CN101709093B - Method for preparing blumea riparia water-soluble polysaccharides - Google Patents
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Abstract
The invention discloses a method for preparing blumea riparia water-soluble polysaccharides. One method of the invention comprises the steps of extraction, film separation, protein removal, desalination, decoloration, refining and refining. The second method of the invention comprises the steps of extraction, film separation, protein removal, silica gel column chromatographic separation, decoloration, dialysis and refining. The method has the advantages of simple process flow, energy conservation, high product purity, stable quality and the like. The production of the invention particularly has significant hemostatic activity in blood coagulation recalcification tests and isolated uterine tests of mice.
Description
Technical field
The present invention relates to medical technical field, specifically is a kind of preparation method of blumea riparia water-soluble polysaccharides.
Background technology
Herba Blumeae Balsamiferae is a composite family Herba Blumeae Balsamiferae platymiscium, record in " Chinese medicine sea ", and the Herba Blumeae Balsamiferae meridian distribution of property and flavor: light, sweet flat, go into liver, kidney, bladder three warps; Effect cures mainly: the sweet liver of fading in of promoting blood circulation and hemostasis-this article, and nourishing the liver is invigorated blood circulation, matter is puckery, and astringing to arrest bleeding has the merit that the stasis of blood is not stayed in invigorate blood circulation not blood trouble, hemostasis, can be used for various hemorrhages; Mention also in " Xinhua book on Chinese herbal medicine outline " that it is invigorated blood circulation, hemostatic function, can be used for menstrual period in advance, gynecological and hemorrhage symptoms such as massive postpartum vaginal bleeding.
Herba Blumeae Balsamiferae is all taken with the decocting decoction among the people as the existing long history of Guangxi medication among the people, is used to treat women's dysfunctional uterine hemorrhage, and diseases such as postpartum hemorrhage have unique curative effect.Chinese Traditional Medicines with the Herba Blumeae Balsamiferae exploitation mainly are the FUXUEKANG particles that Nanning Gui Xi pharmaceutical factory produces at present, medicines such as FUXUEKANG capsule.Therefore, Herba Blumeae Balsamiferae is a herbal medicine among the people that has value of exploiting and utilizing.The experts and scholars of domestic each ambit have launched number of research projects to contained chemical ingredients of this plant and drug effect.Stigmasterol, epifriedelinol, suberone, brown eleostearic acid ethyl ester, Ethyl Stearate, coffic acid, β-Gu Zaichun, tricin, tricin 7-O-β-D-glucopyranoside, apigenin, apigenin 7-O-β-D-glucopyranoside, Reseda odorata, luteolin 7-O-β-compounds such as D-glucopyranoside have therefrom been found.But the still rare both at home and abroad at present relevant RR of the concrete chemical ingredients of Herba Blumeae Balsamiferae aspect pharmacologically active.Particularly also very limited to the correlative study of the contained polysaccharose substance of Herba Blumeae Balsamiferae plant.
Various researchs show that glucide has various biological function, as having unique effects at aspect such as anticancer, antitumor, antiviral, anti-ageing, hypoglycemic.Because the biological activity of polysaccharide is very extensive and cytotoxic effect is little, aspect the multiple diseases such as cancer therapy, treating diabetes and anti-virus infection suitable notable therapeutic effect is being arranged.People are to polysaccharide, and particularly herbal polysaccharide has carried out deep research and application, and its biological significance has received extensive concern, become one of the world of medicine's hot fields.
Summary of the invention
The present invention provides a kind of preparation method who from the Herba Blumeae Balsamiferae plant, extracts blumea riparia water-soluble polysaccharides.So that the concrete chemical ingredients of clear and definite Herba Blumeae Balsamiferae performance anastalsis in diseases such as treatment women dysfunctional uterine hemorrhage, postpartum hemorrhage, for further research and the drug development utilization of Herba Blumeae Balsamiferae plant provides reference frame.
The present invention is following for the technical scheme that solves the problems of the technologies described above:
One of preparation method of blumea riparia water-soluble polysaccharides comprises the steps:
(1) extracts: each deionized water or the zero(ppm) water that adds 15 times of weight of the dried medicinal material of Herba Blumeae Balsamiferae is decocted, totally 2 times, each 3 hours, filter merging filtrate.
(2) membrane sepn: the filtrating that will extract is carried out micro-filtration and ultra-filtration and separation with hollow tubular ceramic microfiltration membrane and the organic ultra-filtration membrane of hollow tubular, obtains molecular weight ranges 10
3To 10
4Between parting liquid, this parting liquid is evaporated to about 0.1g/mL for 60 ℃ with Rotary Evaporators.
(3) deproteinated: under ice bath and agitation condition, adding weight concentration is 20% trichloroacetic acid solution, makes the weight concentration of trichoroacetic acid(TCA) in the polysaccharide soln system reach 5%, centrifugal removal vegetable-protein deposition after the hold over night.
(4) desalination: the supernatant after centrifugal is neutralized to pH with the NaOH solution of 1mol/L and equals 7; Be evaporated to about 0.2～0.5g/mL, be cooled to and add that concentration of ethanol is 75～80% in absolute ethyl alcohol to the solution after the room temperature, in 5 ℃ of refrigerators, leave standstill and make salt deposition, spinning remove sedimentary trichoroacetic acid(TCA) to receive salt; After the supernatant evaporated under reduced pressure removed ethanol, with 100mL deionized water or dissolved in distilled water.
(5) decolouring: with the polysaccharide soln behind above-mentioned desalination and the removal ethanol; Separate with the decolouring of DEAE Anion exchange column chromatography,, collect sugary soln with deionized water wash-out polysaccharide; Be evaporated to sugared content 0.5g/mL, get the pale brown look cotton-shaped Crude polysaccharides of preliminary purification through lyophilize.
(6) refining: as the Crude polysaccharides of (5) to be dissolved in the 10mL deionized water, behind 0.45 μ m micropore filtering film filtration under diminished pressure, to cross Sephadex G-10 gel chromatography separation and remove remaining salt and other small molecular weight impurities; Utilize Sephadex G-50 gel chromatography to carry out purifying again; With the ultrapure water is moving phase, quantitatively receives through automatic part receptor, and UV spectrum detects; Merge unimodal part solution; This solution decompression is concentrated into sugared content 0.5～1.0g/mL, through lyophilize, obtain the purified blumea riparia water-soluble polysaccharides.
The preparing method's of blumea riparia water-soluble polysaccharides two comprises the steps:
(1) extracts: each deionized water or the zero(ppm) water that adds 10 times of weight of the dried medicinal material of Herba Blumeae Balsamiferae is decocted, totally 3 times, each 2 hours, filter merging filtrate.
(2) membrane sepn: the filtrating that will extract is carried out micro-filtration and ultra-filtration and separation with hollow tubular ceramic microfiltration membrane and the organic ultra-filtration membrane of hollow tubular, obtains molecular weight ranges 10
3To 10
4Between parting liquid, this parting liquid is evaporated to about 0.2g/mL.
(3) deproteinated: under condition of ice bath, adding concentration while stirring is 20% trichloroacetic acid solution, makes the concentration of trichoroacetic acid(TCA) reach 5%, centrifugal removal vegetable-protein deposition after the hold over night in 5 ℃ of refrigerators; Supernatant is neutralized to pH with the NaOH solution of 1mol/L and equals 7, and the concentrating under reduced pressure after-filtration is removed sedimentary salinity.
(4) silica gel column chromatography separates: with the about 0.5g/mL of polysaccharide enriching soln behind the deproteinated., evenly admix 200 order silica gel evaporated under reduced pressure after, appearance is crossed silicagel column on the dry method, presses different ratios blended solution system gradient elution with methyl alcohol and ETHYLE ACETATE.Collection contains the sugar moieties elutriant, and evaporated under reduced pressure is also filtered with the water dissolution of about 50mL after having removed methyl alcohol and ETHYLE ACETATE, gets the pale brown look Crude polysaccharides of preliminary purification through lyophilize.
(5) decolouring: the deionized water that Crude polysaccharides is dissolved in 5 times of weight.Separate with the decolouring of DEAE Anion exchange column chromatography, with deionized water wash-out polysaccharide.Collect sugary soln, be evaporated to about 0.1g/mL.
(6) dialysis: with the polysaccharide liquid concentrator use molecular weight cut-off of decolouring is 10
3The dialysis tubing flowing water dialysis 48h of Da, deionized water dialysis 24h removes and desalts and small molecular weight impurity.Solution to 0.5 in the concentrating under reduced pressure dialysis tubing～1.0g/mL.
(7) refining: with the polysaccharide liquid concentrator with 0.45 μ m micropore filtering film filtration under diminished pressure after; Utilizing Sephadex G-50 gel chromatography to be further purified again, is moving phase with the ultrapure water, quantitatively receives through automatic part receptor; UV spectrum detects; Merge unimodal part solution, the concentrating under reduced pressure postlyophilization obtains the purified blumea riparia water-soluble polysaccharides.
One of above-mentioned preparation method and preparation method two in, said step (2) membrane sepn: used membrane separation plant is that the outstanding film engineering of Hefei generation Ltd produces, and the molecular weight cut-off of ceramic microfiltration membrane is 10
5Da, the molecular weight cut-off of organic ultra-filtration membrane is respectively 10
4Da and 10
3Da.
One of above-mentioned preparation method and preparation method two in, said concentrating under reduced pressure all utilizes Rotary Evaporators to concentrate not being higher than under 60 ℃ of conditions reduction vaporization.
Being characterized as of the blumea riparia water-soluble polysaccharides of one of above-mentioned preparation method and preparing method's two preparations: (a) Herba Blumeae Balsamiferae polysaccharide outward appearance is a white cotton shape solid; Be the simple component of MWD homogeneous, its number-average molecular weight and weight-average molecular weight are respectively 2654 and 2716; (b) blumea riparia water-soluble polysaccharides is made up of seminose and fructose.
The blumea riparia water-soluble polysaccharides of one of above-mentioned preparation method and preparing method's two preparations has hemostatic function, can be used for treating diseases such as women's dysfunctional uterine hemorrhage, postpartum hemorrhage.
Advantage of the present invention is:
1. the present invention is used in combination method separation and purification water-soluble polysaccharides such as membrane sepn, silica gel column chromatography and gel column chromatography, has advantages such as technical process is simple, energy efficient.
2. product purity is higher, steady quality; Particularly in multiple calcium experiment of blood coagulation and the experiment of small white mouse isolated uterine, possesses significant styptic activity effect.
Description of drawings
Fig. 1 is the GPC gel permeation chromatography figure of blumea riparia water-soluble polysaccharides of the present invention.
Fig. 2 is the uv-spectrogram of blumea riparia water-soluble polysaccharides of the present invention.
Fig. 3 is the infared spectrum of blumea riparia water-soluble polysaccharides of the present invention.
Fig. 4 is the performance liquid chromatography of blumea riparia water-soluble polysaccharides of the present invention.
Fig. 5 is the performance liquid chromatography of standard monose.
Embodiment
Composition analysis to blumea riparia water-soluble polysaccharides of the present invention:
1. blumea riparia water-soluble polysaccharides MWD test
Blumea riparia water-soluble polysaccharides is adopted gel permeation chromatography (GPC) analysis, as. shown in Figure 1, single symmetrical peak type shows that this polysaccharide is the simple component of MWD homogeneous, uses the polysaccharide of known molecular amount to be standard specimen, and sets up the GPC calibration curve.The number-average molecular weight and the weight-average molecular weight of trying to achieve this polysaccharide according to the standard correction curve are respectively 2654 and 2716.
2. UV spectrum detects
Blumea riparia water-soluble polysaccharides is carried out UV spectrum to be detected: get the about 1mg of Herba Blumeae Balsamiferae polysaccharide, add suitable quantity of water and dissolve, in wavelength 200～600nm scope, scan, with deionized water as blank.There is not absorption through UV scanning 280nm place, as shown in Figure 2, confirm not contain polypeptide, protein.
3. ir spectra detects
Blumea riparia water-soluble polysaccharides is carried out ir spectra to be detected: get the about 2mg of purifying exsiccant blumea riparia water-soluble polysaccharides sample, add an amount of KBr and be ground to carefully, compressing tablet is measured ir spectra.As shown in Figure 3, collection of illustrative plates shows that blumea riparia water-soluble polysaccharides is at 4000～500cm
-1The charateristic avsorption band that has β-furans glucide in the scope.
4. monose compositional analysis
Blumea riparia water-soluble polysaccharides is carried out the monose compositional analysis: blumea riparia water-soluble polysaccharides utilizes efficient liquid phase chromatographic analysis as shown in Figure 4 after the sulfuric acid complete hydrolysis.Through contrasting with monose mark article such as D-glucose, D-seminose, D-fructose, L-rhamnosyls, as shown in Figure 5, confirm that blumea riparia water-soluble polysaccharides is made up of fructose and seminose.
Below in conjunction with real two embodiment of the present invention the present invention is elaborated.But need to prove that embodiment does not constitute the restriction that the present invention is required protection domain.
Blumea riparia water-soluble polysaccharides preparation method one, operation steps are following successively:
1. extract: the each deionized water that adds 15 times of weight of the dried medicinal material of 3.0kg Herba Blumeae Balsamiferae is decocted 2 times, each 3 hours, filter merging filtrate.
2. membrane sepn: utilize membrane separation plant to carry out rough segmentation, the filtrating that step 1 is extracted molecular weight cut-off be 10
5The ceramic membrane of Da, molecular weight is less than 10
5The filtrating of Da is 10 through molecular weight cut-off
4The organic membrane of Da, it is 10 that filtrating is continued through molecular weight cut-off
3The organic membrane of Da obtains molecular weight ranges 10
3To 10
4Between trapped fluid, be evaporated to 500mL.
(3) deproteinated: under ice bath and agitation condition, add weight concentration and be 20% the about 170mL of trichloroacetic acid solution, make that the trichoroacetic acid(TCA) weight concentration is the sedimentary vegetable-protein of centrifugal removal after 5%, 5 ℃ of refrigerator hold over night in the solution system.
4. desalination: the supernatant behind the deproteinated is neutralized to the pH value with the NaOH solution of 1mol/L and equals 7; Concentrate polysaccharide soln to sugared content 0.5g/mL; Be cooled to the alcohol concn that adds absolute ethyl alcohol to solution after the room temperature and be about 75～80%; Make trichoroacetic acid(TCA) sodium salt deposition, leave standstill 12h, centrifugally remove sedimentary salt in 5 ℃ of refrigerators.After the supernatant evaporated under reduced pressure removed ethanol, with 100mL deionized water or dissolved in distilled water.
5. decolouring: the polysaccharide soln behind above-mentioned desalination and the removal ethanol is decoloured with the DEAE Anion exchange column chromatography.With deionized water wash-out polysaccharide component.Be evaporated to the about 0.5g/mL of sugared content.Get the light yellow cotton-shaped Crude polysaccharides of 4g preliminary purification through lyophilize.
6. refining: will decolouring completely, Crude polysaccharides is dissolved in about 10mL deionized water; Behind the micropore filtering film filtration under diminished pressure with 0.45 μ m, cross the SephadexG-10 gel column chromatography, separate and remove remaining salt and other small molecular weight impurities; Utilize Sephadex G-50 gel chromatography to carry out purifying again; With the ultrapure water is moving phase, quantitatively receives through automatic part receptor, and UV spectrum detects.Merge unimodal part solution, lyophilize gets the purified blumea riparia water-soluble polysaccharides of 2.70g.
Blumea riparia water-soluble polysaccharides preparation method two, operation steps are following successively:
1. extract: the each zero(ppm) water that adds 10 times of weight of the dried medicinal material of 3.0kg Herba Blumeae Balsamiferae is decocted 3 times, each 2 hours, filter merging filtrate.
2. membrane sepn: utilize membrane separation plant to carry out rough segmentation, the filtrating that step 1 is extracted molecular weight cut-off be 10
5The ceramic membrane of Da, molecular weight is less than 10
5The filtrating of Da is 10 through molecular weight cut-off
4The organic membrane of Da, it is 10 that filtrating is continued through molecular weight cut-off
3The organic membrane of Da obtains molecular weight ranges 10
3To 10
4Between trapped fluid, be evaporated to 500mL.
3. deproteinated: under condition of ice bath, adding weight concentration while stirring is the about 170mL of 20% trichloroacetic acid solution, makes the weight concentration of trichoroacetic acid(TCA) reach 5%, the sedimentary vegetable-protein of centrifugal removal after the refrigerator hold over night.Supernatant is concentrated into the about 0.2 gram g/mL after-filtration of 50mL and removes sedimentary salinity with the NaOH solution neutralization of 1mol/L.
4. silica gel column chromatography separates: with the about 0.5g/mL of Deproteinated polysaccharide liquid concentrator; Evenly admix 100g silica gel (200 order) evaporated under reduced pressure; Appearance is crossed silicagel column on the dry method, presses 1: 4,2: 3,3: 2 and the mixed system gradient elution of 4: 1 different ratioss with methyl alcohol and ETHYLE ACETATE.Collection merges methyl alcohol, ETHYLE ACETATE is the sugary soln of 4: 1 moving phase wash-out, with about 50mL water dissolution and filtration, gets the pale brown look Crude polysaccharides of 6g preliminary purification through lyophilize behind evaporated under reduced pressure methyl alcohol and the ETHYLE ACETATE.
5. decolouring: Crude polysaccharides is dissolved in the 30mL deionized water, decolours with the DEAE Anion exchange column chromatography.With deionized water eluting water soluble polysaccharide.Collect sugary soln and be evaporated to 80mL.
6. dialysis: with the polysaccharide liquid concentrator use dialysis tubing molecular weight cut-off of decolouring is 10
3Da flowing water dialysis 48h, deionized water dialysis 24h, desalination and small molecular weight impurity.Solution decompression in the dialysis tubing is concentrated into 10mL.
7. refining: as with utilizing Sephadex G-50 gel chromatography to be further purified again behind the micropore filtering film filtration under diminished pressure of polysaccharide liquid concentrator with 0.45 μ m, to be moving phase with the ultrapure water, quantitatively to receive that UV spectrum detects through automatic part receptor.Merge unimodal part solution, concentrate postlyophilization, obtain the purified blumea riparia water-soluble polysaccharides of 3.15g.
Claims (6)
1. the preparation method of a blumea riparia water-soluble polysaccharides is characterized in that comprising the steps:
(1) extracts: each deionized water or the zero(ppm) water that adds 15 times of weight of the dried medicinal material of Herba Blumeae Balsamiferae is decocted, totally 2 times, each 3 hours, filter merging filtrate;
(2) membrane sepn: the filtrating that will extract is carried out micro-filtration and ultra-filtration and separation with hollow tubular ceramic microfiltration membrane and the organic ultra-filtration membrane of hollow tubular, obtains molecular weight ranges 10
3To 10
4Between parting liquid, this parting liquid is evaporated to 0.1g/mL for 60 ℃ with Rotary Evaporators;
(3) deproteinated: under ice bath and agitation condition, the adding weight concentration is 20% trichloroacetic acid solution, makes the weight concentration of trichoroacetic acid(TCA) in the polysaccharide soln system reach 5%, centrifugal removal vegetable-protein deposition after the hold over night;
(4) desalination: the supernatant after centrifugal is neutralized to pH with the NaOH solution of 1mol/L and equals 7; Be evaporated to (0.2～0.5) g/mL, be cooled to and add that concentration of ethanol is 75～80% in absolute ethyl alcohol to the solution after the room temperature, in 5 ℃ of refrigerators, leave standstill to make the salt deposition, sedimentary trichoroacetic acid(TCA) sodium salt is removed in spinning; After the supernatant evaporated under reduced pressure removed ethanol, with 100mL deionized water or dissolved in distilled water;
(5) decolouring: with the polysaccharide soln behind above-mentioned desalination and the removal ethanol; Separate with the decolouring of DEAE Anion exchange column chromatography,, collect sugary soln with deionized water wash-out polysaccharide; Be evaporated to sugared content 0.5g/mL, get the pale brown look cotton-shaped Crude polysaccharides of preliminary purification through lyophilize;
(6) refining: as the Crude polysaccharides of (5) to be dissolved in the 10mL deionized water, behind 0.45 μ m micropore filtering film filtration under diminished pressure, to cross Sephadex G-10 gel chromatography separation and remove remaining salt and other small molecular weight impurities; Utilize Sephadex G-50 gel chromatography to carry out purifying again; With the ultrapure water is moving phase, quantitatively receives through automatic part receptor, and UV spectrum detects; Merge unimodal part solution; This solution decompression is concentrated into sugared content 0.5～1.0g/mL, through lyophilize, obtain the purified blumea riparia water-soluble polysaccharides.
2. the preparation method of a blumea riparia water-soluble polysaccharides is characterized in that comprising the steps:
(1) extracts: each deionized water or the zero(ppm) water that adds 10 times of weight of the dried medicinal material of Herba Blumeae Balsamiferae is decocted, totally 3 times, each 2 hours, filter merging filtrate;
(2) membrane sepn: the filtrating that will extract is carried out micro-filtration and ultra-filtration and separation with hollow tubular ceramic microfiltration membrane and the organic ultra-filtration membrane of hollow tubular, obtains molecular weight ranges 10
3To 10
4Between parting liquid, this parting liquid is evaporated to 0.2g/mL;
(3) deproteinated: under condition of ice bath, adding mass concentration while stirring is 20% trichloroacetic acid solution, makes the concentration of trichoroacetic acid(TCA) reach 5%, centrifugal removal vegetable-protein deposition after the hold over night in 5 ℃ of refrigerators; Supernatant is neutralized to pH with the NaOH solution of 1mol/L and equals 7, and the concentrating under reduced pressure after-filtration is removed sedimentary salinity;
(4) silica gel column chromatography separates: with the about 0.5g/mL of polysaccharide enriching soln behind the deproteinated; After evenly admixing 200 purpose silica gel evaporated under reduced pressure; Appearance is crossed silicagel column on the dry method, presses different ratios blended solution system gradient elution with methyl alcohol and ETHYLE ACETATE, collects to contain the sugar moieties elutriant; Evaporated under reduced pressure is also filtered with the water dissolution of about 50mL after having removed methyl alcohol and ETHYLE ACETATE, gets the pale brown look Crude polysaccharides of preliminary purification through lyophilize;
(5) decolouring: Crude polysaccharides is dissolved in the deionized water of 5 times of weight, separates with the decolouring of DEAE Anion exchange column chromatography, with deionized water wash-out polysaccharide, the collection sugary soln is evaporated to about 0.1g/mL;
(6) dialysis: with the polysaccharide liquid concentrator use molecular weight cut-off of decolouring is 10
3The dialysis tubing flowing water dialysis 48h of Da, deionized water dialysis 24h removes and desalts and small molecular weight impurity, solution to 0.5 in the concentrating under reduced pressure dialysis tubing～1.0g/mL;
(7) refining: with the polysaccharide liquid concentrator with 0.45 μ m micropore filtering film filtration under diminished pressure after; Utilizing Sephadex G-50 gel chromatography to be further purified again, is moving phase with the ultrapure water, quantitatively receives through automatic part receptor; UV spectrum detects; Merge unimodal part solution, the concentrating under reduced pressure postlyophilization obtains the purified blumea riparia water-soluble polysaccharides.
3. according to the preparation method of claim 1 or the said a kind of blumea riparia water-soluble polysaccharides of claim 2, it is characterized in that said step (2) membrane sepn: the molecular weight cut-off of used ceramic microfiltration membrane is 10
5Da, the molecular weight cut-off of organic ultra-filtration membrane is respectively 10
4Da and 10
3Da.
4. according to the preparing method's of one of the preparation method of the said a kind of blumea riparia water-soluble polysaccharides of claim 1 or the said a kind of blumea riparia water-soluble polysaccharides of claim 2 two; It is characterized in that said concentrating under reduced pressure all utilizes Rotary Evaporators to concentrate not being higher than under 60 ℃ of conditions reduction vaporization.
5. according to the preparation method of claim 1 or the described a kind of blumea riparia water-soluble polysaccharides of claim 2; It is characterized in that; Being characterized as of the blumea riparia water-soluble polysaccharides of this method preparation: (a) Herba Blumeae Balsamiferae polysaccharide outward appearance is a white cotton shape solid; Be the simple component of MWD homogeneous, its number-average molecular weight and weight-average molecular weight are respectively 2654 and 2716; (b) blumea riparia water-soluble polysaccharides is made up of seminose and fructose.
6. according to the preparation method of claim 1 or the described a kind of blumea riparia water-soluble polysaccharides of claim 2; It is characterized in that; The blumea riparia water-soluble polysaccharides of this method preparation has hemostatic function, can be used for treating women's dysfunctional uterine hemorrhage, postpartum hemorrhage disease.
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| CN102512344B (en) * | 2011-12-28 | 2013-06-05 | 中国热带农业科学院热带作物品种资源研究所 | Blumea balsamifera extract, preparation method and application thereof in oral care and clean product |
| CN107475215A (en) * | 2017-09-06 | 2017-12-15 | 成都晟博源生物工程有限公司 | A kind of extracting method of phosphorylase B |
| CN109627352B (en) * | 2018-11-26 | 2021-06-11 | 吉林化工学院 | Decolouring process of corn stigma polysaccharide |
| CN113082068B (en) * | 2021-05-13 | 2022-10-21 | 广西壮族自治区食品药品检验所 | Method for extracting blumea riparia active extract and application of blumea riparia active extract in preparing medicine for treating dysfunctional uterine bleeding |
| CN113801798B (en) * | 2021-08-02 | 2023-07-28 | 广西大学 | Acidocella adephagia strain A50, extracellular polysaccharide produced by same and application thereof |
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| CN101181329A (en) * | 2007-11-28 | 2008-05-21 | 广西万寿堂药业有限公司 | Total chromocor extract of blumea riparia as well as pharmaceutical composition and preparing method thereof |
| CN101199571A (en) * | 2007-11-30 | 2008-06-18 | 广西万寿堂药业有限公司 | Yunnan and Guangxi Einar sweet syrup preparing method and detective method thereof |
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| CN101181329A (en) * | 2007-11-28 | 2008-05-21 | 广西万寿堂药业有限公司 | Total chromocor extract of blumea riparia as well as pharmaceutical composition and preparing method thereof |
| CN101199571A (en) * | 2007-11-30 | 2008-06-18 | 广西万寿堂药业有限公司 | Yunnan and Guangxi Einar sweet syrup preparing method and detective method thereof |
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