CN109868242A - One plant of salt tolerant produces bacillus subtilis and its application of 3-hydroxy-2-butanone - Google Patents
One plant of salt tolerant produces bacillus subtilis and its application of 3-hydroxy-2-butanone Download PDFInfo
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- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 63
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 63
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- 150000003839 salts Chemical class 0.000 title claims abstract description 34
- 241000219793 Trifolium Species 0.000 claims abstract description 34
- 230000001580 bacterial effect Effects 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims abstract description 20
- 244000005700 microbiome Species 0.000 claims abstract description 6
- 239000002689 soil Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 230000015784 hyperosmotic salinity response Effects 0.000 claims description 5
- 230000008635 plant growth Effects 0.000 claims description 5
- 230000001737 promoting effect Effects 0.000 claims description 5
- 244000025254 Cannabis sativa Species 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract description 2
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- 238000000855 fermentation Methods 0.000 description 17
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- 230000000694 effects Effects 0.000 description 7
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- 210000003608 fece Anatomy 0.000 description 4
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
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- 229920002472 Starch Polymers 0.000 description 2
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses the bacillus subtilises that one plant of salt tolerant produces 3-hydroxy-2-butanone, its classification naming is bacillus subtilis (Bacillus subtilis), bacterial strain number is NRCB002, it has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, its deposit number is CGMCC NO.17213, and the deposit date is on January 18th, 2019.The invention also discloses the applications of above-mentioned bacillus subtilis.Bacillus subtilis bacterial strain NRCB002 of the invention experiments have shown that it can generate 3-hydroxy-2-butanone with salt-tolerant trait, and can promote the growth of clover, is had a good application prospect by pure culture test, laboratory flat plate test and greenhouse pot culture.
Description
Technical field
The invention belongs to microorganisms technical fields, are related to bacillus subtilis and its are promoting the application in plant growth.
Background technique
At present inside agricultural production, due to the unreasonable use for a long time of the agricultural input products such as chemical fertilizer, farmland soil is not only caused
The problems such as earth acidification, hardened, secondary salinization, is on the rise, and also destroys rhizospheric microflora of soil structure of community, there is many
Beneficial microorganism becomes secondary flora from dominant microflora.Alfalfa (Medicago sativa L.) be distributed in the world it is most wide
Perennial legume forages are known as the title of " King of Pasture ".The cultivated area of China clover is about 377.5 ten thousand hm2, but clover produces
Measure it is low, every year will be from external import clover to meet the needs of domestic animal husbandry.Salt tolerance of the clover with middle intensity, but with
The continuous aggravation of saline Land degree, the planting range of alfalfa gradually reduce.Therefore the salt resistance ability of clover is improved
It is not only the important method in modified utilization salt-soda soil, and expands the fundamental way that alfalfa cultivation range improves alfalfa output.
Plant rhizosphere growth-promoting bacterium (PGPR) has very strong colonization ability in plant root, while can promote the life of crop
It is long, the germination percentage of seed is such as improved, root growth is promoted and increases the yield of crop.Now from rice, wheat, corn,
The isolated a large amount of PRPG bacterial strain of the rhizosphere of the various crops such as peanut, and it is widely used in microbial manure and microbial bacteria
Agent field.Wherein bacillus is most widely used because of its good environmental suitability.Microbial manure and microbial bacterial agent exist
It generally requires to be applied in root or root dipping processing when application, the reason is that the growth-promoting mechanism of general bacillus are as follows: 1, raising work
The utilizability of object rhizosphere nutrient, such as Soluble phosphorus, potassium decomposing；2, plant hormone is secreted, such as heteroauxin (IAA), gibberellin (GAs)
Deng；3, few people refer to that bacterial strain generates volatile compound and can promote plant growth, and volatile materials can not contact
It plays a role in the case where root system.Have general bacillus growth-promoting feature if can obtain and generate a certain amount of volatility object
The bacterial strain of matter expands microbial manure and range when microbial bacterial agent is applied apart from root system for effective, have efficiently, safety,
Apply the advantages that facilitating.
Patent CN102827793A discloses the application method of the clover pseudomonad of one plant of production acc deaminase, feature
It is that the clover pseudomonad for producing acc deaminase can be improved the resistance of clover and promote alfalfa growing, but institute in its patent
State soil salt content only 0.037%.Patent CN201611205485.3 discloses a kind of application method of clover special bio fertilizer,
Its characteristic value is that its Special fertilizer of alfalfa contains the bacterial strains such as bacillus subtilis, although improving the yield of clover,
But the growth for promoting salt-soda soil clover is not referred to.Patent CN105296381 A discloses a bacillus subtilis CYY-25
And its method of application, it is characterised in that the growth of salt-soda soil clover, but its salt content for not being described saline-alkali soil can be promoted,
And its operation is comparatively laborious, during alfalfa growing, every 7d is needed to pour a CYY-25 bacteria suspension.Though bacillus subtilis
The growth in salt-soda soil and non-salt-soda soil clover can so be promoted, still, application saline-alkali soil salt content is relatively low and operating process
It is cumbersome, it is difficult to meet the requirement of moderate and heavy salinized ground soil.
Summary of the invention
Technical problem to be solved by the invention is to provide the bacillus subtilis that one plant of salt tolerant produces 3-hydroxy-2-butanone, bacterial strain tools
There is the excellent ability for promoting the plant growths such as clover under the conditions of with high salt and weak base, promotes clover in moderate and heavy salinized
The growth of soil.
The present invention also technical problems to be solved are to provide the application of above-mentioned bacillus subtilis.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
One plant of salt tolerant produces the bacillus subtilis of 3-hydroxy-2-butanone, and classification naming is bacillus subtilis (Bacillus
Subtilis), bacterial strain number is NRCB002, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Its deposit number is CGMCC NO.17213, and the deposit date is on January 18th, 2019, preservation address was the Chaoyang District, Beijing City North Star
No. 3 Institute of Microorganism, Academia Sinica, institute of West Road 1, postcode 100101.
Wherein, it is inventor in August, 2018 from Jiangsu that the salt tolerant, which produces the bacillus subtilis NRCB002 of 3-hydroxy-2-butanone,
Isolated in the The Rhizosphere of Rice soil of the town province Yixing City great Pu, the bacterium colony is rounded, opaque, rare translucent, faint yellow white
And protrusion, edge is irregular, easily provokes.The 16SrDNA sequence of bacterial strain is measured, it is raw by Phylogenetic Analysis and physiology
Change identification, is determined as bacillus subtilis (Bacillus subtilis), and be named as NRCB002.The bacterium can generate indoles
Acetic acid, dissolved metals, the insoluble potassium of dissolution, this is identical as most of bacillus.In addition, the bacterium can also generate volatility
Gas 3-hydroxy-2-butanone, plays a role when thallus does not contact crop root, effectively expands microbial manure and microbial bacterial agent
Apart from the range of root system when application, have the advantages that efficient, safety, application are convenient.Bacillus subtilis bacterial strain of the invention
NRCB002 is by pure culture test, laboratory flat plate test and greenhouse pot culture experiments have shown that it can be generated with salt-tolerant trait
3-hydroxy-2-butanone, and the growth of clover can be promoted, it has a good application prospect.Other features of the invention will be specific below
Implementation section is described in detail.
Wherein, the bacillus subtilis is thermophilic salt form bacillus subtilis, and in liquid medium, the tolerance of salinity is most
High reachable 100g/L.
Wherein, the bacillus subtilis, in the soil, the tolerance of salinity reaches as high as 20g/kg.
Above-mentioned salt tolerant produces effect of the bacillus subtilis of 3-hydroxy-2-butanone in fermenting and producing 3-hydroxy-2-butanone and also protects in the present invention
Within the scope of.
The bacillus subtilis that above-mentioned salt tolerant produces 3-hydroxy-2-butanone is improving the application in the urgent ability of plant salt tolerance also in the present invention
Within protection scope.
Wherein, the plant is preferably clover.
Concrete application method is bacillus subtilis NRCB002 to be applied to salt affected soil, the salt affected soil contains can
Dissolubility salinity is up to 20g/kg.
The bacillus subtilis that above-mentioned salt tolerant produces 3-hydroxy-2-butanone is promoting the application in plant growth also to protect model in the present invention
Within enclosing.
Wherein, the plant is preferably clover.
Salt resistant character of the present invention, the salt refer to KCl, K2SO4、K2CO3、KNO3、NaCl、Na2SO4、Na2CO3
One or more of equal inorganic salts.
The utility model has the advantages that bacillus subtilis NRCB002 provided by the invention can significantly improve the salt tolerant energy of the crops such as clover
Power is 0g/kg in soluble-salt content, and bacillus subtilis NRCB002 can promote significantly clover when 15g/kg, 20g/kg
Growth be inoculated with the dry of the clover of bacterial strain NCBR002 wherein growth-promoting effect is best when soluble-salt content is 20g/kg
Weight increases by 102% compared with blank.The planting range that clover can be expanded using microbial bacterial agent provided by the invention, improves crop
Cultivated area.
Detailed description of the invention
Fig. 1 is colonial morphology of the bacillus subtilis NRCB002 on fixed nitrogen culture medium.
Fig. 2 is the salt tolerance experimental result of bacillus subtilis NRCB002.
Fig. 3 is bacillus subtilis NRCB002 3-hydroxy-2-butanone chromatography testing result, wherein A is 1g/L 3-hydroxy-2-butanone standard specimen, and B is
1/30 48h fermentation liquid.
Wherein this fermentation liquid NaCl content is 60g/L.
Specific embodiment
The technical scheme of the present invention will be explained in further detail With reference to embodiment.Embodiment only to
Illustrate technical solution of the present invention, rather than is limited；Although having been carried out with reference to the foregoing embodiments to the present invention detailed
Illustrate, for those of ordinary skill in the art, can still modify to previous embodiment, or to part
It is replaced；And these modifications or substitutions, so that corresponding technical solution is detached from claimed technical solution of the invention
Range.
The separation and screening of 1 bacterial strain NRCB002 of embodiment
10g The Rhizosphere of Rice soil is taken to be put into the conical flask equipped with 90mL salting liquid, under the conditions of 28 DEG C, 200r/min, vibration
1h is swung, is made 10-1Soil supension, take 10-1Soil supension 10mL, be put into the bottle equipped with 90mL salting liquid, be made into 10-2
Dilution, and so on.It is made into 10 respectively-3、10-4、10-5Dilution.Then 10 are pipetted respectively with pipettor-3、10-4、
10-5100 μ L of dilution be coated on fixed nitrogen solid medium, three repetitions of each gradient.In 28 DEG C of constant temperature biochemical cultivation cases
2~4d of middle culture, observes its growing state daily, bigger with oese picking in superclean bench after there is single colonie
Colony inoculation to BMS culture medium on purified, purify 5~6 generations after, with 30% glycerol stocks in -80 DEG C, for use.
The identification of 2 bacterial strain NRCB002 of embodiment
Bacterial strain mode of appearance is shown in Fig. 1.
16S rDNA identification is analyzed by 16SrDNA sequence, homologous with bacillus subtilis after BLAST in NCBI is compared
Higher, similarity 100%, according to its shape and physio-biochemical characteristics, Preliminary Identification bacterial strain NRCB002 is withered grass gemma
Bacillus.
Physiology and biochemistry property is shown in Table 1: Starch Hydrolysis, V-P reaction, gelatin liquefaction, glucose produce acid, lysozyme, methyl red
Test is the positive.
1 bacterial strain NRCB002 Physiology and biochemistry property of table
| Strain number | Starch Hydrolysis | V-P reaction | Methyl red test | Gelatin liquefaction | Glucose produces acid | Lysozyme |
| NRCB002 | + | + | + | + | + | + |
The Salt Tolerance Analysis of 3 bacillus subtilis NRCB002 of embodiment
The salt-enduring strain bacillus subtilis NRCB002 bacteria suspension that will be isolated and purified, equal amount of transferring respectively to different salt
In the fluid nutrient medium of concentration, 25~30 DEG C, 150~200r/min shaking table culture observes growing state, surveys it every the 1d time
Bacteria concentration (OD600), to determine bacterium salt tolerant degree, the salt resistant character of bacterial strain is shown in Fig. 2.As can be seen from the figure as NaCl is dense
The increase of degree, bacterial strain NRCB002OD600 are gradually increased, this may infer that bacterial strain NRCB002 is Halophiles.
The measurement of 4 bacillus subtilis NRCB002 of embodiment production escaping gas 3-hydroxy-2-butanone
Fermentation seed culture medium: using LB culture medium (yeast powder 5g/L, peptone 10g/L, NaCl10g/L), cultivates item
Part is 28 DEG C of rotary shaker, 200r/min.
Fermentation medium: yeast extract 5g/L, corn pulp 4g/L, urea 4g/L, 10~100g/ of glucose 100g/L, NaCl
L is 28 DEG C in condition of culture, inside 200r/min shaking table after culture 48h, is contained with 3-hydroxy-2-butanone in gas Chromatographic Determination fermentation liquid
Amount.
3-hydroxy-2-butanone is analyzed using Agilent 7890A GC, GC condition are as follows: and 220 DEG C of hydrogen flame detector, injector temperature
210 DEG C, carrier gas N2, temperature programming.
Bacterial strain NRCB002 produces 3-hydroxy-2-butanone ability and is shown in Table 2.
5 bacillus subtilis NRCB002 of embodiment produces the identification of heteroauxin (IAA) ability
Using the ability of Salkowski colorimetric method for determining bacterial strain NRCB002 producing IAA.
Fermentation medium: yeast powder 5g/L, peptone 10g/L, NaCl 10g~100g/L, tryptophan 1g/L.It will activation
Good bacterial strain, at 28 DEG C, cultivates 2~3d, 3 weights of every plant of bacterium by 1% inoculum concentration access fermentation medium under 200r/min
It is multiple.Fermentation liquid is centrifuged 5min in 8000r/min, takes supernatant 1mL in 4mL centrifuge tube, and 2mL color solution is added, shakes up, in room
15~20min is placed under warm dark condition, and light absorption value is measured at 530nm.The IAA solution for matching various concentration with IAA mark product, with
Sample same procedure handles and measures light absorption value, draws standard curve.Reference standard curve determines IAA content.
The case where bacillus subtilis NRCB002 production heteroauxin (IAA), is shown in Table 2.
The Soluble phosphorus activity analysis of 6 bacillus subtilis NRCB002 of embodiment
Its phosphate solubilization is measured using molybdenum antimony resistance colorimetric method.
Fermentation medium: KH2PO4, 0.2g/L；K2HPO4, 0.8g/L；MgSO4·7H2O, 0.2g/L；NaCl10g~
100g/L CaSO4·2H2O, 0.1g/L；Ca3(PO4)2, 5g/L；FeCl3, micro；Na2Mo4·2H2O, it is micro；Yeast extracts
Object, 0.5g/L；The bacterial strain of activation is accessed fermentation by 1% inoculum concentration by activated bacterial strain NRCB002 by mannitol, 20g/L.
In culture medium, at 28 DEG C, 2~3d, 3 repetitions of every plant of bacterium are cultivated under 200r/min.Fermentation liquid is centrifuged in 8000r/min, is taken
Clear liquid 5mL adds deionized water about 30mL in 50mL volumetric flask, and dinitrophenol indicator 2 is added and drips, with sodium hydroxide and dilute
Then sulfuric acid tune solution is added the anti-color developing agent 5mL of molybdenum antimony, shakes up, determine molten, the colorimetric at 700nm to micro- Huang.
The Soluble phosphorus activity of bacillus subtilis NRCB002 is shown in Table 2.
The ability of dissolving potassium of 7 bacillus subtilis NRCB002 of embodiment measures
Fermentation seed culture medium: using LB culture medium (yeast powder 5g/L, peptone 10g/L, NaCl10g/L), cultivates item
Part is 28 DEG C of rotary shaker, 200r/min.
Fermentation medium: sucrose 50g/L, MgSO4·7H2O0.5g/L, CaCl20.1g/L, FeCl30.005g/L, NaCl
10g~100g/L, feldspar in powder 1.0g/L, deionized water 1.0L, pH value 7.2~7.5 are 28 DEG C in condition of culture, 200r/
After min shaking table culture 3d, with the content of atomic absorption spectrometry fermentation liquid effective K.
The ability of dissolving potassium of bacillus subtilis NRCB002 is shown in Table 2.
As can be seen from the table, when fermentation liquid NaCl concentration is 10g/L, bacterial strain NRCB002IAA secretory volume is up to
70mg/L；When fermentation liquid NaCl concentration is 60g/L, bacterial strain NRCB002 secretes 3-hydroxy-2-butanone ability and most reaches 16g/L by force；It is fermenting
Liquid NaCl concentration be 40g/L when, bacterial strain NRCB002 have preferable amount of phosphorus dissolved ability of dissolving potassium, respectively reach 75mg/L and
7.8mg/L。
The growth-promoting characteristic of 2 bacillus subtilis NRCB002 of table
The Organic volatilization gas 3-hydroxy-2-butanone that embodiment 8 is generated by bacillus subtilis NRCB002 fermentation is to the rush of clover
Raw effect
Bacillus subtilis NRCB002 is inoculated in LB liquid to support in base, at 25~30 DEG C, 150~200r/min shaking table
Lower culture is to mid-log phase (OD600=1.0), and 4 DEG C thalline were collected by centrifugation, and thallus is resuspended with 0.86% physiological saline, for use.
Alfalfa seed surface sterilization, process are as follows: 70% ethyl alcohol impregnates 15s, and 2.5% sodium hypochlorite impregnates 15min, then uses
It aseptic water washing 7~8 times, after alfalfa seed is moved on to 1/2MS culture medium, is put into 24 DEG C of illumination boxs vernalization 2 days.It will hair
The alfalfa seed of bud moves on in a Room of the 13*13cm culture dish with partition, and every ware transplants five plants, and another room of culture medium adds
The bacterium solution (OD600=1) of the NRCB002 of the 200 sterile aqueous suspensions of μ L.Culture medium both sides are containing 0g/L, 15g/L and 20g/
The 1/2MS solid medium of LNaCl is control with 200 μ L sterile waters.Culture dish ParafilmTM is placed in 24 DEG C, 16h
Illumination, 8h dark illumination box in cultivate.After cultivating 7d, plant height, fresh weight, the dry weight of clover are measured.As can be seen from Table 3
The Organic volatilization gas generated by bacillus subtilis NRCB002 can be equal when NaCl content is 0g/L, 15g/L and 20g/L
It can promote the growth of clover significantly, wherein growth-promoting effect is best, is inoculated with the clover of the microbial inoculum when NaCl content is 15g/L
Dry weight compared with blank increase by 18.1%.
The Organic volatilization gas that 3 bacillus subtilis NRCB002 of table is generated is to clover Biological control data
Physicochemical property of the embodiment 9 for examination soil
" soil agrochemistry analysis method " according to Lu Rukun chief editor is measured, and is seen for the examination initial physicochemical property of soil
Table 4.
Physicochemical property of the table 4 for examination soil
10 bacillus subtilis NRCB002 of embodiment is in soil containing soluble-salt to the growth-promoting functions of potting clover
Bacillus subtilis NRCB002 is accessed into LB culture medium, under the conditions of 28 DEG C, 200r/min, shaking table culture, every
Bacterium solution is centrifuged in 8000r/min when OD600 value is 1, collects activated bacterial strain by 2 hours survey OD600.Bacterium will be collected
Body is cleaned with 0.86% physiological saline, is then resuspended with isometric sterile water, and bacteria suspension is made after being diluted 100 times and waits for
With.It will select in the same size, after undamaged alfalfa seed is rinsed with clear water, be put into the culture dish equipped with bacteria suspension to be measured,
Impregnate 2~3h after, move it into equipped with NaCl concentration be 13.7g/kg, 18.7g/kg and 0g/kg farmland soil flowerpot in (wherein
Filling is soil described in embodiment 9 for examination soil) 20 alfalfa seeds of every basin immigration, 4 basins are sowed in every processing, in natural lighting item
Under part, germination percentage is counted when growing 14d, it is sky with not inoculating strain NCBR002 that growth 30d, which measures plant height, leaf area, fresh weight,
White control.Experimental result is shown in Table 5.It is as can be seen from Table 5 1.3g/kg, withered grass when 15g/kg, 20g/kg in soluble-salt content
Bacillus NRCB002 can promote the growth of clover significantly, wherein when soluble-salt content is 15g/kg, growth-promoting effect
Preferably, the dry weight for being inoculated with the clover of the microbial inoculum increases by 124% compared with blank.
5 bacillus subtilis NRCB002 of table is to potting clover Biological control data
Sequence table
<110>Nanjing University of Technology
<120>one plants of salt tolerants produce bacillus subtilis and its application of 3-hydroxy-2-butanone
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1393
<212> DNA
<213>bacillus subtilis (Bacillus subtilis)
<400> 1
tacctcaccg acttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaaggc 60
ccgggaacgt attcaccgcg gcatgctgat ccgcgattac tagcgattcc agcttcacgc 120
agtcgagttg cagactgcga tccgaactga gaacagattt gtgggattgg cttaacctcg 180
cggtttcgct gccctttgtt ctgtccattg tagcacgtgt gtagcccagg tcataagggg 240
catgatgatt tgacgtcatc cccaccttcc tccggtttgt caccggcagt caccttagag 300
tgcccaactg aatgctggca actaagatca agggttgcgc tcgttgcggg acttaaccca 360
acatctcacg acacgagctg acgacaacca tgcaccacct gtcactctgc ccccgaaggg 420
gacgtcctat ctctaggatt gtcagaggat gtcaagacct ggtaaggttc ttcgcgttgc 480
ttcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc 540
agtcttgcga ccgtactccc caggcggagt gcttaatgcg ttagctgcag cactaagggg 600
cggaaacccc ctaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat 660
cctgttcgct ccccacgctt tcgctcctca gcgtcagtta cagaccagag agtcgccttc 720
gccactggtg ttcctccaca tctctacgca tttcaccgct acacgtggaa ttccactctc 780
ctcttctgca ctcaagttcc ccagtttcca atgaccctcc ccggttgagc cgggggcttt 840
cacatcagac ttaagaaacc gcctgcgagc cctttacgcc caataattcc ggacaacgct 900
tgccacctac gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttaggt 960
accgtcaagg taccgcccta ttcgaacggt acttgttctt ccctaacaac agagctttac 1020
gatccgaaaa ccttcatcac tcacgcggcg ttgctccgtc agactttcgt ccattgcgga 1080
agattcccta ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggccg 1140
atcaccctct caggtcggct acgcatcgtt gccttggtga gccgttacct caccaactag 1200
ctaatgcgcc gcgggtccat ctgtaagtgg tagccgaagc caccttttat gtttgaacca 1260
tgcggttcaa acaaccatcc ggtattagcc ccggtttccc ggagttatcc cagtcttaca 1320
ggcaggttac ccacgtgtta ctcacccgtc cgccgctaac atcagggagc aagctcccat 1380
ctgtccgctc gac 1393
Claims (9)
1. one plant of salt tolerant produces the bacillus subtilis of 3-hydroxy-2-butanone, classification naming is bacillus subtilis (Bacillus
Subtilis), bacterial strain number is NRCB002, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Its deposit number is CGMCC NO.17213, and the deposit date is on January 18th, 2019.
2. the bacillus subtilis that salt tolerant according to claim 1 produces 3-hydroxy-2-butanone, which is characterized in that the withered grass gemma
Bacillus is thermophilic salt form bacillus subtilis, and in liquid medium, the tolerance of salinity reaches as high as 100g/L.
3. the bacillus subtilis that salt tolerant according to claim 1 produces 3-hydroxy-2-butanone, which is characterized in that the withered grass gemma
Bacillus, in the soil, the tolerance of salinity reach as high as 20g/kg.
4. salt tolerant described in claim 1 produces application of the bacillus subtilis of 3-hydroxy-2-butanone in fermenting and producing 3-hydroxy-2-butanone.
5. the bacillus subtilis that salt tolerant described in claim 1 produces 3-hydroxy-2-butanone is improving the application in the urgent ability of plant salt tolerance.
6. application according to claim 5, which is characterized in that the plant is clover.
7. application according to claim 5, which is characterized in that bacillus subtilis NRCB002 is applied to salt affected soil,
The salt affected soil concentration containing soluble-salt is up to 20g/kg.
8. the bacillus subtilis that salt tolerant described in claim 1 produces 3-hydroxy-2-butanone is promoting the application in plant growth.
9. application according to claim 8, which is characterized in that the plant is clover.
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