere; And all can make the gas ratio of coming out be diffused into more equably the said tank body 5, help the growth of tank body 5 inner cells from said gas distributor 2; Simultaneously, said gas distributor 2 can be 100 millimeters～200 millimeters with the distance A of said whisking appliance 3 impellers, has also guaranteed the safety of said whisking appliance 3 impellers in whipping process.Different choice according to want culturing cell is different, can select the cultivation of the different stainless steel sintering net filter core of filtering accuracy with more favourable cell.
Claims (6)
1. cell culture reactor; Comprise tank body (5); It is characterized in that also comprise the deep ventilation module that is arranged in the said tank body (5), said deep ventilation module comprises gas stream siphunculus (6) and gas distributor (2); Said gas distributor (2) is installed on the end that said gas stream siphunculus (6) closes on said tank body (5) bottom, and the other end of said gas stream siphunculus (6) is connected with air feeder; The below of said gas distributor (2) is provided with whisking appliance (3); The axis (7) of said tank body (5) and the medullary ray (8) of said whisking appliance (3) the angle C between the two is 10～20 degree; The length B of said gas distributor (2) is 1/2nd to 2/3rds of said whisking appliance (3) impeller diameter D, and said gas distributor (2) is 100 millimeters～200 millimeters with the distance A of said whisking appliance (3) impeller.
2. cell culture reactor according to claim 1 is characterized in that, the center of said gas distributor (2) is on the medullary ray (8) of said whisking appliance (3).
3. cell culture reactor according to claim 1 is characterized in that, the axis (7) of said tank body (5) and the medullary ray (8) of said whisking appliance (3) the angle C between the two is 15 degree.
4. cell culture reactor according to claim 1 is characterized in that, said gas distributor (2) is 150 millimeters with the distance A of said whisking appliance (3) impeller.
5. cell culture reactor according to claim 1 is characterized in that, said gas distributor (2) is for being welded on the stainless steel sintering net filter core on the said gas stream siphunculus (6).
6. cell culture reactor according to claim 1 is characterized in that, the filtering accuracy of said stainless steel sintering net filter core is 5 μ m～100 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910210478 CN101709265B (en) | 2009-11-03 | 2009-11-03 | Cell culture reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910210478 CN101709265B (en) | 2009-11-03 | 2009-11-03 | Cell culture reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101709265A CN101709265A (en) | 2010-05-19 |
| CN101709265B true CN101709265B (en) | 2012-10-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910210478 Active CN101709265B (en) | 2009-11-03 | 2009-11-03 | Cell culture reactor |
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| CN (1) | CN101709265B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE738961C (en) * | 1940-11-11 | 1943-09-06 | Wilhelm Vogelbusch | Method and device for the fine ventilation of fluids, in particular of gas fluids |
| CH559060A5 (en) * | 1973-12-21 | 1975-02-28 | Mueller Hans Maennedorf | Aerator for aerobic fermentation - permits larger amts. of gas to be dispersed than previously |
| CN1587371A (en) * | 2004-07-05 | 2005-03-02 | 陶德录 | Magnetic floating type fermenting tank and its parallel fermenting process |
| CN201270799Y (en) * | 2008-09-24 | 2009-07-15 | 陈镜钊 | Rubber cover for clothing fabrication processing |
| CN201581081U (en) * | 2009-11-03 | 2010-09-15 | 金宇保灵生物药品有限公司 | Cell culture reactor |
-
2009
- 2009-11-03 CN CN 200910210478 patent/CN101709265B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE738961C (en) * | 1940-11-11 | 1943-09-06 | Wilhelm Vogelbusch | Method and device for the fine ventilation of fluids, in particular of gas fluids |
| CH559060A5 (en) * | 1973-12-21 | 1975-02-28 | Mueller Hans Maennedorf | Aerator for aerobic fermentation - permits larger amts. of gas to be dispersed than previously |
| CN1587371A (en) * | 2004-07-05 | 2005-03-02 | 陶德录 | Magnetic floating type fermenting tank and its parallel fermenting process |
| CN201270799Y (en) * | 2008-09-24 | 2009-07-15 | 陈镜钊 | Rubber cover for clothing fabrication processing |
| CN201581081U (en) * | 2009-11-03 | 2010-09-15 | 金宇保灵生物药品有限公司 | Cell culture reactor |
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| Publication number | Publication date |
|---|---|
| CN101709265A (en) | 2010-05-19 |
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