CN101703238B - Sea-cucumber chitosan capsules and preparation method thereof - Google Patents

Abstract

The invention relates to sea-cucumber chitosan capsules and a preparation method thereof. The preparation method is realized in a way that: sea cucumbers are pulverized until the granularity reaches 200 mu m-100 nm; the chitosan is chitosan powder the deacetylation degree of which is greater than 70% and the granularity of which is 200 mu m-100 nm; the sea cucumber powder and the chitosan powder are evenly mixed in the mass ratio of 5:5-9:1; and each capsule is filled with 0.3-0.6 g of the sea cucumber powder and the chitosan powder which are mixed proportionally and evenly. The animal experiment of anticoagulant activity indicates that under the condition of the same intragastrical dose for mice, the anticoagulant activities of the sea-cucumber chitosan powder and the sea cucumber powder respectively extend the anticoagulant activity by RT 988.68% and 1381.63%, which means the anticoagulant activity B is smaller than A. Compared with physiological saline, P is smaller than 0.001, and the difference is quite obvious. The invention reasonably combines the sea cucumbers with sorbefacient chitosan having health care value, so that biomacromolecules of the sea cucumbers can be well absorbed by human bodies.

Description

Sea-cucumber chitosan capsules and preparation method thereof

Technical field

The invention belongs to and derive from molluscan food processing field, relate to the formulation art of Medicines and Health Product simultaneously.

Background technology

Shitosan is also referred to as chitosan, crust amine, chitin etc., is chitinous deacetylation product.Be described as by people " the 6th vital principle ".Scientific and rational edible, can in the physiological activity of human body, play important effect.Shitosan is positively charged cation food fiber; It is again the alkaline polysaccharide of the unique existence of nature; Thereby determined it to have important human body physiological function, be called as the functional food active factors of two Pius XIs circle, the physiological activity of human body has been played many-sided regulating action.At the medicines and health protection field of food, the physiological hygiene function of shitosan demonstrates variation, has to improve immunity, activating cell, prophylaxis of cancer, reduction blood fat, brings high blood pressure down, regulates blood sugar, delays senility, regulates multiple biological function such as organismic internal environment balance.Simultaneously, shitosan is because its natural characteristic etc., and shitosan and derivative etc. thereof is to study more bioadhesive polymers in recent years, as intestinal absorption promoter, is mainly used in the oral absorption of improving macromolecular drug.The bioadhesive of this base polymer is strong and safe, and it is had a extensive future.

Sea cucumber is the generally acknowledged functional health good merchantable brand of of China, has shown extremely strong activity in anti-freezing, anti-bolt, diabetes, anticancer, prostate, cardiovascular and cerebrovascular disease or the like many aspects, so sea cucumber is as daily health products, can be flourishing long time.But the health care products product of present sea cucumber, great majority also all are confined on holothurian freeze-dried powder and the beche-de-mer capsules series products, and these products but are to have played positive facilitation for health care.But sea cucumber is in view of its constituent, and great majority all belong to large biological molecule, sea cucumber polysaccharide etc. especially, and the human body oral absorption has certain degree of difficulty.The sea cucumber resource scarcity costs an arm and a leg, therefore how can better utilization sea cucumber resource, give full play to the health-care effect of sea cucumber, and be only the good thing of benifit for people and nation.We know, oral Preparation desire performance general action, and absorption is crucial.In order to improve the bioavilability of oral drug preparation, usually use sorbefacient.Shitosan and derivative thereof with the positive charge group can interact with electronegative serine group in the cell membrane protein, open tight connection to invertibity and increase parietal cell and transport.Thereby promoted to be difficult to the absorption efficiency of the macromolecular substances of oral absorption.

Sea cucumber and shitosan cooperated process a kind of health products, can better bring into play its health-care effect.But still there are not at present the appearance of this series products or report for information about.

Summary of the invention

The purpose of this invention is to provide the health-caring capsule product that a kind of sea cucumber and shitosan combine.Its preparation method is:

1. raw material sea cucumber: various sea cucumbers all can, grinding particle size is 200 μ m～100nm.

2. shitosan is a deacetylation greater than 70% shitosan powder, and grinding particle size is 200 μ m～100nm.

3. its mass ratio is: the sea cucumber powder: chitosan powder=5: 5～9: 1,

4. above-mentioned sea cucumber powder and the chitosan powder that mixes in proportion of the 0.3～0.6g that packs in every capsule.

In addition, capsule 's content can also prepare with following method:

With shitosan according to 1: 5～1: 20 (shitosan: after ratio water) adds entry; Use again concentration be the hydrochloric acid solution of 0.1～0.5mol/L to be adjusted to pH be 1～7, after the stirring and dissolving, add the sea cucumber powder according to 5: 5～1: 9 ratio with cucumber product; Even matter, freeze-drying forms.Right rear packing.

The present invention carries out the animal experiment of anticoagulating active to sea cucumber powder and sea cucumber and shitosan mixed powder (being capsule 's content of the present invention).Consequently: to small white mouse, sea cucumber chitosan powder and sea cucumber powder prolong RT 988.68% and 1381.63% respectively under same dose filling stomach situation, i.e. anticoagulating active B＜A.P＜0.001 of comparing with physiological saline has very significant difference.

Therefore the present invention provide a kind of with sea cucumber with have the sorbefacient shitosan that health care is worth and carry out reasonable combination; Make the large biological molecule of sea cucumber better to be absorbed by the body; The scientific combination of sea cucumber and shitosan simultaneously, the health care value of new health product is stronger.

The specific embodiment

Instance 1

1, get fresh and alive sea cucumbers, subsequent use after the cleaning and removing impurities.

2, it is subsequent use greater than 70% food-grade shitosan to get deacetylation.

3, sea cucumber is carried out drying, pulverize, subsequent use.The fineness of pulverizing is 200 μ m.

4, shitosan is dry, pulverize, subsequent use.The fineness 200 μ m that pulverize.

5, proportionally mix at 5: 53 and 4.

6, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 2

1, get fresh and alive sea cucumbers, subsequent use after the cleaning and removing impurities.

2, it is subsequent use greater than 70% food-grade shitosan to get deacetylation.

3, sea cucumber is carried out drying, pulverize, subsequent use.The fineness of pulverizing is 100 μ m.

4, shitosan is dry, pulverize, subsequent use.The fineness 100 μ m that pulverize.

5, proportionally mix at 6: 43 and 4.

6, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 3

1, get fresh and alive sea cucumbers, subsequent use after the cleaning and removing impurities.

2, it is subsequent use greater than 90% food-grade shitosan to get deacetylation.

3, sea cucumber is carried out drying, pulverize, subsequent use.The fineness of pulverizing is 1000nm.

4, shitosan is dry, pulverize, subsequent use.The fineness 1000nm that pulverizes.

5, proportionally mix at 7: 33 and 4.

6, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 4

1, it is subsequent use greater than 98% food-grade shitosan to get deacetylation.

2, freeze-drying sea cucumber powder being crushed to fineness once more is 300nm.

3, shitosan is dry, pulverize, subsequent use.The fineness 300nm that pulverizes.

4, proportionally mix at 9: 12 and 3.

5, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 5

1, get deacetylation greater than 70% food-grade shitosan, be dried, be crushed to fineness 200 μ m, subsequent use.

2, freeze-drying sea cucumber powder being crushed to fineness once more is 200 μ m.

3, with shitosan according to 1: 5 (shitosan: after ratio water) adds entry, use again concentration as 0.5mol/L hydrochloric acid to pH be 1, after the stirring and dissolving, add the sea cucumber powder according to 5: 5 ratio with cucumber product, even matter, freeze-drying forms.

4, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 6

1, get deacetylation greater than 80% food-grade shitosan, be dried, be crushed to fineness 100 μ m, subsequent use.

2, freeze-drying sea cucumber powder being crushed to fineness once more is 100 μ m.

3, with shitosan according to 1: 8 (shitosan: after ratio water) added entry, using concentration to be adjusted to PH as the hydrochloric acid solution of 0.25mol/L again was 2, after the stirring and dissolving, added the sea cucumber powder with cucumber product according to 4: 6 ratio, even matter, freeze-drying forms.

4, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 7

1, get deacetylation greater than 90% food-grade shitosan, be dried, be crushed to fineness 1000nm, subsequent use.

2, freeze-drying sea cucumber powder being crushed to fineness once more is 1000nm.

3, with shitosan according to 1: 15 (shitosan: after ratio water) added entry, using concentration to be adjusted to pH as the hydrochloric acid solution of 0.25mol/L again was 4, after the stirring and dissolving, added the sea cucumber powder with cucumber product according to 3: 7 ratio, even matter, freeze-drying forms.

4, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Instance 8

1, get deacetylation greater than 98% food-grade shitosan, be dried, be crushed to fineness 100nm, subsequent use.

2, freeze-drying sea cucumber powder being crushed to fineness once more is 100nm.

3, with shitosan according to 1: 20 (shitosan: after ratio water) added entry, using concentration to be adjusted to pH as the hydrochloric acid solution of 0.1mol/L again was 6.9, after the stirring and dissolving, added the sea cucumber powder with cucumber product according to 2: 8 ratio, even matter, freeze-drying forms.

4, adorn capsule equipment dress capsule, every capsule 0.6g automatically.

Claims (2)

1. sea-cucumber chitosan capsules is characterized in that content is that granularity is the sea cucumber of 200 μ m～100nm and the mixture of shitosan, and the mass ratio of sea cucumber and shitosan is: 5:5～9:1.

2. the preparation method of sea-cucumber chitosan capsules is characterized in that processing step is:

(1) raw material sea cucumber: various sea cucumbers all can, grinding particle size is 200 μ m～100nm;

(2) shitosan is a deacetylation greater than 70% shitosan powder, and grinding particle size is 200 μ m～100nm;

(3) by mass ratio be: sea cucumber powder: chitosan powder=5:5～9:1 mixes;

(4) above-mentioned sea cucumber powder and the chitosan powder that mixes in proportion of 0.3～0.6g of packing in every capsule.