CN102585999A - Method for squeezing hemp seed oil - Google Patents
Method for squeezing hemp seed oil Download PDFInfo
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- CN102585999A CN102585999A CN2012100146521A CN201210014652A CN102585999A CN 102585999 A CN102585999 A CN 102585999A CN 2012100146521 A CN2012100146521 A CN 2012100146521A CN 201210014652 A CN201210014652 A CN 201210014652A CN 102585999 A CN102585999 A CN 102585999A
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Abstract
The invention relates to a method for squeezing hemp seed oil and can effectively solve the problem of preparing the hemp seed oil from a small amount of hemp seeds to meet the requirements on medicine and research. The technical scheme adopted by the invention for solving the problem is that: the method is implemented by the following steps of: 1, processing the hemp seeds, i.e. selecting and cleaning the hemp seeds, removing impurities and foreign bodies and air-drying; 2, microwave heating, i.e. placing the processed hemp seed oil into a microwave oven to be heated; 3, squeezing and preparing the hemp seed oil, i.e. after weighing the heated the hemp seeds, repeatedly squeezing to obtain the hemp seed oil; 4, filtering, i.e. filtering the squeezed hemp seed oil and removing oil residues; and 5, disinfecting, i.e. disinfecting oil liquid obtained after the oil residues are removed and sealing and storing the oil liquid. The method is simple and scientific, is particularly suitable for preparing the hemp seed oil for the medicine and the research, which has excellent quality and high yield, is simple to operate, is suitable for the actual requirement for preparing the hemp seed oil from the hemp seeds and is an innovation on the preparation of the hemp seed oil.
Description
Technical field
The present invention relates to medicine, particularly a kind of method that is used to squeeze Hemp Seed oil.
Background technology
Hemp Seed is a conventional Chinese medicine; And be colory edible oil and healthcare products by the Hemp Seed oil that Hemp Seed obtains, also be the important source material of relevant healthcare products or medicine, the current technology that is used for the preparation of Hemp Seed oil all is industry or edible oil preparation technology; Squeeze after employing cold-press, the frying etc.; Tens kilograms of general requirement inlet amounies even more, even particular requirement is arranged, charging also needs more than 2 kilograms.The technology of especially existing heating adopts naked light, cauldron frying, causes the sample heating inhomogeneous easily, and raw material excessively is heated the oil quality that squeezes is descended, and is not easy to prepare best in quality, the oil plant that yield is high used as medical research.In addition, also require needs simple to operate, as to be fit to the minute quantity sample.
Be focus to researchs such as the oily composition analysis of Hemp Seed, pharmacological actions at present, therefore, preparing Hemp Seed oil best in quality, that yield is high is to carry out the prerequisite of later stage research with crucial.This technology should adapt to the current application requirement; Except excellent product quality, yield height, also require preparation simple to operate, as to be fit to the minute quantity sample.Yet, be not specifically designed to the preparation method of experimental study with Hemp Seed oil, especially do not find to adopt microwave heating method to prepare the method for Hemp Seed oil.
Summary of the invention
To above-mentioned situation, for overcoming the defective of prior art, the present invention's purpose just provides a kind of method that is used to squeeze Hemp Seed oil, can effectively solve a small amount of Hemp Seed and prepare Hemp Seed oil, to satisfy the problem of medicine and research needs.
The technical scheme that the present invention solves is that this method is realized by following steps:
1, Hemp Seed is handled: choose Hemp Seed and clean, remove impurity and foreign matter, dry;
2, microwave heating: the Hemp Seed after handling inserted in the microwave oven heat;
3, squeezing, preparation Hemp Seed oil: after the Hemp Seed after the heating was weighed, squeezing squeezed out Hemp Seed oil repeatedly;
4, filter: the Hemp Seed oil that squeezes out is filtered, remove the dregs of fat;
5, sterilization: after removing fluid behind the dregs of fat and carrying out disinfection, airtight preservation.
Simple, the science of the inventive method, be particularly useful for making medicine and research usefulness best in quality, yield is high, and simple to operate, be fit to the actual needs that a small amount of Hemp Seed prepares Hemp Seed oil, be the innovation on the preparation Hemp Seed oil.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
The screw press sectional front view of Fig. 2 for using among the present invention.
Embodiment
Elaborate below in conjunction with the particular case specific embodiments of the invention.
Provided by Fig. 2, the present invention is to be realized by following steps in practical implementation:
1, Hemp Seed is handled: choose Hemp Seed and use the water purification wash clean, remove impurity and foreign matter, dry;
2, microwave heating: the Hemp Seed after processing dried is inserted in the microwave oven and is heated microwave oven oscillation frequency 2450MHz, microwave output power 500W (producing 80～100 ℃), 20 minutes heat-up times;
3, squeezing, preparation Hemp Seed oil: the 20g that weighs of the Hemp Seed after the heating, insert in the screw press; Under the pressure of pressure 500-1000Kg, squeeze 5-8 time repeatedly, the pressure of squeezing is along with the squeezing number of times progressively increases; Squeeze fuel-displaced, with guarantee oil yield >=21% (it is to be noted, big like needs Hemp Seed oil mass; Can be divided into repeatedly squeezing, each 20g Hemp Seed);
4, filter: the Hemp Seed oil that squeezes out is placed in the clean Glass Containers, pour the funnel inner filtration that aseptic absorbent cotton is arranged at the bottom into, remove the dregs of fat;
5, sterilization: the Hemp Seed fluid after the filtration is loaded in the clean brown vial, behind the routine disinfection, and airtight preservation under 1-4 ℃ of low temperature (described routine disinfection can adopt conventional disinfection by ultraviolet light or adopt 80-120 ℃ of high-temperature sterilization).
Described screw press includes cylindric cylinder body, piston, screw rod and pressure arm, and piston 6 is contained in the cylinder body 5, and cylinder body is contained on the fixed link 7 of base 8; The bearing bottom plate 9 of taking oilhole out of is arranged in the cylinder base, be contained in end socket 3 through screw 4 in the upper oral part of cylinder body, there is threaded hole at the end socket center; Be installed with screw rod 2 in the threaded hole; Screw rod 2 bottoms and piston are fixed together, and screw rod stretches out end socket top arm 1 is housed, described diameter of cylinder 60mm, wall thickness 3mm, high 100mm.
Technology of the present invention and equipment are simple, use easy to operate, and quality product is high; Yield is high, is particularly suitable for the preparation of the Hemp Seed oil samples that a small amount of medical and scientific research use, and the present invention tests through repeated multiple times (12 times); All obtain identical or akin result, shown that method is reliable and stable, had practicality; And the warp test has obtained fully proving that relevant testing data is following:
1. microwave heating method is squeezed Hemp Seed oil
1.1 test materials
Hemp Seed is purchased the medicinal material company in the Bozhou, Anhui, meets " Chinese pharmacopoeia standard through identifying.
1.2. experimental technique
1.2.1 take by weighing Hemp Seed medicinal material (shelling) 20g, be tiled on the suitable vessel of microwave oven, the duration and degree of heating and time are set to desired location (reference table 1), begin heating then.After heat-processed finishes, take out Hemp Seed, weighing and recording is so that calculate the moisture that loses in the heat-processed.(concrete data see Table 2)
1.2.2 fixedly squeezing condition (with Hemp Seed, be tiled in the cylinder base that diameter is 6cm, adopt the pressure of top pressurization 500Kg to continue 2min, 3 times repeatedly) is investigated the influence to milling process of application of sample amount, microwave output power, heat-up time.With the oil yield is to investigate index, adopts L
9(3
4) orthogonal table, carried out 9 experiments altogether.Level of factor is seen table 1.
Table 1 level of factor
Explain: in application, the laboratory sample amount is generally 10～20g, is 10g, 15g, three investigation levels of 20g so establish sample size; Conventional microwave oven microwave output power is 200～1000 watts, and the gear that is lower than 500W in the preliminary experiment just can make oil yield increase heat-up time more than one hour, and efficient is obviously low, so set 500W, 650W, three investigation levels of 800W; Surpass the situation that the sample over-heating possibly appear in 30min heat-up time on selected low-grade location, take into account efficiency simultaneously and consider, sets 10min, 15min, three levels of 20min.
1.2.3 Orthogonal experiment results is seen table 2.
Table 2 Hemp Seed microwave method is handled the L of Hemp Seed
9(3
4) Orthogonal experiment results
1.2.4 table 3 is seen in the Orthogonal experiment results variance analysis
The variance analysis of table 3 Orthogonal experiment results
* showing has significance
Can know that by The results of analysis of variance the influence to milling process of sample size and time has significance, can know that by the result of intuitive analysis optimum process condition is A
3B
1C
3Oil yield is 21%.Be sample size 20g, microwave output power is 500W (2450MHz), and the time is 20min.
2. measure Hemp Seed oil iodine number, estimate the quality (content of unsaturated fatty acids) of oil
The content of unsaturated fatty acids degree of unsaturation commonly used is weighed in the grease, and greasy degree of unsaturation is represented with oil iv.So-called oil iv is meant under prescribed condition, the gram number of required iodine in the addition reaction of every 100g grease, and iodine number is high more, explains that greasy degree of unsaturation is big more, and the content of unsaturated fatty acids is high more.New expressed Hemp Seed oil is green-yellow, put for a long time then become isabelline.
The oil iv method for measuring has multiple, and wherein the iodine chloride additive process is called the Webster additive process again, and that this method has is easy, quick, accurately reach condition is easy to advantages such as control, is the most frequently used method of vegetable and animals oils determination of iodine value.
2.1. experimental principle: make the unsaturated double-bond in excessive iodine chloride (Webster liquid) and the grease carry out quantitative addition reaction:
After reacting completely, add liquor kalii iodide and remaining iodine chloride effect and separate out iodine, make indicator with the shallow lake, fixed with sodium thiosulfate standard solution, do blank test simultaneously.
Can try to achieve greasy iodine number calculation formula according to the amount of the used sodium thiosulfate standard solution of titration:
Oil iv=(V
0-V) * 1.269/m
In the formula: V
0Blank test consumes the volume of sodium thiosulfate standard solution, ml
V grease sample consumes the volume of sodium thiosulfate standard solution, ml
The gram number of the iodine that 1ml 0.1mol/L sodium thiosulfate standard solution is suitable equals 1.269
The quality of m grease sample, g
2.2. experimental section
2.2.1 laboratory apparatus and reagent:
Experiment material: iodine flask (500ml); Transfer pipet (25ml); Base buret (50ml); Drop bottle (60ml); Brown reagent bottle (1000ml); Table scale; Analytical balance;
Reagent: trichloromethane (analytical pure); Potassiumiodide (analytical pure); Sulfothiorine (Na
2S
2O
35H
2O, analytical pure); SRM 935a (analytical pure); Glacial acetic acid (analytical pure); Iodine (analytical pure); Iodine chloride (analytical pure); Potassiumiodide (10%): platform balance takes by weighing 10 gram potassiumiodide solids and is dissolved in 100ml water; Starch (1%): after platform balance takes by weighing 1 gram starch is dissolved in 100ml boiling water, cooling; Sodium thiosulfate standard solution (0.1molL
-1): platform balance takes by weighing 25 gram Sulfothiorine (Na
2S
2O
35H
2O, analytical pure) being dissolved in 300ml newly boils refrigerative zero(ppm) water, adds 0.2 gram yellow soda ash (Na
2CO
3) after the dissolving, with newly boiling the refrigerative distilled water diluting to 1L, store in the brown bottle, place and demarcate with SRM 935a after 7 days; Webster liquid (iodine chloride glacial acetic acid solution): take by weighing 16.5 gram iodine monochlorides and be dissolved in the 1000ml glacial acetic acid, change in the brown reagent bottle and keep in Dark Place.
Sample: Hemp Seed oil
2.2.2 experimental procedure:
2.2.2.1 sample determination takes by weighing 0.1g Hemp Seed oil samples in the 500ml iodine flask with the decrement method, adds the 10ml trichloromethane, shakes gently to make the oil sample dissolving; Accurately add 25.00ml Webster liquid, the jam-pack bottle stopper, and with a small amount of potassiumiodide fluid-tight mouth; Shake up back (20 ℃ of room temperatures) reaction 60 minutes, take out along bottleneck and add 10ml 10% liquor kalii iodide, shake a little in the dark place; Behind 100ml water flushing bottle stopper and bottleneck, use 0.1molL
-1The sodium thiosulfate standard solution titration is to faint yellow, and adding 1ml 1% a starch solution continuation extremely blue disappearance just is terminal point, writes down the volume of used sodium thiosulfate standard solution.
2.2.2.2 the sample determination similarity condition is pressed in blank determination, non-refuelling appearance is operated, and writes down the volume of used sodium thiosulfate standard solution.
2.3 experiment condition and control
The amount of taking of grease sample should be confirmed based on estimating with reference to iodine number, like table 4
Table 4 Webster method is measured iodine number sample reference mass table
Annotate: the quality of sample must can guarantee that the Webster liquid of adding is excessive 50%～60%, promptly 100%～150% of absorbed dose.According to reference, this experiment Hemp Seed oil 0.1g that materialses.
2.4. experimental data and result calculate
Hypo solution calibration result: c=0.09913molL
-1(seeing table 5)
Table 5 hypo solution nominal data
Table 6 microwave method squeezing Hemp Seed oil determination data and result
Can find out intuitively that through table 6 each batch Hemp Seed oil iodine number difference is little, through statistical procedures, each batch Hemp Seed oil iodine number result's variation very little (RSD=1.6%), showing does not have difference between the numerical value.Method of proof is reliable and stable, and good suitability is arranged, and has practical value.
2.5 conclusion
Through the analysis to experimental result, definite optimised process is: raw material (Hemp Seed shells) sample size is 20g, microwave method: microwave output power is 500W (2450MHz), and be 20min heat-up time.Confirmatory experiment has shown this process stabilizing, and craft science is feasible, can satisfy the requirement that the laboratory is few with sample size, precision is high and oil yield is high, quality is good.Be that the innovation on the Hemp Seed oil is squeezed in the Hemp Seed heating.
Claims (1)
1. a method that is used to squeeze Hemp Seed oil is characterized in that, is realized by following steps:
(1) Hemp Seed is handled: choose Hemp Seed and use the water purification wash clean, remove impurity and foreign matter, dry;
(2) microwave heating: the Hemp Seed after processing dried is inserted in the microwave oven and is heated microwave oven oscillation frequency 2450MHz, microwave output power 500W, 20 minutes heat-up times;
(3) squeezing, preparation Hemp Seed oil: the 20g that weighs of the Hemp Seed after the heating, insert in the screw press; Under the pressure of pressure 500-1000kg, squeeze 5-8 time repeatedly, the pressure of squeezing is along with the squeezing number of times progressively increases; Squeeze fuel-displaced, to guarantee oil yield >=21%;
(4) filter: the Hemp Seed oil that squeezes out is placed in the clean Glass Containers, pour the funnel inner filtration that aseptic absorbent cotton is arranged at the bottom into, remove the dregs of fat;
(5) sterilization: the Hemp Seed fluid after the filtration is loaded in the clean brown vial, behind the routine disinfection, and airtight preservation under 1-4 ℃ of low temperature.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026260A (en) * | 2013-04-01 | 2014-09-10 | 洛阳春魁农业开发有限公司 | Peony seed blend oil containing hemp seed oil and preparation method thereof |
| CN105001979A (en) * | 2015-07-24 | 2015-10-28 | 黑龙江惠民兴生物科技有限公司 | Method for preparing hempseed oil through physical squeezing |
| CN105505563A (en) * | 2015-12-15 | 2016-04-20 | 湖南林邑新能源有限公司 | Acidic tung oil production technology |
| US10143706B2 (en) * | 2016-06-29 | 2018-12-04 | Cannscience Innovations, Inc. | Decarboxylated cannabis resins, uses thereof and methods of making same |
| CN110680024A (en) * | 2019-10-10 | 2020-01-14 | 黑龙江黑科科技有限责任公司 | Preparation method of cannabis electronic tobacco shreds and cannabis electronic cigarette cartridge |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1714661A (en) * | 2004-07-01 | 2006-01-04 | 卜云萍 | High gamma-linoleic acid edible oil and its preparing method and use |
| CN101177647A (en) * | 2007-04-18 | 2008-05-14 | 广西巴马常春藤生命科技发展有限公司 | Laportea urentissima grease processing technology |
| CN101597542A (en) * | 2008-06-03 | 2009-12-09 | 周晓南 | Microwave treatment and extruding puffing synthesis method prepare the processing method of rubber seed oil |
| CN201722357U (en) * | 2009-09-11 | 2011-01-26 | 许龙 | Integrated microwave oil expeller |
-
2012
- 2012-01-16 CN CN2012100146521A patent/CN102585999A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1714661A (en) * | 2004-07-01 | 2006-01-04 | 卜云萍 | High gamma-linoleic acid edible oil and its preparing method and use |
| CN101177647A (en) * | 2007-04-18 | 2008-05-14 | 广西巴马常春藤生命科技发展有限公司 | Laportea urentissima grease processing technology |
| CN101597542A (en) * | 2008-06-03 | 2009-12-09 | 周晓南 | Microwave treatment and extruding puffing synthesis method prepare the processing method of rubber seed oil |
| CN201722357U (en) * | 2009-09-11 | 2011-01-26 | 许龙 | Integrated microwave oil expeller |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026260A (en) * | 2013-04-01 | 2014-09-10 | 洛阳春魁农业开发有限公司 | Peony seed blend oil containing hemp seed oil and preparation method thereof |
| CN104026260B (en) * | 2013-04-01 | 2016-03-02 | 洛阳春魁农业开发有限公司 | A kind of peony seeds ready-mixed oil containing fructus cannabis oil and preparation method thereof |
| CN105001979A (en) * | 2015-07-24 | 2015-10-28 | 黑龙江惠民兴生物科技有限公司 | Method for preparing hempseed oil through physical squeezing |
| CN105001979B (en) * | 2015-07-24 | 2019-01-22 | 黑龙江惠民兴生物科技有限公司 | A kind of method that physical squeezing prepares hemp-seed oil |
| CN105505563A (en) * | 2015-12-15 | 2016-04-20 | 湖南林邑新能源有限公司 | Acidic tung oil production technology |
| US10143706B2 (en) * | 2016-06-29 | 2018-12-04 | Cannscience Innovations, Inc. | Decarboxylated cannabis resins, uses thereof and methods of making same |
| US10383892B2 (en) | 2016-06-29 | 2019-08-20 | CannScience Innovations Inc. | Decarboxylated cannabis resins, uses thereof and methods of making same |
| US10537592B2 (en) | 2016-06-29 | 2020-01-21 | CannScience Innovations Inc. | Decarboxylated cannabis resins, uses thereof and methods of making same |
| CN110680024A (en) * | 2019-10-10 | 2020-01-14 | 黑龙江黑科科技有限责任公司 | Preparation method of cannabis electronic tobacco shreds and cannabis electronic cigarette cartridge |
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Application publication date: 20120718 |