CN101684448A - Streptomyces diastochromogenes, fermentation product and application thereof - Google Patents
Streptomyces diastochromogenes, fermentation product and application thereof Download PDFInfo
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- CN101684448A CN101684448A CN 200810200613 CN200810200613A CN101684448A CN 101684448 A CN101684448 A CN 101684448A CN 200810200613 CN200810200613 CN 200810200613 CN 200810200613 A CN200810200613 A CN 200810200613A CN 101684448 A CN101684448 A CN 101684448A
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Abstract
The invention discloses a Streptomyces diastochromogenes, a fermentation product 5'-epi-SPA-6952A and an application thereof. The Streptomyces diastochromogenes of the invention has the preservation number of CGMCCNo.2281, the fermentation product thereof has favourable insecticidal activity, and the active ingredient with insecticidal activity in the fermentation product is 5'-epi-SPA-6952A. Thefermentation liquor and the active ingredient 5'-epi-SPA-6952A of the Streptomyces diastochromogenes of the invention both have favourable insecticidal activity and low production cost, is friendly toenvironment and has favourable application prospect. In addition, the compound 5'-epi-SPA-6952A is a new compound, thus having wide development and application prospect.
Description
Technical field
The invention belongs to microbial technology field, specifically, is about a kind of streptomyces diastatochromogenes (Streptomycesdiastochromogenes) SSPRC-11339, its tunning and application.
Background technology
The chemical pesticide control insect pest has played crucial effect for the development of agriculture production.But, make pest control be tending towards complicated and difficult because the long-term chemical pesticide that relies on is ignored the nature regulation and control.And a lot of medicaments all exist a dosage big, and toxicity is higher, to problems such as environment are influential, do not meet the requirement of current social, are badly in need of having product innovation to substitute.
For effective pest control, the research and development of biological pesticide (comprising agricultural antibiotic) have caused in the whole world widely to be paid attention to.In addition, along with the quickening of China joined WTO paces, will copy new external agricultural chemicals possibility hardly again, exploitation has the Pesticidal products of independent intellectual property right, has become extremely urgent task.
Along with the rise of biotechnology, the active substance that utilizes microorganism and biogenic to produce is subjected to countries in the world as the research of developing novel pesticide and payes attention to greatly.This is not pollute the environment owing to biological pesticide is easily decomposed by nature usually, produces different products, low, the reduced investment of energy consumption but also can utilize with required appointed condition much at one and the renewable resource of microorganism growth breeding.
Summary of the invention
In order to seek more more effective sterilant than in the past, present inventor's separate microorganism and produce its meta-bolites from multiple soil, then the insecticidal activity of the meta-bolites that obtains is studied, the result has found a strain bacterial strain SSPRC-11339, and its meta-bolites has good prevention effect for multiple farmland plant insects such as mythimna separatas.Through the physico-chemical property of SSPRC-11339 etc. is analyzed, determine that this bacterial strain is streptomyces diastatochromogenes (Streptomycesdiastochromogenes).Through the further activeconstituents that extracts in the meta-bolites, and the structure of definite activeconstituents, find that this activeconstituents is a kind of new compound.
Therefore, primary and foremost purpose of the present invention just is, a kind of streptomyces diastatochromogenes (Streptomycesdiastochromogenes) SSPRC-11339 is provided, and its meta-bolites has good insecticidal activity.
Second purpose of the present invention is, described streptomyces diastatochromogenes (Streptomycesdiastochromogenes) application that SSPRC-11339 is used to prepare agricultural insecticide is provided.
The 3rd purpose of the present invention is, a kind of new compound 5`-epi-SPA-6952A that is obtained by described streptomyces diastatochromogenes (Streptomycesdiastochromogenes) SSPRC-11339 fermentation is provided.
The 4th purpose of the present invention is, a kind of agricultural insecticide is provided.
The 5th purpose of the present invention is, the application of described agricultural insecticide is provided.
Described streptomyces diastatochromogenes (Streptomyces diastochromogenes) SSPRC-11339 with insecticidal activity of the present invention, its 16srDNA sequence is shown in SEQ ID NO:1.
Further, the preserving number of described streptomyces diastatochromogenes (Streptomyces diastochromogenes) SSPRC-11339 is CGMCC No.2281.
The meta-bolites of streptomyces diastatochromogenes of the present invention (Streptomyces diastochromogenes) SSPRC-11339 has good prevention effect for multiple farmland plant insects such as mythimna separatas, thereby can be used to produce agricultural insecticide.
The new compound 5`-epi-SPA-6952A that obtains by described streptomyces diastatochromogenes (Streptomyces diastochromogenes) SSPRC-11339 fermentation of the present invention, be compound S PA-6952A (Nobuo Hosotani et al.J.Antibiot.58 (6): 409-411,2005) chiral isomer, its structural formula is as follows:
Agricultural insecticide of the present invention is an activeconstituents with compound 5`-epi-SPA-6952A, can also add one or more auxiliary agents commonly used.
According to the present invention, described when being the agricultural insecticide of effective ingredient in preparation with activeconstituents 5`-epi-SPA-6952A, can use equally agricultural chemicals known in the art general various carriers such as solid phase, liquid phase and emulsifying dispersant, and can be mixed with formulation arbitrarily such as missible oil, granule, aqua, granula, wettable powder, microcapsule, suspension concentrate, aerosol.Simultaneously, in various preparations, generally some auxiliary agents can be suitably added,, but alkaline auxiliary can not be added as dispersion agent, emulsifying agent, wetting agent, permeate agent, spreader-sticker, tackiness agent, defoamer, whipping agent, tackifier, stablizer etc.The carrier that is used for preparation can be such as Jeeklite, talcum, wilkinite, clay, kaolin, diatomite, white carbon black, vermiculite, white lime, silica sand, the solid carrier of ammonium sulfate or urea etc., such as Virahol, dimethylbenzene, the liquid vehicle of hexanaphthene or methylnaphthalene etc.Tensio-active agent described here and dispersion agent can be that phenylformic acid is received, pure sulphonate, alkyl aryl sulfonate, sulfonated lignin, polyoxyethylene glycol ether, polyoxyethylene alkylaryl ether or polyoxyethylene sorbitol mono alkyl ester.Auxiliary agent can be carboxymethyl cellulose, polyoxyethylene glycol or Sudan Gum-arabic.
The proportioning of effective ingredient in the various preparations can suitably increase and decrease according to different application targets.In general, the effective ingredient scope of aqua, pulvis, wettable powder, granule is usually at 1-30%, and the effective ingredient scope of emulsion, suspension concentrate is at 0.2-10%, and microcapsule is at 10-30%.
In actual use, described agricultural insecticide can be diluted to suitable concentration before use, or directly uses.
In addition, agricultural insecticide of the present invention also can be used in combination with other sterilant.
According to the present invention, described is that activeconstituents can be used to prevent and treat mythimna separata with compound 5`-epi-SPA-6952A.
Streptomyces diastatochromogenes of the present invention (Streptomyces diastochromogenes) SSPRC-11339, because its meta-bolites has good insecticidal activity, and the cost of fermentation is low, environmentally friendly, thereby has a good application prospect.In addition, streptomyces diastatochromogenes of the present invention (Streptomyces diastochromogenes) SSPRC-11339, the activeconstituents 5`-epi-SPA-6952A in its meta-bolites is a kind of new compound, thereby has development prospect widely.
Description of drawings
Fig. 1 be bacterial strain SSPRC-11339 of the present invention based on the tree-shaped figure of the phyletic evolution of its 16srDNA sequence construct, shown the position of bacterial strain SSPRC-11339 in system takes place.
Fig. 2 is the ultraviolet absorpting spectrum of compound 5`-epi-SPA-6952A of the present invention.
Fig. 3 is the proton nmr spectra of compound 5`-epi-SPA-6952A of the present invention.
Fig. 4 is the hydrocarbon relation of the multikey of compound 5`-epi-SPA-6952A of the present invention (HMBC) collection of illustrative plates.
Fig. 5 is H-H relation (COSY) collection of illustrative plates of compound 5`-epi-SPA-6952A of the present invention.
Fig. 6 is the carbon spectrum of compound 5`-epi-SPA-6952A of the present invention.
The preservation item
Streptomyces diastatochromogenes of the present invention (Streptomyces diastochromogenes) SSPRC-11339 has submitted on December 5th, 2007 and has been positioned at Pekinese China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, preserving number: CGMCC No.2281.
Embodiment
Below in conjunction with specific embodiment, the insecticidal activity of streptomyces diastatochromogenes of the present invention (Streptomycesdiastochromogenes) SSPRC-11339 and meta-bolites thereof is described in further detail.Should be understood that following examples only are used to the present invention is described but not are used to limit scope of the present invention.
Measure through conventional method, the cultural characters of bacterial strain SSPRC-11339 of the present invention, morphological specificity, physiological and biochemical property and chemical classification and the result who analyzes according to the 16srDNA evolutionary tree are as follows:
1.1, cultural characters
| Substratum | Aerial hyphae | Substrate mycelium | Soluble pigment |
| The Gause I synthetic medium | It is cotton-shaped that brick dust becomes | Pale red partially | Do not have |
| Glucose asparagine agar | It is cotton-shaped that brick dust becomes | In vain | Do not have |
| Sucrose nitrate agar | Brick dust | In vain | Do not have |
| The inorganic salt Starch Agar | The cuttlefish ash | Colourless | Do not have |
| Oatmeal agar | Ash fine hair shape | In vain | Do not have |
| Potato is soaked juice agar | Yellow ash | In vain | Do not have |
| The yeast wort soaks juice agar | Dark cuttlefish ash | Shallow mango palm fibre | The bamboo shoot palm fibre |
| Sang Tasi agar | Ash | In vain | Brown black |
1.2, morphological specificity
Substratum: Gao Shi
Substrate mycelium: the tabula non-cracking does not have conidium and does not have
Aerial mycelium: branch's growth branch
Fibrillae of spores: length loose spiral end 2～3 ring tops
Spore: there is depression on the long plant type of shape surface
1.3, physiological and biochemical property and chemical classification
The physio-biochemical characteristics result of bacterial strain SSPRC-11339 is: gelatin does not liquefy, and slightly solidifies in the skimmed milk in the skimmed milk and peptonizes, and the starch hydrolysis is complete, H
2S produces positive, and the carbon source that can utilize is D-glucose, D-wood sugar, D-N.F,USP MANNITOL, L-inositol, L-rhamnosyl, raffinose, L-rhamnosyl, sucrose, D-fructose; Can not utilize Mierocrystalline cellulose, L-arabinose physio-biochemical characteristics and streptomyces approaching.
1.4, the 16srDNA evolutionary tree analyzes
The 16srDNA nucleotide sequence total length of bacterial strain SSPRC-11339 is 1477bp, and concrete sequence is shown in SEQ ID No:1.
Fig. 1 is based on the tree-shaped figure of the phylogeny of 16srDNA sequence, has shown the position of bacterial strain SSPRC-11339 in system takes place.The numerical value of each node (percentage ratio) is the Bootsrap value of 1000 duplicate sampling checks.
Bacterial strain SSPRC-11339 is well-grown on most of substratum, and on the Gao Shi substratum, bacterium colony is neat, and substrate mycelium does not have tabula, do not rupture, aerial mycelium branch, it is rich to grow, and is loose spiral end 2～3 rings, spore long column shape, there is depression on the surface, belongs to streptomyces.From the tree-shaped figure of phylogeny, producing the look streptomycete poly-with starch is one.Through consulting uncle Jie Shi handbook, it is approaching that its Physiology and biochemistry character and starch produce the look streptomycete.
According to above morphologic observation and physiological and biochemical property, analyze in conjunction with the 16srDNA evolutionary tree, determine that bacterial classification SSPRC-11339 should belong to streptomyces Streptomyces diastochromogenes (starch produces the look streptomycete).
Seed culture medium: glucose 2%, starch 5%, groundnut meal 2%, soybean cake powder 1%, peptone 0.1%, yeast extract paste 0.4%, sodium-chlor 0.2%, sal epsom 0.5%, ammonium nitrate 0.2%, dipotassium hydrogen phosphate 0.05%, pH 7.2.
Fermention medium: with the seed substratum.
By above formulated seed culture medium, the bacterial classification 11399 that will grow in the Gao Shi inclined-plane then is inoculated in the substratum of preparation, cultivates 36～40 hours down at 28 ℃, obtains seed liquor.
Get the seed liquor of above-mentioned acquisition, the ratio with 5～20% is inoculated in the fermentation culture of above-mentioned substratum composition, and 28 ℃ of aeration-agitations were cultivated 90～96 hours, obtained fermented liquid.
Get 10 kilograms of the fermentation cultures that embodiment 1 obtains, filter, get 8 kilograms of supernatant liquors, handle with membrane filtration to PH4～5 backs with hcl acidifying, get 10 kilograms of feed liquids, adsorb with 1 kilogram of macroporous adsorbent resin 1300 type then, (alcohol: water=1: 1) (volume ratio) carries out wash-out with 3 kilograms of alcohol waters, the elutriant concentrating under reduced pressure, obtain 45 gram brown solid thing I, (methyl alcohol: (volume ratio) dissolving water=90: 10), placement is spent the night in the refrigerator, gets 10 gram white solid II to use the 400ml methanol-water then.
3.1, the mensuration of ultraviolet absorpting spectrum
The Compound I I 0.5mg that gets embodiment 2 acquisitions is dissolved in the 50ml methyl alcohol, getting 3ml adds in the cell, make blank with methyl alcohol, at ultraviolet full wavelength scanner instrument (UV-2501PC, day island proper Tianjin company) measures on, measure wavelength from 400nm to 200nm, obtain the ultraviolet absorpting spectrum (Fig. 2) of this compound.
By the ultraviolet absorpting spectrum of Fig. 2 as seen, this Compound I I has located absorption endways.
3.2, nuclear magnetic resonance hydrogen spectruming determining
Get the Compound I I 10mg that embodiment 2 obtains, be dissolved in the 0.5ml deuterochloroform, with tetramethylsilane TMS is the primary standard of measuring chemical shift, in the enterprising line scanning of VARIAN 500MHz nuclear magnetic resonance analyser (VARIAN COMPANY), obtains this compound proton nmr spectra (Fig. 3).
As seen from Figure 3, the molecular weight of this compound is 894.
3.3, the hydrocarbon relevant spectrum of nucleus magnetic resonance (HMQC and COSY) measures
Get the Compound I I 10mg that embodiment 2 obtains, be dissolved among the 0.5mlDMSO, with tetramethylsilane TMS is the primary standard of measuring chemical shift, in the enterprising line scanning of VARIAN 500MHz nuclear magnetic resonance analyser (VARIAN COMPANY), obtain the hydrocarbon relation of multikey (HMBC) and (Fig. 4) concern (COSY) (Fig. 5) with H-H, according to HMBC and COSY collection of illustrative plates, obtain corresponding charts data (table 1).
Table 1,
1H and
13C NMR Data (in CD
3OD).
By the result of table 1 as seen, the space structure relation that meets this compound carbon-hydrogen and carbon-to-carbon.
3.4, carbon spectrum measures
Get the Compound I I 10mg that embodiment 2 obtains, be dissolved among the 0.5ml DMSO, with tetramethylsilane TMS is the primary standard of measuring chemical shift, in the enterprising line scanning of VARIAN 500MHz nuclear magnetic resonance analyser (VARIAN COMPANY), obtains the carbon spectrum (Fig. 6) of Compound I I.
As seen from Figure 6, this compound has 49 carbon.
According to the analytical results of above each collection of illustrative plates, the activeconstituents (Compound I I) that can draw in the meta-bolites of streptomyces diastatochromogenes of the present invention (Streptomycesdiastochromogenes) SSPRC-11339 has following structural formula:
This compound and existing compound S PA-6952A (the Nobuo Hosotani et al.J.Antibiot.58 (6): 409-411 that reports, 2005) structure is basic identical, difference is the isomerization of methyl on the 5` position, it is the chiral isomer of compound S PA-6952A, called after 5`-epi-SPA-6952A, be a kind of new compound, molecular formula is: C
49H
83NO
13Molecular weight is 894.
4.1, compound 5`-epi-SPA-6952A makes wettable powder
Get 40 parts of (weight ratios of compound 5`-epi-SPA-6952A (Compound I I) that embodiment 3 obtains, down with) draw back powder, 2 parts for the treatment of compound LS and 52 parts of diatomite and mix with 2 parts of sodium lignosulfonates, 4 parts, pulverize, promptly obtain 100 parts of content and be 40% wettable powder.
4.2, compound 5`-epi-SPA-6952A makes missible oil
Get 28 parts of compound 5`-epi-SPA-6952A (Compound I I) that embodiment 3 obtains and mix, promptly obtain 100 parts of content and be 28% missible oil with 10 parts of ethylene glycol, 20 parts of dimethyl formamides, 10 parts of dimethylbenzene and 32 parts of ethanol.
Get the fermented liquid supernatant liquid that embodiment 1 obtained, the compound 5`-epi-SPA-6952A (Compound I I) that embodiment 2 obtains, the wettable powder and the missible oil of embodiment 4 preparations respectively, be diluted to each predetermined concentration shown in the table 2, simultaneously in contrast with clear water, with the mythimna separata is object, using mythimna separata to soak the leaf feeding method tests, each processing is provided with 3 repetitions, and is specific as follows:
The leaf of Semen Maydis section of clip 2 * 5cm is flooded 10s with blade in soup, dry in the shade after the taking-up.Handle blank earlier, repeat aforesaid operations by test design dosage order from low to high then, every processing repeats for 3 times.Select neat and consistent two age mythimna separata be inoculated in and anti-have in the culture dish of maize leaf, preserve moisture with two-layer gauze covering above.Examination worm after handling placed respectively under the normal condition raise; Every 24h observes examination worm response situation, inquiry test-results during 48h.With the Abbott formula (specifically use the dead worm rate of Abbott formula calculation correction p, its calculation formula is: p=(p '-c)/(1-c), p ' is not for proofreading and correct dead worm rate, c is the dead worm rate of control group.By standard GB 13917.1～13917.8-92, c should be greater than 20%.) calculating the mortality ratio that each is handled, the result is as shown in table 2.
The insecticidal activity assay of table 2, compound 5`-epi-SPA-6952A
Annotate: carry out the statistical computation of mortality ratio behind the living 48h of survey, can the worm that live normally creep with the shake-up polypide with the discriminating of dead worm and be as the criterion, and it is dead individual that improper unnatural, half-dead, the complete dead individuality of promptly creeping is all calculated.
By the result of table 2 as seen, the fermented liquid of streptomyces diastatochromogenes of the present invention (Streptomyces diastochromogenes) SSPRC-11339 and the activeconstituents 5`-epi-SPA-6952A of extraction have good insecticidal activity, and production cost is low, environmentally friendly, thereby have a good application prospect; Compound 5`-epi-SPA-6952A is a kind of new compound in addition, and not seeing in the prior art has report, therefore has the excellent development application prospect.
Sequence table
＜110〉Shanghai Pesticide Research Institute Shanghai Nanfang Pesticide Research Centre
＜120〉a kind of streptomyces diastatochromogenes, its tunning and application
<130>P5081067
<160>1
<170>PatentIn?version?3.3
<210>1
<211>1477
<212>DNA
<213>Streptomyces?diastochromogenes
<220>
<221>16srDNA
<222>(1)..(1477)
<400>1
gacgaacgct?ggcggcgtgc?ttaacacatg?caagtcgaac?gatgaaccac?ttcggtgggg?????60
attagtggcg?aacgggtgag?taacacgtgg?gcaatctgcc?cttcactctg?ggacaagccc????120
tggaaacggg?gtctaatacc?ggatacaacc?accggccgca?tggtctggtg?gtggaaagct????180
ccggcggtga?aggatgagcc?cgcggcctat?cagcttgttg?gtgaggtaac?ggctcaccaa????240
ggcgacgacg?ggtagccggc?ctgagagggc?gaccggccac?actgggactg?agacacggcc????300
cagactccta?cgggaggcag?cagtggggaa?tattgcacaa?tgggcgaaag?cctgatgcag????360
cgacgccgcg?tgagggatga?cggccttcgg?gttgtaaacc?tctttcagca?gggaagaagc????420
gaaagtgacg?gtacctgcag?aagaagcgcc?ggctaactac?gtgccagcag?ccgcggtaat????480
acgtagggcg?cgagcgttgt?ccggaattat?tgggcgtaaa?gagctcgtat?gcggtctgtc????540
gcgtcggatg?tgaaagcccg?gggcttaacc?ccgggtctgc?attcgatacg?ggcagactac????600
agtgtggtag?gggagatcgg?aattcctggt?gtagcggtga?aatgcgcaga?tatcaggagg????660
aacaccggtg?gcgaaggcgg?atctctgggc?cattactgac?gctgaggagc?gaaagcgtgg?????720
ggagcgaaca?ggattagata?ccctggtagt?ccacgccgta?aacggtggga?actaggtgtt?????780
ggcgacattc?cacgtcgtcc?gtgccgcagc?taacgcatta?agttccccgc?ctggggagta?????840
cggccgcaag?gctaaaactc?aaaggaattg?acgggggccc?gcacaagcag?cggagcatgt?????900
ggcttaattc?gacgcaacgc?gaagaacctt?tacacgcttg?acatacaccg?gaaacggcca?????960
gagatggtcg?cccccttgtg?gtcggtgtac?aggtggtgca?tggctgtcgt?cagctcgtgt????1020
cgtgagatgt?tgggttaagt?cccgcaacga?gcgcaaccct?tgttctgtgt?tgccagcatg????1080
cccttcgggg?tgatggggac?tcacaggaga?ctgccggggt?caactcggag?gaaggtgggg????1140
acgacgtcaa?gtcatcatgc?cccttatgtc?ttgggctgca?cacgtgctac?aatggcaggt????1200
acaatgagct?gcgaagccgt?gaggcggagc?gaatctcaaa?aagcctgtct?cagttcggat????1260
tggggtctgc?aactcgaccc?catgaagtcg?gagttgctag?taatcgcaga?tcagcattgc????1320
tgcggtgaat?acgttcccgg?gccttgtaca?caccgcccgt?cacgtcacga?aagtcggtaa????1380
cacccgaagc?cggtggccca?acccgtaagg?gagggagctg?tcgaaggtgg?gactggcgat????1440
tgggacgaag?tcgtaacaag?gtagccgtac?cggaagg?????????????????????????????1477
Claims (10)
1, a kind of streptomyces diastatochromogenes (Streptomyces diastochromogenes) is characterized in that, the 16srDNA sequence of this bacterial strain is shown in SEQ ID NO:1.
2, streptomyces diastatochromogenes as claimed in claim 1 (Streptomyces diastochromogenes) is characterized in that, the preserving number of this bacterial strain is CGMCC No.2281.
3, the application of streptomyces diastatochromogenes as claimed in claim 1 or 2 (Streptomyces diastochromogenes) is characterized in that, is used to produce agricultural insecticide.
4, a kind of compound that is obtained by claim 1 or 2 described streptomyces diastatochromogenes (Streptomyces diastochromogenes) fermentation is characterized in that described compound has following structural formula:
5, application of compound as claimed in claim 4 is characterized in that, is used to prepare agricultural insecticide.
6, a kind of agricultural insecticide is characterized in that, this agricultural insecticide is an activeconstituents with the described compound of claim 4.
7, agricultural insecticide as claimed in claim 6 is characterized in that, also comprises one or more auxiliary agents.
8, agricultural insecticide as claimed in claim 7 is characterized in that, described auxiliary agent is selected from: dispersion agent, emulsifying agent, wetting agent, permeate agent, spreader-sticker, tackiness agent, defoamer, whipping agent, tackifier, stablizer.
9, as each described agricultural insecticide among the claim 6-8, it is characterized in that the formulation of described agricultural insecticide is selected from: missible oil, granule, aqua, granula, wettable powder, microcapsule, suspension concentrate, aerosol.
10, as the application of each described agricultural insecticide among the claim 6-9, it is characterized in that, be used to prevent and treat mythimna separata.
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| CN 200810200613 CN101684448B (en) | 2008-09-27 | 2008-09-27 | Streptomyces diastochromogenes, fermentation product and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103289945A (en) * | 2013-05-08 | 2013-09-11 | 中国计量学院 | Frr expression reinforced recombined streptomyces diastatochromogenes, as well as construction method and use |
| CN105994304A (en) * | 2016-05-09 | 2016-10-12 | 华东理工大学 | Application of novel insect cell immunosuppressor |
| CN112640917A (en) * | 2020-12-14 | 2021-04-13 | 中国计量大学 | Application of streptomyces diastatochromogenes metabolite in preventing and treating thunberg dwarf lilyturf black spot and anthracnose |
| CN114164137A (en) * | 2021-10-15 | 2022-03-11 | 中国热带农业科学院热带生物技术研究所 | Streptomyces diastatochromogenes capable of resisting banana wilt and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103333909B (en) * | 2013-05-08 | 2014-12-03 | 中国计量学院 | Streptomyces diastatochromogenes electroporation transformation method |
| CN103289946A (en) * | 2013-05-08 | 2013-09-11 | 中国计量学院 | Establishment method of streptomyces diastatochromogenes expression system |
| CN103960289B (en) * | 2014-03-31 | 2016-01-20 | 中国计量学院 | A kind of preparation method preventing and treating the mixing formula preparation of brown planthopper |
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| GB8811036D0 (en) * | 1988-05-10 | 1988-06-15 | Glaxo Group Ltd | Chemical compounds |
| CN1026207C (en) * | 1988-07-02 | 1994-10-19 | 上海市农药研究所 | Compound preparation of novel liuyangmeisu (Macro tetrolide ) |
| US6682925B1 (en) * | 2000-04-13 | 2004-01-27 | Agraquest, Inc. | Streptomyces strain with insecticidal activity and method of using as an insecticide |
| JP2004210648A (en) * | 2002-12-27 | 2004-07-29 | Sumitomo Pharmaceut Co Ltd | Novel macrolide compound and method for producing the same |
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2008
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289945A (en) * | 2013-05-08 | 2013-09-11 | 中国计量学院 | Frr expression reinforced recombined streptomyces diastatochromogenes, as well as construction method and use |
| CN105994304A (en) * | 2016-05-09 | 2016-10-12 | 华东理工大学 | Application of novel insect cell immunosuppressor |
| CN105994304B (en) * | 2016-05-09 | 2020-04-03 | 华东理工大学 | Application of insect cell immunosuppressant |
| CN112640917A (en) * | 2020-12-14 | 2021-04-13 | 中国计量大学 | Application of streptomyces diastatochromogenes metabolite in preventing and treating thunberg dwarf lilyturf black spot and anthracnose |
| CN112640917B (en) * | 2020-12-14 | 2021-08-17 | 中国计量大学 | Application of streptomyces diastatochromogenes metabolite in preventing and treating thunberg dwarf lilyturf black spot and anthracnose |
| CN114164137A (en) * | 2021-10-15 | 2022-03-11 | 中国热带农业科学院热带生物技术研究所 | Streptomyces diastatochromogenes capable of resisting banana wilt and application thereof |
| CN114164137B (en) * | 2021-10-15 | 2023-06-13 | 中国热带农业科学院热带生物技术研究所 | Streptomyces diastochromogenes for resisting banana vascular wilt and application thereof |
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| Publication number | Publication date |
|---|---|
| CN102911227B (en) | 2015-08-12 |
| CN101684448B (en) | 2013-01-30 |
| CN102911227A (en) | 2013-02-06 |
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