CN100348203C - Hypercritical soluble propolis prescription and its preparing method - Google Patents
Hypercritical soluble propolis prescription and its preparing method Download PDFInfo
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- CN100348203C CN100348203C CNB2004100148429A CN200410014842A CN100348203C CN 100348203 C CN100348203 C CN 100348203C CN B2004100148429 A CNB2004100148429 A CN B2004100148429A CN 200410014842 A CN200410014842 A CN 200410014842A CN 100348203 C CN100348203 C CN 100348203C
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- propolis
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- lecithin
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Abstract
The present invention relates to a supercritical soluble propolis liquid prescription and a process technology thereof, which belongs to the technical field of medicinal chemical industry. The prescription of the supercritical soluble propolis liquid comprises 5 to 25% of supercritical propolis liquid, 5 to 35% of perilla oil, 2 to 20% of lecithin, and 20 to 88% of honey by weight. The present invention uses a supercritical CO2 fluid extraction method for extracting pure supercritical propolis liquid from original propolis liquid. The finally manufactured propolis without dissolvent residues, heavy metal lead and other impurities is very pure and has good solubility, so the supercritical propolis liquid can be manufactured by compounding the propolis with perilla oil, honey and lecithin which have good health care effects.
Description
Technical field
The present invention relates to pharmaceutical chemistry technical field, specifically a kind of supercritical fluid extraction method of utilizing, the propolis of extraction high-purity, high concentration is as primary raw material, be equipped with other natural materials of health-care effect, that science is mixed with is high-quality, the supercritical bee-glue liquid of solubility.
Background of invention
Traditional propolis production method is an ethanol extraction method: earlier with propolis virgin rubber freezing and pulverizing powdered, the ethanol with high concentration soaks it then, uses filter screen elimination impurity behind the certain hour, the liquid that leaches, be propolis stock solution, utilize the deleading technology to remove wherein lead again, reach food sanitation standard.The problem that this class propolis liquid exists is: 1. residual solvent is many, ethanol content high this more than 60%.2. palatability is poor.3. solubility is low, and is water insoluble substantially.4. be difficult for making expensive goods etc. with other composition compatibilities.
Summary of the invention
The objective of the invention is to seek a kind of supercritical solubility propolis liquid and processing technique thereof, this propolis no solvent residue, contain heavy metal lead and other impurity hardly, very pure, have good solubility, itself and perilla oil, Mel, lecithin with health-care effect can be mixed with the favorable mouth feel supercritical bee-glue liquid.
This supercritical solubility bee-glue liquid formula is: supercritical bee-glue liquid 5～25%, and perilla oil 5～35%, lecithin 2%～20%, Mel 20%～88%, unit are percetage by weight.
Wherein supercritical bee-glue liquid is the primary raw material in the prescription, has multiple medical health care functions such as antioxidation, anti-inflammation, antiviral, blood fat reducing, vessel softening, activating cell.
Perilla oil is main compatibility raw material, contains abundant alpha-linolenic acid, and pharmacological research finds, has blood fat reducing, prevents arteriosclerosis, reduces the effects such as sickness rate, activating brain cell, prevention senile dementia of cerebral thrombosis and cardiovascular disease.
Mel also plays the mouthfeel effect of regulating in the present invention's prescription except that the function that itself has.
Lecithin is the compatibility raw material, except that function own, and the solubility effect that in the present invention's prescription, can improve prescription.
The processing technique of supercritical solubility bee-glue liquid formula is: earlier with propolis freezing and pulverizing powdered; Put it into then in the extraction kettle of supercritical extraction equipment, make CO
2Gas is in supercriticality, with CO
2The fluid form bee glue extracting, extracting pressure 16～40Mpa, extraction temperature is 35～50 ℃; Propolis after will extracting is again put into separating still, isolates the CO that is present in the supercritical propolis
2Gas gets purified supercritical bee-glue liquid, presses supercritical bee-glue liquid 5～25% again, perilla oil 5～35%, and lecithin 2%～20%, the ratio of Mel 20%～88% adds in the blender, evenly stirs into the solubility propolis liquid.Unit is a percetage by weight.
Lecithin wherein is vegetalitas or animality lecithin, and perilla oil utilizes supercritical technology extraction or traditional handicraft to extract.
Prescription of the present invention and technology are at present domesticly uniquely to take the lead in utilizing supercritical propolis to be primary raw material, the supercritical bee-glue liquid of making, advantage is that medical health care function is strong, solubility is good, mouthfeel is good, no solvent residue, and no pesticide and heavy metal are remaining, free from admixture is the propolis liquid of new generation of edible safety type.
Description of drawings
Fig. 1 is a process chart of the present invention.
The specific embodiment
Compatibility raw material perilla oil both can be selected supercritical CO for use in following each embodiment
2Extraction, perilla oil that also available traditional approach is produced, the former lecithin of compatibility can be selected vegetalitas or animality for use.Used technology is: earlier with propolis freezing and pulverizing powdered; Put it into then in the extraction kettle of supercritical extraction equipment, make CO
2Gas is in supercriticality, with CO
2The fluid form bee glue extracting, extracting pressure 16～40Mpa, extraction temperature is 35～50 ℃; Propolis after will extracting is again put into separating still, isolates the CO that is present in the supercritical propolis
2Gas gets purified supercritical bee-glue liquid, presses supercritical bee-glue liquid 5～25% again, perilla oil 5～35%, and lecithin 2%～20%, the ratio of Mel 20%～88% adds in the blender, evenly stirs into the solubility propolis liquid.
Implementation method one
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again with 5% supercritical bee-glue liquid, 5% perilla oil, 2% lecithin, 88% Mel (Unit Weight percent, as follows) put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
Implementation method two
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again 10% supercritical bee-glue liquid, 15% perilla oil, 6% lecithin, 69% Mel are put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
Implementation method three
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again 15% supercritical bee-glue liquid, 20% perilla oil, 10% lecithin, 55% Mel are put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
Implementation method four
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again 18% supercritical bee-glue liquid, 25% perilla oil, 14% lecithin, 43% Mel are put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
Implementation method five
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again 22% supercritical bee-glue liquid, 30% perilla oil, 16% lecithin, 32% Mel are put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
Implementation method six
Use supercritical CO earlier
2The fluid extraction method, from propolis stock solution, extract purified supercritical bee-glue liquid, again 25% supercritical bee-glue liquid, 35% perilla oil, 20% lecithin, 20% Mel are put into blender, utilize ultrasound wave or mechanical device to stir, up to becoming solubility propolis liquid very uniformly.
At present both at home and abroad the propolis liquid propolis liquid taste bad will, the color and luster that mainly adopt ethanol extraction technology to make is black, lead tolerance height (needing to handle through deleading), insolubility and difficult and other composition compatibilities.Be difficult for making the product of high-quality high effect.
Can overcome the above-mentioned shortcoming of existing in prior technology after utilizing the present invention: after 1. adding Mel, can improve mouthfeel, inlet has slight sweet taste.2. the color and luster yellow has the natural true qualities of bee product, compares with original black propolis, is accepted by consumer easilier.3. lead tolerance is low, and is unleaded in propolis after the employing supercritical process extracts propolis, need not increase deleading technique.4. propolis can " quantification of weighing ", makes to make degree of accuracy and be improved.5. perilla oil, the lecithin in the prescription all has effects such as blood fat reducing, enhance immunity, so propolis liquid health-care effect of the present invention is better.6. solubility is strong, and the propolis liquid that the propolis that utilizes supercritical process to extract is made has good solubility, in the beverages such as solvable entry, milk tea, coffee, bean milk, is suitable for various drunk by people; And water-soluble hardly with the propolis that is black of ethanol extraction originally, in pouring water into after, often be attached on the wall of a container, drink the comparison difficulty.
From following supercritical bee-glue liquid and ethanol propolis liquid comparison sheet, we can find out more intuitively, The relatively original ethanol propolis liquid of supercritical bee-glue liquid has obvious characteristics.
Supercritical bee-glue liquid and ethanol propolis liquid comparison sheet
| Sequence number | Project | Supercritical bee-glue liquid | The alcohol extract propolis liquid | |
| 1 | Mouthfeel | Mouthfeel is good | Mouthfeel is poor | |
| Supercritical bee-glue can attach the honey allotment, Overcome the intrinsic acid of propolis, mouthfeel Little sweet, take good to eat. | Propolis generates the polymerization of a kind of polyphenol caffeic acid after dissolving in ethanol Thing makes the propolis liquid blackout, and flavour variation is difficult to entrance, And can't merge with the additive such as honey and improve taste. | |||
| 2 | Lead content | Lead tolerance is low | The lead tolerance height | |
| The lead tolerance of state food standard code is (1mg/kg), and supercritical bee-glue liquid only is 0.08mg/g the ten minutes of not enough national regulation One of. | The general lead tolerance of ethanol propolis liquid is 8-5mg/kg, and is tight Heavily exceed standard, can not eat, thus deleading technique must be increased, Behind the deleading, though can reduce lead tolerance, can lose The propolis active ingredient increases manufacturing process and cost. | |||
| 3 | Color and luster | Yellow | Black | |
| Supercritical bee-glue adopts advanced fluid extraction Technology is carried out under low temperature, anaerobic state, Can from oxidized virgin rubber, reduce Go out pure propolis, kept the propolis true qualities. | Because oxidized dark brown virgin rubber is given birth in alcohol immersion Become a kind of polyphenol caffeic acid, the black that is similar to tar is poly-So compound is black. | |||
| 4 | Accuracy | Preparation accurately | Accuracy is low | |
| Because supercritical bee-glue can weigh in the balance Heavy, milligram calculates. | The ethanol propolis liquid is dissolved in the alcohol because of the propolis virgin rubber, and it is molten Entering amount and time is directly proportional, and is a parameter. Other one-tenth Part, DDGS cured such as the honeybee in the virgin rubber also dissolves in ethanol In the propolis liquid, so also be a parameter to concentration. | |||
| 5 | Solubility | Solubility is good | Poorly soluble | |
| Water soluble, milk tea, coffee, milk, The various beverages such as soya-bean milk are suitable for various drinking. | Substantially in the water insoluble and various liquid beverages, after the entry Namely separated out ， And invests wall of cup to propolis, can't drink. | |||
| 6 | Compatibility | Compatibility is strong | Compatibility is poor | |
| The mutual compatibility of composition of energy and various effects, Make better, the more propolis liquid of effect. | Because belonging to liquid-type (ethanol content is up to more than 60%), no Easily and other composition compatibilities, be difficult to make expensive goods. | |||
| 7 | Security | High safety | Safety | |
| Supercritical bee-glue is pure, it is residual not contain heavy metal, non agricultural chemical residuum and impurity, belongs to safety-type propolis liquid product of new generation. | The ethanol propolis liquid is essential soaked more than 1 year, former to decompose The noxious material such as bee venom in the glue, and get rid of the heavy metal that exceeds standard Lead is taken just safety. | |||
Claims (3)
1, supercritical solubility propolis liquid is characterized in that: this consists of supercritical bee-glue liquid 5～25%, perilla oil 5～35%, and lecithin 2%～20%, Mel 20%～88%, unit are percetage by weight.
2, propolis liquid according to claim 1 is characterized in that: lecithin is vegetalitas or animality lecithin, and perilla oil utilizes the supercritical technology extraction.
3, the processing technique of supercritical solubility propolis liquid is characterized in that, earlier with propolis freezing and pulverizing powdered; Put it into then in the extraction kettle of supercritical extraction equipment, make CO
2Gas is in supercriticality, with CO
2The fluid form bee glue extracting, extracting pressure 16～40Mpa, extraction temperature is 35～50 ℃; Propolis after will extracting is again put into separating still, isolates the CO that is present in the supercritical propolis
2Gas gets purified supercritical bee-glue liquid, presses supercritical bee-glue liquid 5～25% again, perilla oil 5～35%, and lecithin 2%～20%, the ratio of Mel 20%～88% adds in the blender, and unit is a percetage by weight, evenly stirs into the solubility propolis liquid.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100148429A CN100348203C (en) | 2004-04-30 | 2004-04-30 | Hypercritical soluble propolis prescription and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100148429A CN100348203C (en) | 2004-04-30 | 2004-04-30 | Hypercritical soluble propolis prescription and its preparing method |
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| CN100348203C true CN100348203C (en) | 2007-11-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101804077B (en) * | 2009-02-17 | 2012-06-27 | 中国科学院成都生物研究所 | Pretreatment method of propolis effective component raw material by supercritical extraction |
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| CN101095895B (en) * | 2007-07-06 | 2010-06-30 | 韦建学 | Externally-used medicament for the sanitation and health-care of female vagina and for treating gynecology vagina diseases and method for making the same |
| CN101731506B (en) * | 2009-12-15 | 2013-01-09 | 白万军 | Health-care food of bee glue and wood frog lecithin capsule |
| CN102188455A (en) * | 2011-05-13 | 2011-09-21 | 扬州三邦生物工程有限公司 | Method for extracting totipotent propolis |
| CN103588741A (en) * | 2013-11-15 | 2014-02-19 | 无锡尚宝生物科技有限公司 | Method for separating and purifying single high-purity flavonoid compound by using SFC (supercritical fluid chromatography) |
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| JP2001161280A (en) * | 1999-12-15 | 2001-06-19 | Fuji Oil Co Ltd | Method for making ices |
| CN1092964C (en) * | 1999-11-18 | 2002-10-23 | 钱律余 | Supercritical multiple bee glue extracting process |
| CN1389218A (en) * | 2002-07-12 | 2003-01-08 | 江苏大学 | Combined supercritical and auxiliary microwave extraction process of separating effective bee glue components |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1092964C (en) * | 1999-11-18 | 2002-10-23 | 钱律余 | Supercritical multiple bee glue extracting process |
| JP2001161280A (en) * | 1999-12-15 | 2001-06-19 | Fuji Oil Co Ltd | Method for making ices |
| CN1389218A (en) * | 2002-07-12 | 2003-01-08 | 江苏大学 | Combined supercritical and auxiliary microwave extraction process of separating effective bee glue components |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101804077B (en) * | 2009-02-17 | 2012-06-27 | 中国科学院成都生物研究所 | Pretreatment method of propolis effective component raw material by supercritical extraction |
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