CN103740611A - Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent - Google Patents
Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent Download PDFInfo
- Publication number
- CN103740611A CN103740611A CN201310703396.1A CN201310703396A CN103740611A CN 103740611 A CN103740611 A CN 103740611A CN 201310703396 A CN201310703396 A CN 201310703396A CN 103740611 A CN103740611 A CN 103740611A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- fermentation
- micro organism
- complex micro
- organism fungicide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a compound microorganism agent and application thereof, in particular relates to a compound microorganism agent, application of the compound microorganism agent in degradation of humic acid and an application method of the compound microorganism agent, aims at solving the problems of difficult degradation and low degradation rate of humic acid, provides the compound microorganism agent and application of the agent in the degradation of humic acid and provides a method for using the compound microorganism agent to degrade humic acid into micromolecules. The compound microorganism agent comprises bacillus, saccharomyces cerevisiae, lactobacillus and bacillus aceticus, wherein the bacillus comprises bacillus subtilis, bacillus licheniformis and bacillus pumilus; the lactobacillus comprises lactobacillus plantarum, lactobacillus acidophilus and streptococcus thermophilus. Due to the adoption of the agent, macromolecules of humic acid can be degraded into micromolecules.
Description
Technical field
The present invention relates to a kind of complex micro organism fungicide and application thereof, be specifically related to the application of a kind of complex micro organism fungicide and this microbial inoculum degraded humic acids and apply the method for this microbial inoculum degraded humic acids.
Background technology
In weathered coal, brown coal, peat, contain a large amount of humic acids (Ulmic acids, ulmic acid, xanthohumic acid), ordinary method is to extract with alkali lye, sulfuric acid precipitation, but macromolecular Ulmic acids, ulmic acid are water insoluble, and utilising efficiency is low, xanthohumic acid is easily utilized, but restricted levels.Different areas weathered coal, brown coal, three kinds of humic acids content and proportional difference are very large, so application is restricted.The humic acids complicated organism that to be animals and plants remains form through long-term physics, chemistry, biological action, manually obtains humic acids by animal and plant body through fermentative degradation, is also difficult to further this humic acids of degraded and obtains more micromolecular xanthohumic acid.Degraded humic acids is gone back the effective way of neither one at present.
Contriver finds under study for action, and some microorganism humic acids of can degrading becomes small molecules state, increases its solvability in water, and activity also increased, and more increased its range of application and effect.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, technical problem to be solved is humic acid degrading difficulty and the low problem of degradation rate, and the application of a kind of complex micro organism fungicide and this microbial inoculum degraded humic acids is provided, and provides this complex micro organism fungicide degraded humic acids of application to become micromolecular method.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of complex micro organism fungicide, comprise subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus lincheniforms), bacillus pumilus (Bacillus pumilus), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), plant lactobacillus (Lactobacillus plantarum), Lactobacterium acidophilum (Lactobacillus acidophilus), thermophilus streptococcus (Streptococcus thermophilus) and bacillus aceticus (Acetobacter).
The preferred weight ratio of described bacillus category, yeast saccharomyces cerevisiae, lactic acid bacteria class and bacillus aceticus is 4-6:0.5-1:2-3.5:0.5-1, wherein the weight ratio of three of bacillus category kinds of bacterial classification subtilises, Bacillus licheniformis and bacillus pumilus is 1:1:1, and the weight ratio of three kinds of bacterial classification plant lactobacilluss, Lactobacterium acidophilum and thermophilus streptococcuses of lactic acid bacteria class is 1:1:1.
The preferred weight ratio for the humic acids of degrading of described bacillus category, yeast saccharomyces cerevisiae, lactic acid bacteria class and bacillus aceticus is 5:1:3:1, wherein the weight ratio of three of bacillus category kinds of bacterial classification subtilises, Bacillus licheniformis and bacillus pumilus is 1:1:1, and the weight ratio of three kinds of bacterial classification plant lactobacilluss, Lactobacterium acidophilum and thermophilus streptococcuses of lactic acid bacteria class is 1:1:1.
Described complex micro organism fungicide can be applied to degraded humic acids.
The method of utilizing this complex micro organism fungicide degraded humic acids, comprises the following steps:
A, strain activation and culture: eight kinds of bacterial classifications are adopted to PDA substratum inoculation culture respectively, culture temperature 27-35 ℃, incubation time 24-36 hour, pH value nature; Wherein aerobic bacteria shake-flask culture, the standing cultivation of anerobe; Detect bacterium class viable count in each substratum and be greater than 10
9cfu/mL yeast viable count is greater than 10
7cfu/mL stops cultivating;
B, prepare humic acid fermentation substratum: 500 parts, 100 parts of the raw material humic acid materials of parts by weights meter, 20 parts of proteins, 10 parts of carbohydrates, 0.1 part, inorganic salt and water is prepared into substratum according to a conventional method, and substratum is carried out to sterilizing with steam, 121 ℃ of sterilizings 20 minutes, then displaced air pressurize is cooled to the suitableeest culture temperature to substratum simultaneously; Wherein protein is one or more composite in analysis for soybean powder, fish meal and peptone, and carbohydrate is one or more composite in glucose, molasses, sucrose, and inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride;
C, humic acid fermentation degraded: eight kinds of bacterial classifications after activation are mixed, obtain complex micro organism fungicide, described complex micro organism fungicide is inoculated in humic acid fermentation substratum, inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5, then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours, pH value finishes to obtain the humic acids product of degraded for 4.0-4.5 anaerobically fermenting.
The humic acid material that the present invention adopts is one or more in ammonium humate, Sodium salts humic acids, potassium humate, weathered coal, brown coal, peat.Wherein, for the fermentative degradation of weathered coal, brown coal, humic acid in peat, it is good being first translated into humate.
The humic acid fermentation liquid of gained of the present invention can dilute 500-1000 doubly as plant-growth regulator or fertilizer or fertilizer additive use.
Compared with prior art the present invention has following beneficial effect.
The invention provides a kind of complex micro organism fungicide, the current discovery of this microbial inoculum can, for the humic acids macromole of further degrading humate or can not directly be utilized by plant, make it be converted into the xanthohumic acid of small molecules solubility.
The invention provides a kind of complex microorganism degraded humic acids and become micromolecular method, the method production process is pollution-free; There is no byproduct; Technical process is simple; Improved the utilization ratio of resource; Expanded humic acids range of application.Degraded humic acids becomes small molecules, and two-step fermentation degraded humic acids is degraded fast many than solid fermentation, biological enzyme can be fully and Binding Capacity react.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.Experimental technique in following embodiment, if no special instructions, is ordinary method.
one, the screening of degraded Humic Acids By Microorganisms.
The minimum medium that is sole carbon source in order to Sodium salts humic acids carries out bacterial screening bacterial strain to be selected stroke-physiological saline solution is diluted, and on flat board, is coated with, and selects eugonic bacterium colony as primary election bacterial strain.The bacterial strain filtering out is carried out to degradation capability test, and substratum is the PDA substratum that adds 2% Sodium salts humic acids, cultivates 48 hours for 32 ℃, can see that periphery of bacterial colonies forms circular degraded circle, determines degradation capability according to degraded circle size.Hybrid bacterial strain carries out liquid shaking bottle cultivation, and 32 ℃ of culture temperature, after 48 hours, are carried out standing heat insulating culture, observes colour-change and is shoaled gradually to dark brown by black.From 25 bacillus of this laboratory preservation, filtered out the strong bacillus category bacterial strain of 3 strain capacity of decomposition, and done that 16rsDNA sequential analysis determines, yeast saccharomyces cerevisiae is separated from wine brewing Daqu, bacillus aceticus is gained from make vinegar fermentation song, and three kinds of milk-acid bacterias are standard production bacterial strains of buying.
two, the liquid of the humic acids after degraded flocculation extreme value is large.
In the standing process of sodium humate solution, it is serious that macromolecular substance produces flocculation phenomenon, has a strong impact on use, the degradation solution after microbiological deterioration, and flocculation extreme value increases, and solubility property is good, does not produce flocculation phenomenon.Examine under a microscope the reticulated structure that can see flocculation.
three, the mensuration of macromole humic acid degrading rate.
Gravimetric determination humic acid degrading rate for the present invention, also may have some organic or inorganic componentss in salt Acid precipitation, negligible.The method is first to use excessive NaOH (20%) that the Sodium salts humic acids in sample is all dissolved, centrifugal, collect supernatant, in supernatant solution, add saturated concentrated hydrochloric acid, humic acids produces precipitation, centrifugal again, supernatant discarded, then wash precipitation with water, discard water-soluble part, 105 ℃ precipitation is dried to constant weight, accurate weighing, calculates degradation rate.
Respectively with Datong District, Jincheng, be crushed to 60 orders from the weathered coal of stone, directly with complex micro organism fungicide degraded of the present invention, the same calculating degradation rate 4.6%, 6.3%, 3.2% of method, average 4.7%.May to be that in weathered coal, molecular size is variant cause the difference of degradation rate.Above weathered coal is become to fulvo acid salt with alkaline purification, then use microbiological deterioration, to the gravimetric determination of degradation solution humic acids, degradation rate reaches 18.7%, 21.3%, 16.5%, and average 18.8%.The degradation rate of humate is higher, and its reason is that biological enzyme fully contacts and reacts with humic acids molecular energy.
Embodiment 1
Subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, thermophilus streptococcus and eight kinds of bacterial classifications of bacillus aceticus are adopted to PDA substratum inoculation culture respectively, culture temperature is controlled at 27-35 ℃, incubation time 24-36 hour, pH value is nature; Wherein aerobic bacteria shake-flask culture, the standing cultivation of anerobe; Detect viable count in each substratum, bacterium class is greater than 10
9cfu/mL yeast is greater than 10
7cfu/mL stops cultivating;
By the bacterial classification after activation by weight, subtilis: Bacillus licheniformis: bacillus pumilus: yeast saccharomyces cerevisiae: plant lactobacillus: Lactobacterium acidophilum: thermophilus streptococcus: bacillus aceticus=2:2:2:1:1:1:1:1 mixes, and obtains complex micro organism fungicide.
Embodiment 2
Subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, thermophilus streptococcus and eight kinds of bacterial classifications of bacillus aceticus are adopted to PDA substratum inoculation culture respectively, culture temperature is controlled at 27-35 ℃, incubation time 24-36 hour, pH value is nature; Wherein aerobic bacteria shake-flask culture, the standing cultivation of anerobe; Detect viable count in each substratum, bacterium class is greater than 10
9cfu/mL yeast is greater than 10
7cfu/mL stops cultivating;
By the bacterial classification after activation by weight, subtilis: Bacillus licheniformis: bacillus pumilus: yeast saccharomyces cerevisiae: plant lactobacillus: Lactobacterium acidophilum: thermophilus streptococcus: bacillus aceticus=1.8:1.8:1.8:0.5:1.2:1.2:1.2:0.6 mixes, and obtains complex micro organism fungicide.
Embodiment 3
Subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, thermophilus streptococcus and eight kinds of bacterial classifications of bacillus aceticus are adopted to PDA substratum inoculation culture respectively, culture temperature is controlled at 27-35 ℃, incubation time 24-36 hour, pH value is nature; Wherein aerobic bacteria shake-flask culture, the standing cultivation of anerobe; Detect viable count in each substratum, bacterium class is greater than 10
9cfu/mL yeast is greater than 10
7cfu/mL stops cultivating;
By the bacterial classification after activation by weight, subtilis: Bacillus licheniformis: bacillus pumilus: yeast saccharomyces cerevisiae: plant lactobacillus: Lactobacterium acidophilum: thermophilus streptococcus: bacillus aceticus=1.4:1.4:1.4:0.8:0.7:0.7:0.7:0.3 mixes, and obtains complex micro organism fungicide.
Embodiment 4
Substratum is carried out to sterilizing with steam, 121 ℃ of sterilizings 20 minutes, then displaced air pressurize, substratum is cooled to the suitableeest culture temperature simultaneously, with the complex micro organism fungicide of embodiment 2, be inoculated in humic acid fermentation substratum, inoculation 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5, then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours, pH value finishes to obtain the humic acids product of degraded for 4.0-4.5 anaerobically fermenting.
Described humic acid fermentation substratum comprises the raw material of following weight fraction weight ratio: 100 parts of Sodium salts humic acidss, 20 parts of soyflours, 10 parts of glucose, 0.05 part of potassium primary phosphate, manganous sulfate 0.05,500 parts, water.
Embodiment 5
Substratum is carried out to sterilizing with steam, 121 ℃ of sterilizings 20 minutes, then displaced air pressurize, substratum is cooled to the suitableeest culture temperature simultaneously, with the complex micro organism fungicide of embodiment 1, be inoculated in humic acid fermentation substratum, inoculation 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5, then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours, pH value finishes to obtain the humic acids product of degraded for 4.0-4.5 anaerobically fermenting.
Described humic acid fermentation substratum comprises the raw material of following weight fraction weight ratio: 100 parts of Sodium salts humic acidss, 20 parts of fish meal, 10 parts, molasses, 0.03 part of potassium primary phosphate, manganous sulfate 0.03,0.04 part, calcium chloride, 500 parts, water.
Embodiment 6
Substratum is carried out to sterilizing with steam, 121 ℃ of sterilizings 20 minutes, then displaced air pressurize, substratum is cooled to the suitableeest culture temperature simultaneously, with the complex micro organism fungicide of embodiment 3, be inoculated in humic acid fermentation substratum, inoculation 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5, then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours, pH value finishes to obtain the humic acids product of degraded for 4.0-4.5 anaerobically fermenting.
Described humic acid fermentation substratum comprises the raw material of following weight fraction weight ratio: 100 parts of potassium humates, 20 parts of peptones, 10 parts of sucrose, 0.05 part of potassium primary phosphate, calcium chloride 0.05,500 parts, water.
Embodiment 7
The content of surveying ulmic acid, Ulmic acids in the Sodium salts humic acids of embodiment 4 by weighting method is 43%, and by volumetry, surveying xanthohumic acid content is 4%; Adopt the complex micro organism fungicide total humic acid content 9.5% in the humic acid fermentation liquid product obtaining that ferments, xanthohumic acid 2.9%, its degradation rate is 20%.Solvability is fine, is greater than the national standard of 8% liquid humic acids.
The present invention can summarize with other the specific form without prejudice to spirit of the present invention or principal character.Therefore, no matter from that, above-mentioned embodiment of the present invention all can only think explanation of the present invention can not limit invention, claims have been pointed out scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, any variation in implication and the scope suitable with claims of the present invention, all should think to be included in the scope of claims.
Claims (7)
1. a complex micro organism fungicide, it is characterized in that: comprise bacillus category, yeast saccharomyces cerevisiae, lactic acid bacteria class and bacillus aceticus, described bacillus category comprises subtilis, Bacillus licheniformis and bacillus pumilus, and described lactic acid bacteria class comprises plant lactobacillus, Lactobacterium acidophilum and thermophilus streptococcus.
2. a kind of complex micro organism fungicide according to claim 1, it is characterized in that: the weight ratio of described bacillus category, yeast saccharomyces cerevisiae, lactic acid bacteria class and bacillus aceticus is 4-6:0.5-1:2-3.5:0.5-1, wherein the weight ratio of three of bacillus category kinds of bacterial classification subtilises, Bacillus licheniformis and bacillus pumilus is 1:1:1, and the weight ratio of three kinds of bacterial classification plant lactobacilluss, Lactobacterium acidophilum and thermophilus streptococcuses of lactic acid bacteria class is 1:1:1.
3. a kind of complex micro organism fungicide according to claim 2, it is characterized in that: the weight ratio of described bacillus category, yeast saccharomyces cerevisiae, lactic acid bacteria class and bacillus aceticus is 5:1:3:1, wherein the weight ratio of three of bacillus category kinds of bacterial classification subtilises, Bacillus licheniformis and bacillus pumilus is 1:1:1, and the weight ratio of three kinds of bacterial classification plant lactobacilluss, Lactobacterium acidophilum and thermophilus streptococcuses of lactic acid bacteria class is 1:1:1.
4. according to a kind of complex micro organism fungicide described in claim 1 or 2 or 3, it is characterized in that: this composite fungus agent is applied to degraded humic acids.
5. the method for a kind of complex micro organism fungicide degraded humic acids according to claim 4, is characterized in that comprising the following steps:
(1) strain activation and culture: eight kinds of bacterial classifications are adopted to PDA substratum inoculation culture respectively, culture temperature 27-35 ℃, incubation time 24-36 hour, pH value is nature; Wherein aerobic bacteria shake-flask culture, the standing cultivation of anerobe; Detect viable count in each substratum, bacterium class is greater than 10
9cfu/mL, yeast is greater than 10
7cfu/mL stops cultivating;
(2) humic acid fermentation degraded: substratum is carried out to sterilizing with steam, 121 ℃ of sterilizings 20 minutes, then displaced air pressurize, substratum is cooled to the suitableeest culture temperature simultaneously, eight kinds of bacterial classifications after activation are mixed, obtain complex micro organism fungicide, described complex micro organism fungicide is inoculated in humic acid fermentation substratum, inoculation 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5, then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours, pH value finishes to obtain the humic acids product of degraded for 4.0-4.5 anaerobically fermenting,
Described humic acid fermentation substratum comprises the raw material of following weight fraction weight ratio: 100 parts of humic acid materials, 20 parts of proteins, 10 parts of carbohydrates, 0.1 part, inorganic salt, 500 parts, water; Wherein protein is one or more composite in analysis for soybean powder, fish meal and peptone, and carbohydrate is one or more composite in glucose, molasses, sucrose, and inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride.
6. the method for a kind of complex micro organism fungicide degraded humic acids according to claim 5, is characterized in that: described humic acid material is one or more in ammonium humate, Sodium salts humic acids, potassium humate, weathered coal, brown coal, peat.
7. the humic acid fermentation degradation products that prepared by the method for a kind of complex micro organism fungicide degraded humic acids according to claim 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310703396.1A CN103740611A (en) | 2013-12-19 | 2013-12-19 | Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310703396.1A CN103740611A (en) | 2013-12-19 | 2013-12-19 | Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103740611A true CN103740611A (en) | 2014-04-23 |
Family
ID=50497678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310703396.1A Pending CN103740611A (en) | 2013-12-19 | 2013-12-19 | Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103740611A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105085112A (en) * | 2015-08-18 | 2015-11-25 | 济南航晨生物科技有限公司 | Technique for activating lignite humic acid by using bioenzyme |
| CN105152809A (en) * | 2015-08-18 | 2015-12-16 | 济南航晨生物科技有限公司 | Technology for decomposing and activating humic acid of lignite by adopting compound microorganisms |
| CN105348013A (en) * | 2015-12-03 | 2016-02-24 | 梅庆波 | Preparation method of special humic acid bio-fertilizer for tea trees |
| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
| CN106544302A (en) * | 2016-12-06 | 2017-03-29 | 江西万茂科技有限公司 | A kind of complex micro organism fungicide for soil improvement and preparation method thereof |
| CN107518009A (en) * | 2017-07-14 | 2017-12-29 | 北京百丰天下生物科技有限公司 | Clear garden bacteria agent applied to germ cleaning and preparation method thereof |
| CN107573193A (en) * | 2017-09-27 | 2018-01-12 | 安徽金叶碳素科技有限公司 | A kind of microbial degradation lignite compounding liquid fertilizer and preparation method thereof |
| CN107815433A (en) * | 2017-12-08 | 2018-03-20 | 青岛益佰农肥业有限公司 | A kind of complex micro organism fungicide and its application in fermentation humic acid lignite produces fulvic acid |
| CN108558568A (en) * | 2018-05-04 | 2018-09-21 | 保山保农农业开发股份有限公司 | A kind of preparation method of the material containing humic acid organic fertilizer |
| CN109022333A (en) * | 2018-09-13 | 2018-12-18 | 中国科学院上海有机化学研究所湖州生物制造创新中心 | A kind of preparation method and applications of composite microbial fermentation bacteria agent |
| CN110079577A (en) * | 2019-05-06 | 2019-08-02 | 内蒙古柠植源生物科技有限公司 | The activation method of humic acid in a kind of weathered coal |
| CN110526752A (en) * | 2019-09-03 | 2019-12-03 | 西北农林科技大学 | A method of dry pail latrine being transformed using compound bacteria |
| CN110616246A (en) * | 2019-10-11 | 2019-12-27 | 北京大伟嘉生物技术股份有限公司 | Fermentation method for increasing content of sodium fulvate and microbial agent thereof |
| CN111575203A (en) * | 2020-05-18 | 2020-08-25 | 上海净业农业科技发展有限公司 | Agricultural compound microbial agent and preparation method thereof |
| CN114713615A (en) * | 2022-02-14 | 2022-07-08 | 生态环境部南京环境科学研究所 | Method for degrading polycyclic aromatic hydrocarbon in soil by activating persulfate through ferrous ions |
| CN116332375A (en) * | 2023-05-04 | 2023-06-27 | 深圳永清水务有限责任公司 | Biochemical treatment method of high-salt wastewater |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86105680A (en) * | 1986-07-26 | 1987-02-11 | 江西省樟树农业学校 | Produce method of humic acids-microbial protein feeding stuff and products thereof with weathered coal |
-
2013
- 2013-12-19 CN CN201310703396.1A patent/CN103740611A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86105680A (en) * | 1986-07-26 | 1987-02-11 | 江西省樟树农业学校 | Produce method of humic acids-microbial protein feeding stuff and products thereof with weathered coal |
Non-Patent Citations (3)
| Title |
|---|
| 张昕: "风化煤的微生物转化：I菌种筛选及转化能力测定（待续）", 《腐植酸》 * |
| 陈文求: "腐植酸的降解研究及进展", 《化工进展》 * |
| 韩云平: "腐殖酸高效降解菌的筛选研究", 《山西大学学报》 * |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105152809A (en) * | 2015-08-18 | 2015-12-16 | 济南航晨生物科技有限公司 | Technology for decomposing and activating humic acid of lignite by adopting compound microorganisms |
| CN105085112B (en) * | 2015-08-18 | 2018-05-01 | 济南航晨生物科技有限公司 | Utilize the technique of biological enzyme activation Lignitic Humic Acid |
| CN105152809B (en) * | 2015-08-18 | 2018-08-24 | 济南航晨生物科技有限公司 | The technique that complex microorganism decomposes activation Lignitic Humic Acid |
| CN105085112A (en) * | 2015-08-18 | 2015-11-25 | 济南航晨生物科技有限公司 | Technique for activating lignite humic acid by using bioenzyme |
| CN105348013A (en) * | 2015-12-03 | 2016-02-24 | 梅庆波 | Preparation method of special humic acid bio-fertilizer for tea trees |
| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
| CN106544302A (en) * | 2016-12-06 | 2017-03-29 | 江西万茂科技有限公司 | A kind of complex micro organism fungicide for soil improvement and preparation method thereof |
| CN107518009A (en) * | 2017-07-14 | 2017-12-29 | 北京百丰天下生物科技有限公司 | Clear garden bacteria agent applied to germ cleaning and preparation method thereof |
| CN107573193A (en) * | 2017-09-27 | 2018-01-12 | 安徽金叶碳素科技有限公司 | A kind of microbial degradation lignite compounding liquid fertilizer and preparation method thereof |
| CN107815433B (en) * | 2017-12-08 | 2021-03-12 | 青岛益佰农肥业有限公司 | Compound microbial agent and application thereof in producing fulvic acid by fermenting humic acid lignite |
| CN107815433A (en) * | 2017-12-08 | 2018-03-20 | 青岛益佰农肥业有限公司 | A kind of complex micro organism fungicide and its application in fermentation humic acid lignite produces fulvic acid |
| CN108558568A (en) * | 2018-05-04 | 2018-09-21 | 保山保农农业开发股份有限公司 | A kind of preparation method of the material containing humic acid organic fertilizer |
| CN109022333A (en) * | 2018-09-13 | 2018-12-18 | 中国科学院上海有机化学研究所湖州生物制造创新中心 | A kind of preparation method and applications of composite microbial fermentation bacteria agent |
| CN110079577A (en) * | 2019-05-06 | 2019-08-02 | 内蒙古柠植源生物科技有限公司 | The activation method of humic acid in a kind of weathered coal |
| CN110526752A (en) * | 2019-09-03 | 2019-12-03 | 西北农林科技大学 | A method of dry pail latrine being transformed using compound bacteria |
| CN110526752B (en) * | 2019-09-03 | 2022-03-18 | 西北农林科技大学 | Method for transforming dry latrine by using compound bacteria |
| CN110616246A (en) * | 2019-10-11 | 2019-12-27 | 北京大伟嘉生物技术股份有限公司 | Fermentation method for increasing content of sodium fulvate and microbial agent thereof |
| CN111575203A (en) * | 2020-05-18 | 2020-08-25 | 上海净业农业科技发展有限公司 | Agricultural compound microbial agent and preparation method thereof |
| CN114713615A (en) * | 2022-02-14 | 2022-07-08 | 生态环境部南京环境科学研究所 | Method for degrading polycyclic aromatic hydrocarbon in soil by activating persulfate through ferrous ions |
| CN116332375A (en) * | 2023-05-04 | 2023-06-27 | 深圳永清水务有限责任公司 | Biochemical treatment method of high-salt wastewater |
| CN116332375B (en) * | 2023-05-04 | 2024-02-23 | 深圳永清水务有限责任公司 | Biochemical treatment method of high-salt wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103740611A (en) | Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent | |
| US11898140B2 (en) | Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method | |
| AU2021103470A4 (en) | Bacillus subtilis bs40-4 strain and method for composting organic wastes by using the same | |
| CN110643544B (en) | Lactobacillus paracasei761, application thereof, silage additive and silage | |
| CN102199050A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN106278493B (en) | It is classified the method that enzymatic isolation method prepares the seaweed organic fertilizer containing oligosaccharides | |
| CN109439601A (en) | One plant of method for producing the bacterial strain of protease and its preparing alkali protease | |
| CN110241041A (en) | Compound microbial preparation, preparation method and application thereof | |
| CN102994430B (en) | Bacterial cellulose production strain and application thereof | |
| CN101885625B (en) | Solid fermentation method for producing microbial agent by using biomass power plant ash as carrier | |
| CN110055197B (en) | Paenibacillus amyloliquefaciens BREC-10 and microbial inoculum and application thereof | |
| CN103952331B (en) | One bacillus coagulans and the application in original position product separation fermenting lactic acid calcium thereof | |
| CN102334589B (en) | Method for preparing fermented feed from waste bacillus fermentation liquor | |
| CN102424626A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof | |
| CN102978142B (en) | Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin | |
| CN103289912A (en) | Solid fermentation method of bacillus coagulans | |
| CN103289911A (en) | Solid fermentation method for bacillus subtilis | |
| CN109810929A (en) | A method of utilizing the clay standby active microbial inoculum of cyanobacteria | |
| CN105219676A (en) | A kind of cultural method of bacillus laterosporus | |
| CN102352320B (en) | Novel myceliophthora thermophila strain and application thereof | |
| CN108821808A (en) | A kind of method and fertilizer preparing fertilizer using agricultural waste gurry | |
| CN112226380B (en) | Bacillus subtilis capable of degrading cellulose and application and preparation thereof | |
| CN106348884A (en) | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer | |
| CN106365709A (en) | Preparation method of seaweed organic-inorganic fertilizer containing bacillus megatherium fungicide | |
| CN105272406A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140423 |