CN101650288B - Method for determining content of natural rubber in rubber seed oil - Google Patents
Method for determining content of natural rubber in rubber seed oil Download PDFInfo
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- CN101650288B CN101650288B CN2008101310546A CN200810131054A CN101650288B CN 101650288 B CN101650288 B CN 101650288B CN 2008101310546 A CN2008101310546 A CN 2008101310546A CN 200810131054 A CN200810131054 A CN 200810131054A CN 101650288 B CN101650288 B CN 101650288B
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- 229920000263 Rubber seed oil Polymers 0.000 title claims abstract description 26
- 244000043261 Hevea brasiliensis Species 0.000 title claims abstract description 20
- 229920003052 natural elastomer Polymers 0.000 title claims abstract description 20
- 229920001194 natural rubber Polymers 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 19
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 27
- 229920001971 elastomer Polymers 0.000 claims abstract description 27
- 238000007127 saponification reaction Methods 0.000 claims abstract description 19
- 239000004744 fabric Substances 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000005303 weighing Methods 0.000 claims abstract description 6
- 239000000344 soap Substances 0.000 claims abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 239000000693 micelle Substances 0.000 claims description 6
- 235000019198 oils Nutrition 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 5
- 238000009833 condensation Methods 0.000 claims description 5
- 230000005494 condensation Effects 0.000 claims description 5
- 239000003292 glue Substances 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 7
- 238000005406 washing Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract description 2
- 229930195733 hydrocarbon Natural products 0.000 abstract description 2
- 150000002430 hydrocarbons Chemical class 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract 2
- 239000004215 Carbon black (E152) Substances 0.000 abstract 1
- 238000009835 boiling Methods 0.000 abstract 1
- 238000004898 kneading Methods 0.000 abstract 1
- 238000010992 reflux Methods 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
- 235000019483 Peanut oil Nutrition 0.000 description 2
- 239000008157 edible vegetable oil Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000312 peanut oil Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 241000221020 Hevea Species 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229920003211 cis-1,4-polyisoprene Polymers 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 150000002617 leukotrienes Chemical class 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 238000010972 statistical evaluation Methods 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- -1 this Chemical compound 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a method for determining the content of natural rubber in rubber seed oil. The determination method has the advantages of simple instrument and equipment, simple, convenient and quick operation, low detection cost and high accuracy and precision. The method for determining the content of the natural rubber in the rubber seed oil by adopting a saponification method is characterized by comprising the following steps: firstly, precisely weighing 100 to 200 grams of rubber seed oil sample and putting the rubber seed oil sample in a flask, adding potassium hydroxide into the flask to form 400 to 800 milliliters of alcohol solution, shaking up the solution and then connecting the solution to a reflux condensing tube, boiling the solution for about 1 to 2 hours in a water bath kettle, taking down the condensing tube, filtering the saponified solution to another flask while the solution is hot, crushing up rubber grains on a piece of filter cloth, washing the rubber grains till no alkali or soap is contained, finally kneading the rubber to form a slice, baking the slice with a vacuum oven (at the temperature of 80 DEG C) to constant weight and then weighing the slice, and calculating the content of the rubber. The rubber seed oil can be saponified in the alkali alcohol solution, the natural rubber belongs to a hydrocarbon substance and does not produce saponification effect, and the natural rubber is coagulated into the rubber grains to be separated out during the saponification of the rubber seed oil so as to determine the content of the natural rubber.
Description
One, technical field
The present invention relates to a kind of natural rubber Determination on content method, especially relate to natural rubber Determination on content method in a kind of rubber seed oil.
Two, background technology
Rubber seed is the oval seed of Euphorbiaceae arbor Brazil Hevea rubber capsule, plants benevolence oil-containing 40%～50%, is a kind of tropical xylophyta oil plant.Rubber seed oil belongs to semi-drying oil, and chemical composition and character and soybean oil are close, contains unsaturated fatty acid more than 80%, and wherein oleic acid 24.8%, linoleic acid 34.5%, and leukotrienes 23.3% is the higher new oil sources of a kind of nutritive value.At present domestic fundamental researchs such as rubber seed oil properties, composition are carried out.Along with the increase of rubber seed output, rubber seed oil (particularly rubber seed edible oil) exploitation causing gradually people's attention.But contain natural rubber in the rubber seed oil, human body is had certain harmfulness, seldom mention in these documents and materials in the past, the rubber content detection method does not have yet, and has influenced rubber seed oil particularly as the processing and utilization of edible oil.
Three, summary of the invention
The object of the present invention is to provide natural rubber Determination on content method in a kind of rubber seed oil, the required instrument and equipment of this method is simple, easy and simple to handle fast, detect that cost is low, accuracy and precision height, is fit to units concerned and adopts.
For achieving the above object, technical scheme provided by the invention is: adopt saponification method to carry out natural rubber assay in the rubber seed oil.Its principle is: rubber seed oil can be in alkaline ethanol solution fully saponification, natural rubber belongs to the hydrocarbons [condensate (C of isoprene
5H
8) n], saponification can not take place, in rubber seed oil saponification process, colloid is condensed into micelle and separates out, and unsaponifiables in the oil such as sterol, pigment and vitamin etc. are dissolved in alcohol, can not form solid in hot ethanol, like this, the solid micelle is separated from saponification liquor, thereby is measured its content with the method for filtering.
Said method comprises following operation steps:
(1) precision takes by weighing mixing rubber seed oil sample 100～200 and restrains in flask, adds 400～800 milliliters of potassium hydroxide system ethanolic solutions, shakes up, and connection reversed flow condensation pipe boiled on water-bath about 1～2 hour.
(2) on hot funnel, apply one 100 purpose filter cloth and make filtrator.Take off condenser pipe, the saponification liquor filtered while hot with 20～50 ml waters washing saponification flask, is poured filtrator in another flask.After having filtered, promptly get rubber on the filter cloth, part rubber is bonded on the saponification flask wall, wraps cloth with iron wire one end, and glue is all scraped off, and water washes out and is incorporated on the filter cloth.
(3) careful water washes, and micelle is crushed up, and pinches repeatedly and washes, and till alkali-free, no soap, rubber is pinched into weighed after a thin slice dries to constant weight with vacuum drying oven (80 ℃) at last, calculates rubber content.
Potassium hydroxide system ethanolic solution described in the natural rubber assay of the present invention is the ethanolic solution that contains 5%～20% potassium hydroxide.
Four, embodiment
(1) can further understand content by following specific embodiment.
Embodiment 1:
Precision takes by weighing mixing rubber seed oil sample 100 and restrains in flask, adds 400 milliliters of 5% potassium hydroxide system ethanolic solutions, shakes up, and connects the reversed flow condensation pipe, boils on water-bath 1 hour.Take off condenser pipe, saponification liquor filters in another flask with the hot funnel that applies one 100 order filter cloth in advance while hot, with 20 ml waters washing saponification flask, pours filtrator into.After having filtered, promptly get rubber on the filter cloth, part rubber is bonded on the saponification flask wall, wraps cloth with iron wire one end, and glue is all scraped off, and water washes out and is incorporated on the filter cloth.Careful water washes, and micelle is crushed up, and pinches repeatedly and washes, and till not having alkalescence, no soap, rubber is pinched into weighed after a thin slice dries to constant weight with vacuum drying oven (80 ℃) at last.
Embodiment 2:
Take by weighing mixing rubber seed oil sample 150 and restrain in flask, add 600 milliliters of 10% potassium hydroxide system ethanolic solutions, shake up, connect the reversed flow condensation pipe, on water-bath, boiled 1.5 hours.Take off condenser pipe, saponification liquor filters in another flask with the hot funnel that applies one 100 order filter cloth in advance while hot, with 30 ml waters washing saponification flask, pours filtrator into.After having filtered, promptly get rubber on the filter cloth, part rubber is bonded on the saponification flask wall, wraps cloth with iron wire one end, and glue is all scraped off, and water washes out and is incorporated on the filter cloth.Micelle is crushed up, and the water flushing does not extremely have till alkalescence, the no soap, rubber is pinched into weighed after a thin slice dries to constant weight with vacuum drying oven (80 ℃) at last.
Embodiment 3:
Basically carry out according to embodiment 1 described method, but adopt following parameter can reach reasonable measurement result:
Rubber seed oil sample 200 grams;
800 milliliters of 20% potassium hydroxide system ethanolic solutions;
On water-bath, boiled 2 hours.
(2) statistical evaluation of testing result of the present invention
1, the accuracy of assay method:
Add the natural rubber standard items with the peanut oil that does not contain natural rubber and carry out recovery test, every 100g peanut oil adds the standard rubber mass and is respectively 0.40g, 0.60g, 0.80g, and each sample is done replication, result such as table 16 times.
Table 1 is measured the data of natural rubber content accuracy
2, the precision of assay method:
Get two parts of rubber seed oils and measure the natural rubber content, 6 measured values and average measured value result such as table 2.
Table 2 precision test data table
By table 1, table 2 as can be known, this method is measured three sample additions of rubber content recovery and is all reached good effect, and RSD meets the mensuration requirement, illustrates that this method has enough accuracy and degree of accuracy.
Claims (2)
1. a method of measuring natural rubber content in the rubber seed oil is characterized in that adopting saponification method to carry out the detection of natural rubber content in the rubber seed oil, may further comprise the steps:
(1) precision takes by weighing mixing rubber seed oil sample 100～200 and restrains in first flask, adds 400～800 milliliters of potassium hydroxide system ethanolic solutions, shakes up, and connection reversed flow condensation pipe boiled on water-bath 1～2 hour;
(2) on hot funnel, apply one 100 purpose filter cloth and make filtrator, take off the reversed flow condensation pipe, the saponification liquor filtered while hot that rubber seed oil and the reaction of potassium hydroxide system ethanolic solution generate is washed first flask with 20～50 ml waters in second flask, pour filtrator into, after having filtered, promptly get rubber on the filter cloth, part rubber is bonded on first flask walls, wraps cloth with iron wire one end, glue is all scraped off, and water washes out and is incorporated on the filter cloth;
(3) careful water flushing, and micelle crushed up is pinched repeatedly and is washed, and till alkali-free, no soap, at last rubber is pinched into a thin slice, puts temperature into and is and weigh the calculating rubber content after drying to constant weight in 80 ℃ the vacuum drying oven.
2. assay method according to claim 1 is characterized in that described ethanolic solution consumption is 4～10 times of oil sample quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101310546A CN101650288B (en) | 2008-08-14 | 2008-08-14 | Method for determining content of natural rubber in rubber seed oil |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101310546A CN101650288B (en) | 2008-08-14 | 2008-08-14 | Method for determining content of natural rubber in rubber seed oil |
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| Publication Number | Publication Date |
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| CN101650288A CN101650288A (en) | 2010-02-17 |
| CN101650288B true CN101650288B (en) | 2010-12-15 |
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| CN2008101310546A Expired - Fee Related CN101650288B (en) | 2008-08-14 | 2008-08-14 | Method for determining content of natural rubber in rubber seed oil |
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| CN106092818A (en) * | 2016-08-25 | 2016-11-09 | 西双版纳华坤生物科技有限责任公司 | The assay method of natural rubber content in a kind of seeds of rubber tree oil |
| CN109793808A (en) * | 2017-11-16 | 2019-05-24 | 西双版纳华坤生物科技有限责任公司 | It is a kind of for preventing and treating the substance and purposes of parkinsonism |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1624450A (en) * | 2004-11-09 | 2005-06-08 | 中国海洋大学 | Method for Determination of Polysaccharide Content in Sea Cucumber |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1624450A (en) * | 2004-11-09 | 2005-06-08 | 中国海洋大学 | Method for Determination of Polysaccharide Content in Sea Cucumber |
Non-Patent Citations (1)
| Title |
|---|
| JP昭60-173467A 1985.09.06 |
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| CN101650288A (en) | 2010-02-17 |
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