CN101696073A - Two-phase distributing bioreactor and application in processing organic wastewater with high concentration - Google Patents
Two-phase distributing bioreactor and application in processing organic wastewater with high concentration Download PDFInfo
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- CN101696073A CN101696073A CN200910117534A CN200910117534A CN101696073A CN 101696073 A CN101696073 A CN 101696073A CN 200910117534 A CN200910117534 A CN 200910117534A CN 200910117534 A CN200910117534 A CN 200910117534A CN 101696073 A CN101696073 A CN 101696073A
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Abstract
The invention provides a two-phase distributing bioreactor which can effectively improve the tolerance and the extractive phase performance of microorganisms on organic pollutants. The reactor comprises a pool body, wherein the pool body is internally provided with filter screens, and a solid phase extracting agent bed layer and an immobilized microorganism carrier bed layer are alternately arranged among the filter screens from bottom to top; the lower part of the pool body is provided with a water aerating and an air aerating system; the upper part of the pool body is provided with a water outlet pipe; and a solid phase extracting agent of the solid phase extracting agent bed layer is formed in such a way that polysulfone is embedded with nonanol or the polysulfone is embedded with organic imvite. A macroporous carrier immobilized microorganism is introduced into the two-phase distributing bioreactor so that the two-phase distributing bioreactor has more favorable biological tolerance; the nonanol/polysulfone or organic imvite/a polysulfone solid extracting agent is introduced, thereby solving the problems of biodegradation of an organic extractive phase, microorganism growing inhibition, microorganism loss caused by a plurality of foams generated in the aerating process, and the like; and the obtained solid extracting agent has large adsorbing capacity and high adsorbing rate, and the complicated wastewater can be processed by using a mixed bacterium population.
Description
Technical field
The invention belongs to the crossing domain of chemistry, material and environment subject, relate to a kind of two-phase distributing bioreactor; Also relate to the application of two-phase distributing bioreactor in handling high concentrated organic wastewater simultaneously.
Background technology
The two-phase distribution system is usually used in the separation science field, and the two-phase distributing bioreactor (TPPB) that rises the seventies in 20th century is subjected to people's great attention, and the eighties, this technology has obtained widespread use in the extractive fermentation production process.1996, Munro and Daugulis took the lead in carrying out two-phase and have distributed reactor to be used for the Study on degradation of poisonous and harmful organic pollutant.On the traditional sense, two-phase distributing bioreactor is exactly as a carrier that stores and transmit pollutent with hydrophobic organic solvent, the organic pollutant of the high density in the water is extracted, reduced the concentration of poisonous substrate in the water, and then biodegrade.The transfer of substrate in organic solvent and water freely controlled by thermodynamic(al)equilibrium, in the microorganism of aqueous phase the degraded of poisonous substrate destroyed this balance, and in order to keep balance, the poisonous substrate in the organic phase shifts to aqueous phase again.In this manner, just the place one's entire reliance upon metabolic activity of microorganism of the velocity of diffusion of pollutent in two-phase distributing bioreactor, so the degraded of pollutent controlled by the metabolism of self microorganism in the system, and then realized the automatic control of system.The TPPB system is proved to be, and in the improvement of the environment such as water body, atmosphere and soil that toxic organic compound is polluted, two-phase distributing bioreactor has tentatively demonstrated vital role.But caused problems such as microorganism loss because extracted organic phase might produce a large amount of foams in biological degradation, inhibition microorganism growth and the aeration process, brought restriction therefore for the widespread use of this system.For these reasons, single culture and aseptic technique just must be applied in this system.But so not only increased cost, but also can not thoroughly deal with problems, remoter from practical application.Therefore, the critical problem of solution two-phase distributing bioreactor system is exactly to improve microorganism to the tolerance of organic pollutant and the performance of extraction phase.In addition, the extraction agent in traditional two-phase bioreactor is a liquid phase, can not dynamichandling waste water, and also remoter from practical application.
Summary of the invention
The purpose of this invention is to provide a kind of two-phase distributing bioreactor that can effectively improve microorganism to the tolerance performance and the extraction phase performance of organic pollutant.
Another object of the present invention provides the application of two-phase distributing bioreactor in handling high concentrated organic wastewater.
Two-phase distributing bioreactor of the present invention comprises the pond body, is provided with filter screen in the body of pond, and circulation from bottom to top is provided with solid extracting agent bed and fixed microorganism carrier bed between each filter screen; Be provided with expose to the sun water system and aerating system in body bottom, pond; Be provided with rising pipe on body top, pond.Fig. 1 is the structural representation of two-phase distributing bioreactor of the present invention.
The solid extracting agent of described solid extracting agent bed is the solid extracting agent that polysulfones embedding nonyl alcohol or polysulfones embedding organo montmorillonite form.
Wherein polysulfones embedding nonyl alcohol forms the preparation method of solid extracting agent, be under the mechanical stirring condition, polysulfones is dissolved in the N-N-methyl-2-2-pyrrolidone N-with 1: 5～1: 10 bulking value, after the nonyl alcohol that adding polysulfones quality is 3～4 times stirs, splash into the needle tubing that has the 12# syringe needle and form the plate-like bead in the Span-80 aqueous solution of 0.1～0.3wt.%, and after in distilled water, soaking 24h, dry and get final product.
Nonyl alcohol/polysulfones extraction agent Pyrogentisinic Acid's absorption property test: take by weighing 6g nonyl alcohol/polysulfones extraction agent, join initial concentration be respectively 1000,2000,3000,4000 and the phenol solution of 7000mg/L in, under the magnetic agitation condition, regularly measure the concentration of phenol in the solution, result such as Fig. 2.Nonyl alcohol/polysulfones extraction agent has very fast adsorption rate (60 minutes reach adsorption equilibrium) and bigger adsorptive capacity as can be seen from Figure 2.
Polysulfones embedding organo montmorillonite forms the preparation method of solid extracting agent, be under mechanical stirring to be dissolved in N-N-methyl-2-2-pyrrolidone N-with 1: 10～1: 15 weightmeasurement ratio polysulfones, the organo montmorillonite that adds 0.2～1.5 times of polysulfones quality, after stirring under agitation, splash into the needle tubing that has the 12# syringe needle in the ethanol of mass concentration 15～35%, form globe, and globe is soaked the 24h final vacuum be drying to obtain the solid phase extraction agent in distilled water.
Organo montmorillonite/polysulfones extraction agent Pyrogentisinic Acid's absorption property test: take by weighing 6g organo montmorillonite/polysulfones extraction agent, join initial concentration and be 1000,2000,3000 and the phenol solution of 4000mg/L in, under the magnetic agitation condition, regularly measure the concentration of phenol in the solution, result such as Fig. 3.Organo montmorillonite/polysulfones extraction agent has faster adsorption rate (3 hours reach adsorption equilibrium) and very big adsorptive capacity as can be seen from Figure 3.
Described fixed microorganism carrier bed is a modified Nano SiOx composite polyurethane foam, X=1.2～1.6.Its preparation method is to add modification SiOx nanoparticle to be prepared from the polyurethane foam foaming process.
The wetting ability microenvironment that modified Nano SiOx composite polyurethane foam has will help the metabolism propagation of fixation of microbe, can increase biological charge capacity and improve biological respinse efficient; Simultaneously, because the enhancement of nanoparticle and the reinforcement of crosslinking structure improved and the chemistry and the physical stability that improve the gained foam carrier is given its good impact resistance.
The pond body of two-phase distributing bioreactor of the present invention is a rectangle or cylindrical, and the quantity of reactor internal fixing microbe carrier bed, solid phase extraction agent bed can be according to the actual needs of sewage treatment process, by the setting and the adjustment of filter screen.The size of filter screen mesh also can be adjusted according to the volume size of macropore microbe carrier and solid phase extraction agent.
Two-phase distributing bioreactor of the present invention can constitute different type of reactor according to the different sorts of the immobilized microorganism that adds in the reactor and the different performance of extraction agent.
Two-phase distributing bioreactor of the present invention, be used to handle high concentrated organic wastewater: utilize sump pump that the water inlet pipe of high concentrated organic wastewater process reactor bottom is entered two-phase distributing bioreactor, when waste water is flowed through solid extracting agent on the filter screen, organic pollutant reaches balance at water and solid phase, and then has reduced the organic contamination substrate concentration in the water; When the waste water of end concentration is flowed through macropore carrier filler and immobilized microorganism thereof, carry out biochemical treatment, open aerating system simultaneously and make the micropore of air on aeration tube bloat the aerobic industrial and mineral of formation; Under the dual promotion of water flow pressure and aeration pressure, treat effluent is flowed out from rising pipe.
Two-phase distributing bioreactor of the present invention is applied to the processing of high density and bio-toxicity organic waste water, hydrophobic contaminant can be dissolved in the non-aqueous solution phase (solid phase) in the two-phase distributing bioreactor Waste Water Treatment preferably, and the solvability in its water-soluble liquid phase (liquid phase) is relatively poor.Bio-degradation reactions is carried out in water-soluble liquid phase or two-phase interface, and pollutent is delivered to water-soluble liquid phase by the solid extracting agent bed by two-phase interface and reacts, and the pollutent wear rate then can be by the relative size control of its transfer rate and biological reactions rates.The solid extracting agent bed of two-phase distributing bioreactor serves as the enricher and the storage vault of pollutent, and one side increases the pollutant dissolution degree, can realize the biological respinse of high concentration of substrate; The poisoning level that the Pollutant levels of biological respinse phase is maintained be lower than microorganism by slow releasing function has been removed pollutent to its biodegradable restraining effect on the other hand.
The present invention has following advantage compared to existing technology:
1, two-phase distributing bioreactor of the present invention is introduced two-phase distributing bioreactor with the macropore carrier immobilized microorganism, makes it have better biological tolerance; Introduce nonyl alcohol/polysulfones and two kinds of solid phase extraction agent of organo montmorillonite/polysulfones, with solve organic extraction mutually biodegradable, suppress that a large amount of foams of generation cause problems such as microorganism loss in microorganism growth and the aeration process; The adsorptive capacity of solid phase extraction agent simultaneously is big, the adsorption rate height, and available mixed bacterial is handled complicated waste water.
2, the present invention adopts solid phase polymer extraction agent, dynamichandling waste water continuously.
3, the relative liquid extractant of solid-state polymer extraction agent of the present invention is cheap, effectively reduces the cost of reactor.
Description of drawings
Fig. 1 is the structural representation of two-phase distributing bioreactor of the present invention
1---outlet valve 2---rising pipe 3---filter screen 4---solid phase extraction agent bed
5---microbe carrier bed 6---inlet pipe that water pipe 7 exposes to the sun---aeration tube 8---
9---air valve 10---base 11---water inlet pipe 12---water intaking valves
Fig. 2 is the nonyl alcohol/polysulfones extraction agent Pyrogentisinic Acid's of the present invention's preparation adsorption curve
Fig. 3 is machine polynite under the different initial phenol concentration/polysulfones extraction agent Pyrogentisinic Acid's adsorptive capacity and a time relation
Fig. 4 is the static degradation curve of two-phase distributing bioreactor to the high concentration phenol artificial wastewater
Embodiment
A kind of two-phase distributing bioreactor comprises pond body 13, is provided with filter screen 3 in the body of pond, has been arranged alternately solid extracting agent bed 4 and fixed microorganism carrier bed 5 between each filter screen from bottom to top; Be provided with expose to the sun water system and aerating system in body bottom, pond; Be provided with rising pipe 2 on body top, pond.Wherein body 13 bottoms in pond are provided with base 10; The water system that exposes to the sun is made up of expose to the sun water pipe 6 and water inlet pipe 11, water intaking valve 12; Aerating system is made up of aeration tube 7, inlet pipe 8, air valve 9.With reference to Fig. 1.
The aperture of filter screen 3 should be controlled in the scope of 1～2mm.
The solid extracting agent of solid extracting agent bed 4 is the solid extracting agent that polysulfones embedding nonyl alcohol or polysulfones embedding organo montmorillonite form.
Wherein polysulfones embedding nonyl alcohol forms the preparation method of solid extracting agent, be under the mechanical stirring condition, polysulfones is dissolved in the N-N-methyl-2-2-pyrrolidone N-with 1: 5～1: 10 bulking value, after the nonyl alcohol that adding polysulfones quality is 3～4 times stirs, splash into the needle tubing that has the 12# syringe needle and form the plate-like bead in the Span-80 aqueous solution of 0.1～0.3wt.%, and after in distilled water, soaking 24h, dry and get final product.
Polysulfones embedding organo montmorillonite forms the preparation method of solid extracting agent, may further comprise the steps:
(1) Organophilic Modification of Montmorillonite: with the W for preparing
MMTThe polynite of=0.01-0.1wt.% (MMT) slurries and W
CTABThe hexadecyl trimethyl ammonium bromide of=0.007～0.03wt.% (CTAB) solution mixes with 1: 1～1: 3 volume ratio, stirs 4h down at 80 ℃, is cooled to after the room temperature with No. three husky heart funnel suction filtrations, is washed till no Br with distilled water and (uses the AgNO of 0.1mol/L
3Solution detects no flocks), vacuum-drying was ground 200 mesh sieves and was obtained organic montmorillonoid (OMMT) to constant weight.
(2) be under mechanical stirring to be dissolved in N-N-methyl-2-2-pyrrolidone N-with 1: 10～1: 15 weightmeasurement ratio polysulfones, the organo montmorillonite that adds 0.2～1.5 times of polysulfones quality, stir the back under mechanical stirring, splash into the needle tubing that has the 12# syringe needle in the ethanol of mass concentration 15～35%, form globe, and globe is soaked the 24h final vacuum be drying to obtain the solid phase extraction agent in distilled water.
Fixed microorganism carrier bed 5 is a modified Nano SiOx composite polyurethane foam, X=1.2～1.6.Its preparation method is to add modification SiOx nanoparticle to be prepared from the polyurethane foam foaming process.Chinese invention patent ZL 200310121064.9 is seen in concrete preparation.
Embodiment 2, utilize two-phase to distribute reactor for treatment high concentration phenol waste water
The static degradation experiment of phenol artificial wastewater in two-phase distributing bioreactor, giving 2L concentration is that the organo montmorillonite/polysulfones bead that adds 100g in the phenol solution of 4793.1mg/l makes it reach balance, add then and fix microorganism carrier (PUF/IM) 28.4g (dry weight), aeration, the test of degrading.Process of the test is regulated the pH value, makes it remain on neutrality, and the nutritive salt timely and appropriate discovery replenishes.
Controlled trial group: make it reach balance for the organo montmorillonite/polysulfones bead that adds 100g in the phenol solution of 2L 4793.1mg/l, add carrier (PUF) 13.2g then, also the while aeration.
Fig. 4 is the static degradation curve of two-phase distributing bioreactor to the high concentration phenol artificial wastewater.As can be seen from Figure 4, at 0～7h, organo montmorillonite/polysulfones bead adsorbing phenol reaches balance, and the concentration during balance is about 1500mg/L; The 7th hour, adding fixed microorganism carrier; 7～8 hours, existing PUF carrier Pyrogentisinic Acid's absorption has the degraded of microorganism again, but absorption is main, this is because PUF Pyrogentisinic Acid's adsorption rate is exceedingly fast, and 30min just can adsorption equilibrium, and the PUF/IM carrier that is fixed with microorganism has at this moment just added in this individual system, in order to adapt to new system, microorganism has a lag period, and breeding of microorganism and Pyrogentisinic Acid's degradation rate all compare at the end at this moment, last till this period the 10th hour always; 10～18 hours, the microorganism of this moment adapted to this individual system fully, and the Pyrogentisinic Acid has maximum degradation rate; Since the 18th hour, though microorganism has very high activity, and more quantity is arranged, but the speed that this moment, organo montmorillonite/polysulfones bead and PUF carrier Pyrogentisinic Acid discharged is far away not as good as the degradation rate of microorganism to it, therefore, the degradation rate of this process phenol is the rate-controlling that discharged by organo montmorillonite/polysulfones bead and PUF carrier Pyrogentisinic Acid.Finally, sharp two-phase distributing bioreactor of the present invention is that the phenol artificial wastewater's of 4793.1mg/l degradation rate has reached 342.4mg/L*h to initial concentration.
Claims (6)
1. a two-phase distributing bioreactor comprises pond body (13), it is characterized in that: be provided with filter screen (3) in the body of pond, circulation from bottom to top is provided with solid extracting agent bed (4) and fixed microorganism carrier bed (5) between each filter screen; Be provided with expose to the sun water system and aerating system in body bottom, pond; Be provided with rising pipe (2) on body top, pond.
2. two-phase distributing bioreactor as claimed in claim 1 is characterized in that: the solid extracting agent of described solid extracting agent bed is the solid extracting agent that polysulfones embedding nonyl alcohol or polysulfones embedding organo montmorillonite form.
3. two-phase distributing bioreactor as claimed in claim 1 is characterized in that: described fixed microorganism carrier bed is a modified Nano SiOx composite polyurethane foam, X=1.2～1.6.
4. two-phase distributing bioreactor as claimed in claim 2, it is characterized in that: described polysulfones embedding nonyl alcohol forms the preparation method of solid extracting agent, be under the mechanical stirring condition, polysulfones is dissolved in the N-N-methyl-2-2-pyrrolidone N-with 1: 5～1: 10 weightmeasurement ratio, after the nonyl alcohol that adding polysulfones quality is 3～4 times stirs, splash into the needle tubing that has the 12# syringe needle and form the plate-like bead in the Span-80 aqueous solution of 0.1～0.3wt.%, and after in distilled water, soaking 24h, dry and get final product.
5. two-phase distributing bioreactor as claimed in claim 2, it is characterized in that: described polysulfones embedding organo montmorillonite forms the preparation method of solid extracting agent, be under mechanical stirring to be dissolved in N-N-methyl-2-2-pyrrolidone N-with 1: 10～1: 15 weightmeasurement ratio polysulfones, the organo montmorillonite that adds 0.2～1.5 times of polysulfones quality stirs, under agitation splash in the ethanol of mass concentration 15～35% with the needle tubing that has the 12# syringe needle, form globe, and globe is soaked the 24h final vacuum be drying to obtain the solid phase extraction agent in distilled water.
6. two-phase distributing bioreactor as claimed in claim 1 is used to handle high concentration hard-degraded organic waste water.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649410A (en) * | 2013-11-26 | 2015-05-27 | 汉尼科(南京)环保有限公司 | Double-carrier fixed bed biomembrane reactor |
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| CA2247779A1 (en) * | 1997-09-19 | 1999-03-19 | Andrew J. Daugulis | Process for biodegradation of a xenobiotic |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649410A (en) * | 2013-11-26 | 2015-05-27 | 汉尼科(南京)环保有限公司 | Double-carrier fixed bed biomembrane reactor |
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