CN1624450A - Method of determining polysaccharide content in sea cucumbus - Google Patents
Method of determining polysaccharide content in sea cucumbus Download PDFInfo
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- CN1624450A CN1624450A CN 200410036250 CN200410036250A CN1624450A CN 1624450 A CN1624450 A CN 1624450A CN 200410036250 CN200410036250 CN 200410036250 CN 200410036250 A CN200410036250 A CN 200410036250A CN 1624450 A CN1624450 A CN 1624450A
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Abstract
A method for measuring content of polysaccharide in sea cucumber, has the characteristics that the shattered sample of sea cucumber is added into water solution containing 3%-5% base which is 35-45 times of the mess of sample, then the solution is put into the super sonic wave washing instrument waiting for 1.5-2.5 hours under 40-50deg.C; use acid to adjust PH value to be neutral; the solution is centrifuged for 15-25 min by 8000-12000rpm and then get some clear liquid in which ethanol is added when being stirred to make the density of ethanol reach 55-65%; then after standing for a night; it is centrifuged for 20-30min by 4000-4500rpm and the clear liquid is abandoned and the sediment is dissolute by water in which ethanol is added into, making the density of ethanol reach 80-90%, after standing for a night; use bushing to filter and use orderly absolute ethyl alcohol and acetone to wash and anhydrate for three times; it is dried to have no change of mass and cooled to reach the room temperature, then be weighed. The invention uses secondary ethanol sediment to purify the sample and super sonic wave washing instrument as reactor, chooses bushing as mess analysis container.
Description
Technical field
The present invention relates to a kind of method of measuring polyoses content in the sea cucumber.
Background technology
Sea cucumber (Holothutia) is Echinodermata (Echinodermata) Holothuroidea (Holothurioidea).The whole world has 900 kinds approximately, and China has 140 kinds approximately; The whole world have approximately 40 kinds can be edible, about 20 kinds of China.To being regarded as helping the meals good merchantable brand, after the Ming Dynasty, sea cucumber income book on Chinese herbal medicine is classified tonic as China sea cucumber one.It is of long standing and well established that sea cucumber becomes health-preserving food, its delicious flavour, nutritious be well-known.The effect of sea cucumber polysaccharide causes domestic and international people's close attention in recent years, pharmacological research has proved that also it has multiple drug effect, as the anti-freezing reducing blood lipid, suppress platelet aggregation, prevent senile dementia, antitumor activity, the damage of control acute radiation etc., and can obviously promote the recovery and the enhancing of hematopoiesis function, be the stronger immunopotentiating agent of a kind of effect.The principal ingredient of its effect is an acid mucopolysaccharide, therefore how to judge quantitatively that the content of polysaccharide is that people pay close attention in the sea cucumber.But the method for measuring polyoses content in the sea cucumber yet there are no report.
Summary of the invention
The purpose of this invention is to provide a kind of method of measuring polyoses content in the sea cucumber, it can remedy the above-mentioned deficiency of prior art.
A kind of method of measuring polyoses content in the sea cucumber is characterized in that taking by weighing the sea cucumber sample of pulverizing, adds the aqueous solution of the 3%-5% alkali of 35-45 times of weight of sample size, reaction 1.5-2.5 hour in ultrasonic washing instrument under 40-50 ℃; Extremely neutral with sour adjust pH; With the centrifugal 15-25min of 8000-12000rpm, get supernatant; Under agitation add ethanol, make concentration of alcohol reach 55-65%, standing over night; With the centrifugal 20-30min of 4000-4500rpm, supernatant discarded night; Water dissolving gained sediment under agitation adds ethanol, makes concentration of alcohol reach 80-90%, standing over night; With the vertical crucible suction filtration that melts of constant weight, use absolute ethyl alcohol, acetone successively, each washing dehydration three times; Be dried to constant weight, cool to room temperature; Weigh.
The present invention adopts the secondary precipitation with alcohol that sample is carried out purifying; With the ultrasonic washing instrument is reactor; Select to hang down and to melt crucible and be the gravimetric analysis container, simple to operate, reliable and stable, favorable reproducibility, the accuracy height is for the mensuration of different cultivars sea cucumber polysaccharide provides reliable method.
Embodiment
Take by weighing Thelenota ananas (Jaeger) (Thelenota ananas) sample that 1 gram is pulverized, accurately to 0.0001 gram; Put into the beaker of 100ml, aqueous solution 40 grams of (concentration expressed in percentage by weight, the down with) KOH that adds 3% react 2h under 45 ℃ in ultrasonic washing instrument; With the glacial acetic acid adjust pH in the 6.8-7.2 scope; 10000rpm, 4 ℃ of centrifugal 25min get supernatant, to going in the 250ml Centrifuge Cup; Under agitation slowly add 95% ethanol, make concentration of alcohol wherein reach 60%, standing over night; With the centrifugal 30min of 4500rpm, supernatant discarded night; Sediment polysaccharide 10g dissolved in distilled water under agitation slowly adds 95% ethanol 85g, standing over night; Melt the crucible suction filtration so that constant weight G4 is vertical, use absolute ethyl alcohol, acetone successively, each washing dehydration three times; Be dried to constant weight under 50 ℃, put cool to room temperature in the exsiccator; Be weighed as 0.2031 gram.
KOH in the present embodiment can use LiOH or NaOH instead, and its effect is similar.
Method of the present invention is simple to operate, has removed remaining salt and pigment, the accuracy height.React with ultrasonic washing instrument, reduced the loss of sample.Hanging down, to melt crucible be gravimetric container, selects G4 to hang down to melt crucible to the experiment proved that the sugar in the sample is not lost, and shows that method of the present invention is simple to operate, accurately, feasible.
Claims (1)
1, a kind of method of measuring polyoses content in the sea cucumber is characterized in that taking by weighing the sea cucumber sample of pulverizing, adds the aqueous solution of the 3%-5% alkali of 35-45 times of weight of sample size, reaction 1.5-2.5 hour in ultrasonic washing instrument under 40-50 ℃; Extremely neutral with sour adjust pH; With the centrifugal 15-25min of 8000-12000rpm, get supernatant; Under agitation add ethanol, make concentration of alcohol reach 55-65%, standing over night; With the centrifugal 20-30min of 4000-4500rpm, supernatant discarded night; Water dissolving gained sediment under agitation adds ethanol, makes concentration of alcohol reach 80-90%, standing over night; With the vertical crucible suction filtration that melts of constant weight, use absolute ethyl alcohol, acetone successively, each washing dehydration three times; Be dried to constant weight, cool to room temperature; Weigh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410036250 CN1624450A (en) | 2004-11-09 | 2004-11-09 | Method of determining polysaccharide content in sea cucumbus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410036250 CN1624450A (en) | 2004-11-09 | 2004-11-09 | Method of determining polysaccharide content in sea cucumbus |
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| Publication Number | Publication Date |
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| CN1624450A true CN1624450A (en) | 2005-06-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN 200410036250 Pending CN1624450A (en) | 2004-11-09 | 2004-11-09 | Method of determining polysaccharide content in sea cucumbus |
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| CN (1) | CN1624450A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100567956C (en) * | 2005-11-30 | 2009-12-09 | 广东省农业科学院农业生物技术研究所 | A kind of lichee polysaccharide Determination on content method |
| CN101650288B (en) * | 2008-08-14 | 2010-12-15 | 海南省粮油科学研究所 | Method for determining content of natural rubber in rubber seed oil |
| CN101411523B (en) * | 2008-11-14 | 2011-04-20 | 钱武刚 | Method for extracting original liquid of trepang by ultrasonic wave |
| CN101584483B (en) * | 2008-05-19 | 2012-09-19 | 丹东阿里郎生物工程有限公司 | Method for extracting sea cucumber active liquid by ultrasonic |
| CN107536058A (en) * | 2017-07-14 | 2018-01-05 | 宁波海博士特医食品科技有限公司 | A kind of manufacture method of sea cucumber mucopolysaccharide |
-
2004
- 2004-11-09 CN CN 200410036250 patent/CN1624450A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100567956C (en) * | 2005-11-30 | 2009-12-09 | 广东省农业科学院农业生物技术研究所 | A kind of lichee polysaccharide Determination on content method |
| CN101584483B (en) * | 2008-05-19 | 2012-09-19 | 丹东阿里郎生物工程有限公司 | Method for extracting sea cucumber active liquid by ultrasonic |
| CN101650288B (en) * | 2008-08-14 | 2010-12-15 | 海南省粮油科学研究所 | Method for determining content of natural rubber in rubber seed oil |
| CN101411523B (en) * | 2008-11-14 | 2011-04-20 | 钱武刚 | Method for extracting original liquid of trepang by ultrasonic wave |
| CN107536058A (en) * | 2017-07-14 | 2018-01-05 | 宁波海博士特医食品科技有限公司 | A kind of manufacture method of sea cucumber mucopolysaccharide |
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