CN101698066B - Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof - Google Patents
Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof Download PDFInfo
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Abstract
The invention provides a Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, a preparation method thereof and applications of the Chinese medicine preparation in preparing medicines for treating urinary system calculi, urinary tract infection, cholecystitis, gall-stone and prostatitis. According to the theory of Chinese medicines, through the experiment and screening for many years, the invention reselects raw drugs in the prescription, optimizes the preparation technology, greatly enhances the medicinal functions of promoting diuresis for stranguria and removing heat for detumescence, has unexpected obvious curative effect on treating urinary system diseases, and especially produces unexpected effects of resisting bacteria, resisting prostatitis, relieving pains and benefiting gallbladder which are not provided in the prior art. The Chinese medicine preparation of the invention exerts the traditional advantages of Chinese medicines, takes effect quickly, obviously improves the subjective symptom and the physical sign, and does not have obvious side effect.
Description
Technical field
The present invention relates to Chinese medicine preparation of a kind of antiinflammatory, pain relieving, antibiotic, calculus, function of gallbladder promoting and preparation method thereof, with and application in the medicine of preparation treatment urinary system calculus and urinary tract infection.
Background technology
Diseases of urinary system is human common disease, its cardinal symptom have frequent micturition short red, urinate puckery causalgia, serious occurred heating, thirsty, abdominal pain, waist is ached, hematuria, suppurate, constipation with dry stool or completely mashed, its reason has following several, 1, renal calculus or urinary tract, vesical calculus.Owing to calculus has been blocked kidney, urinary tract, bladder and is made urinary tract not smooth, make waist or abdominal part continue dull pain or paroxysmal pain, often be radiated to hypogastric region or cloudy down, can accompany phenomenons such as hematuria, X line plain film or radiography can show the calculus shade, and B ultrasonic also can reflect existence and big or small, the position of calculus; 2, pyelonephritis, cystitis, urethritis are mainly caused by bacterial infection or local patholoic change, when the urinary system acute inflammation, the urethral orifice redness often occurs, and purulent secretion is arranged, and the urine spectroscopy has red white corpuscle or pus cell, the urine positive bacterial culture; 3, the urinary tract infection that causes of sexually transmitted disease (STD) mainly is that patient has bad sexual behaviour and causes infecting gonococcus, non-gonococcal infection, and frequent micturition, urgent micturition, pudendum redness also appear in its symptom, and purulent secretion is arranged, situations such as hematuria.At present, be surgical calculus removing and external instrument concussion rubble calculus method to kidney, urinary tract, cystolithic western medicine therapy, operate on modus operandi expense height, patient's misery and postoperative recurrence rate height; Though it is fast that calculus method speed is impacted in instrument concussion, short treating period, the equipment manufacturing cost height is not suitable for the extensive patients in samll cities and towns or rural area.The traditional Chinese medical science then is to adopt various calculus prescriptions, and its cost is lower, and also each is variant for treating both the principal and secondary aspects of a disease, but most prescription among the people does not pass through strict pharmacodynamic experiment and toxicity test, therapeutic effect.At present, the Therapeutic Method of the disease doctor trained in Western medicine that acute pyelonephritis, cystitis, urethritis and sexually transmitted disease (STD) are caused adopts antibiotic therapy mostly, and the therapeutic effect of antibiotics is obvious, and speed is fast, but not remarkable to the chronic disease therapeutic effect, the medication that has also can produce the drug-resistant effect to antibacterial too for a long time.A kind of compound recipe Herba Lysimachiae preparation is disclosed in the prior art (CN200710123150.1), but because shortcoming and restriction that its prescription Chinese medicine raw material is selected for use, so therapeutic effect is undesirable, therefore, the present invention is according to Chinese medical theory, through experiment screening for many years, again the material medicine in the prescription is selected, optimized preparation technology, improved the relieving stranguria by diuresis of medicine greatly, the clearing heat for detumescence effect, having beyond thought significant curative effect aspect the treatment diseases of urinary system, especially produced prior art and write out a prescription not available antibiotic, resistance prostatitis, analgesia, the windfall effect of function of gallbladder promoting.
Summary of the invention
The object of the present invention is to provide the Chinese medicine preparation of a kind of antiinflammatory, pain relieving, antibiotic, calculus, function of gallbladder promoting.
Another object of the present invention is to provide a kind of preparation method of this Chinese medicine preparation.
A further object of the invention is to provide the application of this Chinese medicine preparation in the medicine of preparation treatment urinary system calculus and urinary tract infection.
In addition, the present invention also provides the application of this Chinese medicine preparation in the medicine of preparation treatment renal calculus, prostatitis, cholelithiasis and cholecystitis.
The active ingredient of Chinese medicine preparation provided by the invention is prepared from by the following weight Chinese medicinal raw materials:
Herba Desmodii Styracifolii 200-220 part Herba Plantaginis 100-115 part Herba Pyrrosiae Calvatae 100-115 part Stigma Maydis 45-60 part
The active ingredient of Chinese medicine preparation of the present invention preferably is prepared from by the following weight proportion raw material:
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
The active ingredient of Chinese medicine preparation of the present invention can also preferably be prepared from by the following weight proportion raw material:
60 parts of 100 parts of Stigma Maydis of 115 parts of Herba Pyrrosiae Calvataes of 220 portions of Herba Plantaginiss of Herba Desmodii Styracifolii.
Chinese medicine preparation of the present invention can be prepared into various preparations by the ordinary skill in the art.
The present invention also provides the preparation method of this Chinese medicine preparation, and this method comprises the steps:
Get above-mentioned four flavor Chinese medicines, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 0.5-2.5 hour for the first time, 0.5-1.5 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, make various preparations by this area routine techniques.Wherein preferably use the ethanol of 70-95%.
1, the production method of granule: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add sucrose, make granule, be drying to obtain; Or get above compound, and concentrate, add lactose, dextrin and sweeting agent, make granule, be drying to obtain.
2, the production method of tablet: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient and granulate, drying adds disintegrating agent, lubricant, tabletting, or directly pack, or sugar coating, or the bag film-coat promptly obtains product.
The described excipient of above tablet is starch and derivant, dextrin, carboxymethyl starch, calcium hydrogen phosphate and other pharmaceutic adjuvants; Described disintegrating agent is sodium carboxymethyl cellulose, hypromellose and pharmaceutic adjuvant with water swelling; Described lubricant is the pharmaceutic adjuvant of Polyethylene Glycol, magnesium stearate, Pulvis Talci and tool lubrication; Described sugar coating material is sucrose, Pulvis Talci, gelatin, pigment, river wax etc.; Described thin film coating material is the water of stomach dissolution type, pure molten coating material.Wherein excipient, disintegrating agent, lubricant, sugar coating, the weight content of bag film-coat material in medicine are respectively: 5～70%, 1～30%, 0.1～10%, 35～90%, 1.5～5%.
3, the production method of capsule: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient, disintegrating agent mixing, dry, pulverizing, add fluidizer; Or make particle drying, add lubricant; Or make micropill; In incapsulating at last, obtain product.
More than the described excipient of preparation capsule is starch, dextrin, carboxymethyl starch, calcium hydrogen phosphate and other pharmaceutic adjuvants; Described disintegrating agent is sodium carboxymethyl cellulose, hydroxypropyl cellulose and pharmaceutic adjuvant with water swelling; Described fluidizer (lubricant) is that magnesium stearate, differential silica gel and other have the pharmaceutic adjuvant of fluidizer effect.Excipient, disintegrating agent, the weight content of fluidizer (lubricant) in medicine are:: 5～70%, 1～30%, 0.1～10%.
4, the production method of pill: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned concentrated solution that obtains and mixed with excipients, make concentrated pill; Or add Mel again, make water-honeyed pill, promptly.Excipient that adds and the content in medicine thereof are starch and derivant 30～90% thereof, disintegrating agent 3～50%, Mel 10～50%.
5, the production method of mixture: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains and correctives, antiseptic solution mix homogeneously, promptly.Correctives that adds and the content in medicine thereof: sucrose (or Mel) 5～20%, Pyrusussuriensis acids antiseptic such as potassium sorbate 0.1～0.3%, paraben preservative such as ethyl hydroxybenzoate 0.01～0.05%.
6, the production method of syrup: get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 70-95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains and sucrose, antiseptic solution mix homogeneously, promptly.Correctives that adds and the content in medicine thereof: sucrose 45～70%, Pyrusussuriensis acids antiseptic such as potassium sorbate 0.1～0.3%, paraben preservative such as ethyl hydroxybenzoate 0.01～0.05%.
Urinary tract infection is clinical common disease, and the medicine of treatment urinary tract infection has multiple, and is many in the past with the treatment of antimicrobial drugs such as sulfonamides, aminoglycosides, cephalosporins.Because being extensive use of of these medicines, its Resistant strain increases gradually, and is not satisfactory sometimes to the urinary tract infection curative effect.Chinese medicine preparation Main Ingredients and Appearance of the present invention is Herba Desmodii Styracifolii, Herba Plantaginis, Herba Pyrrosiae Calvatae, Stigma Maydis, main liver, lung, kidney, bladder four warps of returning.Chinese medicine preparation of the present invention has anti-inflammation, calculus, analgesia, the lithogenous effect of prevention, both can stop calculus to form, but calculus again, have simultaneously analgesic activity again, for damp-heat in the urinary bladder, urinary tract infection, lithangiuria, renal colic etc. good auxiliary treatment effect is arranged, and almost non-toxic property.Clinical in lithangiuria, renal colic, urinary tract infection, frequent micturition, urgent micturition, dark coloured urine, dysurea have calculus effect preferably to established lithangiuria; Can be as external supersonic ripple rubble adjuvant drug, simultaneously, frequent micturition, urgent micturition that urinary tract infection is caused have curative effect preferably, and comparing with the product of prior art has in addition also increased antibiotic, resistance prostatitis, analgesic windfall effect.
Herba Desmodii Styracifolii is the aerial parts of leguminous plant Desmodium styracifolium (Osb.) Merr., is distributed in provinces and regions such as Guangxi, Guangdong, Fujian and Hunan.Its branch and leaf can be used as medicine, diseases such as treatment urinary tract infection, urinary system calculus, cholelithiasis.Modern study proves that this plant chemical ingredient studies carefully, and separating has chemical compounds such as Xia Fota glycosides, desmodimine, desmodilactone, triterpenoid, lupin ketone and lupeol, tricosane, stearic acid, arachidic acid, Semen arachidis hypogaeae alcohol ester and cupreol.Notice that differentiation has multiple Herba Lysimachiae, the leguminous plant Herba Desmodii Styracifolii has diuresis, calculus, antiinflammation, for commonly using in Guangxi, Guangdong Province; The labiate Herba Glechomae is controlled urinary tract infection, falls damage, and the jaundice due to damp-heat effect is for commonly using in Zhejiang, Jiangsu; Also have and do that Herba Lysimachiae uses Flos Inulae section plant Herba Dichodrae (having another name called the lobule Herba Lysimachiae) arranged, clearing away heat and promoting diuresis, anti-inflammatory analgetic effect are arranged.
Herba Plantaginis has another name called Herba Plantaginis, wheel dish, donkey ear dish, cash strings grass etc., is the dry herb of Plantaginaceae plant Herba Plantaginis (Plantago asiatical Willd) or Plantago depressa Willd (Plantago depressa Willd).Herba Plantaginis, sweet in the mouth, cold in nature is gone into liver, kidney, lung, small intestine meridian, can inducing diuresis for treating stranguria syndrome, eliminating dampness by diuresis antidiarrheal, liver heat removing tomorrow, again can the relieving cough and reducing sputum, removing pathogenic heat from blood and toxic substance from the body.The Chinese medicine document is introduced Herba Plantaginis can treat urinary tract infection.The document record that has is that principal agent can also be treated pneumonia, hematuria, gastric flu and child disease with the Herba Plantaginis.Chemical analysis results, main effective ingredient is ursolic acid, plantagin, aucubin etc. in the Herba Plantaginis.Modern pharmacological research shows, ursolic acid has stronger anti-tumor activity, it not only has resistant function to multiple carcinogenic, short cancer thing, and to multiple malignant cell for example P388 and L4210, leukaemia, A-549 human lung adenocarcinoma cell the growth of inhibition effect is arranged, it also has mutation, antioxidation and inducing cancer cell differentiation and strengthens the effect of aspects such as cellular immune function simultaneously.
Herba Pyrrosiae Calvatae is the Polypodiaceae plant, has relieving stranguria by diuresis, the effect of clearing away heat to stop bleeding, and clinical pyretic stranguria, stranguria with blood, the stranguria caused by urinary stone of being used for the treatment of, urinary obstruction is drenched the puckery pain of drop and is spitted blood epistaxis, hematuria, metrorrhagia, dyspnea and cough due to lung-heat.Compare with the Folium Pyrrosiae of prior art, its active constituents of medicine exists significantly different, the active component of Folium Pyrrosiae mainly is a flavonoid, saponin, the anthraquinone class, tannin and cupreol material, and the active component of Herba Pyrrosiae Calvatae mainly is a sucrose, chimonin, caffeic acid, cupreol, Isomangiferin and Fumaric acid, especially the content of chimonin is apparently higher than Folium Pyrrosiae, because Herba Pyrrosiae Calvatae and Folium Pyrrosiae are in active constituents of medicine and some differences in nature, a lot of beyond thought curative effects have been produced after causing the present invention to use Herba Pyrrosiae Calvatae to substitute Folium Pyrrosiae, the new prescription of forming significantly improves than the therapeutic effect of prior art prescription, has also produced antibiotic simultaneously, resistance prostatitis, the analgesic windfall effect.
Stigma Maydis is zeistic style of grass and stigma.Semen Maydis has another name called corn, luxuriant reed, maize etc., and it is China's important crops, and all there is cultivation all parts of the country, and output is very high.Stigma Maydis is a kind of Chinese medicine, and its nature and flavor are sweet, flat, nontoxic, diuresis is arranged, expels the heat-evil, the effect of suppressing the hyperactive liver, function of gallbladder promoting, and it not only can be used as the single medicinal material decoction, also can use with other drug matching, is used for treating nephritis, cholelithiasis, diabetes etc.In recent years, pharmacological research shows that Stigma Maydis extract also has blood pressure lowering and antitumaous effect.Because most Stigma Maydis are dropped, to fail to make full use of, the relevant also rare report of Stigma Maydis The Chemical Constituents has bibliographical information employing gas chromatography-mass spectrum-computer coupling separation and has identified the chemical constituent of volatile oil in the Stigma Maydis.The result shows, 42 compositions from 57 chromatographic peaks, have been identified, wherein main chemical constituent has heneicosane (6.53%), nonacosane (10.70%), hexatriacontane (4.11%), Ethyl linoleate (3.57%), bean steroid 5-alkene-3-alcohol (6.67%), cupreol (7.89%); Stigmasterol (6.82%).
Beneficial effect
1, pharmacology, drug efficacy study
A kind of compound recipe Herba Lysimachiae preparation is disclosed in the prior art (CN200710123150.1), but because shortcoming and restriction that its prescription Chinese medicine raw material is selected for use, so therapeutic effect is undesirable, therefore, the present invention is according to Chinese medical theory, through experiment screening for many years, again the material medicine in the prescription is selected, optimized preparation technology, thereby make drug prescription more reasonable, active constituents of medicine obtains more rational adjustment, improved bioavailability greatly, improved the relieving stranguria by diuresis of medicine greatly, the clearing heat for detumescence effect having beyond thought significant curative effect aspect the treatment diseases of urinary system, has especially produced prior art and has write out a prescription not available antibiotic, resistance prostatitis, the analgesic windfall effect.
Contrast shows that the drug effect of medicine of the present invention obviously is better than prior art, and is specific as follows through pharmacodynamic experiment:
One, material
Test drug: the compound recipe Herba Lysimachiae granule of this test adopts the technical scheme (hereinafter to be referred as " Herba Pyrrosiae Calvatae side ") of the embodiment of the invention 3, is supplied with lot number: 090211 by Wantong Pharmaceutical Co., Ltd., Guangxi.
The compound recipe Herba Lysimachiae granule of prior art (CN200710123150.1) adopts the technical scheme (hereinafter to be referred as " Folium Pyrrosiae side ") of embodiment 3, is supplied with lot number 090210 by Wantong Pharmaceutical Co., Ltd., Guangxi:.
Animal: Kunming mouse, the Wistar rat provides by Guangxi Medical University's Experimental Animal Center, the animal quality certification number: SCXK osmanthus 2003-2003.
Bacterial strain: provide by clinical laboratory of No.1 Hospital Attached to Guangxi Medical Univ..
Instrument: polarizing microscope, optical instrument factory, Shanghai; GJ-8402 hot plate dolorimeter, the white stone electronic medical instruments in middle sea factory; Ware electronic balance on the JA1003 type, balance factory of last Nereid section; The double single face clean work station of SW-CJ-2FD, Purifying Equipment Co., Ltd., Suzhou makes; The SKP.01 electro-heating standing-temperature cultivator, Huangshi city medical apparatus and instruments factory.
Two, method and result:
＜one 〉, to the lithogenous effect of kidney of rats:
Get 70 of normal health rats, male and female half and half, body weight 250～300g.Be divided into 7 groups at random, 10 every group, i.e. normal control group, model group, the total medical material/kg of Folium Pyrrosiae side group 15.0g, Herba Pyrrosiae Calvatae side's group 15.0,7.5,3.25 total medical material g/kg3 dosage groups.Except that the normal control group, other are respectively organized rat and irritate stomach every day and lure stone agent (10g/L ethylene glycol+20g/L aqueous ammonium chloride solution) 2mL.In modeling, each administration group gastric infusion volume is 0.2ml/100g, and normal control group and model group give equal-volume normal saline every day 1 time, 4w continuously.After experiment finishes, put to death rat, get the nephridial tissue conventional organization and cut back E dyeing, polarized light microscope observing kidney pathological change and calcium oxalate crystal situation, take statistics by 0～5 grade of criterion, (1) no crystallization, the crystallization of (+) renal medulla, the crystallization of (++) renal cortex, (+++) the renal papillae crystallization, (++ ++) the renal calices intercrystalline, (++ +++) the ureter intercrystalline, the results are shown in Table 1.
The comparison of the effect of table 1 pair large mouse with renal calculs
Annotate: and model group is relatively, * p＜0.05, * * p＜0.01 and Folium Pyrrosiae side's group compare, △ p＜0.05
The result shows that the total medical material of Herba Pyrrosiae Calvatae side 15g/kg dosage group obviously alleviates the pathological state of large mouse with renal calculs kidney; Compare with model group, the total medical material of Herba Pyrrosiae Calvatae side 15g/kg dosage group can significantly reduce the content of large mouse with renal calculs kidney oxalic acid and calcium, and more remarkable than Folium Pyrrosiae side's effect; 7.5g total medical material/kg dosage group can significantly reduce the content of large mouse with renal calculs kidney calcium; The total medical material of Herba Pyrrosiae Calvatae side 3.25g/kg dosage group does not make significant difference to the content of large mouse with renal calculs kidney oxalic acid and calcium.Prompting Herba Pyrrosiae Calvatae side compares renal calculus with Folium Pyrrosiae side and has significant therapeutical effect.
＜two 〉, diuresis:
Get 60 of normal health rats, male and female half and half, body weight 250-300g.Be divided into 6 groups at random, 10 every group, i.e. normal control group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, the total medical material/kg3 of a 3.25g dosage group, the gastric infusion volume is 0.2mL/10g, and the normal control group gives the equal-volume normal saline.Every day 1 time, continuous 6d, 7d reference literature method, each Mus is pressed the normal saline lumbar injection of 2mL/100g, and gently depress abdominal part and make the bladder emptying, simple and easy metabolic cage is put into rat in administration then immediately behind the medicine, collect the urine (per hour counting the urine amount 1 time) of discharging in the 5d after each Mus administration, the results are shown in Table 2
The comparison of the influence of table 2 pair normal saline load rat urine amount (x ± S, n=10)
Annotate: and the normal control group is relatively, * p＜0.05, * * p＜0.01
The result shows, the total medical material of Herba Pyrrosiae Calvatae side 15g/kg dosage group after administration 2,3,4,5h can obviously increase normal saline load rat urine amount, 7.5g total medical material/kg dosage group after administration 3,4,5h can obviously increase normal saline load rat urine amount, the total medical material of 3.25g/kg dosage group 5h after administration can obviously increase normal saline load rat urine amount.Prompting Herba Pyrrosiae Calvatae side compares with Folium Pyrrosiae side has significant diuresis.
＜three 〉, external bacteriostasis:
The pathogenic strain of clinical separation and evaluation is cultivated 18h in nutrient broth, regulate bacterial concentration with sterile saline before each experiment.The concentration of measuring the bacterium liquid that tries than turbid instrument with bacterium colony be 0.5 Maxwell unit (108/mL).Adopt agar doubling dilution test preparation medicine, form the pastille MH culture medium of finite concentration gradient, with 10 μ L inoculating loops with the dibbling of bacterium liquid in the pastille plate, and establish a negative contrast of plate that does not contain medicine.Bacterium liquid final concentration is every 106/mL, and in 35% cultivation 24d, observed result does not see that the lowest concentration of drug of bacterial growth is the MIC of this medicine, the results are shown in Table 3.
The comparison of table 3 vitro antibacterial activity
The result shows, Herba Pyrrosiae Calvatae side all has in various degree bacteriostasis to Pseudomonas aeruginosa, Escherichia coli, klebsiella pneumoniae, Acinetobacter baumannii, staphylococcus aureus, staphylococcus epidermidis etc., and Folium Pyrrosiae side is not obvious to above-mentioned bacterial action.Prompting Herba Pyrrosiae Calvatae side compares bacterium such as Pseudomonas aeruginosa, Escherichia coli, klebsiella pneumoniae, Acinetobacter baumannii, staphylococcus aureus, staphylococcus epidermidis with Folium Pyrrosiae side and has unexpected significant bacteriostasis.
＜four 〉, choleretic effect:
Get 60 of normal health rats, male and female half and half, body weight 250-300g.Be divided into 6 groups at random, every group 10, it is the normal control group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, be duodenal administration, the administration volume is 0.2mL/10g, and normal control group and model group give the equal-volume normal saline.Fasting 12～16h before the experiment, urethane (1g/kg) anesthesia, back of the body position is fixing, find stomachus pyloricus at upper right abdomen after opening abdomen, with the pylorus is standard, and the upset duodenum can be seen milky vater's papilla portion, track common bile duct from pars papillaris, the tunicle that will cover the surface with pincet is peeled off several millimeters to expose common bile duct.Inserting external diameter to the nearly liver of common bile duct end is plastic tube about 1mm, with bile drainage.After treating that bile is stable, and the beginning administration.After the record administration 1,2,3h bile output, the results are shown in Table 4.
The comparison of the influence of table 4 pair rat bile flow (x ± S, n=10)
Annotate: and the normal control group is relatively, * p＜0.05, * * p＜0.01
The result shows, 2h after the administration, and the total medical material of Herba Pyrrosiae Calvatae side 15g/kg dosage group can significantly improve the rat bile flow, and Folium Pyrrosiae side is to this no effect.Prompting Herba Pyrrosiae Calvatae side compares with Folium Pyrrosiae side has significant more choleretic effect.
＜five 〉, resistance prostatitis:
Get 70 of normal health rats, male and female half and half, body weight 250-300g.Be divided into 7 groups at random, every group 10, it is the normal control group, model group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, the gastric infusion volume is 0.2ml/10g, and normal control group and model group give the equal-volume normal saline.1h after the 9d administration opens rat abdominal cavity under the pentobarbital anesthesia, and except that normal group, each is organized and injects 1% aseptic carrageenin normal saline solution 0.1mL in the rat prostate siphonal lobe, and normal rats is injected physiological saline solution solution 0.1mL, sews up.Behind the last administration 24h, with rat anesthesia, get massage of prostate liquid, microscopy is leukocyte count wherein, and gets a prostatic fluid, the side of pushing away checks lecithin density, by the clinical criteria classification: the full visual field is 4 grades, and 3/4 visual field is 3 grades, and 1/2 visual field is 2 grades, 1/4 visual field is 1 grade, carries out statistical procedures.Other gets part rat prostate tissue, with formalin fixed, carries out histological examination, the results are shown in Table 5.
The comparison of the influence of table 5 pair rat experiment prostatitis model (x ± S, n=10)
Annotate: and model group is relatively, * p＜0.05, * * p＜0.01
The result shows that the total medical material of Herba Pyrrosiae Calvatae side 15g/kg dosage group can significantly improve prostatitis rat lecithin, reduces numeration of leukocyte, and the total medical material of 15g/kg dosage group can show the reduction numeration of leukocyte, and Folium Pyrrosiae side does not have remarkable effect.Prompting Herba Pyrrosiae Calvatae side has the prostatitic windfall effect of the not available treatment in Folium Pyrrosiae side.
＜six 〉, antiinflammatory action
1, the bullate influence of rat foot due to the on Carrageenan
Get 60 of normal health rats, male and female half and half, body weight 250-300g.Be divided into 6 groups at random, 10 every group, i.e. model group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, the gastric infusion volume is 0.2mL/10g, and model group gives the equal-volume normal saline.Continuous gastric infusion 7d, 1h after the last administration, with the left back sufficient plantar subcutaneous injection of every 0.1mL of 1% carrageenin physiological salt liquid, cause scorching before and cause scorching back 1,2,4h measures sufficient sole of the foot thickness with micrometer callipers, cause scorching forward and backward sufficient sole of the foot thickness difference as the swollen index of foot, the results are shown in Table 6.
The comparison of the bullate influence of rat foot due to table 6 on Carrageenan (x ± S, n=10)
Annotate: compare * p＜0.05, * * p＜0.01 with matched group
The result shows, causes scorching back 1,2h, and the rat foot is bullate due to Herba Pyrrosiae Calvatae side 15.0, the 7.5g crude drug/kg dosage group on Carrageenan an obvious suppression effect.Prompting Herba Pyrrosiae Calvatae side compares with Folium Pyrrosiae side has antiinflammatory action.
2, to the influence of rat granuloma hamartoplasia
Get 60 of normal health rats, male and female half and half, body weight 250-300g.Be divided into 6 groups at random, 10 every group, i.e. model group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, the gastric infusion volume is 0.2ml/10g, and model group gives the equal-volume normal saline.Lumbar injection pentobarbital sodium 30mg/kg anesthesia, cut off big groin Mus hair, use iodine disinfection, after 75% alcohol swab takes off iodine, cut-the long otch of 1.0cm, implant subcutaneous from incision the autoclaving cotton balls of 20mg with the ophthalmology tweezers, skin suture immediately, from performing the operation the same day continuous gastric infusion 7d, 24h after the last administration, open otch, cotton balls is taken out together with connective tissue on every side, reject fatty tissue, in 70 ℃ of baking ovens of constant temperature behind the dry 48h, the applied analysis balance is weighed, with claim weight deduct cotton balls weight (20mg), promptly get granuloma weight, result of calculation sees Table 7.
The comparison of the influence of table 7 pair rat granuloma hamartoplasia (x ± S, n=10)
Annotate: compare * p＜0.05, * * p＜0.01 with model control group
The result shows, Herba Pyrrosiae Calvatae side group 7.5g crude drug/kg has an obvious suppression effect to rat granuloma hamartoplasia.Prompting Herba Pyrrosiae Calvatae side compares with Folium Pyrrosiae side has remarkable antiinflammatory action.
＜seven 〉, analgesic activity
1, Dichlorodiphenyl Acetate causes that the influence of mouse writhing reaction gets 60 of Kunming kind white mice, male and female half and half, body weight 18～22g, 10 every group.Be divided into 7 groups at random, it is model group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, the gastric infusion volume is 0.2mL/10g, model group gives the equal-volume normal saline, continuous gastric infusion 7d, 1h after the last administration, the equal lumbar injection 0.6% acetum 0.2mL/ of each Mus are only, the writhing response number of times that each Mus occurs in the counting 10min behind the 5min, result such as table 8.
Table 8 Dichlorodiphenyl Acetate induced mice turn round the influence of body experiment comparison (x ± S, n=10)
Annotate: compare * p＜0.05, * * p＜0.05 with matched group
The result shows that Herba Pyrrosiae Calvatae side group 15.0g crude drug/kg dosage group mouse writhing number of times is significantly less than matched group.Prompting Herba Pyrrosiae Calvatae side has remarkable analgesic activity with respect to Folium Pyrrosiae side.
(putting into hot plate to the metapedes required time occurring licking from mice) is 60 of the mices of 5～20s when 2, the influence of pain threshold due to the mice hot plate being reacted bitterly with GJ-8402 hot plate dolorimeter (55 ± 5) ℃ screening hot plate, be divided into 6 groups at random, every group each 10, it is model group, the total medical material of Folium Pyrrosiae side group 15.0g/kg dosage group, Herba Pyrrosiae Calvatae side's group 15.0,7.5, three dosage groups of the total medical material/kg of 3.25g, the gastric infusion volume is 0.2ml/10g, and model group gives the equal-volume normal saline.When reacting bitterly with twice average hot plate as the basic threshold of pain.Behind each medicine of mouse stomach administration 30,60,120,180min measures the threshold of pain, then takes out by 60s result such as table 9 as surpassing the 60s nonresponder.
The comparison of the influence of table 9 pair hot plate pain time (x ± S, n=10)
Annotate: compare * p＜0.05, * * p＜0.05 with model group
The result shows, Herba Pyrrosiae Calvatae side 15.0g crude drug/kg dosage group after administration 60,120min all can significantly reduce mice hot plate pain threshold.Prompting Herba Pyrrosiae Calvatae side has remarkable analgesic activity with respect to Folium Pyrrosiae side.
2, toxicity test
Choose 40 of healthy adult mices, observe 6d, be divided into 2 groups then at random, be i.e. normal control group and Herba Pyrrosiae Calvatae side's group, 20 every group, male and female half and half.Irritate stomach and give Cmax (185g/kg), maximum volume (40ml/kg body weight) Herba Pyrrosiae Calvatae side's hydrotrope, observe 14d then.Observe diet situation, body weight, signs of toxicity (comprising outward appearance behavior, secretions, excretion thing etc.) and the death condition of mice, put to death mice behind the 14d, dissect the state that mice is observed major organs.
The result shows, 30min behind the filling stomach, and normal control group and Herba Pyrrosiae Calvatae Fang Zujun do not have uncomfortable symptom.In the 14d, all mices all do not have dead the generation, and diet, outward appearance, behavior, secretions, excretion thing are all no abnormal, the body weight normal growth.Behind the 14d, put to death mice, the situation of anatomic observation main organs compares with the normal control group, and main organs volume, color, quality such as Herba Pyrrosiae Calvatae side's group mouse core, liver, lung are all no abnormal.The toxicity of prompting Herba Pyrrosiae Calvatae side is low.
3, sum up
Above experimental result shows, the Herba Pyrrosiae Calvatae side of new prescription compares with Folium Pyrrosiae side of the prior art has outstanding substantive distinguishing features and obvious improvement, can significantly alleviate the pathological state of large mouse with renal calculs kidney, significantly reduce the content of large mouse with renal calculs kidney oxalic acid and calcium; Significantly improve normal saline load rat urine amount; Pseudomonas aeruginosa, Escherichia coli, klebsiella pneumoniae, Acinetobacter baumannii, staphylococcus aureus, staphylococcus epidermidis etc. had bacteriostasis; Significantly improve the rat bile flow; Significantly improve prostatitis rat lecithin, reduce numeration of leukocyte; Significantly the rat foot is swollen due to the inhibition carrageenin, suppresses rat granuloma hamartoplasia; Significantly suppress the mouse writhing reaction that acetic acid causes, significantly reduce mice hot plate pain threshold.Herba Pyrrosiae Calvatae side of the present invention has significant eliminating nephrolith, diuresis, function of gallbladder promoting, antibiotic, resistance prostatitis, antiinflammatory, analgesic effect.Wherein the effect of the eliminating nephrolith of Herba Pyrrosiae Calvatae side is significantly more a lot of than Folium Pyrrosiae side, antibiotic, resistance prostatitis, analgesia, function of gallbladder promoting act as the indication that Herba Pyrrosiae Calvatae side increases newly.
The specific embodiment
Further specify technical scheme of the present invention below by specific embodiment.
Embodiment 1
50 parts of 98 parts of Stigma Maydis of 100 parts of Herba Pyrrosiae Calvataes of 200 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.16～1.22 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.16 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 70% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add sucrose, make granule, be drying to obtain;
Embodiment 2
60 parts of 100 parts of Stigma Maydis of 115 parts of Herba Pyrrosiae Calvataes of 220 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 85% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, get above compound, concentrate, add lactose and sweeting agent, make granule, be drying to obtain.
Embodiment 3
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.16～1.22 the cream that disappears that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, it is 1.10～1.16 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, left standstill 24 hours, and got supernatant, reclaim ethanol, concentrate; With above-mentioned clear paste mixing, add lactose, dextrin and sweeting agent, make granule, be drying to obtain.
Embodiment 4
54.45 parts of 108.9 parts of Stigma Maydis of 108.9 parts of Herba Pyrrosiae Calvataes of 217.8 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, collecting decoction filters, and it is 1.15～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and 1.5 hours for the first time, 1 hour for the second time, collecting decoction filtered, it is 1.10～1.15 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, left standstill 24 hours, and got supernatant, reclaim ethanol, concentrate; With above-mentioned clear paste mixing, get above compound, concentrate, add lactose, dextrin and sweeting agent, make granule, be drying to obtain.
Embodiment 5
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient and granulate, drying adds disintegrating agent, lubricant, tabletting, coating promptly obtains product.
Embodiment 6
54.5 parts of 100 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient, disintegrating agent mixing, dry, pulverizing; Or make particle drying, add fluidizer; Or make micropill; In incapsulating then, obtain product.
Embodiment 7
60 parts of 115 parts of Stigma Maydis of 115 parts of Herba Pyrrosiae Calvataes of 220 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.15～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.20 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add sucrose, make granule, be drying to obtain; Or get above compound, and concentrate, add lactose and sweeting agent, make granule, be drying to obtain.
Embodiment 8
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.16～1.22 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, it is 1.10～1.16 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, left standstill 24 hours, and got supernatant, reclaim ethanol, concentrate; With above-mentioned clear paste mixing, add sucrose, make granule, drying, promptly.
Embodiment 9
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.16～1.22 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.16 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With adding excipient in the above-mentioned concentrated solution that obtains, make concentrated pill or water-honeyed pill promptly.
Embodiment 10
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains, add sucrose (or Mel) 5～20%, potassium sorbate 0.1～0.3%, ethyl hydroxybenzoate 0.01～0.05%, promptly.
Embodiment 11
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, collecting decoction filters, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains, add sucrose 45～70%, potassium sorbate 0.1～0.3%, ethyl hydroxybenzoate 0.01～0.05%.
Embodiment 12
54.45 parts of 108.9 parts of Stigma Maydis of 108.9 parts of Herba Pyrrosiae Calvataes of 217.8 portions of Herba Plantaginiss of Herba Desmodii Styracifolii
Get above-mentioned four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, collecting decoction filters, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount 95% ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains, add sucrose 65%, potassium sorbate 0.2%, ethyl hydroxybenzoate 0.03%.
Claims (18)
1. the Chinese medicine preparation of an anti-inflammatory analgetic, antibiotic calculus, function of gallbladder promoting, it is characterized in that: its active ingredient is prepared from by following raw materials by weight proportions:
Herba Desmodii Styracifolii 200-220 part Herba Plantaginis 100-115 part Herba Pyrrosiae Calvatae 100-115 part Stigma Maydis 45-60 part.
2. Chinese medicine preparation as claimed in claim 1 is characterized in that: the weight proportion of described crude drug is:
54.5 parts of 109 parts of Stigma Maydis of 109 parts of Herba Pyrrosiae Calvataes of 218 portions of Herba Plantaginiss of Herba Desmodii Styracifolii.
3. Chinese medicine preparation as claimed in claim 1 is characterized in that: the weight proportion of described crude drug is:
60 parts of 100 parts of Stigma Maydis of 115 parts of Herba Pyrrosiae Calvataes of 220 portions of Herba Plantaginiss of Herba Desmodii Styracifolii.
4. as any described Chinese medicine preparation of claim 1-3, it is characterized in that: it is granule, tablet, capsule, pill, mixture, syrup.
5. as the preparation method of any described Chinese medicine preparation of claim 1-4, it is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 0.5-2.5 hour for the first time, 0.5-1.5 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, make various preparations by this area routine techniques.
6. as the preparation method of any described Chinese medicine preparation of claim 1-4, it is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 0.5-2.5 hour for the first time, 0.5-1.5 hour for the second time, collecting decoction filtered, it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～3 times of amount ethanol, stir evenly, left standstill 24 hours, and got supernatant, reclaim ethanol, concentrate, get concentrated solution; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities; With above-mentioned concentrated solution mixing, make various preparations by this area routine techniques.
7. the preparation method of Chinese medicine preparation as claimed in claim 5, it is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.16～1.22 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.16 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1.5 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add adjuvant, make granule, be drying to obtain granule.
8. the preparation method of Chinese medicine preparation as claimed in claim 5, it is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient, to granulate, drying adds disintegrating agent, lubricant, tabletting, or directly pack, or sugar coating, or the bag film-coat promptly obtains tablet.
9. the preparation method of Chinese medicine preparation as claimed in claim 8, it is characterized in that: the excipient described in this method is starch and derivant, dextrin, carboxymethyl starch, microcrystalline Cellulose or calcium hydrogen phosphate; Described disintegrating agent is that sodium carboxymethyl cellulose, hypromellose or other have the pharmaceutic adjuvant of water swelling; Described lubricant is the pharmaceutic adjuvant of Polyethylene Glycol, magnesium stearate, Pulvis Talci or other tool lubrication; Described sugar coating material is sucrose, Pulvis Talci, gelatin, pigment and river wax; Described thin film coating material is stomach dissolution type water or pure molten coating material.
10. the preparation method of Chinese medicine preparation as claimed in claim 5, it is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.38 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.08～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned clear paste mixing, add excipient, disintegrating agent mixing, dry, pulverizing, add fluidizer: make particle drying, add lubricant; Or make micropill; In incapsulating at last, obtain capsule.
11. the preparation method of Chinese medicine preparation as claimed in claim 10 is characterized in that: the excipient described in this method is starch, dextrin, carboxymethyl starch or calcium hydrogen phosphate; Described disintegrating agent is that sodium carboxymethyl cellulose, hydroxypropyl cellulose or other have the pharmaceutic adjuvant of water swelling; Described fluidizer is that magnesium stearate, differential silica gel or other have the pharmaceutic adjuvant of fluidizer effect.
12. the preparation method of Chinese medicine preparation as claimed in claim 5 is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With above-mentioned concentrated solution that obtains and mixed with excipients, make concentrated pill or water-honeyed pill, promptly get pill.
13. the preparation method of Chinese medicine preparation as claimed in claim 5 is characterized in that: this method comprises the steps:
Get described four Chinese medicine thing, Herba Desmodii Styracifolii, Herba Plantaginis, Stigma Maydis three flavors are decocted with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, and it is 1.10～1.30 clear paste that filtrate is concentrated into 70 ℃ of relative densities; Herba Pyrrosiae Calvatae decocts with water secondary, and collecting decoction filters, and it is 1.10～1.25 clear paste that filtrate is concentrated into 70 ℃ of relative densities, puts coldly, adds 1～2 times of amount ethanol, stirs evenly, and leaves standstill 24 hours, gets supernatant, reclaims ethanol, and is concentrated; With the above-mentioned concentrated solution that obtains and correctives, antiseptic solution mix homogeneously, promptly get mixture or syrup.
14. as the application of any described Chinese medicine preparation of claim 1-3 in the medicine of preparation treatment urinary system calculus.
15. as the application of any described Chinese medicine preparation of claim 1-3 in the medicine of preparation treatment urinary tract infection.
16. as the application of any described Chinese medicine preparation of claim 1-3 in the medicine of preparation treatment renal calculus.
17. as the application of any described Chinese medicine preparation of claim 1-3 in the prostatitic medicine of preparation treatment.
18. as the application of any described Chinese medicine preparation of claim 1-3 in the medicine of preparation treatment cholecystitis.
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|---|---|---|---|
| CN2009101145308A CN101698066B (en) | 2009-11-05 | 2009-11-05 | Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof |
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| CN2009101145308A CN101698066B (en) | 2009-11-05 | 2009-11-05 | Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof |
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| CN101698066A CN101698066A (en) | 2010-04-28 |
| CN101698066B true CN101698066B (en) | 2010-09-29 |
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| CN2009101145308A Active CN101698066B (en) | 2009-11-05 | 2009-11-05 | Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102357215B (en) * | 2011-11-16 | 2013-01-02 | 河南中医学院 | Ointment for treating right hypochondriac distention and pain caused by acute and chronic cholecystitis |
| CN102579841A (en) * | 2012-03-26 | 2012-07-18 | 周洁 | Health-care herbal medicine for treating prostatitis |
| CN103495064B (en) * | 2013-10-14 | 2018-06-15 | 广西万通制药有限公司 | A kind of application of Chinese medicine preparation in prevention and treatment chronic renal failure and kidney fibrosis is prepared |
| CN103520535B (en) * | 2013-10-14 | 2018-06-15 | 广西万通制药有限公司 | Application of the compound desmodium preparation in prevention and treatment chronic renal failure and kidney fibrosis is prepared |
| CN104013681B (en) * | 2014-07-01 | 2016-05-11 | 广西浦北华之源中药饮片有限公司 | A kind of pharmaceutical composition for the treatment of kidney stone |
| CN104721407A (en) * | 2015-04-01 | 2015-06-24 | 宁宁 | Traditional Chinese medicine composition for treating liver-depression and qi-stagnation type cholecystitis |
| CN114099492B (en) * | 2021-12-31 | 2023-03-24 | 山东省妇幼保健院 | Application of natural medicine in preparing antibacterial medicine |
| CN116942767A (en) * | 2023-06-05 | 2023-10-27 | 广西万通制药有限公司 | Medicine application of compound desmodium granules for treating prostatitis |
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