CN101665819A - Method for evaluating soil environment safely of transgenic drought-resistant soybeans - Google Patents
Method for evaluating soil environment safely of transgenic drought-resistant soybeans Download PDFInfo
- Publication number
- CN101665819A CN101665819A CN200910072986A CN200910072986A CN101665819A CN 101665819 A CN101665819 A CN 101665819A CN 200910072986 A CN200910072986 A CN 200910072986A CN 200910072986 A CN200910072986 A CN 200910072986A CN 101665819 A CN101665819 A CN 101665819A
- Authority
- CN
- China
- Prior art keywords
- transgenic
- root system
- soybean root
- drought
- resistant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a method for evaluating the soil environment safely of transgenic drought-resistant soybeans. The method comprises the following steps: respectively taking 100 g of transgenic drought-resistant soybean root system and 100 g of the same variety of non-transgenic soybean root system for homogenizing and filtering, centrifuging at low temperature by a refrigerated centrifuge 4,000 g, and extracting supernatant to be placed a refrigerator at 4 DEG C for storing and later use; respectively preparing a culture medium suitable for the growths of each functional microorganism and pathogenic microorganism, respectively adding 0, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the extract of the transgenic drought-resistant soybean root system and the same variety of non-transgenic soybean root system to each selective culture medium and then pouring plate, and repeating for five times; respectively inoculating the functional microorganism such as azotobacteria, silicate bacterium,trichoderma, bacillus megaterium and bacillus subtilis and the soybean root pathogenic bacteria such as phytophthora sojae in each different selective culture medium after pouring plate; after culturing for 5-7 days at 28-30 DEG C, measuring the diameters of the functional microorganisms and the pathogenic microorganisms in each plate culture medium, comparing the significances of differences ofthe diameters of each functional microorganism and each pathogenic microorganism in the culture medium containing the extracts of the transgenic drought-resistant soybean root system and the same variety of non-transgenic soybean root system, and determining whether the transgenic drought-resistant soybean has influence on the soil functional microorganism and the pathogenic microorganism or not.
Description
Affiliated technical field
The present invention relates to a kind of genetically engineered soybean environmental safety assessment method, especially method for evaluating soil environment safely of transgenic drought-resistant soybeans.
Background technology
Genetically engineered soybean is that goal gene is imported the new soybean varieties of formulating behind the soybean by certain method.Genetically engineered soybean is used in producing from formulating to, needs to have only the qualified genetically engineered soybean of safety evaluation to enter in the production and use through environment and biological safety evaluation.To the environmental safety assessment before the genetically modified crops commercialization be prevent the genetically modified crops establishing in large scale the important means that may bring to the ecosystem and human survival potential threat.China is at the early-stage aspect genetically engineered soybean new variety initiative research, and transgenic drought-resistant soybean edatope evaluation method is the edatope safe evaluation method of setting up in conjunction with the transgenic drought-resistant soybean of the independent intellectual property right of the present initiative of China.
Summary of the invention
Genetically engineered soybean is at present in the extensively plantation of European Union, the U.S., Canada, but at present the kind of plantation only is a transgenosis antiweed soybean, so the genetically engineered soybean environmental safety assessment method that country set up such as America and Europe also only is applicable to the environmental safety assessment to transgenosis antiweed soybean.The transgenic drought-resistant soybean is formulated for solving this restriction Chinese soybean main producing region yield and quality bottleneck problem of arid, because the goal gene that the transgenosis antiweed soybean of states such as transgenic drought-resistant soybean and America and Europe plantation at present imports is different, so the transgenosis antiweed soybean safe evaluation method of state's foundation such as America and Europe then is not suitable for the transgenic drought-resistant soybean of China's independent development is carried out the environmental safety evaluation.This invention proposes method for evaluating soil environment safely of transgenic drought-resistant soybeans, whether applying transgene drought resisting soybean edatope safe evaluation method can exert an influence to soil function microorganism, pathogenic micro-organism and soil enzyme after can detecting the transgenic drought-resistant soybean planting, to guaranteeing that this transgenic drought-resistant soybean that has a extensive future puts into production safely, the yield and quality that improves Chinese soybean production has vital role.In addition, this method also is applicable to the safety evaluation that other transgenic drought-resistant crops is carried out edatope.
This invention solves the technical scheme that its technical problem adopts: utilize transgenic drought-resistant soybean root system extract to add in the substratum, the soil function microorganism that separates from soil and identify and pathogenic micro-organism be inoculated in the above-mentioned substratum cultivate, observe and also measure of the influence of transgenic drought-resistant soybean root system secretory product soil function microorganism and pathogenic micro-organism growth and corresponding enzymic activity that functional microorganism produces thereof.
The invention has the beneficial effects as follows: in indoor employing substratum, add under the pure culture condition of transgenic drought-resistant soybean root system extract and suitable microorganism growth, can rapid detection transgenic drought-resistant soybean to the influence of soil function microorganism, pathogenic micro-organism growth and corresponding enzymic activity that functional microorganism produces.Indoor detection is easy to the condition of controling environment, accurate and sense cycle weak point.This method can be the transgenic drought-resistant crop from developing to the edatope evaluation method that puts into production and provide safe and reliable, guarantees that the transgenic drought-resistant crop safety puts into production, and obtains optimum economic benefit.
Embodiment
Get transgenic drought-resistant soybean root system and same kind non-transgenic soybean root system 100g homogenate after-filtration respectively, getting supernatant liquor under the cryogenic freezing whizzer 4000g after centrifugal, to place 4 ℃ of refrigerators to preserve standby.Prepare the selective medium that is fit to its growth respectively at every kind of functional microorganism and pathogenic micro-organism, in each selective medium, add above-mentioned transgenic drought-resistant soybean root system and same kind non-transgenic soybean root system extract 0 respectively, 1%, 0.1%, 0.01%, 0.001%, 0.0001% back is fallen dull and stereotyped, 5 repetitions.Inoculate respectively in the every kind of different selective medium that falls behind the flat board that functional microorganism such as vinelandii, silicate bacteria, wood are mould, bacillus megaterium, subtilis etc. and soybean root pathogenic bacteria such as phytophthora.Cultivate under 28 ℃～30 ℃ conditions and measure functional microorganism and pathogenic micro-organism colony diameter in each plate culture medium after 5～7 days, compare the significance of difference of each function yeast and pathogenic bacteria colony diameter in containing transgenic drought-resistant soybean and same kind non-transgenic soybean root system extract substratum, determine whether the transgenic drought-resistant soybean has influence to soil function microorganism and pathogenic micro-organism.
After continuing to be cultured to 10 days under 28～30 ℃ of conditions, dry back porphyrize under 28 ℃～30 ℃ conditions is measured the enzymic activity of function yeast correspondence in each training oxygen base, as urase, Phosphoric acid esterase and cellulase activity with the selective medium of above-mentioned each function yeast of inoculation.
Claims (1)
- Method for evaluating soil environment safely of transgenic drought-resistant soybeans is characterized in that:1. get transgenic drought-resistant soybean root system and same kind non-transgenic soybean root system 100g homogenate after-filtration respectively, getting supernatant liquor under the cryogenic freezing whizzer 4000g after centrifugal, to place 4 ℃ of refrigerators to preserve standby.Prepare the selective medium that is fit to its growth respectively at every kind of functional microorganism and pathogenic micro-organism, in each selective medium, add above-mentioned transgenic drought-resistant soybean root system and same kind non-transgenic soybean root system extract 0 respectively, 1%, 0.1%, 0.01%, 0.001%, 0.0001% back is fallen dull and stereotyped, 5 repetitions.Inoculate respectively in the every kind of different selective medium that falls behind the flat board that functional microorganism such as vinelandii, silicate bacteria, wood are mould, bacillus megaterium, subtilis etc. and soybean root pathogenic bacteria such as phytophthora.Cultivate under 28 ℃～30 ℃ conditions and measure functional microorganism and pathogenic micro-organism colony diameter in each plate culture medium after 5～7 days, compare the significance of difference of each function yeast and pathogenic bacteria colony diameter in containing transgenic drought-resistant soybean and same kind non-transgenic soybean root system extract substratum, determine whether the transgenic drought-resistant soybean has influence to soil function microorganism and pathogenic micro-organism.With the selective medium of above-mentioned each function yeast of inoculation after continuing to be cultured to 10 days under 28～30 ℃ of conditions, dry back porphyrize under 28 ℃～30 ℃ conditions, measure the enzymic activity of function yeast correspondence in each substratum, as urase, Phosphoric acid esterase and cellulase activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910072986A CN101665819A (en) | 2009-09-28 | 2009-09-28 | Method for evaluating soil environment safely of transgenic drought-resistant soybeans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910072986A CN101665819A (en) | 2009-09-28 | 2009-09-28 | Method for evaluating soil environment safely of transgenic drought-resistant soybeans |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101665819A true CN101665819A (en) | 2010-03-10 |
Family
ID=41802594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910072986A Pending CN101665819A (en) | 2009-09-28 | 2009-09-28 | Method for evaluating soil environment safely of transgenic drought-resistant soybeans |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101665819A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181388A (en) * | 2011-03-17 | 2011-09-14 | 重庆理工大学 | Method for separating bacteria producing cellulase |
| CN104025843A (en) * | 2014-05-21 | 2014-09-10 | 中国科学院东北地理与农业生态研究所 | Transgenic plant cultivation risk evaluation method based on soil fauna removal |
-
2009
- 2009-09-28 CN CN200910072986A patent/CN101665819A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181388A (en) * | 2011-03-17 | 2011-09-14 | 重庆理工大学 | Method for separating bacteria producing cellulase |
| CN104025843A (en) * | 2014-05-21 | 2014-09-10 | 中国科学院东北地理与农业生态研究所 | Transgenic plant cultivation risk evaluation method based on soil fauna removal |
| CN104025843B (en) * | 2014-05-21 | 2016-01-20 | 中国科学院东北地理与农业生态研究所 | Based on the genetically modified plants Planting risk evaluation method that soil fauna is removed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103740611A (en) | Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent | |
| CN109913523A (en) | A kind of culture medium of nitrogen source that screening suitable Bifidobacterium proliferation | |
| CN104694424B (en) | One plant of Bacillus amyloliquefaciens strain for being isolated from producing protease in fermented soya bean | |
| CN103013844A (en) | Aspergillus oryzae bacterial strain giving high yield of neutral protease and liquid fermentation method thereof | |
| CN103614447B (en) | A kind of Ferment of DM production method utilizing cane molasses Substitute For Partial W-Gum | |
| CN103205364B (en) | Aspergillus oryzae strain and application thereof to fructose-oligosaccharide fermentation production | |
| CN104313079B (en) | Preparation method of monensin premix | |
| CN103937691B (en) | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application | |
| Ni et al. | Identification and characterization of a new naringinase-producing strain, Williopsis californica Jmudeb007 | |
| CN103243037A (en) | High temperature yeast for brewing msalais, preparation method thereof and brewed msalais | |
| CN104805017A (en) | Plant endophytic fungus for generation of beta-glucosidase and application thereof | |
| CN103834606A (en) | Engineering strain expressing acid-resistant high-temperature alpha-amylase gene mutants | |
| CN101665819A (en) | Method for evaluating soil environment safely of transgenic drought-resistant soybeans | |
| Spinnler et al. | Pectinolytic activity of Clostridium thermocellum: its use for anaerobic fermentation of sugar beet pulp | |
| CN103343093B (en) | Low-temperature yeast for brewing musalaisi, preparation method and prepared musalaisi | |
| CN105543112B (en) | Aspergillus niger seed culture method | |
| CN102559843B (en) | Method for observing infection process of citrus canker pathogens in real time | |
| CN102181490B (en) | Method for producing citric acid | |
| CN106011004B (en) | Nitrogen-fixing microorganism G96, and preparation method and application of microbial inoculum thereof | |
| CN101712940B (en) | Klebsiella pneumonia strain and application thereof | |
| CN102676483A (en) | Method for producing protease through one-bacterium multi-enzyme strain | |
| CN110195081A (en) | Soybean protein solution zymotechnique | |
| CN102719513A (en) | Method for judging degrading capability of cellulose degradation bacteria | |
| CN105255842A (en) | Laccase for degrading lignin | |
| CN108865937A (en) | It is a kind of to utilize the raw sporiferous method of bacillus stearothermophilus mutant bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100310 |