CN101664102A - Method for preparing feed-grade whey powder substitute through bio-fermentation - Google Patents

Abstract

The invention provides a method for preparing a feed-grade whey powder substitute through bio-fermentation. The method comprises the following steps: endowing a product of the substitute with unique characteristics of biological products through a bioengineering technique by adopting a process of multi-strain multi-substrate mixed solid fermentation added with enzymes to ensure that various anti-nutritional factors in a fermentation substrate are reduced or eliminated; degrading starch substances into low-molecular-weight oligosaccharide or monosaccharide substances in favor of quick absorption of energy substances; performing enzymolysis the protein in the product by a complex protease to ensure that the protein exists in the form of peptide protein and the protein in the fermented product has excellent animal absorption ratio and bioavailability; retaining probiotics, the complex protease and active factors in an energy feed after fermentation to the maximum range through a reasonable and scientific production process; and considerably changing the mouthfeel of the product to ensure that the product has a sweet and sour taste liked by young stocks and is particularly favorable for increasing the food intake of piglets. As a sort of starchiness fermentation substrate selected by the energy feed, the feed-grade whey powder substitute makes full use of low-price energy feed rawmaterials in the present markets to reduce the product cost to the utmost extent on the premise of meeting the requirements on the energy nutrition for breeding the piglets and the like, and is favorable for the market development and the application popularization of the product.

Description

A kind of method with preparing feed-grade whey powder substitute through bio-fermentation

Affiliated technical field

The present invention relates to use biological method, adopt the technology of many bacterial classifications, many substrates, hybrid solid fermentation and external enzyme, is that substrate carries out the biofermentation modification to starchiness class energy feed, belongs to the feed fermentation field of engineering technology.

Background technology

Whey powder is a kind of auxilliary product that milk is produced cheese, is the good lactose source of sucking pig daily ration, and its good palatability, digestibility height, nutritive value are also higher, and effective energy value is higher than cerealses such as corn, is the rare energy feed raw material of a class.The main dependence on import of China, its produce market are mainly controlled by outer dish, and market fluctuation is big; Simultaneously because the whey powder price is high, fat-soluble avitaminosis, the salt content height, the component content variation is bigger, the substitute of whey powder is being sought one after another by enterprise both domestic and external, constantly comes into the market with relevant feed as the by-product of large quantities of dairy products such as acidifying powered milk substitute, MC.Whey powder development of substitutes technology is the focus of studying both at home and abroad at present, and the foreign study exploitation also enters the substantive production phase, but its technology all is in highly confidential state, and simultaneously a lot of technology and application are also carried out the tackling key problem stage.Therefore research, production, the use of China's whey powder substitute product have the important strategic meaning.

There are a large amount of ANFs problems in the byproduct as corn, wheat and agricultural product in the conventional energy feed, wherein mainly be non-starch mucopolysaccharide, protease inhibitor, phytolectin, phytic acid, pectin, antigen protein etc., these ANFs have reduced the digestive utilization ratio of feed to a certain extent.Starch, protein in special these primary raw materials all are macromolecular structures, and amino acid imbalance or the like in the single vegetalitas conventional energy feed has directly influenced the energy of feed, the normal production performance of the utilization rate of protein, especially weanling pig.For part solves the defective of conventional energy feed, be present main method by expanded, slaking and interpolation enzyme and probiotics preparation etc.Microorganism and Animal nutrition expert have done big quantity research and have used trial work from aspects such as piglet application supply systems both at home and abroad, wherein can reach bio-modification by microbial fermentation to the conventional energy feed, make the starchy material that is difficult to digestibility and utilization be degraded to materials such as low-molecular-weight oligosaccharides or monosaccharide, help absorbing of cubs such as piglet.

Summary of the invention

Present technique adopts the technology of many bacterial classifications, many substrates, hybrid solid fermentation and external enzyme to give the speciality that product has exclusive biological product by biotechnology.With corn be basic energy feed substrate raw material add wheat or and wheat bran and processing of farm products after byproduct, adding plant protein powder again is that auxiliary material ferments, multiple ANFs in the fermentation substrate is reduced and or eliminate; Make starchy material be degraded to low-molecular-weight oligosaccharides or monosaccharide material; The enzymolysis of protein in the product by protease exists with the state of peptide albumen, and protein material has splendid animal absorptivity and bioavilability; Probio, complex enzyme and active factors in the reservation fermentation back energy feed of the production technology maximum magnitude by reasonable science; The vegetable raw material of many substrates makes in the product amino acid structure balance that more becomes reasonable; On the feed mouthfeel after the fermentation sizable variation having taken place also, had the sour-sweet flavor that piglet is preferred, has helped increasing the food-intake of piglet.Kind aspect as the selected starchiness fermentation substrate of energy feed, make full use of the energy feed raw material that price is lower in the market, the maximum product cost that reduces helps the market development and the application of product under the prerequisite that full feeding piglet energy nutrient needs.

For above-mentioned purpose is achieved, the technical scheme that provides is as follows:

The present invention adopts the technology of many bacterial classifications, many substrates, hybrid solid fermentation and external enzyme, with corn is basic energy feed substrate raw material, add wheat or and wheat bran and processing of farm products after byproduct, add plant protein powder again and be auxiliary material and mix, inoculate, add technologies such as water mixing, fermentation, drying, pulverizing and finish product.

Among the above-mentioned preparation technology, fermentation substrate is a primary raw material with the corn of basic energy feed, add wheat or and wheat bran and processing of farm products after byproduct, adding plant protein powder again is auxiliary material.

In the above-mentioned manufacture craft, add above auxiliary material by the 0%-40% of primary raw material dry weight, and fully mix, wherein corn and wheat need break the pure graininess that becomes.

In the above-mentioned manufacture craft, (both ratios are mass ratio 8-6: 4-2) to add bacillus and yeast in the 1-3% of raw material dry weight; Add high activity zytase 100-150 unit, cellulase 100-300 unit by every gram raw material dry weight.

In the above-mentioned manufacture craft, raw material, fermentation strain and enzyme are mixed again, add entry gradually in mixed process, make mixed material moisture be controlled at 50 ± 5%, the best is 48%.

In the above-mentioned manufacture craft, mixed material is fermented, fermentation is carried out with the place in the open container of cleaning, and round includes but not limited to solid fermentation groove, solid-state fermentation tank and solid fermentation plate rail etc.

In the above-mentioned manufacture craft, the thickness of fermentation materials should not be higher than 70cm, and preferred heights is at 50-60cm.Fermentation temperature is at 20-55 ℃.The highlyest be no more than 55 ℃, when temperature is higher than 55 ℃, make suitable stirring, ventilate and handle suitable temperature adjustment and improve oxygen supply.

In the above-mentioned manufacture craft, fermentation time is 40-70 hour, and preferred fermentation time is 48 hours.

In the above-mentioned manufacture craft, drying process is to dry or low temperature drying equipment drying (temperature of charge is advisable less than 50 ℃) by natural air drying, the sun.

In the above-mentioned manufacture craft, dry back material is pulverized, and pulverizes more than 200 mesh sieves dry back material moisture≤12%.

It is as follows that the present invention and existing processes technology are compared advantage: 1, the speciality of the energy feed substrate being carried out bio-modification and giving the exclusive biological product of product with biological method.2, with corn, add wheat or and wheat bran and processing of farm products after byproduct be that price such as basic energy feed raw material is cheap, reduce product cost.3, the multiple ANFs in the fermentation substrate is reduced and or elimination.4, make starchy material be degraded to low-molecular-weight oligosaccharides or monosaccharide material, help the fast Absorption of energy matter.5, the enzymolysis of the protein in the product by protease exists with the state of peptide albumen, and protein material has splendid animal absorptivity and bioavilability.6, probio, complex enzyme and the active factors in the energy feed of the reservation of the production technology maximum magnitude by reasonable science fermentation back promotes digestion and the absorption of animal.7, it is reasonable that the vegetable raw material of many substrates makes in the product amino acid structure balance that more becomes, and helps Animal nutrition rationally and grow.8, on the feed mouthfeel after the fermentation sizable variation having taken place also, had the sour-sweet flavor that piglet is preferred, has helped increasing the food-intake of piglet.

The present invention is implemented by following concrete scheme, and described case study on implementation is not limited to the particular content of case.

Scheme 1: it is auxiliary material with soyabean protein powder 100kg that corn 800kg adds wheat 100kg, and wherein corn breaks with wheat and fully mixes after essence becomes graininess; Add bacillus and yeast (both ratios are mass ratio 5: 1) 20kg, add high activity zytase 2,000,000,000 units, cellulase 3,000,000,000 unit units; Raw material, fermentation strain and enzyme are added entry gradually mix again, make mixed material moisture be controlled at 50%; Mixed material is put into the fermentation of solid fermentation groove, and the thickness of fermentation materials is 50 hours at the 50cm fermentation time, and fermentation temperature is at 55 ℃, and suitable temperature adjustment and oxygen supply state are handled in suitable stirring, ventilation.After the fermentation ends less than 50 ℃ of drying equipment dryings; Crushed after being dried was pulverized more than 200 mesh sieves, and dry back moisture≤12% gets finished product.

Scheme 2: it is auxiliary material with plant protein powder 100kg that corn 800kg adds wheat 100kg, and wherein corn breaks with wheat and fully mixes after essence becomes graininess; Add bacillus and yeast (both ratios are mass ratio 5: 2) 15kg, add high activity zytase 1,500,000,000 units, cellulase 2,000,000,000 unit units; Raw material, fermentation strain and enzyme are added entry gradually mix again, make mixed material moisture be controlled at 52%; Mixed material is put into the solid-state fermentation tank fermentation, and the thickness of fermentation materials is 60 hours at the 55cm fermentation time, and fermentation temperature is at 55 ℃, and suitable temperature adjustment and oxygen supply state are handled in suitable stirring, ventilation.Under the sun, dry after the fermentation ends; Pulverize after the drying, pulverized more than 200 mesh sieves, dry back moisture≤12% gets finished product.

Scheme 3: it is auxiliary material with soyabean protein powder 1200kg that corn 8000kg adds wheat bran 800kg, and wherein corn breaks with wheat and fully mixes after essence becomes graininess; Add bacillus and yeast (both ratios are mass ratio 5: 1,5) 150kg, add high activity zytase 15,000,000,000 units, cellulase 20,000,000,000 unit units; Raw material, fermentation strain and enzyme are added entry gradually mix again, make mixed material moisture be controlled at 48%; Mixed bank is placed on the clean place that the sunlight direct projection is ventilated of keeping away, and the thickness of fermentation materials is 50 hours at the 60cm fermentation time, and fermentation temperature is at 55 ℃, and suitable temperature adjustment and oxygen supply state are handled in suitable stirring, ventilation.Carry out drying with temperature of charge less than 50 ℃ equipment after the fermentation ends; Pulverize after the drying, pulverized more than 200 mesh sieves, dry back moisture≤12% gets finished product.

Claims (10)

1, the present invention is basic energy feed raw material with corn, add wheat or and wheat bran and processing of farm products after byproduct, add plant protein powder again and be auxiliary material and mix, inoculate, add technologies such as water mixing, fermentation, drying, pulverizing and finish product.

2, among the above-mentioned preparation technology, fermentation substrate is a primary raw material with the corn of basic energy feed, add wheat or and wheat bran, processing of farm products after byproduct and plant protein powder be auxiliary material.

3, in the above-mentioned manufacture craft, add above auxiliary material by the 0%-40% of primary raw material dry weight, and fully mix, wherein corn and wheat need break the pure graininess that becomes.

4, in the above-mentioned manufacture craft, (both ratios are mass ratio 8-6: 4-2) to add bacillus and yeast in the 1-3% of raw material dry weight; Add high activity zytase 100-150 unit, cellulase 100-300 unit by every gram raw material dry weight.

5, in the above-mentioned manufacture craft, raw material, fermentation strain and enzyme are added entry more gradually mix, make mixed material moisture be controlled at 50 ± 5%, the best is 48%.

6, in the above-mentioned manufacture craft, mixed material is fermented, fermentation in the open container of cleaning or the place carry out, round includes but not limited to solid fermentation groove, solid-state fermentation tank and solid fermentation plate rail etc.

7, in the above-mentioned manufacture craft, the thickness of fermentation materials should not be higher than 70cm, and preferred heights is at 50-60cm.Fermentation temperature is at 20-55 ℃.The highlyest be no more than 55 ℃, when temperature is higher than 55 ℃, make suitable stirring, ventilate and handle suitable temperature adjustment and improve the oxygen supply state.

8, in the above-mentioned manufacture craft, fermentation time is 40-70 hour, and preferred fermentation time is 48 hours.

9, in the above-mentioned manufacture craft, drying process is to dry or low temperature drying equipment drying (temperature of charge is advisable less than 50 ℃) by natural air drying, the sun.

10, in the above-mentioned manufacture craft, dry back material is pulverized, pulverized more than 200 mesh sieves, dry back material moisture≤12% receive finished product.