CN101657113A - Use the preventing-killing method for injurious insects and the insect-pest exterminating device of carbon dioxide - Google Patents
Use the preventing-killing method for injurious insects and the insect-pest exterminating device of carbon dioxide Download PDFInfo
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Abstract
The invention provides a kind of preventing-killing method for injurious insects, it is characterized in that: the object that desinsection is handled is packed in the withstand voltage chamber, (i) be decompressed to-0.1013MPa～-reduced pressure treatment of 0.05MPa; (ii) then charge into carbon dioxide to the pressure below the above 1.0MPa of 0.6MPa and keep 15～90 minutes pressurized treatments of this pressurized state, (iii) then emitting carbon dioxide handles until the multiple pressure of normal pressure, then, carry out repeatedly more than 1 time above-mentioned (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple the pressure are handled.The present invention also provides a kind of insect-pest exterminating device, it is for passing through reduced pressure treatment, pressurized treatments and multiple temperature control of pressing in processing and the pressurized treatments, kill the device of the insect in object that survives, it is characterized in that possessing: be used to take in object withstand voltage chamber, be used to make the decompressing unit that is in negative pressure in this withstand voltage chamber, be used for filling arbon dioxide pressurizes in this withstand voltage chamber gas feed unit, be used for decompressing unit and be used for the temperature control unit that the temperature in the withstand voltage chamber is controlled to this from setting pressure to negative pressure.
Description
Technical field
The device that the present invention relates to use the preventing-killing method for injurious insects of carbon dioxide and be used for this method.
Background technology
Insecticidal technology about the preserved food products insect of using high-pressure carbon dioxide at length is recorded in “ Shi Grain, 44 " ((solely) Shi Pin Gross closes research institute) (" grain, 44 " (Independent Administrative Institution, Foodstuffs general Insitute)).
The initial example that uses the insecticidal technology of high-pressure carbon dioxide be the happy people of grade of light (non-patent literature 1) show with pressure 16kg/cm
2, 30 minutes processing times can drive away the infringement flour product tyrophagus putrescentiae (Tyrophagus putrescentiae).Afterwards, Germany had carried out research in this respect with France in 1985, and the Martin Bauer company of Germany has finished large-scale high-pressure carbon dioxide treating apparatus (patent documentation 1), and now, this device is used for the insect-pest exterminating of the medicinal plant (vanilla) of the said firm.Utilize the result of study of the present situation of the insecticidal technology that high-pressure carbon dioxide handles about Europe, people such as open country have carried out detailed report (non-patent literature 2).
On the other hand, in Japan, from the later stage eighties in last century, people such as middle north begin to study by oneself, verify the validity (patent documentation 2 etc.) of this technology to agricultural product and food.In addition, in Japan, people such as high bridge according to its to the person belonging to one of the lower social strata in the germination percentage of brown rice and the influence of taste of food (non-patent literature 3), palace according to insecticidal effect (non-patent literature 4) to the primary pest chestnut snipe curculionid (Curculio sikkimensis) of chestnut, estimated the validity of this technology.
The insecticidal mechanism of carbon dioxide is not illustrated as yet, but has following hypothesis.
(a) carbon dioxide acts on neural axis, destroys the balance of the Na/K ion that the conduction to nerve plays an important role.
(b) carbon dioxide makes the body fluid acidification of insect, suppresses various enzyme effects.
(c) act on and open and close the muscle that insect is used to absorb the hole that is called valve of oxygen, make valve be in open state, capture moisture in the body.
Use the insecticidal technology of high-pressure carbon dioxide to have following feature.
(a) thorough desinsection.
(b) processing time is short.
(c) toxicity of gas is low.
(d) noresidue.
(e) need special withstand voltage chamber and peripheral device.
As the conventional method of the insecticidal technology of existing use high-pressure carbon dioxide, can analogize following method (with reference to Fig. 2) according to patent documentation 2 and non-patent literature 2.
Damaged object is packed in the withstand voltage chamber, after following operation, take out object, place under certain condition.
(example) illustration is implemented pressure 3.0MPa, the treatment step in 50 minutes processing times (non-patent literature 2) to the ovum of lasioderma serricorne.
Step (1): carbon dioxide is boosted to 3.0MPa (0.1MPa/min) from normal pressure.
Step (2): under the pressure of 3.0MPa, kept 50 minutes.
Step (3): emit carbon dioxide and reduce to normal pressure (0.1MPa/min) by the pressure of 3.0MPa.(temperature in the chamber of step (2) is the temperature that nature forms, and is expected to be 15～25 ℃.)
According to people such as above-mentioned German Martin Bauer company and middle north, utilize the carbon dioxide of the pressure of 3.0MPa and 2.0MPa can realize the thorough desinsection of object.Therefore, (with regard to the pressure＞2.0MPa), present technique is established, and has the feature that processing time toxicity short, gas is low, do not have the residual problem with regard to thorough desinsection condition.
Yet the insect-killing device under the high pressure of 3.0MPa and 2.0MPa need comprise the special installation that can tolerate high pressure of autoclave.And, under the high pressure more than the 1MPa, must observe the regulation of gases at high pressure security personnel method, its use need be given the ratification to wait to use to go up and be added many conditions, is unsuitable for easy enforcement.
Patent documentation 1: No. 0458359 specification of European patent
Patent documentation 2: Japanese kokai publication hei 5-130854 communique
Non-patent literature 1: Shi Wei Chi, 14 (6), 511-516,1973
Non-patent literature 2: plant prevention of epidemis, 40,24,1995
Non-patent literature 3: Shi Gross grind Reported, and 65,33-37,2001
Non-patent literature 4: Guo real Japan, 58 (1), 40-43,2003
Summary of the invention
The present invention is conceived to utilize the advantage (problem that processing time toxicity short, gas is low, do not exist residual) of carbon dioxide desinsection, its purpose be to provide a kind of can be at the low pressure of pressure 2.0MPa (3.0MPa) than prior art, the technology of desinsection under the outer pressure condition of the gases at high pressure security personnel method scope of application particularly.
Main points of the present invention are as described below.
(1) a kind of preventing-killing method for injurious insects, it is characterized in that: the object that desinsection is handled is packed in the withstand voltage chamber, (i) be decompressed to-0.1013MPa～-reduced pressure treatment of 0.05MPa, (ii) then charge into carbon dioxide to the pressure below the above 1.5MPa of 0.6MPa and keep 15～90 minutes pressurized treatments of this pressurized state, (iii) then emitting carbon dioxide handles until the multiple pressure of normal pressure, then, carry out repeatedly more than 1 time above-mentioned (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple the pressure are handled.
(2) as above-mentioned (1) described preventing-killing method for injurious insects, it is characterized in that: the object that desinsection is handled is the agricultural product or the food of killed damaged by insect evil.
(3) as above-mentioned (1) or (2) described preventing-killing method for injurious insects, it is characterized in that: by charging into carbon dioxide, and kept this pressurized state 15～90 minutes, carry out pressurized treatments to more than the 0.6MPa and be lower than the pressure of 1MPa.
(4) as each described preventing-killing method for injurious insects in above-mentioned (1)～(3), it is characterized in that: withstand voltage chamber has the pressure-resistance structure of 0.6～1.7MPa.
(5) as each described preventing-killing method for injurious insects in above-mentioned (1)～(4), it is characterized in that: the temperature in the withstand voltage chamber during with pressurized treatments is set at 26～50 ℃.
(6) a kind of insect-pest exterminating device, it is by reduced pressure treatment, pressurized treatments and multiple temperature control of pressing in processing and the pressurized treatments, kill the insect that survives in object, it is characterized in that, possess: the withstand voltage chamber that is used to take in object, be used to make the decompressing unit that is in negative pressure in this withstand voltage chamber, be used for the gas feed unit that filling arbon dioxide pressurizes in this withstand voltage chamber, the temperature control unit that is used for decompressing unit and is used for the temperature in this withstand voltage chamber is controlled from setting pressure to negative pressure.
(7) as above-mentioned (6) described insect-pest exterminating device, it is characterized in that: withstand voltage chamber has the pressure-resistance structure of 0.6～1.7MPa.
(8) as above-mentioned (6) or (7) described insect-pest exterminating device, it is characterized in that: do not have the force (forcing) pump that the pressure that is used to make carbon dioxide raises.
(9) as each described insect-pest exterminating device in above-mentioned (6)～(8), it is characterized in that: outside the scope of application of gases at high pressure security personnel method.
The invention effect
According to the present invention, can utilize easy device to operate, and can reduce the carbon dioxide of use, not only can carry out the desinsection of crude drug safely, also can carry out the desinsection of far-ranging objects such as food safely.In addition, for the object of under the pressure of prior art, estimating to influence quality, also can realize not damaging the desinsection processing of quality.In addition,, the device (system) that is used to carry out desinsection can be simplified, the insect-killing device of vehicular (packaged type) can be made according to the present invention.
Description of drawings
Fig. 1 is the figure of an example of the treatment step of the treatment step of conventional method of the insecticidal technology of the existing use carbon dioxide of expression and the inventive method.The longitudinal axis is represented pressure (MPa), transverse axis represent the time (minute).
Fig. 2 is the figure of an example of expression insect-pest exterminating device of the present invention.
Symbol description
1,2,3,4,5,6: valve; A: pressure measuring unit; A2: control valve; B: pressure measuring unit; B2: control valve.
The specific embodiment
This specification comprises as the special 2007-85081 of hope of the Japanese publication of the application's basis for priority and the specification of the special 2008-57898 of hope of Japanese publication and/or the content that accompanying drawing is put down in writing.
The object of insect-pest exterminating of the present invention, so long as the article of killed damaged by insect evil, there is no particular limitation as to it, for example be agricultural product or food, be specifically as follows rice, wheat, cereals such as corn, soybean, beans such as red bean, fruit tree fruit classes such as chestnut, cassava, potato classes such as sweet potato, mushroom, dry vegetalbes such as drying stripped tuna, chrysanthemum, orchid, flowers such as rape, greengrocery, silk, fiber-like such as cotton, pepper, spices such as cloves, efficacy draft classes such as crude drug, wood type such as input timber etc., their processed goods (ground rice for example, wheat flour, tapioca starch, dessert, biscuit, macaroni, powder drink, paper bag etc.) and above-mentioned cereal, the seed of beans etc. etc.
The insect that the present invention is directed to is not particularly limited according to as the kind of the agricultural product of insect-pest exterminating object or food and different.
In preventing-killing method for injurious insects of the present invention, to slip into objects such as the agricultural product of insects such as maybe may slipping into harmful insect (ovum, larva, pupa, adult) or mite or food and pack in the withstand voltage chamber, (i) be decompressed to the reduced pressure treatment of specific negative pressure state; (ii) then carry out pressurized treatments, in this is handled, charge into carbon dioxide to the pressurized state gentler, and keep this pressurized state official hour than the pressure in the killing method of existing use high-pressure carbon dioxide; (iii) then carry out multiple pressure and handle, then, carry out above-mentioned more than 1 time (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple pressure processing repeatedly.Thus, can use, obtain basic and the same insecticidal effect of existing method than the simpler device of existing method.
The withstand voltage chamber that uses among the present invention gets final product so long as can tolerate the structure of the needed pressure of desinsection of the present invention, is not particularly limited.But, consider to tackle the interior pressure change that produces owing to variations in temperature from secure context, preferably have the withstand voltage chamber of the pressure-resistance structure of 0.6～1.7MPa, more preferably have the withstand voltage chamber of the pressure-resistance structure of 0.6～1.0MPa.
Gas concentration lwevel in improving withstand voltage chamber and promotion are dissolved in emitting and resorbent viewpoint of carbon dioxide the body fluid of harmful insect, the decompression degree in the above-mentioned reduced pressure treatment need for-0.1013MPa～-0.05MPa.This decompression degree is preferably-0.1MPa～-0.05MPa.The time of this reduced pressure treatment is different because of the scale of device, suitably selects to get final product.
Then, use CO 2 high pressure gas bomb etc., carbon dioxide is packed in the withstand voltage chamber that has been depressurized into the pressure below the above 1.5MPa of 0.6MPa.If this pressure is lower than 0.6MPa, then can not obtain effective insecticidal effect to the harmful insect (ovum, larva, pupa, adult, particularly ovum) of infringement object; If this pressure surpasses 1.0MPa, then device all needs special equipment everywhere.This pressurization degree is preferably and is lower than 1MPa, more preferably 0.9～0.99MPa more than the 0.6MPa.The rate of rise of this moment is generally 0.1～0.2MPa/min, is preferably 0.2MPa/min.
Among the present invention, after reaching the pressurized state of regulation, kept this pressurized state 15～90 minutes.If this retention time is lower than 15 minutes, then carbon dioxide is incomplete to the effect of harmful insect, the insecticidal effect that can not obtain to expect; If should the retention time surpass 90 minutes, then can not guarantee the validity of this technology.This retention time is preferably 20～60 minutes.
Temperature in the withstand voltage chamber during for pressurized treatments, the viewpoint of collateral security insecticidal effect is set out, and is preferably 26～50 ℃, more preferably 30～40 ℃.This temperature controlling can be undertaken by using heat-exchange device etc.
Then, carbon dioxide is handled by the multiple pressure that setting pressure returns to normal pressure, then, carried out once above above-mentioned (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple pressure processing repeatedly.When this is pressed again, can open withstand voltage chamber air is entered in the container, carry out reduced pressure treatment subsequently then, air is entered in the container, directly carry out reduced pressure treatment subsequently.
Preferably carry out 2 or 3 altogether and comprised above-mentioned (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple circulation of pressing the step of handling, more preferably carry out 2 circulations.
Insect-pest exterminating device of the present invention is by reduced pressure treatment, pressurized treatments and multiple temperature control of pressing in processing and the pressurized treatments, kill the insect that survives in object, it is characterized in that possessing: be used to take in object withstand voltage chamber, be used to make the decompressing unit that is in negative pressure in this withstand voltage chamber, be used for filling arbon dioxide pressurizes in this withstand voltage chamber gas feed unit, be used for decompressing unit and be used for the temperature control unit that the temperature in the withstand voltage chamber is controlled to this from setting pressure to negative pressure.
Because pressurized conditions of the present invention is gentler than the killing method of existing use high-pressure carbon dioxide, thus just enough from the pressure of CO 2 high pressure gas bomb, do not need force (forcing) pump.As decompressing unit, for example can use vavuum pump to reducing pressure in the withstand voltage chamber.Gas feed unit as filling arbon dioxide in withstand voltage chamber pressurizes for example can use CO 2 high pressure gas bomb and valve.As the temperature control unit that the temperature in the withstand voltage chamber is controlled, for example can use heat-exchange device.
Use Fig. 1 and Fig. 2, the structure and the method for operating of insect-pest exterminating device of the present invention is described.Fig. 2 represents to use the device of the equal pressing system of two grooves of two grooves (withstand voltage chamber), but the present invention is not limited to this device.Device shown in Figure 2 has the mechanism that supplies with heat from thermostat unit by heat-exchange device.
The desinsection object is packed among the withstand voltage chamber A, open valve 1, utilize vavuum pump to extract air in the container, shut off valve 1 (step (1)) out.At this moment, utilize pressure measuring unit a and control valve a2 control decompression rate. Open valve 2,3,4, send into carbon dioxide (step (2)) from the CO 2 high pressure gas bomb.At this moment, utilize pressure measuring unit b and the control valve b2 control rate of rise.Shut off valve 2,3,4 carries out the desinsection first time (step (3)).
Open valve 4,6, send into carbon dioxide from withstand voltage chamber A to the withstand voltage chamber B of decompression in advance, after the pressure balance, shut off valve 4,6 is opened valve 5, atmosphere opening.Shut off valve 5 is opened valve 1, utilizes vavuum pump to reduce pressure, shut off valve 1 (step (4)).At this moment, utilize pressure measuring unit a and control valve a2 control decompression rate. Open valve 2,3,4, send into carbon dioxide (step (5)) from the CO 2 high pressure gas bomb.At this moment, utilize pressure measuring unit b and the control valve b2 control rate of rise.Shut off valve 2,3,4 carries out the desinsection second time (step (6)).
Open valve 4,6, send into carbon dioxide from withstand voltage chamber A to the withstand voltage chamber B of decompression in advance, after the pressure balance, shut off valve 4,6 is opened valve 5, atmosphere opening (step (7)).
Take out the desinsection object from withstand voltage chamber A.
In above-mentioned steps (3) and step (6), utilize heat-exchange device to control in the withstand voltage chamber and the temperature of the carbon dioxide of sending into.
Embodiment
Below, by embodiment and comparative example the present invention is described in further detail, but the present invention is not limited thereto.
The effect of embodiment 1～18 and 1～8 pair of various crude drug of comparative example
(device)
Use insect-pest exterminating device shown in Figure 2.
(selection of test crude drug)
As the test crude drug, select genseng, Radix Angelicae Sinensis, rhizoma alismatis, date, peach kernel and polished rice.
About the insect of crude drug class and its infringement, following report is arranged:(i) people's (Sheng Yao magazine 1 (2): 31-38) (pharmacognosy magazine such as mulberry field, 1 (2): 31-38), people (tame room insects such as (ii) new fringe, the research To つ い て that 19 (2): 29 (1997) Bang Fang Wind The Bait To are learned with い タ バ コ シ バ Application system シ Sheng ) (house insect, 19 (2): 29 (1997), " for the ecological research of using radix glehniae as the lasioderma serricorne of bait "), the (iii) person belonging to one of the lower social strata in palace (Japanese ying the 51st time conference of Move thing entomology meeting, 2007, ノ シ メ マ ダ ラ メ イ ガ タ イ ソ ウ and the killed prediction To of び ト ウ ニ Application つ い て) (Japan uses the 51st conference of animal entomology meeting, 2007, " about the prediction of Indian meal moth infringement date and peach kernel ") .With reference to above-mentioned document, operation report has by the rhizoma alismatis of the example of Stegobium paniceum and lasioderma serricorne infringement, Radix Angelicae Sinensis, genseng as the test crude drug.In addition, people such as Yamamoto have reported lasioderma serricorne (the 18th bring back to life Yao Han Fang System drug microorganism and び Iso thing pollute countermeasure な ら ` び To Pin Quality management To Seki The Ru シ Application Port ジ ウ system) (the 18th time about the microorganism of crude drug traditional Chinese medical science preparation and the seminar of foreign substance pollution countermeasure and qualitative control) of can boring a hole to the deep of crude drug substance to these crude drugs, and prediction is handled very difficulty to the desinsection of these crude drugs.People etc. sneaks into as example with the foreign matter of Indian meal moth and has reported the infringement to fruits such as dry figs (ペ ス ト ロ ジ one, 2005) (pestology 2005) under the palace.Thus, with regard to crude drug, in order to predict as the date of the representative of fruit and seed and the infringement of peach kernel, as the test crude drug.
As the insect of cereals such as infringement rice and wheat, sitophilus zea-mais is widely known by the people, and with regard to crude drug, can predict its infringement to polished rice.
(explanation of crude drug)
Genseng is the root of the radicula of the genseng (Panax ginseng) of removing Araliaceae.Rhizoma alismatis is a stem tuber of removing stem, phyllopodium and the root of the rhizoma alismatis of Alismataceae (Alismataceae) or other kindred plant.Radix Angelicae Sinensis is the root of umbelliferous Radix Angelicae Sinensis or other kindred plant.Polished rice is grain of rice gramineous and the brown rice of having removed rice husk.Date is the fruit of rhamnaceous jujube or other kindred plants.Peach kernel is the seed of rosaceous peach.
(selection of test infringement worm)
As test infringement worm, select lasioderma serricorne, sitophilus zea-mais and Indian meal moth.About these infringement worms, steam northern ら サ イ エ Application ス Off オ one ラ system (" insect protected of farm imports and stifling handbook in the Ha Application De Block Star Network in cultural Choi insect dictionary independent administrative corporation culture Choi research institute (" insect dictionary " independent administrative corporation's cultural assets research institute) and Wheels Ru Farming product insect protected く ん, in work such as north, Science Forum publishing house publishes) in following record is arranged.
Sitophilus zea-mais after the Durchgangshohle inserts ovum on grain with long kiss and carries out reproduction in the hole.Larva after the hatching grows up in grain, becomes pupa.Pupa is sprouted wings, and dark brown adult gnaws to break in the seed coat and comes out from grain.As food, larva Durchgangshohle on food of the hatching of laying eggs on the surface becomes pupa to lasioderma serricorne with dry animals and plants matter, grows into behind the adult from food Durchgangshohle and comes out.According to these lasioderma serricornes and sitophilus zea-mais feature, can predict very difficulty of desinsection to the deep Durchgangshohle of object.
Known Indian meal moth is the preserved food products insect of numerous food products such as infringement brown rice, dry fruit and spice, as sneaking into foreign matter, is to sneak into the higher kind of frequency (Williams, 1964 Ann.Appl.Biol., 53,459-475 in the insect; Maillis, 1997, Handbook of PestControl).In addition, the larva that the person belonging to one of the lower social strata in palace has reported Indian meal moth therefrom comes out after Durchgangshohle is grown into adult in the peach kernel of crude drug and date, and (Japanese ying can the 51st time conference with Move thing entomology for this fact, 2007) (Japan uses the 51st conference of animal entomology meeting, 2007), therefore can predict very difficulty of desinsection.
(calculating of killing rate)
By the number of elements of the adult of the group of not using carbon dioxide to handle and processed group, calculate killing rate.
Killing rate (%)=[1-(becoming the one-tenth borer population of borer population/untreated fish group)] * 100
(mensuration of pressure and internal temperature)
To export recorder to from the signal of the sensor that is built in withstand voltage chamber, show measured value (MPa, ℃).
(carbon dioxide treatment group, open-assembly time 30 minutes)
30 lasioderma serricornes (adult) are put into the container that the 50g genseng is housed, kept 20 days, and after laying eggs, remove dead adult.The lasioderma serricorne (larva) of this moment and the container of genseng are put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 28 ℃ under pressure 0.95MPa.The container that this use carbon dioxide treatment is crossed kept 25 days at certain condition (30 ℃, humidity 70%, 16L8D), and 6 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that the 50g genseng is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 52 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 87%.
(carbon dioxide treatment group, open-assembly time 45 minutes)
Similarly to Example 1, the container of lasioderma serricorne (larva) and genseng is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 45 ℃ under pressure 0.95MPa.The container that this use carbon dioxide treatment is crossed kept 25 days at certain condition (30 ℃, humidity 70%, 16L8D), did not see the adult of lasioderma serricorne.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that the 50g genseng is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 52 lasioderma serricornes become adult in container.
(killing rate)
Killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 100%.
Comparative example 1
(carbon dioxide treatment group, 30 minutes open-assembly times, do not carry out reduced pressure treatment)
Similarly to Example 1, the container of lasioderma serricorne (larva) and genseng is put into withstand voltage chamber, charge into the pressure of carbon dioxide with 5 minutes, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Pressurize, keep and answer the operation of pressure once more repeatedly, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 28 ℃ under pressure 0.95MPa.The container that this use carbon dioxide treatment is crossed kept 25 days at certain condition (30 ℃, humidity 70%, 16L8D), and 26 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that the 50g genseng is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 52 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 51%.
Comparative example 2
(carbon dioxide treatment group, pressure 1.45MPa, 30 minutes open-assembly times, the number of occurrence 1 time)
Similarly to Example 1, the container of lasioderma serricorne (larva) and genseng is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 10 minutes then, kept 30 minutes, again with being depressed into normal pressure in 10 minutes again to 1.45MPa.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 28 ℃ under pressure 1.45MPa.The container that this use carbon dioxide treatment is crossed kept 25 days at certain condition (30 ℃, humidity 70%, 16L8D), and 15 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that the 50g genseng is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 52 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 70%.
(temperature is 19 ℃ in carbon dioxide treatment group, 45 minutes open-assembly times, the withstand voltage chamber)
30 lasioderma serricorne adults are put into the container that about 48g rhizoma alismatis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 19 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 9 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that about 48g rhizoma alismatis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 48 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 81%.
Embodiment 4
(temperature is 45 ℃ in carbon dioxide treatment group, 30 minutes open-assembly times, the withstand voltage chamber)
50 lasioderma serricorne adults are put into the container that about 45g rhizoma alismatis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 45 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days, not see the adult of lasioderma serricorne at certain condition (30 ℃, humidity 70%, 16L8D).
(carbon dioxide untreated fish group)
50 lasioderma serricorne adults are put into the container that about 45g rhizoma alismatis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 113 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide processed group repeatedly is 100%.
(22 ℃ of temperature, the number of occurrence 3 times in carbon dioxide processed group, 20 minutes open-assembly times, the withstand voltage chamber)
50 lasioderma serricorne adults are put into the container that about 45g rhizoma alismatis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 20 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Carry out repeatedly above-mentioned decompression (0.1MPa), pressurization (0.95MPa), the operation that keeps (20 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 20 minutes is 22 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 10 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
50 lasioderma serricorne adults are put into the container that about 45g rhizoma alismatis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 115 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide processed group repeatedly is 92%.
Comparative example 3
(carbon dioxide treatment group, 120 minutes open-assembly times, do not carry out 22 ℃ of temperature in the reduced pressure treatment, withstand voltage chamber, the number of occurrence 1 time)
Similarly to Example 3, the container of lasioderma serricorne (larva) and rhizoma alismatis is put into withstand voltage chamber, charge into the pressure of carbon dioxide with 5 minutes, kept 120 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.At this moment, the final temperature in the withstand voltage chamber of maintenance after 120 minutes is 22 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 46 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
50 lasioderma serricorne adults are put into the container that about 45g rhizoma alismatis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 113 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 61%.
Embodiment 6
(temperature is 19 ℃ in carbon dioxide treatment group, 45 minutes open-assembly times, the withstand voltage chamber)
30 lasioderma serricorne adults are put into the container that about 34g Radix Angelicae Sinensis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Carry out repeatedly once more above-mentioned decompression (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 19 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 80 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that about 34g Radix Angelicae Sinensis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 229 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide processed group repeatedly is 65%.
Embodiment 7
(temperature is 44 ℃ in carbon dioxide treatment group, 30 minutes open-assembly times, the withstand voltage chamber)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 44 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 2 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 272 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 99%.
(22 ℃ of temperature, the number of occurrence 3 times in carbon dioxide treatment group, 20 minutes open-assembly times, the withstand voltage chamber)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 20 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Carry out repeatedly 3 above-mentioned decompressions (0.1MPa), pressurization (0.95MPa), the operation that keeps (20 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 20 minutes is 22 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 25 days at certain condition (30 ℃, humidity 70%, 16L8D), 62 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 272 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 77%.
Comparative example 4
(carbon dioxide treatment group, 120 minutes open-assembly times, do not carry out 22 ℃ of temperature in the reduced pressure treatment, withstand voltage chamber, the number of occurrence 1 time)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and after laying eggs, remove dead adult.This container is put into withstand voltage chamber, charge into the pressure of carbon dioxide with 5 minutes, kept 120 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.At this moment, the final temperature in the withstand voltage chamber of maintenance after 120 minutes is 22 ℃ under pressure 0.95MPa.Container was kept 25 days at certain condition (30 ℃, humidity 70%, 16L8D), and 124 lasioderma serricornes become adult.
(carbon dioxide untreated fish group)
30 lasioderma serricorne adults are put into the container that about 35g Radix Angelicae Sinensis is housed, kept 20 days, and remove dead adult after laying eggs, kept 25 days again, 272 lasioderma serricornes become adult in container.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 54%.
The treatment conditions and the result of embodiment 1～8 and comparative example 1～4 are shown in table 1.
[table 1]
Comparative example 5
(carbon dioxide treatment, pressure 0.5MPa, 120 minutes open-assembly times, do not carry out 17 ℃ of temperature in the reduced pressure treatment, withstand voltage chamber, the number of occurrence 1 time)
The container that 100 corundum Sitophilusspp. imagos are housed is put into withstand voltage chamber, charge into the pressure of carbon dioxide with 3 minutes, keep after 120 minutes, again with being depressed into normal pressure in 5 minutes again to 0.5MPa.In this container, add the 30g polished rice reposefully, placed 6 days.97 sitophilus zea-maises have been reclaimed from this container with locomotor activity.Average killing rate when carrying out the experiment of 5 deuterzooid repeatedly is 3%.
Comparative example 6
(17 ℃ of temperature, the number of occurrence 2 times in carbon dioxide treatment, pressure 0.5MPa, 30 minutes open-assembly times, the withstand voltage chamber)
The container that 200 corundum Sitophilusspp. imagos are housed is put into withstand voltage chamber, be decompressed to-0.1MPa after, charge into the pressure of carbon dioxide with 5 minutes to 0.5MPa, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.5MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 17 ℃ under pressure 0.5MPa.The container that this use carbon dioxide treatment is crossed kept 3 hours at certain condition (30 ℃, humidity 70%, 16L8D), and the sitophilus zea-mais with locomotor activity is 190.The killing rate of this experiment is 5%.
Comparative example 7
(carbon dioxide treatment, pressure 0.95MPa, 60 minutes open-assembly times, do not carry out 23 ℃ of temperature in the reduced pressure treatment, withstand voltage chamber, the number of occurrence 1 time)
The container that 100 corundum Sitophilusspp. imagos are housed is put into withstand voltage chamber, charge into carbon dioxide by the pressure of normal pressure with 5 minutes, kept 60 minutes, then with being depressed into normal pressure in 5 minutes again to 0.95MPa.In the container that this use carbon dioxide treatment is crossed, add the 30g polished rice reposefully, placed 6 days.24 sitophilus zea-maises have been reclaimed from this container with locomotor activity.The killing rate of this experiment is 76%.
Embodiment 9
(temperature is 19 ℃ in carbon dioxide treatment, 30 minutes open-assembly times, the withstand voltage chamber)
The container that 200 corundum Sitophilusspp. imagos are housed is put into withstand voltage chamber, be decompressed to-0.1MPa after, charge into the pressure of carbon dioxide with 5 minutes to 0.95MPa, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 19 ℃ under pressure 0.95MPa.In the container that this use carbon dioxide treatment is crossed, add the 30g polished rice reposefully, placed 4 days.10 sitophilus zea-maises have been reclaimed from this container with locomotor activity.The killing rate of this experiment is 95%.
Embodiment 10
(temperature is 23 ℃ in carbon dioxide treatment group, 30 minutes open-assembly times, the withstand voltage chamber)
The adult (about 50) of sitophilus zea-mais is put into the container that about 20g polished rice is housed, kept 3 days, and after laying eggs, remove adult.Further this container was kept 8 days down at certain condition (30 ℃ of temperature, humidity 70%, 16L8D), put into withstand voltage chamber then, be decompressed to-0.1MPa, then with charging into the pressure of carbon dioxide in 5 minutes to 0.95MPa, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 23 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 35 days down at certain condition (30 ℃, humidity 70%, 16L8D), 1 sitophilus zea-mais becomes adult.
(carbon dioxide untreated fish group)
106 sitophilus zea-maises become adult operating equally in the container of untreated fish group made from the carbon dioxide treatment group.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 99%.
Embodiment 11
(temperature is 17 ℃ in carbon dioxide treatment group, 60 minutes open-assembly times, the withstand voltage chamber)
The adult (about 50) of sitophilus zea-mais is put into the container that about 20g polished rice is housed, kept 3 days, and after laying eggs, remove adult.Further this container was kept 8 days down at certain condition (30 ℃ of temperature, humidity 70%, 16L8D), put into withstand voltage chamber then, be decompressed to-0.1MPa, then with charging into the pressure of carbon dioxide in 5 minutes to 0.95MPa, kept 60 minutes, again with being depressed into normal pressure in 5 minutes again.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (60 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 60 minutes is 17 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 35 days down, not see the adult of sitophilus zea-mais at certain condition (30 ℃, humidity 70%, 16L8D).
(carbon dioxide untreated fish group)
106 sitophilus zea-maises become adult operating equally in the container of untreated fish group made from the carbon dioxide treatment group.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 100%.
Embodiment 12～16 and comparative example 8
Sitophilus zea-mais (worm's ovum)
Embodiment 12
(temperature is 22 ℃ in carbon dioxide treatment group, 30 minutes open-assembly times, the withstand voltage chamber)
The adult (about 50) of sitophilus zea-mais is put into the container that about 20g polished rice is housed, kept 3 days, and after laying eggs, remove adult.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 22 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down at certain condition (30 ℃, humidity 70%, 16L8D), 34 sitophilus zea-maises become adult.
(carbon dioxide untreated fish group)
88 sitophilus zea-maises become adult in the container of operating the untreated fish group of making similarly to Example 12.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 62%.
Embodiment 13
(temperature is 43 ℃ in carbon dioxide treatment group, 30 minutes open-assembly times, the withstand voltage chamber)
Similarly to Example 12, the container of sitophilus zea-mais (ovum) and polished rice is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 30 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 43 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down, not see the adult of sitophilus zea-mais at certain condition (30 ℃, humidity 70%, 16L8D).
(carbon dioxide untreated fish group)
117 sitophilus zea-maises become adult in the container of operating the untreated fish group of making similarly to Example 13.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 100%.
Embodiment 14
(temperature is 22 ℃ in carbon dioxide treatment group, 45 minutes open-assembly times, the withstand voltage chamber)
Similarly to Example 12, the container of sitophilus zea-mais (ovum) and polished rice is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 22 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down at certain condition (30 ℃, humidity 70%, 16L8D), 10 sitophilus zea-maises become adult.
(carbon dioxide untreated fish group)
88 sitophilus zea-maises become adult in the container of operating the untreated fish group of making similarly to Example 14.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 88%.
Embodiment 15
(25 ℃ of temperature, the number of occurrence 3 times in carbon dioxide treatment group, 20 minutes open-assembly times, the withstand voltage chamber)
Similarly to Example 12, the container of sitophilus zea-mais (ovum) and polished rice is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 20 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Carry out repeatedly 3 decompressions (0.1MPa), pressurization (0.95MPa), the operation that keeps (20 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 20 minutes is 25 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down at certain condition (30 ℃, humidity 70%, 16L8D), 18 sitophilus zea-maises become adult.
(carbon dioxide untreated fish group)
71 sitophilus zea-maises become adult in the container of operating the untreated fish group of making similarly to Example 15.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 75%.
Comparative example 8
(temperature is 24 ℃ in carbon dioxide treatment group, 90 minutes open-assembly times, the withstand voltage chamber)
Similarly to Example 12, the container of sitophilus zea-mais (ovum) and polished rice is put into withstand voltage chamber, charge into the pressure of carbon dioxide with 5 minutes, kept 90 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.At this moment, the final temperature in the withstand voltage chamber of maintenance after 90 minutes is 24 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down at certain condition (30 ℃, humidity 70%, 16L8D), 27 sitophilus zea-maises become adult.
(carbon dioxide untreated fish group)
71 sitophilus zea-maises become adult in the container of the untreated fish group made from comparative example 8 same operations.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 62%.
Embodiment 16
(temperature is 22 ℃ in carbon dioxide treatment group, pressure 1.45MPa, 30 minutes open-assembly times, the withstand voltage chamber)
Similarly to Example 12, the container of sitophilus zea-mais (ovum) and polished rice is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 10 minutes then, kept 30 minutes, again with being depressed into normal pressure in 7 minutes again to 1.45MPa.And reduce pressure repeatedly (0.1MPa), pressurization (1.45MPa), the operation that keeps (30 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 30 minutes is 22 ℃ under pressure 1.45MPa.Further will use container that carbon dioxide treatment crosses to keep 42 days down at certain condition (30 ℃, humidity 70%, 16L8D), 9 sitophilus zea-maises become adult.
(carbon dioxide untreated fish group)
88 sitophilus zea-maises become adult in the container of operating the untreated fish group of making similarly to Example 16.
(killing rate)
Average killing rate when carrying out the experiment of 5 carbon dioxide treatment groups repeatedly is 90%.
Embodiment 17～18
Indian meal moth (larva)
Embodiment 17
(temperature is 34 ℃ in carbon dioxide treatment group, 45 minutes open-assembly times, the withstand voltage chamber)
A large amount of Indian meal moth (mixing of larva and ovum) is put into the container that about 60g date is housed, kept under certain condition 14 days.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 34 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 45 days down, not see the adult of Indian meal moth at certain condition (30 ℃, humidity 70%, 16L8D).
(carbon dioxide untreated fish group)
24 Indian meal moths become adult in the container of operating the untreated fish group of making similarly to Example 17.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 100%.
Embodiment 18
(carbon dioxide treatment group, temperature is 34 ℃ in 45 minutes open-assembly times, the withstand voltage chamber)
A large amount of Indian meal moth (mixing of larva and ovum) is put into the container that about 50g peach kernel is housed, kept under certain condition 14 days.This container is put into withstand voltage chamber, be decompressed to-0.1MPa, charged into the pressure of carbon dioxide with 5 minutes then, kept 45 minutes, again with being depressed into normal pressure in 5 minutes again to 0.95MPa.Reduce pressure repeatedly once more (0.1MPa), pressurization (0.95MPa), the operation that keeps (45 minutes) and multiple pressure, under normal pressure, take out container.At this moment, the final temperature in the withstand voltage chamber of maintenance after 45 minutes is 34 ℃ under pressure 0.95MPa.Further will use container that carbon dioxide treatment crosses to keep 45 days down, not see the adult of Indian meal moth at certain condition (30 ℃, humidity 70%, 16L8D).
(carbon dioxide untreated fish group)
23 Indian meal moths become adult in the container of operating the untreated fish group of making similarly to Example 18.
(killing rate)
Average killing rate when carrying out the experiment of 3 carbon dioxide treatment groups repeatedly is 100%.
The treatment conditions and the result of embodiment 9～16 and comparative example 5～8 are shown in table 2.Treatment conditions and the result of embodiment 17～18 are shown in table 3.
[table 2]
[table 3]
The effect of embodiment 19～21 and 9 pairs of chestnuts of comparative example
(test objective)
Affirmation is in using the desinsection of carbon dioxide, with pressure the present invention of being lower than 1MPa insecticidal effect to chestnut.
(sample)
Use following two kinds of chestnuts, these two kinds of chestnuts are not all carried out the stifling processing of bromomethane.
(1) the Miyazaki county produces, and kind " is built ripple "
(2) the Ibaraki county produces, and kind " お く In " is set control group respectively with mixing of other kind.
(device)
Use insect-pest exterminating device shown in Figure 2.
(mensuration purpose)
Temperature Influence when (1) handling
30 ℃ (sample is preheated to 30 ℃) and 20 ℃
(2) research of cycle of treatment number of times
Number of processes (circulation) 1 time and 2 times
(evaluation)
After the processing, chestnut is remained on 25 ℃, compare the sum of the larva number of elements that from chestnut, comes out in control group and the processed group, evaluation effect.
Killing rate=[1-(summation of number appears in the larva of processed group) ÷ (the number summation appears in the larva of control group)] * 100
(treatment conditions)
Study condition setting as shown in table 4.1 group for the treatment of capacity of 1 time is set at 8kg, " building ripple " that the Miyazaki county produces carried out 3 same experiments repeatedly, " お く In " that the Ibaraki county is produced carries out 2 same experiments repeatedly with mixing of other kind, calculates the summation that number appears in larva.
(preparation of sample)
(1) The pre-heat treatment group: sample takes out from frozen state, at room temperature place more than 1 day, put it into then and carry out The pre-heat treatment in the insulating box, roughly reach 30 ℃ (desinsection was handled preceding 4 hours, inserted the probe assay of temperature measuring device) until the temperature of chestnut inside center portion.
(2) other processed group: sample takes out from frozen state, at room temperature places more than 1 day.
(basic processing method)
Carry out the circulation of 2 times following (1)～(4) repeatedly.But, in comparative example 9, only carry out the circulation of 1 time (1)～(4).
(1) is decompressed to about 10～4mmHg (0.1MPa) with vavuum pump.
(2) send into carbon dioxide to 0.98MPa.
(3) kept 30 minutes.
(3) with being depressed into normal pressure (0.2MPa/min) in about 5 minutes again.
(result)
Observed the Ibaraki at the 36th day and produce group, after 35 days, observe Miyazaki and produce group.Though parasitic rate there are differences because of the place of production of chestnut,, can observe the validity of carbon dioxide treatment of the present invention significantly to chestnut snipe curculionid.
[table 4]
To chestnut snipe curculionid, produce in the sample in the Ibaraki, handling killing rate by 1 time is 96.6%, handling killing rate by 2 times is 99.4%; Produce in the sample in Miyazaki, handle by 2 times, be 100% at the killing rate of 20 ℃ and 30 ℃.Therefore, also be more than 95% even carry out handling killing rate 1 time, but the better effects if of handling for 2 times.In addition, about The pre-heat treatment, all can observe enough insecticidal effects when carrying out The pre-heat treatment and when not carrying out The pre-heat treatment.
The content of all publications, patent and the patent application of quoting in this specification is directly quoted in patent specification as a reference.
Industrial utilizability
The present invention can be as the insect-pest exterminating technology in agricultural product or the food.
Claims (9)
1. preventing-killing method for injurious insects is characterized in that:
The object that desinsection is handled is packed in the withstand voltage chamber, (i) be decompressed to-0.1013MPa～-reduced pressure treatment of 0.05MPa, (ii) then charge into carbon dioxide to the pressure below the above 1.5MPa of 0.6MPa and keep 15～90 minutes pressurized treatments of this pressurized state, (iii) then emitting carbon dioxide handles until the multiple pressure of normal pressure
Then, carry out repeatedly more than 1 time described (i) reduced pressure treatment, (ii) pressurized treatments and (iii) multiple the pressure are handled.
2. preventing-killing method for injurious insects as claimed in claim 1 is characterized in that:
The object that desinsection is handled is the agricultural product or the food of killed damaged by insect evil.
3. preventing-killing method for injurious insects as claimed in claim 1 or 2 is characterized in that:
By charging into carbon dioxide, and kept this pressurized state 15～90 minutes, carry out pressurized treatments to more than the 0.6MPa and be lower than the pressure of 1MPa.
4. as each described preventing-killing method for injurious insects in the claim 1～3, it is characterized in that:
Withstand voltage chamber has the pressure-resistance structure of 0.6～1.7MPa.
5. as each described preventing-killing method for injurious insects in the claim 1～4, it is characterized in that:
Temperature in the withstand voltage chamber during with pressurized treatments is set at 26～50 ℃.
6. insect-pest exterminating device, its by reduced pressure treatment, pressurized treatments and multiple press handle and pressurized treatments in temperature control, kill the insect that survives in object, it is characterized in that possessing:
Be used to take in the withstand voltage chamber of object, be used to make the decompressing unit that is in negative pressure in this withstand voltage chamber, be used for the gas feed unit that filling arbon dioxide pressurizes in this withstand voltage chamber, the temperature control unit that is used for decompressing unit and is used for the temperature in this withstand voltage chamber is controlled from setting pressure to negative pressure.
7. insect-pest exterminating device as claimed in claim 6 is characterized in that:
Withstand voltage chamber has the pressure-resistance structure of 0.6～1.7MPa.
8. as claim 6 or 7 described insect-pest exterminating devices, it is characterized in that:
Do not have the force (forcing) pump that the pressure that is used to make carbon dioxide raises.
9. as each described insect-pest exterminating device in the claim 6～8, it is characterized in that:
Outside the scope of application of gases at high pressure security personnel method.
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| JP085081/2007 | 2007-03-28 | ||
| JP2008057898A JP5322045B2 (en) | 2007-03-28 | 2008-03-07 | Pest control method and pest control apparatus using carbon dioxide |
| JP057898/2008 | 2008-03-07 | ||
| PCT/JP2008/054589 WO2008117672A1 (en) | 2007-03-28 | 2008-03-13 | Method of insect pests control and insect pests control apparatus using carbon dioxide |
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| JP6194212B2 (en) * | 2013-09-17 | 2017-09-06 | 国立大学法人宇都宮大学 | How to kill insects |
| KR101877220B1 (en) * | 2018-01-19 | 2018-07-12 | 주식회사 컬프 | Pest control method of poultry farm using carbon dioxide |
| JP7262151B1 (en) | 2022-05-23 | 2023-04-21 | 東化研株式会社 | Fumigation method |
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| JPS5587723A (en) * | 1978-12-27 | 1980-07-02 | Tsumura Juntendo Inc | Preservation of crude drug |
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| CN101657113B (en) | 2013-10-16 |
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